Editing BioMicroCenter:DNA LIB (section)

== NEB Ultra II ==
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  !Parameter 
  !Requirements
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  |INPUT || Clean fragmented DNA <BR> 100pg-100ng <BR> >10uL
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  |INCLUDED || Initial QC by Fragment Analyzer <BR> Library preparation <BR> Illumina QC
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  |SEQUENCING RECOMMENDATIONS || All platforms
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  |SUBMISSION || MIT - [https://mit.ilabsolutions.com/service_item/new/3381?spt_id=3863 ilabs] <BR> External - [[BioMicroCenter:Forms|form]]
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  |UNIT || Per sample
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  |PRICING ||[[BioMicroCenter:Pricing#ILLUMINA_LIBRARY_-_DNA|LINK]]
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The BioMicro Center utilizes the [https://www.neb.com/products/e7645-nebnext-ultra-ii-dna-library-prep-kit-for-illumina#Product%20Information NEB UltraII] kit for standard library preparation. This kit is extremely robust and can make libraries from <1ng of input material. It is also amenable to miniaturization and is available as a high-throughput prep as well. NEB Ultra II is based on standard LM-PCR based chemistry. DNA fragments are repaired, A-tailed and ligated to adapters. The ligated products are then amplified by PCR to add indexes and NGS short read anchors. Completed libraries can be size selected using SPRI beads or BluePippin.
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[[image:NEB_Data.jpg|thumb|right|NEBNext Ultra II produces the highest rates of conversion to adaptor-ligated molecules from a broad range of input amounts.]]

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