Editing BioMicroCenter:MicroArrayTemp (section)

== BioMicro Center Services ==
We are currently set up to primarily handle expression analysis. Our service include:

* Evaluation of sample quality using the [[BioMicroCenter:2100BioAnalyzer|Agilent BioAnalyzer 2100]]
* Sample labeling and clean up.
* Array Hybridization.
* Data extraction and normalization.

We also have discounts with different vendors and can help you purchase your arrays.
<br>
=== SAMPLE TYPES ===
The BioMicro Center has extensive experience handling samples for expression analysis.

{|border=1
 |-
 ! Eukaryote
 ! Min.RNA
 ! Min.Vol.
 ! Notes
 |-
 | Prokaryote
 | 1 μg
 | 6 μL
 | labeling using [http://www.ambion.com/catalog/ProdGrp.html?fkProdGrp=339 Ambion] for Affy arrays
 |-
 | 3' Array (std)
 | 100 ng 
 | 6 μL
 | labeling using NuGEN for Affy arrays. 
 |- 
 | Exon Array
 | 100 ng
 | 6 μL
 | labeling using NuGEN for Affy arrays
 |- 
 | small RNA
 | N/A
 | N/A
 |BMC recommends the use of multiplexed [[BioMicroCenter:Sequencing|Illumina sequencing]] for small RNA samples
|}
<br>
=== SAMPLE SUBMISSION ===

We encourage researchers to contact us several days in advance of sample submission to allow us lead time to purchase any specialized Affymetrix or Agilent arrays. While we keep a few arrays in stock, most arrays are ordered on an as needed basis. A list of some of the available arrays and their prices can be found [[Media:Affy_Arrays_081218.pdf| in this file]]. Information about Affymetrix arrays is available at http://www.affymetrix.com/products_services/index.affx

All submissions must be accompanied by a [[BioMicroCenter:Forms#Downloads | Affymetrix Submission Form]]. 
<br>
=== TURN AROUND ===
The BioMicro Center will turn around your samples as quickly as possible. We are currently exploring methods of increasing our sample throughput to reduce turn around time. Average turn around time is currently 2-3 weeks.
<br>
=== NON-MIT USERS ===
Please contact [[BioMicroCenter:People|Manlin Luo]] to arrange a drop off date and time. Samples on dry ice and chips at room temperature with a completed order form are shipped by overnight delivery to:
		
   MIT BioMicro Center
   Attention: Manlin Luo
   31 Ames Street, Building 68-316
   Cambridge, MA 02139
<br>
=== [[BioMicroCenter:Prices|PRICES]] ===
Please note that processing prices '''do not''' include the cost of the array.  In order to be sure we have arrays on hand, we encourage users to contact us several days in advance of submitting samples.

{| border=1  style="text-align:center" 
 ! ITEM 
 ! style="width:100px"|[[CoreDeps|CORE LAB]] 
 ! style="width:100px"| MIT 
 ! style="width:100px"| NON-MIT 
 ! style="width:100px"| unit
 |-
 | Affy 3' Processing || $260 || $430 || $645 || per array 
 |-   
 | Affy Exon Processing || $300 || $535 || $800 || per array 
 |-
 | Affy Prokaryote || $260 || $430 || $645 || per array
 |-
 | Agilent Arrays || (inquire) || || ||
|}

Common Affymetrix Arrays (some in stock):
* Human U133 Plus 2.0 / Mouse 430 2.0 - '''$400'''
* Human U133A 2.0 / Mouse 430A 2.0 - '''$250''' 
* Human / Mouse Exon 1.0 ST - '''$400'''
* Yeast 2.0 - '''$175'''
Full Price List: [[Media:Affy_Arrays_081218.pdf| Affymetrix Prices]]

 ** Please note: Processing prices will be changing on July 1, 2009 **