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BioMicroCenter:BioAnalyzer INFO
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==DNA== The BioAnalyzer analyzes DNA using the same principles as slab agarose gels. A very small tube of gel is created and a fraction of the DNA loaded, along with a low and high size marker, and are placed at one end of the chip. Then, using a voltage gradient, the sample is run down the length of the chip. As the DNA passes a laser, it is detected. Subsequent samples are run down the same lane of the chip. Quantification is performed relative to the sizing standards. Only 1 uL of sample is required. The high sensitivity assay is preferred, and samples are diluted to within range unless otherwise requested. [http://www.chem.agilent.com/en-us/products/instruments/lab-on-a-chip/2100bioanalyzer/dnasolutions/pages/default.aspx DNA] - [http://www.chem.agilent.com/en-us/products/instruments/lab-on-a-chip/pages/gp1565.aspx How it works] ''(Agilent)'' There are four different options for DNA arrays: * DNA 1000 Kit: 25bp - 1,000bp, 0.1-50ng/uL * DNA 7500 Kit: 100bp - 7,500bp, 0.1-50ng/uL * DNA 12000 Kit: 100bp - 12,000bp, 0.1-50ng/ul * High Sensitivity DNA kit: 50bp - 7,000bp, 5-500pg/ul The BioMicro Center keeps only the High Sensitivity DNA kit in stock.
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