BioMicro Center - User contributions [en]

BioMicroCenter:Pricing

2026-04-14T17:50:18Z

Cstjohn:

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| NovaSeq SP 100nt || $5,178.60 || Per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq SP 300nt** || $7,333.20 ||
|-
| NovaSeq SP 500nt || $9,828.00 ||
|-
| NovaSeq S1 100nt** || $8,164.80 ||
|-
| NovaSeq S1 200nt** || $10,432.80 ||
|-
| NovaSeq S1 300nt** || $11,377.80 ||
|-
| NovaSeq S2 100nt** || $14,553.00 ||
|-
| NovaSeq S2 200nt** || $18,144.00 ||
|-
| NovaSeq S2 300nt** || $18,900.00 ||
|-
| NovaSeq S4 50nt || $5,292.00 || Per Lane || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq S4 300nt || $7,560.00 ||
|-
| NovaSeqX 10B 150PE Lane^^ || $2,590.00 || Per Lane || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| NovaSeqX 25B 150PE Lane^^ || $3,640.00 ||
|-
| NovaSeqX 10B 300nt Flowcell^^ || $17,024.00 || Per Flowcell ||
|-
| NovaSeqX 25B 300nt Flowcell^^ || $22,680.00 ||
|-
| NovaSeqX 25B 100nt Flowcell^^ || $8,050.00 ||
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,660.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,400.00 || per lane ||
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,710.00 || per flowcell ||
|-
| || One lane (~400M reads) || $1,925.00 || per lane ||
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,900.00 || per lane ||
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $420.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $2,520.00 || per flowcell ||
|-
| 150 PE: F3 Lane (~100M reads) || $630.00 || per lane ||
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
! Length !! Reads !! !! !!
|-
| 300nt || 5M || $1,001.00 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,456.00 ||
|-
| 100nt || 25M || $1,295.00 ||
|-
| 300nt || 25M || $1,867.60 ||
|-
| 600nt || 25M || $2,198.00 ||
|-
| '''1000nt''' || 25M || $3,626.00 ||
|-
| 100nt || 100M || $2,156.00 ||
|-
| 300nt || 100M || $2,975.00 ||
|-
| 600nt || 50M || $2,975.00 ||
|}

==== MiSeq i100 Walkup ====

Walkup service is not available to Non-MIT users.

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,848.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $2,002.00 ||
|-
| 300nt V2 kit (eg. 150PE) || $2,233.00 ||
|-
| 500nt V2 kit (eg. 250PE) || $2,464.00 ||
|-
| 600nt V3 kit (eg. 300PE) || $3,080.00 ||
|-
| 500nt '''NANO''' Run (eg. 250PE) || $1,078.00 ||
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 72 hour Flowcell || $1,680.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 72 hour Flowcell || pricing in ilabs || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="5" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| LIGATION BASED NEB UltraII || STD || per sample || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="5" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. ||
|-
| HTL NEB UltraII || HTL || 24 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,520.00 ||
|-
| Tagmentation Nextera-XT || STD || per Sample || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $235.00 ||
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,520.00 ||
|-
| 16S Amplicon || HTL || 48 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $910.00 ||
|-
| Other Amplicon || HTL || 48 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $945.00 ||
|-
| colspan="5" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $84.00 ||
|-
| Sample Cleanup || || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="5" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="5" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. ||
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $4,200.00 ||
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,940.00 ||
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $7,000.00 ||
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,800.00 ||
|-
| || || 96 || $4,480.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $7,000.00 ||
|-
| colspan="5" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $84.00 ||
|-
| Sample Cleanup || || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Standard Ligation || LSK || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $70.00 ||
|-
| SMARTseq -> Ligation || Cv4+LSK || $450.00 ||
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $225.00 ||
|-
| Long Insert Prep || RAD (modified, target >100kb) || $393.00 ||
|-
| Direct RNA || RNA004 || $240.00 ||
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| DNA || 96 || $980.00 ||
|-
| RNA || 96 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="4" | '''Assisted Service''' ||
|-
| colspan="4" | ''DIGITAL GENE EXPRESSION'' ||
|-
| 5' RNA / 3' RNA - setup || setup || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $5,460.00 ||
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="4" | ''scATACseq'' ||
|-
| ATACseq - setup || setup || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="4" | ''Multiome (ATAC+3'RNA)'' ||
|-
| MULTI - setup || setup || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="4" | ''CANCELLATION'' ||
|-
| per session || <24h notice || $280.00 ||
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Test Array || slide || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Slide Only || per slide || $210.00 ||
|-
| Optimization Only || per slide || $1,120.00 ||
|-
| Teton Panel || per slide || $10,080.00 ||
|-
| Atlas Short || per slide || $7,700.00 ||
|-
| Antibody Add-on || per slide || $504.00 ||
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $21.00 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $42.00 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $70.00 ||
|-
| qPCR only (Short read quantification test) || per sample || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="4" | '''Sample QC services:''' ||
|-
| Fresh Frozen Curl || per curl || $66.00 ||
|-
| FFPE Curl || per curl || $84.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Dr.Oligo || Assisted || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $420.00 ||
|-
| || add Quench || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to Non-MIT users.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR''' ||
|-
| COVARIS E220 || $28.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP''' ||
|-
| Gel Isolation || $105.00 || per gel (5 lanes) || TBA ||
|-
| Training || $50.00 || per session || TBA || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="5" | '''PLATE READER''' ||
|-
| Varioskan || $27.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="5" | '''ROBOTICS''' ||
|-
| Tecan EVO 150 || $105.00 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| non-filter tips || $11.20 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $16.80 || per box || ||
|-
| Training || $91.00 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]] ||
|-
| colspan="5" | '''NANODROP''' ||
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Informatics Support || per hour || $205.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || N/A || MIT only
|-
| Ingenuity Pathway Analysis || per dataset || $150.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || N/A ||
|-
| Tape Archive / Recovery || per tape || N/A ||
|-
| Informatics Training Classes || per session || N/A ||
|}

MIT:Pricing

2026-04-14T17:26:27Z

Cstjohn: /* OTHER LAB SERVICES */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90 || Per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80 ||
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00 ||
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20 ||
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20 ||
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70 ||
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50 ||
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00 ||
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00 ||
|-
| NovaSeq S4 50nt || $3,780.00 || $4,158.00 || Per Lane || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq S4 300nt || $5,400.00 || $5,940.00 ||
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00 || Per Lane || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00 ||
|-
| NovaSeqX 10B 300nt Flowcell^^ || $12,160.00 || $13,376.00 || Per Flowcell ||
|-
| NovaSeqX 25B 300nt Flowcell^^ || $16,200.00 || $17,820.00 ||
|-
| NovaSeqX 25B 100nt Flowcell^^ || $5,750.00 || $6,325.00 ||
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane ||
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell ||
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane ||
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane ||
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell ||
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane ||
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
! Length !! Reads !! !! !! !!
|-
| 300nt || 5M || $715.00 || $786.50 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,040.00 || $1,144.00 ||
|-
| 100nt || 25M || $925.00 || $1,017.50 ||
|-
| 300nt || 25M || $1,334.00 || $1,467.40 ||
|-
| 600nt || 25M || $1,570.00 || $1,727.00 ||
|-
| '''1000nt''' || 25M || $2,590.00 || $2,849.00 ||
|-
| 100nt || 100M || $1,540.00 || $1,694.00 ||
|-
| 300nt || 100M || $2,125.00 || $2,337.50 ||
|-
| 600nt || 50M || $2,125.00 || $2,337.50 ||
|}

==== MiSeq i100 Walkup ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime !! unit !! Notes
|-
! Length !! Reads !! !! !! !! !!
|-
| Machine usage || || $120.00 || $132.00 || || per flowcell || training and lab storage on BMC servers required.
|-
| 300nt || 5M || $381.00 || $419.10 || 8h || per kit ||
|-
| 600nt || 5M || $665.00 || $731.50 || 16h || ||
|-
| 100nt || 25M || $565.00 || $621.50 || 5h || ||
|-
| 300nt || 25M || $920.00 || $1,012.00 || 8h || ||
|-
| 600nt || 25M || $1,125.00 || $1,238.00 || 16h || ||
|-
| 1000nt || 25M || $1,921.00 || $2,113.10 || 24h || ||
|-
| 100nt || 100M || $1,130.00 || $1,243.00 || 5h || ||
|-
| 300nt || 100M || $1,638.50 || $1,802.35 || 8h || ||
|-
| 600nt || 50M || $1,638.50 || $1,802.35 || 16h || ||
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,320.00 || $1,452.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $1,430.00 || $1,573.00 ||
|-
| 300nt V2 kit (eg. 150PE) || $1,595.00 || $1,754.50 ||
|-
| 500nt V2 kit (eg. 250PE) || $1,760.00 || $1,936.00 ||
|-
| 600nt V3 kit (eg. 300PE) || $2,200.00 || $2,420.00 ||
|-
| 500nt '''NANO''' Run (eg. 250PE) || $770.00 || $847.00 ||
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. ||
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00 ||
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00 ||
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00 ||
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00 ||
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00 ||
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00 ||
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. ||
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,000.00 || $3,300.00 ||
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,100.00 || $2,310.00 ||
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00 ||
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00 ||
|-
| || || 96 || $3,200.00 || $3,520.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00 ||
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00 ||
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Standard Ligation || LSK || $160.00 || $176.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00 ||
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00 ||
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00 ||
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00 ||
|-
| Direct RNA || RNA004 || $160.00 || $176.00 ||
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| DNA || 96 || $700.00 || $770.00 ||
|-
| RNA || 96 || $800.00 || $880.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service''' ||
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION'' ||
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00 ||
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq'' ||
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)'' ||
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION'' ||
|-
| per session || <24h notice || $200.00 || $220.00 ||
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Slide Only || per slide || $150.00 || $165.00 ||
|-
| Optimization Only || per slide || $800.00 || $880.00 ||
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00 ||
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00 ||
|-
| Antibody Add-on || per slide || $360.00 || $396.00 ||
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00 ||
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:''' ||
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00 ||
|-
| FFPE Curl || per curl || $60.00 || $66.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $300.00 || $330.00 ||
|-
| || add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| Roche LightCycler 480 || $15.00 || per plate || [[BioMicroCenter:Forms#Calenders|CALENDAR]] *available for CORE only || Contact [[BioMicroCenter:People|Christopher Hallee]] for training
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || || Available for MIT only
|-
| 96-well PLATES || $10.00 || per plate || || Available for MIT only
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR''' ||
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP''' ||
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA ||
|-
| Training || $25.00 / $27.50 || per session || TBA || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="5" | '''PLATE READER''' ||
|-
| Varioskan || $18.00 / $20.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="5" | '''ROBOTICS''' ||
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || ||
|-
| Training || $65.00 / $71.50 || per hour || Contact [[BioMicroCenter:People|Avanyish Toniappa]] ||
|-
| colspan="5" | '''NANODROP''' ||
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

BioMicroCenter:Pricing

2026-04-14T17:26:06Z

Cstjohn: /* OTHER LAB SERVICES */

{{BioMicroCenter}}

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| NovaSeq SP 100nt || $5,178.60 || Per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq SP 300nt** || $7,333.20 ||
|-
| NovaSeq SP 500nt || $9,828.00 ||
|-
| NovaSeq S1 100nt** || $8,164.80 ||
|-
| NovaSeq S1 200nt** || $10,432.80 ||
|-
| NovaSeq S1 300nt** || $11,377.80 ||
|-
| NovaSeq S2 100nt** || $14,553.00 ||
|-
| NovaSeq S2 200nt** || $18,144.00 ||
|-
| NovaSeq S2 300nt** || $18,900.00 ||
|-
| NovaSeq S4 50nt || $5,292.00 || Per Lane || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq S4 300nt || $7,560.00 ||
|-
| NovaSeqX 10B 150PE Lane^^ || $2,590.00 || Per Lane || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| NovaSeqX 25B 150PE Lane^^ || $3,640.00 ||
|-
| NovaSeqX 10B 300nt Flowcell^^ || $17,024.00 || Per Flowcell ||
|-
| NovaSeqX 25B 300nt Flowcell^^ || $22,680.00 ||
|-
| NovaSeqX 25B 100nt Flowcell^^ || $8,050.00 ||
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,660.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,400.00 || per lane ||
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,710.00 || per flowcell ||
|-
| || One lane (~400M reads) || $1,925.00 || per lane ||
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,900.00 || per lane ||
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $420.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $2,520.00 || per flowcell ||
|-
| 150 PE: F3 Lane (~100M reads) || $630.00 || per lane ||
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
! Length !! Reads !! !! !!
|-
| 300nt || 5M || $1,001.00 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,456.00 ||
|-
| 100nt || 25M || $1,295.00 ||
|-
| 300nt || 25M || $1,867.60 ||
|-
| 600nt || 25M || $2,198.00 ||
|-
| '''1000nt''' || 25M || $3,626.00 ||
|-
| 100nt || 100M || $2,156.00 ||
|-
| 300nt || 100M || $2,975.00 ||
|-
| 600nt || 50M || $2,975.00 ||
|}

==== MiSeq i100 Walkup ====

Walkup service is not available to Non-MIT users.

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,848.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $2,002.00 ||
|-
| 300nt V2 kit (eg. 150PE) || $2,233.00 ||
|-
| 500nt V2 kit (eg. 250PE) || $2,464.00 ||
|-
| 600nt V3 kit (eg. 300PE) || $3,080.00 ||
|-
| 500nt '''NANO''' Run (eg. 250PE) || $1,078.00 ||
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 72 hour Flowcell || $1,680.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 72 hour Flowcell || pricing in ilabs || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="5" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| LIGATION BASED NEB UltraII || STD || per sample || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="5" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. ||
|-
| HTL NEB UltraII || HTL || 24 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,520.00 ||
|-
| Tagmentation Nextera-XT || STD || per Sample || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $235.00 ||
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,520.00 ||
|-
| 16S Amplicon || HTL || 48 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $910.00 ||
|-
| Other Amplicon || HTL || 48 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $945.00 ||
|-
| colspan="5" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $84.00 ||
|-
| Sample Cleanup || || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="5" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="5" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. ||
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $4,200.00 ||
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,940.00 ||
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $7,000.00 ||
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,800.00 ||
|-
| || || 96 || $4,480.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $7,000.00 ||
|-
| colspan="5" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $84.00 ||
|-
| Sample Cleanup || || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Standard Ligation || LSK || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $70.00 ||
|-
| SMARTseq -> Ligation || Cv4+LSK || $450.00 ||
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $225.00 ||
|-
| Long Insert Prep || RAD (modified, target >100kb) || $393.00 ||
|-
| Direct RNA || RNA004 || $240.00 ||
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| DNA || 96 || $980.00 ||
|-
| RNA || 96 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="4" | '''Assisted Service''' ||
|-
| colspan="4" | ''DIGITAL GENE EXPRESSION'' ||
|-
| 5' RNA / 3' RNA - setup || setup || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $5,460.00 ||
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="4" | ''scATACseq'' ||
|-
| ATACseq - setup || setup || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="4" | ''Multiome (ATAC+3'RNA)'' ||
|-
| MULTI - setup || setup || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="4" | ''CANCELLATION'' ||
|-
| per session || <24h notice || $280.00 ||
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Test Array || slide || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Slide Only || per slide || $210.00 ||
|-
| Optimization Only || per slide || $1,120.00 ||
|-
| Teton Panel || per slide || $10,080.00 ||
|-
| Atlas Short || per slide || $7,700.00 ||
|-
| Antibody Add-on || per slide || $504.00 ||
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $21.00 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $42.00 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $70.00 ||
|-
| qPCR only (Short read quantification test) || per sample || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="4" | '''Sample QC services:''' ||
|-
| Fresh Frozen Curl || per curl || $66.00 ||
|-
| FFPE Curl || per curl || $84.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Dr.Oligo || Assisted || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $420.00 ||
|-
| || add Quench || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to Non-MIT users.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR''' ||
|-
| COVARIS E220 || $28.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP''' ||
|-
| Gel Isolation || $105.00 || per gel (5 lanes) || TBA ||
|-
| Training || $50.00 || per session || TBA || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="5" | '''PLATE READER''' ||
|-
| Varioskan || $27.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="5" | '''ROBOTICS''' ||
|-
| Tecan EVO 150 || $105.00 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| non-filter tips || $11.20 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $16.80 || per box || ||
|-
| Training || $91.00 || per hour || Contact [[BioMicroCenter:People|Avanyish Toniappa]] ||
|-
| colspan="5" | '''NANODROP''' ||
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Informatics Support || per hour || $205.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || N/A || MIT only
|-
| Ingenuity Pathway Analysis || per dataset || $150.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || N/A ||
|-
| Tape Archive / Recovery || per tape || N/A ||
|-
| Informatics Training Classes || per session || N/A ||
|}

== SEQUENCING - MID OUTPUT ==
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason. 
=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===
{| class="wikitable" border=1
! ELEMENT AVITI SEQUENCING
!width=80| Lane/Flowcell
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=80| unit
! Notes
|-
|rowspan=2| 75 PE
| Full flowcell (2 lane, 800m read)
|align="right"| $1,900
|align="right"| $2,090
|align="right"| $2,660
|align="center"| per flowcell
|rowspan=5| Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
|One lane (~400m read)
|align="right"| $1,000
|align="right"| $1,100
|align="right"| $1,400
|align="center"| per lane
|-
|rowspan=2| 150 PE
| Full flowcell (2 lane, ~800m read)
|align="right"| $2,650
|align="right"| $2,915
|align="right"| $3,710
|align="center"| per flowcell
|-
|One lane (~400m read)
|align="right"| $1,375
|align="right"| $1,513
|align="right"| $1,925
|align="center"| per lane
|-
| 300 PE
| Full flowcell (~200m reads)
|align="right"| $3,500
|align="right"| $3,850
|align="right"| $4,900
|align="center"| per lane
|}


== SEQUENCING - LOW OUTPUT ==
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason. 
=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===
{| class="wikitable" border=1
! SINGULAR G4 SEQUENCING
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=80| unit
! Notes
|-
|50 PE: F3 Lane (~100m reads)
|align="right"| $300
|align="right"| $330
|align="right"| $420
|align="center" | per lane
|rowspan=3| Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
|Rapid 100nt: F3 flowcell (~400m reads)*
|align="right"| $1200
|align="right"| $1321
|align="right"| $1680
|align="center"| per flowcell
|-
|150 PE: F3 Lane (~100m reads)
|align="right"| $450
|align="right"| $495
|align="right"| $630
|align="center"| per lane

|}
* Rapid flowcells are required for adapted Illumina libraries.

=== MISEQ SEQUENCING ===
==== MiSeq i100 Assisted ====
{| class="wikitable" border=1
!colspan="2"| MISEQ i100 SEQUENCING
!rowspan=2 width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!rowspan=2 width=80| MIT
!rowspan=2 width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!rowspan=2 width=80| unit
!rowspan=2| Notes
|-
!Length
!Reads
|-
|300nt
|5M
|align="right"| $715
|align="right"| $786.5
|align="right"| $1001
|align="center" rowspan=9| per flowcell
|rowspan=9| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
|600nt
|5M
|align="right"| $1040
|align="right"| $1144
|align="right"| $1456
|-
|100nt
|25M
|align="right"| $925
|align="right"| $1017.5
|align="right"| $1295
|-
|300nt
|25M
|align="right"| $1334
|align="right"| $1467.4
|align="right"| $1867.6
|-
|600nt
|25M
|align="right"| $1570
|align="right"| $1727
|align="right"| $2198
|-
|1000nt
|25M
|align="right"| $2590
|align="right"| $2849
|align="right"| $3626
|-
|100nt
|100M
|align="right"| $1540
|align="right"| $1694
|align="right"| $2156
|-
|300nt
|100M
|align="right"| $2125
|align="right"| $2337.5
|align="right"| $2975
|-
|600nt
|50M
|align="right"| $2125
|align="right"| $2337.5
|align="right"| $2975
|}

==== MiSeq i100 Walkup ====
{| class="wikitable" border=1
!colspan="2"| MISEQ i100 SEQUENCING
!rowspan=2 width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!rowspan=2 width=80| MIT
!rowspan=2 width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!rowspan=2 width=80| unit
!rowspan=2 width=80| RunTime
!rowspan=2| Notes
|-
!Length
!Reads
|-
|colspan=2 | Machine usage
|align="right"| $120
|align="right"| $132
|align="right"| NA
|
|align="center"| per flowcell
| rowspan=10| training and lab storage on BMC servers required.
|-
|300nt
|5M
|align="right"| $381
|align="right"| $419.1
|align="right"| NA
|align="center"| 8h
|align="center" rowspan=9| per kit
|-
|600nt
|5M
|align="right"| $665
|align="right"| $731.5
|align="right"| NA
|align="center"| 16h
|-
|100nt
|25M
|align="right"| $565
|align="right"| $621.5
|align="right"| NA
|align="center"| 5h
|-
|300nt
|25M
|align="right"| $920
|align="right"| $1012
|align="right"| NA
|align="center"| 8h
|-
|600nt
|25M
|align="right"| $1125
|align="right"| $1238
|align="right"| NA
|align="center"| 16h
|-
|1000nt
|25M
|align="right"| $1921
|align="right"| $2113.1
|align="right"| NA
|align="center"| 24h
|-
|100nt
|100M
|align="right"| $1130
|align="right"| $1243
|align="right"| NA
|align="center"| 5h
|-
|300nt
|100M
|align="right"| $1638.5
|align="right"| $1802.35
|align="right"| NA
|align="center"| 8h
|-
|600nt
|50M
|align="right"| $1638.5
|align="right"| $1802.35
|align="right"| NA
|align="center"| 16h
|}

==== MiSeq classic ====
* MiSeq is off contract and will be retired upon instrument failure
{| class="wikitable" border=1
! MISEQ SEQUENCING
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=80| unit
! Notes
|-
|70nt V2 kit (eg. 30PE or 70SE)
|align="right"| $1,320
|align="right"| $1,452
|align="right"| $1,848
|align="center" rowspan=6| per lane
|rowspan=6| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
|150nt V3 kits (eg. 75PE)
|align="right"| $1,430
|align="right"| $1,573
|align="right"| $2,002
|-
|300nt V2 kit (eg. 150PE)
|align="right"| $1,595
|align="right"| $1,754.50
|align="right"| $2,233
|-
|500nt V2 kit (eg. 250PE)
|align="right"| $1,760
|align="right"| $1,936
|align="right"| $2,464
|-
|600nt V3 kit (eg. 300PE)
|align="right"| $2,200
|align="right"| $2,420
|align="right"| $3,080
|-
|500nt '''NANO''' Run (eg. 250PE)
|align="right"| $770
|align="right"| $847
|align="right"| $1,078
|}

=== OTHER SHORT READ SERVICES ===
{| class="wikitable" border=1
! SHORT READ SUPPORTING SERVICES
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=80| unit
! Notes
|-
| Quality Control Only -only one QC is included per sequencing lane.
|align="right"| $35
|align="right"| $38.50
|align="right"| $49
|align="center"| per sample
| Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling.
|align="right"| $15
|align="right"| $16.50
|align="right"| $21
|align="center"| per sample
| Includes Fluorometric quantification and Frag.Analyzer
|-
|qPCR only (short read)
|align="right"| $25
|align="right"| $27.50
|align="right"| $35
|align="center"| per sample
| Includes qPCR values from standard QC only
|-
|qPCR only - full plate(short read)
|align="right"| $400
|align="right"| $440
|align="right"| $560
|align="center"| per sample
| excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable" border=1
! PROMETHION SEQUENCING
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=80| unit
! Notes
|-
| 72 hour Flowcell
|align="right"| $1,200
|align="right"| $1,320
|align="right"| $1,680
|align="center"|per Flowcell
||Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable" border=1
! PACBIO REVIO SEQUENCING^^
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=80| unit
! Notes
|-
| 72 hour Flowcell
|colspan=3 | pricing in ilabs
|align="center"|per Flowcell
||Fastq stored for 1y. Trace data stored only 30d.
|}

^^ - through collaboration with nearby academic shared resources

== LIBRARY GENERATION ==
=== SHORT READ LIBRARY - CONVERSION ===
{| class="wikitable" border=1
!width=100| Input library
!width=100| Convert To
!width=100| Method
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|align="center"|Illumina
|align="center"|Singular
|align="center"|Index Overwrite (per sample)
|align="right"| $30
|align="right"| $33
|align="right"| $42
| Includes QC and reindexing
|-
|align="center"|Illumina
|align="center"|Singular
|align="center"|P5/P7 conversion (per pool)
|align="right"| $100
|align="right"| $110
|align="right"| $140
| Includes QC. Samples not reindexed Requires full flowcell for sequencing (Rapid) 
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable" border=1
!width=160| ILLUMINA Sample Preparation
!width=60| Type
!width=60| Batch Size
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
! colspan="4" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
! colspan="3" style="color: blue;" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
|rowspan=3 |LIGATION BASED NEB UltraII
|align="center"|STD
|align="center"| per sample
|align="right"| $105
|align="right"| $115
|align="right"| $157
| Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|- style="color: blue;"
|align="center" rowspan=2|HTL
|align="center"| 24
|align="right"| $900
|align="right"| $990
|align="right"| $1,260
|rowspan=2 | Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|- style="color: blue;"
|align="center"| 96
|align="right"| $1,800
|align="right"| $1,980
|align="right"| $2,520
|-

|Tagmentation NEXTERA-XT
|align="center" rowspan=2|STD
|align="center" rowspan=2|per Sample
|align="right"| $105
|align="right"| $115
|align="right"| $157
| rowspan="2" | Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
|Tagmentation NEXTERA-FLEX
|align="right"| $157
|align="right"| $173
|align="right"| $236

|- style="color: blue;"
|rowspan=2 | NEXTERA-XT OR FLEX
|rowspan=2 align="center"| HTL
|align="center"| ''24''
|align="right"| $900
|align="right"| $990
|align="right"| $1,260
|rowspan=2 | Includes tagmentation, indexing, enrichment, and quality control spotcheck(SYBR and FA).
|-
|align="center" style="color: blue;" | ''96''
|align="right" style="color: blue;" | $1,800
|align="right" style="color: blue;" | $1,980
|align="right" style="color: blue;" | $2,520

|-style="color: blue;"
|rowspan="2" | 16S Amplicon
|align="center" rowspan=2 | HTL
|align="center"|48
|align="right" | $1,900
|align="right" | $2,090
|align="right" | $2,660
|rowspan="2" | Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-style="color: blue;"
|align="center"|add'l 48
|align="right" | $650
|align="right" | $715
|align="right" | $910

|-style="color: blue;"
|rowspan="2" | Other Amplicon
|rowspan=2 align="center"| HTL
|align="center" |48
|align="right" | $1,600
|align="right" | $1,760
|align="right" | $2,240
|rowspan="2" | Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|- style="color: blue;"
|align="center"|add'l 48
|align="right" | $630
|align="right" | $693
|align="right" | $945
|-
! colspan="7" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
|style="color: blue;" colspan=2| High Throughput Setup
|align="center" style="color: blue;" |48 samples
|align="right" style="color: blue;" | $60
|align="right" style="color: blue;" | $66
|align="right" style="color: blue;" | $84
|
|-
|colspan=2|Sample Cleanup
|align="center"|per sample
|align="right"| $5
|align="right"| $5.50
|align="right"| $7
|SPRI cleanup of samples to remove impurities.
|-
|style="color: blue;" colspan=2|Quantification
|align="center" style="color: blue;" |per sample
|align="right" style="color: blue;" | $10
|align="right" style="color: blue;" | $11
|align="right" style="color: blue;" | $14
|Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable" border=1
!width=160| ILLUMINA Sample Preparation
!width=60| Type
!width=60| Batch Size
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
! colspan="4" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
! colspan="3" style="color: blue;" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
|rowspan="3"|polyA based isolation NEB UltraII RNAseq
|align="center"|STD
|align="center"| per sample
|align="right"| $157
|align="right"| $173
|align="right"| $236
|Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
|rowspan=2 align="center" style="color: blue;"| HTL (NEB only)
|align="center" style="color: blue;" | ''24''
|align="right" style="color: blue;" | $1,500
|align="right" style="color: blue;" | $1,650
|align="right" style="color: blue;" | $2,100
|Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
|align="center" style="color: blue;" | ''96''
|align="right" style="color: blue;" | $3,000
|align="right" style="color: blue;" | $3,300
|align="right" style="color: blue;" | $4,200
|Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-

|rowspan="2"| High Throughput 3' Digital Gene Expression (HT3DGE)
|rowspan=2 align="center" style="color: blue;"| HTL
|align="center" style="color: blue;"| ''24''
|align="right" style="color: blue;" | $1,050
|align="right" style="color: blue;" | $1,155
|align="right" style="color: blue;" | $1,470
| rowspan="2" style="color: blue;" | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
|align="center" style="color: blue;" | ''96''
|align="right" style="color: blue;" | $2,100
|align="right" style="color: blue;" | $2,310
|align="right" style="color: blue;" | $2,940
|-

|rowspan="3"|Total RNA sequencing NEB RNAseq +Ribodepletion
|align="center"|STD
|align="center"| per sample
|align="right"| $235
|align="right"| $258.5
|align="right"| $352.5
|Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
|rowspan=2 align="center" style="color: blue;"| HTL (NEB only, covaris RNA fragmentation)
|align="center" style="color: blue;" | ''24''
|align="right" style="color: blue;" | $2,500
|align="right" style="color: blue;" | $2,750
|align="right" style="color: blue;" | $3,500
|rowspan=2|Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
|align="center" style="color: blue;" | ''96''
|align="right" style="color: blue;" | $5,000
|align="right" style="color: blue;" | $5,500
|align="right" style="color: blue;" | $7,000
|-

|rowspan=3|Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex
|align="center"| '''STD''' v4
|align="center"|per sample
|align="right"| $262
|align="right"| $289
|align="right"| $394
| rowspan="3" | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
|rowspan="2" align="center" style="color: blue;"| '''HTL''' v2
|align="center" style="color: blue;" | ''24''
|align="right" style="color: blue;" | $2,000
|align="right" style="color: blue;" | $2,200
|align="right" style="color: blue;" | $2,800
|-
|align="center" style="color: blue;" | ''96''
|align="right" style="color: blue;" | $3,200
|align="right" style="color: blue;" | $3,520
|align="right" style="color: blue;" | $4,480
|-

|rowspan="3"|Low Input Mammalian Total Clontech ZapR Kit
|align="center"| STD
|align="center"| per sample
|align="right"| $210
|align="right"| $231
|align="right"| $315
| rowspan="1" | Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
|rowspan=2 align="center" style="color: blue;"| HTL (covaris RNA fragmentation)
|align="center" style="color: blue;" | ''24''
|align="right" style="color: blue;" | $3000
|align="right" style="color: blue;" | $3300
|align="right" style="color: blue;" | $4200
|rowspan=2|Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
|align="center" style="color: blue;" | ''96''
|align="right" style="color: blue;" | $5000
|align="right" style="color: blue;" | $5500
|align="right" style="color: blue;" | $7000
|-
| Small RNA
|align="center"| STD
| align="center"|per sample
| align="right"|$289
| align="right"|$318
| align="right"|$433
| Uses NEB, BIOO or QIAGEN small RNA kit
|-
! colspan="7" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
|colspan=2 style="color: blue;" | High Throughput Setup
|align="center" style="color: blue;" |48 samples
|align="right" style="color: blue;" | $60
|align="right" style="color: blue;" | $66
|align="right" style="color: blue;" | $84
|
|-
|colspan=2|Sample Cleanup
|align="center"|per sample
|align="right"| $5
|align="right"| $5.50
|align="right"| $7
|SPRI cleanup of samples to remove impurities.
|-
|colspan=2 style="color: blue;" |Quantification
|align="center" style="color: blue;" |per sample
|align="right" style="color: blue;" | $10
|align="right" style="color: blue;" | $11
|align="right" style="color: blue;" | $14
|Quantification of samples (FA) prior to arraying.
|-
|}

.

=== LONG READ LIBRARIES ===
{| class="wikitable" border=1
!Oxford Nanopore Sample Preparation
!width=120| Nanopore Kit
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|Standard Ligation || LSK || $160 || $176 || $240
|rowspan=6|Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
|Native Barcoding (ligation indexing) || - || $50 || $55 || $70
|-
|SMARTseq -> Ligation || Cv4+LSK || $300 || $330 || $420
|-
|Rapid Prep (tagment - ~10-20kb) || RAD || $150 || $165 || $225
|-
|Long Insert Prep || RAD (modified, target >100kb) || $262 || $289 || $393
|-
|Direct RNA || RNA004 || $160 || $176 || $240
|}

== SAMPLE EXTRACTION ==
=== CHEMAGIC360 ===
{| class="wikitable" border=1
!TYPE
!width=120| Batch Size
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|DNA
|96
|$700
|$770
|$980
|
|-
|RNA
|96
|$800
|$880
|$1120
|
|}

== SINGLE CELL & SPATIAL SERVICES ==
=== 10X CHROMIUM ===
{| class="wikitable" border=1
! 10X USAGE
!width=120| Batch Size
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|colspan=6 align="center"|'''Assisted Service'''
|-
|colspan=6 |''DIGITAL GENE EXPRESSION''
|-
|rowspan=2 |5' RNA 3' RNA
|setup || $1,015 || $1,116.50 || $1,421 || charge per submission
|-
|per sample|| $1,750 || $1,925 || $2,450 || Includes QC of cDNA and creation of Short Read Library
|-
|rowspan=2 |5' RNA 3' RNA - on chip multiplexing
|setup || $1,150 || $1,265 || $1,1610 || charge per submission
|-
|per 4 samples|| $2,305 || $2,535 || $3,227 || Includes QC of cDNA and creation of Short Read Library
|-
|rowspan=2 |Fixed RNA (Flex v2) probe based. human/mouse only
|setup || $2700 || $2970 || $3780 || charge per submission - upto 48 samples
|-
|per sample || $270|| $297 || $378 || Includes QC of cDNA and creation of Short Read Library
|-
|rowspan=2 |Amplicon (CITE-SEQ, TCR, BCR, antibody barcocde, etc // per amplicon)
|setup || $470 || $517 || $658 || additional prep beyond DGE
|-
|per sample|| $45 || $50 || $63 || Includes QC of cDNA and creation of Short Read Library
|-
|colspan=6 |''scATACseq''
|-
|rowspan=2 |ATACseq
|setup || $880 || $968 || $1,232 || charge per submission
|-
|per sample|| $1,675|| $1,843|| $2,345|| Includes QC and creation of Short Read Library
|-
|colspan=6 |''Multiome (ATAC+3'RNA)''
|-
|rowspan=2 |MULTI
|setup || $1,800|| $1,980|| $2,520|| charge per submission
|-
|per sample|| $3,100|| $3,410|| $4,340|| Includes QC of cDNA and creation of Short Read Library
|-
|colspan=6 |''CANCELLATION''
|-
|per session || || $200|| $220|| $280|| <24h notice
|}
*please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===
{| class="wikitable" border=1
! SLIDES
!width=120| Batch Size
!width=80| KI Only
!width=80| [[BioMicroCenter:CoreDeps|Other CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|Test Array ||slide|| $1,300|| $1,511|| $1,607|| $2435|| recommended 1x per tissue type for fresh frozen
|-
|Sample Slide - Fresh Frozen ||1 slide / 4 tiles|| $6,210|| $6,340|| $6,940|| $9,067 || Includes H&E stain images + Library Generation
|-
|Sample Slide - FFPE (hs/mm only) ||1 slide / 2 tiles|| $4,550|| $4,550|| $4,996|| $6,359 || Includes library generation only.
|-
|Sample Slide - HD (hs/mm only) ||1 slide / 2 tiles|| $7,850|| $7,850|| $8,596|| $10,940 || Includes library generation only.
|}

=== AVITI24 ===
<div style="background:#fff8e6; border-left:4px solid #c8960c; padding:8px 12px; margin-bottom:8px;">'''Beta Service — Early Access:''' Consultation with BMC staff is required before submitting samples. See [[BioMicroCenter:SpTx|Spatial Transcriptomics]] for full service details.</div>
{| class="wikitable" border=1
! AVITI24 CYTOPROFILING / DISS
!width=80|UNIT
!width=80|[[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80|MIT
!width=80|[[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|Atlas Panel (Teton CytoProfiling)
|rowspan=5 align=center| per slide 1-, 12-, 48-well
|align=right| $7,200
|align=right| $7,920
|align=right| $10,080
|RNA + protein + morphology
|-
|Atlas Low (DISS — targeted in situ sequencing)
|align=right| $5,500
|align=right| $6,050
|align=right| $7,700
|User-designed probe; can run simultaneously with Atlas Panel
|-
|Antibody Add-on
|align=right| $360
|align=right| $396
|align=right| $504
|Custom antibody targets; consult BMC staff
|-
|Optimization Run
|align=right| $800
|align=right| $880
|align=right| $1,120
|Charged only if optimization slide fails
|-
|Slide Only (consumable)
|align=right| $150
|align=right| $165
|align=right| $210
|
|}
* High-output DISS (whole transcriptome) not yet released — contact BMC staff.

== OTHER GENOMICS SERVICES ==

 

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===
{| class="wikitable" border=1
! QUALITY CONTROL
!width=100| unit
!width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=100| MIT
!width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|Fragment Analyzer: RNA or DNA
|per sample ||$15 || $16.50 || $21
|
|-
|Fragment Analyzer: Preloaded row
| per row (12) ||$80||$88 || $112
| 2ul per sample in each well using plates from BMC
|-
|FemtoPulse: RNA or DNA
|per sample || $30 || $33 || $42
|
|-
|FemtoPulse: Preloaded Row
|per row (12) || $160 || $176 || $224
| 2ul per sample in each well using plates from BMC
|-
|BioAnalyzer: Walkup Service
|per chip|| $50 || $55 || $70
|
|-
|qPCR only (Short read quantification test)
|per sample || $25 || $27.50 || $35
| 4 point serial dilution
|-
|Full plate qPCR (short read quantification test)
|per plate || $400 || $440 || $560
| full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
|colspan=6 align="center"| Sample QC services:
|-
|Fresh Frozen Curl
|rowspan=2|per curl
| $40|| $44 || $56
|
|-
|FFPE Curl
| $60 || $66 || $84
|
|-
|}

=== OLIGO SYNTHESIS ===
{| class="wikitable" border=1
!Platform
!width=100| Service
!width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=100| MIT
!width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|rowspan=3| Dr.Oligo
|Assisted
|$2000 +consumables
|$2200 +consumables
|$2800 +consumables
|includes setup and run of the instrument only. Large reagents / N2 included.
|-
|Walkup
|$1000 +consumables
|$1100 +consumables
|$1400 +consumables
|includes run of the instrument only. Large reagents / N2 included.
|-
|Training
|$4000
|$4400
|$5600
|ncludes two instrument runs.
|-
|rowspan=6| DNAscript Syntax
|Setup (per run)
|$1000
|$1100
|$1400
|Per run cost
|-
|2000 nucleotide synthesis
|$500
|$550
|$700
|Up to 96 samples
|-
|8000 nucleotide synthesis
|$1500
|$1650
|$2100
|Up to 96 samples
|-
|add Biotin (max48)
|$115
|$126
|$161
|rowspan = 3| max 48 per run and 2 per oligo. Couple to T only.
|-
|add Fluor
|$300
|$330
|$420
|-
|add quench
|$100
|$110
|$140
|}

== [[BioMicroCenter:RTPCR|REAL TIME PCR]] ==

{| border=1
! OTHER LAB SERVICES
!width=100| [[BioMicroCenter:CoreDeps|CORE LAB / MIT]]
!width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=100| unit
! SIGN UP
! Notes
|-
| Roche LightCycler 480
|align="right"| $15
|align="right"| N/A
|align="center"| per plate
| [[BioMicroCenter:Forms#Calenders|CALENDAR]] *available for CORE only
| Contact [[BioMicroCenter:People|Christopher Hallee]] for training
|-
| SYBR Green (KAPA/Roche)
|align="right"| $45
|align="right"| N/A
|align="center"| per ml
|
| Available for MIT only
|-
| 96-well PLATES
|align="right"| $10
|align="right"| N/A
|align="center"| per plate
|
| Available for MIT only
|-
|}
.
 

== OTHER LAB SERVICES ==
{| border=1
! OTHER LAB SERVICES
!width=100| [[BioMicroCenter:CoreDeps|CORE LAB / MIT]]
!width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=100| unit
! SIGN UP
! Notes
|-
|colspan=6 align="center"| '''COVARIS SONICATOR'''
|-
|COVARIS E220
|align="right"|$20
|align="right"|$30
|per hour
|[https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar]
|tubes available for purchase.
|-

| colspan="6" align="center"| '''PIPPIN PREP'''
|-
|Gel Isolation
|align="right"| $75
|align="right"| $105
|align="center"| per gel (5 lanes)
| TBA
|
|-
|Training
|align="right"| $25
|align="right"| $50
|align="center"| per session
| TBA
| Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="6" align="center"| '''PLATE READER'''
|-
| Varioskan
|align="right"| $18
|align="right"| $27
|align="center"| per hour
| [[BioMicroCenter:Forms#Calenders|CALENDAR]]
| Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="6" align="center"| '''ROBOTICS'''
|-
| Tecan EVO 150
|align="right"| $75
|align="right"| $105
|align="center"| per 1h block - based on scheduled time.
| [[BioMicroCenter:Forms#Calenders|CALENDAR]]
|
|-
|non-filter tips
|align="right"| $8
|align="right"| $11.20
|align="center"| per box
|
|** Please let us know if there is additional plasticware you would like us to stock.
|-
|Filter tips
|align="right"| $12.00
|align="right"| $16.80
|align="center"| per box
|
|
|-
| Training
|align="right"| $65
|align="right"| $91
|align="center"| per hour
| Contact [[BioMicroCenter:People|Tatum Murdock]]
|
|-
| colspan="6" align="center"| '''NANODROP'''
|-
| Nanodrop
|align="right"| --
|align="right"| --
|align="center"|
| Walk up service
| * no charge
|-
|}
 

== COMPUTATIONAL SERVICES ==
{| class="wikitable" border=1
! COMPUTATIONAL SERVICES
!width=200| unit
!width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=100| Other MIT / non-MIT
! Notes
|-
| Informatics Support
| align="right"| per hour
| align="right"| $90
| align="right"| $125 / $205
| Very limited support for non-MIT users
|-
| Informatics Share
| align="right"| per 0.1FTE
| align="right"| $1,250
| align="right"| N/A
| MIT only. Priority access to informatics.
|-
|Ingenuity Pathway Analysis
|align="right"|per dataset
|align="right" | $100
|align="right" | $110/$150
| Recovery of licence cost.
|-
|Active Data storage
|align="right"| per TB per year
|align="right"| $100
|align="right"| $300 / N/A
|
|-
| Tape Archive / Recovery
|align="right"| per tape
|align="right"| $60
|align="right"| $70
|
|-
| Informatics Training Classes
|align="right"| per session
|align="right"| $50
|align="right"| $100
|
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

MIT:Pricing

2026-04-14T17:24:40Z

Cstjohn: MIT Pricing Page

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90 || Per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80 ||
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00 ||
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20 ||
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20 ||
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70 ||
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50 ||
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00 ||
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00 ||
|-
| NovaSeq S4 50nt || $3,780.00 || $4,158.00 || Per Lane || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq S4 300nt || $5,400.00 || $5,940.00 ||
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00 || Per Lane || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00 ||
|-
| NovaSeqX 10B 300nt Flowcell^^ || $12,160.00 || $13,376.00 || Per Flowcell ||
|-
| NovaSeqX 25B 300nt Flowcell^^ || $16,200.00 || $17,820.00 ||
|-
| NovaSeqX 25B 100nt Flowcell^^ || $5,750.00 || $6,325.00 ||
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane ||
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell ||
|-
| || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane ||
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane ||
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell ||
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane ||
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
! Length !! Reads !! !! !! !!
|-
| 300nt || 5M || $715.00 || $786.50 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,040.00 || $1,144.00 ||
|-
| 100nt || 25M || $925.00 || $1,017.50 ||
|-
| 300nt || 25M || $1,334.00 || $1,467.40 ||
|-
| 600nt || 25M || $1,570.00 || $1,727.00 ||
|-
| '''1000nt''' || 25M || $2,590.00 || $2,849.00 ||
|-
| 100nt || 100M || $1,540.00 || $1,694.00 ||
|-
| 300nt || 100M || $2,125.00 || $2,337.50 ||
|-
| 600nt || 50M || $2,125.00 || $2,337.50 ||
|}

==== MiSeq i100 Walkup ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime !! unit !! Notes
|-
! Length !! Reads !! !! !! !! !!
|-
| Machine usage || || $120.00 || $132.00 || || per flowcell || training and lab storage on BMC servers required.
|-
| 300nt || 5M || $381.00 || $419.10 || 8h || per kit ||
|-
| 600nt || 5M || $665.00 || $731.50 || 16h || ||
|-
| 100nt || 25M || $565.00 || $621.50 || 5h || ||
|-
| 300nt || 25M || $920.00 || $1,012.00 || 8h || ||
|-
| 600nt || 25M || $1,125.00 || $1,238.00 || 16h || ||
|-
| 1000nt || 25M || $1,921.00 || $2,113.10 || 24h || ||
|-
| 100nt || 100M || $1,130.00 || $1,243.00 || 5h || ||
|-
| 300nt || 100M || $1,638.50 || $1,802.35 || 8h || ||
|-
| 600nt || 50M || $1,638.50 || $1,802.35 || 16h || ||
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,320.00 || $1,452.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $1,430.00 || $1,573.00 ||
|-
| 300nt V2 kit (eg. 150PE) || $1,595.00 || $1,754.50 ||
|-
| 500nt V2 kit (eg. 250PE) || $1,760.00 || $1,936.00 ||
|-
| 600nt V3 kit (eg. 300PE) || $2,200.00 || $2,420.00 ||
|-
| 500nt '''NANO''' Run (eg. 250PE) || $770.00 || $847.00 ||
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. ||
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00 ||
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00 ||
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $1,800.00 || $1,980.00 ||
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $650.00 || $715.00 ||
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $630.00 || $693.00 ||
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00 ||
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. ||
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,000.00 || $3,300.00 ||
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,100.00 || $2,310.00 ||
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00 ||
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00 ||
|-
| || || 96 || $3,200.00 || $3,520.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $5,000.00 || $5,500.00 ||
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00 ||
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Standard Ligation || LSK || $160.00 || $176.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00 ||
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00 ||
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00 ||
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00 ||
|-
| Direct RNA || RNA004 || $160.00 || $176.00 ||
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| DNA || 96 || $700.00 || $770.00 ||
|-
| RNA || 96 || $800.00 || $880.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| colspan="5" | '''Assisted Service''' ||
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION'' ||
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00 ||
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq'' ||
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)'' ||
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION'' ||
|-
| per session || <24h notice || $200.00 || $220.00 ||
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Slide Only || per slide || $150.00 || $165.00 ||
|-
| Optimization Only || per slide || $800.00 || $880.00 ||
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00 ||
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00 ||
|-
| Antibody Add-on || per slide || $360.00 || $396.00 ||
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00 ||
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:''' ||
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00 ||
|-
| FFPE Curl || per curl || $60.00 || $66.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $300.00 || $330.00 ||
|-
| || add Quench || $100.00 || $110.00 ||
|}

== REAL TIME PCR ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| Roche LightCycler 480 || $15.00 || per plate || [[BioMicroCenter:Forms#Calenders|CALENDAR]] *available for CORE only || Contact [[BioMicroCenter:People|Christopher Hallee]] for training
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || || Available for MIT only
|-
| 96-well PLATES || $10.00 || per plate || || Available for MIT only
|}

.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR''' ||
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP''' ||
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA ||
|-
| Training || $25.00 / $27.50 || per session || TBA || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="5" | '''PLATE READER''' ||
|-
| Varioskan || $18.00 / $20.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="5" | '''ROBOTICS''' ||
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| non-filter tips || $8.00 / $8.80 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $12.00 / $13.20 || per box || ||
|-
| Training || $65.00 / $71.50 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]] ||
|-
| colspan="5" | '''NANODROP''' ||
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
|}

BioMicroCenter:Pricing

2026-04-14T15:46:33Z

Cstjohn: /* SEQUENCING - HIGH OUTPUT */

{{BioMicroCenter}}

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| NovaSeq SP 100nt || $5,178.60 || Per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq SP 300nt** || $7,333.20 ||
|-
| NovaSeq SP 500nt || $9,828.00 ||
|-
| NovaSeq S1 100nt** || $8,164.80 ||
|-
| NovaSeq S1 200nt** || $10,432.80 ||
|-
| NovaSeq S1 300nt** || $11,377.80 ||
|-
| NovaSeq S2 100nt** || $14,553.00 ||
|-
| NovaSeq S2 200nt** || $18,144.00 ||
|-
| NovaSeq S2 300nt** || $18,900.00 ||
|-
| NovaSeq S4 50nt || $5,292.00 || Per Lane || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq S4 300nt || $7,560.00 ||
|-
| NovaSeqX 10B 150PE Lane^^ || $2,590.00 || Per Lane || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| NovaSeqX 25B 150PE Lane^^ || $3,640.00 ||
|-
| NovaSeqX 10B 300nt Flowcell^^ || $17,024.00 || Per Flowcell ||
|-
| NovaSeqX 25B 300nt Flowcell^^ || $22,680.00 ||
|-
| NovaSeqX 25B 100nt Flowcell^^ || $8,050.00 ||
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,660.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,400.00 || per lane ||
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,710.00 || per flowcell ||
|-
| || One lane (~400M reads) || $1,925.00 || per lane ||
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,900.00 || per lane ||
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $420.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $2,520.00 || per flowcell ||
|-
| 150 PE: F3 Lane (~100M reads) || $630.00 || per lane ||
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
! Length !! Reads !! !! !!
|-
| 300nt || 5M || $1,001.00 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,456.00 ||
|-
| 100nt || 25M || $1,295.00 ||
|-
| 300nt || 25M || $1,867.60 ||
|-
| 600nt || 25M || $2,198.00 ||
|-
| '''1000nt''' || 25M || $3,626.00 ||
|-
| 100nt || 100M || $2,156.00 ||
|-
| 300nt || 100M || $2,975.00 ||
|-
| 600nt || 50M || $2,975.00 ||
|}

==== MiSeq i100 Walkup ====

Walkup service is not available to Non-MIT users.

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,848.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $2,002.00 ||
|-
| 300nt V2 kit (eg. 150PE) || $2,233.00 ||
|-
| 500nt V2 kit (eg. 250PE) || $2,464.00 ||
|-
| 600nt V3 kit (eg. 300PE) || $3,080.00 ||
|-
| 500nt '''NANO''' Run (eg. 250PE) || $1,078.00 ||
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 72 hour Flowcell || $1,680.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 72 hour Flowcell || pricing in ilabs || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="5" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| LIGATION BASED NEB UltraII || STD || per sample || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="5" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. ||
|-
| HTL NEB UltraII || HTL || 24 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,520.00 ||
|-
| Tagmentation Nextera-XT || STD || per Sample || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $235.00 ||
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,520.00 ||
|-
| 16S Amplicon || HTL || 48 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $910.00 ||
|-
| Other Amplicon || HTL || 48 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $945.00 ||
|-
| colspan="5" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $84.00 ||
|-
| Sample Cleanup || || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="5" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="5" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. ||
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $4,200.00 ||
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,940.00 ||
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $7,000.00 ||
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,800.00 ||
|-
| || || 96 || $4,480.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $7,000.00 ||
|-
| colspan="5" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $84.00 ||
|-
| Sample Cleanup || || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Standard Ligation || LSK || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $70.00 ||
|-
| SMARTseq -> Ligation || Cv4+LSK || $450.00 ||
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $225.00 ||
|-
| Long Insert Prep || RAD (modified, target >100kb) || $393.00 ||
|-
| Direct RNA || RNA004 || $240.00 ||
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| DNA || 96 || $980.00 ||
|-
| RNA || 96 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="4" | '''Assisted Service''' ||
|-
| colspan="4" | ''DIGITAL GENE EXPRESSION'' ||
|-
| 5' RNA / 3' RNA - setup || setup || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $5,460.00 ||
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="4" | ''scATACseq'' ||
|-
| ATACseq - setup || setup || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="4" | ''Multiome (ATAC+3'RNA)'' ||
|-
| MULTI - setup || setup || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="4" | ''CANCELLATION'' ||
|-
| per session || <24h notice || $280.00 ||
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Test Array || slide || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Slide Only || per slide || $210.00 ||
|-
| Optimization Only || per slide || $1,120.00 ||
|-
| Teton Panel || per slide || $10,080.00 ||
|-
| Atlas Short || per slide || $7,700.00 ||
|-
| Antibody Add-on || per slide || $504.00 ||
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $21.00 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $42.00 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $70.00 ||
|-
| qPCR only (Short read quantification test) || per sample || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="4" | '''Sample QC services:''' ||
|-
| Fresh Frozen Curl || per curl || $66.00 ||
|-
| FFPE Curl || per curl || $84.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Dr.Oligo || Assisted || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $420.00 ||
|-
| || add Quench || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to Non-MIT users.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR''' ||
|-
| COVARIS E220 || $28.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP''' ||
|-
| Gel Isolation || $105.00 || per gel (5 lanes) || TBA ||
|-
| Training || $50.00 || per session || TBA || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="5" | '''PLATE READER''' ||
|-
| Varioskan || $27.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="5" | '''ROBOTICS''' ||
|-
| Tecan EVO 150 || $105.00 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| non-filter tips || $11.20 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $16.80 || per box || ||
|-
| Training || $91.00 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]] ||
|-
| colspan="5" | '''NANODROP''' ||
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Informatics Support || per hour || $205.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || N/A || MIT only
|-
| Ingenuity Pathway Analysis || per dataset || $150.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || N/A ||
|-
| Tape Archive / Recovery || per tape || N/A ||
|-
| Informatics Training Classes || per session || N/A ||
|}

== SEQUENCING - MID OUTPUT ==
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason. 
=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===
{| class="wikitable" border=1
! ELEMENT AVITI SEQUENCING
!width=80| Lane/Flowcell
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=80| unit
! Notes
|-
|rowspan=2| 75 PE
| Full flowcell (2 lane, 800m read)
|align="right"| $1,900
|align="right"| $2,090
|align="right"| $2,660
|align="center"| per flowcell
|rowspan=5| Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
|One lane (~400m read)
|align="right"| $1,000
|align="right"| $1,100
|align="right"| $1,400
|align="center"| per lane
|-
|rowspan=2| 150 PE
| Full flowcell (2 lane, ~800m read)
|align="right"| $2,650
|align="right"| $2,915
|align="right"| $3,710
|align="center"| per flowcell
|-
|One lane (~400m read)
|align="right"| $1,375
|align="right"| $1,513
|align="right"| $1,925
|align="center"| per lane
|-
| 300 PE
| Full flowcell (~200m reads)
|align="right"| $3,500
|align="right"| $3,850
|align="right"| $4,900
|align="center"| per lane
|}


== SEQUENCING - LOW OUTPUT ==
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason. 
=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===
{| class="wikitable" border=1
! SINGULAR G4 SEQUENCING
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=80| unit
! Notes
|-
|50 PE: F3 Lane (~100m reads)
|align="right"| $300
|align="right"| $330
|align="right"| $420
|align="center" | per lane
|rowspan=3| Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
|Rapid 100nt: F3 flowcell (~400m reads)*
|align="right"| $1200
|align="right"| $1321
|align="right"| $1680
|align="center"| per flowcell
|-
|150 PE: F3 Lane (~100m reads)
|align="right"| $450
|align="right"| $495
|align="right"| $630
|align="center"| per lane

|}
* Rapid flowcells are required for adapted Illumina libraries.

=== MISEQ SEQUENCING ===
==== MiSeq i100 Assisted ====
{| class="wikitable" border=1
!colspan="2"| MISEQ i100 SEQUENCING
!rowspan=2 width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!rowspan=2 width=80| MIT
!rowspan=2 width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!rowspan=2 width=80| unit
!rowspan=2| Notes
|-
!Length
!Reads
|-
|300nt
|5M
|align="right"| $715
|align="right"| $786.5
|align="right"| $1001
|align="center" rowspan=9| per flowcell
|rowspan=9| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
|600nt
|5M
|align="right"| $1040
|align="right"| $1144
|align="right"| $1456
|-
|100nt
|25M
|align="right"| $925
|align="right"| $1017.5
|align="right"| $1295
|-
|300nt
|25M
|align="right"| $1334
|align="right"| $1467.4
|align="right"| $1867.6
|-
|600nt
|25M
|align="right"| $1570
|align="right"| $1727
|align="right"| $2198
|-
|1000nt
|25M
|align="right"| $2590
|align="right"| $2849
|align="right"| $3626
|-
|100nt
|100M
|align="right"| $1540
|align="right"| $1694
|align="right"| $2156
|-
|300nt
|100M
|align="right"| $2125
|align="right"| $2337.5
|align="right"| $2975
|-
|600nt
|50M
|align="right"| $2125
|align="right"| $2337.5
|align="right"| $2975
|}

==== MiSeq i100 Walkup ====
{| class="wikitable" border=1
!colspan="2"| MISEQ i100 SEQUENCING
!rowspan=2 width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!rowspan=2 width=80| MIT
!rowspan=2 width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!rowspan=2 width=80| unit
!rowspan=2 width=80| RunTime
!rowspan=2| Notes
|-
!Length
!Reads
|-
|colspan=2 | Machine usage
|align="right"| $120
|align="right"| $132
|align="right"| NA
|
|align="center"| per flowcell
| rowspan=10| training and lab storage on BMC servers required.
|-
|300nt
|5M
|align="right"| $381
|align="right"| $419.1
|align="right"| NA
|align="center"| 8h
|align="center" rowspan=9| per kit
|-
|600nt
|5M
|align="right"| $665
|align="right"| $731.5
|align="right"| NA
|align="center"| 16h
|-
|100nt
|25M
|align="right"| $565
|align="right"| $621.5
|align="right"| NA
|align="center"| 5h
|-
|300nt
|25M
|align="right"| $920
|align="right"| $1012
|align="right"| NA
|align="center"| 8h
|-
|600nt
|25M
|align="right"| $1125
|align="right"| $1238
|align="right"| NA
|align="center"| 16h
|-
|1000nt
|25M
|align="right"| $1921
|align="right"| $2113.1
|align="right"| NA
|align="center"| 24h
|-
|100nt
|100M
|align="right"| $1130
|align="right"| $1243
|align="right"| NA
|align="center"| 5h
|-
|300nt
|100M
|align="right"| $1638.5
|align="right"| $1802.35
|align="right"| NA
|align="center"| 8h
|-
|600nt
|50M
|align="right"| $1638.5
|align="right"| $1802.35
|align="right"| NA
|align="center"| 16h
|}

==== MiSeq classic ====
* MiSeq is off contract and will be retired upon instrument failure
{| class="wikitable" border=1
! MISEQ SEQUENCING
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=80| unit
! Notes
|-
|70nt V2 kit (eg. 30PE or 70SE)
|align="right"| $1,320
|align="right"| $1,452
|align="right"| $1,848
|align="center" rowspan=6| per lane
|rowspan=6| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
|150nt V3 kits (eg. 75PE)
|align="right"| $1,430
|align="right"| $1,573
|align="right"| $2,002
|-
|300nt V2 kit (eg. 150PE)
|align="right"| $1,595
|align="right"| $1,754.50
|align="right"| $2,233
|-
|500nt V2 kit (eg. 250PE)
|align="right"| $1,760
|align="right"| $1,936
|align="right"| $2,464
|-
|600nt V3 kit (eg. 300PE)
|align="right"| $2,200
|align="right"| $2,420
|align="right"| $3,080
|-
|500nt '''NANO''' Run (eg. 250PE)
|align="right"| $770
|align="right"| $847
|align="right"| $1,078
|}

=== OTHER SHORT READ SERVICES ===
{| class="wikitable" border=1
! SHORT READ SUPPORTING SERVICES
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=80| unit
! Notes
|-
| Quality Control Only -only one QC is included per sequencing lane.
|align="right"| $35
|align="right"| $38.50
|align="right"| $49
|align="center"| per sample
| Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling.
|align="right"| $15
|align="right"| $16.50
|align="right"| $21
|align="center"| per sample
| Includes Fluorometric quantification and Frag.Analyzer
|-
|qPCR only (short read)
|align="right"| $25
|align="right"| $27.50
|align="right"| $35
|align="center"| per sample
| Includes qPCR values from standard QC only
|-
|qPCR only - full plate(short read)
|align="right"| $400
|align="right"| $440
|align="right"| $560
|align="center"| per sample
| excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable" border=1
! PROMETHION SEQUENCING
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=80| unit
! Notes
|-
| 72 hour Flowcell
|align="right"| $1,200
|align="right"| $1,320
|align="right"| $1,680
|align="center"|per Flowcell
||Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable" border=1
! PACBIO REVIO SEQUENCING^^
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=80| unit
! Notes
|-
| 72 hour Flowcell
|colspan=3 | pricing in ilabs
|align="center"|per Flowcell
||Fastq stored for 1y. Trace data stored only 30d.
|}

^^ - through collaboration with nearby academic shared resources

== LIBRARY GENERATION ==
=== SHORT READ LIBRARY - CONVERSION ===
{| class="wikitable" border=1
!width=100| Input library
!width=100| Convert To
!width=100| Method
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|align="center"|Illumina
|align="center"|Singular
|align="center"|Index Overwrite (per sample)
|align="right"| $30
|align="right"| $33
|align="right"| $42
| Includes QC and reindexing
|-
|align="center"|Illumina
|align="center"|Singular
|align="center"|P5/P7 conversion (per pool)
|align="right"| $100
|align="right"| $110
|align="right"| $140
| Includes QC. Samples not reindexed Requires full flowcell for sequencing (Rapid) 
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable" border=1
!width=160| ILLUMINA Sample Preparation
!width=60| Type
!width=60| Batch Size
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
! colspan="4" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
! colspan="3" style="color: blue;" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
|rowspan=3 |LIGATION BASED NEB UltraII
|align="center"|STD
|align="center"| per sample
|align="right"| $105
|align="right"| $115
|align="right"| $157
| Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|- style="color: blue;"
|align="center" rowspan=2|HTL
|align="center"| 24
|align="right"| $900
|align="right"| $990
|align="right"| $1,260
|rowspan=2 | Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|- style="color: blue;"
|align="center"| 96
|align="right"| $1,800
|align="right"| $1,980
|align="right"| $2,520
|-

|Tagmentation NEXTERA-XT
|align="center" rowspan=2|STD
|align="center" rowspan=2|per Sample
|align="right"| $105
|align="right"| $115
|align="right"| $157
| rowspan="2" | Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
|Tagmentation NEXTERA-FLEX
|align="right"| $157
|align="right"| $173
|align="right"| $236

|- style="color: blue;"
|rowspan=2 | NEXTERA-XT OR FLEX
|rowspan=2 align="center"| HTL
|align="center"| ''24''
|align="right"| $900
|align="right"| $990
|align="right"| $1,260
|rowspan=2 | Includes tagmentation, indexing, enrichment, and quality control spotcheck(SYBR and FA).
|-
|align="center" style="color: blue;" | ''96''
|align="right" style="color: blue;" | $1,800
|align="right" style="color: blue;" | $1,980
|align="right" style="color: blue;" | $2,520

|-style="color: blue;"
|rowspan="2" | 16S Amplicon
|align="center" rowspan=2 | HTL
|align="center"|48
|align="right" | $1,900
|align="right" | $2,090
|align="right" | $2,660
|rowspan="2" | Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-style="color: blue;"
|align="center"|add'l 48
|align="right" | $650
|align="right" | $715
|align="right" | $910

|-style="color: blue;"
|rowspan="2" | Other Amplicon
|rowspan=2 align="center"| HTL
|align="center" |48
|align="right" | $1,600
|align="right" | $1,760
|align="right" | $2,240
|rowspan="2" | Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|- style="color: blue;"
|align="center"|add'l 48
|align="right" | $630
|align="right" | $693
|align="right" | $945
|-
! colspan="7" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
|style="color: blue;" colspan=2| High Throughput Setup
|align="center" style="color: blue;" |48 samples
|align="right" style="color: blue;" | $60
|align="right" style="color: blue;" | $66
|align="right" style="color: blue;" | $84
|
|-
|colspan=2|Sample Cleanup
|align="center"|per sample
|align="right"| $5
|align="right"| $5.50
|align="right"| $7
|SPRI cleanup of samples to remove impurities.
|-
|style="color: blue;" colspan=2|Quantification
|align="center" style="color: blue;" |per sample
|align="right" style="color: blue;" | $10
|align="right" style="color: blue;" | $11
|align="right" style="color: blue;" | $14
|Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable" border=1
!width=160| ILLUMINA Sample Preparation
!width=60| Type
!width=60| Batch Size
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
! colspan="4" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
! colspan="3" style="color: blue;" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
|rowspan="3"|polyA based isolation NEB UltraII RNAseq
|align="center"|STD
|align="center"| per sample
|align="right"| $157
|align="right"| $173
|align="right"| $236
|Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
|rowspan=2 align="center" style="color: blue;"| HTL (NEB only)
|align="center" style="color: blue;" | ''24''
|align="right" style="color: blue;" | $1,500
|align="right" style="color: blue;" | $1,650
|align="right" style="color: blue;" | $2,100
|Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
|align="center" style="color: blue;" | ''96''
|align="right" style="color: blue;" | $3,000
|align="right" style="color: blue;" | $3,300
|align="right" style="color: blue;" | $4,200
|Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-

|rowspan="2"| High Throughput 3' Digital Gene Expression (HT3DGE)
|rowspan=2 align="center" style="color: blue;"| HTL
|align="center" style="color: blue;"| ''24''
|align="right" style="color: blue;" | $1,050
|align="right" style="color: blue;" | $1,155
|align="right" style="color: blue;" | $1,470
| rowspan="2" style="color: blue;" | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
|align="center" style="color: blue;" | ''96''
|align="right" style="color: blue;" | $2,100
|align="right" style="color: blue;" | $2,310
|align="right" style="color: blue;" | $2,940
|-

|rowspan="3"|Total RNA sequencing NEB RNAseq +Ribodepletion
|align="center"|STD
|align="center"| per sample
|align="right"| $235
|align="right"| $258.5
|align="right"| $352.5
|Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
|rowspan=2 align="center" style="color: blue;"| HTL (NEB only, covaris RNA fragmentation)
|align="center" style="color: blue;" | ''24''
|align="right" style="color: blue;" | $2,500
|align="right" style="color: blue;" | $2,750
|align="right" style="color: blue;" | $3,500
|rowspan=2|Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
|align="center" style="color: blue;" | ''96''
|align="right" style="color: blue;" | $5,000
|align="right" style="color: blue;" | $5,500
|align="right" style="color: blue;" | $7,000
|-

|rowspan=3|Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex
|align="center"| '''STD''' v4
|align="center"|per sample
|align="right"| $262
|align="right"| $289
|align="right"| $394
| rowspan="3" | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
|rowspan="2" align="center" style="color: blue;"| '''HTL''' v2
|align="center" style="color: blue;" | ''24''
|align="right" style="color: blue;" | $2,000
|align="right" style="color: blue;" | $2,200
|align="right" style="color: blue;" | $2,800
|-
|align="center" style="color: blue;" | ''96''
|align="right" style="color: blue;" | $3,200
|align="right" style="color: blue;" | $3,520
|align="right" style="color: blue;" | $4,480
|-

|rowspan="3"|Low Input Mammalian Total Clontech ZapR Kit
|align="center"| STD
|align="center"| per sample
|align="right"| $210
|align="right"| $231
|align="right"| $315
| rowspan="1" | Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
|rowspan=2 align="center" style="color: blue;"| HTL (covaris RNA fragmentation)
|align="center" style="color: blue;" | ''24''
|align="right" style="color: blue;" | $3000
|align="right" style="color: blue;" | $3300
|align="right" style="color: blue;" | $4200
|rowspan=2|Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
|align="center" style="color: blue;" | ''96''
|align="right" style="color: blue;" | $5000
|align="right" style="color: blue;" | $5500
|align="right" style="color: blue;" | $7000
|-
| Small RNA
|align="center"| STD
| align="center"|per sample
| align="right"|$289
| align="right"|$318
| align="right"|$433
| Uses NEB, BIOO or QIAGEN small RNA kit
|-
! colspan="7" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
|colspan=2 style="color: blue;" | High Throughput Setup
|align="center" style="color: blue;" |48 samples
|align="right" style="color: blue;" | $60
|align="right" style="color: blue;" | $66
|align="right" style="color: blue;" | $84
|
|-
|colspan=2|Sample Cleanup
|align="center"|per sample
|align="right"| $5
|align="right"| $5.50
|align="right"| $7
|SPRI cleanup of samples to remove impurities.
|-
|colspan=2 style="color: blue;" |Quantification
|align="center" style="color: blue;" |per sample
|align="right" style="color: blue;" | $10
|align="right" style="color: blue;" | $11
|align="right" style="color: blue;" | $14
|Quantification of samples (FA) prior to arraying.
|-
|}

.

=== LONG READ LIBRARIES ===
{| class="wikitable" border=1
!Oxford Nanopore Sample Preparation
!width=120| Nanopore Kit
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|Standard Ligation || LSK || $160 || $176 || $240
|rowspan=6|Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
|Native Barcoding (ligation indexing) || - || $50 || $55 || $70
|-
|SMARTseq -> Ligation || Cv4+LSK || $300 || $330 || $420
|-
|Rapid Prep (tagment - ~10-20kb) || RAD || $150 || $165 || $225
|-
|Long Insert Prep || RAD (modified, target >100kb) || $262 || $289 || $393
|-
|Direct RNA || RNA004 || $160 || $176 || $240
|}

== SAMPLE EXTRACTION ==
=== CHEMAGIC360 ===
{| class="wikitable" border=1
!TYPE
!width=120| Batch Size
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|DNA
|96
|$700
|$770
|$980
|
|-
|RNA
|96
|$800
|$880
|$1120
|
|}

== SINGLE CELL & SPATIAL SERVICES ==
=== 10X CHROMIUM ===
{| class="wikitable" border=1
! 10X USAGE
!width=120| Batch Size
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|colspan=6 align="center"|'''Assisted Service'''
|-
|colspan=6 |''DIGITAL GENE EXPRESSION''
|-
|rowspan=2 |5' RNA 3' RNA
|setup || $1,015 || $1,116.50 || $1,421 || charge per submission
|-
|per sample|| $1,750 || $1,925 || $2,450 || Includes QC of cDNA and creation of Short Read Library
|-
|rowspan=2 |5' RNA 3' RNA - on chip multiplexing
|setup || $1,150 || $1,265 || $1,1610 || charge per submission
|-
|per 4 samples|| $2,305 || $2,535 || $3,227 || Includes QC of cDNA and creation of Short Read Library
|-
|rowspan=2 |Fixed RNA (Flex v2) probe based. human/mouse only
|setup || $2700 || $2970 || $3780 || charge per submission - upto 48 samples
|-
|per sample || $270|| $297 || $378 || Includes QC of cDNA and creation of Short Read Library
|-
|rowspan=2 |Amplicon (CITE-SEQ, TCR, BCR, antibody barcocde, etc // per amplicon)
|setup || $470 || $517 || $658 || additional prep beyond DGE
|-
|per sample|| $45 || $50 || $63 || Includes QC of cDNA and creation of Short Read Library
|-
|colspan=6 |''scATACseq''
|-
|rowspan=2 |ATACseq
|setup || $880 || $968 || $1,232 || charge per submission
|-
|per sample|| $1,675|| $1,843|| $2,345|| Includes QC and creation of Short Read Library
|-
|colspan=6 |''Multiome (ATAC+3'RNA)''
|-
|rowspan=2 |MULTI
|setup || $1,800|| $1,980|| $2,520|| charge per submission
|-
|per sample|| $3,100|| $3,410|| $4,340|| Includes QC of cDNA and creation of Short Read Library
|-
|colspan=6 |''CANCELLATION''
|-
|per session || || $200|| $220|| $280|| <24h notice
|}
*please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===
{| class="wikitable" border=1
! SLIDES
!width=120| Batch Size
!width=80| KI Only
!width=80| [[BioMicroCenter:CoreDeps|Other CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|Test Array ||slide|| $1,300|| $1,511|| $1,607|| $2435|| recommended 1x per tissue type for fresh frozen
|-
|Sample Slide - Fresh Frozen ||1 slide / 4 tiles|| $6,210|| $6,340|| $6,940|| $9,067 || Includes H&E stain images + Library Generation
|-
|Sample Slide - FFPE (hs/mm only) ||1 slide / 2 tiles|| $4,550|| $4,550|| $4,996|| $6,359 || Includes library generation only.
|-
|Sample Slide - HD (hs/mm only) ||1 slide / 2 tiles|| $7,850|| $7,850|| $8,596|| $10,940 || Includes library generation only.
|}

=== AVITI24 ===
<div style="background:#fff8e6; border-left:4px solid #c8960c; padding:8px 12px; margin-bottom:8px;">'''Beta Service — Early Access:''' Consultation with BMC staff is required before submitting samples. See [[BioMicroCenter:SpTx|Spatial Transcriptomics]] for full service details.</div>
{| class="wikitable" border=1
! AVITI24 CYTOPROFILING / DISS
!width=80|UNIT
!width=80|[[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80|MIT
!width=80|[[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|Atlas Panel (Teton CytoProfiling)
|rowspan=5 align=center| per slide 1-, 12-, 48-well
|align=right| $7,200
|align=right| $7,920
|align=right| $10,080
|RNA + protein + morphology
|-
|Atlas Low (DISS — targeted in situ sequencing)
|align=right| $5,500
|align=right| $6,050
|align=right| $7,700
|User-designed probe; can run simultaneously with Atlas Panel
|-
|Antibody Add-on
|align=right| $360
|align=right| $396
|align=right| $504
|Custom antibody targets; consult BMC staff
|-
|Optimization Run
|align=right| $800
|align=right| $880
|align=right| $1,120
|Charged only if optimization slide fails
|-
|Slide Only (consumable)
|align=right| $150
|align=right| $165
|align=right| $210
|
|}
* High-output DISS (whole transcriptome) not yet released — contact BMC staff.

== OTHER GENOMICS SERVICES ==

 

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===
{| class="wikitable" border=1
! QUALITY CONTROL
!width=100| unit
!width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=100| MIT
!width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|Fragment Analyzer: RNA or DNA
|per sample ||$15 || $16.50 || $21
|
|-
|Fragment Analyzer: Preloaded row
| per row (12) ||$80||$88 || $112
| 2ul per sample in each well using plates from BMC
|-
|FemtoPulse: RNA or DNA
|per sample || $30 || $33 || $42
|
|-
|FemtoPulse: Preloaded Row
|per row (12) || $160 || $176 || $224
| 2ul per sample in each well using plates from BMC
|-
|BioAnalyzer: Walkup Service
|per chip|| $50 || $55 || $70
|
|-
|qPCR only (Short read quantification test)
|per sample || $25 || $27.50 || $35
| 4 point serial dilution
|-
|Full plate qPCR (short read quantification test)
|per plate || $400 || $440 || $560
| full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
|colspan=6 align="center"| Sample QC services:
|-
|Fresh Frozen Curl
|rowspan=2|per curl
| $40|| $44 || $56
|
|-
|FFPE Curl
| $60 || $66 || $84
|
|-
|}

=== OLIGO SYNTHESIS ===
{| class="wikitable" border=1
!Platform
!width=100| Service
!width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=100| MIT
!width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|rowspan=3| Dr.Oligo
|Assisted
|$2000 +consumables
|$2200 +consumables
|$2800 +consumables
|includes setup and run of the instrument only. Large reagents / N2 included.
|-
|Walkup
|$1000 +consumables
|$1100 +consumables
|$1400 +consumables
|includes run of the instrument only. Large reagents / N2 included.
|-
|Training
|$4000
|$4400
|$5600
|ncludes two instrument runs.
|-
|rowspan=6| DNAscript Syntax
|Setup (per run)
|$1000
|$1100
|$1400
|Per run cost
|-
|2000 nucleotide synthesis
|$500
|$550
|$700
|Up to 96 samples
|-
|8000 nucleotide synthesis
|$1500
|$1650
|$2100
|Up to 96 samples
|-
|add Biotin (max48)
|$115
|$126
|$161
|rowspan = 3| max 48 per run and 2 per oligo. Couple to T only.
|-
|add Fluor
|$300
|$330
|$420
|-
|add quench
|$100
|$110
|$140
|}

== [[BioMicroCenter:RTPCR|REAL TIME PCR]] ==

{| border=1
! OTHER LAB SERVICES
!width=100| [[BioMicroCenter:CoreDeps|CORE LAB / MIT]]
!width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=100| unit
! SIGN UP
! Notes
|-
| Roche LightCycler 480
|align="right"| $15
|align="right"| N/A
|align="center"| per plate
| [[BioMicroCenter:Forms#Calenders|CALENDAR]] *available for CORE only
| Contact [[BioMicroCenter:People|Christopher Hallee]] for training
|-
| SYBR Green (KAPA/Roche)
|align="right"| $45
|align="right"| N/A
|align="center"| per ml
|
| Available for MIT only
|-
| 96-well PLATES
|align="right"| $10
|align="right"| N/A
|align="center"| per plate
|
| Available for MIT only
|-
|}
.
 

== OTHER LAB SERVICES ==
{| border=1
! OTHER LAB SERVICES
!width=100| [[BioMicroCenter:CoreDeps|CORE LAB / MIT]]
!width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=100| unit
! SIGN UP
! Notes
|-
|colspan=6 align="center"| '''COVARIS SONICATOR'''
|-
|COVARIS E220
|align="right"|$20
|align="right"|$30
|per hour
|[https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar]
|tubes available for purchase.
|-

| colspan="6" align="center"| '''PIPPIN PREP'''
|-
|Gel Isolation
|align="right"| $75
|align="right"| $105
|align="center"| per gel (5 lanes)
| TBA
|
|-
|Training
|align="right"| $25
|align="right"| $50
|align="center"| per session
| TBA
| Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="6" align="center"| '''PLATE READER'''
|-
| Varioskan
|align="right"| $18
|align="right"| $27
|align="center"| per hour
| [[BioMicroCenter:Forms#Calenders|CALENDAR]]
| Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="6" align="center"| '''ROBOTICS'''
|-
| Tecan EVO 150
|align="right"| $75
|align="right"| $105
|align="center"| per 1h block - based on scheduled time.
| [[BioMicroCenter:Forms#Calenders|CALENDAR]]
|
|-
|non-filter tips
|align="right"| $8
|align="right"| $11.20
|align="center"| per box
|
|** Please let us know if there is additional plasticware you would like us to stock.
|-
|Filter tips
|align="right"| $12.00
|align="right"| $16.80
|align="center"| per box
|
|
|-
| Training
|align="right"| $65
|align="right"| $91
|align="center"| per hour
| Contact [[BioMicroCenter:People|Tatum Murdock]]
|
|-
| colspan="6" align="center"| '''NANODROP'''
|-
| Nanodrop
|align="right"| --
|align="right"| --
|align="center"|
| Walk up service
| * no charge
|-
|}
 

== COMPUTATIONAL SERVICES ==
{| class="wikitable" border=1
! COMPUTATIONAL SERVICES
!width=200| unit
!width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=100| Other MIT / non-MIT
! Notes
|-
| Informatics Support
| align="right"| per hour
| align="right"| $90
| align="right"| $125 / $205
| Very limited support for non-MIT users
|-
| Informatics Share
| align="right"| per 0.1FTE
| align="right"| $1,250
| align="right"| N/A
| MIT only. Priority access to informatics.
|-
|Ingenuity Pathway Analysis
|align="right"|per dataset
|align="right" | $100
|align="right" | $110/$150
| Recovery of licence cost.
|-
|Active Data storage
|align="right"| per TB per year
|align="right"| $100
|align="right"| $300 / N/A
|
|-
| Tape Archive / Recovery
|align="right"| per tape
|align="right"| $60
|align="right"| $70
|
|-
| Informatics Training Classes
|align="right"| per session
|align="right"| $50
|align="right"| $100
|
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

BioMicroCenter:Pricing

2026-04-14T15:46:20Z

Cstjohn: /* NOVASEQX / NOVASEQ6000 SEQUENCING */

{{BioMicroCenter}}

== SEQUENCING - HIGH OUTPUT ==
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason. 

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| NovaSeq SP 100nt || $5,178.60 || Per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq SP 300nt** || $7,333.20 ||
|-
| NovaSeq SP 500nt || $9,828.00 ||
|-
| NovaSeq S1 100nt** || $8,164.80 ||
|-
| NovaSeq S1 200nt** || $10,432.80 ||
|-
| NovaSeq S1 300nt** || $11,377.80 ||
|-
| NovaSeq S2 100nt** || $14,553.00 ||
|-
| NovaSeq S2 200nt** || $18,144.00 ||
|-
| NovaSeq S2 300nt** || $18,900.00 ||
|-
| NovaSeq S4 50nt || $5,292.00 || Per Lane || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq S4 300nt || $7,560.00 ||
|-
| NovaSeqX 10B 150PE Lane^^ || $2,590.00 || Per Lane || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| NovaSeqX 25B 150PE Lane^^ || $3,640.00 ||
|-
| NovaSeqX 10B 300nt Flowcell^^ || $17,024.00 || Per Flowcell ||
|-
| NovaSeqX 25B 300nt Flowcell^^ || $22,680.00 ||
|-
| NovaSeqX 25B 100nt Flowcell^^ || $8,050.00 ||
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,660.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,400.00 || per lane ||
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,710.00 || per flowcell ||
|-
| || One lane (~400M reads) || $1,925.00 || per lane ||
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,900.00 || per lane ||
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $420.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $2,520.00 || per flowcell ||
|-
| 150 PE: F3 Lane (~100M reads) || $630.00 || per lane ||
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
! Length !! Reads !! !! !!
|-
| 300nt || 5M || $1,001.00 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,456.00 ||
|-
| 100nt || 25M || $1,295.00 ||
|-
| 300nt || 25M || $1,867.60 ||
|-
| 600nt || 25M || $2,198.00 ||
|-
| '''1000nt''' || 25M || $3,626.00 ||
|-
| 100nt || 100M || $2,156.00 ||
|-
| 300nt || 100M || $2,975.00 ||
|-
| 600nt || 50M || $2,975.00 ||
|}

==== MiSeq i100 Walkup ====

Walkup service is not available to Non-MIT users.

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,848.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $2,002.00 ||
|-
| 300nt V2 kit (eg. 150PE) || $2,233.00 ||
|-
| 500nt V2 kit (eg. 250PE) || $2,464.00 ||
|-
| 600nt V3 kit (eg. 300PE) || $3,080.00 ||
|-
| 500nt '''NANO''' Run (eg. 250PE) || $1,078.00 ||
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 72 hour Flowcell || $1,680.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 72 hour Flowcell || pricing in ilabs || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="5" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| LIGATION BASED NEB UltraII || STD || per sample || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="5" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. ||
|-
| HTL NEB UltraII || HTL || 24 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,520.00 ||
|-
| Tagmentation Nextera-XT || STD || per Sample || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $235.00 ||
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,520.00 ||
|-
| 16S Amplicon || HTL || 48 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $910.00 ||
|-
| Other Amplicon || HTL || 48 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $945.00 ||
|-
| colspan="5" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $84.00 ||
|-
| Sample Cleanup || || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="5" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="5" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. ||
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $4,200.00 ||
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,940.00 ||
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $7,000.00 ||
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,800.00 ||
|-
| || || 96 || $4,480.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $7,000.00 ||
|-
| colspan="5" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $84.00 ||
|-
| Sample Cleanup || || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Standard Ligation || LSK || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $70.00 ||
|-
| SMARTseq -> Ligation || Cv4+LSK || $450.00 ||
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $225.00 ||
|-
| Long Insert Prep || RAD (modified, target >100kb) || $393.00 ||
|-
| Direct RNA || RNA004 || $240.00 ||
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| DNA || 96 || $980.00 ||
|-
| RNA || 96 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="4" | '''Assisted Service''' ||
|-
| colspan="4" | ''DIGITAL GENE EXPRESSION'' ||
|-
| 5' RNA / 3' RNA - setup || setup || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $5,460.00 ||
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="4" | ''scATACseq'' ||
|-
| ATACseq - setup || setup || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="4" | ''Multiome (ATAC+3'RNA)'' ||
|-
| MULTI - setup || setup || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="4" | ''CANCELLATION'' ||
|-
| per session || <24h notice || $280.00 ||
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Test Array || slide || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Slide Only || per slide || $210.00 ||
|-
| Optimization Only || per slide || $1,120.00 ||
|-
| Teton Panel || per slide || $10,080.00 ||
|-
| Atlas Short || per slide || $7,700.00 ||
|-
| Antibody Add-on || per slide || $504.00 ||
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $21.00 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $42.00 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $70.00 ||
|-
| qPCR only (Short read quantification test) || per sample || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="4" | '''Sample QC services:''' ||
|-
| Fresh Frozen Curl || per curl || $66.00 ||
|-
| FFPE Curl || per curl || $84.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Dr.Oligo || Assisted || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $420.00 ||
|-
| || add Quench || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to Non-MIT users.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR''' ||
|-
| COVARIS E220 || $28.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP''' ||
|-
| Gel Isolation || $105.00 || per gel (5 lanes) || TBA ||
|-
| Training || $50.00 || per session || TBA || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="5" | '''PLATE READER''' ||
|-
| Varioskan || $27.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="5" | '''ROBOTICS''' ||
|-
| Tecan EVO 150 || $105.00 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| non-filter tips || $11.20 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $16.80 || per box || ||
|-
| Training || $91.00 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]] ||
|-
| colspan="5" | '''NANODROP''' ||
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Informatics Support || per hour || $205.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || N/A || MIT only
|-
| Ingenuity Pathway Analysis || per dataset || $150.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || N/A ||
|-
| Tape Archive / Recovery || per tape || N/A ||
|-
| Informatics Training Classes || per session || N/A ||
|}

== SEQUENCING - MID OUTPUT ==
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason. 
=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===
{| class="wikitable" border=1
! ELEMENT AVITI SEQUENCING
!width=80| Lane/Flowcell
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=80| unit
! Notes
|-
|rowspan=2| 75 PE
| Full flowcell (2 lane, 800m read)
|align="right"| $1,900
|align="right"| $2,090
|align="right"| $2,660
|align="center"| per flowcell
|rowspan=5| Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
|One lane (~400m read)
|align="right"| $1,000
|align="right"| $1,100
|align="right"| $1,400
|align="center"| per lane
|-
|rowspan=2| 150 PE
| Full flowcell (2 lane, ~800m read)
|align="right"| $2,650
|align="right"| $2,915
|align="right"| $3,710
|align="center"| per flowcell
|-
|One lane (~400m read)
|align="right"| $1,375
|align="right"| $1,513
|align="right"| $1,925
|align="center"| per lane
|-
| 300 PE
| Full flowcell (~200m reads)
|align="right"| $3,500
|align="right"| $3,850
|align="right"| $4,900
|align="center"| per lane
|}


== SEQUENCING - LOW OUTPUT ==
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason. 
=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===
{| class="wikitable" border=1
! SINGULAR G4 SEQUENCING
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=80| unit
! Notes
|-
|50 PE: F3 Lane (~100m reads)
|align="right"| $300
|align="right"| $330
|align="right"| $420
|align="center" | per lane
|rowspan=3| Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
|Rapid 100nt: F3 flowcell (~400m reads)*
|align="right"| $1200
|align="right"| $1321
|align="right"| $1680
|align="center"| per flowcell
|-
|150 PE: F3 Lane (~100m reads)
|align="right"| $450
|align="right"| $495
|align="right"| $630
|align="center"| per lane

|}
* Rapid flowcells are required for adapted Illumina libraries.

=== MISEQ SEQUENCING ===
==== MiSeq i100 Assisted ====
{| class="wikitable" border=1
!colspan="2"| MISEQ i100 SEQUENCING
!rowspan=2 width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!rowspan=2 width=80| MIT
!rowspan=2 width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!rowspan=2 width=80| unit
!rowspan=2| Notes
|-
!Length
!Reads
|-
|300nt
|5M
|align="right"| $715
|align="right"| $786.5
|align="right"| $1001
|align="center" rowspan=9| per flowcell
|rowspan=9| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
|600nt
|5M
|align="right"| $1040
|align="right"| $1144
|align="right"| $1456
|-
|100nt
|25M
|align="right"| $925
|align="right"| $1017.5
|align="right"| $1295
|-
|300nt
|25M
|align="right"| $1334
|align="right"| $1467.4
|align="right"| $1867.6
|-
|600nt
|25M
|align="right"| $1570
|align="right"| $1727
|align="right"| $2198
|-
|1000nt
|25M
|align="right"| $2590
|align="right"| $2849
|align="right"| $3626
|-
|100nt
|100M
|align="right"| $1540
|align="right"| $1694
|align="right"| $2156
|-
|300nt
|100M
|align="right"| $2125
|align="right"| $2337.5
|align="right"| $2975
|-
|600nt
|50M
|align="right"| $2125
|align="right"| $2337.5
|align="right"| $2975
|}

==== MiSeq i100 Walkup ====
{| class="wikitable" border=1
!colspan="2"| MISEQ i100 SEQUENCING
!rowspan=2 width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!rowspan=2 width=80| MIT
!rowspan=2 width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!rowspan=2 width=80| unit
!rowspan=2 width=80| RunTime
!rowspan=2| Notes
|-
!Length
!Reads
|-
|colspan=2 | Machine usage
|align="right"| $120
|align="right"| $132
|align="right"| NA
|
|align="center"| per flowcell
| rowspan=10| training and lab storage on BMC servers required.
|-
|300nt
|5M
|align="right"| $381
|align="right"| $419.1
|align="right"| NA
|align="center"| 8h
|align="center" rowspan=9| per kit
|-
|600nt
|5M
|align="right"| $665
|align="right"| $731.5
|align="right"| NA
|align="center"| 16h
|-
|100nt
|25M
|align="right"| $565
|align="right"| $621.5
|align="right"| NA
|align="center"| 5h
|-
|300nt
|25M
|align="right"| $920
|align="right"| $1012
|align="right"| NA
|align="center"| 8h
|-
|600nt
|25M
|align="right"| $1125
|align="right"| $1238
|align="right"| NA
|align="center"| 16h
|-
|1000nt
|25M
|align="right"| $1921
|align="right"| $2113.1
|align="right"| NA
|align="center"| 24h
|-
|100nt
|100M
|align="right"| $1130
|align="right"| $1243
|align="right"| NA
|align="center"| 5h
|-
|300nt
|100M
|align="right"| $1638.5
|align="right"| $1802.35
|align="right"| NA
|align="center"| 8h
|-
|600nt
|50M
|align="right"| $1638.5
|align="right"| $1802.35
|align="right"| NA
|align="center"| 16h
|}

==== MiSeq classic ====
* MiSeq is off contract and will be retired upon instrument failure
{| class="wikitable" border=1
! MISEQ SEQUENCING
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=80| unit
! Notes
|-
|70nt V2 kit (eg. 30PE or 70SE)
|align="right"| $1,320
|align="right"| $1,452
|align="right"| $1,848
|align="center" rowspan=6| per lane
|rowspan=6| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
|150nt V3 kits (eg. 75PE)
|align="right"| $1,430
|align="right"| $1,573
|align="right"| $2,002
|-
|300nt V2 kit (eg. 150PE)
|align="right"| $1,595
|align="right"| $1,754.50
|align="right"| $2,233
|-
|500nt V2 kit (eg. 250PE)
|align="right"| $1,760
|align="right"| $1,936
|align="right"| $2,464
|-
|600nt V3 kit (eg. 300PE)
|align="right"| $2,200
|align="right"| $2,420
|align="right"| $3,080
|-
|500nt '''NANO''' Run (eg. 250PE)
|align="right"| $770
|align="right"| $847
|align="right"| $1,078
|}

=== OTHER SHORT READ SERVICES ===
{| class="wikitable" border=1
! SHORT READ SUPPORTING SERVICES
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=80| unit
! Notes
|-
| Quality Control Only -only one QC is included per sequencing lane.
|align="right"| $35
|align="right"| $38.50
|align="right"| $49
|align="center"| per sample
| Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling.
|align="right"| $15
|align="right"| $16.50
|align="right"| $21
|align="center"| per sample
| Includes Fluorometric quantification and Frag.Analyzer
|-
|qPCR only (short read)
|align="right"| $25
|align="right"| $27.50
|align="right"| $35
|align="center"| per sample
| Includes qPCR values from standard QC only
|-
|qPCR only - full plate(short read)
|align="right"| $400
|align="right"| $440
|align="right"| $560
|align="center"| per sample
| excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable" border=1
! PROMETHION SEQUENCING
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=80| unit
! Notes
|-
| 72 hour Flowcell
|align="right"| $1,200
|align="right"| $1,320
|align="right"| $1,680
|align="center"|per Flowcell
||Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable" border=1
! PACBIO REVIO SEQUENCING^^
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=80| unit
! Notes
|-
| 72 hour Flowcell
|colspan=3 | pricing in ilabs
|align="center"|per Flowcell
||Fastq stored for 1y. Trace data stored only 30d.
|}

^^ - through collaboration with nearby academic shared resources

== LIBRARY GENERATION ==
=== SHORT READ LIBRARY - CONVERSION ===
{| class="wikitable" border=1
!width=100| Input library
!width=100| Convert To
!width=100| Method
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|align="center"|Illumina
|align="center"|Singular
|align="center"|Index Overwrite (per sample)
|align="right"| $30
|align="right"| $33
|align="right"| $42
| Includes QC and reindexing
|-
|align="center"|Illumina
|align="center"|Singular
|align="center"|P5/P7 conversion (per pool)
|align="right"| $100
|align="right"| $110
|align="right"| $140
| Includes QC. Samples not reindexed Requires full flowcell for sequencing (Rapid) 
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable" border=1
!width=160| ILLUMINA Sample Preparation
!width=60| Type
!width=60| Batch Size
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
! colspan="4" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
! colspan="3" style="color: blue;" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
|rowspan=3 |LIGATION BASED NEB UltraII
|align="center"|STD
|align="center"| per sample
|align="right"| $105
|align="right"| $115
|align="right"| $157
| Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|- style="color: blue;"
|align="center" rowspan=2|HTL
|align="center"| 24
|align="right"| $900
|align="right"| $990
|align="right"| $1,260
|rowspan=2 | Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|- style="color: blue;"
|align="center"| 96
|align="right"| $1,800
|align="right"| $1,980
|align="right"| $2,520
|-

|Tagmentation NEXTERA-XT
|align="center" rowspan=2|STD
|align="center" rowspan=2|per Sample
|align="right"| $105
|align="right"| $115
|align="right"| $157
| rowspan="2" | Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
|Tagmentation NEXTERA-FLEX
|align="right"| $157
|align="right"| $173
|align="right"| $236

|- style="color: blue;"
|rowspan=2 | NEXTERA-XT OR FLEX
|rowspan=2 align="center"| HTL
|align="center"| ''24''
|align="right"| $900
|align="right"| $990
|align="right"| $1,260
|rowspan=2 | Includes tagmentation, indexing, enrichment, and quality control spotcheck(SYBR and FA).
|-
|align="center" style="color: blue;" | ''96''
|align="right" style="color: blue;" | $1,800
|align="right" style="color: blue;" | $1,980
|align="right" style="color: blue;" | $2,520

|-style="color: blue;"
|rowspan="2" | 16S Amplicon
|align="center" rowspan=2 | HTL
|align="center"|48
|align="right" | $1,900
|align="right" | $2,090
|align="right" | $2,660
|rowspan="2" | Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-style="color: blue;"
|align="center"|add'l 48
|align="right" | $650
|align="right" | $715
|align="right" | $910

|-style="color: blue;"
|rowspan="2" | Other Amplicon
|rowspan=2 align="center"| HTL
|align="center" |48
|align="right" | $1,600
|align="right" | $1,760
|align="right" | $2,240
|rowspan="2" | Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|- style="color: blue;"
|align="center"|add'l 48
|align="right" | $630
|align="right" | $693
|align="right" | $945
|-
! colspan="7" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
|style="color: blue;" colspan=2| High Throughput Setup
|align="center" style="color: blue;" |48 samples
|align="right" style="color: blue;" | $60
|align="right" style="color: blue;" | $66
|align="right" style="color: blue;" | $84
|
|-
|colspan=2|Sample Cleanup
|align="center"|per sample
|align="right"| $5
|align="right"| $5.50
|align="right"| $7
|SPRI cleanup of samples to remove impurities.
|-
|style="color: blue;" colspan=2|Quantification
|align="center" style="color: blue;" |per sample
|align="right" style="color: blue;" | $10
|align="right" style="color: blue;" | $11
|align="right" style="color: blue;" | $14
|Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable" border=1
!width=160| ILLUMINA Sample Preparation
!width=60| Type
!width=60| Batch Size
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
! colspan="4" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
! colspan="3" style="color: blue;" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
|rowspan="3"|polyA based isolation NEB UltraII RNAseq
|align="center"|STD
|align="center"| per sample
|align="right"| $157
|align="right"| $173
|align="right"| $236
|Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
|rowspan=2 align="center" style="color: blue;"| HTL (NEB only)
|align="center" style="color: blue;" | ''24''
|align="right" style="color: blue;" | $1,500
|align="right" style="color: blue;" | $1,650
|align="right" style="color: blue;" | $2,100
|Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
|align="center" style="color: blue;" | ''96''
|align="right" style="color: blue;" | $3,000
|align="right" style="color: blue;" | $3,300
|align="right" style="color: blue;" | $4,200
|Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-

|rowspan="2"| High Throughput 3' Digital Gene Expression (HT3DGE)
|rowspan=2 align="center" style="color: blue;"| HTL
|align="center" style="color: blue;"| ''24''
|align="right" style="color: blue;" | $1,050
|align="right" style="color: blue;" | $1,155
|align="right" style="color: blue;" | $1,470
| rowspan="2" style="color: blue;" | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
|align="center" style="color: blue;" | ''96''
|align="right" style="color: blue;" | $2,100
|align="right" style="color: blue;" | $2,310
|align="right" style="color: blue;" | $2,940
|-

|rowspan="3"|Total RNA sequencing NEB RNAseq +Ribodepletion
|align="center"|STD
|align="center"| per sample
|align="right"| $235
|align="right"| $258.5
|align="right"| $352.5
|Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
|rowspan=2 align="center" style="color: blue;"| HTL (NEB only, covaris RNA fragmentation)
|align="center" style="color: blue;" | ''24''
|align="right" style="color: blue;" | $2,500
|align="right" style="color: blue;" | $2,750
|align="right" style="color: blue;" | $3,500
|rowspan=2|Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
|align="center" style="color: blue;" | ''96''
|align="right" style="color: blue;" | $5,000
|align="right" style="color: blue;" | $5,500
|align="right" style="color: blue;" | $7,000
|-

|rowspan=3|Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex
|align="center"| '''STD''' v4
|align="center"|per sample
|align="right"| $262
|align="right"| $289
|align="right"| $394
| rowspan="3" | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
|rowspan="2" align="center" style="color: blue;"| '''HTL''' v2
|align="center" style="color: blue;" | ''24''
|align="right" style="color: blue;" | $2,000
|align="right" style="color: blue;" | $2,200
|align="right" style="color: blue;" | $2,800
|-
|align="center" style="color: blue;" | ''96''
|align="right" style="color: blue;" | $3,200
|align="right" style="color: blue;" | $3,520
|align="right" style="color: blue;" | $4,480
|-

|rowspan="3"|Low Input Mammalian Total Clontech ZapR Kit
|align="center"| STD
|align="center"| per sample
|align="right"| $210
|align="right"| $231
|align="right"| $315
| rowspan="1" | Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
|rowspan=2 align="center" style="color: blue;"| HTL (covaris RNA fragmentation)
|align="center" style="color: blue;" | ''24''
|align="right" style="color: blue;" | $3000
|align="right" style="color: blue;" | $3300
|align="right" style="color: blue;" | $4200
|rowspan=2|Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
|align="center" style="color: blue;" | ''96''
|align="right" style="color: blue;" | $5000
|align="right" style="color: blue;" | $5500
|align="right" style="color: blue;" | $7000
|-
| Small RNA
|align="center"| STD
| align="center"|per sample
| align="right"|$289
| align="right"|$318
| align="right"|$433
| Uses NEB, BIOO or QIAGEN small RNA kit
|-
! colspan="7" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
|colspan=2 style="color: blue;" | High Throughput Setup
|align="center" style="color: blue;" |48 samples
|align="right" style="color: blue;" | $60
|align="right" style="color: blue;" | $66
|align="right" style="color: blue;" | $84
|
|-
|colspan=2|Sample Cleanup
|align="center"|per sample
|align="right"| $5
|align="right"| $5.50
|align="right"| $7
|SPRI cleanup of samples to remove impurities.
|-
|colspan=2 style="color: blue;" |Quantification
|align="center" style="color: blue;" |per sample
|align="right" style="color: blue;" | $10
|align="right" style="color: blue;" | $11
|align="right" style="color: blue;" | $14
|Quantification of samples (FA) prior to arraying.
|-
|}

.

=== LONG READ LIBRARIES ===
{| class="wikitable" border=1
!Oxford Nanopore Sample Preparation
!width=120| Nanopore Kit
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|Standard Ligation || LSK || $160 || $176 || $240
|rowspan=6|Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
|Native Barcoding (ligation indexing) || - || $50 || $55 || $70
|-
|SMARTseq -> Ligation || Cv4+LSK || $300 || $330 || $420
|-
|Rapid Prep (tagment - ~10-20kb) || RAD || $150 || $165 || $225
|-
|Long Insert Prep || RAD (modified, target >100kb) || $262 || $289 || $393
|-
|Direct RNA || RNA004 || $160 || $176 || $240
|}

== SAMPLE EXTRACTION ==
=== CHEMAGIC360 ===
{| class="wikitable" border=1
!TYPE
!width=120| Batch Size
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|DNA
|96
|$700
|$770
|$980
|
|-
|RNA
|96
|$800
|$880
|$1120
|
|}

== SINGLE CELL & SPATIAL SERVICES ==
=== 10X CHROMIUM ===
{| class="wikitable" border=1
! 10X USAGE
!width=120| Batch Size
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|colspan=6 align="center"|'''Assisted Service'''
|-
|colspan=6 |''DIGITAL GENE EXPRESSION''
|-
|rowspan=2 |5' RNA 3' RNA
|setup || $1,015 || $1,116.50 || $1,421 || charge per submission
|-
|per sample|| $1,750 || $1,925 || $2,450 || Includes QC of cDNA and creation of Short Read Library
|-
|rowspan=2 |5' RNA 3' RNA - on chip multiplexing
|setup || $1,150 || $1,265 || $1,1610 || charge per submission
|-
|per 4 samples|| $2,305 || $2,535 || $3,227 || Includes QC of cDNA and creation of Short Read Library
|-
|rowspan=2 |Fixed RNA (Flex v2) probe based. human/mouse only
|setup || $2700 || $2970 || $3780 || charge per submission - upto 48 samples
|-
|per sample || $270|| $297 || $378 || Includes QC of cDNA and creation of Short Read Library
|-
|rowspan=2 |Amplicon (CITE-SEQ, TCR, BCR, antibody barcocde, etc // per amplicon)
|setup || $470 || $517 || $658 || additional prep beyond DGE
|-
|per sample|| $45 || $50 || $63 || Includes QC of cDNA and creation of Short Read Library
|-
|colspan=6 |''scATACseq''
|-
|rowspan=2 |ATACseq
|setup || $880 || $968 || $1,232 || charge per submission
|-
|per sample|| $1,675|| $1,843|| $2,345|| Includes QC and creation of Short Read Library
|-
|colspan=6 |''Multiome (ATAC+3'RNA)''
|-
|rowspan=2 |MULTI
|setup || $1,800|| $1,980|| $2,520|| charge per submission
|-
|per sample|| $3,100|| $3,410|| $4,340|| Includes QC of cDNA and creation of Short Read Library
|-
|colspan=6 |''CANCELLATION''
|-
|per session || || $200|| $220|| $280|| <24h notice
|}
*please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===
{| class="wikitable" border=1
! SLIDES
!width=120| Batch Size
!width=80| KI Only
!width=80| [[BioMicroCenter:CoreDeps|Other CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|Test Array ||slide|| $1,300|| $1,511|| $1,607|| $2435|| recommended 1x per tissue type for fresh frozen
|-
|Sample Slide - Fresh Frozen ||1 slide / 4 tiles|| $6,210|| $6,340|| $6,940|| $9,067 || Includes H&E stain images + Library Generation
|-
|Sample Slide - FFPE (hs/mm only) ||1 slide / 2 tiles|| $4,550|| $4,550|| $4,996|| $6,359 || Includes library generation only.
|-
|Sample Slide - HD (hs/mm only) ||1 slide / 2 tiles|| $7,850|| $7,850|| $8,596|| $10,940 || Includes library generation only.
|}

=== AVITI24 ===
<div style="background:#fff8e6; border-left:4px solid #c8960c; padding:8px 12px; margin-bottom:8px;">'''Beta Service — Early Access:''' Consultation with BMC staff is required before submitting samples. See [[BioMicroCenter:SpTx|Spatial Transcriptomics]] for full service details.</div>
{| class="wikitable" border=1
! AVITI24 CYTOPROFILING / DISS
!width=80|UNIT
!width=80|[[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80|MIT
!width=80|[[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|Atlas Panel (Teton CytoProfiling)
|rowspan=5 align=center| per slide 1-, 12-, 48-well
|align=right| $7,200
|align=right| $7,920
|align=right| $10,080
|RNA + protein + morphology
|-
|Atlas Low (DISS — targeted in situ sequencing)
|align=right| $5,500
|align=right| $6,050
|align=right| $7,700
|User-designed probe; can run simultaneously with Atlas Panel
|-
|Antibody Add-on
|align=right| $360
|align=right| $396
|align=right| $504
|Custom antibody targets; consult BMC staff
|-
|Optimization Run
|align=right| $800
|align=right| $880
|align=right| $1,120
|Charged only if optimization slide fails
|-
|Slide Only (consumable)
|align=right| $150
|align=right| $165
|align=right| $210
|
|}
* High-output DISS (whole transcriptome) not yet released — contact BMC staff.

== OTHER GENOMICS SERVICES ==

 

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===
{| class="wikitable" border=1
! QUALITY CONTROL
!width=100| unit
!width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=100| MIT
!width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|Fragment Analyzer: RNA or DNA
|per sample ||$15 || $16.50 || $21
|
|-
|Fragment Analyzer: Preloaded row
| per row (12) ||$80||$88 || $112
| 2ul per sample in each well using plates from BMC
|-
|FemtoPulse: RNA or DNA
|per sample || $30 || $33 || $42
|
|-
|FemtoPulse: Preloaded Row
|per row (12) || $160 || $176 || $224
| 2ul per sample in each well using plates from BMC
|-
|BioAnalyzer: Walkup Service
|per chip|| $50 || $55 || $70
|
|-
|qPCR only (Short read quantification test)
|per sample || $25 || $27.50 || $35
| 4 point serial dilution
|-
|Full plate qPCR (short read quantification test)
|per plate || $400 || $440 || $560
| full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
|colspan=6 align="center"| Sample QC services:
|-
|Fresh Frozen Curl
|rowspan=2|per curl
| $40|| $44 || $56
|
|-
|FFPE Curl
| $60 || $66 || $84
|
|-
|}

=== OLIGO SYNTHESIS ===
{| class="wikitable" border=1
!Platform
!width=100| Service
!width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=100| MIT
!width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|rowspan=3| Dr.Oligo
|Assisted
|$2000 +consumables
|$2200 +consumables
|$2800 +consumables
|includes setup and run of the instrument only. Large reagents / N2 included.
|-
|Walkup
|$1000 +consumables
|$1100 +consumables
|$1400 +consumables
|includes run of the instrument only. Large reagents / N2 included.
|-
|Training
|$4000
|$4400
|$5600
|ncludes two instrument runs.
|-
|rowspan=6| DNAscript Syntax
|Setup (per run)
|$1000
|$1100
|$1400
|Per run cost
|-
|2000 nucleotide synthesis
|$500
|$550
|$700
|Up to 96 samples
|-
|8000 nucleotide synthesis
|$1500
|$1650
|$2100
|Up to 96 samples
|-
|add Biotin (max48)
|$115
|$126
|$161
|rowspan = 3| max 48 per run and 2 per oligo. Couple to T only.
|-
|add Fluor
|$300
|$330
|$420
|-
|add quench
|$100
|$110
|$140
|}

== [[BioMicroCenter:RTPCR|REAL TIME PCR]] ==

{| border=1
! OTHER LAB SERVICES
!width=100| [[BioMicroCenter:CoreDeps|CORE LAB / MIT]]
!width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=100| unit
! SIGN UP
! Notes
|-
| Roche LightCycler 480
|align="right"| $15
|align="right"| N/A
|align="center"| per plate
| [[BioMicroCenter:Forms#Calenders|CALENDAR]] *available for CORE only
| Contact [[BioMicroCenter:People|Christopher Hallee]] for training
|-
| SYBR Green (KAPA/Roche)
|align="right"| $45
|align="right"| N/A
|align="center"| per ml
|
| Available for MIT only
|-
| 96-well PLATES
|align="right"| $10
|align="right"| N/A
|align="center"| per plate
|
| Available for MIT only
|-
|}
.
 

== OTHER LAB SERVICES ==
{| border=1
! OTHER LAB SERVICES
!width=100| [[BioMicroCenter:CoreDeps|CORE LAB / MIT]]
!width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=100| unit
! SIGN UP
! Notes
|-
|colspan=6 align="center"| '''COVARIS SONICATOR'''
|-
|COVARIS E220
|align="right"|$20
|align="right"|$30
|per hour
|[https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar]
|tubes available for purchase.
|-

| colspan="6" align="center"| '''PIPPIN PREP'''
|-
|Gel Isolation
|align="right"| $75
|align="right"| $105
|align="center"| per gel (5 lanes)
| TBA
|
|-
|Training
|align="right"| $25
|align="right"| $50
|align="center"| per session
| TBA
| Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="6" align="center"| '''PLATE READER'''
|-
| Varioskan
|align="right"| $18
|align="right"| $27
|align="center"| per hour
| [[BioMicroCenter:Forms#Calenders|CALENDAR]]
| Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="6" align="center"| '''ROBOTICS'''
|-
| Tecan EVO 150
|align="right"| $75
|align="right"| $105
|align="center"| per 1h block - based on scheduled time.
| [[BioMicroCenter:Forms#Calenders|CALENDAR]]
|
|-
|non-filter tips
|align="right"| $8
|align="right"| $11.20
|align="center"| per box
|
|** Please let us know if there is additional plasticware you would like us to stock.
|-
|Filter tips
|align="right"| $12.00
|align="right"| $16.80
|align="center"| per box
|
|
|-
| Training
|align="right"| $65
|align="right"| $91
|align="center"| per hour
| Contact [[BioMicroCenter:People|Tatum Murdock]]
|
|-
| colspan="6" align="center"| '''NANODROP'''
|-
| Nanodrop
|align="right"| --
|align="right"| --
|align="center"|
| Walk up service
| * no charge
|-
|}
 

== COMPUTATIONAL SERVICES ==
{| class="wikitable" border=1
! COMPUTATIONAL SERVICES
!width=200| unit
!width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=100| Other MIT / non-MIT
! Notes
|-
| Informatics Support
| align="right"| per hour
| align="right"| $90
| align="right"| $125 / $205
| Very limited support for non-MIT users
|-
| Informatics Share
| align="right"| per 0.1FTE
| align="right"| $1,250
| align="right"| N/A
| MIT only. Priority access to informatics.
|-
|Ingenuity Pathway Analysis
|align="right"|per dataset
|align="right" | $100
|align="right" | $110/$150
| Recovery of licence cost.
|-
|Active Data storage
|align="right"| per TB per year
|align="right"| $100
|align="right"| $300 / N/A
|
|-
| Tape Archive / Recovery
|align="right"| per tape
|align="right"| $60
|align="right"| $70
|
|-
| Informatics Training Classes
|align="right"| per session
|align="right"| $50
|align="right"| $100
|
|}

OLD 
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]] 
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]] 
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]] 
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]] 
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]] 
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]] 
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]] 
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]] 
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]] 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]] 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]] 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]] 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]