BioMicro Center - User contributions [en]

MIT:Pricing

2026-04-27T17:27:20Z

Hilo1: /* OTHER LAB SERVICES */

'''PRICING UPDATE 7/1/2025'''

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850 || $2,000 || rowspan="2" | Per Lane || rowspan="5" | Through collaboration with nearby academic shared resources
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600 || $2,860
|-
| NovaSeqX 10B 300nt^^ || $12,160 || $13,376 || rowspan="3" | Per Flowcell
|-
| NovaSeqX 25B 300nt^^ || $16,200 || $17,820
|-
| NovaSeqX 25B 100nt^^ || $5,750 || $6,325
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| rowspan="2" | 75 PE || Full flowcell (2 lane, 800m read) || $1,900 || $2,090 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| One lane (~400m read) || $1,000 || $1,100 || per lane
|-
| rowspan="2" | 150 PE || Full flowcell (2 lane, ~800m read) || $2,650 || $2,915 || per flowcell
|-
| One lane (~400m read) || $1,375 || $1,513 || per lane
|-
| 300 PE || Full flowcell (~200m reads) || $3,500 || $3,850 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100m reads) || $300 || $330 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell (~400m reads)* || $1,800 || $1,980 || per flowcell
|-
| 150 PE: F3 Lane (~100m reads) || $450 || $495 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

{| class="wikitable"
|-
! Length !! Reads !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 300nt || rowspan="2" | 5M || $715 || $786.50 || rowspan="9" | per kit || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || $1,040 || $1,144
|-
| 100nt || rowspan="4" | 25M || $925 || $1,017.50
|-
| 300nt || $1,334 || $1,467.40
|-
| 600nt || $1,570 || $1,727
|-
| '''1000nt''' || $2,590 || $2,849
|-
| 100nt || rowspan="2" | 100M || $1,540 || $1,694
|-
| 300nt || $2,125 || $2,337.50
|-
| 600nt || 50M || $2,125 || $2,337.50
|}

==== MiSeq i100 Walkup ====
Training and lab storage on BMC servers required. Run must be scheduled in the [https://mit.ilabsolutions.com/schedules/555535#/schedule/ iLabs calendar.]

{| class="wikitable"
|-
! Length !! Reads !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! RunTime !! Notes
|-
| Machine usage || — || $120 || $132 || per run || — || training and lab storage on BMC servers required.
|-
| 300nt || rowspan="2" | 5M || $381 || $419.10 || rowspan="9" | per kit || 8h || rowspan="9" |
|-
| 600nt || $665 || $731.50 || 16h
|-
| 100nt || rowspan="4" | 25M || $565 || $621.50 || 5h
|-
| 300nt || $920 || $1,012 || 8h
|-
| 600nt || $1,125 || $1,238 || 16h
|-
| 1000nt || $1,921 || $2,113.10 || 24h
|-
| 100nt || rowspan="2" | 100M || $1,130 || $1,243 || 5h
|-
| 300nt || $1,638.50 || $1,802.35 || 8h
|-
| 600nt || 50M || $1,638.50 || $1,802.35 || 16h
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! Length !! Reads !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 70nt || 15M || $1,320 || $1,452 || rowspan="6" | per kit || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt || 25M || $1,430 || $1,573
|-
| 300nt || 15M || $1,595 || $1,754.50
|-
| 500nt || 15M || $1,760 || $1,936
|-
| 600nt || 25M || $2,200 || $2,420
|-
| 500nt || 1M  '''NANO''' || $770 || $847
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only -only one QC is included per sequencing lane. || $35 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate(short read) || $400 || $440 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200 || $1,320 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| SMRT Cell || $2,900 || $3,190 || per SMRT Cell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! colspan="3" | ILLUMINA Sample Preparation !! colspan="3" style="color:blue;" |  
|-
| colspan="3" style="font-weight:bold; text-align:center;" | '''STANDARD THROUGHPUT DNA'''   Prices for individual samples. Repreps will typically be attempted on failed samples without charge. || colspan="3" style="font-weight:bold; text-align:center; color:blue;" | '''HIGH THROUGHPUT DNA'''   Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| rowspan="3" | LIGATION BASED NEB UltraII || STD || per sample || $105 || $115 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| style="color:blue;" | HTL || style="color:blue;" | 24 || style="color:blue;" | $900 || style="color:blue;" | $990 || rowspan="2" style="color:blue;" | Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| style="color:blue;" | HTL || style="color:blue;" | 96 || style="color:blue;" | $1,800 || style="color:blue;" | $1,980
|-
| Tagmentation   NEXTERA-XT || rowspan="2" | STD || rowspan="2" | per Sample || $105 || $115 || rowspan="2" | Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation   NEXTERA-FLEX || $157 || $173
|-
| rowspan="2" | NEXTERA-XT OR FLEX || style="color:blue;" | HTL || style="color:blue;" | ''24'' || style="color:blue;" | $900 || style="color:blue;" | $990 || rowspan="2" style="color:blue;" | Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| style="color:blue;" | HTL || style="color:blue;" | ''96'' || style="color:blue;" | $1,800 || style="color:blue;" | $1,980
|-
| rowspan="2" | 16S Amplicon || style="color:blue;" | HTL || style="color:blue;" | 48 || style="color:blue;" | $1,900 || style="color:blue;" | $2,090 || rowspan="2" style="color:blue;" | Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| style="color:blue;" | HTL || style="color:blue;" | add'l 48 || style="color:blue;" | $650 || style="color:blue;" | $715
|-
| rowspan="2" | Other Amplicon || style="color:blue;" | HTL || style="color:blue;" | 48 || style="color:blue;" | $1,600 || style="color:blue;" | $1,760 || rowspan="2" style="color:blue;" | Includes normalization, enrichment, pooling and quality control (SYBR and FA).   Samples *must* be submitted amplified with appropriate linkers.
|-
| style="color:blue;" | HTL || style="color:blue;" | add'l 48 || style="color:blue;" | $630 || style="color:blue;" | $693
|-
| colspan="6" style="font-weight:bold; text-align:center;" | '''ADDITIONAL FEES'''   Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $60 || $66 ||
|-
| Sample Cleanup || || per sample || $5 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10 || $11 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! colspan="3" | ILLUMINA Sample Preparation !! colspan="3" style="color:blue;" |  
|-
| colspan="3" style="font-weight:bold; text-align:center;" | '''STANDARD RNA'''   Prices for individual samples. Repreps will typically be attempted on failed samples without charge. || colspan="3" style="font-weight:bold; text-align:center; color:blue;" | '''HIGH THROUGHPUT RNA'''   Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| rowspan="3" | polyA based isolation   NEB UltraII RNAseq || '''STD''' || per sample || $157 || $173 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| style="color:blue;" | '''HTL''' (NEB only) || style="color:blue;" | ''24'' || style="color:blue;" | $1,500 || style="color:blue;" | $1,650 || rowspan="2" style="color:blue;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| style="color:blue;" | '''HTL''' (NEB only) || style="color:blue;" | ''96'' || style="color:blue;" | $3,000 || style="color:blue;" | $3,300
|-
| rowspan="2" | High Throughput 3' Digital Gene Expression (HT3DGE) || style="color:blue;" | '''HTL''' || style="color:blue;" | ''24'' || style="color:blue;" | $1,050 || style="color:blue;" | $1,155 || rowspan="2" style="color:blue;" | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| style="color:blue;" | '''HTL''' || style="color:blue;" | ''96'' || style="color:blue;" | $2,100 || style="color:blue;" | $2,310
|-
| rowspan="3" | Total RNA sequencing   NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| style="color:blue;" | '''HTL''' (NEB only, covaris RNA fragmentation) || style="color:blue;" | ''24'' || style="color:blue;" | $2,500 || style="color:blue;" | $2,750 || rowspan="2" style="color:blue;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| style="color:blue;" | '''HTL''' (NEB only, covaris RNA fragmentation) || style="color:blue;" | ''96'' || style="color:blue;" | $5,000 || style="color:blue;" | $5,500
|-
| rowspan="3" | Low Input polyA+   Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262 || $289 || rowspan="3" | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| style="color:blue;" | '''HTL''' v2 || style="color:blue;" | ''24'' || style="color:blue;" | $2,000 || style="color:blue;" | $2,200
|-
| style="color:blue;" | '''HTL''' v2 || style="color:blue;" | ''96'' || style="color:blue;" | $3,200 || style="color:blue;" | $3,520
|-
| rowspan="3" | Low Input Mammalian Total   Clontech ZapR Kit || STD || per sample || $210 || $231 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| style="color:blue;" | '''HTL''' (covaris RNA fragmentation) || style="color:blue;" | ''24'' || style="color:blue;" | $3,000 || style="color:blue;" | $3,300 || rowspan="2" style="color:blue;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| style="color:blue;" | '''HTL''' (covaris RNA fragmentation) || style="color:blue;" | ''96'' || style="color:blue;" | $5,000 || style="color:blue;" | $5,500
|-
| Small RNA || STD || per sample || $289 || $318 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" style="font-weight:bold; text-align:center;" | '''ADDITIONAL FEES'''   Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $60 || $66 ||
|-
| Sample Cleanup || || per sample || $5 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10 || $11 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit
|-
| Standard Ligation || LSK || $160 || $176 || per sample/pool
|-
| Native Barcoding   (ligation indexing) || - || $50 || $55 || rowspan="5" | per sample
|-
| SMARTseq -> Ligation || Cv4+LSK || $300 || $330
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150 || $165
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262 || $289
|-
| Direct RNA || RNA004 || $160 || $176
|}

{| class="wikitable"
|-
! PacBio Sample Preparation !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit
|-
| rowspan="2" | Basic Library || $315 || $346 || Per Library
|-
| $157 || $173 || Per add'l Sample
|-
| rowspan="2" | Genomic Library || $472 || $520 || Per Library
|-
| $235 || $258 || Per add'l Sample
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| DNA || 96 || $700 || $770
|-
| RNA || 96 || $800 || $880
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300 || $330
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000 || $1,100
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

Please email biomicro@mit.edu two weeks prior to desired submission date so we will have reagents ready for you.

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| rowspan="2" | 5' RNA   3' RNA || setup || $1,015 || $1,116.50 || charge per submission
|-
| per sample || $1,750 || $1,925 || Includes QC of cDNA and creation of Short Read Library
|-
| rowspan="2" | 5' RNA   3' RNA - on chip multiplexing || setup || $1,150 || $1,265 || charge per submission
|-
| per 4 samples || $2,305 || $2,535 || Includes QC of cDNA and creation of Short Read Library
|-
| rowspan="2" | Fixed RNA (Flex v2)   probe based. human/mouse only || setup || $2,700 || $2,970 || charge per submission - up to 48 samples
|-
| per sample (1-plex) || $270 || $297 || Includes QC of cDNA and creation of Short Read Library
|-
| rowspan="2" | Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc // per amplicon) || setup || $470 || $517 || additional prep beyond DGE
|-
| per sample || $45 || $50 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| rowspan="2" | ATACseq || setup || $880 || $968 || charge per submission
|-
| per sample || $1,675 || $1,843 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| rowspan="2" | MULTI || setup || $1,800 || $1,980 || charge per submission
|-
| per sample || $3,100 || $3,410 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || || $200 || $220 || <24h notice
|}

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| HD: polyA or FFPE (hs/mm only) || 1 slide / 2 tiles || $7,850 || $8,596 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $60 || $66 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $40 || $44 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Slide Only || rowspan="5" | per slide || $150 || $165 || slide: 1, 12, or 48 well
|-
| Optimization Only || $800 || $880 || used when the run fails
|-
| [https://www.elementbiosciences.com/products/aviti24/cytoprofiling Teton Defined Panel] || $7,200 || $7,920 || scroll to predefined panels
|-
| Atlas Short || $5,500 || $6,050 || for CRISPR screens / short sequencing
|-
| [https://www.elementbiosciences.com/products/aviti24/cytoprofiling Antibody Add-on] || $360 || $396 || scroll to flexible protein customization
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15 || $16.50 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80 || $88 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30 || $33 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $160 || $176 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50 || $55 ||
|-
| qPCR only (Short read quantification test) || per sample || $25 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400 || $440 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $40 || $44 ||
|-
| FFPE Curl || per curl || $60 || $66 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| rowspan="6" | DNAscript Syntax || Setup (per run) || $1,000 || $1,100 || Per run cost
|-
| 2000 nucleotide synthesis || $500 || $550 || Up to 96 samples
|-
| 8000 nucleotide synthesis || $1,500 || $1,650 || Up to 96 samples
|-
| add Biotin (max48) || $115 || $126 || max 48 per run and 2 per oligo. Couple to T only.
|-
| add Fluor || $300 || $330 ||
|-
| add quench || $100 || $110 ||
|}

== [[BioMicroCenter:RTPCR|REAL TIME PCR]] ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| Roche LightCycler 480 || $15 || per plate || [https://mit.ilabsolutions.com/schedules/261680#/schedule/ CALENDAR] *available for CORE only || Email biomicro@mit.edu for training
|-
| SYBR Green (KAPA/Roche) || $45 || per ml || || Available for MIT only
|-
| 96-well PLATES || $10 || per plate || || Available for MIT only
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $20 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $75 || per gel (5 lanes) || TBA ||
|-
| Training || $25 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $18 || per hour || [https://mit.ilabsolutions.com/schedules/262715#/schedule/ CALENDAR] || Email biomicro@mit.edu for training
|-
| colspan="5" | '''ROBOTICS'''
|-
| Tecan EVO 150 || $75 || per 1h block - based on scheduled time. || [https://mit.ilabsolutions.com/schedules/262347#/schedule/ CALENDAR] ||
|-
| non-filter tips || $8 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 || per box || ||
|-
| Training || $65 || per hour || Email biomicro@mit.edu ||
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90 || $125 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100 || $110 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100 || $300 ||
|-
| Tape Archive / Recovery || per tape || $60 || $70 ||
|-
| Informatics Training Classes || per session || $50 || $100 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-27T17:12:46Z

Hilo1: /* OTHER LAB SERVICES */

'''PRICING UPDATE 7/1/2025'''

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850 || $2,000 || rowspan="2" | Per Lane || rowspan="5" | Through collaboration with nearby academic shared resources
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600 || $2,860
|-
| NovaSeqX 10B 300nt^^ || $12,160 || $13,376 || rowspan="3" | Per Flowcell
|-
| NovaSeqX 25B 300nt^^ || $16,200 || $17,820
|-
| NovaSeqX 25B 100nt^^ || $5,750 || $6,325
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| rowspan="2" | 75 PE || Full flowcell (2 lane, 800m read) || $1,900 || $2,090 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| One lane (~400m read) || $1,000 || $1,100 || per lane
|-
| rowspan="2" | 150 PE || Full flowcell (2 lane, ~800m read) || $2,650 || $2,915 || per flowcell
|-
| One lane (~400m read) || $1,375 || $1,513 || per lane
|-
| 300 PE || Full flowcell (~200m reads) || $3,500 || $3,850 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100m reads) || $300 || $330 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell (~400m reads)* || $1,800 || $1,980 || per flowcell
|-
| 150 PE: F3 Lane (~100m reads) || $450 || $495 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

{| class="wikitable"
|-
! Length !! Reads !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 300nt || rowspan="2" | 5M || $715 || $786.50 || rowspan="9" | per kit || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || $1,040 || $1,144
|-
| 100nt || rowspan="4" | 25M || $925 || $1,017.50
|-
| 300nt || $1,334 || $1,467.40
|-
| 600nt || $1,570 || $1,727
|-
| '''1000nt''' || $2,590 || $2,849
|-
| 100nt || rowspan="2" | 100M || $1,540 || $1,694
|-
| 300nt || $2,125 || $2,337.50
|-
| 600nt || 50M || $2,125 || $2,337.50
|}

==== MiSeq i100 Walkup ====
Training and lab storage on BMC servers required. Run must be scheduled in the [https://mit.ilabsolutions.com/schedules/555535#/schedule/ iLabs calendar.]

{| class="wikitable"
|-
! Length !! Reads !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! RunTime !! Notes
|-
| Machine usage || — || $120 || $132 || per run || — || training and lab storage on BMC servers required.
|-
| 300nt || rowspan="2" | 5M || $381 || $419.10 || rowspan="9" | per kit || 8h || rowspan="9" |
|-
| 600nt || $665 || $731.50 || 16h
|-
| 100nt || rowspan="4" | 25M || $565 || $621.50 || 5h
|-
| 300nt || $920 || $1,012 || 8h
|-
| 600nt || $1,125 || $1,238 || 16h
|-
| 1000nt || $1,921 || $2,113.10 || 24h
|-
| 100nt || rowspan="2" | 100M || $1,130 || $1,243 || 5h
|-
| 300nt || $1,638.50 || $1,802.35 || 8h
|-
| 600nt || 50M || $1,638.50 || $1,802.35 || 16h
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! Length !! Reads !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 70nt || 15M || $1,320 || $1,452 || rowspan="6" | per kit || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt || 25M || $1,430 || $1,573
|-
| 300nt || 15M || $1,595 || $1,754.50
|-
| 500nt || 15M || $1,760 || $1,936
|-
| 600nt || 25M || $2,200 || $2,420
|-
| 500nt || 1M  '''NANO''' || $770 || $847
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only -only one QC is included per sequencing lane. || $35 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate(short read) || $400 || $440 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200 || $1,320 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| SMRT Cell || $2,900 || $3,190 || per SMRT Cell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! colspan="3" | ILLUMINA Sample Preparation !! colspan="3" style="color:blue;" |  
|-
| colspan="3" style="font-weight:bold; text-align:center;" | '''STANDARD THROUGHPUT DNA'''   Prices for individual samples. Repreps will typically be attempted on failed samples without charge. || colspan="3" style="font-weight:bold; text-align:center; color:blue;" | '''HIGH THROUGHPUT DNA'''   Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| rowspan="3" | LIGATION BASED NEB UltraII || STD || per sample || $105 || $115 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| style="color:blue;" | HTL || style="color:blue;" | 24 || style="color:blue;" | $900 || style="color:blue;" | $990 || rowspan="2" style="color:blue;" | Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| style="color:blue;" | HTL || style="color:blue;" | 96 || style="color:blue;" | $1,800 || style="color:blue;" | $1,980
|-
| Tagmentation   NEXTERA-XT || rowspan="2" | STD || rowspan="2" | per Sample || $105 || $115 || rowspan="2" | Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation   NEXTERA-FLEX || $157 || $173
|-
| rowspan="2" | NEXTERA-XT OR FLEX || style="color:blue;" | HTL || style="color:blue;" | ''24'' || style="color:blue;" | $900 || style="color:blue;" | $990 || rowspan="2" style="color:blue;" | Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| style="color:blue;" | HTL || style="color:blue;" | ''96'' || style="color:blue;" | $1,800 || style="color:blue;" | $1,980
|-
| rowspan="2" | 16S Amplicon || style="color:blue;" | HTL || style="color:blue;" | 48 || style="color:blue;" | $1,900 || style="color:blue;" | $2,090 || rowspan="2" style="color:blue;" | Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| style="color:blue;" | HTL || style="color:blue;" | add'l 48 || style="color:blue;" | $650 || style="color:blue;" | $715
|-
| rowspan="2" | Other Amplicon || style="color:blue;" | HTL || style="color:blue;" | 48 || style="color:blue;" | $1,600 || style="color:blue;" | $1,760 || rowspan="2" style="color:blue;" | Includes normalization, enrichment, pooling and quality control (SYBR and FA).   Samples *must* be submitted amplified with appropriate linkers.
|-
| style="color:blue;" | HTL || style="color:blue;" | add'l 48 || style="color:blue;" | $630 || style="color:blue;" | $693
|-
| colspan="6" style="font-weight:bold; text-align:center;" | '''ADDITIONAL FEES'''   Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $60 || $66 ||
|-
| Sample Cleanup || || per sample || $5 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10 || $11 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! colspan="3" | ILLUMINA Sample Preparation !! colspan="3" style="color:blue;" |  
|-
| colspan="3" style="font-weight:bold; text-align:center;" | '''STANDARD RNA'''   Prices for individual samples. Repreps will typically be attempted on failed samples without charge. || colspan="3" style="font-weight:bold; text-align:center; color:blue;" | '''HIGH THROUGHPUT RNA'''   Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| rowspan="3" | polyA based isolation   NEB UltraII RNAseq || '''STD''' || per sample || $157 || $173 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| style="color:blue;" | '''HTL''' (NEB only) || style="color:blue;" | ''24'' || style="color:blue;" | $1,500 || style="color:blue;" | $1,650 || rowspan="2" style="color:blue;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| style="color:blue;" | '''HTL''' (NEB only) || style="color:blue;" | ''96'' || style="color:blue;" | $3,000 || style="color:blue;" | $3,300
|-
| rowspan="2" | High Throughput 3' Digital Gene Expression (HT3DGE) || style="color:blue;" | '''HTL''' || style="color:blue;" | ''24'' || style="color:blue;" | $1,050 || style="color:blue;" | $1,155 || rowspan="2" style="color:blue;" | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| style="color:blue;" | '''HTL''' || style="color:blue;" | ''96'' || style="color:blue;" | $2,100 || style="color:blue;" | $2,310
|-
| rowspan="3" | Total RNA sequencing   NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| style="color:blue;" | '''HTL''' (NEB only, covaris RNA fragmentation) || style="color:blue;" | ''24'' || style="color:blue;" | $2,500 || style="color:blue;" | $2,750 || rowspan="2" style="color:blue;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| style="color:blue;" | '''HTL''' (NEB only, covaris RNA fragmentation) || style="color:blue;" | ''96'' || style="color:blue;" | $5,000 || style="color:blue;" | $5,500
|-
| rowspan="3" | Low Input polyA+   Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262 || $289 || rowspan="3" | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| style="color:blue;" | '''HTL''' v2 || style="color:blue;" | ''24'' || style="color:blue;" | $2,000 || style="color:blue;" | $2,200
|-
| style="color:blue;" | '''HTL''' v2 || style="color:blue;" | ''96'' || style="color:blue;" | $3,200 || style="color:blue;" | $3,520
|-
| rowspan="3" | Low Input Mammalian Total   Clontech ZapR Kit || STD || per sample || $210 || $231 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| style="color:blue;" | '''HTL''' (covaris RNA fragmentation) || style="color:blue;" | ''24'' || style="color:blue;" | $3,000 || style="color:blue;" | $3,300 || rowspan="2" style="color:blue;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| style="color:blue;" | '''HTL''' (covaris RNA fragmentation) || style="color:blue;" | ''96'' || style="color:blue;" | $5,000 || style="color:blue;" | $5,500
|-
| Small RNA || STD || per sample || $289 || $318 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" style="font-weight:bold; text-align:center;" | '''ADDITIONAL FEES'''   Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $60 || $66 ||
|-
| Sample Cleanup || || per sample || $5 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10 || $11 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit
|-
| Standard Ligation || LSK || $160 || $176 || per sample/pool
|-
| Native Barcoding   (ligation indexing) || - || $50 || $55 || rowspan="5" | per sample
|-
| SMARTseq -> Ligation || Cv4+LSK || $300 || $330
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150 || $165
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262 || $289
|-
| Direct RNA || RNA004 || $160 || $176
|}

{| class="wikitable"
|-
! PacBio Sample Preparation !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit
|-
| rowspan="2" | Basic Library || $315 || $346 || Per Library
|-
| $157 || $173 || Per add'l Sample
|-
| rowspan="2" | Genomic Library || $472 || $520 || Per Library
|-
| $235 || $258 || Per add'l Sample
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| DNA || 96 || $700 || $770
|-
| RNA || 96 || $800 || $880
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300 || $330
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000 || $1,100
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

Please email biomicro@mit.edu two weeks prior to desired submission date so we will have reagents ready for you.

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| rowspan="2" | 5' RNA   3' RNA || setup || $1,015 || $1,116.50 || charge per submission
|-
| per sample || $1,750 || $1,925 || Includes QC of cDNA and creation of Short Read Library
|-
| rowspan="2" | 5' RNA   3' RNA - on chip multiplexing || setup || $1,150 || $1,265 || charge per submission
|-
| per 4 samples || $2,305 || $2,535 || Includes QC of cDNA and creation of Short Read Library
|-
| rowspan="2" | Fixed RNA (Flex v2)   probe based. human/mouse only || setup || $2,700 || $2,970 || charge per submission - up to 48 samples
|-
| per sample (1-plex) || $270 || $297 || Includes QC of cDNA and creation of Short Read Library
|-
| rowspan="2" | Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc // per amplicon) || setup || $470 || $517 || additional prep beyond DGE
|-
| per sample || $45 || $50 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| rowspan="2" | ATACseq || setup || $880 || $968 || charge per submission
|-
| per sample || $1,675 || $1,843 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| rowspan="2" | MULTI || setup || $1,800 || $1,980 || charge per submission
|-
| per sample || $3,100 || $3,410 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || || $200 || $220 || <24h notice
|}

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| HD: polyA or FFPE (hs/mm only) || 1 slide / 2 tiles || $7,850 || $8,596 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $60 || $66 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $40 || $44 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Slide Only || rowspan="5" | per slide || $150 || $165 || slide: 1, 12, or 48 well
|-
| Optimization Only || $800 || $880 || used when the run fails
|-
| [https://www.elementbiosciences.com/products/aviti24/cytoprofiling Teton Defined Panel] || $7,200 || $7,920 || scroll to predefined panels
|-
| Atlas Short || $5,500 || $6,050 || for CRISPR screens / short sequencing
|-
| [https://www.elementbiosciences.com/products/aviti24/cytoprofiling Antibody Add-on] || $360 || $396 || scroll to flexible protein customization
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15 || $16.50 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80 || $88 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30 || $33 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $160 || $176 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50 || $55 ||
|-
| qPCR only (Short read quantification test) || per sample || $25 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400 || $440 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $40 || $44 ||
|-
| FFPE Curl || per curl || $60 || $66 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| rowspan="6" | DNAscript Syntax || Setup (per run) || $1,000 || $1,100 || Per run cost
|-
| 2000 nucleotide synthesis || $500 || $550 || Up to 96 samples
|-
| 8000 nucleotide synthesis || $1,500 || $1,650 || Up to 96 samples
|-
| add Biotin (max48) || $115 || $126 || max 48 per run and 2 per oligo. Couple to T only.
|-
| add Fluor || $300 || $330 ||
|-
| add quench || $100 || $110 ||
|}

== [[BioMicroCenter:RTPCR|REAL TIME PCR]] ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| Roche LightCycler 480 || $15 || per plate || [https://mit.ilabsolutions.com/schedules/261680#/schedule/ CALENDAR] *available for CORE only || Email biomicro@mit.edu for training
|-
| SYBR Green (KAPA/Roche) || $45 || per ml || || Available for MIT only
|-
| 96-well PLATES || $10 || per plate || || Available for MIT only
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $20 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $75 || per gel (5 lanes) || TBA ||
|-
| Training || $25 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $18 || per hour || [https://mit.ilabsolutions.com/schedules/262715#/schedule/ CALENDAR] || Email biomicro@mit.edu for training
|-
| colspan="5" | '''ROBOTICS'''
|-
| Tecan EVO 150 || $75 || per 1h block - based on scheduled time. || [https://mit.ilabsolutions.com/schedules/262347#/schedule/ CALENDAR] ||
|-
| non-filter tips || $8 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 || per box || ||
|-
| Training || $65 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]] ||
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90 || $125 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100 || $110 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100 || $300 ||
|-
| Tape Archive / Recovery || per tape || $60 || $70 ||
|-
| Informatics Training Classes || per session || $50 || $100 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-27T17:12:20Z

Hilo1: /* REAL TIME PCR */

'''PRICING UPDATE 7/1/2025'''

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850 || $2,000 || rowspan="2" | Per Lane || rowspan="5" | Through collaboration with nearby academic shared resources
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600 || $2,860
|-
| NovaSeqX 10B 300nt^^ || $12,160 || $13,376 || rowspan="3" | Per Flowcell
|-
| NovaSeqX 25B 300nt^^ || $16,200 || $17,820
|-
| NovaSeqX 25B 100nt^^ || $5,750 || $6,325
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| rowspan="2" | 75 PE || Full flowcell (2 lane, 800m read) || $1,900 || $2,090 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| One lane (~400m read) || $1,000 || $1,100 || per lane
|-
| rowspan="2" | 150 PE || Full flowcell (2 lane, ~800m read) || $2,650 || $2,915 || per flowcell
|-
| One lane (~400m read) || $1,375 || $1,513 || per lane
|-
| 300 PE || Full flowcell (~200m reads) || $3,500 || $3,850 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100m reads) || $300 || $330 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell (~400m reads)* || $1,800 || $1,980 || per flowcell
|-
| 150 PE: F3 Lane (~100m reads) || $450 || $495 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

{| class="wikitable"
|-
! Length !! Reads !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 300nt || rowspan="2" | 5M || $715 || $786.50 || rowspan="9" | per kit || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || $1,040 || $1,144
|-
| 100nt || rowspan="4" | 25M || $925 || $1,017.50
|-
| 300nt || $1,334 || $1,467.40
|-
| 600nt || $1,570 || $1,727
|-
| '''1000nt''' || $2,590 || $2,849
|-
| 100nt || rowspan="2" | 100M || $1,540 || $1,694
|-
| 300nt || $2,125 || $2,337.50
|-
| 600nt || 50M || $2,125 || $2,337.50
|}

==== MiSeq i100 Walkup ====
Training and lab storage on BMC servers required. Run must be scheduled in the [https://mit.ilabsolutions.com/schedules/555535#/schedule/ iLabs calendar.]

{| class="wikitable"
|-
! Length !! Reads !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! RunTime !! Notes
|-
| Machine usage || — || $120 || $132 || per run || — || training and lab storage on BMC servers required.
|-
| 300nt || rowspan="2" | 5M || $381 || $419.10 || rowspan="9" | per kit || 8h || rowspan="9" |
|-
| 600nt || $665 || $731.50 || 16h
|-
| 100nt || rowspan="4" | 25M || $565 || $621.50 || 5h
|-
| 300nt || $920 || $1,012 || 8h
|-
| 600nt || $1,125 || $1,238 || 16h
|-
| 1000nt || $1,921 || $2,113.10 || 24h
|-
| 100nt || rowspan="2" | 100M || $1,130 || $1,243 || 5h
|-
| 300nt || $1,638.50 || $1,802.35 || 8h
|-
| 600nt || 50M || $1,638.50 || $1,802.35 || 16h
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! Length !! Reads !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 70nt || 15M || $1,320 || $1,452 || rowspan="6" | per kit || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt || 25M || $1,430 || $1,573
|-
| 300nt || 15M || $1,595 || $1,754.50
|-
| 500nt || 15M || $1,760 || $1,936
|-
| 600nt || 25M || $2,200 || $2,420
|-
| 500nt || 1M  '''NANO''' || $770 || $847
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only -only one QC is included per sequencing lane. || $35 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate(short read) || $400 || $440 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200 || $1,320 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| SMRT Cell || $2,900 || $3,190 || per SMRT Cell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! colspan="3" | ILLUMINA Sample Preparation !! colspan="3" style="color:blue;" |  
|-
| colspan="3" style="font-weight:bold; text-align:center;" | '''STANDARD THROUGHPUT DNA'''   Prices for individual samples. Repreps will typically be attempted on failed samples without charge. || colspan="3" style="font-weight:bold; text-align:center; color:blue;" | '''HIGH THROUGHPUT DNA'''   Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| rowspan="3" | LIGATION BASED NEB UltraII || STD || per sample || $105 || $115 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| style="color:blue;" | HTL || style="color:blue;" | 24 || style="color:blue;" | $900 || style="color:blue;" | $990 || rowspan="2" style="color:blue;" | Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| style="color:blue;" | HTL || style="color:blue;" | 96 || style="color:blue;" | $1,800 || style="color:blue;" | $1,980
|-
| Tagmentation   NEXTERA-XT || rowspan="2" | STD || rowspan="2" | per Sample || $105 || $115 || rowspan="2" | Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation   NEXTERA-FLEX || $157 || $173
|-
| rowspan="2" | NEXTERA-XT OR FLEX || style="color:blue;" | HTL || style="color:blue;" | ''24'' || style="color:blue;" | $900 || style="color:blue;" | $990 || rowspan="2" style="color:blue;" | Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| style="color:blue;" | HTL || style="color:blue;" | ''96'' || style="color:blue;" | $1,800 || style="color:blue;" | $1,980
|-
| rowspan="2" | 16S Amplicon || style="color:blue;" | HTL || style="color:blue;" | 48 || style="color:blue;" | $1,900 || style="color:blue;" | $2,090 || rowspan="2" style="color:blue;" | Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| style="color:blue;" | HTL || style="color:blue;" | add'l 48 || style="color:blue;" | $650 || style="color:blue;" | $715
|-
| rowspan="2" | Other Amplicon || style="color:blue;" | HTL || style="color:blue;" | 48 || style="color:blue;" | $1,600 || style="color:blue;" | $1,760 || rowspan="2" style="color:blue;" | Includes normalization, enrichment, pooling and quality control (SYBR and FA).   Samples *must* be submitted amplified with appropriate linkers.
|-
| style="color:blue;" | HTL || style="color:blue;" | add'l 48 || style="color:blue;" | $630 || style="color:blue;" | $693
|-
| colspan="6" style="font-weight:bold; text-align:center;" | '''ADDITIONAL FEES'''   Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $60 || $66 ||
|-
| Sample Cleanup || || per sample || $5 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10 || $11 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! colspan="3" | ILLUMINA Sample Preparation !! colspan="3" style="color:blue;" |  
|-
| colspan="3" style="font-weight:bold; text-align:center;" | '''STANDARD RNA'''   Prices for individual samples. Repreps will typically be attempted on failed samples without charge. || colspan="3" style="font-weight:bold; text-align:center; color:blue;" | '''HIGH THROUGHPUT RNA'''   Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| rowspan="3" | polyA based isolation   NEB UltraII RNAseq || '''STD''' || per sample || $157 || $173 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| style="color:blue;" | '''HTL''' (NEB only) || style="color:blue;" | ''24'' || style="color:blue;" | $1,500 || style="color:blue;" | $1,650 || rowspan="2" style="color:blue;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| style="color:blue;" | '''HTL''' (NEB only) || style="color:blue;" | ''96'' || style="color:blue;" | $3,000 || style="color:blue;" | $3,300
|-
| rowspan="2" | High Throughput 3' Digital Gene Expression (HT3DGE) || style="color:blue;" | '''HTL''' || style="color:blue;" | ''24'' || style="color:blue;" | $1,050 || style="color:blue;" | $1,155 || rowspan="2" style="color:blue;" | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| style="color:blue;" | '''HTL''' || style="color:blue;" | ''96'' || style="color:blue;" | $2,100 || style="color:blue;" | $2,310
|-
| rowspan="3" | Total RNA sequencing   NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| style="color:blue;" | '''HTL''' (NEB only, covaris RNA fragmentation) || style="color:blue;" | ''24'' || style="color:blue;" | $2,500 || style="color:blue;" | $2,750 || rowspan="2" style="color:blue;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| style="color:blue;" | '''HTL''' (NEB only, covaris RNA fragmentation) || style="color:blue;" | ''96'' || style="color:blue;" | $5,000 || style="color:blue;" | $5,500
|-
| rowspan="3" | Low Input polyA+   Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262 || $289 || rowspan="3" | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| style="color:blue;" | '''HTL''' v2 || style="color:blue;" | ''24'' || style="color:blue;" | $2,000 || style="color:blue;" | $2,200
|-
| style="color:blue;" | '''HTL''' v2 || style="color:blue;" | ''96'' || style="color:blue;" | $3,200 || style="color:blue;" | $3,520
|-
| rowspan="3" | Low Input Mammalian Total   Clontech ZapR Kit || STD || per sample || $210 || $231 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| style="color:blue;" | '''HTL''' (covaris RNA fragmentation) || style="color:blue;" | ''24'' || style="color:blue;" | $3,000 || style="color:blue;" | $3,300 || rowspan="2" style="color:blue;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| style="color:blue;" | '''HTL''' (covaris RNA fragmentation) || style="color:blue;" | ''96'' || style="color:blue;" | $5,000 || style="color:blue;" | $5,500
|-
| Small RNA || STD || per sample || $289 || $318 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" style="font-weight:bold; text-align:center;" | '''ADDITIONAL FEES'''   Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $60 || $66 ||
|-
| Sample Cleanup || || per sample || $5 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10 || $11 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit
|-
| Standard Ligation || LSK || $160 || $176 || per sample/pool
|-
| Native Barcoding   (ligation indexing) || - || $50 || $55 || rowspan="5" | per sample
|-
| SMARTseq -> Ligation || Cv4+LSK || $300 || $330
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150 || $165
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262 || $289
|-
| Direct RNA || RNA004 || $160 || $176
|}

{| class="wikitable"
|-
! PacBio Sample Preparation !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit
|-
| rowspan="2" | Basic Library || $315 || $346 || Per Library
|-
| $157 || $173 || Per add'l Sample
|-
| rowspan="2" | Genomic Library || $472 || $520 || Per Library
|-
| $235 || $258 || Per add'l Sample
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| DNA || 96 || $700 || $770
|-
| RNA || 96 || $800 || $880
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300 || $330
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000 || $1,100
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

Please email biomicro@mit.edu two weeks prior to desired submission date so we will have reagents ready for you.

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| rowspan="2" | 5' RNA   3' RNA || setup || $1,015 || $1,116.50 || charge per submission
|-
| per sample || $1,750 || $1,925 || Includes QC of cDNA and creation of Short Read Library
|-
| rowspan="2" | 5' RNA   3' RNA - on chip multiplexing || setup || $1,150 || $1,265 || charge per submission
|-
| per 4 samples || $2,305 || $2,535 || Includes QC of cDNA and creation of Short Read Library
|-
| rowspan="2" | Fixed RNA (Flex v2)   probe based. human/mouse only || setup || $2,700 || $2,970 || charge per submission - up to 48 samples
|-
| per sample (1-plex) || $270 || $297 || Includes QC of cDNA and creation of Short Read Library
|-
| rowspan="2" | Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc // per amplicon) || setup || $470 || $517 || additional prep beyond DGE
|-
| per sample || $45 || $50 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| rowspan="2" | ATACseq || setup || $880 || $968 || charge per submission
|-
| per sample || $1,675 || $1,843 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| rowspan="2" | MULTI || setup || $1,800 || $1,980 || charge per submission
|-
| per sample || $3,100 || $3,410 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || || $200 || $220 || <24h notice
|}

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| HD: polyA or FFPE (hs/mm only) || 1 slide / 2 tiles || $7,850 || $8,596 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $60 || $66 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $40 || $44 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Slide Only || rowspan="5" | per slide || $150 || $165 || slide: 1, 12, or 48 well
|-
| Optimization Only || $800 || $880 || used when the run fails
|-
| [https://www.elementbiosciences.com/products/aviti24/cytoprofiling Teton Defined Panel] || $7,200 || $7,920 || scroll to predefined panels
|-
| Atlas Short || $5,500 || $6,050 || for CRISPR screens / short sequencing
|-
| [https://www.elementbiosciences.com/products/aviti24/cytoprofiling Antibody Add-on] || $360 || $396 || scroll to flexible protein customization
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15 || $16.50 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80 || $88 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30 || $33 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $160 || $176 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50 || $55 ||
|-
| qPCR only (Short read quantification test) || per sample || $25 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400 || $440 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $40 || $44 ||
|-
| FFPE Curl || per curl || $60 || $66 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| rowspan="6" | DNAscript Syntax || Setup (per run) || $1,000 || $1,100 || Per run cost
|-
| 2000 nucleotide synthesis || $500 || $550 || Up to 96 samples
|-
| 8000 nucleotide synthesis || $1,500 || $1,650 || Up to 96 samples
|-
| add Biotin (max48) || $115 || $126 || max 48 per run and 2 per oligo. Couple to T only.
|-
| add Fluor || $300 || $330 ||
|-
| add quench || $100 || $110 ||
|}

== [[BioMicroCenter:RTPCR|REAL TIME PCR]] ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| Roche LightCycler 480 || $15 || per plate || [https://mit.ilabsolutions.com/schedules/261680#/schedule/ CALENDAR] *available for CORE only || Email biomicro@mit.edu for training
|-
| SYBR Green (KAPA/Roche) || $45 || per ml || || Available for MIT only
|-
| 96-well PLATES || $10 || per plate || || Available for MIT only
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $20 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $75 || per gel (5 lanes) || TBA ||
|-
| Training || $25 || per session || TBA || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $18 || per hour || [https://mit.ilabsolutions.com/schedules/262715#/schedule/ CALENDAR] || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="5" | '''ROBOTICS'''
|-
| Tecan EVO 150 || $75 || per 1h block - based on scheduled time. || [https://mit.ilabsolutions.com/schedules/262347#/schedule/ CALENDAR] ||
|-
| non-filter tips || $8 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 || per box || ||
|-
| Training || $65 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]] ||
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90 || $125 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100 || $110 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100 || $300 ||
|-
| Tape Archive / Recovery || per tape || $60 || $70 ||
|-
| Informatics Training Classes || per session || $50 || $100 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-27T17:11:41Z

Hilo1: /* CHEMAGIC360 */

'''PRICING UPDATE 7/1/2025'''

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850 || $2,000 || rowspan="2" | Per Lane || rowspan="5" | Through collaboration with nearby academic shared resources
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600 || $2,860
|-
| NovaSeqX 10B 300nt^^ || $12,160 || $13,376 || rowspan="3" | Per Flowcell
|-
| NovaSeqX 25B 300nt^^ || $16,200 || $17,820
|-
| NovaSeqX 25B 100nt^^ || $5,750 || $6,325
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| rowspan="2" | 75 PE || Full flowcell (2 lane, 800m read) || $1,900 || $2,090 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| One lane (~400m read) || $1,000 || $1,100 || per lane
|-
| rowspan="2" | 150 PE || Full flowcell (2 lane, ~800m read) || $2,650 || $2,915 || per flowcell
|-
| One lane (~400m read) || $1,375 || $1,513 || per lane
|-
| 300 PE || Full flowcell (~200m reads) || $3,500 || $3,850 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100m reads) || $300 || $330 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell (~400m reads)* || $1,800 || $1,980 || per flowcell
|-
| 150 PE: F3 Lane (~100m reads) || $450 || $495 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

{| class="wikitable"
|-
! Length !! Reads !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 300nt || rowspan="2" | 5M || $715 || $786.50 || rowspan="9" | per kit || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || $1,040 || $1,144
|-
| 100nt || rowspan="4" | 25M || $925 || $1,017.50
|-
| 300nt || $1,334 || $1,467.40
|-
| 600nt || $1,570 || $1,727
|-
| '''1000nt''' || $2,590 || $2,849
|-
| 100nt || rowspan="2" | 100M || $1,540 || $1,694
|-
| 300nt || $2,125 || $2,337.50
|-
| 600nt || 50M || $2,125 || $2,337.50
|}

==== MiSeq i100 Walkup ====
Training and lab storage on BMC servers required. Run must be scheduled in the [https://mit.ilabsolutions.com/schedules/555535#/schedule/ iLabs calendar.]

{| class="wikitable"
|-
! Length !! Reads !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! RunTime !! Notes
|-
| Machine usage || — || $120 || $132 || per run || — || training and lab storage on BMC servers required.
|-
| 300nt || rowspan="2" | 5M || $381 || $419.10 || rowspan="9" | per kit || 8h || rowspan="9" |
|-
| 600nt || $665 || $731.50 || 16h
|-
| 100nt || rowspan="4" | 25M || $565 || $621.50 || 5h
|-
| 300nt || $920 || $1,012 || 8h
|-
| 600nt || $1,125 || $1,238 || 16h
|-
| 1000nt || $1,921 || $2,113.10 || 24h
|-
| 100nt || rowspan="2" | 100M || $1,130 || $1,243 || 5h
|-
| 300nt || $1,638.50 || $1,802.35 || 8h
|-
| 600nt || 50M || $1,638.50 || $1,802.35 || 16h
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! Length !! Reads !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 70nt || 15M || $1,320 || $1,452 || rowspan="6" | per kit || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt || 25M || $1,430 || $1,573
|-
| 300nt || 15M || $1,595 || $1,754.50
|-
| 500nt || 15M || $1,760 || $1,936
|-
| 600nt || 25M || $2,200 || $2,420
|-
| 500nt || 1M  '''NANO''' || $770 || $847
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only -only one QC is included per sequencing lane. || $35 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate(short read) || $400 || $440 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200 || $1,320 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| SMRT Cell || $2,900 || $3,190 || per SMRT Cell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! colspan="3" | ILLUMINA Sample Preparation !! colspan="3" style="color:blue;" |  
|-
| colspan="3" style="font-weight:bold; text-align:center;" | '''STANDARD THROUGHPUT DNA'''   Prices for individual samples. Repreps will typically be attempted on failed samples without charge. || colspan="3" style="font-weight:bold; text-align:center; color:blue;" | '''HIGH THROUGHPUT DNA'''   Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| rowspan="3" | LIGATION BASED NEB UltraII || STD || per sample || $105 || $115 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| style="color:blue;" | HTL || style="color:blue;" | 24 || style="color:blue;" | $900 || style="color:blue;" | $990 || rowspan="2" style="color:blue;" | Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| style="color:blue;" | HTL || style="color:blue;" | 96 || style="color:blue;" | $1,800 || style="color:blue;" | $1,980
|-
| Tagmentation   NEXTERA-XT || rowspan="2" | STD || rowspan="2" | per Sample || $105 || $115 || rowspan="2" | Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation   NEXTERA-FLEX || $157 || $173
|-
| rowspan="2" | NEXTERA-XT OR FLEX || style="color:blue;" | HTL || style="color:blue;" | ''24'' || style="color:blue;" | $900 || style="color:blue;" | $990 || rowspan="2" style="color:blue;" | Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| style="color:blue;" | HTL || style="color:blue;" | ''96'' || style="color:blue;" | $1,800 || style="color:blue;" | $1,980
|-
| rowspan="2" | 16S Amplicon || style="color:blue;" | HTL || style="color:blue;" | 48 || style="color:blue;" | $1,900 || style="color:blue;" | $2,090 || rowspan="2" style="color:blue;" | Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| style="color:blue;" | HTL || style="color:blue;" | add'l 48 || style="color:blue;" | $650 || style="color:blue;" | $715
|-
| rowspan="2" | Other Amplicon || style="color:blue;" | HTL || style="color:blue;" | 48 || style="color:blue;" | $1,600 || style="color:blue;" | $1,760 || rowspan="2" style="color:blue;" | Includes normalization, enrichment, pooling and quality control (SYBR and FA).   Samples *must* be submitted amplified with appropriate linkers.
|-
| style="color:blue;" | HTL || style="color:blue;" | add'l 48 || style="color:blue;" | $630 || style="color:blue;" | $693
|-
| colspan="6" style="font-weight:bold; text-align:center;" | '''ADDITIONAL FEES'''   Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $60 || $66 ||
|-
| Sample Cleanup || || per sample || $5 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10 || $11 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! colspan="3" | ILLUMINA Sample Preparation !! colspan="3" style="color:blue;" |  
|-
| colspan="3" style="font-weight:bold; text-align:center;" | '''STANDARD RNA'''   Prices for individual samples. Repreps will typically be attempted on failed samples without charge. || colspan="3" style="font-weight:bold; text-align:center; color:blue;" | '''HIGH THROUGHPUT RNA'''   Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| rowspan="3" | polyA based isolation   NEB UltraII RNAseq || '''STD''' || per sample || $157 || $173 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| style="color:blue;" | '''HTL''' (NEB only) || style="color:blue;" | ''24'' || style="color:blue;" | $1,500 || style="color:blue;" | $1,650 || rowspan="2" style="color:blue;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| style="color:blue;" | '''HTL''' (NEB only) || style="color:blue;" | ''96'' || style="color:blue;" | $3,000 || style="color:blue;" | $3,300
|-
| rowspan="2" | High Throughput 3' Digital Gene Expression (HT3DGE) || style="color:blue;" | '''HTL''' || style="color:blue;" | ''24'' || style="color:blue;" | $1,050 || style="color:blue;" | $1,155 || rowspan="2" style="color:blue;" | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| style="color:blue;" | '''HTL''' || style="color:blue;" | ''96'' || style="color:blue;" | $2,100 || style="color:blue;" | $2,310
|-
| rowspan="3" | Total RNA sequencing   NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| style="color:blue;" | '''HTL''' (NEB only, covaris RNA fragmentation) || style="color:blue;" | ''24'' || style="color:blue;" | $2,500 || style="color:blue;" | $2,750 || rowspan="2" style="color:blue;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| style="color:blue;" | '''HTL''' (NEB only, covaris RNA fragmentation) || style="color:blue;" | ''96'' || style="color:blue;" | $5,000 || style="color:blue;" | $5,500
|-
| rowspan="3" | Low Input polyA+   Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262 || $289 || rowspan="3" | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| style="color:blue;" | '''HTL''' v2 || style="color:blue;" | ''24'' || style="color:blue;" | $2,000 || style="color:blue;" | $2,200
|-
| style="color:blue;" | '''HTL''' v2 || style="color:blue;" | ''96'' || style="color:blue;" | $3,200 || style="color:blue;" | $3,520
|-
| rowspan="3" | Low Input Mammalian Total   Clontech ZapR Kit || STD || per sample || $210 || $231 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| style="color:blue;" | '''HTL''' (covaris RNA fragmentation) || style="color:blue;" | ''24'' || style="color:blue;" | $3,000 || style="color:blue;" | $3,300 || rowspan="2" style="color:blue;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| style="color:blue;" | '''HTL''' (covaris RNA fragmentation) || style="color:blue;" | ''96'' || style="color:blue;" | $5,000 || style="color:blue;" | $5,500
|-
| Small RNA || STD || per sample || $289 || $318 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" style="font-weight:bold; text-align:center;" | '''ADDITIONAL FEES'''   Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $60 || $66 ||
|-
| Sample Cleanup || || per sample || $5 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10 || $11 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit
|-
| Standard Ligation || LSK || $160 || $176 || per sample/pool
|-
| Native Barcoding   (ligation indexing) || - || $50 || $55 || rowspan="5" | per sample
|-
| SMARTseq -> Ligation || Cv4+LSK || $300 || $330
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150 || $165
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262 || $289
|-
| Direct RNA || RNA004 || $160 || $176
|}

{| class="wikitable"
|-
! PacBio Sample Preparation !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit
|-
| rowspan="2" | Basic Library || $315 || $346 || Per Library
|-
| $157 || $173 || Per add'l Sample
|-
| rowspan="2" | Genomic Library || $472 || $520 || Per Library
|-
| $235 || $258 || Per add'l Sample
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| DNA || 96 || $700 || $770
|-
| RNA || 96 || $800 || $880
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300 || $330
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000 || $1,100
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

Please email biomicro@mit.edu two weeks prior to desired submission date so we will have reagents ready for you.

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| rowspan="2" | 5' RNA   3' RNA || setup || $1,015 || $1,116.50 || charge per submission
|-
| per sample || $1,750 || $1,925 || Includes QC of cDNA and creation of Short Read Library
|-
| rowspan="2" | 5' RNA   3' RNA - on chip multiplexing || setup || $1,150 || $1,265 || charge per submission
|-
| per 4 samples || $2,305 || $2,535 || Includes QC of cDNA and creation of Short Read Library
|-
| rowspan="2" | Fixed RNA (Flex v2)   probe based. human/mouse only || setup || $2,700 || $2,970 || charge per submission - up to 48 samples
|-
| per sample (1-plex) || $270 || $297 || Includes QC of cDNA and creation of Short Read Library
|-
| rowspan="2" | Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc // per amplicon) || setup || $470 || $517 || additional prep beyond DGE
|-
| per sample || $45 || $50 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| rowspan="2" | ATACseq || setup || $880 || $968 || charge per submission
|-
| per sample || $1,675 || $1,843 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| rowspan="2" | MULTI || setup || $1,800 || $1,980 || charge per submission
|-
| per sample || $3,100 || $3,410 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || || $200 || $220 || <24h notice
|}

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| HD: polyA or FFPE (hs/mm only) || 1 slide / 2 tiles || $7,850 || $8,596 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $60 || $66 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $40 || $44 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Slide Only || rowspan="5" | per slide || $150 || $165 || slide: 1, 12, or 48 well
|-
| Optimization Only || $800 || $880 || used when the run fails
|-
| [https://www.elementbiosciences.com/products/aviti24/cytoprofiling Teton Defined Panel] || $7,200 || $7,920 || scroll to predefined panels
|-
| Atlas Short || $5,500 || $6,050 || for CRISPR screens / short sequencing
|-
| [https://www.elementbiosciences.com/products/aviti24/cytoprofiling Antibody Add-on] || $360 || $396 || scroll to flexible protein customization
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15 || $16.50 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80 || $88 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30 || $33 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $160 || $176 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50 || $55 ||
|-
| qPCR only (Short read quantification test) || per sample || $25 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400 || $440 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $40 || $44 ||
|-
| FFPE Curl || per curl || $60 || $66 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| rowspan="6" | DNAscript Syntax || Setup (per run) || $1,000 || $1,100 || Per run cost
|-
| 2000 nucleotide synthesis || $500 || $550 || Up to 96 samples
|-
| 8000 nucleotide synthesis || $1,500 || $1,650 || Up to 96 samples
|-
| add Biotin (max48) || $115 || $126 || max 48 per run and 2 per oligo. Couple to T only.
|-
| add Fluor || $300 || $330 ||
|-
| add quench || $100 || $110 ||
|}

== [[BioMicroCenter:RTPCR|REAL TIME PCR]] ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| Roche LightCycler 480 || $15 || per plate || [https://mit.ilabsolutions.com/schedules/261680#/schedule/ CALENDAR] *available for CORE only || Contact [[BioMicroCenter:People|Christopher Hallee]] for training
|-
| SYBR Green (KAPA/Roche) || $45 || per ml || || Available for MIT only
|-
| 96-well PLATES || $10 || per plate || || Available for MIT only
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $20 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $75 || per gel (5 lanes) || TBA ||
|-
| Training || $25 || per session || TBA || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $18 || per hour || [https://mit.ilabsolutions.com/schedules/262715#/schedule/ CALENDAR] || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="5" | '''ROBOTICS'''
|-
| Tecan EVO 150 || $75 || per 1h block - based on scheduled time. || [https://mit.ilabsolutions.com/schedules/262347#/schedule/ CALENDAR] ||
|-
| non-filter tips || $8 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 || per box || ||
|-
| Training || $65 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]] ||
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90 || $125 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100 || $110 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100 || $300 ||
|-
| Tape Archive / Recovery || per tape || $60 || $70 ||
|-
| Informatics Training Classes || per session || $50 || $100 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-27T17:10:56Z

Hilo1: /* MiSeq classic */

'''PRICING UPDATE 7/1/2025'''

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850 || $2,000 || rowspan="2" | Per Lane || rowspan="5" | Through collaboration with nearby academic shared resources
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600 || $2,860
|-
| NovaSeqX 10B 300nt^^ || $12,160 || $13,376 || rowspan="3" | Per Flowcell
|-
| NovaSeqX 25B 300nt^^ || $16,200 || $17,820
|-
| NovaSeqX 25B 100nt^^ || $5,750 || $6,325
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| rowspan="2" | 75 PE || Full flowcell (2 lane, 800m read) || $1,900 || $2,090 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| One lane (~400m read) || $1,000 || $1,100 || per lane
|-
| rowspan="2" | 150 PE || Full flowcell (2 lane, ~800m read) || $2,650 || $2,915 || per flowcell
|-
| One lane (~400m read) || $1,375 || $1,513 || per lane
|-
| 300 PE || Full flowcell (~200m reads) || $3,500 || $3,850 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100m reads) || $300 || $330 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell (~400m reads)* || $1,800 || $1,980 || per flowcell
|-
| 150 PE: F3 Lane (~100m reads) || $450 || $495 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

{| class="wikitable"
|-
! Length !! Reads !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 300nt || rowspan="2" | 5M || $715 || $786.50 || rowspan="9" | per kit || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || $1,040 || $1,144
|-
| 100nt || rowspan="4" | 25M || $925 || $1,017.50
|-
| 300nt || $1,334 || $1,467.40
|-
| 600nt || $1,570 || $1,727
|-
| '''1000nt''' || $2,590 || $2,849
|-
| 100nt || rowspan="2" | 100M || $1,540 || $1,694
|-
| 300nt || $2,125 || $2,337.50
|-
| 600nt || 50M || $2,125 || $2,337.50
|}

==== MiSeq i100 Walkup ====
Training and lab storage on BMC servers required. Run must be scheduled in the [https://mit.ilabsolutions.com/schedules/555535#/schedule/ iLabs calendar.]

{| class="wikitable"
|-
! Length !! Reads !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! RunTime !! Notes
|-
| Machine usage || — || $120 || $132 || per run || — || training and lab storage on BMC servers required.
|-
| 300nt || rowspan="2" | 5M || $381 || $419.10 || rowspan="9" | per kit || 8h || rowspan="9" |
|-
| 600nt || $665 || $731.50 || 16h
|-
| 100nt || rowspan="4" | 25M || $565 || $621.50 || 5h
|-
| 300nt || $920 || $1,012 || 8h
|-
| 600nt || $1,125 || $1,238 || 16h
|-
| 1000nt || $1,921 || $2,113.10 || 24h
|-
| 100nt || rowspan="2" | 100M || $1,130 || $1,243 || 5h
|-
| 300nt || $1,638.50 || $1,802.35 || 8h
|-
| 600nt || 50M || $1,638.50 || $1,802.35 || 16h
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! Length !! Reads !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 70nt || 15M || $1,320 || $1,452 || rowspan="6" | per kit || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt || 25M || $1,430 || $1,573
|-
| 300nt || 15M || $1,595 || $1,754.50
|-
| 500nt || 15M || $1,760 || $1,936
|-
| 600nt || 25M || $2,200 || $2,420
|-
| 500nt || 1M  '''NANO''' || $770 || $847
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only -only one QC is included per sequencing lane. || $35 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate(short read) || $400 || $440 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200 || $1,320 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| SMRT Cell || $2,900 || $3,190 || per SMRT Cell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! colspan="3" | ILLUMINA Sample Preparation !! colspan="3" style="color:blue;" |  
|-
| colspan="3" style="font-weight:bold; text-align:center;" | '''STANDARD THROUGHPUT DNA'''   Prices for individual samples. Repreps will typically be attempted on failed samples without charge. || colspan="3" style="font-weight:bold; text-align:center; color:blue;" | '''HIGH THROUGHPUT DNA'''   Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| rowspan="3" | LIGATION BASED NEB UltraII || STD || per sample || $105 || $115 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| style="color:blue;" | HTL || style="color:blue;" | 24 || style="color:blue;" | $900 || style="color:blue;" | $990 || rowspan="2" style="color:blue;" | Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| style="color:blue;" | HTL || style="color:blue;" | 96 || style="color:blue;" | $1,800 || style="color:blue;" | $1,980
|-
| Tagmentation   NEXTERA-XT || rowspan="2" | STD || rowspan="2" | per Sample || $105 || $115 || rowspan="2" | Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation   NEXTERA-FLEX || $157 || $173
|-
| rowspan="2" | NEXTERA-XT OR FLEX || style="color:blue;" | HTL || style="color:blue;" | ''24'' || style="color:blue;" | $900 || style="color:blue;" | $990 || rowspan="2" style="color:blue;" | Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| style="color:blue;" | HTL || style="color:blue;" | ''96'' || style="color:blue;" | $1,800 || style="color:blue;" | $1,980
|-
| rowspan="2" | 16S Amplicon || style="color:blue;" | HTL || style="color:blue;" | 48 || style="color:blue;" | $1,900 || style="color:blue;" | $2,090 || rowspan="2" style="color:blue;" | Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| style="color:blue;" | HTL || style="color:blue;" | add'l 48 || style="color:blue;" | $650 || style="color:blue;" | $715
|-
| rowspan="2" | Other Amplicon || style="color:blue;" | HTL || style="color:blue;" | 48 || style="color:blue;" | $1,600 || style="color:blue;" | $1,760 || rowspan="2" style="color:blue;" | Includes normalization, enrichment, pooling and quality control (SYBR and FA).   Samples *must* be submitted amplified with appropriate linkers.
|-
| style="color:blue;" | HTL || style="color:blue;" | add'l 48 || style="color:blue;" | $630 || style="color:blue;" | $693
|-
| colspan="6" style="font-weight:bold; text-align:center;" | '''ADDITIONAL FEES'''   Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $60 || $66 ||
|-
| Sample Cleanup || || per sample || $5 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10 || $11 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! colspan="3" | ILLUMINA Sample Preparation !! colspan="3" style="color:blue;" |  
|-
| colspan="3" style="font-weight:bold; text-align:center;" | '''STANDARD RNA'''   Prices for individual samples. Repreps will typically be attempted on failed samples without charge. || colspan="3" style="font-weight:bold; text-align:center; color:blue;" | '''HIGH THROUGHPUT RNA'''   Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| rowspan="3" | polyA based isolation   NEB UltraII RNAseq || '''STD''' || per sample || $157 || $173 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| style="color:blue;" | '''HTL''' (NEB only) || style="color:blue;" | ''24'' || style="color:blue;" | $1,500 || style="color:blue;" | $1,650 || rowspan="2" style="color:blue;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| style="color:blue;" | '''HTL''' (NEB only) || style="color:blue;" | ''96'' || style="color:blue;" | $3,000 || style="color:blue;" | $3,300
|-
| rowspan="2" | High Throughput 3' Digital Gene Expression (HT3DGE) || style="color:blue;" | '''HTL''' || style="color:blue;" | ''24'' || style="color:blue;" | $1,050 || style="color:blue;" | $1,155 || rowspan="2" style="color:blue;" | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| style="color:blue;" | '''HTL''' || style="color:blue;" | ''96'' || style="color:blue;" | $2,100 || style="color:blue;" | $2,310
|-
| rowspan="3" | Total RNA sequencing   NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| style="color:blue;" | '''HTL''' (NEB only, covaris RNA fragmentation) || style="color:blue;" | ''24'' || style="color:blue;" | $2,500 || style="color:blue;" | $2,750 || rowspan="2" style="color:blue;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| style="color:blue;" | '''HTL''' (NEB only, covaris RNA fragmentation) || style="color:blue;" | ''96'' || style="color:blue;" | $5,000 || style="color:blue;" | $5,500
|-
| rowspan="3" | Low Input polyA+   Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262 || $289 || rowspan="3" | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| style="color:blue;" | '''HTL''' v2 || style="color:blue;" | ''24'' || style="color:blue;" | $2,000 || style="color:blue;" | $2,200
|-
| style="color:blue;" | '''HTL''' v2 || style="color:blue;" | ''96'' || style="color:blue;" | $3,200 || style="color:blue;" | $3,520
|-
| rowspan="3" | Low Input Mammalian Total   Clontech ZapR Kit || STD || per sample || $210 || $231 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| style="color:blue;" | '''HTL''' (covaris RNA fragmentation) || style="color:blue;" | ''24'' || style="color:blue;" | $3,000 || style="color:blue;" | $3,300 || rowspan="2" style="color:blue;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| style="color:blue;" | '''HTL''' (covaris RNA fragmentation) || style="color:blue;" | ''96'' || style="color:blue;" | $5,000 || style="color:blue;" | $5,500
|-
| Small RNA || STD || per sample || $289 || $318 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" style="font-weight:bold; text-align:center;" | '''ADDITIONAL FEES'''   Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $60 || $66 ||
|-
| Sample Cleanup || || per sample || $5 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10 || $11 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit
|-
| Standard Ligation || LSK || $160 || $176 || per sample/pool
|-
| Native Barcoding   (ligation indexing) || - || $50 || $55 || rowspan="5" | per sample
|-
| SMARTseq -> Ligation || Cv4+LSK || $300 || $330
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150 || $165
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262 || $289
|-
| Direct RNA || RNA004 || $160 || $176
|}

{| class="wikitable"
|-
! PacBio Sample Preparation !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit
|-
| rowspan="2" | Basic Library || $315 || $346 || Per Library
|-
| $157 || $173 || Per add'l Sample
|-
| rowspan="2" | Genomic Library || $472 || $520 || Per Library
|-
| $235 || $258 || Per add'l Sample
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| DNA || 96 || $700 || $770 ||
|-
| RNA || 96 || $800 || $880 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300 || $330 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000 || $1,100 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

Please email biomicro@mit.edu two weeks prior to desired submission date so we will have reagents ready for you.

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| rowspan="2" | 5' RNA   3' RNA || setup || $1,015 || $1,116.50 || charge per submission
|-
| per sample || $1,750 || $1,925 || Includes QC of cDNA and creation of Short Read Library
|-
| rowspan="2" | 5' RNA   3' RNA - on chip multiplexing || setup || $1,150 || $1,265 || charge per submission
|-
| per 4 samples || $2,305 || $2,535 || Includes QC of cDNA and creation of Short Read Library
|-
| rowspan="2" | Fixed RNA (Flex v2)   probe based. human/mouse only || setup || $2,700 || $2,970 || charge per submission - up to 48 samples
|-
| per sample (1-plex) || $270 || $297 || Includes QC of cDNA and creation of Short Read Library
|-
| rowspan="2" | Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc // per amplicon) || setup || $470 || $517 || additional prep beyond DGE
|-
| per sample || $45 || $50 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| rowspan="2" | ATACseq || setup || $880 || $968 || charge per submission
|-
| per sample || $1,675 || $1,843 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| rowspan="2" | MULTI || setup || $1,800 || $1,980 || charge per submission
|-
| per sample || $3,100 || $3,410 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || || $200 || $220 || <24h notice
|}

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| HD: polyA or FFPE (hs/mm only) || 1 slide / 2 tiles || $7,850 || $8,596 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $60 || $66 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $40 || $44 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Slide Only || rowspan="5" | per slide || $150 || $165 || slide: 1, 12, or 48 well
|-
| Optimization Only || $800 || $880 || used when the run fails
|-
| [https://www.elementbiosciences.com/products/aviti24/cytoprofiling Teton Defined Panel] || $7,200 || $7,920 || scroll to predefined panels
|-
| Atlas Short || $5,500 || $6,050 || for CRISPR screens / short sequencing
|-
| [https://www.elementbiosciences.com/products/aviti24/cytoprofiling Antibody Add-on] || $360 || $396 || scroll to flexible protein customization
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15 || $16.50 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80 || $88 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30 || $33 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $160 || $176 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50 || $55 ||
|-
| qPCR only (Short read quantification test) || per sample || $25 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400 || $440 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $40 || $44 ||
|-
| FFPE Curl || per curl || $60 || $66 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| rowspan="6" | DNAscript Syntax || Setup (per run) || $1,000 || $1,100 || Per run cost
|-
| 2000 nucleotide synthesis || $500 || $550 || Up to 96 samples
|-
| 8000 nucleotide synthesis || $1,500 || $1,650 || Up to 96 samples
|-
| add Biotin (max48) || $115 || $126 || max 48 per run and 2 per oligo. Couple to T only.
|-
| add Fluor || $300 || $330 ||
|-
| add quench || $100 || $110 ||
|}

== [[BioMicroCenter:RTPCR|REAL TIME PCR]] ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| Roche LightCycler 480 || $15 || per plate || [https://mit.ilabsolutions.com/schedules/261680#/schedule/ CALENDAR] *available for CORE only || Contact [[BioMicroCenter:People|Christopher Hallee]] for training
|-
| SYBR Green (KAPA/Roche) || $45 || per ml || || Available for MIT only
|-
| 96-well PLATES || $10 || per plate || || Available for MIT only
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $20 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $75 || per gel (5 lanes) || TBA ||
|-
| Training || $25 || per session || TBA || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $18 || per hour || [https://mit.ilabsolutions.com/schedules/262715#/schedule/ CALENDAR] || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="5" | '''ROBOTICS'''
|-
| Tecan EVO 150 || $75 || per 1h block - based on scheduled time. || [https://mit.ilabsolutions.com/schedules/262347#/schedule/ CALENDAR] ||
|-
| non-filter tips || $8 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 || per box || ||
|-
| Training || $65 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]] ||
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90 || $125 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100 || $110 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100 || $300 ||
|-
| Tape Archive / Recovery || per tape || $60 || $70 ||
|-
| Informatics Training Classes || per session || $50 || $100 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-27T17:10:38Z

Hilo1: /* MiSeq i100 Assisted */

'''PRICING UPDATE 7/1/2025'''

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850 || $2,000 || rowspan="2" | Per Lane || rowspan="5" | Through collaboration with nearby academic shared resources
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600 || $2,860
|-
| NovaSeqX 10B 300nt^^ || $12,160 || $13,376 || rowspan="3" | Per Flowcell
|-
| NovaSeqX 25B 300nt^^ || $16,200 || $17,820
|-
| NovaSeqX 25B 100nt^^ || $5,750 || $6,325
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| rowspan="2" | 75 PE || Full flowcell (2 lane, 800m read) || $1,900 || $2,090 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| One lane (~400m read) || $1,000 || $1,100 || per lane
|-
| rowspan="2" | 150 PE || Full flowcell (2 lane, ~800m read) || $2,650 || $2,915 || per flowcell
|-
| One lane (~400m read) || $1,375 || $1,513 || per lane
|-
| 300 PE || Full flowcell (~200m reads) || $3,500 || $3,850 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100m reads) || $300 || $330 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell (~400m reads)* || $1,800 || $1,980 || per flowcell
|-
| 150 PE: F3 Lane (~100m reads) || $450 || $495 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

{| class="wikitable"
|-
! Length !! Reads !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 300nt || rowspan="2" | 5M || $715 || $786.50 || rowspan="9" | per kit || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || $1,040 || $1,144
|-
| 100nt || rowspan="4" | 25M || $925 || $1,017.50
|-
| 300nt || $1,334 || $1,467.40
|-
| 600nt || $1,570 || $1,727
|-
| '''1000nt''' || $2,590 || $2,849
|-
| 100nt || rowspan="2" | 100M || $1,540 || $1,694
|-
| 300nt || $2,125 || $2,337.50
|-
| 600nt || 50M || $2,125 || $2,337.50
|}

==== MiSeq i100 Walkup ====
Training and lab storage on BMC servers required. Run must be scheduled in the [https://mit.ilabsolutions.com/schedules/555535#/schedule/ iLabs calendar.]

{| class="wikitable"
|-
! Length !! Reads !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! RunTime !! Notes
|-
| Machine usage || — || $120 || $132 || per run || — || training and lab storage on BMC servers required.
|-
| 300nt || rowspan="2" | 5M || $381 || $419.10 || rowspan="9" | per kit || 8h || rowspan="9" |
|-
| 600nt || $665 || $731.50 || 16h
|-
| 100nt || rowspan="4" | 25M || $565 || $621.50 || 5h
|-
| 300nt || $920 || $1,012 || 8h
|-
| 600nt || $1,125 || $1,238 || 16h
|-
| 1000nt || $1,921 || $2,113.10 || 24h
|-
| 100nt || rowspan="2" | 100M || $1,130 || $1,243 || 5h
|-
| 300nt || $1,638.50 || $1,802.35 || 8h
|-
| 600nt || 50M || $1,638.50 || $1,802.35 || 16h
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! Length !! Reads !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 70nt || 15M || $1,320 || $1,452 || rowspan="6" | per lane || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt || 25M || $1,430 || $1,573
|-
| 300nt || 15M || $1,595 || $1,754.50
|-
| 500nt || 15M || $1,760 || $1,936
|-
| 600nt || 25M || $2,200 || $2,420
|-
| 500nt || 1M  '''NANO''' || $770 || $847
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only -only one QC is included per sequencing lane. || $35 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate(short read) || $400 || $440 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200 || $1,320 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| SMRT Cell || $2,900 || $3,190 || per SMRT Cell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! colspan="3" | ILLUMINA Sample Preparation !! colspan="3" style="color:blue;" |  
|-
| colspan="3" style="font-weight:bold; text-align:center;" | '''STANDARD THROUGHPUT DNA'''   Prices for individual samples. Repreps will typically be attempted on failed samples without charge. || colspan="3" style="font-weight:bold; text-align:center; color:blue;" | '''HIGH THROUGHPUT DNA'''   Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| rowspan="3" | LIGATION BASED NEB UltraII || STD || per sample || $105 || $115 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| style="color:blue;" | HTL || style="color:blue;" | 24 || style="color:blue;" | $900 || style="color:blue;" | $990 || rowspan="2" style="color:blue;" | Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| style="color:blue;" | HTL || style="color:blue;" | 96 || style="color:blue;" | $1,800 || style="color:blue;" | $1,980
|-
| Tagmentation   NEXTERA-XT || rowspan="2" | STD || rowspan="2" | per Sample || $105 || $115 || rowspan="2" | Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation   NEXTERA-FLEX || $157 || $173
|-
| rowspan="2" | NEXTERA-XT OR FLEX || style="color:blue;" | HTL || style="color:blue;" | ''24'' || style="color:blue;" | $900 || style="color:blue;" | $990 || rowspan="2" style="color:blue;" | Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| style="color:blue;" | HTL || style="color:blue;" | ''96'' || style="color:blue;" | $1,800 || style="color:blue;" | $1,980
|-
| rowspan="2" | 16S Amplicon || style="color:blue;" | HTL || style="color:blue;" | 48 || style="color:blue;" | $1,900 || style="color:blue;" | $2,090 || rowspan="2" style="color:blue;" | Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| style="color:blue;" | HTL || style="color:blue;" | add'l 48 || style="color:blue;" | $650 || style="color:blue;" | $715
|-
| rowspan="2" | Other Amplicon || style="color:blue;" | HTL || style="color:blue;" | 48 || style="color:blue;" | $1,600 || style="color:blue;" | $1,760 || rowspan="2" style="color:blue;" | Includes normalization, enrichment, pooling and quality control (SYBR and FA).   Samples *must* be submitted amplified with appropriate linkers.
|-
| style="color:blue;" | HTL || style="color:blue;" | add'l 48 || style="color:blue;" | $630 || style="color:blue;" | $693
|-
| colspan="6" style="font-weight:bold; text-align:center;" | '''ADDITIONAL FEES'''   Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $60 || $66 ||
|-
| Sample Cleanup || || per sample || $5 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10 || $11 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! colspan="3" | ILLUMINA Sample Preparation !! colspan="3" style="color:blue;" |  
|-
| colspan="3" style="font-weight:bold; text-align:center;" | '''STANDARD RNA'''   Prices for individual samples. Repreps will typically be attempted on failed samples without charge. || colspan="3" style="font-weight:bold; text-align:center; color:blue;" | '''HIGH THROUGHPUT RNA'''   Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| rowspan="3" | polyA based isolation   NEB UltraII RNAseq || '''STD''' || per sample || $157 || $173 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| style="color:blue;" | '''HTL''' (NEB only) || style="color:blue;" | ''24'' || style="color:blue;" | $1,500 || style="color:blue;" | $1,650 || rowspan="2" style="color:blue;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| style="color:blue;" | '''HTL''' (NEB only) || style="color:blue;" | ''96'' || style="color:blue;" | $3,000 || style="color:blue;" | $3,300
|-
| rowspan="2" | High Throughput 3' Digital Gene Expression (HT3DGE) || style="color:blue;" | '''HTL''' || style="color:blue;" | ''24'' || style="color:blue;" | $1,050 || style="color:blue;" | $1,155 || rowspan="2" style="color:blue;" | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| style="color:blue;" | '''HTL''' || style="color:blue;" | ''96'' || style="color:blue;" | $2,100 || style="color:blue;" | $2,310
|-
| rowspan="3" | Total RNA sequencing   NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| style="color:blue;" | '''HTL''' (NEB only, covaris RNA fragmentation) || style="color:blue;" | ''24'' || style="color:blue;" | $2,500 || style="color:blue;" | $2,750 || rowspan="2" style="color:blue;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| style="color:blue;" | '''HTL''' (NEB only, covaris RNA fragmentation) || style="color:blue;" | ''96'' || style="color:blue;" | $5,000 || style="color:blue;" | $5,500
|-
| rowspan="3" | Low Input polyA+   Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262 || $289 || rowspan="3" | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| style="color:blue;" | '''HTL''' v2 || style="color:blue;" | ''24'' || style="color:blue;" | $2,000 || style="color:blue;" | $2,200
|-
| style="color:blue;" | '''HTL''' v2 || style="color:blue;" | ''96'' || style="color:blue;" | $3,200 || style="color:blue;" | $3,520
|-
| rowspan="3" | Low Input Mammalian Total   Clontech ZapR Kit || STD || per sample || $210 || $231 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| style="color:blue;" | '''HTL''' (covaris RNA fragmentation) || style="color:blue;" | ''24'' || style="color:blue;" | $3,000 || style="color:blue;" | $3,300 || rowspan="2" style="color:blue;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| style="color:blue;" | '''HTL''' (covaris RNA fragmentation) || style="color:blue;" | ''96'' || style="color:blue;" | $5,000 || style="color:blue;" | $5,500
|-
| Small RNA || STD || per sample || $289 || $318 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" style="font-weight:bold; text-align:center;" | '''ADDITIONAL FEES'''   Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $60 || $66 ||
|-
| Sample Cleanup || || per sample || $5 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10 || $11 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit
|-
| Standard Ligation || LSK || $160 || $176 || per sample/pool
|-
| Native Barcoding   (ligation indexing) || - || $50 || $55 || rowspan="5" | per sample
|-
| SMARTseq -> Ligation || Cv4+LSK || $300 || $330
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150 || $165
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262 || $289
|-
| Direct RNA || RNA004 || $160 || $176
|}

{| class="wikitable"
|-
! PacBio Sample Preparation !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit
|-
| rowspan="2" | Basic Library || $315 || $346 || Per Library
|-
| $157 || $173 || Per add'l Sample
|-
| rowspan="2" | Genomic Library || $472 || $520 || Per Library
|-
| $235 || $258 || Per add'l Sample
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| DNA || 96 || $700 || $770 ||
|-
| RNA || 96 || $800 || $880 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300 || $330 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000 || $1,100 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

Please email biomicro@mit.edu two weeks prior to desired submission date so we will have reagents ready for you.

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| rowspan="2" | 5' RNA   3' RNA || setup || $1,015 || $1,116.50 || charge per submission
|-
| per sample || $1,750 || $1,925 || Includes QC of cDNA and creation of Short Read Library
|-
| rowspan="2" | 5' RNA   3' RNA - on chip multiplexing || setup || $1,150 || $1,265 || charge per submission
|-
| per 4 samples || $2,305 || $2,535 || Includes QC of cDNA and creation of Short Read Library
|-
| rowspan="2" | Fixed RNA (Flex v2)   probe based. human/mouse only || setup || $2,700 || $2,970 || charge per submission - up to 48 samples
|-
| per sample (1-plex) || $270 || $297 || Includes QC of cDNA and creation of Short Read Library
|-
| rowspan="2" | Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc // per amplicon) || setup || $470 || $517 || additional prep beyond DGE
|-
| per sample || $45 || $50 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| rowspan="2" | ATACseq || setup || $880 || $968 || charge per submission
|-
| per sample || $1,675 || $1,843 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| rowspan="2" | MULTI || setup || $1,800 || $1,980 || charge per submission
|-
| per sample || $3,100 || $3,410 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || || $200 || $220 || <24h notice
|}

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| HD: polyA or FFPE (hs/mm only) || 1 slide / 2 tiles || $7,850 || $8,596 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $60 || $66 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $40 || $44 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Slide Only || rowspan="5" | per slide || $150 || $165 || slide: 1, 12, or 48 well
|-
| Optimization Only || $800 || $880 || used when the run fails
|-
| [https://www.elementbiosciences.com/products/aviti24/cytoprofiling Teton Defined Panel] || $7,200 || $7,920 || scroll to predefined panels
|-
| Atlas Short || $5,500 || $6,050 || for CRISPR screens / short sequencing
|-
| [https://www.elementbiosciences.com/products/aviti24/cytoprofiling Antibody Add-on] || $360 || $396 || scroll to flexible protein customization
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15 || $16.50 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80 || $88 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30 || $33 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $160 || $176 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50 || $55 ||
|-
| qPCR only (Short read quantification test) || per sample || $25 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400 || $440 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $40 || $44 ||
|-
| FFPE Curl || per curl || $60 || $66 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| rowspan="6" | DNAscript Syntax || Setup (per run) || $1,000 || $1,100 || Per run cost
|-
| 2000 nucleotide synthesis || $500 || $550 || Up to 96 samples
|-
| 8000 nucleotide synthesis || $1,500 || $1,650 || Up to 96 samples
|-
| add Biotin (max48) || $115 || $126 || max 48 per run and 2 per oligo. Couple to T only.
|-
| add Fluor || $300 || $330 ||
|-
| add quench || $100 || $110 ||
|}

== [[BioMicroCenter:RTPCR|REAL TIME PCR]] ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| Roche LightCycler 480 || $15 || per plate || [https://mit.ilabsolutions.com/schedules/261680#/schedule/ CALENDAR] *available for CORE only || Contact [[BioMicroCenter:People|Christopher Hallee]] for training
|-
| SYBR Green (KAPA/Roche) || $45 || per ml || || Available for MIT only
|-
| 96-well PLATES || $10 || per plate || || Available for MIT only
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $20 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $75 || per gel (5 lanes) || TBA ||
|-
| Training || $25 || per session || TBA || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $18 || per hour || [https://mit.ilabsolutions.com/schedules/262715#/schedule/ CALENDAR] || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="5" | '''ROBOTICS'''
|-
| Tecan EVO 150 || $75 || per 1h block - based on scheduled time. || [https://mit.ilabsolutions.com/schedules/262347#/schedule/ CALENDAR] ||
|-
| non-filter tips || $8 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 || per box || ||
|-
| Training || $65 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]] ||
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90 || $125 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100 || $110 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100 || $300 ||
|-
| Tape Archive / Recovery || per tape || $60 || $70 ||
|-
| Informatics Training Classes || per session || $50 || $100 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

BioMicroCenter:Pricing

2026-04-27T17:08:31Z

Hilo1: /* CHEMAGIC360 */

{{BioMicroCenter}}
'''PRICING UPDATE 7/1/2025'''

== SEQUENCING - MID OUTPUT ==

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! unit !! Notes
|-
| rowspan="2" | 75 PE || Full flowcell (2 lane, 800m read) || $2,375 || $2,660 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| One lane (~400m read) || $1,250 || $1,400 || per lane
|-
| rowspan="2" | 150 PE || Full flowcell (2 lane, ~800m read) || $3,312.50 || $3,710 || per flowcell
|-
| One lane (~400m read) || $1,719 || $1,925 || per lane
|-
| 300 PE || Full flowcell (~200m reads) || $4,375 || $4,900 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! unit !! Notes
|-
| 50 PE: F3 Lane (~100m reads) || $375 || $420 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell (~400m reads)* || $2,250 || $2,520 || per flowcell
|-
| 150 PE: F3 Lane (~100m reads) || $562.50 || $630 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

{| class="wikitable"
|-
! Length !! Reads !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! unit !! Notes
|-
| 300nt || rowspan="2" | 5M || $893.75 || $1,001 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || $1,300 || $1,456
|-
| 100nt || rowspan="4" | 25M || $1,156.25 || $1,295
|-
| 300nt || $1,667.50 || $1,867.60
|-
| 600nt || $1,962.50 || $2,198
|-
| '''1000nt''' || $3,237.50 || $3,626
|-
| 100nt || rowspan="2" | 100M || $1,925 || $2,156
|-
| 300nt || $2,656 || $2,975
|-
| 600nt || 50M || $2,656 || $2,975
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! Length !! Reads !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! unit !! Notes
|-
| 70nt || 15M || $1,650 || $1,848 || rowspan="6" | per lane || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt || 25M || $1,787.50 || $2,002
|-
| 300nt || 15M || $1,993.75 || $2,233
|-
| 500nt || 15M || $2,200 || $2,464
|-
| 600nt || 25M || $2,750 || $3,080
|-
| 500nt || 1M  '''NANO''' || $962.50 || $1,078
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! unit !! Notes
|-
| Quality Control Only -only one QC is included per sequencing lane. || $43.75 || $49 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $18.75 || $21 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $31.25 || $35 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate(short read) || $500 || $560 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! unit !! Notes
|-
| 72 hour Flowcell || $1,500 || $1,680 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! colspan="3" | ILLUMINA Sample Preparation !! colspan="3" style="color:blue;" |  
|-
| colspan="3" style="font-weight:bold; text-align:center;" | '''STANDARD THROUGHPUT DNA'''   Prices for individual samples. Repreps will typically be attempted on failed samples without charge. || colspan="3" style="font-weight:bold; text-align:center; color:blue;" | '''HIGH THROUGHPUT DNA'''   Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! Notes
|-
| rowspan="3" | LIGATION BASED NEB UltraII || STD || per sample || $136.50 || $157 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| style="color:blue;" rowspan="2" | HTL || style="color:blue;" | 24 || style="color:blue;" | $1,125 || style="color:blue;" | $1,260 || rowspan="2" style="color:blue;" | Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| style="color:blue;" | 96 || style="color:blue;" | $2,250 || style="color:blue;" | $2,520
|-
| Tagmentation   NEXTERA-XT || rowspan="2" | STD || rowspan="2" | per Sample || $136.50 || $157 || rowspan="2" | Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation   NEXTERA-FLEX || $204 || $235
|-
| rowspan="2" | NEXTERA-XT OR FLEX || style="color:blue;" rowspan="2" | HTL || style="color:blue;" | ''24'' || style="color:blue;" | $1,125 || style="color:blue;" | $1,260 || rowspan="2" style="color:blue;" | Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| style="color:blue;" | ''96'' || style="color:blue;" | $2,250 || style="color:blue;" | $2,520
|-
| rowspan="2" | 16S Amplicon || style="color:blue;" | HTL || style="color:blue;" | 48 || style="color:blue;" | $2,375 || style="color:blue;" | $2,660 || rowspan="2" style="color:blue;" | Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| style="color:blue;" | HTL || style="color:blue;" | add'l 48 || style="color:blue;" | $812.50 || style="color:blue;" | $910
|-
| rowspan="2" | Other Amplicon || style="color:blue;" | HTL || style="color:blue;" | 48 || style="color:blue;" | $2,000 || style="color:blue;" | $2,240 || rowspan="2" style="color:blue;" | Includes normalization, enrichment, pooling and quality control (SYBR and FA).   Samples *must* be submitted amplified with appropriate linkers.
|-
| style="color:blue;" | HTL || style="color:blue;" | add'l 48 || style="color:blue;" | $787.50 || style="color:blue;" | $945
|-
| colspan="6" style="font-weight:bold; text-align:center;" | '''ADDITIONAL FEES'''   Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $75 || $84
|-
| Sample Cleanup || || per sample || $6.25 || $7 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! colspan="3" | ILLUMINA Sample Preparation !! colspan="3" style="color:blue;" |  
|-
| colspan="3" style="font-weight:bold; text-align:center;" | '''STANDARD RNA'''   Prices for individual samples. Repreps will typically be attempted on failed samples without charge. || colspan="3" style="font-weight:bold; text-align:center; color:blue;" | '''HIGH THROUGHPUT RNA'''   Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! Notes
|-
| rowspan="3" | polyA based isolation   NEB UltraII RNAseq || '''STD''' || per sample || $196.25 || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| style="color:blue;" | rowspan="2" | '''HTL''' (NEB only) || style="color:blue;" | ''24'' || style="color:blue;" | $1,875 || style="color:blue;" | $2,100 || rowspan="2" style="color:blue;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| style="color:blue;" | ''96'' || style="color:blue;" | $3,750 || style="color:blue;" | $4,200
|-
| rowspan="2" | High Throughput 3' Digital Gene Expression (HT3DGE) || style="color:blue;" | rowspan="2" | '''HTL''' || style="color:blue;" | ''24'' || style="color:blue;" | $1,312.50 || style="color:blue;" | $1,470 || rowspan="2" style="color:blue;" | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| style="color:blue;" | ''96'' || style="color:blue;" | $2,625 || style="color:blue;" | $2,940
|-
| rowspan="3" | Total RNA sequencing   NEB RNAseq +Ribodepletion || '''STD''' || per sample || $293.75 || $329 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| style="color:blue;" | rowspan="2" | '''HTL''' (NEB only, covaris RNA fragmentation) || style="color:blue;" | ''24'' || style="color:blue;" | $3,125 || style="color:blue;" | $3,500 || rowspan="2" style="color:blue;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| style="color:blue;" | ''96'' || style="color:blue;" | $6,250 || style="color:blue;" | $7,000
|-
| rowspan="3" | Low Input polyA+   Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $340.60 || $394 || rowspan="3" | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| style="color:blue;" | rowspan="2" | '''HTL''' v2 || style="color:blue;" | ''24'' || style="color:blue;" | $2,500 || style="color:blue;" | $2,800
|-
| style="color:blue;" | ''96'' || style="color:blue;" | $4,000 || style="color:blue;" | $4,480
|-
| rowspan="3" | Low Input Mammalian Total   Clontech ZapR Kit || STD || per sample || $273 || $315 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| style="color:blue;" | rowspan="2" | '''HTL''' (covaris RNA fragmentation) || style="color:blue;" | ''24'' || style="color:blue;" | $3,750 || style="color:blue;" | $4,200 || rowspan="2" style="color:blue;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| style="color:blue;" | ''96'' || style="color:blue;" | $6,250 || style="color:blue;" | $7,000
|-
| Small RNA || STD || per sample || $375.70 || $433 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" style="font-weight:bold; text-align:center;" | '''ADDITIONAL FEES'''   Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $75 || $84
|-
| Sample Cleanup || || per sample || $6.25 || $7 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! unit
|-
| Standard Ligation || LSK || $200 || $224 || per sample/pool
|-
| Native Barcoding   (ligation indexing) || - || $65 || $70 || rowspan="5" | per sample
|-
| SMARTseq -> Ligation || Cv4+LSK || $375 || $420
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $188 || $225
|-
| Long Insert Prep || RAD (modified, target >100kb) || $340.60 || $393
|-
| Direct RNA || RNA004 || $200 || $240
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit
|-
| DNA || 96 || $875 || $980
|-
| RNA || 96 || $1,000 || $1,120
|-
| RNA/DNA Isolation - Assisted || 12 samples || $375 || $420
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,250 || $1,400
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

Please email biomicro@mit.edu two weeks prior to desired submission date so we will have reagents ready for you.

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| rowspan="2" | 5' RNA   3' RNA || setup || $1,268.75 || $1,421 || charge per submission
|-
| per sample || $2,188 || $2,450 || Includes QC of cDNA and creation of Short Read Library
|-
| rowspan="2" | 5' RNA   3' RNA - on chip multiplexing || setup || $1,437.50 || $1,610 || charge per submission
|-
| per 4 samples || $2,881 || $3,227 || Includes QC of cDNA and creation of Short Read Library
|-
| rowspan="2" | Fixed RNA (Flex v2)   probe based. human/mouse only || setup || $3,375 || $3,780 || charge per submission - up to 48 samples
|-
| per sample (1-plex) || $337.50 || $378 || Includes QC of cDNA and creation of Short Read Library
|-
| rowspan="2" | Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc // per amplicon) || setup || $587.50 || $658 || additional prep beyond DGE
|-
| per sample || $56 || $63 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| rowspan="2" | ATACseq || setup || $1,100 || $1,232 || charge per submission
|-
| per sample || $2,094 || $2,345 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| rowspan="2" | MULTI || setup || $2,250 || $2,520 || charge per submission
|-
| per sample || $3,875 || $4,340 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || || $250 || $280 || <24h notice
|}

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! Notes
|-
| HD: polyA or FFPE (hs/mm only) || 1 slide / 2 tiles || $9,813 || $10,940 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $75 || $84 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $56 || $66 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! Notes
|-
| Slide Only || rowspan="5" | per slide || $188 || $210 || slide: 1, 12, or 48 well
|-
| Optimization Only || $1,000 || $1,120 || used when the run fails
|-
| [https://www.elementbiosciences.com/products/aviti24/cytoprofiling Teton Defined Panel] || $9,000 || $10,080 || scroll to predefined panels
|-
| Atlas Short || $6,875 || $7,700 || for CRISPR screens / short sequencing
|-
| [https://www.elementbiosciences.com/products/aviti24/cytoprofiling Antibody Add-on] || $450 || $504 || scroll to flexible protein customization
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $18.75 || $21
|-
| Fragment Analyzer: Preloaded row || per row (12) || $100 || $112 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $37.50 || $42
|-
| FemtoPulse: Preloaded Row || per row (12) || $200 || $224 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $62.50 || $70
|-
| qPCR only (Short read quantification test) || per sample || $31.25 || $35 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $500 || $560 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $56 || $66 ||
|-
| FFPE Curl || per curl || $75 || $84 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! Notes
|-
| rowspan="6" | DNAscript Syntax || Setup (per run) || $1,250 || $1,400 || Per run cost
|-
| 2000 nucleotide synthesis || $625 || $700 || Up to 96 samples
|-
| 8000 nucleotide synthesis || $1,875 || $2,100 || Up to 96 samples
|-
| add Biotin (max48) || $144 || $161 || max 48 per run and 2 per oligo. Couple to T only.
|-
| add Fluor || $375 || $420 ||
|-
| add quench || $125 || $140 ||
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External For-Profit]] !! Notes
|-
| Informatics Support || per hour || $180 || $205 || Very limited support for non-MIT users.
|}

MIT:Pricing

2026-04-15T19:28:00Z

Hilo1: /* OLIGO SYNTHESIS */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00
|-
| NovaSeq S4 50nt Lane**|| $3,780.00 || $4,158.00
|-
| NovaSeq S4 300nt Lane**|| $5,400.00 || $5,940.00
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00
|-
| NovaSeqX 10B 300nt^^ || $12,160.00 || $13,376.00
|-
| NovaSeqX 25B 300nt^^ || $16,200.00 || $17,820.00
|-
| NovaSeqX 25B 100nt^^ || $5,750.00 || $6,325.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

{| class="wikitable"
|-
!i100 kit!! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
|5M 300nt || $715.00 || $786.50
|-
|5M 600nt || $1,040.00 || $1,144.00
|-
|25M 100nt || $925.00 || $1,017.50
|-
|25M 300nt|| $1,334.00 || $1,467.40
|-
|25M 600nt || $1,570.00 || $1,727.00
|-
|25M 1000nt|| $2,590.00 || $2,849.00
|-
|100M 100nt || $1,540.00 || $1,694.00
|-
|100M 300nt || $2,125.00 || $2,337.50
|-
|50M 600nt || $2,125.00 || $2,337.50
|}

==== MiSeq i100 Walkup ====
Training and lab storage on BMC servers required. Run must be scheduled in the [https://mit.ilabsolutions.com/schedules/555535#/schedule/ iLabs calendar.]
{| class="wikitable"
|-
!i100 Kit!! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime
|-
| Machine usage || $120.00 || $132.00 || Xh
|-
|5M 300nt|| $381.00 || $419.10 || 8h
|-
|5M 600nt || $665.00 || $731.50 || 16h
|-
|25M 100nt || $565.00 || $621.50 || 5h
|-
|25M 300nt || $920.00 || $1,012.00 || 8h
|-
|25M 600nt || $1,125.00 || $1,238.00 || 16h
|-
|25M 1000nt || $1,921.00 || $2,113.10 || 24h
|-
|100M 100nt || $1,130.00 || $1,243.00 || 5h
|-
|100M 300nt || $1,638.50 || $1,802.35 || 8h
|-
|50M 600nt || $1,638.50 || $1,802.35 || 16h
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| 70nt V2 || $1,320.00 || $1,452.00
|-
| 150nt V3 || $1,430.00 || $1,573.00
|-
| 300nt V2 || $1,595.00 || $1,754.50
|-
| 500nt V2 || $1,760.00 || $1,936.00
|-
| 600nt V3 || $2,200.00 || $2,420.00
|-
| 500nt '''NANO''' Run || $770.00 || $847.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples submitted not in a plated format will be subject to these additional charges.
|-
| High Throughput Setup || STD || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || STD || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || STD || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" |'''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || STD || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || STD || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || STD v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || HTL (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || HTL (NEB only) || 96 || $3,000.00 || $3,300.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || HTL || 96 || $2,100.00 || $2,310.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00
|-
| || || 96 || $3,200.00 || $3,520.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || STD || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || STD || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || STD || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===
Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Standard Ligation || LSK || $160.00 || $176.00
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00
|-
| Direct RNA || RNA004 || $160.00 || $176.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| DNA || 96 || $700.00 || $770.00
|-
| RNA || 96 || $800.00 || $880.00
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===
Please email biomicro@mit.edu two weeks prior to desired submission date so we will have reagents ready for you.

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $200.00 || $220.00
|}

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Slide Only || per slide || $150.00 || $165.00
|-
| Optimization Only || per slide || $800.00 || $880.00
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00
|-
| Antibody Add-on || per slide || $360.00 || $396.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00
|-
| FFPE Curl || per curl || $60.00 || $66.00
|}

=== OLIGO SYNTHESIS ===
The Syntax 600 from DNAScript is an oligo synthesizer for oligos at biologically relevant concentrations.
{| class="wikitable"
|-
! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| add Fluor || $300.00 || $330.00 ||
|-
| add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

Email biomicro@mit.edu for training

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! iLabs
|-
| Roche LightCycler 480 || $15.00 || per plate ||[https://mit.ilabsolutions.com/schedules/261680#/schedule/ ilab qPCR calendar]
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || [https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|-
| 96-well PLATES || $10.00 || per plate ||[https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $18.00 / $20.00 || per hour || [https://mit.ilabsolutions.com/schedules/262715#/schedule/ ilabs plate reader calendar] || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''ROBOTICS'''
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || [https://mit.ilabsolutions.com/service_center/3381/?tab=services services request]
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-15T18:55:37Z

Hilo1: /* OLIGO SYNTHESIS */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00
|-
| NovaSeq S4 50nt Lane**|| $3,780.00 || $4,158.00
|-
| NovaSeq S4 300nt Lane**|| $5,400.00 || $5,940.00
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00
|-
| NovaSeqX 10B 300nt^^ || $12,160.00 || $13,376.00
|-
| NovaSeqX 25B 300nt^^ || $16,200.00 || $17,820.00
|-
| NovaSeqX 25B 100nt^^ || $5,750.00 || $6,325.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

{| class="wikitable"
|-
!i100 kit!! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
|5M 300nt || $715.00 || $786.50
|-
|5M 600nt || $1,040.00 || $1,144.00
|-
|25M 100nt || $925.00 || $1,017.50
|-
|25M 300nt|| $1,334.00 || $1,467.40
|-
|25M 600nt || $1,570.00 || $1,727.00
|-
|25M 1000nt|| $2,590.00 || $2,849.00
|-
|100M 100nt || $1,540.00 || $1,694.00
|-
|100M 300nt || $2,125.00 || $2,337.50
|-
|50M 600nt || $2,125.00 || $2,337.50
|}

==== MiSeq i100 Walkup ====
Training and lab storage on BMC servers required. Run must be scheduled in the [https://mit.ilabsolutions.com/schedules/555535#/schedule/ iLabs calendar.]
{| class="wikitable"
|-
!i100 Kit!! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime
|-
| Machine usage || $120.00 || $132.00 || Xh
|-
|5M 300nt|| $381.00 || $419.10 || 8h
|-
|5M 600nt || $665.00 || $731.50 || 16h
|-
|25M 100nt || $565.00 || $621.50 || 5h
|-
|25M 300nt || $920.00 || $1,012.00 || 8h
|-
|25M 600nt || $1,125.00 || $1,238.00 || 16h
|-
|25M 1000nt || $1,921.00 || $2,113.10 || 24h
|-
|100M 100nt || $1,130.00 || $1,243.00 || 5h
|-
|100M 300nt || $1,638.50 || $1,802.35 || 8h
|-
|50M 600nt || $1,638.50 || $1,802.35 || 16h
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| 70nt V2 || $1,320.00 || $1,452.00
|-
| 150nt V3 || $1,430.00 || $1,573.00
|-
| 300nt V2 || $1,595.00 || $1,754.50
|-
| 500nt V2 || $1,760.00 || $1,936.00
|-
| 600nt V3 || $2,200.00 || $2,420.00
|-
| 500nt '''NANO''' Run || $770.00 || $847.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples submitted not in a plated format will be subject to these additional charges.
|-
| High Throughput Setup || STD || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || STD || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || STD || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" |'''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || STD || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || STD || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || STD v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || HTL (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || HTL (NEB only) || 96 || $3,000.00 || $3,300.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || HTL || 96 || $2,100.00 || $2,310.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00
|-
| || || 96 || $3,200.00 || $3,520.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || STD || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || STD || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || STD || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===
Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Standard Ligation || LSK || $160.00 || $176.00
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00
|-
| Direct RNA || RNA004 || $160.00 || $176.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| DNA || 96 || $700.00 || $770.00
|-
| RNA || 96 || $800.00 || $880.00
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===
Please email biomicro@mit.edu two weeks prior to desired submission date so we will have reagents ready for you.

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $200.00 || $220.00
|}

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Slide Only || per slide || $150.00 || $165.00
|-
| Optimization Only || per slide || $800.00 || $880.00
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00
|-
| Antibody Add-on || per slide || $360.00 || $396.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00
|-
| FFPE Curl || per curl || $60.00 || $66.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | Dr.Oligo
|-
| Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| colspan="6" | DNAscript Syntax
|-
| Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $300.00 || $330.00 ||
|-
| || add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

Email biomicro@mit.edu for training

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! iLabs
|-
| Roche LightCycler 480 || $15.00 || per plate ||[https://mit.ilabsolutions.com/schedules/261680#/schedule/ ilab qPCR calendar]
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || [https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|-
| 96-well PLATES || $10.00 || per plate ||[https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $18.00 / $20.00 || per hour || [https://mit.ilabsolutions.com/schedules/262715#/schedule/ ilabs plate reader calendar] || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''ROBOTICS'''
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || [https://mit.ilabsolutions.com/service_center/3381/?tab=services services request]
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-15T18:52:51Z

Hilo1: /* 10X CHROMIUM */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00
|-
| NovaSeq S4 50nt Lane**|| $3,780.00 || $4,158.00
|-
| NovaSeq S4 300nt Lane**|| $5,400.00 || $5,940.00
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00
|-
| NovaSeqX 10B 300nt^^ || $12,160.00 || $13,376.00
|-
| NovaSeqX 25B 300nt^^ || $16,200.00 || $17,820.00
|-
| NovaSeqX 25B 100nt^^ || $5,750.00 || $6,325.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

{| class="wikitable"
|-
!i100 kit!! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
|5M 300nt || $715.00 || $786.50
|-
|5M 600nt || $1,040.00 || $1,144.00
|-
|25M 100nt || $925.00 || $1,017.50
|-
|25M 300nt|| $1,334.00 || $1,467.40
|-
|25M 600nt || $1,570.00 || $1,727.00
|-
|25M 1000nt|| $2,590.00 || $2,849.00
|-
|100M 100nt || $1,540.00 || $1,694.00
|-
|100M 300nt || $2,125.00 || $2,337.50
|-
|50M 600nt || $2,125.00 || $2,337.50
|}

==== MiSeq i100 Walkup ====
Training and lab storage on BMC servers required. Run must be scheduled in the [https://mit.ilabsolutions.com/schedules/555535#/schedule/ iLabs calendar.]
{| class="wikitable"
|-
!i100 Kit!! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime
|-
| Machine usage || $120.00 || $132.00 || Xh
|-
|5M 300nt|| $381.00 || $419.10 || 8h
|-
|5M 600nt || $665.00 || $731.50 || 16h
|-
|25M 100nt || $565.00 || $621.50 || 5h
|-
|25M 300nt || $920.00 || $1,012.00 || 8h
|-
|25M 600nt || $1,125.00 || $1,238.00 || 16h
|-
|25M 1000nt || $1,921.00 || $2,113.10 || 24h
|-
|100M 100nt || $1,130.00 || $1,243.00 || 5h
|-
|100M 300nt || $1,638.50 || $1,802.35 || 8h
|-
|50M 600nt || $1,638.50 || $1,802.35 || 16h
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| 70nt V2 || $1,320.00 || $1,452.00
|-
| 150nt V3 || $1,430.00 || $1,573.00
|-
| 300nt V2 || $1,595.00 || $1,754.50
|-
| 500nt V2 || $1,760.00 || $1,936.00
|-
| 600nt V3 || $2,200.00 || $2,420.00
|-
| 500nt '''NANO''' Run || $770.00 || $847.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples submitted not in a plated format will be subject to these additional charges.
|-
| High Throughput Setup || STD || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || STD || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || STD || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" |'''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || STD || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || STD || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || STD v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || HTL (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || HTL (NEB only) || 96 || $3,000.00 || $3,300.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || HTL || 96 || $2,100.00 || $2,310.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00
|-
| || || 96 || $3,200.00 || $3,520.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || STD || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || STD || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || STD || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===
Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Standard Ligation || LSK || $160.00 || $176.00
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00
|-
| Direct RNA || RNA004 || $160.00 || $176.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| DNA || 96 || $700.00 || $770.00
|-
| RNA || 96 || $800.00 || $880.00
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===
Please email biomicro@mit.edu two weeks prior to desired submission date so we will have reagents ready for you.

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $200.00 || $220.00
|}

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Slide Only || per slide || $150.00 || $165.00
|-
| Optimization Only || per slide || $800.00 || $880.00
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00
|-
| Antibody Add-on || per slide || $360.00 || $396.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00
|-
| FFPE Curl || per curl || $60.00 || $66.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $300.00 || $330.00 ||
|-
| || add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

Email biomicro@mit.edu for training

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! iLabs
|-
| Roche LightCycler 480 || $15.00 || per plate ||[https://mit.ilabsolutions.com/schedules/261680#/schedule/ ilab qPCR calendar]
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || [https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|-
| 96-well PLATES || $10.00 || per plate ||[https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $18.00 / $20.00 || per hour || [https://mit.ilabsolutions.com/schedules/262715#/schedule/ ilabs plate reader calendar] || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''ROBOTICS'''
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || [https://mit.ilabsolutions.com/service_center/3381/?tab=services services request]
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-15T18:51:21Z

Hilo1: /* LONG READ LIBRARIES */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00
|-
| NovaSeq S4 50nt Lane**|| $3,780.00 || $4,158.00
|-
| NovaSeq S4 300nt Lane**|| $5,400.00 || $5,940.00
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00
|-
| NovaSeqX 10B 300nt^^ || $12,160.00 || $13,376.00
|-
| NovaSeqX 25B 300nt^^ || $16,200.00 || $17,820.00
|-
| NovaSeqX 25B 100nt^^ || $5,750.00 || $6,325.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

{| class="wikitable"
|-
!i100 kit!! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
|5M 300nt || $715.00 || $786.50
|-
|5M 600nt || $1,040.00 || $1,144.00
|-
|25M 100nt || $925.00 || $1,017.50
|-
|25M 300nt|| $1,334.00 || $1,467.40
|-
|25M 600nt || $1,570.00 || $1,727.00
|-
|25M 1000nt|| $2,590.00 || $2,849.00
|-
|100M 100nt || $1,540.00 || $1,694.00
|-
|100M 300nt || $2,125.00 || $2,337.50
|-
|50M 600nt || $2,125.00 || $2,337.50
|}

==== MiSeq i100 Walkup ====
Training and lab storage on BMC servers required. Run must be scheduled in the [https://mit.ilabsolutions.com/schedules/555535#/schedule/ iLabs calendar.]
{| class="wikitable"
|-
!i100 Kit!! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime
|-
| Machine usage || $120.00 || $132.00 || Xh
|-
|5M 300nt|| $381.00 || $419.10 || 8h
|-
|5M 600nt || $665.00 || $731.50 || 16h
|-
|25M 100nt || $565.00 || $621.50 || 5h
|-
|25M 300nt || $920.00 || $1,012.00 || 8h
|-
|25M 600nt || $1,125.00 || $1,238.00 || 16h
|-
|25M 1000nt || $1,921.00 || $2,113.10 || 24h
|-
|100M 100nt || $1,130.00 || $1,243.00 || 5h
|-
|100M 300nt || $1,638.50 || $1,802.35 || 8h
|-
|50M 600nt || $1,638.50 || $1,802.35 || 16h
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| 70nt V2 || $1,320.00 || $1,452.00
|-
| 150nt V3 || $1,430.00 || $1,573.00
|-
| 300nt V2 || $1,595.00 || $1,754.50
|-
| 500nt V2 || $1,760.00 || $1,936.00
|-
| 600nt V3 || $2,200.00 || $2,420.00
|-
| 500nt '''NANO''' Run || $770.00 || $847.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples submitted not in a plated format will be subject to these additional charges.
|-
| High Throughput Setup || STD || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || STD || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || STD || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" |'''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || STD || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || STD || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || STD v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || HTL (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || HTL (NEB only) || 96 || $3,000.00 || $3,300.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || HTL || 96 || $2,100.00 || $2,310.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00
|-
| || || 96 || $3,200.00 || $3,520.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || STD || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || STD || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || STD || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===
Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Standard Ligation || LSK || $160.00 || $176.00
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00
|-
| Direct RNA || RNA004 || $160.00 || $176.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| DNA || 96 || $700.00 || $770.00
|-
| RNA || 96 || $800.00 || $880.00
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $200.00 || $220.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Slide Only || per slide || $150.00 || $165.00
|-
| Optimization Only || per slide || $800.00 || $880.00
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00
|-
| Antibody Add-on || per slide || $360.00 || $396.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00
|-
| FFPE Curl || per curl || $60.00 || $66.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $300.00 || $330.00 ||
|-
| || add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

Email biomicro@mit.edu for training

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! iLabs
|-
| Roche LightCycler 480 || $15.00 || per plate ||[https://mit.ilabsolutions.com/schedules/261680#/schedule/ ilab qPCR calendar]
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || [https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|-
| 96-well PLATES || $10.00 || per plate ||[https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $18.00 / $20.00 || per hour || [https://mit.ilabsolutions.com/schedules/262715#/schedule/ ilabs plate reader calendar] || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''ROBOTICS'''
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || [https://mit.ilabsolutions.com/service_center/3381/?tab=services services request]
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-15T18:50:20Z

Hilo1: /* NOVASEQX / NOVASEQ6000 SEQUENCING */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00
|-
| NovaSeq S4 50nt Lane**|| $3,780.00 || $4,158.00
|-
| NovaSeq S4 300nt Lane**|| $5,400.00 || $5,940.00
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00
|-
| NovaSeqX 10B 300nt^^ || $12,160.00 || $13,376.00
|-
| NovaSeqX 25B 300nt^^ || $16,200.00 || $17,820.00
|-
| NovaSeqX 25B 100nt^^ || $5,750.00 || $6,325.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

{| class="wikitable"
|-
!i100 kit!! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
|5M 300nt || $715.00 || $786.50
|-
|5M 600nt || $1,040.00 || $1,144.00
|-
|25M 100nt || $925.00 || $1,017.50
|-
|25M 300nt|| $1,334.00 || $1,467.40
|-
|25M 600nt || $1,570.00 || $1,727.00
|-
|25M 1000nt|| $2,590.00 || $2,849.00
|-
|100M 100nt || $1,540.00 || $1,694.00
|-
|100M 300nt || $2,125.00 || $2,337.50
|-
|50M 600nt || $2,125.00 || $2,337.50
|}

==== MiSeq i100 Walkup ====
Training and lab storage on BMC servers required. Run must be scheduled in the [https://mit.ilabsolutions.com/schedules/555535#/schedule/ iLabs calendar.]
{| class="wikitable"
|-
!i100 Kit!! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime
|-
| Machine usage || $120.00 || $132.00 || Xh
|-
|5M 300nt|| $381.00 || $419.10 || 8h
|-
|5M 600nt || $665.00 || $731.50 || 16h
|-
|25M 100nt || $565.00 || $621.50 || 5h
|-
|25M 300nt || $920.00 || $1,012.00 || 8h
|-
|25M 600nt || $1,125.00 || $1,238.00 || 16h
|-
|25M 1000nt || $1,921.00 || $2,113.10 || 24h
|-
|100M 100nt || $1,130.00 || $1,243.00 || 5h
|-
|100M 300nt || $1,638.50 || $1,802.35 || 8h
|-
|50M 600nt || $1,638.50 || $1,802.35 || 16h
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| 70nt V2 || $1,320.00 || $1,452.00
|-
| 150nt V3 || $1,430.00 || $1,573.00
|-
| 300nt V2 || $1,595.00 || $1,754.50
|-
| 500nt V2 || $1,760.00 || $1,936.00
|-
| 600nt V3 || $2,200.00 || $2,420.00
|-
| 500nt '''NANO''' Run || $770.00 || $847.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples submitted not in a plated format will be subject to these additional charges.
|-
| High Throughput Setup || STD || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || STD || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || STD || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" |'''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || STD || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || STD || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || STD v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || HTL (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || HTL (NEB only) || 96 || $3,000.00 || $3,300.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || HTL || 96 || $2,100.00 || $2,310.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00
|-
| || || 96 || $3,200.00 || $3,520.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || STD || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || STD || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || STD || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Standard Ligation || LSK || $160.00 || $176.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00
|-
| Direct RNA || RNA004 || $160.00 || $176.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| DNA || 96 || $700.00 || $770.00
|-
| RNA || 96 || $800.00 || $880.00
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $200.00 || $220.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Slide Only || per slide || $150.00 || $165.00
|-
| Optimization Only || per slide || $800.00 || $880.00
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00
|-
| Antibody Add-on || per slide || $360.00 || $396.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00
|-
| FFPE Curl || per curl || $60.00 || $66.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $300.00 || $330.00 ||
|-
| || add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

Email biomicro@mit.edu for training

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! iLabs
|-
| Roche LightCycler 480 || $15.00 || per plate ||[https://mit.ilabsolutions.com/schedules/261680#/schedule/ ilab qPCR calendar]
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || [https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|-
| 96-well PLATES || $10.00 || per plate ||[https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $18.00 / $20.00 || per hour || [https://mit.ilabsolutions.com/schedules/262715#/schedule/ ilabs plate reader calendar] || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''ROBOTICS'''
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || [https://mit.ilabsolutions.com/service_center/3381/?tab=services services request]
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-15T18:50:12Z

Hilo1: /* NOVASEQX / NOVASEQ6000 SEQUENCING */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !!
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00
|-
| NovaSeq S4 50nt Lane**|| $3,780.00 || $4,158.00
|-
| NovaSeq S4 300nt Lane**|| $5,400.00 || $5,940.00
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00
|-
| NovaSeqX 10B 300nt^^ || $12,160.00 || $13,376.00
|-
| NovaSeqX 25B 300nt^^ || $16,200.00 || $17,820.00
|-
| NovaSeqX 25B 100nt^^ || $5,750.00 || $6,325.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

{| class="wikitable"
|-
!i100 kit!! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
|5M 300nt || $715.00 || $786.50
|-
|5M 600nt || $1,040.00 || $1,144.00
|-
|25M 100nt || $925.00 || $1,017.50
|-
|25M 300nt|| $1,334.00 || $1,467.40
|-
|25M 600nt || $1,570.00 || $1,727.00
|-
|25M 1000nt|| $2,590.00 || $2,849.00
|-
|100M 100nt || $1,540.00 || $1,694.00
|-
|100M 300nt || $2,125.00 || $2,337.50
|-
|50M 600nt || $2,125.00 || $2,337.50
|}

==== MiSeq i100 Walkup ====
Training and lab storage on BMC servers required. Run must be scheduled in the [https://mit.ilabsolutions.com/schedules/555535#/schedule/ iLabs calendar.]
{| class="wikitable"
|-
!i100 Kit!! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime
|-
| Machine usage || $120.00 || $132.00 || Xh
|-
|5M 300nt|| $381.00 || $419.10 || 8h
|-
|5M 600nt || $665.00 || $731.50 || 16h
|-
|25M 100nt || $565.00 || $621.50 || 5h
|-
|25M 300nt || $920.00 || $1,012.00 || 8h
|-
|25M 600nt || $1,125.00 || $1,238.00 || 16h
|-
|25M 1000nt || $1,921.00 || $2,113.10 || 24h
|-
|100M 100nt || $1,130.00 || $1,243.00 || 5h
|-
|100M 300nt || $1,638.50 || $1,802.35 || 8h
|-
|50M 600nt || $1,638.50 || $1,802.35 || 16h
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| 70nt V2 || $1,320.00 || $1,452.00
|-
| 150nt V3 || $1,430.00 || $1,573.00
|-
| 300nt V2 || $1,595.00 || $1,754.50
|-
| 500nt V2 || $1,760.00 || $1,936.00
|-
| 600nt V3 || $2,200.00 || $2,420.00
|-
| 500nt '''NANO''' Run || $770.00 || $847.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples submitted not in a plated format will be subject to these additional charges.
|-
| High Throughput Setup || STD || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || STD || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || STD || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" |'''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || STD || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || STD || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || STD v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || HTL (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || HTL (NEB only) || 96 || $3,000.00 || $3,300.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || HTL || 96 || $2,100.00 || $2,310.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00
|-
| || || 96 || $3,200.00 || $3,520.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || STD || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || STD || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || STD || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Standard Ligation || LSK || $160.00 || $176.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00
|-
| Direct RNA || RNA004 || $160.00 || $176.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| DNA || 96 || $700.00 || $770.00
|-
| RNA || 96 || $800.00 || $880.00
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $200.00 || $220.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Slide Only || per slide || $150.00 || $165.00
|-
| Optimization Only || per slide || $800.00 || $880.00
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00
|-
| Antibody Add-on || per slide || $360.00 || $396.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00
|-
| FFPE Curl || per curl || $60.00 || $66.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $300.00 || $330.00 ||
|-
| || add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

Email biomicro@mit.edu for training

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! iLabs
|-
| Roche LightCycler 480 || $15.00 || per plate ||[https://mit.ilabsolutions.com/schedules/261680#/schedule/ ilab qPCR calendar]
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || [https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|-
| 96-well PLATES || $10.00 || per plate ||[https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $18.00 / $20.00 || per hour || [https://mit.ilabsolutions.com/schedules/262715#/schedule/ ilabs plate reader calendar] || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''ROBOTICS'''
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || [https://mit.ilabsolutions.com/service_center/3381/?tab=services services request]
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-15T18:49:11Z

Hilo1: /* SHORT READ LIBRARY - RNA */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90 || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00
|-
| NovaSeq S4 50nt Lane**|| $3,780.00 || $4,158.00 || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq S4 300nt Lane**|| $5,400.00 || $5,940.00
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00 || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00
|-
| NovaSeqX 10B 300nt^^ || $12,160.00 || $13,376.00
|-
| NovaSeqX 25B 300nt^^ || $16,200.00 || $17,820.00
|-
| NovaSeqX 25B 100nt^^ || $5,750.00 || $6,325.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

{| class="wikitable"
|-
!i100 kit!! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
|5M 300nt || $715.00 || $786.50
|-
|5M 600nt || $1,040.00 || $1,144.00
|-
|25M 100nt || $925.00 || $1,017.50
|-
|25M 300nt|| $1,334.00 || $1,467.40
|-
|25M 600nt || $1,570.00 || $1,727.00
|-
|25M 1000nt|| $2,590.00 || $2,849.00
|-
|100M 100nt || $1,540.00 || $1,694.00
|-
|100M 300nt || $2,125.00 || $2,337.50
|-
|50M 600nt || $2,125.00 || $2,337.50
|}

==== MiSeq i100 Walkup ====
Training and lab storage on BMC servers required. Run must be scheduled in the [https://mit.ilabsolutions.com/schedules/555535#/schedule/ iLabs calendar.]
{| class="wikitable"
|-
!i100 Kit!! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime
|-
| Machine usage || $120.00 || $132.00 || Xh
|-
|5M 300nt|| $381.00 || $419.10 || 8h
|-
|5M 600nt || $665.00 || $731.50 || 16h
|-
|25M 100nt || $565.00 || $621.50 || 5h
|-
|25M 300nt || $920.00 || $1,012.00 || 8h
|-
|25M 600nt || $1,125.00 || $1,238.00 || 16h
|-
|25M 1000nt || $1,921.00 || $2,113.10 || 24h
|-
|100M 100nt || $1,130.00 || $1,243.00 || 5h
|-
|100M 300nt || $1,638.50 || $1,802.35 || 8h
|-
|50M 600nt || $1,638.50 || $1,802.35 || 16h
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| 70nt V2 || $1,320.00 || $1,452.00
|-
| 150nt V3 || $1,430.00 || $1,573.00
|-
| 300nt V2 || $1,595.00 || $1,754.50
|-
| 500nt V2 || $1,760.00 || $1,936.00
|-
| 600nt V3 || $2,200.00 || $2,420.00
|-
| 500nt '''NANO''' Run || $770.00 || $847.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples submitted not in a plated format will be subject to these additional charges.
|-
| High Throughput Setup || STD || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || STD || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || STD || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" |'''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || STD || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || STD || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || STD v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || HTL (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || HTL (NEB only) || 96 || $3,000.00 || $3,300.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || HTL || 96 || $2,100.00 || $2,310.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00
|-
| || || 96 || $3,200.00 || $3,520.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || STD || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || STD || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || STD || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Standard Ligation || LSK || $160.00 || $176.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00
|-
| Direct RNA || RNA004 || $160.00 || $176.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| DNA || 96 || $700.00 || $770.00
|-
| RNA || 96 || $800.00 || $880.00
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $200.00 || $220.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Slide Only || per slide || $150.00 || $165.00
|-
| Optimization Only || per slide || $800.00 || $880.00
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00
|-
| Antibody Add-on || per slide || $360.00 || $396.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00
|-
| FFPE Curl || per curl || $60.00 || $66.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $300.00 || $330.00 ||
|-
| || add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

Email biomicro@mit.edu for training

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! iLabs
|-
| Roche LightCycler 480 || $15.00 || per plate ||[https://mit.ilabsolutions.com/schedules/261680#/schedule/ ilab qPCR calendar]
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || [https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|-
| 96-well PLATES || $10.00 || per plate ||[https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $18.00 / $20.00 || per hour || [https://mit.ilabsolutions.com/schedules/262715#/schedule/ ilabs plate reader calendar] || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''ROBOTICS'''
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || [https://mit.ilabsolutions.com/service_center/3381/?tab=services services request]
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-15T18:47:47Z

Hilo1: /* SHORT READ LIBRARY - RNA */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90 || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00
|-
| NovaSeq S4 50nt Lane**|| $3,780.00 || $4,158.00 || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq S4 300nt Lane**|| $5,400.00 || $5,940.00
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00 || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00
|-
| NovaSeqX 10B 300nt^^ || $12,160.00 || $13,376.00
|-
| NovaSeqX 25B 300nt^^ || $16,200.00 || $17,820.00
|-
| NovaSeqX 25B 100nt^^ || $5,750.00 || $6,325.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

{| class="wikitable"
|-
!i100 kit!! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
|5M 300nt || $715.00 || $786.50
|-
|5M 600nt || $1,040.00 || $1,144.00
|-
|25M 100nt || $925.00 || $1,017.50
|-
|25M 300nt|| $1,334.00 || $1,467.40
|-
|25M 600nt || $1,570.00 || $1,727.00
|-
|25M 1000nt|| $2,590.00 || $2,849.00
|-
|100M 100nt || $1,540.00 || $1,694.00
|-
|100M 300nt || $2,125.00 || $2,337.50
|-
|50M 600nt || $2,125.00 || $2,337.50
|}

==== MiSeq i100 Walkup ====
Training and lab storage on BMC servers required. Run must be scheduled in the [https://mit.ilabsolutions.com/schedules/555535#/schedule/ iLabs calendar.]
{| class="wikitable"
|-
!i100 Kit!! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime
|-
| Machine usage || $120.00 || $132.00 || Xh
|-
|5M 300nt|| $381.00 || $419.10 || 8h
|-
|5M 600nt || $665.00 || $731.50 || 16h
|-
|25M 100nt || $565.00 || $621.50 || 5h
|-
|25M 300nt || $920.00 || $1,012.00 || 8h
|-
|25M 600nt || $1,125.00 || $1,238.00 || 16h
|-
|25M 1000nt || $1,921.00 || $2,113.10 || 24h
|-
|100M 100nt || $1,130.00 || $1,243.00 || 5h
|-
|100M 300nt || $1,638.50 || $1,802.35 || 8h
|-
|50M 600nt || $1,638.50 || $1,802.35 || 16h
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| 70nt V2 || $1,320.00 || $1,452.00
|-
| 150nt V3 || $1,430.00 || $1,573.00
|-
| 300nt V2 || $1,595.00 || $1,754.50
|-
| 500nt V2 || $1,760.00 || $1,936.00
|-
| 600nt V3 || $2,200.00 || $2,420.00
|-
| 500nt '''NANO''' Run || $770.00 || $847.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples submitted not in a plated format will be subject to these additional charges.
|-
| High Throughput Setup || STD || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || STD || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || STD || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" |'''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,000.00 || $3,300.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,100.00 || $2,310.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00
|-
| || || 96 || $3,200.00 || $3,520.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Standard Ligation || LSK || $160.00 || $176.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00
|-
| Direct RNA || RNA004 || $160.00 || $176.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| DNA || 96 || $700.00 || $770.00
|-
| RNA || 96 || $800.00 || $880.00
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $200.00 || $220.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Slide Only || per slide || $150.00 || $165.00
|-
| Optimization Only || per slide || $800.00 || $880.00
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00
|-
| Antibody Add-on || per slide || $360.00 || $396.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00
|-
| FFPE Curl || per curl || $60.00 || $66.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $300.00 || $330.00 ||
|-
| || add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

Email biomicro@mit.edu for training

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! iLabs
|-
| Roche LightCycler 480 || $15.00 || per plate ||[https://mit.ilabsolutions.com/schedules/261680#/schedule/ ilab qPCR calendar]
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || [https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|-
| 96-well PLATES || $10.00 || per plate ||[https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $18.00 / $20.00 || per hour || [https://mit.ilabsolutions.com/schedules/262715#/schedule/ ilabs plate reader calendar] || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''ROBOTICS'''
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || [https://mit.ilabsolutions.com/service_center/3381/?tab=services services request]
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-15T18:47:36Z

Hilo1: /* SHORT READ LIBRARY - RNA */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90 || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00
|-
| NovaSeq S4 50nt Lane**|| $3,780.00 || $4,158.00 || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq S4 300nt Lane**|| $5,400.00 || $5,940.00
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00 || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00
|-
| NovaSeqX 10B 300nt^^ || $12,160.00 || $13,376.00
|-
| NovaSeqX 25B 300nt^^ || $16,200.00 || $17,820.00
|-
| NovaSeqX 25B 100nt^^ || $5,750.00 || $6,325.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

{| class="wikitable"
|-
!i100 kit!! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
|5M 300nt || $715.00 || $786.50
|-
|5M 600nt || $1,040.00 || $1,144.00
|-
|25M 100nt || $925.00 || $1,017.50
|-
|25M 300nt|| $1,334.00 || $1,467.40
|-
|25M 600nt || $1,570.00 || $1,727.00
|-
|25M 1000nt|| $2,590.00 || $2,849.00
|-
|100M 100nt || $1,540.00 || $1,694.00
|-
|100M 300nt || $2,125.00 || $2,337.50
|-
|50M 600nt || $2,125.00 || $2,337.50
|}

==== MiSeq i100 Walkup ====
Training and lab storage on BMC servers required. Run must be scheduled in the [https://mit.ilabsolutions.com/schedules/555535#/schedule/ iLabs calendar.]
{| class="wikitable"
|-
!i100 Kit!! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime
|-
| Machine usage || $120.00 || $132.00 || Xh
|-
|5M 300nt|| $381.00 || $419.10 || 8h
|-
|5M 600nt || $665.00 || $731.50 || 16h
|-
|25M 100nt || $565.00 || $621.50 || 5h
|-
|25M 300nt || $920.00 || $1,012.00 || 8h
|-
|25M 600nt || $1,125.00 || $1,238.00 || 16h
|-
|25M 1000nt || $1,921.00 || $2,113.10 || 24h
|-
|100M 100nt || $1,130.00 || $1,243.00 || 5h
|-
|100M 300nt || $1,638.50 || $1,802.35 || 8h
|-
|50M 600nt || $1,638.50 || $1,802.35 || 16h
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| 70nt V2 || $1,320.00 || $1,452.00
|-
| 150nt V3 || $1,430.00 || $1,573.00
|-
| 300nt V2 || $1,595.00 || $1,754.50
|-
| 500nt V2 || $1,760.00 || $1,936.00
|-
| 600nt V3 || $2,200.00 || $2,420.00
|-
| 500nt '''NANO''' Run || $770.00 || $847.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples submitted not in a plated format will be subject to these additional charges.
|-
| High Throughput Setup || STD || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || STD || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || STD || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
|| colspan="6" |'''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,000.00 || $3,300.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,100.00 || $2,310.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00
|-
| || || 96 || $3,200.00 || $3,520.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Standard Ligation || LSK || $160.00 || $176.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00
|-
| Direct RNA || RNA004 || $160.00 || $176.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| DNA || 96 || $700.00 || $770.00
|-
| RNA || 96 || $800.00 || $880.00
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $200.00 || $220.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Slide Only || per slide || $150.00 || $165.00
|-
| Optimization Only || per slide || $800.00 || $880.00
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00
|-
| Antibody Add-on || per slide || $360.00 || $396.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00
|-
| FFPE Curl || per curl || $60.00 || $66.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $300.00 || $330.00 ||
|-
| || add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

Email biomicro@mit.edu for training

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! iLabs
|-
| Roche LightCycler 480 || $15.00 || per plate ||[https://mit.ilabsolutions.com/schedules/261680#/schedule/ ilab qPCR calendar]
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || [https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|-
| 96-well PLATES || $10.00 || per plate ||[https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $18.00 / $20.00 || per hour || [https://mit.ilabsolutions.com/schedules/262715#/schedule/ ilabs plate reader calendar] || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''ROBOTICS'''
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || [https://mit.ilabsolutions.com/service_center/3381/?tab=services services request]
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-15T18:45:43Z

Hilo1: /* SHORT READ LIBRARY - RNA */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90 || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00
|-
| NovaSeq S4 50nt Lane**|| $3,780.00 || $4,158.00 || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq S4 300nt Lane**|| $5,400.00 || $5,940.00
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00 || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00
|-
| NovaSeqX 10B 300nt^^ || $12,160.00 || $13,376.00
|-
| NovaSeqX 25B 300nt^^ || $16,200.00 || $17,820.00
|-
| NovaSeqX 25B 100nt^^ || $5,750.00 || $6,325.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

{| class="wikitable"
|-
!i100 kit!! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
|5M 300nt || $715.00 || $786.50
|-
|5M 600nt || $1,040.00 || $1,144.00
|-
|25M 100nt || $925.00 || $1,017.50
|-
|25M 300nt|| $1,334.00 || $1,467.40
|-
|25M 600nt || $1,570.00 || $1,727.00
|-
|25M 1000nt|| $2,590.00 || $2,849.00
|-
|100M 100nt || $1,540.00 || $1,694.00
|-
|100M 300nt || $2,125.00 || $2,337.50
|-
|50M 600nt || $2,125.00 || $2,337.50
|}

==== MiSeq i100 Walkup ====
Training and lab storage on BMC servers required. Run must be scheduled in the [https://mit.ilabsolutions.com/schedules/555535#/schedule/ iLabs calendar.]
{| class="wikitable"
|-
!i100 Kit!! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime
|-
| Machine usage || $120.00 || $132.00 || Xh
|-
|5M 300nt|| $381.00 || $419.10 || 8h
|-
|5M 600nt || $665.00 || $731.50 || 16h
|-
|25M 100nt || $565.00 || $621.50 || 5h
|-
|25M 300nt || $920.00 || $1,012.00 || 8h
|-
|25M 600nt || $1,125.00 || $1,238.00 || 16h
|-
|25M 1000nt || $1,921.00 || $2,113.10 || 24h
|-
|100M 100nt || $1,130.00 || $1,243.00 || 5h
|-
|100M 300nt || $1,638.50 || $1,802.35 || 8h
|-
|50M 600nt || $1,638.50 || $1,802.35 || 16h
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| 70nt V2 || $1,320.00 || $1,452.00
|-
| 150nt V3 || $1,430.00 || $1,573.00
|-
| 300nt V2 || $1,595.00 || $1,754.50
|-
| 500nt V2 || $1,760.00 || $1,936.00
|-
| 600nt V3 || $2,200.00 || $2,420.00
|-
| 500nt '''NANO''' Run || $770.00 || $847.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples submitted not in a plated format will be subject to these additional charges.
|-
| High Throughput Setup || STD || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || STD || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || STD || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
|'''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,000.00 || $3,300.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,100.00 || $2,310.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00
|-
| || || 96 || $3,200.00 || $3,520.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Standard Ligation || LSK || $160.00 || $176.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00
|-
| Direct RNA || RNA004 || $160.00 || $176.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| DNA || 96 || $700.00 || $770.00
|-
| RNA || 96 || $800.00 || $880.00
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $200.00 || $220.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Slide Only || per slide || $150.00 || $165.00
|-
| Optimization Only || per slide || $800.00 || $880.00
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00
|-
| Antibody Add-on || per slide || $360.00 || $396.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00
|-
| FFPE Curl || per curl || $60.00 || $66.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $300.00 || $330.00 ||
|-
| || add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

Email biomicro@mit.edu for training

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! iLabs
|-
| Roche LightCycler 480 || $15.00 || per plate ||[https://mit.ilabsolutions.com/schedules/261680#/schedule/ ilab qPCR calendar]
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || [https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|-
| 96-well PLATES || $10.00 || per plate ||[https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $18.00 / $20.00 || per hour || [https://mit.ilabsolutions.com/schedules/262715#/schedule/ ilabs plate reader calendar] || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''ROBOTICS'''
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || [https://mit.ilabsolutions.com/service_center/3381/?tab=services services request]
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-15T18:45:08Z

Hilo1: /* SHORT READ LIBRARY - RNA */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90 || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00
|-
| NovaSeq S4 50nt Lane**|| $3,780.00 || $4,158.00 || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq S4 300nt Lane**|| $5,400.00 || $5,940.00
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00 || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00
|-
| NovaSeqX 10B 300nt^^ || $12,160.00 || $13,376.00
|-
| NovaSeqX 25B 300nt^^ || $16,200.00 || $17,820.00
|-
| NovaSeqX 25B 100nt^^ || $5,750.00 || $6,325.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

{| class="wikitable"
|-
!i100 kit!! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
|5M 300nt || $715.00 || $786.50
|-
|5M 600nt || $1,040.00 || $1,144.00
|-
|25M 100nt || $925.00 || $1,017.50
|-
|25M 300nt|| $1,334.00 || $1,467.40
|-
|25M 600nt || $1,570.00 || $1,727.00
|-
|25M 1000nt|| $2,590.00 || $2,849.00
|-
|100M 100nt || $1,540.00 || $1,694.00
|-
|100M 300nt || $2,125.00 || $2,337.50
|-
|50M 600nt || $2,125.00 || $2,337.50
|}

==== MiSeq i100 Walkup ====
Training and lab storage on BMC servers required. Run must be scheduled in the [https://mit.ilabsolutions.com/schedules/555535#/schedule/ iLabs calendar.]
{| class="wikitable"
|-
!i100 Kit!! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime
|-
| Machine usage || $120.00 || $132.00 || Xh
|-
|5M 300nt|| $381.00 || $419.10 || 8h
|-
|5M 600nt || $665.00 || $731.50 || 16h
|-
|25M 100nt || $565.00 || $621.50 || 5h
|-
|25M 300nt || $920.00 || $1,012.00 || 8h
|-
|25M 600nt || $1,125.00 || $1,238.00 || 16h
|-
|25M 1000nt || $1,921.00 || $2,113.10 || 24h
|-
|100M 100nt || $1,130.00 || $1,243.00 || 5h
|-
|100M 300nt || $1,638.50 || $1,802.35 || 8h
|-
|50M 600nt || $1,638.50 || $1,802.35 || 16h
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| 70nt V2 || $1,320.00 || $1,452.00
|-
| 150nt V3 || $1,430.00 || $1,573.00
|-
| 300nt V2 || $1,595.00 || $1,754.50
|-
| 500nt V2 || $1,760.00 || $1,936.00
|-
| 600nt V3 || $2,200.00 || $2,420.00
|-
| 500nt '''NANO''' Run || $770.00 || $847.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples submitted not in a plated format will be subject to these additional charges.
|-
| High Throughput Setup || STD || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || STD || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || STD || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,000.00 || $3,300.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,100.00 || $2,310.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00
|-
| || || 96 || $3,200.00 || $3,520.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Standard Ligation || LSK || $160.00 || $176.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00
|-
| Direct RNA || RNA004 || $160.00 || $176.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| DNA || 96 || $700.00 || $770.00
|-
| RNA || 96 || $800.00 || $880.00
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $200.00 || $220.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Slide Only || per slide || $150.00 || $165.00
|-
| Optimization Only || per slide || $800.00 || $880.00
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00
|-
| Antibody Add-on || per slide || $360.00 || $396.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00
|-
| FFPE Curl || per curl || $60.00 || $66.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $300.00 || $330.00 ||
|-
| || add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

Email biomicro@mit.edu for training

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! iLabs
|-
| Roche LightCycler 480 || $15.00 || per plate ||[https://mit.ilabsolutions.com/schedules/261680#/schedule/ ilab qPCR calendar]
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || [https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|-
| 96-well PLATES || $10.00 || per plate ||[https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $18.00 / $20.00 || per hour || [https://mit.ilabsolutions.com/schedules/262715#/schedule/ ilabs plate reader calendar] || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''ROBOTICS'''
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || [https://mit.ilabsolutions.com/service_center/3381/?tab=services services request]
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-15T18:43:36Z

Hilo1: /* CHEMAGIC360 */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90 || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00
|-
| NovaSeq S4 50nt Lane**|| $3,780.00 || $4,158.00 || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq S4 300nt Lane**|| $5,400.00 || $5,940.00
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00 || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00
|-
| NovaSeqX 10B 300nt^^ || $12,160.00 || $13,376.00
|-
| NovaSeqX 25B 300nt^^ || $16,200.00 || $17,820.00
|-
| NovaSeqX 25B 100nt^^ || $5,750.00 || $6,325.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

{| class="wikitable"
|-
!i100 kit!! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
|5M 300nt || $715.00 || $786.50
|-
|5M 600nt || $1,040.00 || $1,144.00
|-
|25M 100nt || $925.00 || $1,017.50
|-
|25M 300nt|| $1,334.00 || $1,467.40
|-
|25M 600nt || $1,570.00 || $1,727.00
|-
|25M 1000nt|| $2,590.00 || $2,849.00
|-
|100M 100nt || $1,540.00 || $1,694.00
|-
|100M 300nt || $2,125.00 || $2,337.50
|-
|50M 600nt || $2,125.00 || $2,337.50
|}

==== MiSeq i100 Walkup ====
Training and lab storage on BMC servers required. Run must be scheduled in the [https://mit.ilabsolutions.com/schedules/555535#/schedule/ iLabs calendar.]
{| class="wikitable"
|-
!i100 Kit!! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime
|-
| Machine usage || $120.00 || $132.00 || Xh
|-
|5M 300nt|| $381.00 || $419.10 || 8h
|-
|5M 600nt || $665.00 || $731.50 || 16h
|-
|25M 100nt || $565.00 || $621.50 || 5h
|-
|25M 300nt || $920.00 || $1,012.00 || 8h
|-
|25M 600nt || $1,125.00 || $1,238.00 || 16h
|-
|25M 1000nt || $1,921.00 || $2,113.10 || 24h
|-
|100M 100nt || $1,130.00 || $1,243.00 || 5h
|-
|100M 300nt || $1,638.50 || $1,802.35 || 8h
|-
|50M 600nt || $1,638.50 || $1,802.35 || 16h
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| 70nt V2 || $1,320.00 || $1,452.00
|-
| 150nt V3 || $1,430.00 || $1,573.00
|-
| 300nt V2 || $1,595.00 || $1,754.50
|-
| 500nt V2 || $1,760.00 || $1,936.00
|-
| 600nt V3 || $2,200.00 || $2,420.00
|-
| 500nt '''NANO''' Run || $770.00 || $847.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples submitted not in a plated format will be subject to these additional charges.
|-
| High Throughput Setup || STD || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || STD || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || STD || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,000.00 || $3,300.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,100.00 || $2,310.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00
|-
| || || 96 || $3,200.00 || $3,520.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Standard Ligation || LSK || $160.00 || $176.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00
|-
| Direct RNA || RNA004 || $160.00 || $176.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| DNA || 96 || $700.00 || $770.00
|-
| RNA || 96 || $800.00 || $880.00
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $200.00 || $220.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Slide Only || per slide || $150.00 || $165.00
|-
| Optimization Only || per slide || $800.00 || $880.00
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00
|-
| Antibody Add-on || per slide || $360.00 || $396.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00
|-
| FFPE Curl || per curl || $60.00 || $66.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $300.00 || $330.00 ||
|-
| || add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

Email biomicro@mit.edu for training

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! iLabs
|-
| Roche LightCycler 480 || $15.00 || per plate ||[https://mit.ilabsolutions.com/schedules/261680#/schedule/ ilab qPCR calendar]
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || [https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|-
| 96-well PLATES || $10.00 || per plate ||[https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $18.00 / $20.00 || per hour || [https://mit.ilabsolutions.com/schedules/262715#/schedule/ ilabs plate reader calendar] || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''ROBOTICS'''
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || [https://mit.ilabsolutions.com/service_center/3381/?tab=services services request]
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-15T18:42:55Z

Hilo1: /* SHORT READ LIBRARY - DNA */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90 || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00
|-
| NovaSeq S4 50nt Lane**|| $3,780.00 || $4,158.00 || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq S4 300nt Lane**|| $5,400.00 || $5,940.00
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00 || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00
|-
| NovaSeqX 10B 300nt^^ || $12,160.00 || $13,376.00
|-
| NovaSeqX 25B 300nt^^ || $16,200.00 || $17,820.00
|-
| NovaSeqX 25B 100nt^^ || $5,750.00 || $6,325.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

{| class="wikitable"
|-
!i100 kit!! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
|5M 300nt || $715.00 || $786.50
|-
|5M 600nt || $1,040.00 || $1,144.00
|-
|25M 100nt || $925.00 || $1,017.50
|-
|25M 300nt|| $1,334.00 || $1,467.40
|-
|25M 600nt || $1,570.00 || $1,727.00
|-
|25M 1000nt|| $2,590.00 || $2,849.00
|-
|100M 100nt || $1,540.00 || $1,694.00
|-
|100M 300nt || $2,125.00 || $2,337.50
|-
|50M 600nt || $2,125.00 || $2,337.50
|}

==== MiSeq i100 Walkup ====
Training and lab storage on BMC servers required. Run must be scheduled in the [https://mit.ilabsolutions.com/schedules/555535#/schedule/ iLabs calendar.]
{| class="wikitable"
|-
!i100 Kit!! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime
|-
| Machine usage || $120.00 || $132.00 || Xh
|-
|5M 300nt|| $381.00 || $419.10 || 8h
|-
|5M 600nt || $665.00 || $731.50 || 16h
|-
|25M 100nt || $565.00 || $621.50 || 5h
|-
|25M 300nt || $920.00 || $1,012.00 || 8h
|-
|25M 600nt || $1,125.00 || $1,238.00 || 16h
|-
|25M 1000nt || $1,921.00 || $2,113.10 || 24h
|-
|100M 100nt || $1,130.00 || $1,243.00 || 5h
|-
|100M 300nt || $1,638.50 || $1,802.35 || 8h
|-
|50M 600nt || $1,638.50 || $1,802.35 || 16h
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| 70nt V2 || $1,320.00 || $1,452.00
|-
| 150nt V3 || $1,430.00 || $1,573.00
|-
| 300nt V2 || $1,595.00 || $1,754.50
|-
| 500nt V2 || $1,760.00 || $1,936.00
|-
| 600nt V3 || $2,200.00 || $2,420.00
|-
| 500nt '''NANO''' Run || $770.00 || $847.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples submitted not in a plated format will be subject to these additional charges.
|-
| High Throughput Setup || STD || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || STD || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || STD || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,000.00 || $3,300.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,100.00 || $2,310.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00
|-
| || || 96 || $3,200.00 || $3,520.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Standard Ligation || LSK || $160.00 || $176.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00
|-
| Direct RNA || RNA004 || $160.00 || $176.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| DNA || 96 || $700.00 || $770.00 ||
|-
| RNA || 96 || $800.00 || $880.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $200.00 || $220.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Slide Only || per slide || $150.00 || $165.00
|-
| Optimization Only || per slide || $800.00 || $880.00
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00
|-
| Antibody Add-on || per slide || $360.00 || $396.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00
|-
| FFPE Curl || per curl || $60.00 || $66.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $300.00 || $330.00 ||
|-
| || add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

Email biomicro@mit.edu for training

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! iLabs
|-
| Roche LightCycler 480 || $15.00 || per plate ||[https://mit.ilabsolutions.com/schedules/261680#/schedule/ ilab qPCR calendar]
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || [https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|-
| 96-well PLATES || $10.00 || per plate ||[https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $18.00 / $20.00 || per hour || [https://mit.ilabsolutions.com/schedules/262715#/schedule/ ilabs plate reader calendar] || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''ROBOTICS'''
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || [https://mit.ilabsolutions.com/service_center/3381/?tab=services services request]
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-15T18:41:49Z

Hilo1: /* SHORT READ LIBRARY - CONVERSION */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90 || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00
|-
| NovaSeq S4 50nt Lane**|| $3,780.00 || $4,158.00 || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq S4 300nt Lane**|| $5,400.00 || $5,940.00
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00 || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00
|-
| NovaSeqX 10B 300nt^^ || $12,160.00 || $13,376.00
|-
| NovaSeqX 25B 300nt^^ || $16,200.00 || $17,820.00
|-
| NovaSeqX 25B 100nt^^ || $5,750.00 || $6,325.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

{| class="wikitable"
|-
!i100 kit!! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
|5M 300nt || $715.00 || $786.50
|-
|5M 600nt || $1,040.00 || $1,144.00
|-
|25M 100nt || $925.00 || $1,017.50
|-
|25M 300nt|| $1,334.00 || $1,467.40
|-
|25M 600nt || $1,570.00 || $1,727.00
|-
|25M 1000nt|| $2,590.00 || $2,849.00
|-
|100M 100nt || $1,540.00 || $1,694.00
|-
|100M 300nt || $2,125.00 || $2,337.50
|-
|50M 600nt || $2,125.00 || $2,337.50
|}

==== MiSeq i100 Walkup ====
Training and lab storage on BMC servers required. Run must be scheduled in the [https://mit.ilabsolutions.com/schedules/555535#/schedule/ iLabs calendar.]
{| class="wikitable"
|-
!i100 Kit!! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime
|-
| Machine usage || $120.00 || $132.00 || Xh
|-
|5M 300nt|| $381.00 || $419.10 || 8h
|-
|5M 600nt || $665.00 || $731.50 || 16h
|-
|25M 100nt || $565.00 || $621.50 || 5h
|-
|25M 300nt || $920.00 || $1,012.00 || 8h
|-
|25M 600nt || $1,125.00 || $1,238.00 || 16h
|-
|25M 1000nt || $1,921.00 || $2,113.10 || 24h
|-
|100M 100nt || $1,130.00 || $1,243.00 || 5h
|-
|100M 300nt || $1,638.50 || $1,802.35 || 8h
|-
|50M 600nt || $1,638.50 || $1,802.35 || 16h
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| 70nt V2 || $1,320.00 || $1,452.00
|-
| 150nt V3 || $1,430.00 || $1,573.00
|-
| 300nt V2 || $1,595.00 || $1,754.50
|-
| 500nt V2 || $1,760.00 || $1,936.00
|-
| 600nt V3 || $2,200.00 || $2,420.00
|-
| 500nt '''NANO''' Run || $770.00 || $847.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,000.00 || $3,300.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,100.00 || $2,310.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00
|-
| || || 96 || $3,200.00 || $3,520.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Standard Ligation || LSK || $160.00 || $176.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00
|-
| Direct RNA || RNA004 || $160.00 || $176.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| DNA || 96 || $700.00 || $770.00 ||
|-
| RNA || 96 || $800.00 || $880.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $200.00 || $220.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Slide Only || per slide || $150.00 || $165.00
|-
| Optimization Only || per slide || $800.00 || $880.00
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00
|-
| Antibody Add-on || per slide || $360.00 || $396.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00
|-
| FFPE Curl || per curl || $60.00 || $66.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $300.00 || $330.00 ||
|-
| || add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

Email biomicro@mit.edu for training

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! iLabs
|-
| Roche LightCycler 480 || $15.00 || per plate ||[https://mit.ilabsolutions.com/schedules/261680#/schedule/ ilab qPCR calendar]
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || [https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|-
| 96-well PLATES || $10.00 || per plate ||[https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $18.00 / $20.00 || per hour || [https://mit.ilabsolutions.com/schedules/262715#/schedule/ ilabs plate reader calendar] || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''ROBOTICS'''
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || [https://mit.ilabsolutions.com/service_center/3381/?tab=services services request]
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-15T18:41:23Z

Hilo1: /* SHORT READ LIBRARY - CONVERSION */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90 || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00
|-
| NovaSeq S4 50nt Lane**|| $3,780.00 || $4,158.00 || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq S4 300nt Lane**|| $5,400.00 || $5,940.00
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00 || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00
|-
| NovaSeqX 10B 300nt^^ || $12,160.00 || $13,376.00
|-
| NovaSeqX 25B 300nt^^ || $16,200.00 || $17,820.00
|-
| NovaSeqX 25B 100nt^^ || $5,750.00 || $6,325.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

{| class="wikitable"
|-
!i100 kit!! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
|5M 300nt || $715.00 || $786.50
|-
|5M 600nt || $1,040.00 || $1,144.00
|-
|25M 100nt || $925.00 || $1,017.50
|-
|25M 300nt|| $1,334.00 || $1,467.40
|-
|25M 600nt || $1,570.00 || $1,727.00
|-
|25M 1000nt|| $2,590.00 || $2,849.00
|-
|100M 100nt || $1,540.00 || $1,694.00
|-
|100M 300nt || $2,125.00 || $2,337.50
|-
|50M 600nt || $2,125.00 || $2,337.50
|}

==== MiSeq i100 Walkup ====
Training and lab storage on BMC servers required. Run must be scheduled in the [https://mit.ilabsolutions.com/schedules/555535#/schedule/ iLabs calendar.]
{| class="wikitable"
|-
!i100 Kit!! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime
|-
| Machine usage || $120.00 || $132.00 || Xh
|-
|5M 300nt|| $381.00 || $419.10 || 8h
|-
|5M 600nt || $665.00 || $731.50 || 16h
|-
|25M 100nt || $565.00 || $621.50 || 5h
|-
|25M 300nt || $920.00 || $1,012.00 || 8h
|-
|25M 600nt || $1,125.00 || $1,238.00 || 16h
|-
|25M 1000nt || $1,921.00 || $2,113.10 || 24h
|-
|100M 100nt || $1,130.00 || $1,243.00 || 5h
|-
|100M 300nt || $1,638.50 || $1,802.35 || 8h
|-
|50M 600nt || $1,638.50 || $1,802.35 || 16h
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| 70nt V2 || $1,320.00 || $1,452.00
|-
| 150nt V3 || $1,430.00 || $1,573.00
|-
| 300nt V2 || $1,595.00 || $1,754.50
|-
| 500nt V2 || $1,760.00 || $1,936.00
|-
| 600nt V3 || $2,200.00 || $2,420.00
|-
| 500nt '''NANO''' Run || $770.00 || $847.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00 || Includes QC. Samples not reindexed.
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,000.00 || $3,300.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,100.00 || $2,310.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00
|-
| || || 96 || $3,200.00 || $3,520.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Standard Ligation || LSK || $160.00 || $176.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00
|-
| Direct RNA || RNA004 || $160.00 || $176.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| DNA || 96 || $700.00 || $770.00 ||
|-
| RNA || 96 || $800.00 || $880.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $200.00 || $220.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Slide Only || per slide || $150.00 || $165.00
|-
| Optimization Only || per slide || $800.00 || $880.00
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00
|-
| Antibody Add-on || per slide || $360.00 || $396.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00
|-
| FFPE Curl || per curl || $60.00 || $66.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $300.00 || $330.00 ||
|-
| || add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

Email biomicro@mit.edu for training

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! iLabs
|-
| Roche LightCycler 480 || $15.00 || per plate ||[https://mit.ilabsolutions.com/schedules/261680#/schedule/ ilab qPCR calendar]
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || [https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|-
| 96-well PLATES || $10.00 || per plate ||[https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $18.00 / $20.00 || per hour || [https://mit.ilabsolutions.com/schedules/262715#/schedule/ ilabs plate reader calendar] || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''ROBOTICS'''
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || [https://mit.ilabsolutions.com/service_center/3381/?tab=services services request]
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-15T18:40:57Z

Hilo1: /* MiSeq classic */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90 || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00
|-
| NovaSeq S4 50nt Lane**|| $3,780.00 || $4,158.00 || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq S4 300nt Lane**|| $5,400.00 || $5,940.00
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00 || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00
|-
| NovaSeqX 10B 300nt^^ || $12,160.00 || $13,376.00
|-
| NovaSeqX 25B 300nt^^ || $16,200.00 || $17,820.00
|-
| NovaSeqX 25B 100nt^^ || $5,750.00 || $6,325.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

{| class="wikitable"
|-
!i100 kit!! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
|5M 300nt || $715.00 || $786.50
|-
|5M 600nt || $1,040.00 || $1,144.00
|-
|25M 100nt || $925.00 || $1,017.50
|-
|25M 300nt|| $1,334.00 || $1,467.40
|-
|25M 600nt || $1,570.00 || $1,727.00
|-
|25M 1000nt|| $2,590.00 || $2,849.00
|-
|100M 100nt || $1,540.00 || $1,694.00
|-
|100M 300nt || $2,125.00 || $2,337.50
|-
|50M 600nt || $2,125.00 || $2,337.50
|}

==== MiSeq i100 Walkup ====
Training and lab storage on BMC servers required. Run must be scheduled in the [https://mit.ilabsolutions.com/schedules/555535#/schedule/ iLabs calendar.]
{| class="wikitable"
|-
!i100 Kit!! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime
|-
| Machine usage || $120.00 || $132.00 || Xh
|-
|5M 300nt|| $381.00 || $419.10 || 8h
|-
|5M 600nt || $665.00 || $731.50 || 16h
|-
|25M 100nt || $565.00 || $621.50 || 5h
|-
|25M 300nt || $920.00 || $1,012.00 || 8h
|-
|25M 600nt || $1,125.00 || $1,238.00 || 16h
|-
|25M 1000nt || $1,921.00 || $2,113.10 || 24h
|-
|100M 100nt || $1,130.00 || $1,243.00 || 5h
|-
|100M 300nt || $1,638.50 || $1,802.35 || 8h
|-
|50M 600nt || $1,638.50 || $1,802.35 || 16h
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| 70nt V2 || $1,320.00 || $1,452.00
|-
| 150nt V3 || $1,430.00 || $1,573.00
|-
| 300nt V2 || $1,595.00 || $1,754.50
|-
| 500nt V2 || $1,760.00 || $1,936.00
|-
| 600nt V3 || $2,200.00 || $2,420.00
|-
| 500nt '''NANO''' Run || $770.00 || $847.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,000.00 || $3,300.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,100.00 || $2,310.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00
|-
| || || 96 || $3,200.00 || $3,520.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Standard Ligation || LSK || $160.00 || $176.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00
|-
| Direct RNA || RNA004 || $160.00 || $176.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| DNA || 96 || $700.00 || $770.00 ||
|-
| RNA || 96 || $800.00 || $880.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $200.00 || $220.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Slide Only || per slide || $150.00 || $165.00
|-
| Optimization Only || per slide || $800.00 || $880.00
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00
|-
| Antibody Add-on || per slide || $360.00 || $396.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00
|-
| FFPE Curl || per curl || $60.00 || $66.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $300.00 || $330.00 ||
|-
| || add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

Email biomicro@mit.edu for training

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! iLabs
|-
| Roche LightCycler 480 || $15.00 || per plate ||[https://mit.ilabsolutions.com/schedules/261680#/schedule/ ilab qPCR calendar]
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || [https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|-
| 96-well PLATES || $10.00 || per plate ||[https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $18.00 / $20.00 || per hour || [https://mit.ilabsolutions.com/schedules/262715#/schedule/ ilabs plate reader calendar] || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''ROBOTICS'''
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || [https://mit.ilabsolutions.com/service_center/3381/?tab=services services request]
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-15T18:40:41Z

Hilo1: /* MiSeq classic */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90 || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00
|-
| NovaSeq S4 50nt Lane**|| $3,780.00 || $4,158.00 || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq S4 300nt Lane**|| $5,400.00 || $5,940.00
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00 || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00
|-
| NovaSeqX 10B 300nt^^ || $12,160.00 || $13,376.00
|-
| NovaSeqX 25B 300nt^^ || $16,200.00 || $17,820.00
|-
| NovaSeqX 25B 100nt^^ || $5,750.00 || $6,325.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

{| class="wikitable"
|-
!i100 kit!! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
|5M 300nt || $715.00 || $786.50
|-
|5M 600nt || $1,040.00 || $1,144.00
|-
|25M 100nt || $925.00 || $1,017.50
|-
|25M 300nt|| $1,334.00 || $1,467.40
|-
|25M 600nt || $1,570.00 || $1,727.00
|-
|25M 1000nt|| $2,590.00 || $2,849.00
|-
|100M 100nt || $1,540.00 || $1,694.00
|-
|100M 300nt || $2,125.00 || $2,337.50
|-
|50M 600nt || $2,125.00 || $2,337.50
|}

==== MiSeq i100 Walkup ====
Training and lab storage on BMC servers required. Run must be scheduled in the [https://mit.ilabsolutions.com/schedules/555535#/schedule/ iLabs calendar.]
{| class="wikitable"
|-
!i100 Kit!! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime
|-
| Machine usage || $120.00 || $132.00 || Xh
|-
|5M 300nt|| $381.00 || $419.10 || 8h
|-
|5M 600nt || $665.00 || $731.50 || 16h
|-
|25M 100nt || $565.00 || $621.50 || 5h
|-
|25M 300nt || $920.00 || $1,012.00 || 8h
|-
|25M 600nt || $1,125.00 || $1,238.00 || 16h
|-
|25M 1000nt || $1,921.00 || $2,113.10 || 24h
|-
|100M 100nt || $1,130.00 || $1,243.00 || 5h
|-
|100M 300nt || $1,638.50 || $1,802.35 || 8h
|-
|50M 600nt || $1,638.50 || $1,802.35 || 16h
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ kit !! [[BioMicroCenter:CoreDeps|CORE LAB]]
|-
| 70nt V2 || $1,320.00 || $1,452.00
|-
| 150nt V3 || $1,430.00 || $1,573.00
|-
| 300nt V2 || $1,595.00 || $1,754.50
|-
| 500nt V2 || $1,760.00 || $1,936.00
|-
| 600nt V3 || $2,200.00 || $2,420.00
|-
| 500nt '''NANO''' Run || $770.00 || $847.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,000.00 || $3,300.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,100.00 || $2,310.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00
|-
| || || 96 || $3,200.00 || $3,520.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Standard Ligation || LSK || $160.00 || $176.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00
|-
| Direct RNA || RNA004 || $160.00 || $176.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| DNA || 96 || $700.00 || $770.00 ||
|-
| RNA || 96 || $800.00 || $880.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $200.00 || $220.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Slide Only || per slide || $150.00 || $165.00
|-
| Optimization Only || per slide || $800.00 || $880.00
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00
|-
| Antibody Add-on || per slide || $360.00 || $396.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00
|-
| FFPE Curl || per curl || $60.00 || $66.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $300.00 || $330.00 ||
|-
| || add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

Email biomicro@mit.edu for training

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! iLabs
|-
| Roche LightCycler 480 || $15.00 || per plate ||[https://mit.ilabsolutions.com/schedules/261680#/schedule/ ilab qPCR calendar]
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || [https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|-
| 96-well PLATES || $10.00 || per plate ||[https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $18.00 / $20.00 || per hour || [https://mit.ilabsolutions.com/schedules/262715#/schedule/ ilabs plate reader calendar] || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''ROBOTICS'''
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || [https://mit.ilabsolutions.com/service_center/3381/?tab=services services request]
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-15T18:39:31Z

Hilo1: /* MiSeq i100 Assisted */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90 || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00
|-
| NovaSeq S4 50nt Lane**|| $3,780.00 || $4,158.00 || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq S4 300nt Lane**|| $5,400.00 || $5,940.00
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00 || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00
|-
| NovaSeqX 10B 300nt^^ || $12,160.00 || $13,376.00
|-
| NovaSeqX 25B 300nt^^ || $16,200.00 || $17,820.00
|-
| NovaSeqX 25B 100nt^^ || $5,750.00 || $6,325.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

{| class="wikitable"
|-
!i100 kit!! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
|5M 300nt || $715.00 || $786.50
|-
|5M 600nt || $1,040.00 || $1,144.00
|-
|25M 100nt || $925.00 || $1,017.50
|-
|25M 300nt|| $1,334.00 || $1,467.40
|-
|25M 600nt || $1,570.00 || $1,727.00
|-
|25M 1000nt|| $2,590.00 || $2,849.00
|-
|100M 100nt || $1,540.00 || $1,694.00
|-
|100M 300nt || $2,125.00 || $2,337.50
|-
|50M 600nt || $2,125.00 || $2,337.50
|}

==== MiSeq i100 Walkup ====
Training and lab storage on BMC servers required. Run must be scheduled in the [https://mit.ilabsolutions.com/schedules/555535#/schedule/ iLabs calendar.]
{| class="wikitable"
|-
!i100 Kit!! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime
|-
| Machine usage || $120.00 || $132.00 || Xh
|-
|5M 300nt|| $381.00 || $419.10 || 8h
|-
|5M 600nt || $665.00 || $731.50 || 16h
|-
|25M 100nt || $565.00 || $621.50 || 5h
|-
|25M 300nt || $920.00 || $1,012.00 || 8h
|-
|25M 600nt || $1,125.00 || $1,238.00 || 16h
|-
|25M 1000nt || $1,921.00 || $2,113.10 || 24h
|-
|100M 100nt || $1,130.00 || $1,243.00 || 5h
|-
|100M 300nt || $1,638.50 || $1,802.35 || 8h
|-
|50M 600nt || $1,638.50 || $1,802.35 || 16h
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,320.00 || $1,452.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $1,430.00 || $1,573.00
|-
| 300nt V2 kit (eg. 150PE) || $1,595.00 || $1,754.50
|-
| 500nt V2 kit (eg. 250PE) || $1,760.00 || $1,936.00
|-
| 600nt V3 kit (eg. 300PE) || $2,200.00 || $2,420.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $770.00 || $847.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,000.00 || $3,300.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,100.00 || $2,310.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00
|-
| || || 96 || $3,200.00 || $3,520.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Standard Ligation || LSK || $160.00 || $176.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00
|-
| Direct RNA || RNA004 || $160.00 || $176.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| DNA || 96 || $700.00 || $770.00 ||
|-
| RNA || 96 || $800.00 || $880.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $200.00 || $220.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Slide Only || per slide || $150.00 || $165.00
|-
| Optimization Only || per slide || $800.00 || $880.00
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00
|-
| Antibody Add-on || per slide || $360.00 || $396.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00
|-
| FFPE Curl || per curl || $60.00 || $66.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $300.00 || $330.00 ||
|-
| || add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

Email biomicro@mit.edu for training

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! iLabs
|-
| Roche LightCycler 480 || $15.00 || per plate ||[https://mit.ilabsolutions.com/schedules/261680#/schedule/ ilab qPCR calendar]
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || [https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|-
| 96-well PLATES || $10.00 || per plate ||[https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $18.00 / $20.00 || per hour || [https://mit.ilabsolutions.com/schedules/262715#/schedule/ ilabs plate reader calendar] || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''ROBOTICS'''
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || [https://mit.ilabsolutions.com/service_center/3381/?tab=services services request]
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-15T18:39:14Z

Hilo1: /* MiSeq i100 Assisted */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90 || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00
|-
| NovaSeq S4 50nt Lane**|| $3,780.00 || $4,158.00 || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq S4 300nt Lane**|| $5,400.00 || $5,940.00
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00 || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00
|-
| NovaSeqX 10B 300nt^^ || $12,160.00 || $13,376.00
|-
| NovaSeqX 25B 300nt^^ || $16,200.00 || $17,820.00
|-
| NovaSeqX 25B 100nt^^ || $5,750.00 || $6,325.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

{| class="wikitable"
|-
!i100 kit!! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
|5M 300nt || $715.00 || $786.50
|-
|5M 600nt || $1,040.00 || $1,144.00
|-
|25M 100nt || $925.00 || $1,017.50
|-
|25M 300 nt|| $1,334.00 || $1,467.40
|-
|25M 600 nt || $1,570.00 || $1,727.00
|-
|25M '''1000nt'''|| $2,590.00 || $2,849.00
|-
|100M 100nt || $1,540.00 || $1,694.00
|-
|100M 300nt || $2,125.00 || $2,337.50
|-
|50M 600nt || $2,125.00 || $2,337.50
|}

==== MiSeq i100 Walkup ====
Training and lab storage on BMC servers required. Run must be scheduled in the [https://mit.ilabsolutions.com/schedules/555535#/schedule/ iLabs calendar.]
{| class="wikitable"
|-
!i100 Kit!! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime
|-
| Machine usage || $120.00 || $132.00 || Xh
|-
|5M 300nt|| $381.00 || $419.10 || 8h
|-
|5M 600nt || $665.00 || $731.50 || 16h
|-
|25M 100nt || $565.00 || $621.50 || 5h
|-
|25M 300nt || $920.00 || $1,012.00 || 8h
|-
|25M 600nt || $1,125.00 || $1,238.00 || 16h
|-
|25M 1000nt || $1,921.00 || $2,113.10 || 24h
|-
|100M 100nt || $1,130.00 || $1,243.00 || 5h
|-
|100M 300nt || $1,638.50 || $1,802.35 || 8h
|-
|50M 600nt || $1,638.50 || $1,802.35 || 16h
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,320.00 || $1,452.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $1,430.00 || $1,573.00
|-
| 300nt V2 kit (eg. 150PE) || $1,595.00 || $1,754.50
|-
| 500nt V2 kit (eg. 250PE) || $1,760.00 || $1,936.00
|-
| 600nt V3 kit (eg. 300PE) || $2,200.00 || $2,420.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $770.00 || $847.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,000.00 || $3,300.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,100.00 || $2,310.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00
|-
| || || 96 || $3,200.00 || $3,520.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Standard Ligation || LSK || $160.00 || $176.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00
|-
| Direct RNA || RNA004 || $160.00 || $176.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| DNA || 96 || $700.00 || $770.00 ||
|-
| RNA || 96 || $800.00 || $880.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $200.00 || $220.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Slide Only || per slide || $150.00 || $165.00
|-
| Optimization Only || per slide || $800.00 || $880.00
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00
|-
| Antibody Add-on || per slide || $360.00 || $396.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00
|-
| FFPE Curl || per curl || $60.00 || $66.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $300.00 || $330.00 ||
|-
| || add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

Email biomicro@mit.edu for training

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! iLabs
|-
| Roche LightCycler 480 || $15.00 || per plate ||[https://mit.ilabsolutions.com/schedules/261680#/schedule/ ilab qPCR calendar]
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || [https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|-
| 96-well PLATES || $10.00 || per plate ||[https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $18.00 / $20.00 || per hour || [https://mit.ilabsolutions.com/schedules/262715#/schedule/ ilabs plate reader calendar] || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''ROBOTICS'''
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || [https://mit.ilabsolutions.com/service_center/3381/?tab=services services request]
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-15T18:38:51Z

Hilo1: /* MiSeq i100 Walkup */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90 || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00
|-
| NovaSeq S4 50nt Lane**|| $3,780.00 || $4,158.00 || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq S4 300nt Lane**|| $5,400.00 || $5,940.00
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00 || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00
|-
| NovaSeqX 10B 300nt^^ || $12,160.00 || $13,376.00
|-
| NovaSeqX 25B 300nt^^ || $16,200.00 || $17,820.00
|-
| NovaSeqX 25B 100nt^^ || $5,750.00 || $6,325.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

{| class="wikitable"
|-
!Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
|5M 300nt || $715.00 || $786.50
|-
|5M 600nt || $1,040.00 || $1,144.00
|-
|25M 100nt || $925.00 || $1,017.50
|-
|25M 300 nt|| $1,334.00 || $1,467.40
|-
|25M 600 nt || $1,570.00 || $1,727.00
|-
|25M '''1000nt'''|| $2,590.00 || $2,849.00
|-
|100M 100nt || $1,540.00 || $1,694.00
|-
|100M 300nt || $2,125.00 || $2,337.50
|-
|50M 600nt || $2,125.00 || $2,337.50
|}

==== MiSeq i100 Walkup ====
Training and lab storage on BMC servers required. Run must be scheduled in the [https://mit.ilabsolutions.com/schedules/555535#/schedule/ iLabs calendar.]
{| class="wikitable"
|-
!i100 Kit!! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime
|-
| Machine usage || $120.00 || $132.00 || Xh
|-
|5M 300nt|| $381.00 || $419.10 || 8h
|-
|5M 600nt || $665.00 || $731.50 || 16h
|-
|25M 100nt || $565.00 || $621.50 || 5h
|-
|25M 300nt || $920.00 || $1,012.00 || 8h
|-
|25M 600nt || $1,125.00 || $1,238.00 || 16h
|-
|25M 1000nt || $1,921.00 || $2,113.10 || 24h
|-
|100M 100nt || $1,130.00 || $1,243.00 || 5h
|-
|100M 300nt || $1,638.50 || $1,802.35 || 8h
|-
|50M 600nt || $1,638.50 || $1,802.35 || 16h
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,320.00 || $1,452.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $1,430.00 || $1,573.00
|-
| 300nt V2 kit (eg. 150PE) || $1,595.00 || $1,754.50
|-
| 500nt V2 kit (eg. 250PE) || $1,760.00 || $1,936.00
|-
| 600nt V3 kit (eg. 300PE) || $2,200.00 || $2,420.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $770.00 || $847.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,000.00 || $3,300.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,100.00 || $2,310.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00
|-
| || || 96 || $3,200.00 || $3,520.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Standard Ligation || LSK || $160.00 || $176.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00
|-
| Direct RNA || RNA004 || $160.00 || $176.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| DNA || 96 || $700.00 || $770.00 ||
|-
| RNA || 96 || $800.00 || $880.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $200.00 || $220.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Slide Only || per slide || $150.00 || $165.00
|-
| Optimization Only || per slide || $800.00 || $880.00
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00
|-
| Antibody Add-on || per slide || $360.00 || $396.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00
|-
| FFPE Curl || per curl || $60.00 || $66.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $300.00 || $330.00 ||
|-
| || add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

Email biomicro@mit.edu for training

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! iLabs
|-
| Roche LightCycler 480 || $15.00 || per plate ||[https://mit.ilabsolutions.com/schedules/261680#/schedule/ ilab qPCR calendar]
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || [https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|-
| 96-well PLATES || $10.00 || per plate ||[https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $18.00 / $20.00 || per hour || [https://mit.ilabsolutions.com/schedules/262715#/schedule/ ilabs plate reader calendar] || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''ROBOTICS'''
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || [https://mit.ilabsolutions.com/service_center/3381/?tab=services services request]
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-15T18:32:57Z

Hilo1: /* MiSeq i100 Assisted */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90 || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00
|-
| NovaSeq S4 50nt Lane**|| $3,780.00 || $4,158.00 || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq S4 300nt Lane**|| $5,400.00 || $5,940.00
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00 || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00
|-
| NovaSeqX 10B 300nt^^ || $12,160.00 || $13,376.00
|-
| NovaSeqX 25B 300nt^^ || $16,200.00 || $17,820.00
|-
| NovaSeqX 25B 100nt^^ || $5,750.00 || $6,325.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

{| class="wikitable"
|-
!Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
|5M 300nt || $715.00 || $786.50
|-
|5M 600nt || $1,040.00 || $1,144.00
|-
|25M 100nt || $925.00 || $1,017.50
|-
|25M 300 nt|| $1,334.00 || $1,467.40
|-
|25M 600 nt || $1,570.00 || $1,727.00
|-
|25M '''1000nt'''|| $2,590.00 || $2,849.00
|-
|100M 100nt || $1,540.00 || $1,694.00
|-
|100M 300nt || $2,125.00 || $2,337.50
|-
|50M 600nt || $2,125.00 || $2,337.50
|}

==== MiSeq i100 Walkup ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime !! unit !! Notes
|-
! Length !! Reads !! !! !! !! !!
|-
| Machine usage || || $120.00 || $132.00 || || per flowcell || training and lab storage on BMC servers required.
|-
| 300nt || 5M || $381.00 || $419.10 || 8h || per kit
|-
| 600nt || 5M || $665.00 || $731.50 || 16h ||
|-
| 100nt || 25M || $565.00 || $621.50 || 5h ||
|-
| 300nt || 25M || $920.00 || $1,012.00 || 8h ||
|-
| 600nt || 25M || $1,125.00 || $1,238.00 || 16h ||
|-
| 1000nt || 25M || $1,921.00 || $2,113.10 || 24h ||
|-
| 100nt || 100M || $1,130.00 || $1,243.00 || 5h ||
|-
| 300nt || 100M || $1,638.50 || $1,802.35 || 8h ||
|-
| 600nt || 50M || $1,638.50 || $1,802.35 || 16h ||
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,320.00 || $1,452.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $1,430.00 || $1,573.00
|-
| 300nt V2 kit (eg. 150PE) || $1,595.00 || $1,754.50
|-
| 500nt V2 kit (eg. 250PE) || $1,760.00 || $1,936.00
|-
| 600nt V3 kit (eg. 300PE) || $2,200.00 || $2,420.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $770.00 || $847.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,000.00 || $3,300.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,100.00 || $2,310.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00
|-
| || || 96 || $3,200.00 || $3,520.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Standard Ligation || LSK || $160.00 || $176.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00
|-
| Direct RNA || RNA004 || $160.00 || $176.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| DNA || 96 || $700.00 || $770.00 ||
|-
| RNA || 96 || $800.00 || $880.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $200.00 || $220.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Slide Only || per slide || $150.00 || $165.00
|-
| Optimization Only || per slide || $800.00 || $880.00
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00
|-
| Antibody Add-on || per slide || $360.00 || $396.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00
|-
| FFPE Curl || per curl || $60.00 || $66.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $300.00 || $330.00 ||
|-
| || add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

Email biomicro@mit.edu for training

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! iLabs
|-
| Roche LightCycler 480 || $15.00 || per plate ||[https://mit.ilabsolutions.com/schedules/261680#/schedule/ ilab qPCR calendar]
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || [https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|-
| 96-well PLATES || $10.00 || per plate ||[https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $18.00 / $20.00 || per hour || [https://mit.ilabsolutions.com/schedules/262715#/schedule/ ilabs plate reader calendar] || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''ROBOTICS'''
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || [https://mit.ilabsolutions.com/service_center/3381/?tab=services services request]
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-15T18:32:24Z

Hilo1: /* MiSeq i100 Assisted */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90 || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00
|-
| NovaSeq S4 50nt Lane**|| $3,780.00 || $4,158.00 || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq S4 300nt Lane**|| $5,400.00 || $5,940.00
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00 || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00
|-
| NovaSeqX 10B 300nt^^ || $12,160.00 || $13,376.00
|-
| NovaSeqX 25B 300nt^^ || $16,200.00 || $17,820.00
|-
| NovaSeqX 25B 100nt^^ || $5,750.00 || $6,325.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

{| class="wikitable"
|-
!Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
|5M 300nt || $715.00 || $786.50
|-
|5M 600nt || $1,040.00 || $1,144.00
|-
|25M 100nt || $925.00 || $1,017.50
|-
|25M 300 nt|| $1,334.00 || $1,467.40
|-
|25M 600 nt || $1,570.00 || $1,727.00
|-
|25M '''1000nt'''|| $2,590.00 || $2,849.00
|-
|100M 100nt || $1,540.00 || $1,694.00
|-
|100M 300 nt || $2,125.00 || $2,337.50
|-
|50M 600 nt || $2,125.00 || $2,337.50
|}

==== MiSeq i100 Walkup ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime !! unit !! Notes
|-
! Length !! Reads !! !! !! !! !!
|-
| Machine usage || || $120.00 || $132.00 || || per flowcell || training and lab storage on BMC servers required.
|-
| 300nt || 5M || $381.00 || $419.10 || 8h || per kit
|-
| 600nt || 5M || $665.00 || $731.50 || 16h ||
|-
| 100nt || 25M || $565.00 || $621.50 || 5h ||
|-
| 300nt || 25M || $920.00 || $1,012.00 || 8h ||
|-
| 600nt || 25M || $1,125.00 || $1,238.00 || 16h ||
|-
| 1000nt || 25M || $1,921.00 || $2,113.10 || 24h ||
|-
| 100nt || 100M || $1,130.00 || $1,243.00 || 5h ||
|-
| 300nt || 100M || $1,638.50 || $1,802.35 || 8h ||
|-
| 600nt || 50M || $1,638.50 || $1,802.35 || 16h ||
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,320.00 || $1,452.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $1,430.00 || $1,573.00
|-
| 300nt V2 kit (eg. 150PE) || $1,595.00 || $1,754.50
|-
| 500nt V2 kit (eg. 250PE) || $1,760.00 || $1,936.00
|-
| 600nt V3 kit (eg. 300PE) || $2,200.00 || $2,420.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $770.00 || $847.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,000.00 || $3,300.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,100.00 || $2,310.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00
|-
| || || 96 || $3,200.00 || $3,520.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Standard Ligation || LSK || $160.00 || $176.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00
|-
| Direct RNA || RNA004 || $160.00 || $176.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| DNA || 96 || $700.00 || $770.00 ||
|-
| RNA || 96 || $800.00 || $880.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $200.00 || $220.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Slide Only || per slide || $150.00 || $165.00
|-
| Optimization Only || per slide || $800.00 || $880.00
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00
|-
| Antibody Add-on || per slide || $360.00 || $396.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00
|-
| FFPE Curl || per curl || $60.00 || $66.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $300.00 || $330.00 ||
|-
| || add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

Email biomicro@mit.edu for training

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! iLabs
|-
| Roche LightCycler 480 || $15.00 || per plate ||[https://mit.ilabsolutions.com/schedules/261680#/schedule/ ilab qPCR calendar]
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || [https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|-
| 96-well PLATES || $10.00 || per plate ||[https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $18.00 / $20.00 || per hour || [https://mit.ilabsolutions.com/schedules/262715#/schedule/ ilabs plate reader calendar] || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''ROBOTICS'''
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || [https://mit.ilabsolutions.com/service_center/3381/?tab=services services request]
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-15T18:32:02Z

Hilo1: /* MiSeq i100 Assisted */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90 || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00
|-
| NovaSeq S4 50nt Lane**|| $3,780.00 || $4,158.00 || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq S4 300nt Lane**|| $5,400.00 || $5,940.00
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00 || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00
|-
| NovaSeqX 10B 300nt^^ || $12,160.00 || $13,376.00
|-
| NovaSeqX 25B 300nt^^ || $16,200.00 || $17,820.00
|-
| NovaSeqX 25B 100nt^^ || $5,750.00 || $6,325.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

{| class="wikitable"
|-
!MISEQ i100 Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
|5M 300nt || $715.00 || $786.50
|-
|5M 600nt || $1,040.00 || $1,144.00
|-
|25M 100nt || $925.00 || $1,017.50
|-
|25M 300 nt|| $1,334.00 || $1,467.40
|-
|25M 600 nt || $1,570.00 || $1,727.00
|-
|25M '''1000nt'''|| $2,590.00 || $2,849.00
|-
|100M 100nt || $1,540.00 || $1,694.00
|-
|100M 300 nt || $2,125.00 || $2,337.50
|-
|50M 600 nt || $2,125.00 || $2,337.50
|}

==== MiSeq i100 Walkup ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime !! unit !! Notes
|-
! Length !! Reads !! !! !! !! !!
|-
| Machine usage || || $120.00 || $132.00 || || per flowcell || training and lab storage on BMC servers required.
|-
| 300nt || 5M || $381.00 || $419.10 || 8h || per kit
|-
| 600nt || 5M || $665.00 || $731.50 || 16h ||
|-
| 100nt || 25M || $565.00 || $621.50 || 5h ||
|-
| 300nt || 25M || $920.00 || $1,012.00 || 8h ||
|-
| 600nt || 25M || $1,125.00 || $1,238.00 || 16h ||
|-
| 1000nt || 25M || $1,921.00 || $2,113.10 || 24h ||
|-
| 100nt || 100M || $1,130.00 || $1,243.00 || 5h ||
|-
| 300nt || 100M || $1,638.50 || $1,802.35 || 8h ||
|-
| 600nt || 50M || $1,638.50 || $1,802.35 || 16h ||
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,320.00 || $1,452.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $1,430.00 || $1,573.00
|-
| 300nt V2 kit (eg. 150PE) || $1,595.00 || $1,754.50
|-
| 500nt V2 kit (eg. 250PE) || $1,760.00 || $1,936.00
|-
| 600nt V3 kit (eg. 300PE) || $2,200.00 || $2,420.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $770.00 || $847.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,000.00 || $3,300.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,100.00 || $2,310.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00
|-
| || || 96 || $3,200.00 || $3,520.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Standard Ligation || LSK || $160.00 || $176.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00
|-
| Direct RNA || RNA004 || $160.00 || $176.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| DNA || 96 || $700.00 || $770.00 ||
|-
| RNA || 96 || $800.00 || $880.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $200.00 || $220.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Slide Only || per slide || $150.00 || $165.00
|-
| Optimization Only || per slide || $800.00 || $880.00
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00
|-
| Antibody Add-on || per slide || $360.00 || $396.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00
|-
| FFPE Curl || per curl || $60.00 || $66.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $300.00 || $330.00 ||
|-
| || add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

Email biomicro@mit.edu for training

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! iLabs
|-
| Roche LightCycler 480 || $15.00 || per plate ||[https://mit.ilabsolutions.com/schedules/261680#/schedule/ ilab qPCR calendar]
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || [https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|-
| 96-well PLATES || $10.00 || per plate ||[https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $18.00 / $20.00 || per hour || [https://mit.ilabsolutions.com/schedules/262715#/schedule/ ilabs plate reader calendar] || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''ROBOTICS'''
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || [https://mit.ilabsolutions.com/service_center/3381/?tab=services services request]
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-15T18:31:24Z

Hilo1: /* MiSeq i100 Assisted */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90 || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00
|-
| NovaSeq S4 50nt Lane**|| $3,780.00 || $4,158.00 || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq S4 300nt Lane**|| $5,400.00 || $5,940.00
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00 || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00
|-
| NovaSeqX 10B 300nt^^ || $12,160.00 || $13,376.00
|-
| NovaSeqX 25B 300nt^^ || $16,200.00 || $17,820.00
|-
| NovaSeqX 25B 100nt^^ || $5,750.00 || $6,325.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
|5M 300nt || $715.00 || $786.50
|-
|5M 600nt || $1,040.00 || $1,144.00
|-
|25M 100nt || $925.00 || $1,017.50
|-
|25M 300 nt|| $1,334.00 || $1,467.40
|-
|25M 600 nt || $1,570.00 || $1,727.00
|-
|25M '''1000nt'''|| $2,590.00 || $2,849.00
|-
|100M 100nt || $1,540.00 || $1,694.00
|-
|100M 300 nt || $2,125.00 || $2,337.50
|-
|50M 600 nt || $2,125.00 || $2,337.50
|}

==== MiSeq i100 Walkup ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime !! unit !! Notes
|-
! Length !! Reads !! !! !! !! !!
|-
| Machine usage || || $120.00 || $132.00 || || per flowcell || training and lab storage on BMC servers required.
|-
| 300nt || 5M || $381.00 || $419.10 || 8h || per kit
|-
| 600nt || 5M || $665.00 || $731.50 || 16h ||
|-
| 100nt || 25M || $565.00 || $621.50 || 5h ||
|-
| 300nt || 25M || $920.00 || $1,012.00 || 8h ||
|-
| 600nt || 25M || $1,125.00 || $1,238.00 || 16h ||
|-
| 1000nt || 25M || $1,921.00 || $2,113.10 || 24h ||
|-
| 100nt || 100M || $1,130.00 || $1,243.00 || 5h ||
|-
| 300nt || 100M || $1,638.50 || $1,802.35 || 8h ||
|-
| 600nt || 50M || $1,638.50 || $1,802.35 || 16h ||
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,320.00 || $1,452.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $1,430.00 || $1,573.00
|-
| 300nt V2 kit (eg. 150PE) || $1,595.00 || $1,754.50
|-
| 500nt V2 kit (eg. 250PE) || $1,760.00 || $1,936.00
|-
| 600nt V3 kit (eg. 300PE) || $2,200.00 || $2,420.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $770.00 || $847.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,000.00 || $3,300.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,100.00 || $2,310.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00
|-
| || || 96 || $3,200.00 || $3,520.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Standard Ligation || LSK || $160.00 || $176.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00
|-
| Direct RNA || RNA004 || $160.00 || $176.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| DNA || 96 || $700.00 || $770.00 ||
|-
| RNA || 96 || $800.00 || $880.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $200.00 || $220.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Slide Only || per slide || $150.00 || $165.00
|-
| Optimization Only || per slide || $800.00 || $880.00
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00
|-
| Antibody Add-on || per slide || $360.00 || $396.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00
|-
| FFPE Curl || per curl || $60.00 || $66.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $300.00 || $330.00 ||
|-
| || add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

Email biomicro@mit.edu for training

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! iLabs
|-
| Roche LightCycler 480 || $15.00 || per plate ||[https://mit.ilabsolutions.com/schedules/261680#/schedule/ ilab qPCR calendar]
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || [https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|-
| 96-well PLATES || $10.00 || per plate ||[https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $18.00 / $20.00 || per hour || [https://mit.ilabsolutions.com/schedules/262715#/schedule/ ilabs plate reader calendar] || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''ROBOTICS'''
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || [https://mit.ilabsolutions.com/service_center/3381/?tab=services services request]
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-15T18:29:50Z

Hilo1: /* MiSeq i100 Assisted */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90 || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00
|-
| NovaSeq S4 50nt Lane**|| $3,780.00 || $4,158.00 || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq S4 300nt Lane**|| $5,400.00 || $5,940.00
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00 || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00
|-
| NovaSeqX 10B 300nt^^ || $12,160.00 || $13,376.00
|-
| NovaSeqX 25B 300nt^^ || $16,200.00 || $17,820.00
|-
| NovaSeqX 25B 100nt^^ || $5,750.00 || $6,325.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit
|-
|5M 300nt || $715.00 || $786.50 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
|5M 600nt || $1,040.00 || $1,144.00
|-
|25M 100nt || $925.00 || $1,017.50
|-
|25M 300 nt|| $1,334.00 || $1,467.40
|-
|25M 600 nt || $1,570.00 || $1,727.00
|-
|25M '''1000nt'''|| $2,590.00 || $2,849.00
|-
|100M 100nt || $1,540.00 || $1,694.00
|-
|100M 300 nt || $2,125.00 || $2,337.50
|-
|50M 600 nt || $2,125.00 || $2,337.50
|}

==== MiSeq i100 Walkup ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime !! unit !! Notes
|-
! Length !! Reads !! !! !! !! !!
|-
| Machine usage || || $120.00 || $132.00 || || per flowcell || training and lab storage on BMC servers required.
|-
| 300nt || 5M || $381.00 || $419.10 || 8h || per kit
|-
| 600nt || 5M || $665.00 || $731.50 || 16h ||
|-
| 100nt || 25M || $565.00 || $621.50 || 5h ||
|-
| 300nt || 25M || $920.00 || $1,012.00 || 8h ||
|-
| 600nt || 25M || $1,125.00 || $1,238.00 || 16h ||
|-
| 1000nt || 25M || $1,921.00 || $2,113.10 || 24h ||
|-
| 100nt || 100M || $1,130.00 || $1,243.00 || 5h ||
|-
| 300nt || 100M || $1,638.50 || $1,802.35 || 8h ||
|-
| 600nt || 50M || $1,638.50 || $1,802.35 || 16h ||
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,320.00 || $1,452.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $1,430.00 || $1,573.00
|-
| 300nt V2 kit (eg. 150PE) || $1,595.00 || $1,754.50
|-
| 500nt V2 kit (eg. 250PE) || $1,760.00 || $1,936.00
|-
| 600nt V3 kit (eg. 300PE) || $2,200.00 || $2,420.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $770.00 || $847.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,000.00 || $3,300.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,100.00 || $2,310.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00
|-
| || || 96 || $3,200.00 || $3,520.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Standard Ligation || LSK || $160.00 || $176.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00
|-
| Direct RNA || RNA004 || $160.00 || $176.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| DNA || 96 || $700.00 || $770.00 ||
|-
| RNA || 96 || $800.00 || $880.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $200.00 || $220.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Slide Only || per slide || $150.00 || $165.00
|-
| Optimization Only || per slide || $800.00 || $880.00
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00
|-
| Antibody Add-on || per slide || $360.00 || $396.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00
|-
| FFPE Curl || per curl || $60.00 || $66.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $300.00 || $330.00 ||
|-
| || add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

Email biomicro@mit.edu for training

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! iLabs
|-
| Roche LightCycler 480 || $15.00 || per plate ||[https://mit.ilabsolutions.com/schedules/261680#/schedule/ ilab qPCR calendar]
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || [https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|-
| 96-well PLATES || $10.00 || per plate ||[https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $18.00 / $20.00 || per hour || [https://mit.ilabsolutions.com/schedules/262715#/schedule/ ilabs plate reader calendar] || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''ROBOTICS'''
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || [https://mit.ilabsolutions.com/service_center/3381/?tab=services services request]
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-15T18:29:36Z

Hilo1: /* MiSeq i100 Assisted */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90 || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00
|-
| NovaSeq S4 50nt Lane**|| $3,780.00 || $4,158.00 || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq S4 300nt Lane**|| $5,400.00 || $5,940.00
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00 || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00
|-
| NovaSeqX 10B 300nt^^ || $12,160.00 || $13,376.00
|-
| NovaSeqX 25B 300nt^^ || $16,200.00 || $17,820.00
|-
| NovaSeqX 25B 100nt^^ || $5,750.00 || $6,325.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
|5M 300nt || $715.00 || $786.50 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
|5M 600nt || $1,040.00 || $1,144.00
|-
|25M 100nt || $925.00 || $1,017.50
|-
|25M 300 nt|| $1,334.00 || $1,467.40
|-
|25M 600 nt || $1,570.00 || $1,727.00
|-
|25M '''1000nt'''|| $2,590.00 || $2,849.00
|-
|100M 100nt || $1,540.00 || $1,694.00
|-
|100M 300 nt || $2,125.00 || $2,337.50
|-
|50M 600 nt || $2,125.00 || $2,337.50
|}

==== MiSeq i100 Walkup ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime !! unit !! Notes
|-
! Length !! Reads !! !! !! !! !!
|-
| Machine usage || || $120.00 || $132.00 || || per flowcell || training and lab storage on BMC servers required.
|-
| 300nt || 5M || $381.00 || $419.10 || 8h || per kit
|-
| 600nt || 5M || $665.00 || $731.50 || 16h ||
|-
| 100nt || 25M || $565.00 || $621.50 || 5h ||
|-
| 300nt || 25M || $920.00 || $1,012.00 || 8h ||
|-
| 600nt || 25M || $1,125.00 || $1,238.00 || 16h ||
|-
| 1000nt || 25M || $1,921.00 || $2,113.10 || 24h ||
|-
| 100nt || 100M || $1,130.00 || $1,243.00 || 5h ||
|-
| 300nt || 100M || $1,638.50 || $1,802.35 || 8h ||
|-
| 600nt || 50M || $1,638.50 || $1,802.35 || 16h ||
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,320.00 || $1,452.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $1,430.00 || $1,573.00
|-
| 300nt V2 kit (eg. 150PE) || $1,595.00 || $1,754.50
|-
| 500nt V2 kit (eg. 250PE) || $1,760.00 || $1,936.00
|-
| 600nt V3 kit (eg. 300PE) || $2,200.00 || $2,420.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $770.00 || $847.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,000.00 || $3,300.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,100.00 || $2,310.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00
|-
| || || 96 || $3,200.00 || $3,520.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Standard Ligation || LSK || $160.00 || $176.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00
|-
| Direct RNA || RNA004 || $160.00 || $176.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| DNA || 96 || $700.00 || $770.00 ||
|-
| RNA || 96 || $800.00 || $880.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $200.00 || $220.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Slide Only || per slide || $150.00 || $165.00
|-
| Optimization Only || per slide || $800.00 || $880.00
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00
|-
| Antibody Add-on || per slide || $360.00 || $396.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00
|-
| FFPE Curl || per curl || $60.00 || $66.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $300.00 || $330.00 ||
|-
| || add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

Email biomicro@mit.edu for training

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! iLabs
|-
| Roche LightCycler 480 || $15.00 || per plate ||[https://mit.ilabsolutions.com/schedules/261680#/schedule/ ilab qPCR calendar]
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || [https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|-
| 96-well PLATES || $10.00 || per plate ||[https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $18.00 / $20.00 || per hour || [https://mit.ilabsolutions.com/schedules/262715#/schedule/ ilabs plate reader calendar] || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''ROBOTICS'''
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || [https://mit.ilabsolutions.com/service_center/3381/?tab=services services request]
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-15T18:17:35Z

Hilo1: /* MiSeq i100 Assisted */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90 || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00
|-
| NovaSeq S4 50nt Lane**|| $3,780.00 || $4,158.00 || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq S4 300nt Lane**|| $5,400.00 || $5,940.00
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00 || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00
|-
| NovaSeqX 10B 300nt^^ || $12,160.00 || $13,376.00
|-
| NovaSeqX 25B 300nt^^ || $16,200.00 || $17,820.00
|-
| NovaSeqX 25B 100nt^^ || $5,750.00 || $6,325.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
! Flowcell
|-
|5M 300nt || $715.00 || $786.50 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
|5M 600nt || $1,040.00 || $1,144.00
|-
|25M 100nt || $925.00 || $1,017.50
|-
|25M 300 nt|| $1,334.00 || $1,467.40
|-
|25M 600 nt || $1,570.00 || $1,727.00
|-
|25M '''1000nt'''|| $2,590.00 || $2,849.00
|-
|100M 100nt || $1,540.00 || $1,694.00
|-
|100M 300 nt || $2,125.00 || $2,337.50
|-
|50M 600 nt || $2,125.00 || $2,337.50
|}

==== MiSeq i100 Walkup ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime !! unit !! Notes
|-
! Length !! Reads !! !! !! !! !!
|-
| Machine usage || || $120.00 || $132.00 || || per flowcell || training and lab storage on BMC servers required.
|-
| 300nt || 5M || $381.00 || $419.10 || 8h || per kit
|-
| 600nt || 5M || $665.00 || $731.50 || 16h ||
|-
| 100nt || 25M || $565.00 || $621.50 || 5h ||
|-
| 300nt || 25M || $920.00 || $1,012.00 || 8h ||
|-
| 600nt || 25M || $1,125.00 || $1,238.00 || 16h ||
|-
| 1000nt || 25M || $1,921.00 || $2,113.10 || 24h ||
|-
| 100nt || 100M || $1,130.00 || $1,243.00 || 5h ||
|-
| 300nt || 100M || $1,638.50 || $1,802.35 || 8h ||
|-
| 600nt || 50M || $1,638.50 || $1,802.35 || 16h ||
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,320.00 || $1,452.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $1,430.00 || $1,573.00
|-
| 300nt V2 kit (eg. 150PE) || $1,595.00 || $1,754.50
|-
| 500nt V2 kit (eg. 250PE) || $1,760.00 || $1,936.00
|-
| 600nt V3 kit (eg. 300PE) || $2,200.00 || $2,420.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $770.00 || $847.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,000.00 || $3,300.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,100.00 || $2,310.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00
|-
| || || 96 || $3,200.00 || $3,520.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Standard Ligation || LSK || $160.00 || $176.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00
|-
| Direct RNA || RNA004 || $160.00 || $176.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| DNA || 96 || $700.00 || $770.00 ||
|-
| RNA || 96 || $800.00 || $880.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $200.00 || $220.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Slide Only || per slide || $150.00 || $165.00
|-
| Optimization Only || per slide || $800.00 || $880.00
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00
|-
| Antibody Add-on || per slide || $360.00 || $396.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00
|-
| FFPE Curl || per curl || $60.00 || $66.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $300.00 || $330.00 ||
|-
| || add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

Email biomicro@mit.edu for training

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! iLabs
|-
| Roche LightCycler 480 || $15.00 || per plate ||[https://mit.ilabsolutions.com/schedules/261680#/schedule/ ilab qPCR calendar]
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || [https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|-
| 96-well PLATES || $10.00 || per plate ||[https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $18.00 / $20.00 || per hour || [https://mit.ilabsolutions.com/schedules/262715#/schedule/ ilabs plate reader calendar] || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''ROBOTICS'''
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || [https://mit.ilabsolutions.com/service_center/3381/?tab=services services request]
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-15T18:13:53Z

Hilo1: /* NOVASEQX / NOVASEQ6000 SEQUENCING */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90 || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00
|-
| NovaSeq S4 50nt Lane**|| $3,780.00 || $4,158.00 || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq S4 300nt Lane**|| $5,400.00 || $5,940.00
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00 || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00
|-
| NovaSeqX 10B 300nt^^ || $12,160.00 || $13,376.00
|-
| NovaSeqX 25B 300nt^^ || $16,200.00 || $17,820.00
|-
| NovaSeqX 25B 100nt^^ || $5,750.00 || $6,325.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
! Length !! Reads !! !! !! !!
|-
| 300nt || 5M || $715.00 || $786.50 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,040.00 || $1,144.00
|-
| 100nt || 25M || $925.00 || $1,017.50
|-
| 300nt || 25M || $1,334.00 || $1,467.40
|-
| 600nt || 25M || $1,570.00 || $1,727.00
|-
| '''1000nt''' || 25M || $2,590.00 || $2,849.00
|-
| 100nt || 100M || $1,540.00 || $1,694.00
|-
| 300nt || 100M || $2,125.00 || $2,337.50
|-
| 600nt || 50M || $2,125.00 || $2,337.50
|}

==== MiSeq i100 Walkup ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime !! unit !! Notes
|-
! Length !! Reads !! !! !! !! !!
|-
| Machine usage || || $120.00 || $132.00 || || per flowcell || training and lab storage on BMC servers required.
|-
| 300nt || 5M || $381.00 || $419.10 || 8h || per kit
|-
| 600nt || 5M || $665.00 || $731.50 || 16h ||
|-
| 100nt || 25M || $565.00 || $621.50 || 5h ||
|-
| 300nt || 25M || $920.00 || $1,012.00 || 8h ||
|-
| 600nt || 25M || $1,125.00 || $1,238.00 || 16h ||
|-
| 1000nt || 25M || $1,921.00 || $2,113.10 || 24h ||
|-
| 100nt || 100M || $1,130.00 || $1,243.00 || 5h ||
|-
| 300nt || 100M || $1,638.50 || $1,802.35 || 8h ||
|-
| 600nt || 50M || $1,638.50 || $1,802.35 || 16h ||
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,320.00 || $1,452.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $1,430.00 || $1,573.00
|-
| 300nt V2 kit (eg. 150PE) || $1,595.00 || $1,754.50
|-
| 500nt V2 kit (eg. 250PE) || $1,760.00 || $1,936.00
|-
| 600nt V3 kit (eg. 300PE) || $2,200.00 || $2,420.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $770.00 || $847.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,000.00 || $3,300.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,100.00 || $2,310.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00
|-
| || || 96 || $3,200.00 || $3,520.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Standard Ligation || LSK || $160.00 || $176.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00
|-
| Direct RNA || RNA004 || $160.00 || $176.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| DNA || 96 || $700.00 || $770.00 ||
|-
| RNA || 96 || $800.00 || $880.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $200.00 || $220.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Slide Only || per slide || $150.00 || $165.00
|-
| Optimization Only || per slide || $800.00 || $880.00
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00
|-
| Antibody Add-on || per slide || $360.00 || $396.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00
|-
| FFPE Curl || per curl || $60.00 || $66.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $300.00 || $330.00 ||
|-
| || add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

Email biomicro@mit.edu for training

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! iLabs
|-
| Roche LightCycler 480 || $15.00 || per plate ||[https://mit.ilabsolutions.com/schedules/261680#/schedule/ ilab qPCR calendar]
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || [https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|-
| 96-well PLATES || $10.00 || per plate ||[https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $18.00 / $20.00 || per hour || [https://mit.ilabsolutions.com/schedules/262715#/schedule/ ilabs plate reader calendar] || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''ROBOTICS'''
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || [https://mit.ilabsolutions.com/service_center/3381/?tab=services services request]
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-15T18:13:09Z

Hilo1: /* NOVASEQX / NOVASEQ6000 SEQUENCING */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90 || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00
|-
| NovaSeq S4 50nt Lane**|| $3,780.00 || $4,158.00 || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq S4 300nt Lane**|| $5,400.00 || $5,940.00
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00 || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00
|-
| NovaSeqX 10B 300nt^^ || $12,160.00 || $13,376.00
|-
| NovaSeqX 25B 300nt^^ || $16,200.00 || $17,820.00
|-
| NovaSeqX 25B 100nt^^ || $5,750.00 || $6,325.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
! Length !! Reads !! !! !! !!
|-
| 300nt || 5M || $715.00 || $786.50 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,040.00 || $1,144.00
|-
| 100nt || 25M || $925.00 || $1,017.50
|-
| 300nt || 25M || $1,334.00 || $1,467.40
|-
| 600nt || 25M || $1,570.00 || $1,727.00
|-
| '''1000nt''' || 25M || $2,590.00 || $2,849.00
|-
| 100nt || 100M || $1,540.00 || $1,694.00
|-
| 300nt || 100M || $2,125.00 || $2,337.50
|-
| 600nt || 50M || $2,125.00 || $2,337.50
|}

==== MiSeq i100 Walkup ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime !! unit !! Notes
|-
! Length !! Reads !! !! !! !! !!
|-
| Machine usage || || $120.00 || $132.00 || || per flowcell || training and lab storage on BMC servers required.
|-
| 300nt || 5M || $381.00 || $419.10 || 8h || per kit
|-
| 600nt || 5M || $665.00 || $731.50 || 16h ||
|-
| 100nt || 25M || $565.00 || $621.50 || 5h ||
|-
| 300nt || 25M || $920.00 || $1,012.00 || 8h ||
|-
| 600nt || 25M || $1,125.00 || $1,238.00 || 16h ||
|-
| 1000nt || 25M || $1,921.00 || $2,113.10 || 24h ||
|-
| 100nt || 100M || $1,130.00 || $1,243.00 || 5h ||
|-
| 300nt || 100M || $1,638.50 || $1,802.35 || 8h ||
|-
| 600nt || 50M || $1,638.50 || $1,802.35 || 16h ||
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,320.00 || $1,452.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $1,430.00 || $1,573.00
|-
| 300nt V2 kit (eg. 150PE) || $1,595.00 || $1,754.50
|-
| 500nt V2 kit (eg. 250PE) || $1,760.00 || $1,936.00
|-
| 600nt V3 kit (eg. 300PE) || $2,200.00 || $2,420.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $770.00 || $847.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,000.00 || $3,300.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,100.00 || $2,310.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00
|-
| || || 96 || $3,200.00 || $3,520.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Standard Ligation || LSK || $160.00 || $176.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00
|-
| Direct RNA || RNA004 || $160.00 || $176.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| DNA || 96 || $700.00 || $770.00 ||
|-
| RNA || 96 || $800.00 || $880.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $200.00 || $220.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Slide Only || per slide || $150.00 || $165.00
|-
| Optimization Only || per slide || $800.00 || $880.00
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00
|-
| Antibody Add-on || per slide || $360.00 || $396.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00
|-
| FFPE Curl || per curl || $60.00 || $66.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $300.00 || $330.00 ||
|-
| || add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

Email biomicro@mit.edu for training

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! iLabs
|-
| Roche LightCycler 480 || $15.00 || per plate ||[https://mit.ilabsolutions.com/schedules/261680#/schedule/ ilab qPCR calendar]
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || [https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|-
| 96-well PLATES || $10.00 || per plate ||[https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $18.00 / $20.00 || per hour || [https://mit.ilabsolutions.com/schedules/262715#/schedule/ ilabs plate reader calendar] || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''ROBOTICS'''
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || [https://mit.ilabsolutions.com/service_center/3381/?tab=services services request]
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-15T18:12:47Z

Hilo1: /* NOVASEQX / NOVASEQ6000 SEQUENCING */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90 || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00
|-
| NovaSeq S4 50nt Lane**|| $3,780.00 || $4,158.00 || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq S4 300nt Lane**|| $5,400.00 || $5,940.00
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00 || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00
|-
| NovaSeqX 10B 300nt Flowcell^^ || $12,160.00 || $13,376.00
|-
| NovaSeqX 25B 300nt Flowcell^^ || $16,200.00 || $17,820.00
|-
| NovaSeqX 25B 100nt Flowcell^^ || $5,750.00 || $6,325.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
! Length !! Reads !! !! !! !!
|-
| 300nt || 5M || $715.00 || $786.50 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,040.00 || $1,144.00
|-
| 100nt || 25M || $925.00 || $1,017.50
|-
| 300nt || 25M || $1,334.00 || $1,467.40
|-
| 600nt || 25M || $1,570.00 || $1,727.00
|-
| '''1000nt''' || 25M || $2,590.00 || $2,849.00
|-
| 100nt || 100M || $1,540.00 || $1,694.00
|-
| 300nt || 100M || $2,125.00 || $2,337.50
|-
| 600nt || 50M || $2,125.00 || $2,337.50
|}

==== MiSeq i100 Walkup ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime !! unit !! Notes
|-
! Length !! Reads !! !! !! !! !!
|-
| Machine usage || || $120.00 || $132.00 || || per flowcell || training and lab storage on BMC servers required.
|-
| 300nt || 5M || $381.00 || $419.10 || 8h || per kit
|-
| 600nt || 5M || $665.00 || $731.50 || 16h ||
|-
| 100nt || 25M || $565.00 || $621.50 || 5h ||
|-
| 300nt || 25M || $920.00 || $1,012.00 || 8h ||
|-
| 600nt || 25M || $1,125.00 || $1,238.00 || 16h ||
|-
| 1000nt || 25M || $1,921.00 || $2,113.10 || 24h ||
|-
| 100nt || 100M || $1,130.00 || $1,243.00 || 5h ||
|-
| 300nt || 100M || $1,638.50 || $1,802.35 || 8h ||
|-
| 600nt || 50M || $1,638.50 || $1,802.35 || 16h ||
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,320.00 || $1,452.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $1,430.00 || $1,573.00
|-
| 300nt V2 kit (eg. 150PE) || $1,595.00 || $1,754.50
|-
| 500nt V2 kit (eg. 250PE) || $1,760.00 || $1,936.00
|-
| 600nt V3 kit (eg. 300PE) || $2,200.00 || $2,420.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $770.00 || $847.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,000.00 || $3,300.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,100.00 || $2,310.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00
|-
| || || 96 || $3,200.00 || $3,520.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Standard Ligation || LSK || $160.00 || $176.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00
|-
| Direct RNA || RNA004 || $160.00 || $176.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| DNA || 96 || $700.00 || $770.00 ||
|-
| RNA || 96 || $800.00 || $880.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $200.00 || $220.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Slide Only || per slide || $150.00 || $165.00
|-
| Optimization Only || per slide || $800.00 || $880.00
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00
|-
| Antibody Add-on || per slide || $360.00 || $396.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00
|-
| FFPE Curl || per curl || $60.00 || $66.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $300.00 || $330.00 ||
|-
| || add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

Email biomicro@mit.edu for training

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! iLabs
|-
| Roche LightCycler 480 || $15.00 || per plate ||[https://mit.ilabsolutions.com/schedules/261680#/schedule/ ilab qPCR calendar]
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || [https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|-
| 96-well PLATES || $10.00 || per plate ||[https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $18.00 / $20.00 || per hour || [https://mit.ilabsolutions.com/schedules/262715#/schedule/ ilabs plate reader calendar] || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''ROBOTICS'''
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || [https://mit.ilabsolutions.com/service_center/3381/?tab=services services request]
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-15T18:11:21Z

Hilo1: /* NOVASEQX / NOVASEQ6000 SEQUENCING */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90 || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00
|-
| NovaSeq S4 50nt || $3,780.00 || $4,158.00 || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq S4 300nt || $5,400.00 || $5,940.00
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00 || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00
|-
| NovaSeqX 10B 300nt Flowcell^^ || $12,160.00 || $13,376.00
|-
| NovaSeqX 25B 300nt Flowcell^^ || $16,200.00 || $17,820.00
|-
| NovaSeqX 25B 100nt Flowcell^^ || $5,750.00 || $6,325.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
! Length !! Reads !! !! !! !!
|-
| 300nt || 5M || $715.00 || $786.50 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,040.00 || $1,144.00
|-
| 100nt || 25M || $925.00 || $1,017.50
|-
| 300nt || 25M || $1,334.00 || $1,467.40
|-
| 600nt || 25M || $1,570.00 || $1,727.00
|-
| '''1000nt''' || 25M || $2,590.00 || $2,849.00
|-
| 100nt || 100M || $1,540.00 || $1,694.00
|-
| 300nt || 100M || $2,125.00 || $2,337.50
|-
| 600nt || 50M || $2,125.00 || $2,337.50
|}

==== MiSeq i100 Walkup ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime !! unit !! Notes
|-
! Length !! Reads !! !! !! !! !!
|-
| Machine usage || || $120.00 || $132.00 || || per flowcell || training and lab storage on BMC servers required.
|-
| 300nt || 5M || $381.00 || $419.10 || 8h || per kit
|-
| 600nt || 5M || $665.00 || $731.50 || 16h ||
|-
| 100nt || 25M || $565.00 || $621.50 || 5h ||
|-
| 300nt || 25M || $920.00 || $1,012.00 || 8h ||
|-
| 600nt || 25M || $1,125.00 || $1,238.00 || 16h ||
|-
| 1000nt || 25M || $1,921.00 || $2,113.10 || 24h ||
|-
| 100nt || 100M || $1,130.00 || $1,243.00 || 5h ||
|-
| 300nt || 100M || $1,638.50 || $1,802.35 || 8h ||
|-
| 600nt || 50M || $1,638.50 || $1,802.35 || 16h ||
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,320.00 || $1,452.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $1,430.00 || $1,573.00
|-
| 300nt V2 kit (eg. 150PE) || $1,595.00 || $1,754.50
|-
| 500nt V2 kit (eg. 250PE) || $1,760.00 || $1,936.00
|-
| 600nt V3 kit (eg. 300PE) || $2,200.00 || $2,420.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $770.00 || $847.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,000.00 || $3,300.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,100.00 || $2,310.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00
|-
| || || 96 || $3,200.00 || $3,520.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Standard Ligation || LSK || $160.00 || $176.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00
|-
| Direct RNA || RNA004 || $160.00 || $176.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| DNA || 96 || $700.00 || $770.00 ||
|-
| RNA || 96 || $800.00 || $880.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $200.00 || $220.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Slide Only || per slide || $150.00 || $165.00
|-
| Optimization Only || per slide || $800.00 || $880.00
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00
|-
| Antibody Add-on || per slide || $360.00 || $396.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00
|-
| FFPE Curl || per curl || $60.00 || $66.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $300.00 || $330.00 ||
|-
| || add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

Email biomicro@mit.edu for training

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! iLabs
|-
| Roche LightCycler 480 || $15.00 || per plate ||[https://mit.ilabsolutions.com/schedules/261680#/schedule/ ilab qPCR calendar]
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || [https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|-
| 96-well PLATES || $10.00 || per plate ||[https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $18.00 / $20.00 || per hour || [https://mit.ilabsolutions.com/schedules/262715#/schedule/ ilabs plate reader calendar] || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''ROBOTICS'''
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || [https://mit.ilabsolutions.com/service_center/3381/?tab=services services request]
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-15T18:09:56Z

Hilo1: /* QUALITY CONTROL */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90 || Per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00
|-
| NovaSeq S4 50nt || $3,780.00 || $4,158.00 || Per Lane || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq S4 300nt || $5,400.00 || $5,940.00
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00 || Per Lane || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00
|-
| NovaSeqX 10B 300nt Flowcell^^ || $12,160.00 || $13,376.00 || Per Flowcell
|-
| NovaSeqX 25B 300nt Flowcell^^ || $16,200.00 || $17,820.00
|-
| NovaSeqX 25B 100nt Flowcell^^ || $5,750.00 || $6,325.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
! Length !! Reads !! !! !! !!
|-
| 300nt || 5M || $715.00 || $786.50 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,040.00 || $1,144.00
|-
| 100nt || 25M || $925.00 || $1,017.50
|-
| 300nt || 25M || $1,334.00 || $1,467.40
|-
| 600nt || 25M || $1,570.00 || $1,727.00
|-
| '''1000nt''' || 25M || $2,590.00 || $2,849.00
|-
| 100nt || 100M || $1,540.00 || $1,694.00
|-
| 300nt || 100M || $2,125.00 || $2,337.50
|-
| 600nt || 50M || $2,125.00 || $2,337.50
|}

==== MiSeq i100 Walkup ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime !! unit !! Notes
|-
! Length !! Reads !! !! !! !! !!
|-
| Machine usage || || $120.00 || $132.00 || || per flowcell || training and lab storage on BMC servers required.
|-
| 300nt || 5M || $381.00 || $419.10 || 8h || per kit
|-
| 600nt || 5M || $665.00 || $731.50 || 16h ||
|-
| 100nt || 25M || $565.00 || $621.50 || 5h ||
|-
| 300nt || 25M || $920.00 || $1,012.00 || 8h ||
|-
| 600nt || 25M || $1,125.00 || $1,238.00 || 16h ||
|-
| 1000nt || 25M || $1,921.00 || $2,113.10 || 24h ||
|-
| 100nt || 100M || $1,130.00 || $1,243.00 || 5h ||
|-
| 300nt || 100M || $1,638.50 || $1,802.35 || 8h ||
|-
| 600nt || 50M || $1,638.50 || $1,802.35 || 16h ||
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,320.00 || $1,452.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $1,430.00 || $1,573.00
|-
| 300nt V2 kit (eg. 150PE) || $1,595.00 || $1,754.50
|-
| 500nt V2 kit (eg. 250PE) || $1,760.00 || $1,936.00
|-
| 600nt V3 kit (eg. 300PE) || $2,200.00 || $2,420.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $770.00 || $847.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,000.00 || $3,300.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,100.00 || $2,310.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00
|-
| || || 96 || $3,200.00 || $3,520.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Standard Ligation || LSK || $160.00 || $176.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00
|-
| Direct RNA || RNA004 || $160.00 || $176.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| DNA || 96 || $700.00 || $770.00 ||
|-
| RNA || 96 || $800.00 || $880.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $200.00 || $220.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Slide Only || per slide || $150.00 || $165.00
|-
| Optimization Only || per slide || $800.00 || $880.00
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00
|-
| Antibody Add-on || per slide || $360.00 || $396.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00
|-
| FFPE Curl || per curl || $60.00 || $66.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $300.00 || $330.00 ||
|-
| || add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

Email biomicro@mit.edu for training

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! iLabs
|-
| Roche LightCycler 480 || $15.00 || per plate ||[https://mit.ilabsolutions.com/schedules/261680#/schedule/ ilab qPCR calendar]
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || [https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|-
| 96-well PLATES || $10.00 || per plate ||[https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $18.00 / $20.00 || per hour || [https://mit.ilabsolutions.com/schedules/262715#/schedule/ ilabs plate reader calendar] || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''ROBOTICS'''
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || [https://mit.ilabsolutions.com/service_center/3381/?tab=services services request]
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-15T18:09:33Z

Hilo1: /* OTHER LAB SERVICES */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90 || Per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00
|-
| NovaSeq S4 50nt || $3,780.00 || $4,158.00 || Per Lane || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq S4 300nt || $5,400.00 || $5,940.00
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00 || Per Lane || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00
|-
| NovaSeqX 10B 300nt Flowcell^^ || $12,160.00 || $13,376.00 || Per Flowcell
|-
| NovaSeqX 25B 300nt Flowcell^^ || $16,200.00 || $17,820.00
|-
| NovaSeqX 25B 100nt Flowcell^^ || $5,750.00 || $6,325.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
! Length !! Reads !! !! !! !!
|-
| 300nt || 5M || $715.00 || $786.50 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,040.00 || $1,144.00
|-
| 100nt || 25M || $925.00 || $1,017.50
|-
| 300nt || 25M || $1,334.00 || $1,467.40
|-
| 600nt || 25M || $1,570.00 || $1,727.00
|-
| '''1000nt''' || 25M || $2,590.00 || $2,849.00
|-
| 100nt || 100M || $1,540.00 || $1,694.00
|-
| 300nt || 100M || $2,125.00 || $2,337.50
|-
| 600nt || 50M || $2,125.00 || $2,337.50
|}

==== MiSeq i100 Walkup ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime !! unit !! Notes
|-
! Length !! Reads !! !! !! !! !!
|-
| Machine usage || || $120.00 || $132.00 || || per flowcell || training and lab storage on BMC servers required.
|-
| 300nt || 5M || $381.00 || $419.10 || 8h || per kit
|-
| 600nt || 5M || $665.00 || $731.50 || 16h ||
|-
| 100nt || 25M || $565.00 || $621.50 || 5h ||
|-
| 300nt || 25M || $920.00 || $1,012.00 || 8h ||
|-
| 600nt || 25M || $1,125.00 || $1,238.00 || 16h ||
|-
| 1000nt || 25M || $1,921.00 || $2,113.10 || 24h ||
|-
| 100nt || 100M || $1,130.00 || $1,243.00 || 5h ||
|-
| 300nt || 100M || $1,638.50 || $1,802.35 || 8h ||
|-
| 600nt || 50M || $1,638.50 || $1,802.35 || 16h ||
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,320.00 || $1,452.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $1,430.00 || $1,573.00
|-
| 300nt V2 kit (eg. 150PE) || $1,595.00 || $1,754.50
|-
| 500nt V2 kit (eg. 250PE) || $1,760.00 || $1,936.00
|-
| 600nt V3 kit (eg. 300PE) || $2,200.00 || $2,420.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $770.00 || $847.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,000.00 || $3,300.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,100.00 || $2,310.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00
|-
| || || 96 || $3,200.00 || $3,520.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Standard Ligation || LSK || $160.00 || $176.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00
|-
| Direct RNA || RNA004 || $160.00 || $176.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| DNA || 96 || $700.00 || $770.00 ||
|-
| RNA || 96 || $800.00 || $880.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $200.00 || $220.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Slide Only || per slide || $150.00 || $165.00
|-
| Optimization Only || per slide || $800.00 || $880.00
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00
|-
| Antibody Add-on || per slide || $360.00 || $396.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00 ||
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:''' ||
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00 ||
|-
| FFPE Curl || per curl || $60.00 || $66.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $300.00 || $330.00 ||
|-
| || add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

Email biomicro@mit.edu for training

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! iLabs
|-
| Roche LightCycler 480 || $15.00 || per plate ||[https://mit.ilabsolutions.com/schedules/261680#/schedule/ ilab qPCR calendar]
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || [https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|-
| 96-well PLATES || $10.00 || per plate ||[https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $18.00 / $20.00 || per hour || [https://mit.ilabsolutions.com/schedules/262715#/schedule/ ilabs plate reader calendar] || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''ROBOTICS'''
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || [https://mit.ilabsolutions.com/service_center/3381/?tab=services services request]
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-15T18:06:53Z

Hilo1: /* REAL TIME PCR */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90 || Per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00
|-
| NovaSeq S4 50nt || $3,780.00 || $4,158.00 || Per Lane || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq S4 300nt || $5,400.00 || $5,940.00
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00 || Per Lane || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00
|-
| NovaSeqX 10B 300nt Flowcell^^ || $12,160.00 || $13,376.00 || Per Flowcell
|-
| NovaSeqX 25B 300nt Flowcell^^ || $16,200.00 || $17,820.00
|-
| NovaSeqX 25B 100nt Flowcell^^ || $5,750.00 || $6,325.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
! Length !! Reads !! !! !! !!
|-
| 300nt || 5M || $715.00 || $786.50 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,040.00 || $1,144.00
|-
| 100nt || 25M || $925.00 || $1,017.50
|-
| 300nt || 25M || $1,334.00 || $1,467.40
|-
| 600nt || 25M || $1,570.00 || $1,727.00
|-
| '''1000nt''' || 25M || $2,590.00 || $2,849.00
|-
| 100nt || 100M || $1,540.00 || $1,694.00
|-
| 300nt || 100M || $2,125.00 || $2,337.50
|-
| 600nt || 50M || $2,125.00 || $2,337.50
|}

==== MiSeq i100 Walkup ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime !! unit !! Notes
|-
! Length !! Reads !! !! !! !! !!
|-
| Machine usage || || $120.00 || $132.00 || || per flowcell || training and lab storage on BMC servers required.
|-
| 300nt || 5M || $381.00 || $419.10 || 8h || per kit
|-
| 600nt || 5M || $665.00 || $731.50 || 16h ||
|-
| 100nt || 25M || $565.00 || $621.50 || 5h ||
|-
| 300nt || 25M || $920.00 || $1,012.00 || 8h ||
|-
| 600nt || 25M || $1,125.00 || $1,238.00 || 16h ||
|-
| 1000nt || 25M || $1,921.00 || $2,113.10 || 24h ||
|-
| 100nt || 100M || $1,130.00 || $1,243.00 || 5h ||
|-
| 300nt || 100M || $1,638.50 || $1,802.35 || 8h ||
|-
| 600nt || 50M || $1,638.50 || $1,802.35 || 16h ||
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,320.00 || $1,452.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $1,430.00 || $1,573.00
|-
| 300nt V2 kit (eg. 150PE) || $1,595.00 || $1,754.50
|-
| 500nt V2 kit (eg. 250PE) || $1,760.00 || $1,936.00
|-
| 600nt V3 kit (eg. 300PE) || $2,200.00 || $2,420.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $770.00 || $847.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,000.00 || $3,300.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,100.00 || $2,310.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00
|-
| || || 96 || $3,200.00 || $3,520.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Standard Ligation || LSK || $160.00 || $176.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00
|-
| Direct RNA || RNA004 || $160.00 || $176.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| DNA || 96 || $700.00 || $770.00 ||
|-
| RNA || 96 || $800.00 || $880.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $200.00 || $220.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Slide Only || per slide || $150.00 || $165.00
|-
| Optimization Only || per slide || $800.00 || $880.00
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00
|-
| Antibody Add-on || per slide || $360.00 || $396.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00 ||
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:''' ||
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00 ||
|-
| FFPE Curl || per curl || $60.00 || $66.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $300.00 || $330.00 ||
|-
| || add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

Email biomicro@mit.edu for training

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! iLabs
|-
| Roche LightCycler 480 || $15.00 || per plate ||[https://mit.ilabsolutions.com/schedules/261680#/schedule/ ilab qPCR calendar]
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || [https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|-
| 96-well PLATES || $10.00 || per plate ||[https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR''' ||
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP''' ||
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA ||
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER''' ||
|-
| Varioskan || $18.00 / $20.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''ROBOTICS''' ||
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || ||
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training ||
|-
| colspan="5" | '''NANODROP''' ||
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-15T18:04:14Z

Hilo1: /* REAL TIME PCR */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90 || Per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00
|-
| NovaSeq S4 50nt || $3,780.00 || $4,158.00 || Per Lane || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq S4 300nt || $5,400.00 || $5,940.00
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00 || Per Lane || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00
|-
| NovaSeqX 10B 300nt Flowcell^^ || $12,160.00 || $13,376.00 || Per Flowcell
|-
| NovaSeqX 25B 300nt Flowcell^^ || $16,200.00 || $17,820.00
|-
| NovaSeqX 25B 100nt Flowcell^^ || $5,750.00 || $6,325.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
! Length !! Reads !! !! !! !!
|-
| 300nt || 5M || $715.00 || $786.50 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,040.00 || $1,144.00
|-
| 100nt || 25M || $925.00 || $1,017.50
|-
| 300nt || 25M || $1,334.00 || $1,467.40
|-
| 600nt || 25M || $1,570.00 || $1,727.00
|-
| '''1000nt''' || 25M || $2,590.00 || $2,849.00
|-
| 100nt || 100M || $1,540.00 || $1,694.00
|-
| 300nt || 100M || $2,125.00 || $2,337.50
|-
| 600nt || 50M || $2,125.00 || $2,337.50
|}

==== MiSeq i100 Walkup ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime !! unit !! Notes
|-
! Length !! Reads !! !! !! !! !!
|-
| Machine usage || || $120.00 || $132.00 || || per flowcell || training and lab storage on BMC servers required.
|-
| 300nt || 5M || $381.00 || $419.10 || 8h || per kit
|-
| 600nt || 5M || $665.00 || $731.50 || 16h ||
|-
| 100nt || 25M || $565.00 || $621.50 || 5h ||
|-
| 300nt || 25M || $920.00 || $1,012.00 || 8h ||
|-
| 600nt || 25M || $1,125.00 || $1,238.00 || 16h ||
|-
| 1000nt || 25M || $1,921.00 || $2,113.10 || 24h ||
|-
| 100nt || 100M || $1,130.00 || $1,243.00 || 5h ||
|-
| 300nt || 100M || $1,638.50 || $1,802.35 || 8h ||
|-
| 600nt || 50M || $1,638.50 || $1,802.35 || 16h ||
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,320.00 || $1,452.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $1,430.00 || $1,573.00
|-
| 300nt V2 kit (eg. 150PE) || $1,595.00 || $1,754.50
|-
| 500nt V2 kit (eg. 250PE) || $1,760.00 || $1,936.00
|-
| 600nt V3 kit (eg. 300PE) || $2,200.00 || $2,420.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $770.00 || $847.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,000.00 || $3,300.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,100.00 || $2,310.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00
|-
| || || 96 || $3,200.00 || $3,520.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Standard Ligation || LSK || $160.00 || $176.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00
|-
| Direct RNA || RNA004 || $160.00 || $176.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| DNA || 96 || $700.00 || $770.00 ||
|-
| RNA || 96 || $800.00 || $880.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $200.00 || $220.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Slide Only || per slide || $150.00 || $165.00
|-
| Optimization Only || per slide || $800.00 || $880.00
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00
|-
| Antibody Add-on || per slide || $360.00 || $396.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00 ||
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:''' ||
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00 ||
|-
| FFPE Curl || per curl || $60.00 || $66.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $300.00 || $330.00 ||
|-
| || add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

Email biomicro@mit.edu for training

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! iLabs
|-
| Roche LightCycler 480 || $15.00 || per plate ||
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml ||
|-
| 96-well PLATES || $10.00 || per plate ||
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR''' ||
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP''' ||
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA ||
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER''' ||
|-
| Varioskan || $18.00 / $20.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''ROBOTICS''' ||
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || ||
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training ||
|-
| colspan="5" | '''NANODROP''' ||
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-15T18:03:01Z

Hilo1: /* AVITI24 */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90 || Per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00
|-
| NovaSeq S4 50nt || $3,780.00 || $4,158.00 || Per Lane || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq S4 300nt || $5,400.00 || $5,940.00
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00 || Per Lane || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00
|-
| NovaSeqX 10B 300nt Flowcell^^ || $12,160.00 || $13,376.00 || Per Flowcell
|-
| NovaSeqX 25B 300nt Flowcell^^ || $16,200.00 || $17,820.00
|-
| NovaSeqX 25B 100nt Flowcell^^ || $5,750.00 || $6,325.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
! Length !! Reads !! !! !! !!
|-
| 300nt || 5M || $715.00 || $786.50 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,040.00 || $1,144.00
|-
| 100nt || 25M || $925.00 || $1,017.50
|-
| 300nt || 25M || $1,334.00 || $1,467.40
|-
| 600nt || 25M || $1,570.00 || $1,727.00
|-
| '''1000nt''' || 25M || $2,590.00 || $2,849.00
|-
| 100nt || 100M || $1,540.00 || $1,694.00
|-
| 300nt || 100M || $2,125.00 || $2,337.50
|-
| 600nt || 50M || $2,125.00 || $2,337.50
|}

==== MiSeq i100 Walkup ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime !! unit !! Notes
|-
! Length !! Reads !! !! !! !! !!
|-
| Machine usage || || $120.00 || $132.00 || || per flowcell || training and lab storage on BMC servers required.
|-
| 300nt || 5M || $381.00 || $419.10 || 8h || per kit
|-
| 600nt || 5M || $665.00 || $731.50 || 16h ||
|-
| 100nt || 25M || $565.00 || $621.50 || 5h ||
|-
| 300nt || 25M || $920.00 || $1,012.00 || 8h ||
|-
| 600nt || 25M || $1,125.00 || $1,238.00 || 16h ||
|-
| 1000nt || 25M || $1,921.00 || $2,113.10 || 24h ||
|-
| 100nt || 100M || $1,130.00 || $1,243.00 || 5h ||
|-
| 300nt || 100M || $1,638.50 || $1,802.35 || 8h ||
|-
| 600nt || 50M || $1,638.50 || $1,802.35 || 16h ||
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,320.00 || $1,452.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $1,430.00 || $1,573.00
|-
| 300nt V2 kit (eg. 150PE) || $1,595.00 || $1,754.50
|-
| 500nt V2 kit (eg. 250PE) || $1,760.00 || $1,936.00
|-
| 600nt V3 kit (eg. 300PE) || $2,200.00 || $2,420.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $770.00 || $847.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,000.00 || $3,300.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,100.00 || $2,310.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00
|-
| || || 96 || $3,200.00 || $3,520.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Standard Ligation || LSK || $160.00 || $176.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00
|-
| Direct RNA || RNA004 || $160.00 || $176.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| DNA || 96 || $700.00 || $770.00 ||
|-
| RNA || 96 || $800.00 || $880.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $200.00 || $220.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
| Slide Only || per slide || $150.00 || $165.00
|-
| Optimization Only || per slide || $800.00 || $880.00
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00
|-
| Antibody Add-on || per slide || $360.00 || $396.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00 ||
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:''' ||
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00 ||
|-
| FFPE Curl || per curl || $60.00 || $66.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $300.00 || $330.00 ||
|-
| || add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| Roche LightCycler 480 || $15.00 || per plate || [[BioMicroCenter:Forms#Calenders|CALENDAR]] *available for CORE only || Email biomicro@mit.edu for training
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || || Available for MIT only
|-
| 96-well PLATES || $10.00 || per plate || || Available for MIT only
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR''' ||
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP''' ||
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA ||
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER''' ||
|-
| Varioskan || $18.00 / $20.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''ROBOTICS''' ||
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || ||
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training ||
|-
| colspan="5" | '''NANODROP''' ||
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-15T18:02:48Z

Hilo1: /* 10X CHROMIUM */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90 || Per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00
|-
| NovaSeq S4 50nt || $3,780.00 || $4,158.00 || Per Lane || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq S4 300nt || $5,400.00 || $5,940.00
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00 || Per Lane || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00
|-
| NovaSeqX 10B 300nt Flowcell^^ || $12,160.00 || $13,376.00 || Per Flowcell
|-
| NovaSeqX 25B 300nt Flowcell^^ || $16,200.00 || $17,820.00
|-
| NovaSeqX 25B 100nt Flowcell^^ || $5,750.00 || $6,325.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
! Length !! Reads !! !! !! !!
|-
| 300nt || 5M || $715.00 || $786.50 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,040.00 || $1,144.00
|-
| 100nt || 25M || $925.00 || $1,017.50
|-
| 300nt || 25M || $1,334.00 || $1,467.40
|-
| 600nt || 25M || $1,570.00 || $1,727.00
|-
| '''1000nt''' || 25M || $2,590.00 || $2,849.00
|-
| 100nt || 100M || $1,540.00 || $1,694.00
|-
| 300nt || 100M || $2,125.00 || $2,337.50
|-
| 600nt || 50M || $2,125.00 || $2,337.50
|}

==== MiSeq i100 Walkup ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime !! unit !! Notes
|-
! Length !! Reads !! !! !! !! !!
|-
| Machine usage || || $120.00 || $132.00 || || per flowcell || training and lab storage on BMC servers required.
|-
| 300nt || 5M || $381.00 || $419.10 || 8h || per kit
|-
| 600nt || 5M || $665.00 || $731.50 || 16h ||
|-
| 100nt || 25M || $565.00 || $621.50 || 5h ||
|-
| 300nt || 25M || $920.00 || $1,012.00 || 8h ||
|-
| 600nt || 25M || $1,125.00 || $1,238.00 || 16h ||
|-
| 1000nt || 25M || $1,921.00 || $2,113.10 || 24h ||
|-
| 100nt || 100M || $1,130.00 || $1,243.00 || 5h ||
|-
| 300nt || 100M || $1,638.50 || $1,802.35 || 8h ||
|-
| 600nt || 50M || $1,638.50 || $1,802.35 || 16h ||
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,320.00 || $1,452.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $1,430.00 || $1,573.00
|-
| 300nt V2 kit (eg. 150PE) || $1,595.00 || $1,754.50
|-
| 500nt V2 kit (eg. 250PE) || $1,760.00 || $1,936.00
|-
| 600nt V3 kit (eg. 300PE) || $2,200.00 || $2,420.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $770.00 || $847.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,000.00 || $3,300.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,100.00 || $2,310.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00
|-
| || || 96 || $3,200.00 || $3,520.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Standard Ligation || LSK || $160.00 || $176.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00
|-
| Direct RNA || RNA004 || $160.00 || $176.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| DNA || 96 || $700.00 || $770.00 ||
|-
| RNA || 96 || $800.00 || $880.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $200.00 || $220.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Slide Only || per slide || $150.00 || $165.00 ||
|-
| Optimization Only || per slide || $800.00 || $880.00 ||
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00 ||
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00 ||
|-
| Antibody Add-on || per slide || $360.00 || $396.00 ||
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00 ||
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:''' ||
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00 ||
|-
| FFPE Curl || per curl || $60.00 || $66.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $300.00 || $330.00 ||
|-
| || add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| Roche LightCycler 480 || $15.00 || per plate || [[BioMicroCenter:Forms#Calenders|CALENDAR]] *available for CORE only || Email biomicro@mit.edu for training
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || || Available for MIT only
|-
| 96-well PLATES || $10.00 || per plate || || Available for MIT only
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR''' ||
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP''' ||
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA ||
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER''' ||
|-
| Varioskan || $18.00 / $20.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''ROBOTICS''' ||
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || ||
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training ||
|-
| colspan="5" | '''NANODROP''' ||
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-15T18:02:19Z

Hilo1: /* LONG READ LIBRARIES */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90 || Per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00
|-
| NovaSeq S4 50nt || $3,780.00 || $4,158.00 || Per Lane || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq S4 300nt || $5,400.00 || $5,940.00
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00 || Per Lane || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00
|-
| NovaSeqX 10B 300nt Flowcell^^ || $12,160.00 || $13,376.00 || Per Flowcell
|-
| NovaSeqX 25B 300nt Flowcell^^ || $16,200.00 || $17,820.00
|-
| NovaSeqX 25B 100nt Flowcell^^ || $5,750.00 || $6,325.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
! Length !! Reads !! !! !! !!
|-
| 300nt || 5M || $715.00 || $786.50 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,040.00 || $1,144.00
|-
| 100nt || 25M || $925.00 || $1,017.50
|-
| 300nt || 25M || $1,334.00 || $1,467.40
|-
| 600nt || 25M || $1,570.00 || $1,727.00
|-
| '''1000nt''' || 25M || $2,590.00 || $2,849.00
|-
| 100nt || 100M || $1,540.00 || $1,694.00
|-
| 300nt || 100M || $2,125.00 || $2,337.50
|-
| 600nt || 50M || $2,125.00 || $2,337.50
|}

==== MiSeq i100 Walkup ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime !! unit !! Notes
|-
! Length !! Reads !! !! !! !! !!
|-
| Machine usage || || $120.00 || $132.00 || || per flowcell || training and lab storage on BMC servers required.
|-
| 300nt || 5M || $381.00 || $419.10 || 8h || per kit
|-
| 600nt || 5M || $665.00 || $731.50 || 16h ||
|-
| 100nt || 25M || $565.00 || $621.50 || 5h ||
|-
| 300nt || 25M || $920.00 || $1,012.00 || 8h ||
|-
| 600nt || 25M || $1,125.00 || $1,238.00 || 16h ||
|-
| 1000nt || 25M || $1,921.00 || $2,113.10 || 24h ||
|-
| 100nt || 100M || $1,130.00 || $1,243.00 || 5h ||
|-
| 300nt || 100M || $1,638.50 || $1,802.35 || 8h ||
|-
| 600nt || 50M || $1,638.50 || $1,802.35 || 16h ||
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,320.00 || $1,452.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $1,430.00 || $1,573.00
|-
| 300nt V2 kit (eg. 150PE) || $1,595.00 || $1,754.50
|-
| 500nt V2 kit (eg. 250PE) || $1,760.00 || $1,936.00
|-
| 600nt V3 kit (eg. 300PE) || $2,200.00 || $2,420.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $770.00 || $847.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,000.00 || $3,300.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,100.00 || $2,310.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00
|-
| || || 96 || $3,200.00 || $3,520.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Standard Ligation || LSK || $160.00 || $176.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00
|-
| Direct RNA || RNA004 || $160.00 || $176.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| DNA || 96 || $700.00 || $770.00 ||
|-
| RNA || 96 || $800.00 || $880.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service''' ||
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION'' ||
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00 ||
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq'' ||
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)'' ||
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION'' ||
|-
| per session || <24h notice || $200.00 || $220.00 ||
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Slide Only || per slide || $150.00 || $165.00 ||
|-
| Optimization Only || per slide || $800.00 || $880.00 ||
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00 ||
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00 ||
|-
| Antibody Add-on || per slide || $360.00 || $396.00 ||
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00 ||
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:''' ||
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00 ||
|-
| FFPE Curl || per curl || $60.00 || $66.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $300.00 || $330.00 ||
|-
| || add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| Roche LightCycler 480 || $15.00 || per plate || [[BioMicroCenter:Forms#Calenders|CALENDAR]] *available for CORE only || Email biomicro@mit.edu for training
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || || Available for MIT only
|-
| 96-well PLATES || $10.00 || per plate || || Available for MIT only
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR''' ||
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP''' ||
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA ||
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER''' ||
|-
| Varioskan || $18.00 / $20.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''ROBOTICS''' ||
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || ||
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training ||
|-
| colspan="5" | '''NANODROP''' ||
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-15T18:01:57Z

Hilo1: /* SHORT READ LIBRARY - RNA */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90 || Per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00
|-
| NovaSeq S4 50nt || $3,780.00 || $4,158.00 || Per Lane || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq S4 300nt || $5,400.00 || $5,940.00
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00 || Per Lane || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00
|-
| NovaSeqX 10B 300nt Flowcell^^ || $12,160.00 || $13,376.00 || Per Flowcell
|-
| NovaSeqX 25B 300nt Flowcell^^ || $16,200.00 || $17,820.00
|-
| NovaSeqX 25B 100nt Flowcell^^ || $5,750.00 || $6,325.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
! Length !! Reads !! !! !! !!
|-
| 300nt || 5M || $715.00 || $786.50 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,040.00 || $1,144.00
|-
| 100nt || 25M || $925.00 || $1,017.50
|-
| 300nt || 25M || $1,334.00 || $1,467.40
|-
| 600nt || 25M || $1,570.00 || $1,727.00
|-
| '''1000nt''' || 25M || $2,590.00 || $2,849.00
|-
| 100nt || 100M || $1,540.00 || $1,694.00
|-
| 300nt || 100M || $2,125.00 || $2,337.50
|-
| 600nt || 50M || $2,125.00 || $2,337.50
|}

==== MiSeq i100 Walkup ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime !! unit !! Notes
|-
! Length !! Reads !! !! !! !! !!
|-
| Machine usage || || $120.00 || $132.00 || || per flowcell || training and lab storage on BMC servers required.
|-
| 300nt || 5M || $381.00 || $419.10 || 8h || per kit
|-
| 600nt || 5M || $665.00 || $731.50 || 16h ||
|-
| 100nt || 25M || $565.00 || $621.50 || 5h ||
|-
| 300nt || 25M || $920.00 || $1,012.00 || 8h ||
|-
| 600nt || 25M || $1,125.00 || $1,238.00 || 16h ||
|-
| 1000nt || 25M || $1,921.00 || $2,113.10 || 24h ||
|-
| 100nt || 100M || $1,130.00 || $1,243.00 || 5h ||
|-
| 300nt || 100M || $1,638.50 || $1,802.35 || 8h ||
|-
| 600nt || 50M || $1,638.50 || $1,802.35 || 16h ||
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,320.00 || $1,452.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $1,430.00 || $1,573.00
|-
| 300nt V2 kit (eg. 150PE) || $1,595.00 || $1,754.50
|-
| 500nt V2 kit (eg. 250PE) || $1,760.00 || $1,936.00
|-
| 600nt V3 kit (eg. 300PE) || $2,200.00 || $2,420.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $770.00 || $847.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,000.00 || $3,300.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,100.00 || $2,310.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00
|-
| || || 96 || $3,200.00 || $3,520.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Standard Ligation || LSK || $160.00 || $176.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00 ||
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00 ||
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00 ||
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00 ||
|-
| Direct RNA || RNA004 || $160.00 || $176.00 ||
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| DNA || 96 || $700.00 || $770.00 ||
|-
| RNA || 96 || $800.00 || $880.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service''' ||
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION'' ||
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00 ||
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq'' ||
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)'' ||
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION'' ||
|-
| per session || <24h notice || $200.00 || $220.00 ||
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Slide Only || per slide || $150.00 || $165.00 ||
|-
| Optimization Only || per slide || $800.00 || $880.00 ||
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00 ||
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00 ||
|-
| Antibody Add-on || per slide || $360.00 || $396.00 ||
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00 ||
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:''' ||
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00 ||
|-
| FFPE Curl || per curl || $60.00 || $66.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $300.00 || $330.00 ||
|-
| || add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| Roche LightCycler 480 || $15.00 || per plate || [[BioMicroCenter:Forms#Calenders|CALENDAR]] *available for CORE only || Email biomicro@mit.edu for training
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || || Available for MIT only
|-
| 96-well PLATES || $10.00 || per plate || || Available for MIT only
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR''' ||
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP''' ||
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA ||
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER''' ||
|-
| Varioskan || $18.00 / $20.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''ROBOTICS''' ||
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || ||
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training ||
|-
| colspan="5" | '''NANODROP''' ||
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-15T18:01:24Z

Hilo1: /* SHORT READ LIBRARY - RNA */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90 || Per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00
|-
| NovaSeq S4 50nt || $3,780.00 || $4,158.00 || Per Lane || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq S4 300nt || $5,400.00 || $5,940.00
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00 || Per Lane || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00
|-
| NovaSeqX 10B 300nt Flowcell^^ || $12,160.00 || $13,376.00 || Per Flowcell
|-
| NovaSeqX 25B 300nt Flowcell^^ || $16,200.00 || $17,820.00
|-
| NovaSeqX 25B 100nt Flowcell^^ || $5,750.00 || $6,325.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
! Length !! Reads !! !! !! !!
|-
| 300nt || 5M || $715.00 || $786.50 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,040.00 || $1,144.00
|-
| 100nt || 25M || $925.00 || $1,017.50
|-
| 300nt || 25M || $1,334.00 || $1,467.40
|-
| 600nt || 25M || $1,570.00 || $1,727.00
|-
| '''1000nt''' || 25M || $2,590.00 || $2,849.00
|-
| 100nt || 100M || $1,540.00 || $1,694.00
|-
| 300nt || 100M || $2,125.00 || $2,337.50
|-
| 600nt || 50M || $2,125.00 || $2,337.50
|}

==== MiSeq i100 Walkup ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime !! unit !! Notes
|-
! Length !! Reads !! !! !! !! !!
|-
| Machine usage || || $120.00 || $132.00 || || per flowcell || training and lab storage on BMC servers required.
|-
| 300nt || 5M || $381.00 || $419.10 || 8h || per kit
|-
| 600nt || 5M || $665.00 || $731.50 || 16h ||
|-
| 100nt || 25M || $565.00 || $621.50 || 5h ||
|-
| 300nt || 25M || $920.00 || $1,012.00 || 8h ||
|-
| 600nt || 25M || $1,125.00 || $1,238.00 || 16h ||
|-
| 1000nt || 25M || $1,921.00 || $2,113.10 || 24h ||
|-
| 100nt || 100M || $1,130.00 || $1,243.00 || 5h ||
|-
| 300nt || 100M || $1,638.50 || $1,802.35 || 8h ||
|-
| 600nt || 50M || $1,638.50 || $1,802.35 || 16h ||
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,320.00 || $1,452.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $1,430.00 || $1,573.00
|-
| 300nt V2 kit (eg. 150PE) || $1,595.00 || $1,754.50
|-
| 500nt V2 kit (eg. 250PE) || $1,760.00 || $1,936.00
|-
| 600nt V3 kit (eg. 300PE) || $2,200.00 || $2,420.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $770.00 || $847.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,000.00 || $3,300.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,100.00 || $2,310.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00
|-
| || || 96 || $3,200.00 || $3,520.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Standard Ligation || LSK || $160.00 || $176.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00 ||
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00 ||
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00 ||
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00 ||
|-
| Direct RNA || RNA004 || $160.00 || $176.00 ||
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| DNA || 96 || $700.00 || $770.00 ||
|-
| RNA || 96 || $800.00 || $880.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service''' ||
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION'' ||
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00 ||
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq'' ||
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)'' ||
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION'' ||
|-
| per session || <24h notice || $200.00 || $220.00 ||
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Slide Only || per slide || $150.00 || $165.00 ||
|-
| Optimization Only || per slide || $800.00 || $880.00 ||
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00 ||
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00 ||
|-
| Antibody Add-on || per slide || $360.00 || $396.00 ||
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00 ||
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:''' ||
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00 ||
|-
| FFPE Curl || per curl || $60.00 || $66.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $300.00 || $330.00 ||
|-
| || add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| Roche LightCycler 480 || $15.00 || per plate || [[BioMicroCenter:Forms#Calenders|CALENDAR]] *available for CORE only || Email biomicro@mit.edu for training
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || || Available for MIT only
|-
| 96-well PLATES || $10.00 || per plate || || Available for MIT only
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR''' ||
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP''' ||
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA ||
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER''' ||
|-
| Varioskan || $18.00 / $20.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''ROBOTICS''' ||
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || ||
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training ||
|-
| colspan="5" | '''NANODROP''' ||
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-15T18:00:50Z

Hilo1: /* SHORT READ LIBRARY - DNA */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90 || Per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00
|-
| NovaSeq S4 50nt || $3,780.00 || $4,158.00 || Per Lane || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq S4 300nt || $5,400.00 || $5,940.00
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00 || Per Lane || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00
|-
| NovaSeqX 10B 300nt Flowcell^^ || $12,160.00 || $13,376.00 || Per Flowcell
|-
| NovaSeqX 25B 300nt Flowcell^^ || $16,200.00 || $17,820.00
|-
| NovaSeqX 25B 100nt Flowcell^^ || $5,750.00 || $6,325.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
! Length !! Reads !! !! !! !!
|-
| 300nt || 5M || $715.00 || $786.50 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,040.00 || $1,144.00
|-
| 100nt || 25M || $925.00 || $1,017.50
|-
| 300nt || 25M || $1,334.00 || $1,467.40
|-
| 600nt || 25M || $1,570.00 || $1,727.00
|-
| '''1000nt''' || 25M || $2,590.00 || $2,849.00
|-
| 100nt || 100M || $1,540.00 || $1,694.00
|-
| 300nt || 100M || $2,125.00 || $2,337.50
|-
| 600nt || 50M || $2,125.00 || $2,337.50
|}

==== MiSeq i100 Walkup ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime !! unit !! Notes
|-
! Length !! Reads !! !! !! !! !!
|-
| Machine usage || || $120.00 || $132.00 || || per flowcell || training and lab storage on BMC servers required.
|-
| 300nt || 5M || $381.00 || $419.10 || 8h || per kit
|-
| 600nt || 5M || $665.00 || $731.50 || 16h ||
|-
| 100nt || 25M || $565.00 || $621.50 || 5h ||
|-
| 300nt || 25M || $920.00 || $1,012.00 || 8h ||
|-
| 600nt || 25M || $1,125.00 || $1,238.00 || 16h ||
|-
| 1000nt || 25M || $1,921.00 || $2,113.10 || 24h ||
|-
| 100nt || 100M || $1,130.00 || $1,243.00 || 5h ||
|-
| 300nt || 100M || $1,638.50 || $1,802.35 || 8h ||
|-
| 600nt || 50M || $1,638.50 || $1,802.35 || 16h ||
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,320.00 || $1,452.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $1,430.00 || $1,573.00
|-
| 300nt V2 kit (eg. 150PE) || $1,595.00 || $1,754.50
|-
| 500nt V2 kit (eg. 250PE) || $1,760.00 || $1,936.00
|-
| 600nt V3 kit (eg. 300PE) || $2,200.00 || $2,420.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $770.00 || $847.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. ||
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,000.00 || $3,300.00 ||
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,100.00 || $2,310.00 ||
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00 ||
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00 ||
|-
| || || 96 || $3,200.00 || $3,520.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00 ||
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00 ||
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Standard Ligation || LSK || $160.00 || $176.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00 ||
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00 ||
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00 ||
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00 ||
|-
| Direct RNA || RNA004 || $160.00 || $176.00 ||
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| DNA || 96 || $700.00 || $770.00 ||
|-
| RNA || 96 || $800.00 || $880.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service''' ||
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION'' ||
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00 ||
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq'' ||
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)'' ||
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION'' ||
|-
| per session || <24h notice || $200.00 || $220.00 ||
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Slide Only || per slide || $150.00 || $165.00 ||
|-
| Optimization Only || per slide || $800.00 || $880.00 ||
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00 ||
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00 ||
|-
| Antibody Add-on || per slide || $360.00 || $396.00 ||
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00 ||
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:''' ||
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00 ||
|-
| FFPE Curl || per curl || $60.00 || $66.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $300.00 || $330.00 ||
|-
| || add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| Roche LightCycler 480 || $15.00 || per plate || [[BioMicroCenter:Forms#Calenders|CALENDAR]] *available for CORE only || Email biomicro@mit.edu for training
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || || Available for MIT only
|-
| 96-well PLATES || $10.00 || per plate || || Available for MIT only
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR''' ||
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP''' ||
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA ||
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER''' ||
|-
| Varioskan || $18.00 / $20.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''ROBOTICS''' ||
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || ||
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training ||
|-
| colspan="5" | '''NANODROP''' ||
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-15T18:00:23Z

Hilo1: /* MISEQ SEQUENCING */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90 || Per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00
|-
| NovaSeq S4 50nt || $3,780.00 || $4,158.00 || Per Lane || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq S4 300nt || $5,400.00 || $5,940.00
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00 || Per Lane || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00
|-
| NovaSeqX 10B 300nt Flowcell^^ || $12,160.00 || $13,376.00 || Per Flowcell
|-
| NovaSeqX 25B 300nt Flowcell^^ || $16,200.00 || $17,820.00
|-
| NovaSeqX 25B 100nt Flowcell^^ || $5,750.00 || $6,325.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
! Length !! Reads !! !! !! !!
|-
| 300nt || 5M || $715.00 || $786.50 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,040.00 || $1,144.00
|-
| 100nt || 25M || $925.00 || $1,017.50
|-
| 300nt || 25M || $1,334.00 || $1,467.40
|-
| 600nt || 25M || $1,570.00 || $1,727.00
|-
| '''1000nt''' || 25M || $2,590.00 || $2,849.00
|-
| 100nt || 100M || $1,540.00 || $1,694.00
|-
| 300nt || 100M || $2,125.00 || $2,337.50
|-
| 600nt || 50M || $2,125.00 || $2,337.50
|}

==== MiSeq i100 Walkup ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime !! unit !! Notes
|-
! Length !! Reads !! !! !! !! !!
|-
| Machine usage || || $120.00 || $132.00 || || per flowcell || training and lab storage on BMC servers required.
|-
| 300nt || 5M || $381.00 || $419.10 || 8h || per kit
|-
| 600nt || 5M || $665.00 || $731.50 || 16h ||
|-
| 100nt || 25M || $565.00 || $621.50 || 5h ||
|-
| 300nt || 25M || $920.00 || $1,012.00 || 8h ||
|-
| 600nt || 25M || $1,125.00 || $1,238.00 || 16h ||
|-
| 1000nt || 25M || $1,921.00 || $2,113.10 || 24h ||
|-
| 100nt || 100M || $1,130.00 || $1,243.00 || 5h ||
|-
| 300nt || 100M || $1,638.50 || $1,802.35 || 8h ||
|-
| 600nt || 50M || $1,638.50 || $1,802.35 || 16h ||
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,320.00 || $1,452.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $1,430.00 || $1,573.00
|-
| 300nt V2 kit (eg. 150PE) || $1,595.00 || $1,754.50
|-
| 500nt V2 kit (eg. 250PE) || $1,760.00 || $1,936.00
|-
| 600nt V3 kit (eg. 300PE) || $2,200.00 || $2,420.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $770.00 || $847.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. ||
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00 ||
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00 ||
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00 ||
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00 ||
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00 ||
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00 ||
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. ||
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,000.00 || $3,300.00 ||
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,100.00 || $2,310.00 ||
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00 ||
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00 ||
|-
| || || 96 || $3,200.00 || $3,520.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00 ||
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00 ||
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Standard Ligation || LSK || $160.00 || $176.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00 ||
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00 ||
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00 ||
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00 ||
|-
| Direct RNA || RNA004 || $160.00 || $176.00 ||
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| DNA || 96 || $700.00 || $770.00 ||
|-
| RNA || 96 || $800.00 || $880.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service''' ||
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION'' ||
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00 ||
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq'' ||
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)'' ||
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION'' ||
|-
| per session || <24h notice || $200.00 || $220.00 ||
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Slide Only || per slide || $150.00 || $165.00 ||
|-
| Optimization Only || per slide || $800.00 || $880.00 ||
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00 ||
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00 ||
|-
| Antibody Add-on || per slide || $360.00 || $396.00 ||
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00 ||
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:''' ||
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00 ||
|-
| FFPE Curl || per curl || $60.00 || $66.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $300.00 || $330.00 ||
|-
| || add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| Roche LightCycler 480 || $15.00 || per plate || [[BioMicroCenter:Forms#Calenders|CALENDAR]] *available for CORE only || Email biomicro@mit.edu for training
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || || Available for MIT only
|-
| 96-well PLATES || $10.00 || per plate || || Available for MIT only
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR''' ||
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP''' ||
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA ||
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER''' ||
|-
| Varioskan || $18.00 / $20.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''ROBOTICS''' ||
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || ||
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training ||
|-
| colspan="5" | '''NANODROP''' ||
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-15T17:59:41Z

Hilo1: /* SINGULAR G4 */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90 || Per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00
|-
| NovaSeq S4 50nt || $3,780.00 || $4,158.00 || Per Lane || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq S4 300nt || $5,400.00 || $5,940.00
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00 || Per Lane || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00
|-
| NovaSeqX 10B 300nt Flowcell^^ || $12,160.00 || $13,376.00 || Per Flowcell
|-
| NovaSeqX 25B 300nt Flowcell^^ || $16,200.00 || $17,820.00
|-
| NovaSeqX 25B 100nt Flowcell^^ || $5,750.00 || $6,325.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
! Length !! Reads !! !! !! !!
|-
| 300nt || 5M || $715.00 || $786.50 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,040.00 || $1,144.00 ||
|-
| 100nt || 25M || $925.00 || $1,017.50 ||
|-
| 300nt || 25M || $1,334.00 || $1,467.40 ||
|-
| 600nt || 25M || $1,570.00 || $1,727.00 ||
|-
| '''1000nt''' || 25M || $2,590.00 || $2,849.00 ||
|-
| 100nt || 100M || $1,540.00 || $1,694.00 ||
|-
| 300nt || 100M || $2,125.00 || $2,337.50 ||
|-
| 600nt || 50M || $2,125.00 || $2,337.50 ||
|}

==== MiSeq i100 Walkup ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime !! unit !! Notes
|-
! Length !! Reads !! !! !! !! !!
|-
| Machine usage || || $120.00 || $132.00 || || per flowcell || training and lab storage on BMC servers required.
|-
| 300nt || 5M || $381.00 || $419.10 || 8h || per kit ||
|-
| 600nt || 5M || $665.00 || $731.50 || 16h || ||
|-
| 100nt || 25M || $565.00 || $621.50 || 5h || ||
|-
| 300nt || 25M || $920.00 || $1,012.00 || 8h || ||
|-
| 600nt || 25M || $1,125.00 || $1,238.00 || 16h || ||
|-
| 1000nt || 25M || $1,921.00 || $2,113.10 || 24h || ||
|-
| 100nt || 100M || $1,130.00 || $1,243.00 || 5h || ||
|-
| 300nt || 100M || $1,638.50 || $1,802.35 || 8h || ||
|-
| 600nt || 50M || $1,638.50 || $1,802.35 || 16h || ||
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,320.00 || $1,452.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $1,430.00 || $1,573.00 ||
|-
| 300nt V2 kit (eg. 150PE) || $1,595.00 || $1,754.50 ||
|-
| 500nt V2 kit (eg. 250PE) || $1,760.00 || $1,936.00 ||
|-
| 600nt V3 kit (eg. 300PE) || $2,200.00 || $2,420.00 ||
|-
| 500nt '''NANO''' Run (eg. 250PE) || $770.00 || $847.00 ||
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. ||
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00 ||
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00 ||
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00 ||
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00 ||
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00 ||
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00 ||
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. ||
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,000.00 || $3,300.00 ||
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,100.00 || $2,310.00 ||
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00 ||
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00 ||
|-
| || || 96 || $3,200.00 || $3,520.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00 ||
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00 ||
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Standard Ligation || LSK || $160.00 || $176.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00 ||
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00 ||
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00 ||
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00 ||
|-
| Direct RNA || RNA004 || $160.00 || $176.00 ||
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| DNA || 96 || $700.00 || $770.00 ||
|-
| RNA || 96 || $800.00 || $880.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service''' ||
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION'' ||
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00 ||
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq'' ||
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)'' ||
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION'' ||
|-
| per session || <24h notice || $200.00 || $220.00 ||
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Slide Only || per slide || $150.00 || $165.00 ||
|-
| Optimization Only || per slide || $800.00 || $880.00 ||
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00 ||
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00 ||
|-
| Antibody Add-on || per slide || $360.00 || $396.00 ||
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00 ||
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:''' ||
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00 ||
|-
| FFPE Curl || per curl || $60.00 || $66.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $300.00 || $330.00 ||
|-
| || add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| Roche LightCycler 480 || $15.00 || per plate || [[BioMicroCenter:Forms#Calenders|CALENDAR]] *available for CORE only || Email biomicro@mit.edu for training
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || || Available for MIT only
|-
| 96-well PLATES || $10.00 || per plate || || Available for MIT only
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR''' ||
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP''' ||
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA ||
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER''' ||
|-
| Varioskan || $18.00 / $20.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''ROBOTICS''' ||
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || ||
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training ||
|-
| colspan="5" | '''NANODROP''' ||
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]