Editing BioMicroCenter:FemtoPulse (section)

== Ultra High Sensitivity/Genomic DNA ==

The FP analyzes DNA using pulse-field capillary electrophoresis. A tiny amount of sample is injected through a capillary with gel, and across a fluorescence detection window using a voltage gradient. DNA intercalating dye within the gel makes the DNA detectable by a camera lens, which is able to read the fluorescence levels of the fragments through the window, and accurately give the size and concentrations of the sample.  Unlike the Fragment Analyzer, the pulse-field generator of the FP enables sizing of much longer DNA fragments, up to 200kbp in length.


The FA processes DNA fragments of 50bp-200kbp at a wide range of concentrations:

0.1pg/μL-100pg/μL (Fragments)

2.5pg/μL-500pg/μL (Smear)

0.3pg/μL-30pg/μL (gDNA Fragment)

5pg/μL-500pg/μL (gDNA Smear)

Higher inputs can be handled using the [[BioMicroCenter:Advanced_analytical_Fragment_analyzer|Fragment Analyzer]].