Editing BioMicroCenter:PricingFY2013 (section)

== [[BioMicroCenter:Sequencing|ILLUMINA SEQUENCING]] ==

=== SEQUENCING ===
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on the Genome Analyzer.

{| border=1 align="left"
 ! ILLUMINA SEQUENCING:HiSeq
 !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]/MIT
 !width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 !width=100| unit
 ! Notes
 |-
 | colspan="5" align=center |<BR>'''HiSEQ 2000''' sequencing (minimum 100m reads/lane. Average ~160-240m reads/lane)
 |-
 | Single End (40nt)
 |align="right"| $1,170
 |align="right"| $1,755
 |align="center"| per lane
 | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of fastq files for 2 yrs.
 |-
 | Paired End (40+40nt)
 |align="right"| $1,850
 |align="right"| $2,775
 |align="center"| per lane
 | 
 |-
 | Add'l 40nt
 |align="right"| $370
 |align="right"| $555
 |align="center"| per lane
 |  
 |-
 | colspan="5" align=center |<BR>'''MiSeq''' sequencing (minimum 2m reads/lane. Average ~5m reads/lane)
 |-
 | 50nt Run
 |align="right"| $1,000
 |align="right"| $1,500
 |align="center"| per lane
 | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of fastq files for 2 years
 |-
 | 300nt Run (PE or SE)
 |align="right"| $1,300
 |align="right"| $1,950
 |align="center"| per lane
 | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of fastq files for 2 years
 |-
 | 500nt Run (PE or SE)
 |align="right"| $1,450
 |align="right"| $2,175
 |align="center"| per lane
 | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of fastq files for 2 years
 |- 
 | colspan="5" align=center |<BR>'''GAII / GAIIx''' sequencing (Average ~25-40m reads/lane)
 |-
 | colspan="5" align=center |''GAII Services are now completely a la carte to allow us to run partial lanes. No guarantees are made on partial flowcell runs. Price includes data storage for two years but does not include quality control. PhiX lanes must be included in your purchased lanes if you want to use control normalization.''
 |-
 | 8 Lanes (SE/PE)
 |align="right"| $4,835 / $7,135
 |align="right"| $7,253 / $10,702
 |align="center"| 1 day
 | 24nt per day. 42nt per seq kit.
 |-
 | 4 Lanes (SE/PE)
 |align="right"| $3,465 / $4,700
 |align="right"| $5,198 / $7,050
 |align="center"| 1 day
 | 36nt per day. 66/33* nt per seq kit.
 |-
 | 2 Lanes (SE/PE)
 |align="right"| $2,780 / $4,015
 |align="right"| $4,170 / $6,023
 |align="center"| 1 day
 | 48nt per day. 106/54* nt per seq kit.
 |-
 | 1 Lane (SE/PE)
 |align="right"| $2,440 / $3,675
 |align="right"| $3,660 / $5,513
 |align="center"| 1 day
 | 72nt per day. 140/81* nt per seq kit.
|-
| colspan="5" align=center |&#42; ''Second number pertains to reads greater than 40 nt.''
 |-
 | Add'l Day
 |align="right"| $325
 |align="right"| $488
 |align="center"| 1 day
 | covers service contract and data storage requirements.
 |-
 | Add'l Sequencing Kit
 |align="right"| $1,400
 |align="right"| $2,100
 |align="center"| 1 Kit
 | 
 |-
 | colspan="5" align=center |<BR>'''Additional Services''' 
 |- 
 | colspan="5" align=center |''These services apply to samples submitted for all platforms.''
 |- 
 | Sample Multiplexing
 |align="right"| $50
 |align="right"| $75
 |align="center"| per 10 samples
 | Combined over the whole order. Only applies to samples combined by BMC technicians.
 |-
 | Add'l Repriming
 |align="right"| $200
 |align="right"| $300
 |align="center"| per flow cell
 | Uses 6-8nt. Cost is divided by number of lanes requiring repriming. Repriming is required for standard Illumina multiplexing.
 |-
 | Quality Control Only
 |align="right"| $30
 |align="right"| $45
 |align="center"| per sample
 | Includes RT-PCR and BioAnalyzer
 |}
<BR>

<BR>
Prices effective 7.1.2012 <BR><BR>
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on the Genome Analyzer. The BioMicro Center reserves the right to reject samples for sample preparations.

=== SAMPLE PREPARATION ===

{| border=1 align="left"
 ! ILLUMINA Sample Preparation
 !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]/MIT
 !width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 !width=100| unit
 ! Notes
 |-
 | colspan="5" | '''STARTING WITH DNA''' 
 |-
 | Full Service Sheared DNA (SPRIWORKS)
 |align="right"| $150
 |align="right"| $225
 |align="center"| per sample
 | Includes adapter ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
 |-
 | ''SPRI-TE use only''
 |align="right"| $100
 |align="right"| N/A
 |align="center"| per sample
 | User must provide all primers. We do not QC the samples. Samples will be returned for amplification.
 |-
 | Full Service Intact Genomic DNA (NEXTERA)
 |align="right"| $150
 |align="right"| $225
 |align="center"| per sample
 | Includes adapter ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
 |-
 | colspan="5" | '''STARTING WITH RNA''' 
 |-
 | Illumina TruSeq 
 |align="right"| $300
 |align="right"| $450
 |align="center"| per sample
 | Preformed using TruSeq AND SPRIworks system. <br> Includes purifying mRNA, generation of fragmented dsDNA, adaptor ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
 |-
 |''+strand specific''
 |align="right"| $30
 |align="right"| $45
 |align="center"| per sample
 | Requires 1ug of RNA. Uses the dUTP method.
 |-
 | Epicenter Ribozero<br>''no polyA pulldown''
 |align="right"| $350
 |align="right"| $525
 |align="center"| per sample
 | Requires 1ug of RNA. Based on Illumina Truseq chemistry
 |-
 | Low Input (Clontech)
 |align="right"| $375
 |align="right"| $575
 |align="center"| per sample
 | Can work on single cells
 |-
 | Low Input (NuGen)
 |align="right"| $400
 |align="right"| $600
 |align="center"| per sample
 | Can work on degraded RNA.
 |-
 | Tight Size Cut
 |align="right"| $75
 |align="right"| $125
 |align="center"| per pool
 | +/- 40bp using PippinPrep
 |-
 | colspan="5" | '''STARTING WITH CHROMATIN''' Note: User must supply antibodies.
 |-
 | Full Service ChIP-seq
 |align="right"| $210
 |align="right"| $315
 |align="center"| per sample
 | Preformed using IP-Star AND SPRIworks system. <br> Includes ChIP, library contruction and barcoding.
 |-
 | ''IP-Star Only''
 |align="right"| $70
 |align="right"| $105
 |align="center"| per sample
 | Preformed using IP-Star
 |-
 | ''qPCR of ChIP''<br> ''in development''
 |align="right"| TBA
 |align="right"| TBA
 |align="center"| per sample
 |Tests 8 IPs against 5 genomic loci in duplicate. User provides oligos.
 |-
|}
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