BioMicro Center - User contributions [en]

BioMicroCenter:Pricing

2026-04-17T15:41:06Z

Cstjohn: /* 10X CHROMIUM */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| style="text-align:left;" | NovaSeq SP 100nt || $4,623.75 || $5,178.60 || Per flowcell || rowspan="9" style="text-align:left;" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| style="text-align:left;" | NovaSeq SP 300nt** || $6,547.50 || $7,333.20
|-
| style="text-align:left;" | NovaSeq SP 500nt || $8,775.00 || $9,828.00
|-
| style="text-align:left;" | NovaSeq S1 100nt** || $7,290.00 || $8,164.80
|-
| style="text-align:left;" | NovaSeq S1 200nt** || $9,315.00 || $10,432.80
|-
| style="text-align:left;" | NovaSeq S1 300nt** || $10,158.75 || $11,377.80
|-
| style="text-align:left;" | NovaSeq S2 100nt** || $12,993.75 || $14,553.00
|-
| style="text-align:left;" | NovaSeq S2 200nt** || $16,200.00 || $18,144.00
|-
| style="text-align:left;" | NovaSeq S2 300nt** || $16,875.00 || $18,900.00
|-
| style="text-align:left;" | NovaSeq S4 50nt || $4,725.00 || $5,292.00 || Per Lane || rowspan="2" style="text-align:left;" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| style="text-align:left;" | NovaSeq S4 300nt || $6,750.00 || $7,560.00
|-
| style="text-align:left;" | NovaSeqX 10B 150PE Lane^^ || $2,312.50 || $2,590.00 || Per Lane || rowspan="5" style="text-align:left;" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| style="text-align:left;" | NovaSeqX 25B 150PE Lane^^ || $3,250.00 || $3,640.00
|-
| style="text-align:left;" | NovaSeqX 10B 300nt Flowcell^^ || $15,200.00 || $17,024.00 || Per Flowcell
|-
| style="text-align:left;" | NovaSeqX 25B 300nt Flowcell^^ || $20,250.00 || $22,680.00
|-
| style="text-align:left;" | NovaSeqX 25B 100nt Flowcell^^ || $7,187.50 || $8,050.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| rowspan="2" style="vertical-align:middle;" | 75 PE || style="text-align:left;" | Full flowcell (2 lane, ~800M reads) || $2,375.00 || $2,660.00 || per flowcell || rowspan="5" style="text-align:left;" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| style="text-align:left;" | One lane (~400M reads) || $1,250.00 || $1,400.00 || per lane
|-
| rowspan="2" style="vertical-align:middle;" | 150 PE || style="text-align:left;" | Full flowcell (2 lane, ~800M reads) || $3,312.50 || $3,710.00 || per flowcell
|-
| style="text-align:left;" | One lane (~400M reads) || $1,719.00 || $1,925.00 || per lane
|-
| 300 PE || style="text-align:left;" | Full flowcell (~200M reads) || $4,375.00 || $4,900.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| style="text-align:left;" | 50 PE: F3 Lane (~100M reads) || $375.00 || $420.00 || per lane || rowspan="3" style="text-align:left;" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| style="text-align:left;" | Rapid 100nt: F3 flowcell (~400M reads)* || $2,250.00 || $2,520.00 || per flowcell
|-
| style="text-align:left;" | 150 PE: F3 Lane (~100M reads) || $562.50 || $630.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable" style="width:100%; text-align:center;"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
! Length !! Reads !! !! !! !!
|-
| 300nt || 5M || $893.75 || $1,001.00 || rowspan="9" | per flowcell || rowspan="9" style="text-align:left;" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,300.00 || $1,456.00
|-
| 100nt || 25M || $1,156.25 || $1,295.00
|-
| 300nt || 25M || $1,667.50 || $1,867.60
|-
| 600nt || 25M || $1,962.50 || $2,198.00
|-
| 1000nt || 25M || $3,237.50 || $3,626.00
|-
| 100nt || 100M || $1,925.00 || $2,156.00
|-
| 300nt || 100M || $2,656.00 || $2,975.00
|-
| 600nt || 50M || $2,656.00 || $2,975.00
|}

==== MiSeq i100 Walkup ====

Walkup service is not available to external users.

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable" style="width:100%; text-align:center;"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| style="text-align:left;" | 70nt V2 kit (eg. 30PE or 70SE) || $1,650.00 || $1,848.00 || rowspan="6" | per flowcell || rowspan="6" style="text-align:left;" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| style="text-align:left;" | 150nt V3 kits (eg. 75PE) || $1,787.50 || $2,002.00
|-
| style="text-align:left;" | 300nt V2 kit (eg. 150PE) || $1,993.75 || $2,233.00
|-
| style="text-align:left;" | 500nt V2 kit (eg. 250PE) || $2,200.00 || $2,464.00
|-
| style="text-align:left;" | 600nt V3 kit (eg. 300PE) || $2,750.00 || $3,080.00
|-
| style="text-align:left;" | 500nt NANO Run (eg. 250PE) || $962.50 || $1,078.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| style="text-align:left;" | Quality Control Only - only one QC is included per sequencing lane. || $43.75 || $49.00 || per sample || style="text-align:left;" | Includes RT-PCR and Frag.Analyzer
|-
| style="text-align:left;" | Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $18.75 || $21.00 || per sample || style="text-align:left;" | Includes Fluorometric quantification and Frag.Analyzer
|-
| style="text-align:left;" | qPCR only (short read) || $31.25 || $35.00 || per sample || style="text-align:left;" | Includes qPCR values from standard QC only
|-
| style="text-align:left;" | qPCR only - full plate (short read) || $500.00 || $560.00 || per plate || style="text-align:left;" | excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable" style="width:100%; text-align:center;"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| style="text-align:left;" | 72 hour Flowcell || $1,500.00 || $1,680.00 || per Flowcell || style="text-align:left;" | Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable" style="width:100%; text-align:center;"
|-

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Illumina || Singular || style="text-align:left;" | Index Overwrite (per sample) || $37.50 || $42.00 || style="text-align:left;" | Includes QC and reindexing
|-
| Illumina || Singular || style="text-align:left;" | P5/P7 conversion (per pool) || $125.00 || $140.00 || style="text-align:left;" | Includes QC. Samples not reindexed. Requires full flowcell for sequencing (Rapid)
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="3" style="text-align:left;" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. || colspan="3" style="text-align:center;" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| rowspan="3" style="vertical-align:middle; text-align:left;" | LIGATION BASED NEB UltraII || STD || per sample || $136.50 || $157.00 || style="text-align:left;" | Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| HTL || 24 || $1,125.00 || $1,260.00 || style="text-align:left;" | Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| HTL || 96 || $2,250.00 || $2,520.00
|-
| style="text-align:left;" | Tagmentation NEXTERA-XT || STD || per Sample || $136.50 || $157.00 || rowspan="2" style="text-align:left;" | Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| style="text-align:left;" | Tagmentation NEXTERA-FLEX || STD || per Sample || $204.00 || $235.00
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | NEXTERA-XT OR FLEX || HTL || 24 || $1,125.00 || $1,260.00 || style="text-align:left;" | Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| HTL || 96 || $2,250.00 || $2,520.00
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | 16S Amplicon || HTL || 48 || $2,375.00 || $2,660.00 || style="text-align:left;" | Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || add'l 48 || $812.50 || $910.00
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | Other Amplicon || HTL || 48 || $2,000.00 || $2,240.00 || style="text-align:left;" | Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || add'l 48 || N/A || $945.00
|-
| colspan="6" style="text-align:center;" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| style="text-align:left;" | High Throughput Setup || HTL || 48 samples || $75.00 || $84.00
|-
| style="text-align:left;" | Sample Cleanup || HTL || per sample || $6.25 || $7.00 || style="text-align:left;" | SPRI cleanup of samples to remove impurities.
|-
| style="text-align:left;" | Quantification || HTL || per sample || $12.50 || $14.00 || style="text-align:left;" | Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="3" style="text-align:left;" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. || colspan="3" style="text-align:center;" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| rowspan="3" style="vertical-align:middle; text-align:left;" | polyA based isolation NEB UltraII RNAseq || STD || per sample || $196.25 || $219.80 || style="text-align:left;" | Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| HTL (NEB only) || 24 || $1,875.00 || $2,100.00 || style="text-align:left;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| HTL (NEB only) || 96 || $3,750.00 || $4,200.00 ||
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | High Throughput 3' Digital Gene Expression (HT3DGE) || HTL || 24 || $1,312.50 || $1,470.00 || style="text-align:left;" | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| HTL || 96 || $2,625.00 || $2,940.00 ||
|-
| rowspan="3" style="vertical-align:middle; text-align:left;" | Total RNA sequencing NEB RNAseq +Ribodepletion || STD || per sample || $293.75 || $329.00 || style="text-align:left;" | Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| HTL (NEB only, covaris RNA fragmentation) || 24 || $3,125.00 || $3,500.00 || style="text-align:left;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || 96 || $6,250.00 || $7,000.00 ||
|-
| rowspan="3" style="vertical-align:middle; text-align:left;" | Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || STD v4 || per sample || $340.60 || $394.00 || style="text-align:left;" | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| HTL v2 || 24 || $2,500.00 || $2,800.00 ||
|-
| || 96 || $4,000.00 || $4,480.00 ||
|-
| rowspan="3" style="vertical-align:middle; text-align:left;" | Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $273.00 || $315.00 || style="text-align:left;" | Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| HTL (covaris RNA fragmentation) || 24 || $3,750.00 || $4,200.00 || style="text-align:left;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || 96 || $6,250.00 || $7,000.00 ||
|-
| style="text-align:left;" | Small RNA || STD || per sample || $375.70 || $433.00 || style="text-align:left;" | Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" style="text-align:center;" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| style="text-align:left;" | High Throughput Setup || HTL || 48 samples || $75.00 || $84.00
|-
| style="text-align:left;" | Sample Cleanup || HTL || per sample || $6.25 || $7.00 || style="text-align:left;" | SPRI cleanup of samples to remove impurities.
|-
| style="text-align:left;" | Quantification || HTL || per sample || $12.50 || $14.00 || style="text-align:left;" | Quantification of samples (FA) prior to arraying.
|}

=== LONG READ LIBRARIES ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| style="text-align:left;" | Standard Ligation || LSK || $200.00 || $240.00 || rowspan="6" style="text-align:left;" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| style="text-align:left;" | Native Barcoding (ligation indexing) || - || $65.00 || $70.00
|-
| style="text-align:left;" | SMARTseq -> Ligation || Cv4+LSK || $375.00 || $450.00
|-
| style="text-align:left;" | Rapid Prep (tagment - ~10-20kb) || RAD || $188.00 || $225.00
|-
| style="text-align:left;" | Long Insert Prep || RAD (modified, target >100kb) || $340.60 || $393.00
|-
| style="text-align:left;" | Direct RNA || RNA004 || $200.00 || $240.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| DNA || 96 || $875.00 || $980.00 ||
|-
| RNA || 96 || $1,000.00 || $1,120.00 ||
|-
| style="text-align:left;" | RNA/DNA Isolation - Assisted || 12 samples || $375.00 || $420.00 ||
|-
| style="text-align:left;" | RNA/DNA Isolation - Assisted || 96 samples || $1,250.00 || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | 5' RNA / 3' RNA || setup || $1,268.75 || $1,421.00 || style="text-align:left;" | charge per submission
|-
| per sample || $2,188.00 || $2,450.00 || style="text-align:left;" | Includes QC of cDNA and creation of Short Read Library
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | 5' RNA / 3' RNA - on chip multiplexing || setup || $1,437.50 || $1,610.00 || style="text-align:left;" | charge per submission
|-
| per 4 samples || $2,881.00 || $3,227.00 || style="text-align:left;" | Includes QC of cDNA and creation of Short Read Library
|-
| rowspan="3" style="vertical-align:middle; text-align:left;" | Fixed RNA (Flex v2) probe based. human/mouse only || setup (up to 48 samples) || $3,375.00 || $3,780.00 || style="text-align:left;" | charge per submission
|-
| per sample (1-plex) || $337.50 || $378.00 || style="text-align:left;" | Includes QC of cDNA and creation of Short Read Library
|-
| per 4-plex || $4,875.00 || $5,460.00 ||
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) || setup || $587.50 || $658.00 || style="text-align:left;" | additional prep beyond DGE
|-
| per sample || $56.00 || $63.00 || style="text-align:left;" | Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" style="text-align:left;" | '''scATACseq'''
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | ATACseq || setup || $1,100.00 || $1,232.00 || style="text-align:left;" | charge per submission
|-
| per sample || $2,094.00 || $2,345.00 || style="text-align:left;" | Includes QC and creation of Short Read Library
|-
| colspan="5" style="text-align:left;" | '''Multiome (ATAC+3'RNA)'''
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | MULTI || setup || $2,250.00 || $2,520.00 || style="text-align:left;" | charge per submission
|-
| per sample || $3,875.00 || $4,340.00 || style="text-align:left;" | Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" style="text-align:left;" | '''Cancellation'''
|-
| style="text-align:left;" | per session || <24h notice || $250.00 || $280.00 ||
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| style="text-align:left;" | Test Array || slide || $1,217.00 || $1,363.00 || style="text-align:left;" | recommended 1x per tissue type for fresh frozen
|-
| style="text-align:left;" | Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $7,510.00 || $8,411.00 || style="text-align:left;" | Includes H&E stain images + Library Generation
|-
| style="text-align:left;" | Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $5,688.00 || $6,359.00 || style="text-align:left;" | Includes library generation only.
|-
| style="text-align:left;" | Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $9,813.00 || $10,940.00 || style="text-align:left;" | Includes library generation only.
|-
| style="text-align:left;" | QC - FFPE Scroll || per scroll || $75.00 || $84.00 || style="text-align:left;" | RNA isolation from FFPE scroll
|-
| style="text-align:left;" | QC - Fresh Frozen Scroll || per scroll || $60.00 || $66.00 || style="text-align:left;" | RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| style="text-align:left;" | Slide Only || per slide || $188.00 || $210.00 ||
|-
| style="text-align:left;" | Optimization Only || per slide || $1,000.00 || $1,120.00 ||
|-
| style="text-align:left;" | Teton Panel || per slide || $9,000.00 || $10,080.00 ||
|-
| style="text-align:left;" | Atlas Short || per slide || $6,875.00 || $7,700.00 ||
|-
| style="text-align:left;" | Antibody Add-on || per slide || $450.00 || $504.00 ||
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| style="text-align:left;" | Fragment Analyzer: RNA or DNA || per sample || $18.75 || $21.00 ||
|-
| style="text-align:left;" | Fragment Analyzer: Preloaded row || per row (12) || $100.00 || $112.00 || style="text-align:left;" | 2ul per sample in each well using plates from BMC
|-
| style="text-align:left;" | FemtoPulse: RNA or DNA || per sample || $37.50 || $42.00 ||
|-
| style="text-align:left;" | FemtoPulse: Preloaded Row || per row (12) || $200.00 || $224.00 || style="text-align:left;" | 2ul per sample in each well using plates from BMC
|-
| style="text-align:left;" | BioAnalyzer: Walkup Service || per chip || $62.50 || $70.00 ||
|-
| style="text-align:left;" | qPCR only (Short read quantification test) || per sample || $31.25 || $35.00 || style="text-align:left;" | 4 point serial dilution
|-
| style="text-align:left;" | Full plate qPCR (short read quantification test) || per plate || $500.00 || $560.00 || style="text-align:left;" | full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" style="text-align:left;" | '''Sample QC services:'''
|-
| style="text-align:left;" | Fresh Frozen Curl || per curl || $60.00 || $66.00 ||
|-
| style="text-align:left;" | FFPE Curl || per curl || $75.00 || $84.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| rowspan="3" style="vertical-align:middle;" | Dr.Oligo || style="text-align:left;" | Assisted || $2,500.00 +consumables || $2,800.00 +consumables || style="text-align:left;" | includes setup and run of the instrument only. Large reagents / N2 included.
|-
| style="text-align:left;" | Walkup || $1,250.00 +consumables || $1,400.00 +consumables || style="text-align:left;" | includes run of the instrument only. Large reagents / N2 included.
|-
| style="text-align:left;" | Training || $5,000.00 || $5,600.00 || style="text-align:left;" | includes two instrument runs.
|-
| rowspan="6" style="vertical-align:middle;" | DNAscript Syntax || style="text-align:left;" | Setup (per run) || $1,250.00 || $1,400.00 || style="text-align:left;" | Per run cost
|-
| style="text-align:left;" | 2000 nucleotide synthesis || $625.00 || $700.00 || style="text-align:left;" | Up to 96 samples
|-
| style="text-align:left;" | 8000 nucleotide synthesis || $1,875.00 || $2,100.00 || style="text-align:left;" | Up to 96 samples
|-
| style="text-align:left;" | add Biotin (max 48) || $144.00 || $161.00 || style="text-align:left;" | max 48 per run and 2 per oligo. Couple to T only.
|-
| style="text-align:left;" | add Fluor || $375.00 || $420.00 ||
|-
| style="text-align:left;" | add Quench || $125.00 || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to external users.

== OTHER LAB SERVICES ==

{| class="wikitable" style="width:100%; text-align:center;"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! SIGN UP !! Notes
|-
| colspan="6" style="text-align:left;" | '''COVARIS SONICATOR'''
|-
| style="text-align:left;" | COVARIS E220 || $25.00 || $28.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || style="text-align:left;" | tubes available for purchase.
|-
| colspan="6" style="text-align:left;" | '''PIPPIN PREP'''
|-
| style="text-align:left;" | Gel Isolation || $93.75 || $105.00 || per gel (5 lanes) || TBA ||
|-
| colspan="6" style="text-align:left;" | '''PLATE READER'''
|-
| style="text-align:left;" | Varioskan || $23.00 || $27.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || style="text-align:left;" | Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="6" style="text-align:left;" | '''ROBOTICS'''
|-
| style="text-align:left;" | Tecan EVO 150 || $93.75 || $105.00 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| style="text-align:left;" | non-filter tips || $10.00 || $11.20 || per box || || style="text-align:left;" | ** Please let us know if there is additional plasticware you would like us to stock.
|-
| style="text-align:left;" | Filter tips || $15.00 || $16.80 || per box || ||
|-
| style="text-align:left;" | Training || $81.25 || $91.00 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]] ||
|-
| colspan="6" style="text-align:left;" | '''NANODROP'''
|-
| style="text-align:left;" | Nanodrop || -- || -- || || Walk up service || style="text-align:left;" | * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable" style="width:100%; text-align:center;"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| style="text-align:left;" | Informatics Support || per hour || $180.00 || $205.00 || style="text-align:left;" | Very limited support for external users
|-
| style="text-align:left;" | Ingenuity Pathway Analysis || per dataset || N/A || $150.00 || style="text-align:left;" | Recovery of licence cost.
|}

BioMicroCenter:Pricing

2026-04-17T15:40:34Z

Cstjohn: /* SHORT READ LIBRARY - RNA */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| style="text-align:left;" | NovaSeq SP 100nt || $4,623.75 || $5,178.60 || Per flowcell || rowspan="9" style="text-align:left;" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| style="text-align:left;" | NovaSeq SP 300nt** || $6,547.50 || $7,333.20
|-
| style="text-align:left;" | NovaSeq SP 500nt || $8,775.00 || $9,828.00
|-
| style="text-align:left;" | NovaSeq S1 100nt** || $7,290.00 || $8,164.80
|-
| style="text-align:left;" | NovaSeq S1 200nt** || $9,315.00 || $10,432.80
|-
| style="text-align:left;" | NovaSeq S1 300nt** || $10,158.75 || $11,377.80
|-
| style="text-align:left;" | NovaSeq S2 100nt** || $12,993.75 || $14,553.00
|-
| style="text-align:left;" | NovaSeq S2 200nt** || $16,200.00 || $18,144.00
|-
| style="text-align:left;" | NovaSeq S2 300nt** || $16,875.00 || $18,900.00
|-
| style="text-align:left;" | NovaSeq S4 50nt || $4,725.00 || $5,292.00 || Per Lane || rowspan="2" style="text-align:left;" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| style="text-align:left;" | NovaSeq S4 300nt || $6,750.00 || $7,560.00
|-
| style="text-align:left;" | NovaSeqX 10B 150PE Lane^^ || $2,312.50 || $2,590.00 || Per Lane || rowspan="5" style="text-align:left;" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| style="text-align:left;" | NovaSeqX 25B 150PE Lane^^ || $3,250.00 || $3,640.00
|-
| style="text-align:left;" | NovaSeqX 10B 300nt Flowcell^^ || $15,200.00 || $17,024.00 || Per Flowcell
|-
| style="text-align:left;" | NovaSeqX 25B 300nt Flowcell^^ || $20,250.00 || $22,680.00
|-
| style="text-align:left;" | NovaSeqX 25B 100nt Flowcell^^ || $7,187.50 || $8,050.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| rowspan="2" style="vertical-align:middle;" | 75 PE || style="text-align:left;" | Full flowcell (2 lane, ~800M reads) || $2,375.00 || $2,660.00 || per flowcell || rowspan="5" style="text-align:left;" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| style="text-align:left;" | One lane (~400M reads) || $1,250.00 || $1,400.00 || per lane
|-
| rowspan="2" style="vertical-align:middle;" | 150 PE || style="text-align:left;" | Full flowcell (2 lane, ~800M reads) || $3,312.50 || $3,710.00 || per flowcell
|-
| style="text-align:left;" | One lane (~400M reads) || $1,719.00 || $1,925.00 || per lane
|-
| 300 PE || style="text-align:left;" | Full flowcell (~200M reads) || $4,375.00 || $4,900.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| style="text-align:left;" | 50 PE: F3 Lane (~100M reads) || $375.00 || $420.00 || per lane || rowspan="3" style="text-align:left;" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| style="text-align:left;" | Rapid 100nt: F3 flowcell (~400M reads)* || $2,250.00 || $2,520.00 || per flowcell
|-
| style="text-align:left;" | 150 PE: F3 Lane (~100M reads) || $562.50 || $630.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable" style="width:100%; text-align:center;"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
! Length !! Reads !! !! !! !!
|-
| 300nt || 5M || $893.75 || $1,001.00 || rowspan="9" | per flowcell || rowspan="9" style="text-align:left;" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,300.00 || $1,456.00
|-
| 100nt || 25M || $1,156.25 || $1,295.00
|-
| 300nt || 25M || $1,667.50 || $1,867.60
|-
| 600nt || 25M || $1,962.50 || $2,198.00
|-
| 1000nt || 25M || $3,237.50 || $3,626.00
|-
| 100nt || 100M || $1,925.00 || $2,156.00
|-
| 300nt || 100M || $2,656.00 || $2,975.00
|-
| 600nt || 50M || $2,656.00 || $2,975.00
|}

==== MiSeq i100 Walkup ====

Walkup service is not available to external users.

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable" style="width:100%; text-align:center;"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| style="text-align:left;" | 70nt V2 kit (eg. 30PE or 70SE) || $1,650.00 || $1,848.00 || rowspan="6" | per flowcell || rowspan="6" style="text-align:left;" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| style="text-align:left;" | 150nt V3 kits (eg. 75PE) || $1,787.50 || $2,002.00
|-
| style="text-align:left;" | 300nt V2 kit (eg. 150PE) || $1,993.75 || $2,233.00
|-
| style="text-align:left;" | 500nt V2 kit (eg. 250PE) || $2,200.00 || $2,464.00
|-
| style="text-align:left;" | 600nt V3 kit (eg. 300PE) || $2,750.00 || $3,080.00
|-
| style="text-align:left;" | 500nt NANO Run (eg. 250PE) || $962.50 || $1,078.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| style="text-align:left;" | Quality Control Only - only one QC is included per sequencing lane. || $43.75 || $49.00 || per sample || style="text-align:left;" | Includes RT-PCR and Frag.Analyzer
|-
| style="text-align:left;" | Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $18.75 || $21.00 || per sample || style="text-align:left;" | Includes Fluorometric quantification and Frag.Analyzer
|-
| style="text-align:left;" | qPCR only (short read) || $31.25 || $35.00 || per sample || style="text-align:left;" | Includes qPCR values from standard QC only
|-
| style="text-align:left;" | qPCR only - full plate (short read) || $500.00 || $560.00 || per plate || style="text-align:left;" | excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable" style="width:100%; text-align:center;"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| style="text-align:left;" | 72 hour Flowcell || $1,500.00 || $1,680.00 || per Flowcell || style="text-align:left;" | Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable" style="width:100%; text-align:center;"
|-

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Illumina || Singular || style="text-align:left;" | Index Overwrite (per sample) || $37.50 || $42.00 || style="text-align:left;" | Includes QC and reindexing
|-
| Illumina || Singular || style="text-align:left;" | P5/P7 conversion (per pool) || $125.00 || $140.00 || style="text-align:left;" | Includes QC. Samples not reindexed. Requires full flowcell for sequencing (Rapid)
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="3" style="text-align:left;" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. || colspan="3" style="text-align:center;" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| rowspan="3" style="vertical-align:middle; text-align:left;" | LIGATION BASED NEB UltraII || STD || per sample || $136.50 || $157.00 || style="text-align:left;" | Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| HTL || 24 || $1,125.00 || $1,260.00 || style="text-align:left;" | Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| HTL || 96 || $2,250.00 || $2,520.00
|-
| style="text-align:left;" | Tagmentation NEXTERA-XT || STD || per Sample || $136.50 || $157.00 || rowspan="2" style="text-align:left;" | Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| style="text-align:left;" | Tagmentation NEXTERA-FLEX || STD || per Sample || $204.00 || $235.00
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | NEXTERA-XT OR FLEX || HTL || 24 || $1,125.00 || $1,260.00 || style="text-align:left;" | Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| HTL || 96 || $2,250.00 || $2,520.00
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | 16S Amplicon || HTL || 48 || $2,375.00 || $2,660.00 || style="text-align:left;" | Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || add'l 48 || $812.50 || $910.00
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | Other Amplicon || HTL || 48 || $2,000.00 || $2,240.00 || style="text-align:left;" | Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || add'l 48 || N/A || $945.00
|-
| colspan="6" style="text-align:center;" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| style="text-align:left;" | High Throughput Setup || HTL || 48 samples || $75.00 || $84.00
|-
| style="text-align:left;" | Sample Cleanup || HTL || per sample || $6.25 || $7.00 || style="text-align:left;" | SPRI cleanup of samples to remove impurities.
|-
| style="text-align:left;" | Quantification || HTL || per sample || $12.50 || $14.00 || style="text-align:left;" | Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="3" style="text-align:left;" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. || colspan="3" style="text-align:center;" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| rowspan="3" style="vertical-align:middle; text-align:left;" | polyA based isolation NEB UltraII RNAseq || STD || per sample || $196.25 || $219.80 || style="text-align:left;" | Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| HTL (NEB only) || 24 || $1,875.00 || $2,100.00 || style="text-align:left;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| HTL (NEB only) || 96 || $3,750.00 || $4,200.00 ||
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | High Throughput 3' Digital Gene Expression (HT3DGE) || HTL || 24 || $1,312.50 || $1,470.00 || style="text-align:left;" | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| HTL || 96 || $2,625.00 || $2,940.00 ||
|-
| rowspan="3" style="vertical-align:middle; text-align:left;" | Total RNA sequencing NEB RNAseq +Ribodepletion || STD || per sample || $293.75 || $329.00 || style="text-align:left;" | Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| HTL (NEB only, covaris RNA fragmentation) || 24 || $3,125.00 || $3,500.00 || style="text-align:left;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || 96 || $6,250.00 || $7,000.00 ||
|-
| rowspan="3" style="vertical-align:middle; text-align:left;" | Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || STD v4 || per sample || $340.60 || $394.00 || style="text-align:left;" | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| HTL v2 || 24 || $2,500.00 || $2,800.00 ||
|-
| || 96 || $4,000.00 || $4,480.00 ||
|-
| rowspan="3" style="vertical-align:middle; text-align:left;" | Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $273.00 || $315.00 || style="text-align:left;" | Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| HTL (covaris RNA fragmentation) || 24 || $3,750.00 || $4,200.00 || style="text-align:left;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || 96 || $6,250.00 || $7,000.00 ||
|-
| style="text-align:left;" | Small RNA || STD || per sample || $375.70 || $433.00 || style="text-align:left;" | Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" style="text-align:center;" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| style="text-align:left;" | High Throughput Setup || HTL || 48 samples || $75.00 || $84.00
|-
| style="text-align:left;" | Sample Cleanup || HTL || per sample || $6.25 || $7.00 || style="text-align:left;" | SPRI cleanup of samples to remove impurities.
|-
| style="text-align:left;" | Quantification || HTL || per sample || $12.50 || $14.00 || style="text-align:left;" | Quantification of samples (FA) prior to arraying.
|}

=== LONG READ LIBRARIES ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| style="text-align:left;" | Standard Ligation || LSK || $200.00 || $240.00 || rowspan="6" style="text-align:left;" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| style="text-align:left;" | Native Barcoding (ligation indexing) || - || $65.00 || $70.00
|-
| style="text-align:left;" | SMARTseq -> Ligation || Cv4+LSK || $375.00 || $450.00
|-
| style="text-align:left;" | Rapid Prep (tagment - ~10-20kb) || RAD || $188.00 || $225.00
|-
| style="text-align:left;" | Long Insert Prep || RAD (modified, target >100kb) || $340.60 || $393.00
|-
| style="text-align:left;" | Direct RNA || RNA004 || $200.00 || $240.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| DNA || 96 || $875.00 || $980.00 ||
|-
| RNA || 96 || $1,000.00 || $1,120.00 ||
|-
| style="text-align:left;" | RNA/DNA Isolation - Assisted || 12 samples || $375.00 || $420.00 ||
|-
| style="text-align:left;" | RNA/DNA Isolation - Assisted || 96 samples || $1,250.00 || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="5" style="text-align:left;" | '''Assisted Service'''
|-
| colspan="5" style="text-align:left;" | '''Digital Gene Expression'''
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | 5' RNA / 3' RNA || setup || $1,268.75 || $1,421.00 || style="text-align:left;" | charge per submission
|-
| per sample || $2,188.00 || $2,450.00 || style="text-align:left;" | Includes QC of cDNA and creation of Short Read Library
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | 5' RNA / 3' RNA - on chip multiplexing || setup || $1,437.50 || $1,610.00 || style="text-align:left;" | charge per submission
|-
| per 4 samples || $2,881.00 || $3,227.00 || style="text-align:left;" | Includes QC of cDNA and creation of Short Read Library
|-
| rowspan="3" style="vertical-align:middle; text-align:left;" | Fixed RNA (Flex v2) probe based. human/mouse only || setup (up to 48 samples) || $3,375.00 || $3,780.00 || style="text-align:left;" | charge per submission
|-
| per sample (1-plex) || $337.50 || $378.00 || style="text-align:left;" | Includes QC of cDNA and creation of Short Read Library
|-
| per 4-plex || $4,875.00 || $5,460.00 ||
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) || setup || $587.50 || $658.00 || style="text-align:left;" | additional prep beyond DGE
|-
| per sample || $56.00 || $63.00 || style="text-align:left;" | Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" style="text-align:left;" | '''scATACseq'''
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | ATACseq || setup || $1,100.00 || $1,232.00 || style="text-align:left;" | charge per submission
|-
| per sample || $2,094.00 || $2,345.00 || style="text-align:left;" | Includes QC and creation of Short Read Library
|-
| colspan="5" style="text-align:left;" | '''Multiome (ATAC+3'RNA)'''
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | MULTI || setup || $2,250.00 || $2,520.00 || style="text-align:left;" | charge per submission
|-
| per sample || $3,875.00 || $4,340.00 || style="text-align:left;" | Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" style="text-align:left;" | '''Cancellation'''
|-
| style="text-align:left;" | per session || <24h notice || $250.00 || $280.00 ||
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| style="text-align:left;" | Test Array || slide || $1,217.00 || $1,363.00 || style="text-align:left;" | recommended 1x per tissue type for fresh frozen
|-
| style="text-align:left;" | Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $7,510.00 || $8,411.00 || style="text-align:left;" | Includes H&E stain images + Library Generation
|-
| style="text-align:left;" | Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $5,688.00 || $6,359.00 || style="text-align:left;" | Includes library generation only.
|-
| style="text-align:left;" | Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $9,813.00 || $10,940.00 || style="text-align:left;" | Includes library generation only.
|-
| style="text-align:left;" | QC - FFPE Scroll || per scroll || $75.00 || $84.00 || style="text-align:left;" | RNA isolation from FFPE scroll
|-
| style="text-align:left;" | QC - Fresh Frozen Scroll || per scroll || $60.00 || $66.00 || style="text-align:left;" | RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| style="text-align:left;" | Slide Only || per slide || $188.00 || $210.00 ||
|-
| style="text-align:left;" | Optimization Only || per slide || $1,000.00 || $1,120.00 ||
|-
| style="text-align:left;" | Teton Panel || per slide || $9,000.00 || $10,080.00 ||
|-
| style="text-align:left;" | Atlas Short || per slide || $6,875.00 || $7,700.00 ||
|-
| style="text-align:left;" | Antibody Add-on || per slide || $450.00 || $504.00 ||
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| style="text-align:left;" | Fragment Analyzer: RNA or DNA || per sample || $18.75 || $21.00 ||
|-
| style="text-align:left;" | Fragment Analyzer: Preloaded row || per row (12) || $100.00 || $112.00 || style="text-align:left;" | 2ul per sample in each well using plates from BMC
|-
| style="text-align:left;" | FemtoPulse: RNA or DNA || per sample || $37.50 || $42.00 ||
|-
| style="text-align:left;" | FemtoPulse: Preloaded Row || per row (12) || $200.00 || $224.00 || style="text-align:left;" | 2ul per sample in each well using plates from BMC
|-
| style="text-align:left;" | BioAnalyzer: Walkup Service || per chip || $62.50 || $70.00 ||
|-
| style="text-align:left;" | qPCR only (Short read quantification test) || per sample || $31.25 || $35.00 || style="text-align:left;" | 4 point serial dilution
|-
| style="text-align:left;" | Full plate qPCR (short read quantification test) || per plate || $500.00 || $560.00 || style="text-align:left;" | full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" style="text-align:left;" | '''Sample QC services:'''
|-
| style="text-align:left;" | Fresh Frozen Curl || per curl || $60.00 || $66.00 ||
|-
| style="text-align:left;" | FFPE Curl || per curl || $75.00 || $84.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| rowspan="3" style="vertical-align:middle;" | Dr.Oligo || style="text-align:left;" | Assisted || $2,500.00 +consumables || $2,800.00 +consumables || style="text-align:left;" | includes setup and run of the instrument only. Large reagents / N2 included.
|-
| style="text-align:left;" | Walkup || $1,250.00 +consumables || $1,400.00 +consumables || style="text-align:left;" | includes run of the instrument only. Large reagents / N2 included.
|-
| style="text-align:left;" | Training || $5,000.00 || $5,600.00 || style="text-align:left;" | includes two instrument runs.
|-
| rowspan="6" style="vertical-align:middle;" | DNAscript Syntax || style="text-align:left;" | Setup (per run) || $1,250.00 || $1,400.00 || style="text-align:left;" | Per run cost
|-
| style="text-align:left;" | 2000 nucleotide synthesis || $625.00 || $700.00 || style="text-align:left;" | Up to 96 samples
|-
| style="text-align:left;" | 8000 nucleotide synthesis || $1,875.00 || $2,100.00 || style="text-align:left;" | Up to 96 samples
|-
| style="text-align:left;" | add Biotin (max 48) || $144.00 || $161.00 || style="text-align:left;" | max 48 per run and 2 per oligo. Couple to T only.
|-
| style="text-align:left;" | add Fluor || $375.00 || $420.00 ||
|-
| style="text-align:left;" | add Quench || $125.00 || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to external users.

== OTHER LAB SERVICES ==

{| class="wikitable" style="width:100%; text-align:center;"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! SIGN UP !! Notes
|-
| colspan="6" style="text-align:left;" | '''COVARIS SONICATOR'''
|-
| style="text-align:left;" | COVARIS E220 || $25.00 || $28.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || style="text-align:left;" | tubes available for purchase.
|-
| colspan="6" style="text-align:left;" | '''PIPPIN PREP'''
|-
| style="text-align:left;" | Gel Isolation || $93.75 || $105.00 || per gel (5 lanes) || TBA ||
|-
| colspan="6" style="text-align:left;" | '''PLATE READER'''
|-
| style="text-align:left;" | Varioskan || $23.00 || $27.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || style="text-align:left;" | Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="6" style="text-align:left;" | '''ROBOTICS'''
|-
| style="text-align:left;" | Tecan EVO 150 || $93.75 || $105.00 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| style="text-align:left;" | non-filter tips || $10.00 || $11.20 || per box || || style="text-align:left;" | ** Please let us know if there is additional plasticware you would like us to stock.
|-
| style="text-align:left;" | Filter tips || $15.00 || $16.80 || per box || ||
|-
| style="text-align:left;" | Training || $81.25 || $91.00 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]] ||
|-
| colspan="6" style="text-align:left;" | '''NANODROP'''
|-
| style="text-align:left;" | Nanodrop || -- || -- || || Walk up service || style="text-align:left;" | * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable" style="width:100%; text-align:center;"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| style="text-align:left;" | Informatics Support || per hour || $180.00 || $205.00 || style="text-align:left;" | Very limited support for external users
|-
| style="text-align:left;" | Ingenuity Pathway Analysis || per dataset || N/A || $150.00 || style="text-align:left;" | Recovery of licence cost.
|}

BioMicroCenter:Pricing

2026-04-17T15:31:53Z

Cstjohn: /* SHORT READ LIBRARY - DNA */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| style="text-align:left;" | NovaSeq SP 100nt || $4,623.75 || $5,178.60 || Per flowcell || rowspan="9" style="text-align:left;" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| style="text-align:left;" | NovaSeq SP 300nt** || $6,547.50 || $7,333.20
|-
| style="text-align:left;" | NovaSeq SP 500nt || $8,775.00 || $9,828.00
|-
| style="text-align:left;" | NovaSeq S1 100nt** || $7,290.00 || $8,164.80
|-
| style="text-align:left;" | NovaSeq S1 200nt** || $9,315.00 || $10,432.80
|-
| style="text-align:left;" | NovaSeq S1 300nt** || $10,158.75 || $11,377.80
|-
| style="text-align:left;" | NovaSeq S2 100nt** || $12,993.75 || $14,553.00
|-
| style="text-align:left;" | NovaSeq S2 200nt** || $16,200.00 || $18,144.00
|-
| style="text-align:left;" | NovaSeq S2 300nt** || $16,875.00 || $18,900.00
|-
| style="text-align:left;" | NovaSeq S4 50nt || $4,725.00 || $5,292.00 || Per Lane || rowspan="2" style="text-align:left;" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| style="text-align:left;" | NovaSeq S4 300nt || $6,750.00 || $7,560.00
|-
| style="text-align:left;" | NovaSeqX 10B 150PE Lane^^ || $2,312.50 || $2,590.00 || Per Lane || rowspan="5" style="text-align:left;" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| style="text-align:left;" | NovaSeqX 25B 150PE Lane^^ || $3,250.00 || $3,640.00
|-
| style="text-align:left;" | NovaSeqX 10B 300nt Flowcell^^ || $15,200.00 || $17,024.00 || Per Flowcell
|-
| style="text-align:left;" | NovaSeqX 25B 300nt Flowcell^^ || $20,250.00 || $22,680.00
|-
| style="text-align:left;" | NovaSeqX 25B 100nt Flowcell^^ || $7,187.50 || $8,050.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| rowspan="2" style="vertical-align:middle;" | 75 PE || style="text-align:left;" | Full flowcell (2 lane, ~800M reads) || $2,375.00 || $2,660.00 || per flowcell || rowspan="5" style="text-align:left;" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| style="text-align:left;" | One lane (~400M reads) || $1,250.00 || $1,400.00 || per lane
|-
| rowspan="2" style="vertical-align:middle;" | 150 PE || style="text-align:left;" | Full flowcell (2 lane, ~800M reads) || $3,312.50 || $3,710.00 || per flowcell
|-
| style="text-align:left;" | One lane (~400M reads) || $1,719.00 || $1,925.00 || per lane
|-
| 300 PE || style="text-align:left;" | Full flowcell (~200M reads) || $4,375.00 || $4,900.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| style="text-align:left;" | 50 PE: F3 Lane (~100M reads) || $375.00 || $420.00 || per lane || rowspan="3" style="text-align:left;" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| style="text-align:left;" | Rapid 100nt: F3 flowcell (~400M reads)* || $2,250.00 || $2,520.00 || per flowcell
|-
| style="text-align:left;" | 150 PE: F3 Lane (~100M reads) || $562.50 || $630.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable" style="width:100%; text-align:center;"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
! Length !! Reads !! !! !! !!
|-
| 300nt || 5M || $893.75 || $1,001.00 || rowspan="9" | per flowcell || rowspan="9" style="text-align:left;" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,300.00 || $1,456.00
|-
| 100nt || 25M || $1,156.25 || $1,295.00
|-
| 300nt || 25M || $1,667.50 || $1,867.60
|-
| 600nt || 25M || $1,962.50 || $2,198.00
|-
| 1000nt || 25M || $3,237.50 || $3,626.00
|-
| 100nt || 100M || $1,925.00 || $2,156.00
|-
| 300nt || 100M || $2,656.00 || $2,975.00
|-
| 600nt || 50M || $2,656.00 || $2,975.00
|}

==== MiSeq i100 Walkup ====

Walkup service is not available to external users.

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable" style="width:100%; text-align:center;"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| style="text-align:left;" | 70nt V2 kit (eg. 30PE or 70SE) || $1,650.00 || $1,848.00 || rowspan="6" | per flowcell || rowspan="6" style="text-align:left;" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| style="text-align:left;" | 150nt V3 kits (eg. 75PE) || $1,787.50 || $2,002.00
|-
| style="text-align:left;" | 300nt V2 kit (eg. 150PE) || $1,993.75 || $2,233.00
|-
| style="text-align:left;" | 500nt V2 kit (eg. 250PE) || $2,200.00 || $2,464.00
|-
| style="text-align:left;" | 600nt V3 kit (eg. 300PE) || $2,750.00 || $3,080.00
|-
| style="text-align:left;" | 500nt NANO Run (eg. 250PE) || $962.50 || $1,078.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| style="text-align:left;" | Quality Control Only - only one QC is included per sequencing lane. || $43.75 || $49.00 || per sample || style="text-align:left;" | Includes RT-PCR and Frag.Analyzer
|-
| style="text-align:left;" | Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $18.75 || $21.00 || per sample || style="text-align:left;" | Includes Fluorometric quantification and Frag.Analyzer
|-
| style="text-align:left;" | qPCR only (short read) || $31.25 || $35.00 || per sample || style="text-align:left;" | Includes qPCR values from standard QC only
|-
| style="text-align:left;" | qPCR only - full plate (short read) || $500.00 || $560.00 || per plate || style="text-align:left;" | excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable" style="width:100%; text-align:center;"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| style="text-align:left;" | 72 hour Flowcell || $1,500.00 || $1,680.00 || per Flowcell || style="text-align:left;" | Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable" style="width:100%; text-align:center;"
|-

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Illumina || Singular || style="text-align:left;" | Index Overwrite (per sample) || $37.50 || $42.00 || style="text-align:left;" | Includes QC and reindexing
|-
| Illumina || Singular || style="text-align:left;" | P5/P7 conversion (per pool) || $125.00 || $140.00 || style="text-align:left;" | Includes QC. Samples not reindexed. Requires full flowcell for sequencing (Rapid)
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="3" style="text-align:left;" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. || colspan="3" style="text-align:center;" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| rowspan="3" style="vertical-align:middle; text-align:left;" | LIGATION BASED NEB UltraII || STD || per sample || $136.50 || $157.00 || style="text-align:left;" | Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| HTL || 24 || $1,125.00 || $1,260.00 || style="text-align:left;" | Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| HTL || 96 || $2,250.00 || $2,520.00
|-
| style="text-align:left;" | Tagmentation NEXTERA-XT || STD || per Sample || $136.50 || $157.00 || rowspan="2" style="text-align:left;" | Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| style="text-align:left;" | Tagmentation NEXTERA-FLEX || STD || per Sample || $204.00 || $235.00
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | NEXTERA-XT OR FLEX || HTL || 24 || $1,125.00 || $1,260.00 || style="text-align:left;" | Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| HTL || 96 || $2,250.00 || $2,520.00
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | 16S Amplicon || HTL || 48 || $2,375.00 || $2,660.00 || style="text-align:left;" | Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || add'l 48 || $812.50 || $910.00
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | Other Amplicon || HTL || 48 || $2,000.00 || $2,240.00 || style="text-align:left;" | Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || add'l 48 || N/A || $945.00
|-
| colspan="6" style="text-align:center;" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| style="text-align:left;" | High Throughput Setup || HTL || 48 samples || $75.00 || $84.00
|-
| style="text-align:left;" | Sample Cleanup || HTL || per sample || $6.25 || $7.00 || style="text-align:left;" | SPRI cleanup of samples to remove impurities.
|-
| style="text-align:left;" | Quantification || HTL || per sample || $12.50 || $14.00 || style="text-align:left;" | Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="3" style="text-align:left;" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. || colspan="3" style="text-align:center;" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| rowspan="3" style="vertical-align:middle; text-align:left;" | polyA based isolation NEB UltraII RNAseq || STD || per sample || $196.25 || $219.80 || style="text-align:left;" | Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| HTL (NEB only) || 24 || $1,875.00 || $2,100.00 || style="text-align:left;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| HTL (NEB only) || 96 || $3,750.00 || $4,200.00 ||
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | High Throughput 3' Digital Gene Expression (HT3DGE) || HTL || 24 || $1,312.50 || $1,470.00 || style="text-align:left;" | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| HTL || 96 || $2,625.00 || $2,940.00 ||
|-
| rowspan="3" style="vertical-align:middle; text-align:left;" | Total RNA sequencing NEB RNAseq +Ribodepletion || STD || per sample || $293.75 || $329.00 || style="text-align:left;" | Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| HTL (NEB only, covaris RNA fragmentation) || 24 || $3,125.00 || $3,500.00 || style="text-align:left;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || 96 || $6,250.00 || $7,000.00 ||
|-
| rowspan="3" style="vertical-align:middle; text-align:left;" | Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || STD v4 || per sample || $340.60 || $394.00 || style="text-align:left;" | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| HTL v2 || 24 || $2,500.00 || $2,800.00 ||
|-
| || 96 || $4,000.00 || $4,480.00 ||
|-
| rowspan="3" style="vertical-align:middle; text-align:left;" | Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $273.00 || $315.00 || style="text-align:left;" | Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| HTL (covaris RNA fragmentation) || 24 || $3,750.00 || $4,200.00 || style="text-align:left;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || 96 || $6,250.00 || $7,000.00 ||
|-
| style="text-align:left;" | Small RNA || STD || per sample || $375.70 || $433.00 || style="text-align:left;" | Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" style="text-align:center;" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| style="text-align:left;" | High Throughput Setup || || 48 samples || $75.00 || $84.00 ||
|-
| style="text-align:left;" | Sample Cleanup || || per sample || $6.25 || $7.00 || style="text-align:left;" | SPRI cleanup of samples to remove impurities.
|-
| style="text-align:left;" | Quantification || || per sample || $12.50 || $14.00 || style="text-align:left;" | Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| style="text-align:left;" | Standard Ligation || LSK || $200.00 || $240.00 || rowspan="6" style="text-align:left;" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| style="text-align:left;" | Native Barcoding (ligation indexing) || - || $65.00 || $70.00
|-
| style="text-align:left;" | SMARTseq -> Ligation || Cv4+LSK || $375.00 || $450.00
|-
| style="text-align:left;" | Rapid Prep (tagment - ~10-20kb) || RAD || $188.00 || $225.00
|-
| style="text-align:left;" | Long Insert Prep || RAD (modified, target >100kb) || $340.60 || $393.00
|-
| style="text-align:left;" | Direct RNA || RNA004 || $200.00 || $240.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| DNA || 96 || $875.00 || $980.00 ||
|-
| RNA || 96 || $1,000.00 || $1,120.00 ||
|-
| style="text-align:left;" | RNA/DNA Isolation - Assisted || 12 samples || $375.00 || $420.00 ||
|-
| style="text-align:left;" | RNA/DNA Isolation - Assisted || 96 samples || $1,250.00 || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="5" style="text-align:left;" | '''Assisted Service'''
|-
| colspan="5" style="text-align:left;" | '''Digital Gene Expression'''
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | 5' RNA / 3' RNA || setup || $1,268.75 || $1,421.00 || style="text-align:left;" | charge per submission
|-
| per sample || $2,188.00 || $2,450.00 || style="text-align:left;" | Includes QC of cDNA and creation of Short Read Library
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | 5' RNA / 3' RNA - on chip multiplexing || setup || $1,437.50 || $1,610.00 || style="text-align:left;" | charge per submission
|-
| per 4 samples || $2,881.00 || $3,227.00 || style="text-align:left;" | Includes QC of cDNA and creation of Short Read Library
|-
| rowspan="3" style="vertical-align:middle; text-align:left;" | Fixed RNA (Flex v2) probe based. human/mouse only || setup (up to 48 samples) || $3,375.00 || $3,780.00 || style="text-align:left;" | charge per submission
|-
| per sample (1-plex) || $337.50 || $378.00 || style="text-align:left;" | Includes QC of cDNA and creation of Short Read Library
|-
| per 4-plex || $4,875.00 || $5,460.00 ||
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) || setup || $587.50 || $658.00 || style="text-align:left;" | additional prep beyond DGE
|-
| per sample || $56.00 || $63.00 || style="text-align:left;" | Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" style="text-align:left;" | '''scATACseq'''
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | ATACseq || setup || $1,100.00 || $1,232.00 || style="text-align:left;" | charge per submission
|-
| per sample || $2,094.00 || $2,345.00 || style="text-align:left;" | Includes QC and creation of Short Read Library
|-
| colspan="5" style="text-align:left;" | '''Multiome (ATAC+3'RNA)'''
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | MULTI || setup || $2,250.00 || $2,520.00 || style="text-align:left;" | charge per submission
|-
| per sample || $3,875.00 || $4,340.00 || style="text-align:left;" | Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" style="text-align:left;" | '''Cancellation'''
|-
| style="text-align:left;" | per session || <24h notice || $250.00 || $280.00 ||
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| style="text-align:left;" | Test Array || slide || $1,217.00 || $1,363.00 || style="text-align:left;" | recommended 1x per tissue type for fresh frozen
|-
| style="text-align:left;" | Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $7,510.00 || $8,411.00 || style="text-align:left;" | Includes H&E stain images + Library Generation
|-
| style="text-align:left;" | Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $5,688.00 || $6,359.00 || style="text-align:left;" | Includes library generation only.
|-
| style="text-align:left;" | Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $9,813.00 || $10,940.00 || style="text-align:left;" | Includes library generation only.
|-
| style="text-align:left;" | QC - FFPE Scroll || per scroll || $75.00 || $84.00 || style="text-align:left;" | RNA isolation from FFPE scroll
|-
| style="text-align:left;" | QC - Fresh Frozen Scroll || per scroll || $60.00 || $66.00 || style="text-align:left;" | RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| style="text-align:left;" | Slide Only || per slide || $188.00 || $210.00 ||
|-
| style="text-align:left;" | Optimization Only || per slide || $1,000.00 || $1,120.00 ||
|-
| style="text-align:left;" | Teton Panel || per slide || $9,000.00 || $10,080.00 ||
|-
| style="text-align:left;" | Atlas Short || per slide || $6,875.00 || $7,700.00 ||
|-
| style="text-align:left;" | Antibody Add-on || per slide || $450.00 || $504.00 ||
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| style="text-align:left;" | Fragment Analyzer: RNA or DNA || per sample || $18.75 || $21.00 ||
|-
| style="text-align:left;" | Fragment Analyzer: Preloaded row || per row (12) || $100.00 || $112.00 || style="text-align:left;" | 2ul per sample in each well using plates from BMC
|-
| style="text-align:left;" | FemtoPulse: RNA or DNA || per sample || $37.50 || $42.00 ||
|-
| style="text-align:left;" | FemtoPulse: Preloaded Row || per row (12) || $200.00 || $224.00 || style="text-align:left;" | 2ul per sample in each well using plates from BMC
|-
| style="text-align:left;" | BioAnalyzer: Walkup Service || per chip || $62.50 || $70.00 ||
|-
| style="text-align:left;" | qPCR only (Short read quantification test) || per sample || $31.25 || $35.00 || style="text-align:left;" | 4 point serial dilution
|-
| style="text-align:left;" | Full plate qPCR (short read quantification test) || per plate || $500.00 || $560.00 || style="text-align:left;" | full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" style="text-align:left;" | '''Sample QC services:'''
|-
| style="text-align:left;" | Fresh Frozen Curl || per curl || $60.00 || $66.00 ||
|-
| style="text-align:left;" | FFPE Curl || per curl || $75.00 || $84.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| rowspan="3" style="vertical-align:middle;" | Dr.Oligo || style="text-align:left;" | Assisted || $2,500.00 +consumables || $2,800.00 +consumables || style="text-align:left;" | includes setup and run of the instrument only. Large reagents / N2 included.
|-
| style="text-align:left;" | Walkup || $1,250.00 +consumables || $1,400.00 +consumables || style="text-align:left;" | includes run of the instrument only. Large reagents / N2 included.
|-
| style="text-align:left;" | Training || $5,000.00 || $5,600.00 || style="text-align:left;" | includes two instrument runs.
|-
| rowspan="6" style="vertical-align:middle;" | DNAscript Syntax || style="text-align:left;" | Setup (per run) || $1,250.00 || $1,400.00 || style="text-align:left;" | Per run cost
|-
| style="text-align:left;" | 2000 nucleotide synthesis || $625.00 || $700.00 || style="text-align:left;" | Up to 96 samples
|-
| style="text-align:left;" | 8000 nucleotide synthesis || $1,875.00 || $2,100.00 || style="text-align:left;" | Up to 96 samples
|-
| style="text-align:left;" | add Biotin (max 48) || $144.00 || $161.00 || style="text-align:left;" | max 48 per run and 2 per oligo. Couple to T only.
|-
| style="text-align:left;" | add Fluor || $375.00 || $420.00 ||
|-
| style="text-align:left;" | add Quench || $125.00 || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to external users.

== OTHER LAB SERVICES ==

{| class="wikitable" style="width:100%; text-align:center;"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! SIGN UP !! Notes
|-
| colspan="6" style="text-align:left;" | '''COVARIS SONICATOR'''
|-
| style="text-align:left;" | COVARIS E220 || $25.00 || $28.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || style="text-align:left;" | tubes available for purchase.
|-
| colspan="6" style="text-align:left;" | '''PIPPIN PREP'''
|-
| style="text-align:left;" | Gel Isolation || $93.75 || $105.00 || per gel (5 lanes) || TBA ||
|-
| colspan="6" style="text-align:left;" | '''PLATE READER'''
|-
| style="text-align:left;" | Varioskan || $23.00 || $27.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || style="text-align:left;" | Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="6" style="text-align:left;" | '''ROBOTICS'''
|-
| style="text-align:left;" | Tecan EVO 150 || $93.75 || $105.00 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| style="text-align:left;" | non-filter tips || $10.00 || $11.20 || per box || || style="text-align:left;" | ** Please let us know if there is additional plasticware you would like us to stock.
|-
| style="text-align:left;" | Filter tips || $15.00 || $16.80 || per box || ||
|-
| style="text-align:left;" | Training || $81.25 || $91.00 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]] ||
|-
| colspan="6" style="text-align:left;" | '''NANODROP'''
|-
| style="text-align:left;" | Nanodrop || -- || -- || || Walk up service || style="text-align:left;" | * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable" style="width:100%; text-align:center;"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| style="text-align:left;" | Informatics Support || per hour || $180.00 || $205.00 || style="text-align:left;" | Very limited support for external users
|-
| style="text-align:left;" | Ingenuity Pathway Analysis || per dataset || N/A || $150.00 || style="text-align:left;" | Recovery of licence cost.
|}

BioMicroCenter:Pricing

2026-04-17T15:27:06Z

Cstjohn: /* NOVASEQX / NOVASEQ6000 SEQUENCING */

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| style="text-align:left;" | NovaSeq SP 100nt || $4,623.75 || $5,178.60 || Per flowcell || rowspan="9" style="text-align:left;" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| style="text-align:left;" | NovaSeq SP 300nt** || $6,547.50 || $7,333.20
|-
| style="text-align:left;" | NovaSeq SP 500nt || $8,775.00 || $9,828.00
|-
| style="text-align:left;" | NovaSeq S1 100nt** || $7,290.00 || $8,164.80
|-
| style="text-align:left;" | NovaSeq S1 200nt** || $9,315.00 || $10,432.80
|-
| style="text-align:left;" | NovaSeq S1 300nt** || $10,158.75 || $11,377.80
|-
| style="text-align:left;" | NovaSeq S2 100nt** || $12,993.75 || $14,553.00
|-
| style="text-align:left;" | NovaSeq S2 200nt** || $16,200.00 || $18,144.00
|-
| style="text-align:left;" | NovaSeq S2 300nt** || $16,875.00 || $18,900.00
|-
| style="text-align:left;" | NovaSeq S4 50nt || $4,725.00 || $5,292.00 || Per Lane || rowspan="2" style="text-align:left;" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| style="text-align:left;" | NovaSeq S4 300nt || $6,750.00 || $7,560.00
|-
| style="text-align:left;" | NovaSeqX 10B 150PE Lane^^ || $2,312.50 || $2,590.00 || Per Lane || rowspan="5" style="text-align:left;" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| style="text-align:left;" | NovaSeqX 25B 150PE Lane^^ || $3,250.00 || $3,640.00
|-
| style="text-align:left;" | NovaSeqX 10B 300nt Flowcell^^ || $15,200.00 || $17,024.00 || Per Flowcell
|-
| style="text-align:left;" | NovaSeqX 25B 300nt Flowcell^^ || $20,250.00 || $22,680.00
|-
| style="text-align:left;" | NovaSeqX 25B 100nt Flowcell^^ || $7,187.50 || $8,050.00
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| rowspan="2" style="vertical-align:middle;" | 75 PE || style="text-align:left;" | Full flowcell (2 lane, ~800M reads) || $2,375.00 || $2,660.00 || per flowcell || rowspan="5" style="text-align:left;" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| style="text-align:left;" | One lane (~400M reads) || $1,250.00 || $1,400.00 || per lane
|-
| rowspan="2" style="vertical-align:middle;" | 150 PE || style="text-align:left;" | Full flowcell (2 lane, ~800M reads) || $3,312.50 || $3,710.00 || per flowcell
|-
| style="text-align:left;" | One lane (~400M reads) || $1,719.00 || $1,925.00 || per lane
|-
| 300 PE || style="text-align:left;" | Full flowcell (~200M reads) || $4,375.00 || $4,900.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| style="text-align:left;" | 50 PE: F3 Lane (~100M reads) || $375.00 || $420.00 || per lane || rowspan="3" style="text-align:left;" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| style="text-align:left;" | Rapid 100nt: F3 flowcell (~400M reads)* || $2,250.00 || $2,520.00 || per flowcell
|-
| style="text-align:left;" | 150 PE: F3 Lane (~100M reads) || $562.50 || $630.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable" style="width:100%; text-align:center;"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
! Length !! Reads !! !! !! !!
|-
| 300nt || 5M || $893.75 || $1,001.00 || rowspan="9" | per flowcell || rowspan="9" style="text-align:left;" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,300.00 || $1,456.00
|-
| 100nt || 25M || $1,156.25 || $1,295.00
|-
| 300nt || 25M || $1,667.50 || $1,867.60
|-
| 600nt || 25M || $1,962.50 || $2,198.00
|-
| 1000nt || 25M || $3,237.50 || $3,626.00
|-
| 100nt || 100M || $1,925.00 || $2,156.00
|-
| 300nt || 100M || $2,656.00 || $2,975.00
|-
| 600nt || 50M || $2,656.00 || $2,975.00
|}

==== MiSeq i100 Walkup ====

Walkup service is not available to external users.

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable" style="width:100%; text-align:center;"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| style="text-align:left;" | 70nt V2 kit (eg. 30PE or 70SE) || $1,650.00 || $1,848.00 || rowspan="6" | per flowcell || rowspan="6" style="text-align:left;" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| style="text-align:left;" | 150nt V3 kits (eg. 75PE) || $1,787.50 || $2,002.00
|-
| style="text-align:left;" | 300nt V2 kit (eg. 150PE) || $1,993.75 || $2,233.00
|-
| style="text-align:left;" | 500nt V2 kit (eg. 250PE) || $2,200.00 || $2,464.00
|-
| style="text-align:left;" | 600nt V3 kit (eg. 300PE) || $2,750.00 || $3,080.00
|-
| style="text-align:left;" | 500nt NANO Run (eg. 250PE) || $962.50 || $1,078.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| style="text-align:left;" | Quality Control Only - only one QC is included per sequencing lane. || $43.75 || $49.00 || per sample || style="text-align:left;" | Includes RT-PCR and Frag.Analyzer
|-
| style="text-align:left;" | Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $18.75 || $21.00 || per sample || style="text-align:left;" | Includes Fluorometric quantification and Frag.Analyzer
|-
| style="text-align:left;" | qPCR only (short read) || $31.25 || $35.00 || per sample || style="text-align:left;" | Includes qPCR values from standard QC only
|-
| style="text-align:left;" | qPCR only - full plate (short read) || $500.00 || $560.00 || per plate || style="text-align:left;" | excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable" style="width:100%; text-align:center;"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| style="text-align:left;" | 72 hour Flowcell || $1,500.00 || $1,680.00 || per Flowcell || style="text-align:left;" | Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable" style="width:100%; text-align:center;"
|-

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Illumina || Singular || style="text-align:left;" | Index Overwrite (per sample) || $37.50 || $42.00 || style="text-align:left;" | Includes QC and reindexing
|-
| Illumina || Singular || style="text-align:left;" | P5/P7 conversion (per pool) || $125.00 || $140.00 || style="text-align:left;" | Includes QC. Samples not reindexed. Requires full flowcell for sequencing (Rapid)
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="3" style="text-align:left;" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. || colspan="3" style="text-align:center;" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| rowspan="3" style="vertical-align:middle; text-align:left;" | LIGATION BASED NEB UltraII || STD || per sample || $136.50 || $157.00 || style="text-align:left;" | Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| HTL || 24 || $1,125.00 || $1,260.00 || style="text-align:left;" | Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| HTL || 96 || $2,250.00 || $2,520.00 ||
|-
| style="text-align:left;" | Tagmentation NEXTERA-XT || STD || per Sample || $136.50 || $157.00 || rowspan="2" style="text-align:left;" | Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| style="text-align:left;" | Tagmentation NEXTERA-FLEX || STD || per Sample || $204.00 || $235.00
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | NEXTERA-XT OR FLEX || HTL || 24 || $1,125.00 || $1,260.00 || style="text-align:left;" | Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| HTL || 96 || $2,250.00 || $2,520.00 ||
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | 16S Amplicon || HTL || 48 || $2,375.00 || $2,660.00 || style="text-align:left;" | Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || add'l 48 || $812.50 || $910.00 ||
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | Other Amplicon || HTL || 48 || $2,000.00 || $2,240.00 || style="text-align:left;" | Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || add'l 48 || N/A || $945.00 ||
|-
| colspan="6" style="text-align:center;" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| style="text-align:left;" | High Throughput Setup || || 48 samples || $75.00 || $84.00 ||
|-
| style="text-align:left;" | Sample Cleanup || || per sample || $6.25 || $7.00 || style="text-align:left;" | SPRI cleanup of samples to remove impurities.
|-
| style="text-align:left;" | Quantification || || per sample || $12.50 || $14.00 || style="text-align:left;" | Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="3" style="text-align:left;" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. || colspan="3" style="text-align:center;" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| rowspan="3" style="vertical-align:middle; text-align:left;" | polyA based isolation NEB UltraII RNAseq || STD || per sample || $196.25 || $219.80 || style="text-align:left;" | Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| HTL (NEB only) || 24 || $1,875.00 || $2,100.00 || style="text-align:left;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| HTL (NEB only) || 96 || $3,750.00 || $4,200.00 ||
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | High Throughput 3' Digital Gene Expression (HT3DGE) || HTL || 24 || $1,312.50 || $1,470.00 || style="text-align:left;" | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| HTL || 96 || $2,625.00 || $2,940.00 ||
|-
| rowspan="3" style="vertical-align:middle; text-align:left;" | Total RNA sequencing NEB RNAseq +Ribodepletion || STD || per sample || $293.75 || $329.00 || style="text-align:left;" | Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| HTL (NEB only, covaris RNA fragmentation) || 24 || $3,125.00 || $3,500.00 || style="text-align:left;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || 96 || $6,250.00 || $7,000.00 ||
|-
| rowspan="3" style="vertical-align:middle; text-align:left;" | Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || STD v4 || per sample || $340.60 || $394.00 || style="text-align:left;" | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| HTL v2 || 24 || $2,500.00 || $2,800.00 ||
|-
| || 96 || $4,000.00 || $4,480.00 ||
|-
| rowspan="3" style="vertical-align:middle; text-align:left;" | Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $273.00 || $315.00 || style="text-align:left;" | Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| HTL (covaris RNA fragmentation) || 24 || $3,750.00 || $4,200.00 || style="text-align:left;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || 96 || $6,250.00 || $7,000.00 ||
|-
| style="text-align:left;" | Small RNA || STD || per sample || $375.70 || $433.00 || style="text-align:left;" | Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" style="text-align:center;" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| style="text-align:left;" | High Throughput Setup || || 48 samples || $75.00 || $84.00 ||
|-
| style="text-align:left;" | Sample Cleanup || || per sample || $6.25 || $7.00 || style="text-align:left;" | SPRI cleanup of samples to remove impurities.
|-
| style="text-align:left;" | Quantification || || per sample || $12.50 || $14.00 || style="text-align:left;" | Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| style="text-align:left;" | Standard Ligation || LSK || $200.00 || $240.00 || rowspan="6" style="text-align:left;" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| style="text-align:left;" | Native Barcoding (ligation indexing) || - || $65.00 || $70.00
|-
| style="text-align:left;" | SMARTseq -> Ligation || Cv4+LSK || $375.00 || $450.00
|-
| style="text-align:left;" | Rapid Prep (tagment - ~10-20kb) || RAD || $188.00 || $225.00
|-
| style="text-align:left;" | Long Insert Prep || RAD (modified, target >100kb) || $340.60 || $393.00
|-
| style="text-align:left;" | Direct RNA || RNA004 || $200.00 || $240.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| DNA || 96 || $875.00 || $980.00 ||
|-
| RNA || 96 || $1,000.00 || $1,120.00 ||
|-
| style="text-align:left;" | RNA/DNA Isolation - Assisted || 12 samples || $375.00 || $420.00 ||
|-
| style="text-align:left;" | RNA/DNA Isolation - Assisted || 96 samples || $1,250.00 || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="5" style="text-align:left;" | '''Assisted Service'''
|-
| colspan="5" style="text-align:left;" | '''Digital Gene Expression'''
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | 5' RNA / 3' RNA || setup || $1,268.75 || $1,421.00 || style="text-align:left;" | charge per submission
|-
| per sample || $2,188.00 || $2,450.00 || style="text-align:left;" | Includes QC of cDNA and creation of Short Read Library
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | 5' RNA / 3' RNA - on chip multiplexing || setup || $1,437.50 || $1,610.00 || style="text-align:left;" | charge per submission
|-
| per 4 samples || $2,881.00 || $3,227.00 || style="text-align:left;" | Includes QC of cDNA and creation of Short Read Library
|-
| rowspan="3" style="vertical-align:middle; text-align:left;" | Fixed RNA (Flex v2) probe based. human/mouse only || setup (up to 48 samples) || $3,375.00 || $3,780.00 || style="text-align:left;" | charge per submission
|-
| per sample (1-plex) || $337.50 || $378.00 || style="text-align:left;" | Includes QC of cDNA and creation of Short Read Library
|-
| per 4-plex || $4,875.00 || $5,460.00 ||
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) || setup || $587.50 || $658.00 || style="text-align:left;" | additional prep beyond DGE
|-
| per sample || $56.00 || $63.00 || style="text-align:left;" | Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" style="text-align:left;" | '''scATACseq'''
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | ATACseq || setup || $1,100.00 || $1,232.00 || style="text-align:left;" | charge per submission
|-
| per sample || $2,094.00 || $2,345.00 || style="text-align:left;" | Includes QC and creation of Short Read Library
|-
| colspan="5" style="text-align:left;" | '''Multiome (ATAC+3'RNA)'''
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | MULTI || setup || $2,250.00 || $2,520.00 || style="text-align:left;" | charge per submission
|-
| per sample || $3,875.00 || $4,340.00 || style="text-align:left;" | Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" style="text-align:left;" | '''Cancellation'''
|-
| style="text-align:left;" | per session || <24h notice || $250.00 || $280.00 ||
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| style="text-align:left;" | Test Array || slide || $1,217.00 || $1,363.00 || style="text-align:left;" | recommended 1x per tissue type for fresh frozen
|-
| style="text-align:left;" | Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $7,510.00 || $8,411.00 || style="text-align:left;" | Includes H&E stain images + Library Generation
|-
| style="text-align:left;" | Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $5,688.00 || $6,359.00 || style="text-align:left;" | Includes library generation only.
|-
| style="text-align:left;" | Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $9,813.00 || $10,940.00 || style="text-align:left;" | Includes library generation only.
|-
| style="text-align:left;" | QC - FFPE Scroll || per scroll || $75.00 || $84.00 || style="text-align:left;" | RNA isolation from FFPE scroll
|-
| style="text-align:left;" | QC - Fresh Frozen Scroll || per scroll || $60.00 || $66.00 || style="text-align:left;" | RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| style="text-align:left;" | Slide Only || per slide || $188.00 || $210.00 ||
|-
| style="text-align:left;" | Optimization Only || per slide || $1,000.00 || $1,120.00 ||
|-
| style="text-align:left;" | Teton Panel || per slide || $9,000.00 || $10,080.00 ||
|-
| style="text-align:left;" | Atlas Short || per slide || $6,875.00 || $7,700.00 ||
|-
| style="text-align:left;" | Antibody Add-on || per slide || $450.00 || $504.00 ||
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| style="text-align:left;" | Fragment Analyzer: RNA or DNA || per sample || $18.75 || $21.00 ||
|-
| style="text-align:left;" | Fragment Analyzer: Preloaded row || per row (12) || $100.00 || $112.00 || style="text-align:left;" | 2ul per sample in each well using plates from BMC
|-
| style="text-align:left;" | FemtoPulse: RNA or DNA || per sample || $37.50 || $42.00 ||
|-
| style="text-align:left;" | FemtoPulse: Preloaded Row || per row (12) || $200.00 || $224.00 || style="text-align:left;" | 2ul per sample in each well using plates from BMC
|-
| style="text-align:left;" | BioAnalyzer: Walkup Service || per chip || $62.50 || $70.00 ||
|-
| style="text-align:left;" | qPCR only (Short read quantification test) || per sample || $31.25 || $35.00 || style="text-align:left;" | 4 point serial dilution
|-
| style="text-align:left;" | Full plate qPCR (short read quantification test) || per plate || $500.00 || $560.00 || style="text-align:left;" | full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" style="text-align:left;" | '''Sample QC services:'''
|-
| style="text-align:left;" | Fresh Frozen Curl || per curl || $60.00 || $66.00 ||
|-
| style="text-align:left;" | FFPE Curl || per curl || $75.00 || $84.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| rowspan="3" style="vertical-align:middle;" | Dr.Oligo || style="text-align:left;" | Assisted || $2,500.00 +consumables || $2,800.00 +consumables || style="text-align:left;" | includes setup and run of the instrument only. Large reagents / N2 included.
|-
| style="text-align:left;" | Walkup || $1,250.00 +consumables || $1,400.00 +consumables || style="text-align:left;" | includes run of the instrument only. Large reagents / N2 included.
|-
| style="text-align:left;" | Training || $5,000.00 || $5,600.00 || style="text-align:left;" | includes two instrument runs.
|-
| rowspan="6" style="vertical-align:middle;" | DNAscript Syntax || style="text-align:left;" | Setup (per run) || $1,250.00 || $1,400.00 || style="text-align:left;" | Per run cost
|-
| style="text-align:left;" | 2000 nucleotide synthesis || $625.00 || $700.00 || style="text-align:left;" | Up to 96 samples
|-
| style="text-align:left;" | 8000 nucleotide synthesis || $1,875.00 || $2,100.00 || style="text-align:left;" | Up to 96 samples
|-
| style="text-align:left;" | add Biotin (max 48) || $144.00 || $161.00 || style="text-align:left;" | max 48 per run and 2 per oligo. Couple to T only.
|-
| style="text-align:left;" | add Fluor || $375.00 || $420.00 ||
|-
| style="text-align:left;" | add Quench || $125.00 || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to external users.

== OTHER LAB SERVICES ==

{| class="wikitable" style="width:100%; text-align:center;"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! SIGN UP !! Notes
|-
| colspan="6" style="text-align:left;" | '''COVARIS SONICATOR'''
|-
| style="text-align:left;" | COVARIS E220 || $25.00 || $28.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || style="text-align:left;" | tubes available for purchase.
|-
| colspan="6" style="text-align:left;" | '''PIPPIN PREP'''
|-
| style="text-align:left;" | Gel Isolation || $93.75 || $105.00 || per gel (5 lanes) || TBA ||
|-
| colspan="6" style="text-align:left;" | '''PLATE READER'''
|-
| style="text-align:left;" | Varioskan || $23.00 || $27.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || style="text-align:left;" | Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="6" style="text-align:left;" | '''ROBOTICS'''
|-
| style="text-align:left;" | Tecan EVO 150 || $93.75 || $105.00 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| style="text-align:left;" | non-filter tips || $10.00 || $11.20 || per box || || style="text-align:left;" | ** Please let us know if there is additional plasticware you would like us to stock.
|-
| style="text-align:left;" | Filter tips || $15.00 || $16.80 || per box || ||
|-
| style="text-align:left;" | Training || $81.25 || $91.00 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]] ||
|-
| colspan="6" style="text-align:left;" | '''NANODROP'''
|-
| style="text-align:left;" | Nanodrop || -- || -- || || Walk up service || style="text-align:left;" | * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable" style="width:100%; text-align:center;"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| style="text-align:left;" | Informatics Support || per hour || $180.00 || $205.00 || style="text-align:left;" | Very limited support for external users
|-
| style="text-align:left;" | Ingenuity Pathway Analysis || per dataset || N/A || $150.00 || style="text-align:left;" | Recovery of licence cost.
|}

BioMicroCenter:Pricing

2026-04-17T15:26:22Z

Cstjohn:

== SEQUENCING - HIGH OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== NOVASEQX / NOVASEQ6000 SEQUENCING ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| style="text-align:left;" | NovaSeq SP 100nt || $4,623.75 || $5,178.60 || Per flowcell || rowspan="9" style="text-align:left;" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| style="text-align:left;" | NovaSeq SP 300nt** || $6,547.50 || $7,333.20 ||
|-
| style="text-align:left;" | NovaSeq SP 500nt || $8,775.00 || $9,828.00 ||
|-
| style="text-align:left;" | NovaSeq S1 100nt** || $7,290.00 || $8,164.80 ||
|-
| style="text-align:left;" | NovaSeq S1 200nt** || $9,315.00 || $10,432.80 ||
|-
| style="text-align:left;" | NovaSeq S1 300nt** || $10,158.75 || $11,377.80 ||
|-
| style="text-align:left;" | NovaSeq S2 100nt** || $12,993.75 || $14,553.00 ||
|-
| style="text-align:left;" | NovaSeq S2 200nt** || $16,200.00 || $18,144.00 ||
|-
| style="text-align:left;" | NovaSeq S2 300nt** || $16,875.00 || $18,900.00 ||
|-
| style="text-align:left;" | NovaSeq S4 50nt || $4,725.00 || $5,292.00 || Per Lane || rowspan="2" style="text-align:left;" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| style="text-align:left;" | NovaSeq S4 300nt || $6,750.00 || $7,560.00 ||
|-
| style="text-align:left;" | NovaSeqX 10B 150PE Lane^^ || $2,312.50 || $2,590.00 || Per Lane || rowspan="5" style="text-align:left;" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| style="text-align:left;" | NovaSeqX 25B 150PE Lane^^ || $3,250.00 || $3,640.00 ||
|-
| style="text-align:left;" | NovaSeqX 10B 300nt Flowcell^^ || $15,200.00 || $17,024.00 || Per Flowcell ||
|-
| style="text-align:left;" | NovaSeqX 25B 300nt Flowcell^^ || $20,250.00 || $22,680.00 ||
|-
| style="text-align:left;" | NovaSeqX 25B 100nt Flowcell^^ || $7,187.50 || $8,050.00 ||
|}

<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| rowspan="2" style="vertical-align:middle;" | 75 PE || style="text-align:left;" | Full flowcell (2 lane, ~800M reads) || $2,375.00 || $2,660.00 || per flowcell || rowspan="5" style="text-align:left;" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| style="text-align:left;" | One lane (~400M reads) || $1,250.00 || $1,400.00 || per lane
|-
| rowspan="2" style="vertical-align:middle;" | 150 PE || style="text-align:left;" | Full flowcell (2 lane, ~800M reads) || $3,312.50 || $3,710.00 || per flowcell
|-
| style="text-align:left;" | One lane (~400M reads) || $1,719.00 || $1,925.00 || per lane
|-
| 300 PE || style="text-align:left;" | Full flowcell (~200M reads) || $4,375.00 || $4,900.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| style="text-align:left;" | 50 PE: F3 Lane (~100M reads) || $375.00 || $420.00 || per lane || rowspan="3" style="text-align:left;" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| style="text-align:left;" | Rapid 100nt: F3 flowcell (~400M reads)* || $2,250.00 || $2,520.00 || per flowcell
|-
| style="text-align:left;" | 150 PE: F3 Lane (~100M reads) || $562.50 || $630.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable" style="width:100%; text-align:center;"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
! Length !! Reads !! !! !! !!
|-
| 300nt || 5M || $893.75 || $1,001.00 || rowspan="9" | per flowcell || rowspan="9" style="text-align:left;" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,300.00 || $1,456.00
|-
| 100nt || 25M || $1,156.25 || $1,295.00
|-
| 300nt || 25M || $1,667.50 || $1,867.60
|-
| 600nt || 25M || $1,962.50 || $2,198.00
|-
| 1000nt || 25M || $3,237.50 || $3,626.00
|-
| 100nt || 100M || $1,925.00 || $2,156.00
|-
| 300nt || 100M || $2,656.00 || $2,975.00
|-
| 600nt || 50M || $2,656.00 || $2,975.00
|}

==== MiSeq i100 Walkup ====

Walkup service is not available to external users.

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable" style="width:100%; text-align:center;"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| style="text-align:left;" | 70nt V2 kit (eg. 30PE or 70SE) || $1,650.00 || $1,848.00 || rowspan="6" | per flowcell || rowspan="6" style="text-align:left;" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| style="text-align:left;" | 150nt V3 kits (eg. 75PE) || $1,787.50 || $2,002.00
|-
| style="text-align:left;" | 300nt V2 kit (eg. 150PE) || $1,993.75 || $2,233.00
|-
| style="text-align:left;" | 500nt V2 kit (eg. 250PE) || $2,200.00 || $2,464.00
|-
| style="text-align:left;" | 600nt V3 kit (eg. 300PE) || $2,750.00 || $3,080.00
|-
| style="text-align:left;" | 500nt NANO Run (eg. 250PE) || $962.50 || $1,078.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| style="text-align:left;" | Quality Control Only - only one QC is included per sequencing lane. || $43.75 || $49.00 || per sample || style="text-align:left;" | Includes RT-PCR and Frag.Analyzer
|-
| style="text-align:left;" | Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $18.75 || $21.00 || per sample || style="text-align:left;" | Includes Fluorometric quantification and Frag.Analyzer
|-
| style="text-align:left;" | qPCR only (short read) || $31.25 || $35.00 || per sample || style="text-align:left;" | Includes qPCR values from standard QC only
|-
| style="text-align:left;" | qPCR only - full plate (short read) || $500.00 || $560.00 || per plate || style="text-align:left;" | excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable" style="width:100%; text-align:center;"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| style="text-align:left;" | 72 hour Flowcell || $1,500.00 || $1,680.00 || per Flowcell || style="text-align:left;" | Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable" style="width:100%; text-align:center;"
|-

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Illumina || Singular || style="text-align:left;" | Index Overwrite (per sample) || $37.50 || $42.00 || style="text-align:left;" | Includes QC and reindexing
|-
| Illumina || Singular || style="text-align:left;" | P5/P7 conversion (per pool) || $125.00 || $140.00 || style="text-align:left;" | Includes QC. Samples not reindexed. Requires full flowcell for sequencing (Rapid)
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="3" style="text-align:left;" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. || colspan="3" style="text-align:center;" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| rowspan="3" style="vertical-align:middle; text-align:left;" | LIGATION BASED NEB UltraII || STD || per sample || $136.50 || $157.00 || style="text-align:left;" | Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| HTL || 24 || $1,125.00 || $1,260.00 || style="text-align:left;" | Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| HTL || 96 || $2,250.00 || $2,520.00 ||
|-
| style="text-align:left;" | Tagmentation NEXTERA-XT || STD || per Sample || $136.50 || $157.00 || rowspan="2" style="text-align:left;" | Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| style="text-align:left;" | Tagmentation NEXTERA-FLEX || STD || per Sample || $204.00 || $235.00
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | NEXTERA-XT OR FLEX || HTL || 24 || $1,125.00 || $1,260.00 || style="text-align:left;" | Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| HTL || 96 || $2,250.00 || $2,520.00 ||
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | 16S Amplicon || HTL || 48 || $2,375.00 || $2,660.00 || style="text-align:left;" | Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || add'l 48 || $812.50 || $910.00 ||
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | Other Amplicon || HTL || 48 || $2,000.00 || $2,240.00 || style="text-align:left;" | Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || add'l 48 || N/A || $945.00 ||
|-
| colspan="6" style="text-align:center;" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| style="text-align:left;" | High Throughput Setup || || 48 samples || $75.00 || $84.00 ||
|-
| style="text-align:left;" | Sample Cleanup || || per sample || $6.25 || $7.00 || style="text-align:left;" | SPRI cleanup of samples to remove impurities.
|-
| style="text-align:left;" | Quantification || || per sample || $12.50 || $14.00 || style="text-align:left;" | Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="3" style="text-align:left;" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. || colspan="3" style="text-align:center;" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| rowspan="3" style="vertical-align:middle; text-align:left;" | polyA based isolation NEB UltraII RNAseq || STD || per sample || $196.25 || $219.80 || style="text-align:left;" | Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| HTL (NEB only) || 24 || $1,875.00 || $2,100.00 || style="text-align:left;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| HTL (NEB only) || 96 || $3,750.00 || $4,200.00 ||
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | High Throughput 3' Digital Gene Expression (HT3DGE) || HTL || 24 || $1,312.50 || $1,470.00 || style="text-align:left;" | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| HTL || 96 || $2,625.00 || $2,940.00 ||
|-
| rowspan="3" style="vertical-align:middle; text-align:left;" | Total RNA sequencing NEB RNAseq +Ribodepletion || STD || per sample || $293.75 || $329.00 || style="text-align:left;" | Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| HTL (NEB only, covaris RNA fragmentation) || 24 || $3,125.00 || $3,500.00 || style="text-align:left;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || 96 || $6,250.00 || $7,000.00 ||
|-
| rowspan="3" style="vertical-align:middle; text-align:left;" | Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || STD v4 || per sample || $340.60 || $394.00 || style="text-align:left;" | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| HTL v2 || 24 || $2,500.00 || $2,800.00 ||
|-
| || 96 || $4,000.00 || $4,480.00 ||
|-
| rowspan="3" style="vertical-align:middle; text-align:left;" | Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $273.00 || $315.00 || style="text-align:left;" | Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| HTL (covaris RNA fragmentation) || 24 || $3,750.00 || $4,200.00 || style="text-align:left;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || 96 || $6,250.00 || $7,000.00 ||
|-
| style="text-align:left;" | Small RNA || STD || per sample || $375.70 || $433.00 || style="text-align:left;" | Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" style="text-align:center;" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| style="text-align:left;" | High Throughput Setup || || 48 samples || $75.00 || $84.00 ||
|-
| style="text-align:left;" | Sample Cleanup || || per sample || $6.25 || $7.00 || style="text-align:left;" | SPRI cleanup of samples to remove impurities.
|-
| style="text-align:left;" | Quantification || || per sample || $12.50 || $14.00 || style="text-align:left;" | Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| style="text-align:left;" | Standard Ligation || LSK || $200.00 || $240.00 || rowspan="6" style="text-align:left;" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| style="text-align:left;" | Native Barcoding (ligation indexing) || - || $65.00 || $70.00
|-
| style="text-align:left;" | SMARTseq -> Ligation || Cv4+LSK || $375.00 || $450.00
|-
| style="text-align:left;" | Rapid Prep (tagment - ~10-20kb) || RAD || $188.00 || $225.00
|-
| style="text-align:left;" | Long Insert Prep || RAD (modified, target >100kb) || $340.60 || $393.00
|-
| style="text-align:left;" | Direct RNA || RNA004 || $200.00 || $240.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| DNA || 96 || $875.00 || $980.00 ||
|-
| RNA || 96 || $1,000.00 || $1,120.00 ||
|-
| style="text-align:left;" | RNA/DNA Isolation - Assisted || 12 samples || $375.00 || $420.00 ||
|-
| style="text-align:left;" | RNA/DNA Isolation - Assisted || 96 samples || $1,250.00 || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="5" style="text-align:left;" | '''Assisted Service'''
|-
| colspan="5" style="text-align:left;" | '''Digital Gene Expression'''
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | 5' RNA / 3' RNA || setup || $1,268.75 || $1,421.00 || style="text-align:left;" | charge per submission
|-
| per sample || $2,188.00 || $2,450.00 || style="text-align:left;" | Includes QC of cDNA and creation of Short Read Library
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | 5' RNA / 3' RNA - on chip multiplexing || setup || $1,437.50 || $1,610.00 || style="text-align:left;" | charge per submission
|-
| per 4 samples || $2,881.00 || $3,227.00 || style="text-align:left;" | Includes QC of cDNA and creation of Short Read Library
|-
| rowspan="3" style="vertical-align:middle; text-align:left;" | Fixed RNA (Flex v2) probe based. human/mouse only || setup (up to 48 samples) || $3,375.00 || $3,780.00 || style="text-align:left;" | charge per submission
|-
| per sample (1-plex) || $337.50 || $378.00 || style="text-align:left;" | Includes QC of cDNA and creation of Short Read Library
|-
| per 4-plex || $4,875.00 || $5,460.00 ||
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) || setup || $587.50 || $658.00 || style="text-align:left;" | additional prep beyond DGE
|-
| per sample || $56.00 || $63.00 || style="text-align:left;" | Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" style="text-align:left;" | '''scATACseq'''
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | ATACseq || setup || $1,100.00 || $1,232.00 || style="text-align:left;" | charge per submission
|-
| per sample || $2,094.00 || $2,345.00 || style="text-align:left;" | Includes QC and creation of Short Read Library
|-
| colspan="5" style="text-align:left;" | '''Multiome (ATAC+3'RNA)'''
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | MULTI || setup || $2,250.00 || $2,520.00 || style="text-align:left;" | charge per submission
|-
| per sample || $3,875.00 || $4,340.00 || style="text-align:left;" | Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" style="text-align:left;" | '''Cancellation'''
|-
| style="text-align:left;" | per session || <24h notice || $250.00 || $280.00 ||
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| style="text-align:left;" | Test Array || slide || $1,217.00 || $1,363.00 || style="text-align:left;" | recommended 1x per tissue type for fresh frozen
|-
| style="text-align:left;" | Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $7,510.00 || $8,411.00 || style="text-align:left;" | Includes H&E stain images + Library Generation
|-
| style="text-align:left;" | Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $5,688.00 || $6,359.00 || style="text-align:left;" | Includes library generation only.
|-
| style="text-align:left;" | Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $9,813.00 || $10,940.00 || style="text-align:left;" | Includes library generation only.
|-
| style="text-align:left;" | QC - FFPE Scroll || per scroll || $75.00 || $84.00 || style="text-align:left;" | RNA isolation from FFPE scroll
|-
| style="text-align:left;" | QC - Fresh Frozen Scroll || per scroll || $60.00 || $66.00 || style="text-align:left;" | RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| style="text-align:left;" | Slide Only || per slide || $188.00 || $210.00 ||
|-
| style="text-align:left;" | Optimization Only || per slide || $1,000.00 || $1,120.00 ||
|-
| style="text-align:left;" | Teton Panel || per slide || $9,000.00 || $10,080.00 ||
|-
| style="text-align:left;" | Atlas Short || per slide || $6,875.00 || $7,700.00 ||
|-
| style="text-align:left;" | Antibody Add-on || per slide || $450.00 || $504.00 ||
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| style="text-align:left;" | Fragment Analyzer: RNA or DNA || per sample || $18.75 || $21.00 ||
|-
| style="text-align:left;" | Fragment Analyzer: Preloaded row || per row (12) || $100.00 || $112.00 || style="text-align:left;" | 2ul per sample in each well using plates from BMC
|-
| style="text-align:left;" | FemtoPulse: RNA or DNA || per sample || $37.50 || $42.00 ||
|-
| style="text-align:left;" | FemtoPulse: Preloaded Row || per row (12) || $200.00 || $224.00 || style="text-align:left;" | 2ul per sample in each well using plates from BMC
|-
| style="text-align:left;" | BioAnalyzer: Walkup Service || per chip || $62.50 || $70.00 ||
|-
| style="text-align:left;" | qPCR only (Short read quantification test) || per sample || $31.25 || $35.00 || style="text-align:left;" | 4 point serial dilution
|-
| style="text-align:left;" | Full plate qPCR (short read quantification test) || per plate || $500.00 || $560.00 || style="text-align:left;" | full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" style="text-align:left;" | '''Sample QC services:'''
|-
| style="text-align:left;" | Fresh Frozen Curl || per curl || $60.00 || $66.00 ||
|-
| style="text-align:left;" | FFPE Curl || per curl || $75.00 || $84.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable" style="width:100%; text-align:center;"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| rowspan="3" style="vertical-align:middle;" | Dr.Oligo || style="text-align:left;" | Assisted || $2,500.00 +consumables || $2,800.00 +consumables || style="text-align:left;" | includes setup and run of the instrument only. Large reagents / N2 included.
|-
| style="text-align:left;" | Walkup || $1,250.00 +consumables || $1,400.00 +consumables || style="text-align:left;" | includes run of the instrument only. Large reagents / N2 included.
|-
| style="text-align:left;" | Training || $5,000.00 || $5,600.00 || style="text-align:left;" | includes two instrument runs.
|-
| rowspan="6" style="vertical-align:middle;" | DNAscript Syntax || style="text-align:left;" | Setup (per run) || $1,250.00 || $1,400.00 || style="text-align:left;" | Per run cost
|-
| style="text-align:left;" | 2000 nucleotide synthesis || $625.00 || $700.00 || style="text-align:left;" | Up to 96 samples
|-
| style="text-align:left;" | 8000 nucleotide synthesis || $1,875.00 || $2,100.00 || style="text-align:left;" | Up to 96 samples
|-
| style="text-align:left;" | add Biotin (max 48) || $144.00 || $161.00 || style="text-align:left;" | max 48 per run and 2 per oligo. Couple to T only.
|-
| style="text-align:left;" | add Fluor || $375.00 || $420.00 ||
|-
| style="text-align:left;" | add Quench || $125.00 || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to external users.

== OTHER LAB SERVICES ==

{| class="wikitable" style="width:100%; text-align:center;"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! SIGN UP !! Notes
|-
| colspan="6" style="text-align:left;" | '''COVARIS SONICATOR'''
|-
| style="text-align:left;" | COVARIS E220 || $25.00 || $28.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || style="text-align:left;" | tubes available for purchase.
|-
| colspan="6" style="text-align:left;" | '''PIPPIN PREP'''
|-
| style="text-align:left;" | Gel Isolation || $93.75 || $105.00 || per gel (5 lanes) || TBA ||
|-
| colspan="6" style="text-align:left;" | '''PLATE READER'''
|-
| style="text-align:left;" | Varioskan || $23.00 || $27.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || style="text-align:left;" | Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="6" style="text-align:left;" | '''ROBOTICS'''
|-
| style="text-align:left;" | Tecan EVO 150 || $93.75 || $105.00 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| style="text-align:left;" | non-filter tips || $10.00 || $11.20 || per box || || style="text-align:left;" | ** Please let us know if there is additional plasticware you would like us to stock.
|-
| style="text-align:left;" | Filter tips || $15.00 || $16.80 || per box || ||
|-
| style="text-align:left;" | Training || $81.25 || $91.00 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]] ||
|-
| colspan="6" style="text-align:left;" | '''NANODROP'''
|-
| style="text-align:left;" | Nanodrop || -- || -- || || Walk up service || style="text-align:left;" | * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable" style="width:100%; text-align:center;"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| style="text-align:left;" | Informatics Support || per hour || $180.00 || $205.00 || style="text-align:left;" | Very limited support for external users
|-
| style="text-align:left;" | Ingenuity Pathway Analysis || per dataset || N/A || $150.00 || style="text-align:left;" | Recovery of licence cost.
|}

BioMicroCenter:Pricing

2026-04-16T19:54:28Z

Cstjohn: /* COMPUTATIONAL SERVICES */

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,375.00 || $2,660.00
|-
| || One lane (~400M reads) || $1,250.00 || $1,400.00
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,312.50 || $3,710.00
|-
| || One lane (~400M reads) || $1,719.00 || $1,925.00
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,375.00 || $4,900.00
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 50 PE: F3 Lane (~100M reads) || $375.00 || $420.00
|-
| Rapid 300nt: F3 flowcell* || $2,250.00 || $2,520.00
|-
| 150 PE: F3 Lane (~100M reads) || $562.50 || $630.00
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit
|-
! Length !! Reads !! !!
|-
| 300nt || 5M || $893.75 || $1,001.00 || rowspan="9" | per flowcell
|-
| 600nt || 5M || $1,300.00 || $1,456.00
|-
| 100nt || 25M || $1,156.25 || $1,295.00
|-
| 300nt || 25M || $1,667.50 || $1,867.60
|-
| 600nt || 25M || $1,962.50 || $2,198.00
|-
| '''1000nt''' || 25M || $3,237.50 || $3,626.00
|-
| 100nt || 100M || $1,925.00 || $2,156.00
|-
| 300nt || 100M || $2,656.00 || $2,975.00
|-
| 600nt || 50M || $2,656.00 || $2,975.00
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,650.00 || $1,848.00 || rowspan="6" | per flowcell
|-
| 150nt V3 kits (eg. 75PE) || $1,787.50 || $2,002.00
|-
| 300nt V2 kit (eg. 150PE) || $1,993.75 || $2,233.00
|-
| 500nt V2 kit (eg. 250PE) || $2,200.00 || $2,464.00
|-
| 600nt V3 kit (eg. 300PE) || $2,750.00 || $3,080.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $962.50 || $1,078.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $43.75 || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $18.75 || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $31.25 || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $500.00 || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| 72 hour Flowcell || $1,500.00 || $1,680.00 || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| 72 hour Flowcell || colspan="2" | pricing in ilabs || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $37.50 || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $125.00 || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $136.50 || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $1,125.00 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $136.50 || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $204.00 || $235.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,125.00 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00
|-
| 16S Amplicon || HTL || 48 || $2,375.00 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $812.50 || $910.00
|-
| Other Amplicon || HTL || 48 || $2,000.00 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || N/A || $945.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $196.25 || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $293.75 || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $340.60 || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $273.00 || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $375.70 || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,875.00 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,750.00 || $4,200.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,312.50 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,625.00 || $2,940.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,125.00 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,500.00 || $2,800.00
|-
| || || 96 || $4,000.00 || $4,480.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,750.00 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Standard Ligation || LSK || $200.00 || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $65.00 || $70.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $375.00 || $450.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $188.00 || $225.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $340.60 || $393.00
|-
| Direct RNA || RNA004 || $200.00 || $240.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| DNA || 96 || $875.00 || $980.00 ||
|-
| RNA || 96 || $1,000.00 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $375.00 || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,250.00 || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,268.75 || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,188.00 || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,437.50 || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,881.00 || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,375.00 || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $337.50 || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $4,875.00 || $5,460.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $587.50 || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $56.00 || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $1,100.00 || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,094.00 || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $2,250.00 || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,875.00 || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $250.00 || $280.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Test Array || slide || $1,217.00 || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $7,510.00 || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $5,688.00 || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $9,813.00 || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $75.00 || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $60.00 || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| Slide Only || $188.00 || $210.00
|-
| Optimization Only || $1,000.00 || $1,120.00
|-
| Teton Panel || $9,000.00 || $10,080.00
|-
| Atlas Short || $6,875.00 || $7,700.00
|-
| Antibody Add-on || $450.00 || $504.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $18.75 || $21.00
|-
| Fragment Analyzer: Preloaded row || per row (12) || $100.00 || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $37.50 || $42.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $200.00 || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $62.50 || $70.00
|-
| qPCR only (Short read quantification test) || per sample || $31.25 || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $500.00 || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $60.00 || $66.00
|-
| FFPE Curl || per curl || $75.00 || $84.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Dr.Oligo || Assisted || $2,500.00 +consumables || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,250.00 +consumables || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,000.00 || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,250.00 || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $625.00 || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,875.00 || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $144.00 || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $375.00 || $420.00 ||
|-
| || add Quench || $125.00 || $140.00 ||
|}

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! SIGN UP !! Notes
|-
| colspan="6" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $25.00 || $28.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="6" | '''PIPPIN PREP'''
|-
| Gel Isolation || $93.75 || $105.00 || per gel (5 lanes) || TBA
|-
| Training || N/A || $50.00 || per session || TBA || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="6" | '''PLATE READER'''
|-
| Varioskan || $23.00 || $27.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="6" | '''ROBOTICS'''
|-
| Tecan EVO 150 || $93.75 || $105.00 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]]
|-
| non-filter tips || $10.00 || $11.20 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $15.00 || $16.80 || per box ||
|-
| Training || $81.25 || $91.00 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]]
|-
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Informatics Support || per hour || $180.00 || $205.00 || Very limited support for external users
|-
| Ingenuity Pathway Analysis || per dataset || N/A || $150.00 || Recovery of licence cost.
|-
|}

BioMicroCenter:Pricing

2026-04-16T19:53:53Z

Cstjohn: /* OTHER LAB SERVICES */

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,375.00 || $2,660.00
|-
| || One lane (~400M reads) || $1,250.00 || $1,400.00
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,312.50 || $3,710.00
|-
| || One lane (~400M reads) || $1,719.00 || $1,925.00
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,375.00 || $4,900.00
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 50 PE: F3 Lane (~100M reads) || $375.00 || $420.00
|-
| Rapid 300nt: F3 flowcell* || $2,250.00 || $2,520.00
|-
| 150 PE: F3 Lane (~100M reads) || $562.50 || $630.00
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit
|-
! Length !! Reads !! !!
|-
| 300nt || 5M || $893.75 || $1,001.00 || rowspan="9" | per flowcell
|-
| 600nt || 5M || $1,300.00 || $1,456.00
|-
| 100nt || 25M || $1,156.25 || $1,295.00
|-
| 300nt || 25M || $1,667.50 || $1,867.60
|-
| 600nt || 25M || $1,962.50 || $2,198.00
|-
| '''1000nt''' || 25M || $3,237.50 || $3,626.00
|-
| 100nt || 100M || $1,925.00 || $2,156.00
|-
| 300nt || 100M || $2,656.00 || $2,975.00
|-
| 600nt || 50M || $2,656.00 || $2,975.00
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,650.00 || $1,848.00 || rowspan="6" | per flowcell
|-
| 150nt V3 kits (eg. 75PE) || $1,787.50 || $2,002.00
|-
| 300nt V2 kit (eg. 150PE) || $1,993.75 || $2,233.00
|-
| 500nt V2 kit (eg. 250PE) || $2,200.00 || $2,464.00
|-
| 600nt V3 kit (eg. 300PE) || $2,750.00 || $3,080.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $962.50 || $1,078.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $43.75 || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $18.75 || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $31.25 || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $500.00 || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| 72 hour Flowcell || $1,500.00 || $1,680.00 || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| 72 hour Flowcell || colspan="2" | pricing in ilabs || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $37.50 || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $125.00 || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $136.50 || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $1,125.00 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $136.50 || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $204.00 || $235.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,125.00 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00
|-
| 16S Amplicon || HTL || 48 || $2,375.00 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $812.50 || $910.00
|-
| Other Amplicon || HTL || 48 || $2,000.00 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || N/A || $945.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $196.25 || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $293.75 || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $340.60 || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $273.00 || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $375.70 || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,875.00 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,750.00 || $4,200.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,312.50 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,625.00 || $2,940.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,125.00 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,500.00 || $2,800.00
|-
| || || 96 || $4,000.00 || $4,480.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,750.00 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Standard Ligation || LSK || $200.00 || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $65.00 || $70.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $375.00 || $450.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $188.00 || $225.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $340.60 || $393.00
|-
| Direct RNA || RNA004 || $200.00 || $240.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| DNA || 96 || $875.00 || $980.00 ||
|-
| RNA || 96 || $1,000.00 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $375.00 || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,250.00 || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,268.75 || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,188.00 || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,437.50 || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,881.00 || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,375.00 || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $337.50 || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $4,875.00 || $5,460.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $587.50 || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $56.00 || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $1,100.00 || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,094.00 || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $2,250.00 || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,875.00 || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $250.00 || $280.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Test Array || slide || $1,217.00 || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $7,510.00 || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $5,688.00 || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $9,813.00 || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $75.00 || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $60.00 || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| Slide Only || $188.00 || $210.00
|-
| Optimization Only || $1,000.00 || $1,120.00
|-
| Teton Panel || $9,000.00 || $10,080.00
|-
| Atlas Short || $6,875.00 || $7,700.00
|-
| Antibody Add-on || $450.00 || $504.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $18.75 || $21.00
|-
| Fragment Analyzer: Preloaded row || per row (12) || $100.00 || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $37.50 || $42.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $200.00 || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $62.50 || $70.00
|-
| qPCR only (Short read quantification test) || per sample || $31.25 || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $500.00 || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $60.00 || $66.00
|-
| FFPE Curl || per curl || $75.00 || $84.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Dr.Oligo || Assisted || $2,500.00 +consumables || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,250.00 +consumables || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,000.00 || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,250.00 || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $625.00 || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,875.00 || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $144.00 || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $375.00 || $420.00 ||
|-
| || add Quench || $125.00 || $140.00 ||
|}

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! SIGN UP !! Notes
|-
| colspan="6" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $25.00 || $28.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="6" | '''PIPPIN PREP'''
|-
| Gel Isolation || $93.75 || $105.00 || per gel (5 lanes) || TBA
|-
| Training || N/A || $50.00 || per session || TBA || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="6" | '''PLATE READER'''
|-
| Varioskan || $23.00 || $27.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="6" | '''ROBOTICS'''
|-
| Tecan EVO 150 || $93.75 || $105.00 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]]
|-
| non-filter tips || $10.00 || $11.20 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $15.00 || $16.80 || per box ||
|-
| Training || $81.25 || $91.00 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]]
|-
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Informatics Support || per hour || $180.00 || $205.00 || Very limited support for external users
|-
| Informatics Share || per 0.1FTE || N/A || N/A || MIT only
|-
| Ingenuity Pathway Analysis || per dataset || N/A || $150.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || N/A || N/A ||
|-
| Tape Archive / Recovery || per tape || N/A || N/A ||
|-
| Informatics Training Classes || per session || N/A || N/A ||
|}

BioMicroCenter:Pricing

2026-04-16T19:53:38Z

Cstjohn: /* OTHER LAB SERVICES */

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,375.00 || $2,660.00
|-
| || One lane (~400M reads) || $1,250.00 || $1,400.00
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,312.50 || $3,710.00
|-
| || One lane (~400M reads) || $1,719.00 || $1,925.00
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,375.00 || $4,900.00
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 50 PE: F3 Lane (~100M reads) || $375.00 || $420.00
|-
| Rapid 300nt: F3 flowcell* || $2,250.00 || $2,520.00
|-
| 150 PE: F3 Lane (~100M reads) || $562.50 || $630.00
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit
|-
! Length !! Reads !! !!
|-
| 300nt || 5M || $893.75 || $1,001.00 || rowspan="9" | per flowcell
|-
| 600nt || 5M || $1,300.00 || $1,456.00
|-
| 100nt || 25M || $1,156.25 || $1,295.00
|-
| 300nt || 25M || $1,667.50 || $1,867.60
|-
| 600nt || 25M || $1,962.50 || $2,198.00
|-
| '''1000nt''' || 25M || $3,237.50 || $3,626.00
|-
| 100nt || 100M || $1,925.00 || $2,156.00
|-
| 300nt || 100M || $2,656.00 || $2,975.00
|-
| 600nt || 50M || $2,656.00 || $2,975.00
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,650.00 || $1,848.00 || rowspan="6" | per flowcell
|-
| 150nt V3 kits (eg. 75PE) || $1,787.50 || $2,002.00
|-
| 300nt V2 kit (eg. 150PE) || $1,993.75 || $2,233.00
|-
| 500nt V2 kit (eg. 250PE) || $2,200.00 || $2,464.00
|-
| 600nt V3 kit (eg. 300PE) || $2,750.00 || $3,080.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $962.50 || $1,078.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $43.75 || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $18.75 || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $31.25 || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $500.00 || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| 72 hour Flowcell || $1,500.00 || $1,680.00 || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| 72 hour Flowcell || colspan="2" | pricing in ilabs || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $37.50 || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $125.00 || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $136.50 || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $1,125.00 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $136.50 || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $204.00 || $235.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,125.00 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00
|-
| 16S Amplicon || HTL || 48 || $2,375.00 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $812.50 || $910.00
|-
| Other Amplicon || HTL || 48 || $2,000.00 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || N/A || $945.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $196.25 || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $293.75 || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $340.60 || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $273.00 || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $375.70 || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,875.00 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,750.00 || $4,200.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,312.50 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,625.00 || $2,940.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,125.00 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,500.00 || $2,800.00
|-
| || || 96 || $4,000.00 || $4,480.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,750.00 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Standard Ligation || LSK || $200.00 || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $65.00 || $70.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $375.00 || $450.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $188.00 || $225.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $340.60 || $393.00
|-
| Direct RNA || RNA004 || $200.00 || $240.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| DNA || 96 || $875.00 || $980.00 ||
|-
| RNA || 96 || $1,000.00 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $375.00 || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,250.00 || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,268.75 || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,188.00 || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,437.50 || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,881.00 || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,375.00 || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $337.50 || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $4,875.00 || $5,460.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $587.50 || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $56.00 || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $1,100.00 || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,094.00 || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $2,250.00 || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,875.00 || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $250.00 || $280.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Test Array || slide || $1,217.00 || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $7,510.00 || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $5,688.00 || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $9,813.00 || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $75.00 || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $60.00 || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| Slide Only || $188.00 || $210.00
|-
| Optimization Only || $1,000.00 || $1,120.00
|-
| Teton Panel || $9,000.00 || $10,080.00
|-
| Atlas Short || $6,875.00 || $7,700.00
|-
| Antibody Add-on || $450.00 || $504.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $18.75 || $21.00
|-
| Fragment Analyzer: Preloaded row || per row (12) || $100.00 || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $37.50 || $42.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $200.00 || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $62.50 || $70.00
|-
| qPCR only (Short read quantification test) || per sample || $31.25 || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $500.00 || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $60.00 || $66.00
|-
| FFPE Curl || per curl || $75.00 || $84.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Dr.Oligo || Assisted || $2,500.00 +consumables || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,250.00 +consumables || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,000.00 || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,250.00 || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $625.00 || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,875.00 || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $144.00 || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $375.00 || $420.00 ||
|-
| || add Quench || $125.00 || $140.00 ||
|}

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! SIGN UP !! Notes
|-
| colspan="6" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $25.00 || $28.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="6" | '''PIPPIN PREP'''
|-
| Gel Isolation || $93.75 || $105.00 || per gel (5 lanes) || TBA
|-
| Training || N/A || $50.00 || per session || TBA || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="6" | '''PLATE READER'''
|-
| Varioskan || $23.00 || $27.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="6" | '''ROBOTICS'''
|-
| Tecan EVO 150 || $93.75 || $105.00 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]]
|-
| non-filter tips || $10.00 || $11.20 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $15.00 || $16.80 || per box ||
|-
| Training || $81.25 || $91.00 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]]
|-
| colspan="6" | '''NANODROP'''
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Informatics Support || per hour || $180.00 || $205.00 || Very limited support for external users
|-
| Informatics Share || per 0.1FTE || N/A || N/A || MIT only
|-
| Ingenuity Pathway Analysis || per dataset || N/A || $150.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || N/A || N/A ||
|-
| Tape Archive / Recovery || per tape || N/A || N/A ||
|-
| Informatics Training Classes || per session || N/A || N/A ||
|}

BioMicroCenter:Pricing

2026-04-16T19:53:02Z

Cstjohn: /* OTHER LAB SERVICES */

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,375.00 || $2,660.00
|-
| || One lane (~400M reads) || $1,250.00 || $1,400.00
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,312.50 || $3,710.00
|-
| || One lane (~400M reads) || $1,719.00 || $1,925.00
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,375.00 || $4,900.00
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 50 PE: F3 Lane (~100M reads) || $375.00 || $420.00
|-
| Rapid 300nt: F3 flowcell* || $2,250.00 || $2,520.00
|-
| 150 PE: F3 Lane (~100M reads) || $562.50 || $630.00
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit
|-
! Length !! Reads !! !!
|-
| 300nt || 5M || $893.75 || $1,001.00 || rowspan="9" | per flowcell
|-
| 600nt || 5M || $1,300.00 || $1,456.00
|-
| 100nt || 25M || $1,156.25 || $1,295.00
|-
| 300nt || 25M || $1,667.50 || $1,867.60
|-
| 600nt || 25M || $1,962.50 || $2,198.00
|-
| '''1000nt''' || 25M || $3,237.50 || $3,626.00
|-
| 100nt || 100M || $1,925.00 || $2,156.00
|-
| 300nt || 100M || $2,656.00 || $2,975.00
|-
| 600nt || 50M || $2,656.00 || $2,975.00
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,650.00 || $1,848.00 || rowspan="6" | per flowcell
|-
| 150nt V3 kits (eg. 75PE) || $1,787.50 || $2,002.00
|-
| 300nt V2 kit (eg. 150PE) || $1,993.75 || $2,233.00
|-
| 500nt V2 kit (eg. 250PE) || $2,200.00 || $2,464.00
|-
| 600nt V3 kit (eg. 300PE) || $2,750.00 || $3,080.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $962.50 || $1,078.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $43.75 || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $18.75 || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $31.25 || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $500.00 || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| 72 hour Flowcell || $1,500.00 || $1,680.00 || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| 72 hour Flowcell || colspan="2" | pricing in ilabs || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $37.50 || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $125.00 || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $136.50 || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $1,125.00 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $136.50 || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $204.00 || $235.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,125.00 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00
|-
| 16S Amplicon || HTL || 48 || $2,375.00 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $812.50 || $910.00
|-
| Other Amplicon || HTL || 48 || $2,000.00 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || N/A || $945.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $196.25 || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $293.75 || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $340.60 || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $273.00 || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $375.70 || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,875.00 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,750.00 || $4,200.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,312.50 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,625.00 || $2,940.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,125.00 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,500.00 || $2,800.00
|-
| || || 96 || $4,000.00 || $4,480.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,750.00 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Standard Ligation || LSK || $200.00 || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $65.00 || $70.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $375.00 || $450.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $188.00 || $225.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $340.60 || $393.00
|-
| Direct RNA || RNA004 || $200.00 || $240.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| DNA || 96 || $875.00 || $980.00 ||
|-
| RNA || 96 || $1,000.00 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $375.00 || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,250.00 || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,268.75 || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,188.00 || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,437.50 || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,881.00 || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,375.00 || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $337.50 || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $4,875.00 || $5,460.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $587.50 || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $56.00 || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $1,100.00 || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,094.00 || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $2,250.00 || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,875.00 || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $250.00 || $280.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Test Array || slide || $1,217.00 || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $7,510.00 || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $5,688.00 || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $9,813.00 || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $75.00 || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $60.00 || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| Slide Only || $188.00 || $210.00
|-
| Optimization Only || $1,000.00 || $1,120.00
|-
| Teton Panel || $9,000.00 || $10,080.00
|-
| Atlas Short || $6,875.00 || $7,700.00
|-
| Antibody Add-on || $450.00 || $504.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $18.75 || $21.00
|-
| Fragment Analyzer: Preloaded row || per row (12) || $100.00 || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $37.50 || $42.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $200.00 || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $62.50 || $70.00
|-
| qPCR only (Short read quantification test) || per sample || $31.25 || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $500.00 || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $60.00 || $66.00
|-
| FFPE Curl || per curl || $75.00 || $84.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Dr.Oligo || Assisted || $2,500.00 +consumables || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,250.00 +consumables || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,000.00 || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,250.00 || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $625.00 || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,875.00 || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $144.00 || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $375.00 || $420.00 ||
|-
| || add Quench || $125.00 || $140.00 ||
|}

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! SIGN UP !! Notes
|-
| colspan="6" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $25.00 || $28.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="6" | '''PIPPIN PREP'''
|-
| Gel Isolation || $93.75 || $105.00 || per gel (5 lanes) || TBA
|-
| Training || N/A || $50.00 || per session || TBA || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="6" | '''PLATE READER'''
|-
| Varioskan || $23.00 || $27.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="6" | '''ROBOTICS'''
|-
| Tecan EVO 150 || $93.75 || $105.00 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]]
|-
| non-filter tips || $10.00 || $11.20 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $15.00 || $16.80 || per box ||
|-
| Training || $81.25 || $91.00 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]]
|-
| colspan="6" | '''NANODROP'''
|-
| Nanodrop || -- || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Informatics Support || per hour || $180.00 || $205.00 || Very limited support for external users
|-
| Informatics Share || per 0.1FTE || N/A || N/A || MIT only
|-
| Ingenuity Pathway Analysis || per dataset || N/A || $150.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || N/A || N/A ||
|-
| Tape Archive / Recovery || per tape || N/A || N/A ||
|-
| Informatics Training Classes || per session || N/A || N/A ||
|}

BioMicroCenter:Pricing

2026-04-16T19:52:00Z

Cstjohn: /* REAL TIME PCR */

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,375.00 || $2,660.00
|-
| || One lane (~400M reads) || $1,250.00 || $1,400.00
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,312.50 || $3,710.00
|-
| || One lane (~400M reads) || $1,719.00 || $1,925.00
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,375.00 || $4,900.00
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 50 PE: F3 Lane (~100M reads) || $375.00 || $420.00
|-
| Rapid 300nt: F3 flowcell* || $2,250.00 || $2,520.00
|-
| 150 PE: F3 Lane (~100M reads) || $562.50 || $630.00
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit
|-
! Length !! Reads !! !!
|-
| 300nt || 5M || $893.75 || $1,001.00 || rowspan="9" | per flowcell
|-
| 600nt || 5M || $1,300.00 || $1,456.00
|-
| 100nt || 25M || $1,156.25 || $1,295.00
|-
| 300nt || 25M || $1,667.50 || $1,867.60
|-
| 600nt || 25M || $1,962.50 || $2,198.00
|-
| '''1000nt''' || 25M || $3,237.50 || $3,626.00
|-
| 100nt || 100M || $1,925.00 || $2,156.00
|-
| 300nt || 100M || $2,656.00 || $2,975.00
|-
| 600nt || 50M || $2,656.00 || $2,975.00
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,650.00 || $1,848.00 || rowspan="6" | per flowcell
|-
| 150nt V3 kits (eg. 75PE) || $1,787.50 || $2,002.00
|-
| 300nt V2 kit (eg. 150PE) || $1,993.75 || $2,233.00
|-
| 500nt V2 kit (eg. 250PE) || $2,200.00 || $2,464.00
|-
| 600nt V3 kit (eg. 300PE) || $2,750.00 || $3,080.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $962.50 || $1,078.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $43.75 || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $18.75 || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $31.25 || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $500.00 || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| 72 hour Flowcell || $1,500.00 || $1,680.00 || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| 72 hour Flowcell || colspan="2" | pricing in ilabs || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $37.50 || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $125.00 || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $136.50 || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $1,125.00 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $136.50 || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $204.00 || $235.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,125.00 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00
|-
| 16S Amplicon || HTL || 48 || $2,375.00 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $812.50 || $910.00
|-
| Other Amplicon || HTL || 48 || $2,000.00 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || N/A || $945.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $196.25 || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $293.75 || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $340.60 || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $273.00 || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $375.70 || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,875.00 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,750.00 || $4,200.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,312.50 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,625.00 || $2,940.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,125.00 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,500.00 || $2,800.00
|-
| || || 96 || $4,000.00 || $4,480.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,750.00 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Standard Ligation || LSK || $200.00 || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $65.00 || $70.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $375.00 || $450.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $188.00 || $225.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $340.60 || $393.00
|-
| Direct RNA || RNA004 || $200.00 || $240.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| DNA || 96 || $875.00 || $980.00 ||
|-
| RNA || 96 || $1,000.00 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $375.00 || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,250.00 || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,268.75 || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,188.00 || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,437.50 || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,881.00 || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,375.00 || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $337.50 || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $4,875.00 || $5,460.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $587.50 || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $56.00 || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $1,100.00 || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,094.00 || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $2,250.00 || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,875.00 || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $250.00 || $280.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Test Array || slide || $1,217.00 || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $7,510.00 || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $5,688.00 || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $9,813.00 || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $75.00 || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $60.00 || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| Slide Only || $188.00 || $210.00
|-
| Optimization Only || $1,000.00 || $1,120.00
|-
| Teton Panel || $9,000.00 || $10,080.00
|-
| Atlas Short || $6,875.00 || $7,700.00
|-
| Antibody Add-on || $450.00 || $504.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $18.75 || $21.00
|-
| Fragment Analyzer: Preloaded row || per row (12) || $100.00 || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $37.50 || $42.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $200.00 || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $62.50 || $70.00
|-
| qPCR only (Short read quantification test) || per sample || $31.25 || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $500.00 || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $60.00 || $66.00
|-
| FFPE Curl || per curl || $75.00 || $84.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Dr.Oligo || Assisted || $2,500.00 +consumables || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,250.00 +consumables || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,000.00 || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,250.00 || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $625.00 || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,875.00 || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $144.00 || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $375.00 || $420.00 ||
|-
| || add Quench || $125.00 || $140.00 ||
|}

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! SIGN UP !! Notes
|-
| colspan="6" | '''COVARIS SONICATOR''' ||
|-
| COVARIS E220 || $25.00 || $28.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="6" | '''PIPPIN PREP''' ||
|-
| Gel Isolation || $93.75 || $105.00 || per gel (5 lanes) || TBA ||
|-
| Training || N/A || $50.00 || per session || TBA || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="6" | '''PLATE READER''' ||
|-
| Varioskan || $23.00 || $27.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="6" | '''ROBOTICS''' ||
|-
| Tecan EVO 150 || $93.75 || $105.00 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| non-filter tips || $10.00 || $11.20 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $15.00 || $16.80 || per box || ||
|-
| Training || $81.25 || $91.00 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]] ||
|-
| colspan="6" | '''NANODROP''' ||
|-
| Nanodrop || -- || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Informatics Support || per hour || $180.00 || $205.00 || Very limited support for external users
|-
| Informatics Share || per 0.1FTE || N/A || N/A || MIT only
|-
| Ingenuity Pathway Analysis || per dataset || N/A || $150.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || N/A || N/A ||
|-
| Tape Archive / Recovery || per tape || N/A || N/A ||
|-
| Informatics Training Classes || per session || N/A || N/A ||
|}

BioMicroCenter:Pricing

2026-04-16T19:51:38Z

Cstjohn: /* QUALITY CONTROL */

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,375.00 || $2,660.00
|-
| || One lane (~400M reads) || $1,250.00 || $1,400.00
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,312.50 || $3,710.00
|-
| || One lane (~400M reads) || $1,719.00 || $1,925.00
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,375.00 || $4,900.00
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 50 PE: F3 Lane (~100M reads) || $375.00 || $420.00
|-
| Rapid 300nt: F3 flowcell* || $2,250.00 || $2,520.00
|-
| 150 PE: F3 Lane (~100M reads) || $562.50 || $630.00
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit
|-
! Length !! Reads !! !!
|-
| 300nt || 5M || $893.75 || $1,001.00 || rowspan="9" | per flowcell
|-
| 600nt || 5M || $1,300.00 || $1,456.00
|-
| 100nt || 25M || $1,156.25 || $1,295.00
|-
| 300nt || 25M || $1,667.50 || $1,867.60
|-
| 600nt || 25M || $1,962.50 || $2,198.00
|-
| '''1000nt''' || 25M || $3,237.50 || $3,626.00
|-
| 100nt || 100M || $1,925.00 || $2,156.00
|-
| 300nt || 100M || $2,656.00 || $2,975.00
|-
| 600nt || 50M || $2,656.00 || $2,975.00
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,650.00 || $1,848.00 || rowspan="6" | per flowcell
|-
| 150nt V3 kits (eg. 75PE) || $1,787.50 || $2,002.00
|-
| 300nt V2 kit (eg. 150PE) || $1,993.75 || $2,233.00
|-
| 500nt V2 kit (eg. 250PE) || $2,200.00 || $2,464.00
|-
| 600nt V3 kit (eg. 300PE) || $2,750.00 || $3,080.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $962.50 || $1,078.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $43.75 || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $18.75 || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $31.25 || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $500.00 || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| 72 hour Flowcell || $1,500.00 || $1,680.00 || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| 72 hour Flowcell || colspan="2" | pricing in ilabs || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $37.50 || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $125.00 || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $136.50 || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $1,125.00 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $136.50 || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $204.00 || $235.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,125.00 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00
|-
| 16S Amplicon || HTL || 48 || $2,375.00 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $812.50 || $910.00
|-
| Other Amplicon || HTL || 48 || $2,000.00 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || N/A || $945.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $196.25 || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $293.75 || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $340.60 || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $273.00 || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $375.70 || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,875.00 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,750.00 || $4,200.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,312.50 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,625.00 || $2,940.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,125.00 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,500.00 || $2,800.00
|-
| || || 96 || $4,000.00 || $4,480.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,750.00 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Standard Ligation || LSK || $200.00 || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $65.00 || $70.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $375.00 || $450.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $188.00 || $225.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $340.60 || $393.00
|-
| Direct RNA || RNA004 || $200.00 || $240.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| DNA || 96 || $875.00 || $980.00 ||
|-
| RNA || 96 || $1,000.00 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $375.00 || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,250.00 || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,268.75 || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,188.00 || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,437.50 || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,881.00 || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,375.00 || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $337.50 || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $4,875.00 || $5,460.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $587.50 || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $56.00 || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $1,100.00 || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,094.00 || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $2,250.00 || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,875.00 || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $250.00 || $280.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Test Array || slide || $1,217.00 || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $7,510.00 || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $5,688.00 || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $9,813.00 || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $75.00 || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $60.00 || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| Slide Only || $188.00 || $210.00
|-
| Optimization Only || $1,000.00 || $1,120.00
|-
| Teton Panel || $9,000.00 || $10,080.00
|-
| Atlas Short || $6,875.00 || $7,700.00
|-
| Antibody Add-on || $450.00 || $504.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $18.75 || $21.00
|-
| Fragment Analyzer: Preloaded row || per row (12) || $100.00 || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $37.50 || $42.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $200.00 || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $62.50 || $70.00
|-
| qPCR only (Short read quantification test) || per sample || $31.25 || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $500.00 || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $60.00 || $66.00
|-
| FFPE Curl || per curl || $75.00 || $84.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Dr.Oligo || Assisted || $2,500.00 +consumables || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,250.00 +consumables || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,000.00 || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,250.00 || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $625.00 || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,875.00 || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $144.00 || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $375.00 || $420.00 ||
|-
| || add Quench || $125.00 || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to external users.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! SIGN UP !! Notes
|-
| colspan="6" | '''COVARIS SONICATOR''' ||
|-
| COVARIS E220 || $25.00 || $28.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="6" | '''PIPPIN PREP''' ||
|-
| Gel Isolation || $93.75 || $105.00 || per gel (5 lanes) || TBA ||
|-
| Training || N/A || $50.00 || per session || TBA || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="6" | '''PLATE READER''' ||
|-
| Varioskan || $23.00 || $27.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="6" | '''ROBOTICS''' ||
|-
| Tecan EVO 150 || $93.75 || $105.00 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| non-filter tips || $10.00 || $11.20 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $15.00 || $16.80 || per box || ||
|-
| Training || $81.25 || $91.00 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]] ||
|-
| colspan="6" | '''NANODROP''' ||
|-
| Nanodrop || -- || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Informatics Support || per hour || $180.00 || $205.00 || Very limited support for external users
|-
| Informatics Share || per 0.1FTE || N/A || N/A || MIT only
|-
| Ingenuity Pathway Analysis || per dataset || N/A || $150.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || N/A || N/A ||
|-
| Tape Archive / Recovery || per tape || N/A || N/A ||
|-
| Informatics Training Classes || per session || N/A || N/A ||
|}

BioMicroCenter:Pricing

2026-04-16T19:51:05Z

Cstjohn: /* AVITI24 */

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,375.00 || $2,660.00
|-
| || One lane (~400M reads) || $1,250.00 || $1,400.00
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,312.50 || $3,710.00
|-
| || One lane (~400M reads) || $1,719.00 || $1,925.00
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,375.00 || $4,900.00
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 50 PE: F3 Lane (~100M reads) || $375.00 || $420.00
|-
| Rapid 300nt: F3 flowcell* || $2,250.00 || $2,520.00
|-
| 150 PE: F3 Lane (~100M reads) || $562.50 || $630.00
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit
|-
! Length !! Reads !! !!
|-
| 300nt || 5M || $893.75 || $1,001.00 || rowspan="9" | per flowcell
|-
| 600nt || 5M || $1,300.00 || $1,456.00
|-
| 100nt || 25M || $1,156.25 || $1,295.00
|-
| 300nt || 25M || $1,667.50 || $1,867.60
|-
| 600nt || 25M || $1,962.50 || $2,198.00
|-
| '''1000nt''' || 25M || $3,237.50 || $3,626.00
|-
| 100nt || 100M || $1,925.00 || $2,156.00
|-
| 300nt || 100M || $2,656.00 || $2,975.00
|-
| 600nt || 50M || $2,656.00 || $2,975.00
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,650.00 || $1,848.00 || rowspan="6" | per flowcell
|-
| 150nt V3 kits (eg. 75PE) || $1,787.50 || $2,002.00
|-
| 300nt V2 kit (eg. 150PE) || $1,993.75 || $2,233.00
|-
| 500nt V2 kit (eg. 250PE) || $2,200.00 || $2,464.00
|-
| 600nt V3 kit (eg. 300PE) || $2,750.00 || $3,080.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $962.50 || $1,078.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $43.75 || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $18.75 || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $31.25 || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $500.00 || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| 72 hour Flowcell || $1,500.00 || $1,680.00 || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| 72 hour Flowcell || colspan="2" | pricing in ilabs || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $37.50 || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $125.00 || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $136.50 || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $1,125.00 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $136.50 || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $204.00 || $235.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,125.00 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00
|-
| 16S Amplicon || HTL || 48 || $2,375.00 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $812.50 || $910.00
|-
| Other Amplicon || HTL || 48 || $2,000.00 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || N/A || $945.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $196.25 || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $293.75 || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $340.60 || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $273.00 || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $375.70 || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,875.00 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,750.00 || $4,200.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,312.50 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,625.00 || $2,940.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,125.00 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,500.00 || $2,800.00
|-
| || || 96 || $4,000.00 || $4,480.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,750.00 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Standard Ligation || LSK || $200.00 || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $65.00 || $70.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $375.00 || $450.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $188.00 || $225.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $340.60 || $393.00
|-
| Direct RNA || RNA004 || $200.00 || $240.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| DNA || 96 || $875.00 || $980.00 ||
|-
| RNA || 96 || $1,000.00 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $375.00 || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,250.00 || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,268.75 || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,188.00 || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,437.50 || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,881.00 || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,375.00 || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $337.50 || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $4,875.00 || $5,460.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $587.50 || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $56.00 || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $1,100.00 || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,094.00 || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $2,250.00 || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,875.00 || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $250.00 || $280.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Test Array || slide || $1,217.00 || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $7,510.00 || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $5,688.00 || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $9,813.00 || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $75.00 || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $60.00 || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| Slide Only || $188.00 || $210.00
|-
| Optimization Only || $1,000.00 || $1,120.00
|-
| Teton Panel || $9,000.00 || $10,080.00
|-
| Atlas Short || $6,875.00 || $7,700.00
|-
| Antibody Add-on || $450.00 || $504.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $18.75 || $21.00 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $100.00 || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $37.50 || $42.00 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $200.00 || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $62.50 || $70.00 ||
|-
| qPCR only (Short read quantification test) || per sample || $31.25 || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $500.00 || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:''' ||
|-
| Fresh Frozen Curl || per curl || $60.00 || $66.00 ||
|-
| FFPE Curl || per curl || $75.00 || $84.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Dr.Oligo || Assisted || $2,500.00 +consumables || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,250.00 +consumables || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,000.00 || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,250.00 || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $625.00 || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,875.00 || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $144.00 || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $375.00 || $420.00 ||
|-
| || add Quench || $125.00 || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to external users.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! SIGN UP !! Notes
|-
| colspan="6" | '''COVARIS SONICATOR''' ||
|-
| COVARIS E220 || $25.00 || $28.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="6" | '''PIPPIN PREP''' ||
|-
| Gel Isolation || $93.75 || $105.00 || per gel (5 lanes) || TBA ||
|-
| Training || N/A || $50.00 || per session || TBA || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="6" | '''PLATE READER''' ||
|-
| Varioskan || $23.00 || $27.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="6" | '''ROBOTICS''' ||
|-
| Tecan EVO 150 || $93.75 || $105.00 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| non-filter tips || $10.00 || $11.20 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $15.00 || $16.80 || per box || ||
|-
| Training || $81.25 || $91.00 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]] ||
|-
| colspan="6" | '''NANODROP''' ||
|-
| Nanodrop || -- || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Informatics Support || per hour || $180.00 || $205.00 || Very limited support for external users
|-
| Informatics Share || per 0.1FTE || N/A || N/A || MIT only
|-
| Ingenuity Pathway Analysis || per dataset || N/A || $150.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || N/A || N/A ||
|-
| Tape Archive / Recovery || per tape || N/A || N/A ||
|-
| Informatics Training Classes || per session || N/A || N/A ||
|}

BioMicroCenter:Pricing

2026-04-16T19:50:56Z

Cstjohn: /* AVITI24 */

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,375.00 || $2,660.00
|-
| || One lane (~400M reads) || $1,250.00 || $1,400.00
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,312.50 || $3,710.00
|-
| || One lane (~400M reads) || $1,719.00 || $1,925.00
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,375.00 || $4,900.00
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 50 PE: F3 Lane (~100M reads) || $375.00 || $420.00
|-
| Rapid 300nt: F3 flowcell* || $2,250.00 || $2,520.00
|-
| 150 PE: F3 Lane (~100M reads) || $562.50 || $630.00
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit
|-
! Length !! Reads !! !!
|-
| 300nt || 5M || $893.75 || $1,001.00 || rowspan="9" | per flowcell
|-
| 600nt || 5M || $1,300.00 || $1,456.00
|-
| 100nt || 25M || $1,156.25 || $1,295.00
|-
| 300nt || 25M || $1,667.50 || $1,867.60
|-
| 600nt || 25M || $1,962.50 || $2,198.00
|-
| '''1000nt''' || 25M || $3,237.50 || $3,626.00
|-
| 100nt || 100M || $1,925.00 || $2,156.00
|-
| 300nt || 100M || $2,656.00 || $2,975.00
|-
| 600nt || 50M || $2,656.00 || $2,975.00
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,650.00 || $1,848.00 || rowspan="6" | per flowcell
|-
| 150nt V3 kits (eg. 75PE) || $1,787.50 || $2,002.00
|-
| 300nt V2 kit (eg. 150PE) || $1,993.75 || $2,233.00
|-
| 500nt V2 kit (eg. 250PE) || $2,200.00 || $2,464.00
|-
| 600nt V3 kit (eg. 300PE) || $2,750.00 || $3,080.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $962.50 || $1,078.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $43.75 || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $18.75 || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $31.25 || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $500.00 || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| 72 hour Flowcell || $1,500.00 || $1,680.00 || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| 72 hour Flowcell || colspan="2" | pricing in ilabs || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $37.50 || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $125.00 || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $136.50 || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $1,125.00 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $136.50 || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $204.00 || $235.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,125.00 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00
|-
| 16S Amplicon || HTL || 48 || $2,375.00 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $812.50 || $910.00
|-
| Other Amplicon || HTL || 48 || $2,000.00 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || N/A || $945.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $196.25 || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $293.75 || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $340.60 || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $273.00 || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $375.70 || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,875.00 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,750.00 || $4,200.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,312.50 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,625.00 || $2,940.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,125.00 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,500.00 || $2,800.00
|-
| || || 96 || $4,000.00 || $4,480.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,750.00 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Standard Ligation || LSK || $200.00 || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $65.00 || $70.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $375.00 || $450.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $188.00 || $225.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $340.60 || $393.00
|-
| Direct RNA || RNA004 || $200.00 || $240.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| DNA || 96 || $875.00 || $980.00 ||
|-
| RNA || 96 || $1,000.00 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $375.00 || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,250.00 || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,268.75 || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,188.00 || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,437.50 || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,881.00 || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,375.00 || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $337.50 || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $4,875.00 || $5,460.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $587.50 || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $56.00 || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $1,100.00 || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,094.00 || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $2,250.00 || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,875.00 || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $250.00 || $280.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Test Array || slide || $1,217.00 || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $7,510.00 || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $5,688.00 || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $9,813.00 || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $75.00 || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $60.00 || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Slide Only || $188.00 || $210.00
|-
| Optimization Only || $1,000.00 || $1,120.00
|-
| Teton Panel || $9,000.00 || $10,080.00
|-
| Atlas Short || $6,875.00 || $7,700.00
|-
| Antibody Add-on || $450.00 || $504.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $18.75 || $21.00 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $100.00 || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $37.50 || $42.00 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $200.00 || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $62.50 || $70.00 ||
|-
| qPCR only (Short read quantification test) || per sample || $31.25 || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $500.00 || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:''' ||
|-
| Fresh Frozen Curl || per curl || $60.00 || $66.00 ||
|-
| FFPE Curl || per curl || $75.00 || $84.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Dr.Oligo || Assisted || $2,500.00 +consumables || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,250.00 +consumables || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,000.00 || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,250.00 || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $625.00 || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,875.00 || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $144.00 || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $375.00 || $420.00 ||
|-
| || add Quench || $125.00 || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to external users.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! SIGN UP !! Notes
|-
| colspan="6" | '''COVARIS SONICATOR''' ||
|-
| COVARIS E220 || $25.00 || $28.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="6" | '''PIPPIN PREP''' ||
|-
| Gel Isolation || $93.75 || $105.00 || per gel (5 lanes) || TBA ||
|-
| Training || N/A || $50.00 || per session || TBA || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="6" | '''PLATE READER''' ||
|-
| Varioskan || $23.00 || $27.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="6" | '''ROBOTICS''' ||
|-
| Tecan EVO 150 || $93.75 || $105.00 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| non-filter tips || $10.00 || $11.20 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $15.00 || $16.80 || per box || ||
|-
| Training || $81.25 || $91.00 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]] ||
|-
| colspan="6" | '''NANODROP''' ||
|-
| Nanodrop || -- || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Informatics Support || per hour || $180.00 || $205.00 || Very limited support for external users
|-
| Informatics Share || per 0.1FTE || N/A || N/A || MIT only
|-
| Ingenuity Pathway Analysis || per dataset || N/A || $150.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || N/A || N/A ||
|-
| Tape Archive / Recovery || per tape || N/A || N/A ||
|-
| Informatics Training Classes || per session || N/A || N/A ||
|}

BioMicroCenter:Pricing

2026-04-16T19:50:06Z

Cstjohn: /* 10X CHROMIUM */

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,375.00 || $2,660.00
|-
| || One lane (~400M reads) || $1,250.00 || $1,400.00
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,312.50 || $3,710.00
|-
| || One lane (~400M reads) || $1,719.00 || $1,925.00
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,375.00 || $4,900.00
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 50 PE: F3 Lane (~100M reads) || $375.00 || $420.00
|-
| Rapid 300nt: F3 flowcell* || $2,250.00 || $2,520.00
|-
| 150 PE: F3 Lane (~100M reads) || $562.50 || $630.00
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit
|-
! Length !! Reads !! !!
|-
| 300nt || 5M || $893.75 || $1,001.00 || rowspan="9" | per flowcell
|-
| 600nt || 5M || $1,300.00 || $1,456.00
|-
| 100nt || 25M || $1,156.25 || $1,295.00
|-
| 300nt || 25M || $1,667.50 || $1,867.60
|-
| 600nt || 25M || $1,962.50 || $2,198.00
|-
| '''1000nt''' || 25M || $3,237.50 || $3,626.00
|-
| 100nt || 100M || $1,925.00 || $2,156.00
|-
| 300nt || 100M || $2,656.00 || $2,975.00
|-
| 600nt || 50M || $2,656.00 || $2,975.00
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,650.00 || $1,848.00 || rowspan="6" | per flowcell
|-
| 150nt V3 kits (eg. 75PE) || $1,787.50 || $2,002.00
|-
| 300nt V2 kit (eg. 150PE) || $1,993.75 || $2,233.00
|-
| 500nt V2 kit (eg. 250PE) || $2,200.00 || $2,464.00
|-
| 600nt V3 kit (eg. 300PE) || $2,750.00 || $3,080.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $962.50 || $1,078.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $43.75 || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $18.75 || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $31.25 || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $500.00 || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| 72 hour Flowcell || $1,500.00 || $1,680.00 || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| 72 hour Flowcell || colspan="2" | pricing in ilabs || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $37.50 || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $125.00 || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $136.50 || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $1,125.00 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $136.50 || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $204.00 || $235.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,125.00 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00
|-
| 16S Amplicon || HTL || 48 || $2,375.00 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $812.50 || $910.00
|-
| Other Amplicon || HTL || 48 || $2,000.00 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || N/A || $945.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $196.25 || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $293.75 || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $340.60 || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $273.00 || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $375.70 || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,875.00 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,750.00 || $4,200.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,312.50 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,625.00 || $2,940.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,125.00 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,500.00 || $2,800.00
|-
| || || 96 || $4,000.00 || $4,480.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,750.00 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Standard Ligation || LSK || $200.00 || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $65.00 || $70.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $375.00 || $450.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $188.00 || $225.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $340.60 || $393.00
|-
| Direct RNA || RNA004 || $200.00 || $240.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| DNA || 96 || $875.00 || $980.00 ||
|-
| RNA || 96 || $1,000.00 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $375.00 || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,250.00 || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="5" | '''Assisted Service'''
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,268.75 || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,188.00 || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,437.50 || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,881.00 || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,375.00 || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $337.50 || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $4,875.00 || $5,460.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $587.50 || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $56.00 || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq''
|-
| ATACseq - setup || setup || $1,100.00 || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,094.00 || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $2,250.00 || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,875.00 || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $250.00 || $280.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Test Array || slide || $1,217.00 || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $7,510.00 || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $5,688.00 || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $9,813.00 || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $75.00 || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $60.00 || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Slide Only || per slide || $188.00 || $210.00 ||
|-
| Optimization Only || per slide || $1,000.00 || $1,120.00 ||
|-
| Teton Panel || per slide || $9,000.00 || $10,080.00 ||
|-
| Atlas Short || per slide || $6,875.00 || $7,700.00 ||
|-
| Antibody Add-on || per slide || $450.00 || $504.00 ||
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $18.75 || $21.00 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $100.00 || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $37.50 || $42.00 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $200.00 || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $62.50 || $70.00 ||
|-
| qPCR only (Short read quantification test) || per sample || $31.25 || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $500.00 || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:''' ||
|-
| Fresh Frozen Curl || per curl || $60.00 || $66.00 ||
|-
| FFPE Curl || per curl || $75.00 || $84.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Dr.Oligo || Assisted || $2,500.00 +consumables || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,250.00 +consumables || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,000.00 || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,250.00 || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $625.00 || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,875.00 || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $144.00 || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $375.00 || $420.00 ||
|-
| || add Quench || $125.00 || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to external users.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! SIGN UP !! Notes
|-
| colspan="6" | '''COVARIS SONICATOR''' ||
|-
| COVARIS E220 || $25.00 || $28.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="6" | '''PIPPIN PREP''' ||
|-
| Gel Isolation || $93.75 || $105.00 || per gel (5 lanes) || TBA ||
|-
| Training || N/A || $50.00 || per session || TBA || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="6" | '''PLATE READER''' ||
|-
| Varioskan || $23.00 || $27.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="6" | '''ROBOTICS''' ||
|-
| Tecan EVO 150 || $93.75 || $105.00 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| non-filter tips || $10.00 || $11.20 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $15.00 || $16.80 || per box || ||
|-
| Training || $81.25 || $91.00 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]] ||
|-
| colspan="6" | '''NANODROP''' ||
|-
| Nanodrop || -- || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Informatics Support || per hour || $180.00 || $205.00 || Very limited support for external users
|-
| Informatics Share || per 0.1FTE || N/A || N/A || MIT only
|-
| Ingenuity Pathway Analysis || per dataset || N/A || $150.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || N/A || N/A ||
|-
| Tape Archive / Recovery || per tape || N/A || N/A ||
|-
| Informatics Training Classes || per session || N/A || N/A ||
|}

BioMicroCenter:Pricing

2026-04-16T19:49:30Z

Cstjohn: /* LONG READ LIBRARIES */

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,375.00 || $2,660.00
|-
| || One lane (~400M reads) || $1,250.00 || $1,400.00
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,312.50 || $3,710.00
|-
| || One lane (~400M reads) || $1,719.00 || $1,925.00
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,375.00 || $4,900.00
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 50 PE: F3 Lane (~100M reads) || $375.00 || $420.00
|-
| Rapid 300nt: F3 flowcell* || $2,250.00 || $2,520.00
|-
| 150 PE: F3 Lane (~100M reads) || $562.50 || $630.00
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit
|-
! Length !! Reads !! !!
|-
| 300nt || 5M || $893.75 || $1,001.00 || rowspan="9" | per flowcell
|-
| 600nt || 5M || $1,300.00 || $1,456.00
|-
| 100nt || 25M || $1,156.25 || $1,295.00
|-
| 300nt || 25M || $1,667.50 || $1,867.60
|-
| 600nt || 25M || $1,962.50 || $2,198.00
|-
| '''1000nt''' || 25M || $3,237.50 || $3,626.00
|-
| 100nt || 100M || $1,925.00 || $2,156.00
|-
| 300nt || 100M || $2,656.00 || $2,975.00
|-
| 600nt || 50M || $2,656.00 || $2,975.00
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,650.00 || $1,848.00 || rowspan="6" | per flowcell
|-
| 150nt V3 kits (eg. 75PE) || $1,787.50 || $2,002.00
|-
| 300nt V2 kit (eg. 150PE) || $1,993.75 || $2,233.00
|-
| 500nt V2 kit (eg. 250PE) || $2,200.00 || $2,464.00
|-
| 600nt V3 kit (eg. 300PE) || $2,750.00 || $3,080.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $962.50 || $1,078.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $43.75 || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $18.75 || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $31.25 || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $500.00 || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| 72 hour Flowcell || $1,500.00 || $1,680.00 || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| 72 hour Flowcell || colspan="2" | pricing in ilabs || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $37.50 || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $125.00 || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $136.50 || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $1,125.00 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $136.50 || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $204.00 || $235.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,125.00 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00
|-
| 16S Amplicon || HTL || 48 || $2,375.00 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $812.50 || $910.00
|-
| Other Amplicon || HTL || 48 || $2,000.00 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || N/A || $945.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $196.25 || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $293.75 || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $340.60 || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $273.00 || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $375.70 || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,875.00 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,750.00 || $4,200.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,312.50 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,625.00 || $2,940.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,125.00 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,500.00 || $2,800.00
|-
| || || 96 || $4,000.00 || $4,480.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,750.00 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Standard Ligation || LSK || $200.00 || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $65.00 || $70.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $375.00 || $450.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $188.00 || $225.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $340.60 || $393.00
|-
| Direct RNA || RNA004 || $200.00 || $240.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| DNA || 96 || $875.00 || $980.00 ||
|-
| RNA || 96 || $1,000.00 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $375.00 || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,250.00 || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="5" | '''Assisted Service''' ||
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION'' ||
|-
| 5' RNA / 3' RNA - setup || setup || $1,268.75 || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,188.00 || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,437.50 || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,881.00 || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,375.00 || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $337.50 || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $4,875.00 || $5,460.00 ||
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $587.50 || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $56.00 || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq'' ||
|-
| ATACseq - setup || setup || $1,100.00 || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,094.00 || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)'' ||
|-
| MULTI - setup || setup || $2,250.00 || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,875.00 || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION'' ||
|-
| per session || <24h notice || $250.00 || $280.00 ||
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Test Array || slide || $1,217.00 || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $7,510.00 || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $5,688.00 || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $9,813.00 || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $75.00 || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $60.00 || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Slide Only || per slide || $188.00 || $210.00 ||
|-
| Optimization Only || per slide || $1,000.00 || $1,120.00 ||
|-
| Teton Panel || per slide || $9,000.00 || $10,080.00 ||
|-
| Atlas Short || per slide || $6,875.00 || $7,700.00 ||
|-
| Antibody Add-on || per slide || $450.00 || $504.00 ||
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $18.75 || $21.00 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $100.00 || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $37.50 || $42.00 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $200.00 || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $62.50 || $70.00 ||
|-
| qPCR only (Short read quantification test) || per sample || $31.25 || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $500.00 || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:''' ||
|-
| Fresh Frozen Curl || per curl || $60.00 || $66.00 ||
|-
| FFPE Curl || per curl || $75.00 || $84.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Dr.Oligo || Assisted || $2,500.00 +consumables || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,250.00 +consumables || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,000.00 || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,250.00 || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $625.00 || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,875.00 || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $144.00 || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $375.00 || $420.00 ||
|-
| || add Quench || $125.00 || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to external users.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! SIGN UP !! Notes
|-
| colspan="6" | '''COVARIS SONICATOR''' ||
|-
| COVARIS E220 || $25.00 || $28.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="6" | '''PIPPIN PREP''' ||
|-
| Gel Isolation || $93.75 || $105.00 || per gel (5 lanes) || TBA ||
|-
| Training || N/A || $50.00 || per session || TBA || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="6" | '''PLATE READER''' ||
|-
| Varioskan || $23.00 || $27.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="6" | '''ROBOTICS''' ||
|-
| Tecan EVO 150 || $93.75 || $105.00 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| non-filter tips || $10.00 || $11.20 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $15.00 || $16.80 || per box || ||
|-
| Training || $81.25 || $91.00 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]] ||
|-
| colspan="6" | '''NANODROP''' ||
|-
| Nanodrop || -- || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Informatics Support || per hour || $180.00 || $205.00 || Very limited support for external users
|-
| Informatics Share || per 0.1FTE || N/A || N/A || MIT only
|-
| Ingenuity Pathway Analysis || per dataset || N/A || $150.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || N/A || N/A ||
|-
| Tape Archive / Recovery || per tape || N/A || N/A ||
|-
| Informatics Training Classes || per session || N/A || N/A ||
|}

BioMicroCenter:Pricing

2026-04-16T19:49:02Z

Cstjohn: /* SHORT READ LIBRARY - RNA */

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,375.00 || $2,660.00
|-
| || One lane (~400M reads) || $1,250.00 || $1,400.00
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,312.50 || $3,710.00
|-
| || One lane (~400M reads) || $1,719.00 || $1,925.00
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,375.00 || $4,900.00
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 50 PE: F3 Lane (~100M reads) || $375.00 || $420.00
|-
| Rapid 300nt: F3 flowcell* || $2,250.00 || $2,520.00
|-
| 150 PE: F3 Lane (~100M reads) || $562.50 || $630.00
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit
|-
! Length !! Reads !! !!
|-
| 300nt || 5M || $893.75 || $1,001.00 || rowspan="9" | per flowcell
|-
| 600nt || 5M || $1,300.00 || $1,456.00
|-
| 100nt || 25M || $1,156.25 || $1,295.00
|-
| 300nt || 25M || $1,667.50 || $1,867.60
|-
| 600nt || 25M || $1,962.50 || $2,198.00
|-
| '''1000nt''' || 25M || $3,237.50 || $3,626.00
|-
| 100nt || 100M || $1,925.00 || $2,156.00
|-
| 300nt || 100M || $2,656.00 || $2,975.00
|-
| 600nt || 50M || $2,656.00 || $2,975.00
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,650.00 || $1,848.00 || rowspan="6" | per flowcell
|-
| 150nt V3 kits (eg. 75PE) || $1,787.50 || $2,002.00
|-
| 300nt V2 kit (eg. 150PE) || $1,993.75 || $2,233.00
|-
| 500nt V2 kit (eg. 250PE) || $2,200.00 || $2,464.00
|-
| 600nt V3 kit (eg. 300PE) || $2,750.00 || $3,080.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $962.50 || $1,078.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $43.75 || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $18.75 || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $31.25 || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $500.00 || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| 72 hour Flowcell || $1,500.00 || $1,680.00 || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| 72 hour Flowcell || colspan="2" | pricing in ilabs || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $37.50 || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $125.00 || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $136.50 || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $1,125.00 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $136.50 || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $204.00 || $235.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,125.00 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00
|-
| 16S Amplicon || HTL || 48 || $2,375.00 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $812.50 || $910.00
|-
| Other Amplicon || HTL || 48 || $2,000.00 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || N/A || $945.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $196.25 || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $293.75 || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $340.60 || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $273.00 || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $375.70 || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,875.00 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,750.00 || $4,200.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,312.50 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,625.00 || $2,940.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,125.00 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,500.00 || $2,800.00
|-
| || || 96 || $4,000.00 || $4,480.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,750.00 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Standard Ligation || LSK || $200.00 || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $65.00 || $70.00 ||
|-
| SMARTseq -> Ligation || Cv4+LSK || $375.00 || $450.00 ||
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $188.00 || $225.00 ||
|-
| Long Insert Prep || RAD (modified, target >100kb) || $340.60 || $393.00 ||
|-
| Direct RNA || RNA004 || $200.00 || $240.00 ||
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| DNA || 96 || $875.00 || $980.00 ||
|-
| RNA || 96 || $1,000.00 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $375.00 || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,250.00 || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="5" | '''Assisted Service''' ||
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION'' ||
|-
| 5' RNA / 3' RNA - setup || setup || $1,268.75 || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,188.00 || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,437.50 || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,881.00 || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,375.00 || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $337.50 || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $4,875.00 || $5,460.00 ||
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $587.50 || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $56.00 || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq'' ||
|-
| ATACseq - setup || setup || $1,100.00 || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,094.00 || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)'' ||
|-
| MULTI - setup || setup || $2,250.00 || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,875.00 || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION'' ||
|-
| per session || <24h notice || $250.00 || $280.00 ||
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Test Array || slide || $1,217.00 || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $7,510.00 || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $5,688.00 || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $9,813.00 || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $75.00 || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $60.00 || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Slide Only || per slide || $188.00 || $210.00 ||
|-
| Optimization Only || per slide || $1,000.00 || $1,120.00 ||
|-
| Teton Panel || per slide || $9,000.00 || $10,080.00 ||
|-
| Atlas Short || per slide || $6,875.00 || $7,700.00 ||
|-
| Antibody Add-on || per slide || $450.00 || $504.00 ||
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $18.75 || $21.00 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $100.00 || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $37.50 || $42.00 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $200.00 || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $62.50 || $70.00 ||
|-
| qPCR only (Short read quantification test) || per sample || $31.25 || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $500.00 || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:''' ||
|-
| Fresh Frozen Curl || per curl || $60.00 || $66.00 ||
|-
| FFPE Curl || per curl || $75.00 || $84.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Dr.Oligo || Assisted || $2,500.00 +consumables || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,250.00 +consumables || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,000.00 || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,250.00 || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $625.00 || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,875.00 || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $144.00 || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $375.00 || $420.00 ||
|-
| || add Quench || $125.00 || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to external users.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! SIGN UP !! Notes
|-
| colspan="6" | '''COVARIS SONICATOR''' ||
|-
| COVARIS E220 || $25.00 || $28.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="6" | '''PIPPIN PREP''' ||
|-
| Gel Isolation || $93.75 || $105.00 || per gel (5 lanes) || TBA ||
|-
| Training || N/A || $50.00 || per session || TBA || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="6" | '''PLATE READER''' ||
|-
| Varioskan || $23.00 || $27.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="6" | '''ROBOTICS''' ||
|-
| Tecan EVO 150 || $93.75 || $105.00 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| non-filter tips || $10.00 || $11.20 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $15.00 || $16.80 || per box || ||
|-
| Training || $81.25 || $91.00 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]] ||
|-
| colspan="6" | '''NANODROP''' ||
|-
| Nanodrop || -- || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Informatics Support || per hour || $180.00 || $205.00 || Very limited support for external users
|-
| Informatics Share || per 0.1FTE || N/A || N/A || MIT only
|-
| Ingenuity Pathway Analysis || per dataset || N/A || $150.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || N/A || N/A ||
|-
| Tape Archive / Recovery || per tape || N/A || N/A ||
|-
| Informatics Training Classes || per session || N/A || N/A ||
|}

BioMicroCenter:Pricing

2026-04-16T19:48:15Z

Cstjohn: /* SHORT READ LIBRARY - DNA */

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,375.00 || $2,660.00
|-
| || One lane (~400M reads) || $1,250.00 || $1,400.00
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,312.50 || $3,710.00
|-
| || One lane (~400M reads) || $1,719.00 || $1,925.00
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,375.00 || $4,900.00
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 50 PE: F3 Lane (~100M reads) || $375.00 || $420.00
|-
| Rapid 300nt: F3 flowcell* || $2,250.00 || $2,520.00
|-
| 150 PE: F3 Lane (~100M reads) || $562.50 || $630.00
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit
|-
! Length !! Reads !! !!
|-
| 300nt || 5M || $893.75 || $1,001.00 || rowspan="9" | per flowcell
|-
| 600nt || 5M || $1,300.00 || $1,456.00
|-
| 100nt || 25M || $1,156.25 || $1,295.00
|-
| 300nt || 25M || $1,667.50 || $1,867.60
|-
| 600nt || 25M || $1,962.50 || $2,198.00
|-
| '''1000nt''' || 25M || $3,237.50 || $3,626.00
|-
| 100nt || 100M || $1,925.00 || $2,156.00
|-
| 300nt || 100M || $2,656.00 || $2,975.00
|-
| 600nt || 50M || $2,656.00 || $2,975.00
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,650.00 || $1,848.00 || rowspan="6" | per flowcell
|-
| 150nt V3 kits (eg. 75PE) || $1,787.50 || $2,002.00
|-
| 300nt V2 kit (eg. 150PE) || $1,993.75 || $2,233.00
|-
| 500nt V2 kit (eg. 250PE) || $2,200.00 || $2,464.00
|-
| 600nt V3 kit (eg. 300PE) || $2,750.00 || $3,080.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $962.50 || $1,078.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $43.75 || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $18.75 || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $31.25 || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $500.00 || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| 72 hour Flowcell || $1,500.00 || $1,680.00 || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| 72 hour Flowcell || colspan="2" | pricing in ilabs || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $37.50 || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $125.00 || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $136.50 || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $1,125.00 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $136.50 || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $204.00 || $235.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,125.00 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00
|-
| 16S Amplicon || HTL || 48 || $2,375.00 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $812.50 || $910.00
|-
| Other Amplicon || HTL || 48 || $2,000.00 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || N/A || $945.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $196.25 || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $293.75 || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $340.60 || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $273.00 || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $375.70 || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. ||
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,875.00 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,750.00 || $4,200.00 ||
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,312.50 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,625.00 || $2,940.00 ||
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,125.00 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00 ||
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,500.00 || $2,800.00 ||
|-
| || || 96 || $4,000.00 || $4,480.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,750.00 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00 ||
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00 ||
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Standard Ligation || LSK || $200.00 || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $65.00 || $70.00 ||
|-
| SMARTseq -> Ligation || Cv4+LSK || $375.00 || $450.00 ||
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $188.00 || $225.00 ||
|-
| Long Insert Prep || RAD (modified, target >100kb) || $340.60 || $393.00 ||
|-
| Direct RNA || RNA004 || $200.00 || $240.00 ||
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| DNA || 96 || $875.00 || $980.00 ||
|-
| RNA || 96 || $1,000.00 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $375.00 || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,250.00 || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="5" | '''Assisted Service''' ||
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION'' ||
|-
| 5' RNA / 3' RNA - setup || setup || $1,268.75 || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,188.00 || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,437.50 || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,881.00 || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,375.00 || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $337.50 || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $4,875.00 || $5,460.00 ||
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $587.50 || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $56.00 || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq'' ||
|-
| ATACseq - setup || setup || $1,100.00 || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,094.00 || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)'' ||
|-
| MULTI - setup || setup || $2,250.00 || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,875.00 || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION'' ||
|-
| per session || <24h notice || $250.00 || $280.00 ||
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Test Array || slide || $1,217.00 || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $7,510.00 || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $5,688.00 || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $9,813.00 || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $75.00 || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $60.00 || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Slide Only || per slide || $188.00 || $210.00 ||
|-
| Optimization Only || per slide || $1,000.00 || $1,120.00 ||
|-
| Teton Panel || per slide || $9,000.00 || $10,080.00 ||
|-
| Atlas Short || per slide || $6,875.00 || $7,700.00 ||
|-
| Antibody Add-on || per slide || $450.00 || $504.00 ||
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $18.75 || $21.00 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $100.00 || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $37.50 || $42.00 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $200.00 || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $62.50 || $70.00 ||
|-
| qPCR only (Short read quantification test) || per sample || $31.25 || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $500.00 || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:''' ||
|-
| Fresh Frozen Curl || per curl || $60.00 || $66.00 ||
|-
| FFPE Curl || per curl || $75.00 || $84.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Dr.Oligo || Assisted || $2,500.00 +consumables || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,250.00 +consumables || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,000.00 || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,250.00 || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $625.00 || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,875.00 || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $144.00 || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $375.00 || $420.00 ||
|-
| || add Quench || $125.00 || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to external users.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! SIGN UP !! Notes
|-
| colspan="6" | '''COVARIS SONICATOR''' ||
|-
| COVARIS E220 || $25.00 || $28.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="6" | '''PIPPIN PREP''' ||
|-
| Gel Isolation || $93.75 || $105.00 || per gel (5 lanes) || TBA ||
|-
| Training || N/A || $50.00 || per session || TBA || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="6" | '''PLATE READER''' ||
|-
| Varioskan || $23.00 || $27.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="6" | '''ROBOTICS''' ||
|-
| Tecan EVO 150 || $93.75 || $105.00 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| non-filter tips || $10.00 || $11.20 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $15.00 || $16.80 || per box || ||
|-
| Training || $81.25 || $91.00 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]] ||
|-
| colspan="6" | '''NANODROP''' ||
|-
| Nanodrop || -- || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Informatics Support || per hour || $180.00 || $205.00 || Very limited support for external users
|-
| Informatics Share || per 0.1FTE || N/A || N/A || MIT only
|-
| Ingenuity Pathway Analysis || per dataset || N/A || $150.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || N/A || N/A ||
|-
| Tape Archive / Recovery || per tape || N/A || N/A ||
|-
| Informatics Training Classes || per session || N/A || N/A ||
|}

BioMicroCenter:Pricing

2026-04-16T19:47:33Z

Cstjohn: /* SHORT READ LIBRARY - DNA */

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,375.00 || $2,660.00
|-
| || One lane (~400M reads) || $1,250.00 || $1,400.00
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,312.50 || $3,710.00
|-
| || One lane (~400M reads) || $1,719.00 || $1,925.00
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,375.00 || $4,900.00
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 50 PE: F3 Lane (~100M reads) || $375.00 || $420.00
|-
| Rapid 300nt: F3 flowcell* || $2,250.00 || $2,520.00
|-
| 150 PE: F3 Lane (~100M reads) || $562.50 || $630.00
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit
|-
! Length !! Reads !! !!
|-
| 300nt || 5M || $893.75 || $1,001.00 || rowspan="9" | per flowcell
|-
| 600nt || 5M || $1,300.00 || $1,456.00
|-
| 100nt || 25M || $1,156.25 || $1,295.00
|-
| 300nt || 25M || $1,667.50 || $1,867.60
|-
| 600nt || 25M || $1,962.50 || $2,198.00
|-
| '''1000nt''' || 25M || $3,237.50 || $3,626.00
|-
| 100nt || 100M || $1,925.00 || $2,156.00
|-
| 300nt || 100M || $2,656.00 || $2,975.00
|-
| 600nt || 50M || $2,656.00 || $2,975.00
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,650.00 || $1,848.00 || rowspan="6" | per flowcell
|-
| 150nt V3 kits (eg. 75PE) || $1,787.50 || $2,002.00
|-
| 300nt V2 kit (eg. 150PE) || $1,993.75 || $2,233.00
|-
| 500nt V2 kit (eg. 250PE) || $2,200.00 || $2,464.00
|-
| 600nt V3 kit (eg. 300PE) || $2,750.00 || $3,080.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $962.50 || $1,078.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $43.75 || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $18.75 || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $31.25 || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $500.00 || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| 72 hour Flowcell || $1,500.00 || $1,680.00 || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| 72 hour Flowcell || colspan="2" | pricing in ilabs || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $37.50 || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $125.00 || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $136.50 || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $1,125.00 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $136.50 || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $204.00 || $235.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,125.00 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00
|-
| 16S Amplicon || HTL || 48 || $2,375.00 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $812.50 || $910.00
|-
| Other Amplicon || HTL || 48 || $2,000.00 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || N/A || $945.00
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $196.25 || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $293.75 || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $340.60 || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $273.00 || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $375.70 || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. ||
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,875.00 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,750.00 || $4,200.00 ||
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,312.50 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,625.00 || $2,940.00 ||
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,125.00 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00 ||
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,500.00 || $2,800.00 ||
|-
| || || 96 || $4,000.00 || $4,480.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,750.00 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00 ||
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00 ||
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Standard Ligation || LSK || $200.00 || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $65.00 || $70.00 ||
|-
| SMARTseq -> Ligation || Cv4+LSK || $375.00 || $450.00 ||
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $188.00 || $225.00 ||
|-
| Long Insert Prep || RAD (modified, target >100kb) || $340.60 || $393.00 ||
|-
| Direct RNA || RNA004 || $200.00 || $240.00 ||
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| DNA || 96 || $875.00 || $980.00 ||
|-
| RNA || 96 || $1,000.00 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $375.00 || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,250.00 || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="5" | '''Assisted Service''' ||
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION'' ||
|-
| 5' RNA / 3' RNA - setup || setup || $1,268.75 || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,188.00 || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,437.50 || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,881.00 || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,375.00 || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $337.50 || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $4,875.00 || $5,460.00 ||
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $587.50 || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $56.00 || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq'' ||
|-
| ATACseq - setup || setup || $1,100.00 || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,094.00 || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)'' ||
|-
| MULTI - setup || setup || $2,250.00 || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,875.00 || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION'' ||
|-
| per session || <24h notice || $250.00 || $280.00 ||
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Test Array || slide || $1,217.00 || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $7,510.00 || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $5,688.00 || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $9,813.00 || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $75.00 || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $60.00 || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Slide Only || per slide || $188.00 || $210.00 ||
|-
| Optimization Only || per slide || $1,000.00 || $1,120.00 ||
|-
| Teton Panel || per slide || $9,000.00 || $10,080.00 ||
|-
| Atlas Short || per slide || $6,875.00 || $7,700.00 ||
|-
| Antibody Add-on || per slide || $450.00 || $504.00 ||
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $18.75 || $21.00 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $100.00 || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $37.50 || $42.00 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $200.00 || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $62.50 || $70.00 ||
|-
| qPCR only (Short read quantification test) || per sample || $31.25 || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $500.00 || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:''' ||
|-
| Fresh Frozen Curl || per curl || $60.00 || $66.00 ||
|-
| FFPE Curl || per curl || $75.00 || $84.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Dr.Oligo || Assisted || $2,500.00 +consumables || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,250.00 +consumables || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,000.00 || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,250.00 || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $625.00 || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,875.00 || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $144.00 || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $375.00 || $420.00 ||
|-
| || add Quench || $125.00 || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to external users.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! SIGN UP !! Notes
|-
| colspan="6" | '''COVARIS SONICATOR''' ||
|-
| COVARIS E220 || $25.00 || $28.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="6" | '''PIPPIN PREP''' ||
|-
| Gel Isolation || $93.75 || $105.00 || per gel (5 lanes) || TBA ||
|-
| Training || N/A || $50.00 || per session || TBA || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="6" | '''PLATE READER''' ||
|-
| Varioskan || $23.00 || $27.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="6" | '''ROBOTICS''' ||
|-
| Tecan EVO 150 || $93.75 || $105.00 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| non-filter tips || $10.00 || $11.20 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $15.00 || $16.80 || per box || ||
|-
| Training || $81.25 || $91.00 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]] ||
|-
| colspan="6" | '''NANODROP''' ||
|-
| Nanodrop || -- || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Informatics Support || per hour || $180.00 || $205.00 || Very limited support for external users
|-
| Informatics Share || per 0.1FTE || N/A || N/A || MIT only
|-
| Ingenuity Pathway Analysis || per dataset || N/A || $150.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || N/A || N/A ||
|-
| Tape Archive / Recovery || per tape || N/A || N/A ||
|-
| Informatics Training Classes || per session || N/A || N/A ||
|}

BioMicroCenter:Pricing

2026-04-16T19:46:36Z

Cstjohn: /* LONG READ SEQUENCING */

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,375.00 || $2,660.00
|-
| || One lane (~400M reads) || $1,250.00 || $1,400.00
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,312.50 || $3,710.00
|-
| || One lane (~400M reads) || $1,719.00 || $1,925.00
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,375.00 || $4,900.00
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 50 PE: F3 Lane (~100M reads) || $375.00 || $420.00
|-
| Rapid 300nt: F3 flowcell* || $2,250.00 || $2,520.00
|-
| 150 PE: F3 Lane (~100M reads) || $562.50 || $630.00
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit
|-
! Length !! Reads !! !!
|-
| 300nt || 5M || $893.75 || $1,001.00 || rowspan="9" | per flowcell
|-
| 600nt || 5M || $1,300.00 || $1,456.00
|-
| 100nt || 25M || $1,156.25 || $1,295.00
|-
| 300nt || 25M || $1,667.50 || $1,867.60
|-
| 600nt || 25M || $1,962.50 || $2,198.00
|-
| '''1000nt''' || 25M || $3,237.50 || $3,626.00
|-
| 100nt || 100M || $1,925.00 || $2,156.00
|-
| 300nt || 100M || $2,656.00 || $2,975.00
|-
| 600nt || 50M || $2,656.00 || $2,975.00
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,650.00 || $1,848.00 || rowspan="6" | per flowcell
|-
| 150nt V3 kits (eg. 75PE) || $1,787.50 || $2,002.00
|-
| 300nt V2 kit (eg. 150PE) || $1,993.75 || $2,233.00
|-
| 500nt V2 kit (eg. 250PE) || $2,200.00 || $2,464.00
|-
| 600nt V3 kit (eg. 300PE) || $2,750.00 || $3,080.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $962.50 || $1,078.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $43.75 || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $18.75 || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $31.25 || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $500.00 || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| 72 hour Flowcell || $1,500.00 || $1,680.00 || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| 72 hour Flowcell || colspan="2" | pricing in ilabs || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $37.50 || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $125.00 || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| LIGATION BASED NEB UltraII || STD || per sample || $136.50 || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. ||
|-
| HTL NEB UltraII || HTL || 24 || $1,125.00 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00 ||
|-
| Tagmentation Nextera-XT || STD || per Sample || $136.50 || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $204.00 || $235.00 ||
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,125.00 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00 ||
|-
| 16S Amplicon || HTL || 48 || $2,375.00 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $812.50 || $910.00 ||
|-
| Other Amplicon || HTL || 48 || $2,000.00 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || N/A || $945.00 ||
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00 ||
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $196.25 || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $293.75 || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $340.60 || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $273.00 || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $375.70 || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. ||
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,875.00 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,750.00 || $4,200.00 ||
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,312.50 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,625.00 || $2,940.00 ||
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,125.00 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00 ||
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,500.00 || $2,800.00 ||
|-
| || || 96 || $4,000.00 || $4,480.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,750.00 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00 ||
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00 ||
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Standard Ligation || LSK || $200.00 || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $65.00 || $70.00 ||
|-
| SMARTseq -> Ligation || Cv4+LSK || $375.00 || $450.00 ||
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $188.00 || $225.00 ||
|-
| Long Insert Prep || RAD (modified, target >100kb) || $340.60 || $393.00 ||
|-
| Direct RNA || RNA004 || $200.00 || $240.00 ||
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| DNA || 96 || $875.00 || $980.00 ||
|-
| RNA || 96 || $1,000.00 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $375.00 || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,250.00 || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="5" | '''Assisted Service''' ||
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION'' ||
|-
| 5' RNA / 3' RNA - setup || setup || $1,268.75 || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,188.00 || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,437.50 || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,881.00 || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,375.00 || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $337.50 || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $4,875.00 || $5,460.00 ||
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $587.50 || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $56.00 || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq'' ||
|-
| ATACseq - setup || setup || $1,100.00 || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,094.00 || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)'' ||
|-
| MULTI - setup || setup || $2,250.00 || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,875.00 || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION'' ||
|-
| per session || <24h notice || $250.00 || $280.00 ||
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Test Array || slide || $1,217.00 || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $7,510.00 || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $5,688.00 || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $9,813.00 || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $75.00 || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $60.00 || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Slide Only || per slide || $188.00 || $210.00 ||
|-
| Optimization Only || per slide || $1,000.00 || $1,120.00 ||
|-
| Teton Panel || per slide || $9,000.00 || $10,080.00 ||
|-
| Atlas Short || per slide || $6,875.00 || $7,700.00 ||
|-
| Antibody Add-on || per slide || $450.00 || $504.00 ||
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $18.75 || $21.00 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $100.00 || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $37.50 || $42.00 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $200.00 || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $62.50 || $70.00 ||
|-
| qPCR only (Short read quantification test) || per sample || $31.25 || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $500.00 || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:''' ||
|-
| Fresh Frozen Curl || per curl || $60.00 || $66.00 ||
|-
| FFPE Curl || per curl || $75.00 || $84.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Dr.Oligo || Assisted || $2,500.00 +consumables || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,250.00 +consumables || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,000.00 || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,250.00 || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $625.00 || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,875.00 || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $144.00 || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $375.00 || $420.00 ||
|-
| || add Quench || $125.00 || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to external users.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! SIGN UP !! Notes
|-
| colspan="6" | '''COVARIS SONICATOR''' ||
|-
| COVARIS E220 || $25.00 || $28.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="6" | '''PIPPIN PREP''' ||
|-
| Gel Isolation || $93.75 || $105.00 || per gel (5 lanes) || TBA ||
|-
| Training || N/A || $50.00 || per session || TBA || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="6" | '''PLATE READER''' ||
|-
| Varioskan || $23.00 || $27.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="6" | '''ROBOTICS''' ||
|-
| Tecan EVO 150 || $93.75 || $105.00 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| non-filter tips || $10.00 || $11.20 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $15.00 || $16.80 || per box || ||
|-
| Training || $81.25 || $91.00 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]] ||
|-
| colspan="6" | '''NANODROP''' ||
|-
| Nanodrop || -- || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Informatics Support || per hour || $180.00 || $205.00 || Very limited support for external users
|-
| Informatics Share || per 0.1FTE || N/A || N/A || MIT only
|-
| Ingenuity Pathway Analysis || per dataset || N/A || $150.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || N/A || N/A ||
|-
| Tape Archive / Recovery || per tape || N/A || N/A ||
|-
| Informatics Training Classes || per session || N/A || N/A ||
|}

BioMicroCenter:Pricing

2026-04-16T19:46:20Z

Cstjohn: /* LONG READ SEQUENCING */

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,375.00 || $2,660.00
|-
| || One lane (~400M reads) || $1,250.00 || $1,400.00
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,312.50 || $3,710.00
|-
| || One lane (~400M reads) || $1,719.00 || $1,925.00
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,375.00 || $4,900.00
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 50 PE: F3 Lane (~100M reads) || $375.00 || $420.00
|-
| Rapid 300nt: F3 flowcell* || $2,250.00 || $2,520.00
|-
| 150 PE: F3 Lane (~100M reads) || $562.50 || $630.00
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit
|-
! Length !! Reads !! !!
|-
| 300nt || 5M || $893.75 || $1,001.00 || rowspan="9" | per flowcell
|-
| 600nt || 5M || $1,300.00 || $1,456.00
|-
| 100nt || 25M || $1,156.25 || $1,295.00
|-
| 300nt || 25M || $1,667.50 || $1,867.60
|-
| 600nt || 25M || $1,962.50 || $2,198.00
|-
| '''1000nt''' || 25M || $3,237.50 || $3,626.00
|-
| 100nt || 100M || $1,925.00 || $2,156.00
|-
| 300nt || 100M || $2,656.00 || $2,975.00
|-
| 600nt || 50M || $2,656.00 || $2,975.00
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,650.00 || $1,848.00 || rowspan="6" | per flowcell
|-
| 150nt V3 kits (eg. 75PE) || $1,787.50 || $2,002.00
|-
| 300nt V2 kit (eg. 150PE) || $1,993.75 || $2,233.00
|-
| 500nt V2 kit (eg. 250PE) || $2,200.00 || $2,464.00
|-
| 600nt V3 kit (eg. 300PE) || $2,750.00 || $3,080.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $962.50 || $1,078.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $43.75 || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $18.75 || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $31.25 || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $500.00 || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| 72 hour Flowcell || $1,500.00 || $1,680.00 || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| 72 hour Flowcell || colspan="2" | pricing in ilabs || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $37.50 || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $125.00 || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| LIGATION BASED NEB UltraII || STD || per sample || $136.50 || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. ||
|-
| HTL NEB UltraII || HTL || 24 || $1,125.00 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00 ||
|-
| Tagmentation Nextera-XT || STD || per Sample || $136.50 || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $204.00 || $235.00 ||
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,125.00 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00 ||
|-
| 16S Amplicon || HTL || 48 || $2,375.00 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $812.50 || $910.00 ||
|-
| Other Amplicon || HTL || 48 || $2,000.00 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || N/A || $945.00 ||
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00 ||
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $196.25 || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $293.75 || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $340.60 || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $273.00 || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $375.70 || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. ||
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,875.00 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,750.00 || $4,200.00 ||
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,312.50 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,625.00 || $2,940.00 ||
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,125.00 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00 ||
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,500.00 || $2,800.00 ||
|-
| || || 96 || $4,000.00 || $4,480.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,750.00 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00 ||
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00 ||
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Standard Ligation || LSK || $200.00 || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $65.00 || $70.00 ||
|-
| SMARTseq -> Ligation || Cv4+LSK || $375.00 || $450.00 ||
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $188.00 || $225.00 ||
|-
| Long Insert Prep || RAD (modified, target >100kb) || $340.60 || $393.00 ||
|-
| Direct RNA || RNA004 || $200.00 || $240.00 ||
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| DNA || 96 || $875.00 || $980.00 ||
|-
| RNA || 96 || $1,000.00 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $375.00 || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,250.00 || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="5" | '''Assisted Service''' ||
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION'' ||
|-
| 5' RNA / 3' RNA - setup || setup || $1,268.75 || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,188.00 || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,437.50 || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,881.00 || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,375.00 || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $337.50 || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $4,875.00 || $5,460.00 ||
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $587.50 || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $56.00 || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq'' ||
|-
| ATACseq - setup || setup || $1,100.00 || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,094.00 || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)'' ||
|-
| MULTI - setup || setup || $2,250.00 || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,875.00 || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION'' ||
|-
| per session || <24h notice || $250.00 || $280.00 ||
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Test Array || slide || $1,217.00 || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $7,510.00 || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $5,688.00 || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $9,813.00 || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $75.00 || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $60.00 || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Slide Only || per slide || $188.00 || $210.00 ||
|-
| Optimization Only || per slide || $1,000.00 || $1,120.00 ||
|-
| Teton Panel || per slide || $9,000.00 || $10,080.00 ||
|-
| Atlas Short || per slide || $6,875.00 || $7,700.00 ||
|-
| Antibody Add-on || per slide || $450.00 || $504.00 ||
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $18.75 || $21.00 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $100.00 || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $37.50 || $42.00 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $200.00 || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $62.50 || $70.00 ||
|-
| qPCR only (Short read quantification test) || per sample || $31.25 || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $500.00 || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:''' ||
|-
| Fresh Frozen Curl || per curl || $60.00 || $66.00 ||
|-
| FFPE Curl || per curl || $75.00 || $84.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Dr.Oligo || Assisted || $2,500.00 +consumables || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,250.00 +consumables || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,000.00 || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,250.00 || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $625.00 || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,875.00 || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $144.00 || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $375.00 || $420.00 ||
|-
| || add Quench || $125.00 || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to external users.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! SIGN UP !! Notes
|-
| colspan="6" | '''COVARIS SONICATOR''' ||
|-
| COVARIS E220 || $25.00 || $28.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="6" | '''PIPPIN PREP''' ||
|-
| Gel Isolation || $93.75 || $105.00 || per gel (5 lanes) || TBA ||
|-
| Training || N/A || $50.00 || per session || TBA || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="6" | '''PLATE READER''' ||
|-
| Varioskan || $23.00 || $27.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="6" | '''ROBOTICS''' ||
|-
| Tecan EVO 150 || $93.75 || $105.00 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| non-filter tips || $10.00 || $11.20 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $15.00 || $16.80 || per box || ||
|-
| Training || $81.25 || $91.00 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]] ||
|-
| colspan="6" | '''NANODROP''' ||
|-
| Nanodrop || -- || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Informatics Support || per hour || $180.00 || $205.00 || Very limited support for external users
|-
| Informatics Share || per 0.1FTE || N/A || N/A || MIT only
|-
| Ingenuity Pathway Analysis || per dataset || N/A || $150.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || N/A || N/A ||
|-
| Tape Archive / Recovery || per tape || N/A || N/A ||
|-
| Informatics Training Classes || per session || N/A || N/A ||
|}

BioMicroCenter:Pricing

2026-04-16T19:45:25Z

Cstjohn: /* MiSeq classic */

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,375.00 || $2,660.00
|-
| || One lane (~400M reads) || $1,250.00 || $1,400.00
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,312.50 || $3,710.00
|-
| || One lane (~400M reads) || $1,719.00 || $1,925.00
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,375.00 || $4,900.00
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 50 PE: F3 Lane (~100M reads) || $375.00 || $420.00
|-
| Rapid 300nt: F3 flowcell* || $2,250.00 || $2,520.00
|-
| 150 PE: F3 Lane (~100M reads) || $562.50 || $630.00
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit
|-
! Length !! Reads !! !!
|-
| 300nt || 5M || $893.75 || $1,001.00 || rowspan="9" | per flowcell
|-
| 600nt || 5M || $1,300.00 || $1,456.00
|-
| 100nt || 25M || $1,156.25 || $1,295.00
|-
| 300nt || 25M || $1,667.50 || $1,867.60
|-
| 600nt || 25M || $1,962.50 || $2,198.00
|-
| '''1000nt''' || 25M || $3,237.50 || $3,626.00
|-
| 100nt || 100M || $1,925.00 || $2,156.00
|-
| 300nt || 100M || $2,656.00 || $2,975.00
|-
| 600nt || 50M || $2,656.00 || $2,975.00
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,650.00 || $1,848.00 || rowspan="6" | per flowcell
|-
| 150nt V3 kits (eg. 75PE) || $1,787.50 || $2,002.00
|-
| 300nt V2 kit (eg. 150PE) || $1,993.75 || $2,233.00
|-
| 500nt V2 kit (eg. 250PE) || $2,200.00 || $2,464.00
|-
| 600nt V3 kit (eg. 300PE) || $2,750.00 || $3,080.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $962.50 || $1,078.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $43.75 || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $18.75 || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $31.25 || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $500.00 || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| 72 hour Flowcell || $1,500.00 || $1,680.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| 72 hour Flowcell || colspan="2" | pricing in ilabs || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $37.50 || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $125.00 || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| LIGATION BASED NEB UltraII || STD || per sample || $136.50 || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. ||
|-
| HTL NEB UltraII || HTL || 24 || $1,125.00 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00 ||
|-
| Tagmentation Nextera-XT || STD || per Sample || $136.50 || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $204.00 || $235.00 ||
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,125.00 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00 ||
|-
| 16S Amplicon || HTL || 48 || $2,375.00 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $812.50 || $910.00 ||
|-
| Other Amplicon || HTL || 48 || $2,000.00 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || N/A || $945.00 ||
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00 ||
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $196.25 || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $293.75 || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $340.60 || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $273.00 || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $375.70 || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. ||
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,875.00 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,750.00 || $4,200.00 ||
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,312.50 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,625.00 || $2,940.00 ||
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,125.00 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00 ||
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,500.00 || $2,800.00 ||
|-
| || || 96 || $4,000.00 || $4,480.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,750.00 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00 ||
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00 ||
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Standard Ligation || LSK || $200.00 || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $65.00 || $70.00 ||
|-
| SMARTseq -> Ligation || Cv4+LSK || $375.00 || $450.00 ||
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $188.00 || $225.00 ||
|-
| Long Insert Prep || RAD (modified, target >100kb) || $340.60 || $393.00 ||
|-
| Direct RNA || RNA004 || $200.00 || $240.00 ||
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| DNA || 96 || $875.00 || $980.00 ||
|-
| RNA || 96 || $1,000.00 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $375.00 || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,250.00 || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="5" | '''Assisted Service''' ||
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION'' ||
|-
| 5' RNA / 3' RNA - setup || setup || $1,268.75 || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,188.00 || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,437.50 || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,881.00 || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,375.00 || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $337.50 || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $4,875.00 || $5,460.00 ||
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $587.50 || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $56.00 || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq'' ||
|-
| ATACseq - setup || setup || $1,100.00 || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,094.00 || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)'' ||
|-
| MULTI - setup || setup || $2,250.00 || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,875.00 || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION'' ||
|-
| per session || <24h notice || $250.00 || $280.00 ||
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Test Array || slide || $1,217.00 || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $7,510.00 || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $5,688.00 || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $9,813.00 || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $75.00 || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $60.00 || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Slide Only || per slide || $188.00 || $210.00 ||
|-
| Optimization Only || per slide || $1,000.00 || $1,120.00 ||
|-
| Teton Panel || per slide || $9,000.00 || $10,080.00 ||
|-
| Atlas Short || per slide || $6,875.00 || $7,700.00 ||
|-
| Antibody Add-on || per slide || $450.00 || $504.00 ||
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $18.75 || $21.00 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $100.00 || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $37.50 || $42.00 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $200.00 || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $62.50 || $70.00 ||
|-
| qPCR only (Short read quantification test) || per sample || $31.25 || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $500.00 || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:''' ||
|-
| Fresh Frozen Curl || per curl || $60.00 || $66.00 ||
|-
| FFPE Curl || per curl || $75.00 || $84.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Dr.Oligo || Assisted || $2,500.00 +consumables || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,250.00 +consumables || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,000.00 || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,250.00 || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $625.00 || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,875.00 || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $144.00 || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $375.00 || $420.00 ||
|-
| || add Quench || $125.00 || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to external users.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! SIGN UP !! Notes
|-
| colspan="6" | '''COVARIS SONICATOR''' ||
|-
| COVARIS E220 || $25.00 || $28.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="6" | '''PIPPIN PREP''' ||
|-
| Gel Isolation || $93.75 || $105.00 || per gel (5 lanes) || TBA ||
|-
| Training || N/A || $50.00 || per session || TBA || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="6" | '''PLATE READER''' ||
|-
| Varioskan || $23.00 || $27.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="6" | '''ROBOTICS''' ||
|-
| Tecan EVO 150 || $93.75 || $105.00 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| non-filter tips || $10.00 || $11.20 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $15.00 || $16.80 || per box || ||
|-
| Training || $81.25 || $91.00 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]] ||
|-
| colspan="6" | '''NANODROP''' ||
|-
| Nanodrop || -- || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Informatics Support || per hour || $180.00 || $205.00 || Very limited support for external users
|-
| Informatics Share || per 0.1FTE || N/A || N/A || MIT only
|-
| Ingenuity Pathway Analysis || per dataset || N/A || $150.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || N/A || N/A ||
|-
| Tape Archive / Recovery || per tape || N/A || N/A ||
|-
| Informatics Training Classes || per session || N/A || N/A ||
|}

BioMicroCenter:Pricing

2026-04-16T19:43:59Z

Cstjohn: /* MiSeq classic */

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,375.00 || $2,660.00
|-
| || One lane (~400M reads) || $1,250.00 || $1,400.00
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,312.50 || $3,710.00
|-
| || One lane (~400M reads) || $1,719.00 || $1,925.00
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,375.00 || $4,900.00
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 50 PE: F3 Lane (~100M reads) || $375.00 || $420.00
|-
| Rapid 300nt: F3 flowcell* || $2,250.00 || $2,520.00
|-
| 150 PE: F3 Lane (~100M reads) || $562.50 || $630.00
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit
|-
! Length !! Reads !! !!
|-
| 300nt || 5M || $893.75 || $1,001.00 || rowspan="9" | per flowcell
|-
| 600nt || 5M || $1,300.00 || $1,456.00
|-
| 100nt || 25M || $1,156.25 || $1,295.00
|-
| 300nt || 25M || $1,667.50 || $1,867.60
|-
| 600nt || 25M || $1,962.50 || $2,198.00
|-
| '''1000nt''' || 25M || $3,237.50 || $3,626.00
|-
| 100nt || 100M || $1,925.00 || $2,156.00
|-
| 300nt || 100M || $2,656.00 || $2,975.00
|-
| 600nt || 50M || $2,656.00 || $2,975.00
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,650.00 || $1,848.00 || rowspan="6" | per flowcell
| 150nt V3 kits (eg. 75PE) || $1,787.50 || $2,002.00
|-
| 300nt V2 kit (eg. 150PE) || $1,993.75 || $2,233.00
|-
| 500nt V2 kit (eg. 250PE) || $2,200.00 || $2,464.00
|-
| 600nt V3 kit (eg. 300PE) || $2,750.00 || $3,080.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $962.50 || $1,078.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $43.75 || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $18.75 || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $31.25 || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $500.00 || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| 72 hour Flowcell || $1,500.00 || $1,680.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| 72 hour Flowcell || colspan="2" | pricing in ilabs || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $37.50 || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $125.00 || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| LIGATION BASED NEB UltraII || STD || per sample || $136.50 || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. ||
|-
| HTL NEB UltraII || HTL || 24 || $1,125.00 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00 ||
|-
| Tagmentation Nextera-XT || STD || per Sample || $136.50 || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $204.00 || $235.00 ||
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,125.00 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00 ||
|-
| 16S Amplicon || HTL || 48 || $2,375.00 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $812.50 || $910.00 ||
|-
| Other Amplicon || HTL || 48 || $2,000.00 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || N/A || $945.00 ||
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00 ||
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $196.25 || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $293.75 || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $340.60 || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $273.00 || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $375.70 || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. ||
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,875.00 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,750.00 || $4,200.00 ||
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,312.50 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,625.00 || $2,940.00 ||
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,125.00 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00 ||
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,500.00 || $2,800.00 ||
|-
| || || 96 || $4,000.00 || $4,480.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,750.00 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00 ||
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00 ||
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Standard Ligation || LSK || $200.00 || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $65.00 || $70.00 ||
|-
| SMARTseq -> Ligation || Cv4+LSK || $375.00 || $450.00 ||
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $188.00 || $225.00 ||
|-
| Long Insert Prep || RAD (modified, target >100kb) || $340.60 || $393.00 ||
|-
| Direct RNA || RNA004 || $200.00 || $240.00 ||
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| DNA || 96 || $875.00 || $980.00 ||
|-
| RNA || 96 || $1,000.00 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $375.00 || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,250.00 || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="5" | '''Assisted Service''' ||
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION'' ||
|-
| 5' RNA / 3' RNA - setup || setup || $1,268.75 || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,188.00 || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,437.50 || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,881.00 || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,375.00 || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $337.50 || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $4,875.00 || $5,460.00 ||
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $587.50 || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $56.00 || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq'' ||
|-
| ATACseq - setup || setup || $1,100.00 || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,094.00 || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)'' ||
|-
| MULTI - setup || setup || $2,250.00 || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,875.00 || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION'' ||
|-
| per session || <24h notice || $250.00 || $280.00 ||
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Test Array || slide || $1,217.00 || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $7,510.00 || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $5,688.00 || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $9,813.00 || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $75.00 || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $60.00 || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Slide Only || per slide || $188.00 || $210.00 ||
|-
| Optimization Only || per slide || $1,000.00 || $1,120.00 ||
|-
| Teton Panel || per slide || $9,000.00 || $10,080.00 ||
|-
| Atlas Short || per slide || $6,875.00 || $7,700.00 ||
|-
| Antibody Add-on || per slide || $450.00 || $504.00 ||
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $18.75 || $21.00 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $100.00 || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $37.50 || $42.00 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $200.00 || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $62.50 || $70.00 ||
|-
| qPCR only (Short read quantification test) || per sample || $31.25 || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $500.00 || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:''' ||
|-
| Fresh Frozen Curl || per curl || $60.00 || $66.00 ||
|-
| FFPE Curl || per curl || $75.00 || $84.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Dr.Oligo || Assisted || $2,500.00 +consumables || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,250.00 +consumables || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,000.00 || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,250.00 || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $625.00 || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,875.00 || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $144.00 || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $375.00 || $420.00 ||
|-
| || add Quench || $125.00 || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to external users.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! SIGN UP !! Notes
|-
| colspan="6" | '''COVARIS SONICATOR''' ||
|-
| COVARIS E220 || $25.00 || $28.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="6" | '''PIPPIN PREP''' ||
|-
| Gel Isolation || $93.75 || $105.00 || per gel (5 lanes) || TBA ||
|-
| Training || N/A || $50.00 || per session || TBA || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="6" | '''PLATE READER''' ||
|-
| Varioskan || $23.00 || $27.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="6" | '''ROBOTICS''' ||
|-
| Tecan EVO 150 || $93.75 || $105.00 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| non-filter tips || $10.00 || $11.20 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $15.00 || $16.80 || per box || ||
|-
| Training || $81.25 || $91.00 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]] ||
|-
| colspan="6" | '''NANODROP''' ||
|-
| Nanodrop || -- || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Informatics Support || per hour || $180.00 || $205.00 || Very limited support for external users
|-
| Informatics Share || per 0.1FTE || N/A || N/A || MIT only
|-
| Ingenuity Pathway Analysis || per dataset || N/A || $150.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || N/A || N/A ||
|-
| Tape Archive / Recovery || per tape || N/A || N/A ||
|-
| Informatics Training Classes || per session || N/A || N/A ||
|}

BioMicroCenter:Pricing

2026-04-16T19:43:32Z

Cstjohn: /* MiSeq i100 Walkup */

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,375.00 || $2,660.00
|-
| || One lane (~400M reads) || $1,250.00 || $1,400.00
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,312.50 || $3,710.00
|-
| || One lane (~400M reads) || $1,719.00 || $1,925.00
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,375.00 || $4,900.00
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 50 PE: F3 Lane (~100M reads) || $375.00 || $420.00
|-
| Rapid 300nt: F3 flowcell* || $2,250.00 || $2,520.00
|-
| 150 PE: F3 Lane (~100M reads) || $562.50 || $630.00
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit
|-
! Length !! Reads !! !!
|-
| 300nt || 5M || $893.75 || $1,001.00 || rowspan="9" | per flowcell
|-
| 600nt || 5M || $1,300.00 || $1,456.00
|-
| 100nt || 25M || $1,156.25 || $1,295.00
|-
| 300nt || 25M || $1,667.50 || $1,867.60
|-
| 600nt || 25M || $1,962.50 || $2,198.00
|-
| '''1000nt''' || 25M || $3,237.50 || $3,626.00
|-
| 100nt || 100M || $1,925.00 || $2,156.00
|-
| 300nt || 100M || $2,656.00 || $2,975.00
|-
| 600nt || 50M || $2,656.00 || $2,975.00
|}

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,650.00 || $1,848.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $1,787.50 || $2,002.00 ||
|-
| 300nt V2 kit (eg. 150PE) || $1,993.75 || $2,233.00 ||
|-
| 500nt V2 kit (eg. 250PE) || $2,200.00 || $2,464.00 ||
|-
| 600nt V3 kit (eg. 300PE) || $2,750.00 || $3,080.00 ||
|-
| 500nt '''NANO''' Run (eg. 250PE) || $962.50 || $1,078.00 ||
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $43.75 || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $18.75 || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $31.25 || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $500.00 || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| 72 hour Flowcell || $1,500.00 || $1,680.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| 72 hour Flowcell || colspan="2" | pricing in ilabs || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $37.50 || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $125.00 || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| LIGATION BASED NEB UltraII || STD || per sample || $136.50 || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. ||
|-
| HTL NEB UltraII || HTL || 24 || $1,125.00 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00 ||
|-
| Tagmentation Nextera-XT || STD || per Sample || $136.50 || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $204.00 || $235.00 ||
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,125.00 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00 ||
|-
| 16S Amplicon || HTL || 48 || $2,375.00 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $812.50 || $910.00 ||
|-
| Other Amplicon || HTL || 48 || $2,000.00 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || N/A || $945.00 ||
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00 ||
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $196.25 || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $293.75 || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $340.60 || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $273.00 || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $375.70 || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. ||
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,875.00 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,750.00 || $4,200.00 ||
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,312.50 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,625.00 || $2,940.00 ||
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,125.00 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00 ||
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,500.00 || $2,800.00 ||
|-
| || || 96 || $4,000.00 || $4,480.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,750.00 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00 ||
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00 ||
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Standard Ligation || LSK || $200.00 || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $65.00 || $70.00 ||
|-
| SMARTseq -> Ligation || Cv4+LSK || $375.00 || $450.00 ||
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $188.00 || $225.00 ||
|-
| Long Insert Prep || RAD (modified, target >100kb) || $340.60 || $393.00 ||
|-
| Direct RNA || RNA004 || $200.00 || $240.00 ||
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| DNA || 96 || $875.00 || $980.00 ||
|-
| RNA || 96 || $1,000.00 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $375.00 || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,250.00 || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="5" | '''Assisted Service''' ||
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION'' ||
|-
| 5' RNA / 3' RNA - setup || setup || $1,268.75 || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,188.00 || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,437.50 || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,881.00 || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,375.00 || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $337.50 || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $4,875.00 || $5,460.00 ||
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $587.50 || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $56.00 || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq'' ||
|-
| ATACseq - setup || setup || $1,100.00 || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,094.00 || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)'' ||
|-
| MULTI - setup || setup || $2,250.00 || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,875.00 || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION'' ||
|-
| per session || <24h notice || $250.00 || $280.00 ||
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Test Array || slide || $1,217.00 || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $7,510.00 || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $5,688.00 || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $9,813.00 || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $75.00 || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $60.00 || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Slide Only || per slide || $188.00 || $210.00 ||
|-
| Optimization Only || per slide || $1,000.00 || $1,120.00 ||
|-
| Teton Panel || per slide || $9,000.00 || $10,080.00 ||
|-
| Atlas Short || per slide || $6,875.00 || $7,700.00 ||
|-
| Antibody Add-on || per slide || $450.00 || $504.00 ||
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $18.75 || $21.00 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $100.00 || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $37.50 || $42.00 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $200.00 || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $62.50 || $70.00 ||
|-
| qPCR only (Short read quantification test) || per sample || $31.25 || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $500.00 || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:''' ||
|-
| Fresh Frozen Curl || per curl || $60.00 || $66.00 ||
|-
| FFPE Curl || per curl || $75.00 || $84.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Dr.Oligo || Assisted || $2,500.00 +consumables || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,250.00 +consumables || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,000.00 || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,250.00 || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $625.00 || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,875.00 || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $144.00 || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $375.00 || $420.00 ||
|-
| || add Quench || $125.00 || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to external users.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! SIGN UP !! Notes
|-
| colspan="6" | '''COVARIS SONICATOR''' ||
|-
| COVARIS E220 || $25.00 || $28.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="6" | '''PIPPIN PREP''' ||
|-
| Gel Isolation || $93.75 || $105.00 || per gel (5 lanes) || TBA ||
|-
| Training || N/A || $50.00 || per session || TBA || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="6" | '''PLATE READER''' ||
|-
| Varioskan || $23.00 || $27.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="6" | '''ROBOTICS''' ||
|-
| Tecan EVO 150 || $93.75 || $105.00 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| non-filter tips || $10.00 || $11.20 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $15.00 || $16.80 || per box || ||
|-
| Training || $81.25 || $91.00 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]] ||
|-
| colspan="6" | '''NANODROP''' ||
|-
| Nanodrop || -- || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Informatics Support || per hour || $180.00 || $205.00 || Very limited support for external users
|-
| Informatics Share || per 0.1FTE || N/A || N/A || MIT only
|-
| Ingenuity Pathway Analysis || per dataset || N/A || $150.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || N/A || N/A ||
|-
| Tape Archive / Recovery || per tape || N/A || N/A ||
|-
| Informatics Training Classes || per session || N/A || N/A ||
|}

BioMicroCenter:Pricing

2026-04-16T19:43:13Z

Cstjohn: /* MiSeq i100 Assisted */

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,375.00 || $2,660.00
|-
| || One lane (~400M reads) || $1,250.00 || $1,400.00
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,312.50 || $3,710.00
|-
| || One lane (~400M reads) || $1,719.00 || $1,925.00
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,375.00 || $4,900.00
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 50 PE: F3 Lane (~100M reads) || $375.00 || $420.00
|-
| Rapid 300nt: F3 flowcell* || $2,250.00 || $2,520.00
|-
| 150 PE: F3 Lane (~100M reads) || $562.50 || $630.00
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit
|-
! Length !! Reads !! !!
|-
| 300nt || 5M || $893.75 || $1,001.00 || rowspan="9" | per flowcell
|-
| 600nt || 5M || $1,300.00 || $1,456.00
|-
| 100nt || 25M || $1,156.25 || $1,295.00
|-
| 300nt || 25M || $1,667.50 || $1,867.60
|-
| 600nt || 25M || $1,962.50 || $2,198.00
|-
| '''1000nt''' || 25M || $3,237.50 || $3,626.00
|-
| 100nt || 100M || $1,925.00 || $2,156.00
|-
| 300nt || 100M || $2,656.00 || $2,975.00
|-
| 600nt || 50M || $2,656.00 || $2,975.00
|}

==== MiSeq i100 Walkup ====

Walkup service is not available to external users.

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,650.00 || $1,848.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $1,787.50 || $2,002.00 ||
|-
| 300nt V2 kit (eg. 150PE) || $1,993.75 || $2,233.00 ||
|-
| 500nt V2 kit (eg. 250PE) || $2,200.00 || $2,464.00 ||
|-
| 600nt V3 kit (eg. 300PE) || $2,750.00 || $3,080.00 ||
|-
| 500nt '''NANO''' Run (eg. 250PE) || $962.50 || $1,078.00 ||
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $43.75 || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $18.75 || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $31.25 || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $500.00 || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| 72 hour Flowcell || $1,500.00 || $1,680.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| 72 hour Flowcell || colspan="2" | pricing in ilabs || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $37.50 || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $125.00 || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| LIGATION BASED NEB UltraII || STD || per sample || $136.50 || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. ||
|-
| HTL NEB UltraII || HTL || 24 || $1,125.00 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00 ||
|-
| Tagmentation Nextera-XT || STD || per Sample || $136.50 || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $204.00 || $235.00 ||
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,125.00 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00 ||
|-
| 16S Amplicon || HTL || 48 || $2,375.00 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $812.50 || $910.00 ||
|-
| Other Amplicon || HTL || 48 || $2,000.00 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || N/A || $945.00 ||
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00 ||
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $196.25 || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $293.75 || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $340.60 || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $273.00 || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $375.70 || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. ||
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,875.00 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,750.00 || $4,200.00 ||
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,312.50 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,625.00 || $2,940.00 ||
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,125.00 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00 ||
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,500.00 || $2,800.00 ||
|-
| || || 96 || $4,000.00 || $4,480.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,750.00 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00 ||
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00 ||
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Standard Ligation || LSK || $200.00 || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $65.00 || $70.00 ||
|-
| SMARTseq -> Ligation || Cv4+LSK || $375.00 || $450.00 ||
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $188.00 || $225.00 ||
|-
| Long Insert Prep || RAD (modified, target >100kb) || $340.60 || $393.00 ||
|-
| Direct RNA || RNA004 || $200.00 || $240.00 ||
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| DNA || 96 || $875.00 || $980.00 ||
|-
| RNA || 96 || $1,000.00 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $375.00 || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,250.00 || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="5" | '''Assisted Service''' ||
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION'' ||
|-
| 5' RNA / 3' RNA - setup || setup || $1,268.75 || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,188.00 || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,437.50 || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,881.00 || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,375.00 || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $337.50 || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $4,875.00 || $5,460.00 ||
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $587.50 || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $56.00 || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq'' ||
|-
| ATACseq - setup || setup || $1,100.00 || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,094.00 || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)'' ||
|-
| MULTI - setup || setup || $2,250.00 || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,875.00 || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION'' ||
|-
| per session || <24h notice || $250.00 || $280.00 ||
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Test Array || slide || $1,217.00 || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $7,510.00 || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $5,688.00 || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $9,813.00 || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $75.00 || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $60.00 || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Slide Only || per slide || $188.00 || $210.00 ||
|-
| Optimization Only || per slide || $1,000.00 || $1,120.00 ||
|-
| Teton Panel || per slide || $9,000.00 || $10,080.00 ||
|-
| Atlas Short || per slide || $6,875.00 || $7,700.00 ||
|-
| Antibody Add-on || per slide || $450.00 || $504.00 ||
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $18.75 || $21.00 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $100.00 || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $37.50 || $42.00 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $200.00 || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $62.50 || $70.00 ||
|-
| qPCR only (Short read quantification test) || per sample || $31.25 || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $500.00 || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:''' ||
|-
| Fresh Frozen Curl || per curl || $60.00 || $66.00 ||
|-
| FFPE Curl || per curl || $75.00 || $84.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Dr.Oligo || Assisted || $2,500.00 +consumables || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,250.00 +consumables || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,000.00 || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,250.00 || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $625.00 || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,875.00 || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $144.00 || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $375.00 || $420.00 ||
|-
| || add Quench || $125.00 || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to external users.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! SIGN UP !! Notes
|-
| colspan="6" | '''COVARIS SONICATOR''' ||
|-
| COVARIS E220 || $25.00 || $28.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="6" | '''PIPPIN PREP''' ||
|-
| Gel Isolation || $93.75 || $105.00 || per gel (5 lanes) || TBA ||
|-
| Training || N/A || $50.00 || per session || TBA || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="6" | '''PLATE READER''' ||
|-
| Varioskan || $23.00 || $27.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="6" | '''ROBOTICS''' ||
|-
| Tecan EVO 150 || $93.75 || $105.00 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| non-filter tips || $10.00 || $11.20 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $15.00 || $16.80 || per box || ||
|-
| Training || $81.25 || $91.00 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]] ||
|-
| colspan="6" | '''NANODROP''' ||
|-
| Nanodrop || -- || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Informatics Support || per hour || $180.00 || $205.00 || Very limited support for external users
|-
| Informatics Share || per 0.1FTE || N/A || N/A || MIT only
|-
| Ingenuity Pathway Analysis || per dataset || N/A || $150.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || N/A || N/A ||
|-
| Tape Archive / Recovery || per tape || N/A || N/A ||
|-
| Informatics Training Classes || per session || N/A || N/A ||
|}

BioMicroCenter:Pricing

2026-04-16T19:42:54Z

Cstjohn: /* MiSeq i100 Assisted */

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,375.00 || $2,660.00
|-
| || One lane (~400M reads) || $1,250.00 || $1,400.00
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,312.50 || $3,710.00
|-
| || One lane (~400M reads) || $1,719.00 || $1,925.00
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,375.00 || $4,900.00
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 50 PE: F3 Lane (~100M reads) || $375.00 || $420.00
|-
| Rapid 300nt: F3 flowcell* || $2,250.00 || $2,520.00
|-
| 150 PE: F3 Lane (~100M reads) || $562.50 || $630.00
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit
|-
! Length !! Reads
|-
| 300nt || 5M || $893.75 || $1,001.00 || rowspan="9" | per flowcell
|-
| 600nt || 5M || $1,300.00 || $1,456.00
|-
| 100nt || 25M || $1,156.25 || $1,295.00
|-
| 300nt || 25M || $1,667.50 || $1,867.60
|-
| 600nt || 25M || $1,962.50 || $2,198.00
|-
| '''1000nt''' || 25M || $3,237.50 || $3,626.00
|-
| 100nt || 100M || $1,925.00 || $2,156.00
|-
| 300nt || 100M || $2,656.00 || $2,975.00
|-
| 600nt || 50M || $2,656.00 || $2,975.00
|}

==== MiSeq i100 Walkup ====

Walkup service is not available to external users.

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,650.00 || $1,848.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $1,787.50 || $2,002.00 ||
|-
| 300nt V2 kit (eg. 150PE) || $1,993.75 || $2,233.00 ||
|-
| 500nt V2 kit (eg. 250PE) || $2,200.00 || $2,464.00 ||
|-
| 600nt V3 kit (eg. 300PE) || $2,750.00 || $3,080.00 ||
|-
| 500nt '''NANO''' Run (eg. 250PE) || $962.50 || $1,078.00 ||
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $43.75 || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $18.75 || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $31.25 || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $500.00 || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| 72 hour Flowcell || $1,500.00 || $1,680.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| 72 hour Flowcell || colspan="2" | pricing in ilabs || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $37.50 || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $125.00 || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| LIGATION BASED NEB UltraII || STD || per sample || $136.50 || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. ||
|-
| HTL NEB UltraII || HTL || 24 || $1,125.00 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00 ||
|-
| Tagmentation Nextera-XT || STD || per Sample || $136.50 || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $204.00 || $235.00 ||
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,125.00 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00 ||
|-
| 16S Amplicon || HTL || 48 || $2,375.00 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $812.50 || $910.00 ||
|-
| Other Amplicon || HTL || 48 || $2,000.00 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || N/A || $945.00 ||
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00 ||
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $196.25 || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $293.75 || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $340.60 || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $273.00 || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $375.70 || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. ||
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,875.00 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,750.00 || $4,200.00 ||
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,312.50 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,625.00 || $2,940.00 ||
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,125.00 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00 ||
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,500.00 || $2,800.00 ||
|-
| || || 96 || $4,000.00 || $4,480.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,750.00 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00 ||
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00 ||
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Standard Ligation || LSK || $200.00 || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $65.00 || $70.00 ||
|-
| SMARTseq -> Ligation || Cv4+LSK || $375.00 || $450.00 ||
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $188.00 || $225.00 ||
|-
| Long Insert Prep || RAD (modified, target >100kb) || $340.60 || $393.00 ||
|-
| Direct RNA || RNA004 || $200.00 || $240.00 ||
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| DNA || 96 || $875.00 || $980.00 ||
|-
| RNA || 96 || $1,000.00 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $375.00 || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,250.00 || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="5" | '''Assisted Service''' ||
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION'' ||
|-
| 5' RNA / 3' RNA - setup || setup || $1,268.75 || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,188.00 || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,437.50 || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,881.00 || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,375.00 || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $337.50 || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $4,875.00 || $5,460.00 ||
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $587.50 || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $56.00 || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq'' ||
|-
| ATACseq - setup || setup || $1,100.00 || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,094.00 || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)'' ||
|-
| MULTI - setup || setup || $2,250.00 || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,875.00 || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION'' ||
|-
| per session || <24h notice || $250.00 || $280.00 ||
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Test Array || slide || $1,217.00 || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $7,510.00 || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $5,688.00 || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $9,813.00 || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $75.00 || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $60.00 || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Slide Only || per slide || $188.00 || $210.00 ||
|-
| Optimization Only || per slide || $1,000.00 || $1,120.00 ||
|-
| Teton Panel || per slide || $9,000.00 || $10,080.00 ||
|-
| Atlas Short || per slide || $6,875.00 || $7,700.00 ||
|-
| Antibody Add-on || per slide || $450.00 || $504.00 ||
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $18.75 || $21.00 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $100.00 || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $37.50 || $42.00 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $200.00 || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $62.50 || $70.00 ||
|-
| qPCR only (Short read quantification test) || per sample || $31.25 || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $500.00 || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:''' ||
|-
| Fresh Frozen Curl || per curl || $60.00 || $66.00 ||
|-
| FFPE Curl || per curl || $75.00 || $84.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Dr.Oligo || Assisted || $2,500.00 +consumables || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,250.00 +consumables || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,000.00 || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,250.00 || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $625.00 || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,875.00 || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $144.00 || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $375.00 || $420.00 ||
|-
| || add Quench || $125.00 || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to external users.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! SIGN UP !! Notes
|-
| colspan="6" | '''COVARIS SONICATOR''' ||
|-
| COVARIS E220 || $25.00 || $28.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="6" | '''PIPPIN PREP''' ||
|-
| Gel Isolation || $93.75 || $105.00 || per gel (5 lanes) || TBA ||
|-
| Training || N/A || $50.00 || per session || TBA || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="6" | '''PLATE READER''' ||
|-
| Varioskan || $23.00 || $27.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="6" | '''ROBOTICS''' ||
|-
| Tecan EVO 150 || $93.75 || $105.00 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| non-filter tips || $10.00 || $11.20 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $15.00 || $16.80 || per box || ||
|-
| Training || $81.25 || $91.00 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]] ||
|-
| colspan="6" | '''NANODROP''' ||
|-
| Nanodrop || -- || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Informatics Support || per hour || $180.00 || $205.00 || Very limited support for external users
|-
| Informatics Share || per 0.1FTE || N/A || N/A || MIT only
|-
| Ingenuity Pathway Analysis || per dataset || N/A || $150.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || N/A || N/A ||
|-
| Tape Archive / Recovery || per tape || N/A || N/A ||
|-
| Informatics Training Classes || per session || N/A || N/A ||
|}

BioMicroCenter:Pricing

2026-04-16T19:42:43Z

Cstjohn: /* MiSeq i100 Assisted */

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,375.00 || $2,660.00
|-
| || One lane (~400M reads) || $1,250.00 || $1,400.00
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,312.50 || $3,710.00
|-
| || One lane (~400M reads) || $1,719.00 || $1,925.00
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,375.00 || $4,900.00
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 50 PE: F3 Lane (~100M reads) || $375.00 || $420.00
|-
| Rapid 300nt: F3 flowcell* || $2,250.00 || $2,520.00
|-
| 150 PE: F3 Lane (~100M reads) || $562.50 || $630.00
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit
|-
! Length !! Reads !! !! !! !!
|-
| 300nt || 5M || $893.75 || $1,001.00 || rowspan="9" | per flowcell
|-
| 600nt || 5M || $1,300.00 || $1,456.00
|-
| 100nt || 25M || $1,156.25 || $1,295.00
|-
| 300nt || 25M || $1,667.50 || $1,867.60
|-
| 600nt || 25M || $1,962.50 || $2,198.00
|-
| '''1000nt''' || 25M || $3,237.50 || $3,626.00
|-
| 100nt || 100M || $1,925.00 || $2,156.00
|-
| 300nt || 100M || $2,656.00 || $2,975.00
|-
| 600nt || 50M || $2,656.00 || $2,975.00
|}

==== MiSeq i100 Walkup ====

Walkup service is not available to external users.

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,650.00 || $1,848.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $1,787.50 || $2,002.00 ||
|-
| 300nt V2 kit (eg. 150PE) || $1,993.75 || $2,233.00 ||
|-
| 500nt V2 kit (eg. 250PE) || $2,200.00 || $2,464.00 ||
|-
| 600nt V3 kit (eg. 300PE) || $2,750.00 || $3,080.00 ||
|-
| 500nt '''NANO''' Run (eg. 250PE) || $962.50 || $1,078.00 ||
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $43.75 || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $18.75 || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $31.25 || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $500.00 || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| 72 hour Flowcell || $1,500.00 || $1,680.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| 72 hour Flowcell || colspan="2" | pricing in ilabs || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $37.50 || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $125.00 || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| LIGATION BASED NEB UltraII || STD || per sample || $136.50 || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. ||
|-
| HTL NEB UltraII || HTL || 24 || $1,125.00 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00 ||
|-
| Tagmentation Nextera-XT || STD || per Sample || $136.50 || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $204.00 || $235.00 ||
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,125.00 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00 ||
|-
| 16S Amplicon || HTL || 48 || $2,375.00 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $812.50 || $910.00 ||
|-
| Other Amplicon || HTL || 48 || $2,000.00 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || N/A || $945.00 ||
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00 ||
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $196.25 || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $293.75 || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $340.60 || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $273.00 || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $375.70 || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. ||
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,875.00 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,750.00 || $4,200.00 ||
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,312.50 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,625.00 || $2,940.00 ||
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,125.00 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00 ||
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,500.00 || $2,800.00 ||
|-
| || || 96 || $4,000.00 || $4,480.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,750.00 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00 ||
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00 ||
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Standard Ligation || LSK || $200.00 || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $65.00 || $70.00 ||
|-
| SMARTseq -> Ligation || Cv4+LSK || $375.00 || $450.00 ||
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $188.00 || $225.00 ||
|-
| Long Insert Prep || RAD (modified, target >100kb) || $340.60 || $393.00 ||
|-
| Direct RNA || RNA004 || $200.00 || $240.00 ||
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| DNA || 96 || $875.00 || $980.00 ||
|-
| RNA || 96 || $1,000.00 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $375.00 || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,250.00 || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="5" | '''Assisted Service''' ||
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION'' ||
|-
| 5' RNA / 3' RNA - setup || setup || $1,268.75 || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,188.00 || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,437.50 || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,881.00 || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,375.00 || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $337.50 || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $4,875.00 || $5,460.00 ||
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $587.50 || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $56.00 || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq'' ||
|-
| ATACseq - setup || setup || $1,100.00 || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,094.00 || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)'' ||
|-
| MULTI - setup || setup || $2,250.00 || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,875.00 || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION'' ||
|-
| per session || <24h notice || $250.00 || $280.00 ||
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Test Array || slide || $1,217.00 || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $7,510.00 || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $5,688.00 || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $9,813.00 || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $75.00 || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $60.00 || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Slide Only || per slide || $188.00 || $210.00 ||
|-
| Optimization Only || per slide || $1,000.00 || $1,120.00 ||
|-
| Teton Panel || per slide || $9,000.00 || $10,080.00 ||
|-
| Atlas Short || per slide || $6,875.00 || $7,700.00 ||
|-
| Antibody Add-on || per slide || $450.00 || $504.00 ||
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $18.75 || $21.00 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $100.00 || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $37.50 || $42.00 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $200.00 || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $62.50 || $70.00 ||
|-
| qPCR only (Short read quantification test) || per sample || $31.25 || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $500.00 || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:''' ||
|-
| Fresh Frozen Curl || per curl || $60.00 || $66.00 ||
|-
| FFPE Curl || per curl || $75.00 || $84.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Dr.Oligo || Assisted || $2,500.00 +consumables || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,250.00 +consumables || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,000.00 || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,250.00 || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $625.00 || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,875.00 || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $144.00 || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $375.00 || $420.00 ||
|-
| || add Quench || $125.00 || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to external users.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! SIGN UP !! Notes
|-
| colspan="6" | '''COVARIS SONICATOR''' ||
|-
| COVARIS E220 || $25.00 || $28.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="6" | '''PIPPIN PREP''' ||
|-
| Gel Isolation || $93.75 || $105.00 || per gel (5 lanes) || TBA ||
|-
| Training || N/A || $50.00 || per session || TBA || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="6" | '''PLATE READER''' ||
|-
| Varioskan || $23.00 || $27.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="6" | '''ROBOTICS''' ||
|-
| Tecan EVO 150 || $93.75 || $105.00 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| non-filter tips || $10.00 || $11.20 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $15.00 || $16.80 || per box || ||
|-
| Training || $81.25 || $91.00 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]] ||
|-
| colspan="6" | '''NANODROP''' ||
|-
| Nanodrop || -- || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Informatics Support || per hour || $180.00 || $205.00 || Very limited support for external users
|-
| Informatics Share || per 0.1FTE || N/A || N/A || MIT only
|-
| Ingenuity Pathway Analysis || per dataset || N/A || $150.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || N/A || N/A ||
|-
| Tape Archive / Recovery || per tape || N/A || N/A ||
|-
| Informatics Training Classes || per session || N/A || N/A ||
|}

BioMicroCenter:Pricing

2026-04-16T19:42:18Z

Cstjohn: /* MiSeq i100 Assisted */

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,375.00 || $2,660.00
|-
| || One lane (~400M reads) || $1,250.00 || $1,400.00
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,312.50 || $3,710.00
|-
| || One lane (~400M reads) || $1,719.00 || $1,925.00
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,375.00 || $4,900.00
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 50 PE: F3 Lane (~100M reads) || $375.00 || $420.00
|-
| Rapid 300nt: F3 flowcell* || $2,250.00 || $2,520.00
|-
| 150 PE: F3 Lane (~100M reads) || $562.50 || $630.00
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit
|-
! Length !! Reads !! !! !! !!
|-
| 300nt || 5M || $893.75 || $1,001.00 || rowspan="9" | per flowcell
|-
| 600nt || 5M || $1,300.00 || $1,456.00
|-
| 100nt || 25M || $1,156.25 || $1,295.00
|-
| 300nt || 25M || $1,667.50 || $1,867.60
|-
| 600nt || 25M || $1,962.50 || $2,198.00
|-
| '''1000nt''' || 25M || $3,237.50 || $3,626.00
|-
| 100nt || 100M || $1,925.00 || $2,156.00
|-
| 300nt || 100M || $2,656.00 || $2,975.00
|-
| 600nt || 50M || $2,656.00 || $2,975.00
|}

==== MiSeq i100 Walkup ====

Walkup service is not available to external users.

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,650.00 || $1,848.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $1,787.50 || $2,002.00 ||
|-
| 300nt V2 kit (eg. 150PE) || $1,993.75 || $2,233.00 ||
|-
| 500nt V2 kit (eg. 250PE) || $2,200.00 || $2,464.00 ||
|-
| 600nt V3 kit (eg. 300PE) || $2,750.00 || $3,080.00 ||
|-
| 500nt '''NANO''' Run (eg. 250PE) || $962.50 || $1,078.00 ||
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $43.75 || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $18.75 || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $31.25 || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $500.00 || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| 72 hour Flowcell || $1,500.00 || $1,680.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| 72 hour Flowcell || colspan="2" | pricing in ilabs || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $37.50 || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $125.00 || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| LIGATION BASED NEB UltraII || STD || per sample || $136.50 || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. ||
|-
| HTL NEB UltraII || HTL || 24 || $1,125.00 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00 ||
|-
| Tagmentation Nextera-XT || STD || per Sample || $136.50 || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $204.00 || $235.00 ||
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,125.00 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00 ||
|-
| 16S Amplicon || HTL || 48 || $2,375.00 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $812.50 || $910.00 ||
|-
| Other Amplicon || HTL || 48 || $2,000.00 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || N/A || $945.00 ||
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00 ||
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $196.25 || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $293.75 || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $340.60 || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $273.00 || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $375.70 || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. ||
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,875.00 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,750.00 || $4,200.00 ||
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,312.50 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,625.00 || $2,940.00 ||
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,125.00 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00 ||
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,500.00 || $2,800.00 ||
|-
| || || 96 || $4,000.00 || $4,480.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,750.00 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00 ||
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00 ||
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Standard Ligation || LSK || $200.00 || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $65.00 || $70.00 ||
|-
| SMARTseq -> Ligation || Cv4+LSK || $375.00 || $450.00 ||
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $188.00 || $225.00 ||
|-
| Long Insert Prep || RAD (modified, target >100kb) || $340.60 || $393.00 ||
|-
| Direct RNA || RNA004 || $200.00 || $240.00 ||
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| DNA || 96 || $875.00 || $980.00 ||
|-
| RNA || 96 || $1,000.00 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $375.00 || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,250.00 || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="5" | '''Assisted Service''' ||
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION'' ||
|-
| 5' RNA / 3' RNA - setup || setup || $1,268.75 || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,188.00 || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,437.50 || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,881.00 || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,375.00 || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $337.50 || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $4,875.00 || $5,460.00 ||
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $587.50 || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $56.00 || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq'' ||
|-
| ATACseq - setup || setup || $1,100.00 || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,094.00 || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)'' ||
|-
| MULTI - setup || setup || $2,250.00 || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,875.00 || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION'' ||
|-
| per session || <24h notice || $250.00 || $280.00 ||
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Test Array || slide || $1,217.00 || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $7,510.00 || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $5,688.00 || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $9,813.00 || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $75.00 || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $60.00 || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Slide Only || per slide || $188.00 || $210.00 ||
|-
| Optimization Only || per slide || $1,000.00 || $1,120.00 ||
|-
| Teton Panel || per slide || $9,000.00 || $10,080.00 ||
|-
| Atlas Short || per slide || $6,875.00 || $7,700.00 ||
|-
| Antibody Add-on || per slide || $450.00 || $504.00 ||
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $18.75 || $21.00 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $100.00 || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $37.50 || $42.00 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $200.00 || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $62.50 || $70.00 ||
|-
| qPCR only (Short read quantification test) || per sample || $31.25 || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $500.00 || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:''' ||
|-
| Fresh Frozen Curl || per curl || $60.00 || $66.00 ||
|-
| FFPE Curl || per curl || $75.00 || $84.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Dr.Oligo || Assisted || $2,500.00 +consumables || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,250.00 +consumables || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,000.00 || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,250.00 || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $625.00 || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,875.00 || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $144.00 || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $375.00 || $420.00 ||
|-
| || add Quench || $125.00 || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to external users.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! SIGN UP !! Notes
|-
| colspan="6" | '''COVARIS SONICATOR''' ||
|-
| COVARIS E220 || $25.00 || $28.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="6" | '''PIPPIN PREP''' ||
|-
| Gel Isolation || $93.75 || $105.00 || per gel (5 lanes) || TBA ||
|-
| Training || N/A || $50.00 || per session || TBA || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="6" | '''PLATE READER''' ||
|-
| Varioskan || $23.00 || $27.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="6" | '''ROBOTICS''' ||
|-
| Tecan EVO 150 || $93.75 || $105.00 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| non-filter tips || $10.00 || $11.20 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $15.00 || $16.80 || per box || ||
|-
| Training || $81.25 || $91.00 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]] ||
|-
| colspan="6" | '''NANODROP''' ||
|-
| Nanodrop || -- || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Informatics Support || per hour || $180.00 || $205.00 || Very limited support for external users
|-
| Informatics Share || per 0.1FTE || N/A || N/A || MIT only
|-
| Ingenuity Pathway Analysis || per dataset || N/A || $150.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || N/A || N/A ||
|-
| Tape Archive / Recovery || per tape || N/A || N/A ||
|-
| Informatics Training Classes || per session || N/A || N/A ||
|}

BioMicroCenter:Pricing

2026-04-16T19:42:06Z

Cstjohn: /* MiSeq i100 Assisted */

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,375.00 || $2,660.00
|-
| || One lane (~400M reads) || $1,250.00 || $1,400.00
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,312.50 || $3,710.00
|-
| || One lane (~400M reads) || $1,719.00 || $1,925.00
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,375.00 || $4,900.00
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 50 PE: F3 Lane (~100M reads) || $375.00 || $420.00
|-
| Rapid 300nt: F3 flowcell* || $2,250.00 || $2,520.00
|-
| 150 PE: F3 Lane (~100M reads) || $562.50 || $630.00
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
! Length !! Reads !! !! !! !!
|-
| 300nt || 5M || $893.75 || $1,001.00 || rowspan="9" | per flowcell
|-
| 600nt || 5M || $1,300.00 || $1,456.00
|-
| 100nt || 25M || $1,156.25 || $1,295.00
|-
| 300nt || 25M || $1,667.50 || $1,867.60
|-
| 600nt || 25M || $1,962.50 || $2,198.00
|-
| '''1000nt''' || 25M || $3,237.50 || $3,626.00
|-
| 100nt || 100M || $1,925.00 || $2,156.00
|-
| 300nt || 100M || $2,656.00 || $2,975.00
|-
| 600nt || 50M || $2,656.00 || $2,975.00
|}

==== MiSeq i100 Walkup ====

Walkup service is not available to external users.

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,650.00 || $1,848.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $1,787.50 || $2,002.00 ||
|-
| 300nt V2 kit (eg. 150PE) || $1,993.75 || $2,233.00 ||
|-
| 500nt V2 kit (eg. 250PE) || $2,200.00 || $2,464.00 ||
|-
| 600nt V3 kit (eg. 300PE) || $2,750.00 || $3,080.00 ||
|-
| 500nt '''NANO''' Run (eg. 250PE) || $962.50 || $1,078.00 ||
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $43.75 || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $18.75 || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $31.25 || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $500.00 || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| 72 hour Flowcell || $1,500.00 || $1,680.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| 72 hour Flowcell || colspan="2" | pricing in ilabs || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $37.50 || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $125.00 || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| LIGATION BASED NEB UltraII || STD || per sample || $136.50 || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. ||
|-
| HTL NEB UltraII || HTL || 24 || $1,125.00 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00 ||
|-
| Tagmentation Nextera-XT || STD || per Sample || $136.50 || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $204.00 || $235.00 ||
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,125.00 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00 ||
|-
| 16S Amplicon || HTL || 48 || $2,375.00 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $812.50 || $910.00 ||
|-
| Other Amplicon || HTL || 48 || $2,000.00 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || N/A || $945.00 ||
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00 ||
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $196.25 || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $293.75 || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $340.60 || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $273.00 || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $375.70 || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. ||
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,875.00 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,750.00 || $4,200.00 ||
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,312.50 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,625.00 || $2,940.00 ||
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,125.00 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00 ||
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,500.00 || $2,800.00 ||
|-
| || || 96 || $4,000.00 || $4,480.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,750.00 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00 ||
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00 ||
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Standard Ligation || LSK || $200.00 || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $65.00 || $70.00 ||
|-
| SMARTseq -> Ligation || Cv4+LSK || $375.00 || $450.00 ||
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $188.00 || $225.00 ||
|-
| Long Insert Prep || RAD (modified, target >100kb) || $340.60 || $393.00 ||
|-
| Direct RNA || RNA004 || $200.00 || $240.00 ||
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| DNA || 96 || $875.00 || $980.00 ||
|-
| RNA || 96 || $1,000.00 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $375.00 || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,250.00 || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="5" | '''Assisted Service''' ||
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION'' ||
|-
| 5' RNA / 3' RNA - setup || setup || $1,268.75 || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,188.00 || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,437.50 || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,881.00 || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,375.00 || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $337.50 || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $4,875.00 || $5,460.00 ||
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $587.50 || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $56.00 || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq'' ||
|-
| ATACseq - setup || setup || $1,100.00 || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,094.00 || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)'' ||
|-
| MULTI - setup || setup || $2,250.00 || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,875.00 || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION'' ||
|-
| per session || <24h notice || $250.00 || $280.00 ||
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Test Array || slide || $1,217.00 || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $7,510.00 || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $5,688.00 || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $9,813.00 || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $75.00 || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $60.00 || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Slide Only || per slide || $188.00 || $210.00 ||
|-
| Optimization Only || per slide || $1,000.00 || $1,120.00 ||
|-
| Teton Panel || per slide || $9,000.00 || $10,080.00 ||
|-
| Atlas Short || per slide || $6,875.00 || $7,700.00 ||
|-
| Antibody Add-on || per slide || $450.00 || $504.00 ||
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $18.75 || $21.00 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $100.00 || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $37.50 || $42.00 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $200.00 || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $62.50 || $70.00 ||
|-
| qPCR only (Short read quantification test) || per sample || $31.25 || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $500.00 || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:''' ||
|-
| Fresh Frozen Curl || per curl || $60.00 || $66.00 ||
|-
| FFPE Curl || per curl || $75.00 || $84.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Dr.Oligo || Assisted || $2,500.00 +consumables || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,250.00 +consumables || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,000.00 || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,250.00 || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $625.00 || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,875.00 || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $144.00 || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $375.00 || $420.00 ||
|-
| || add Quench || $125.00 || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to external users.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! SIGN UP !! Notes
|-
| colspan="6" | '''COVARIS SONICATOR''' ||
|-
| COVARIS E220 || $25.00 || $28.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="6" | '''PIPPIN PREP''' ||
|-
| Gel Isolation || $93.75 || $105.00 || per gel (5 lanes) || TBA ||
|-
| Training || N/A || $50.00 || per session || TBA || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="6" | '''PLATE READER''' ||
|-
| Varioskan || $23.00 || $27.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="6" | '''ROBOTICS''' ||
|-
| Tecan EVO 150 || $93.75 || $105.00 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| non-filter tips || $10.00 || $11.20 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $15.00 || $16.80 || per box || ||
|-
| Training || $81.25 || $91.00 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]] ||
|-
| colspan="6" | '''NANODROP''' ||
|-
| Nanodrop || -- || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Informatics Support || per hour || $180.00 || $205.00 || Very limited support for external users
|-
| Informatics Share || per 0.1FTE || N/A || N/A || MIT only
|-
| Ingenuity Pathway Analysis || per dataset || N/A || $150.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || N/A || N/A ||
|-
| Tape Archive / Recovery || per tape || N/A || N/A ||
|-
| Informatics Training Classes || per session || N/A || N/A ||
|}

BioMicroCenter:Pricing

2026-04-16T19:40:59Z

Cstjohn: /* SINGULAR G4 */

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,375.00 || $2,660.00
|-
| || One lane (~400M reads) || $1,250.00 || $1,400.00
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,312.50 || $3,710.00
|-
| || One lane (~400M reads) || $1,719.00 || $1,925.00
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,375.00 || $4,900.00
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 50 PE: F3 Lane (~100M reads) || $375.00 || $420.00
|-
| Rapid 300nt: F3 flowcell* || $2,250.00 || $2,520.00
|-
| 150 PE: F3 Lane (~100M reads) || $562.50 || $630.00
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
! Length !! Reads !! !! !! !!
|-
| 300nt || 5M || $893.75 || $1,001.00 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,300.00 || $1,456.00 ||
|-
| 100nt || 25M || $1,156.25 || $1,295.00 ||
|-
| 300nt || 25M || $1,667.50 || $1,867.60 ||
|-
| 600nt || 25M || $1,962.50 || $2,198.00 ||
|-
| '''1000nt''' || 25M || $3,237.50 || $3,626.00 ||
|-
| 100nt || 100M || $1,925.00 || $2,156.00 ||
|-
| 300nt || 100M || $2,656.00 || $2,975.00 ||
|-
| 600nt || 50M || $2,656.00 || $2,975.00 ||
|}

==== MiSeq i100 Walkup ====

Walkup service is not available to external users.

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,650.00 || $1,848.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $1,787.50 || $2,002.00 ||
|-
| 300nt V2 kit (eg. 150PE) || $1,993.75 || $2,233.00 ||
|-
| 500nt V2 kit (eg. 250PE) || $2,200.00 || $2,464.00 ||
|-
| 600nt V3 kit (eg. 300PE) || $2,750.00 || $3,080.00 ||
|-
| 500nt '''NANO''' Run (eg. 250PE) || $962.50 || $1,078.00 ||
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $43.75 || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $18.75 || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $31.25 || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $500.00 || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| 72 hour Flowcell || $1,500.00 || $1,680.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| 72 hour Flowcell || colspan="2" | pricing in ilabs || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $37.50 || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $125.00 || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| LIGATION BASED NEB UltraII || STD || per sample || $136.50 || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. ||
|-
| HTL NEB UltraII || HTL || 24 || $1,125.00 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00 ||
|-
| Tagmentation Nextera-XT || STD || per Sample || $136.50 || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $204.00 || $235.00 ||
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,125.00 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00 ||
|-
| 16S Amplicon || HTL || 48 || $2,375.00 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $812.50 || $910.00 ||
|-
| Other Amplicon || HTL || 48 || $2,000.00 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || N/A || $945.00 ||
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00 ||
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $196.25 || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $293.75 || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $340.60 || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $273.00 || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $375.70 || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. ||
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,875.00 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,750.00 || $4,200.00 ||
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,312.50 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,625.00 || $2,940.00 ||
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,125.00 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00 ||
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,500.00 || $2,800.00 ||
|-
| || || 96 || $4,000.00 || $4,480.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,750.00 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00 ||
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00 ||
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Standard Ligation || LSK || $200.00 || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $65.00 || $70.00 ||
|-
| SMARTseq -> Ligation || Cv4+LSK || $375.00 || $450.00 ||
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $188.00 || $225.00 ||
|-
| Long Insert Prep || RAD (modified, target >100kb) || $340.60 || $393.00 ||
|-
| Direct RNA || RNA004 || $200.00 || $240.00 ||
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| DNA || 96 || $875.00 || $980.00 ||
|-
| RNA || 96 || $1,000.00 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $375.00 || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,250.00 || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="5" | '''Assisted Service''' ||
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION'' ||
|-
| 5' RNA / 3' RNA - setup || setup || $1,268.75 || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,188.00 || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,437.50 || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,881.00 || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,375.00 || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $337.50 || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $4,875.00 || $5,460.00 ||
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $587.50 || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $56.00 || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq'' ||
|-
| ATACseq - setup || setup || $1,100.00 || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,094.00 || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)'' ||
|-
| MULTI - setup || setup || $2,250.00 || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,875.00 || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION'' ||
|-
| per session || <24h notice || $250.00 || $280.00 ||
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Test Array || slide || $1,217.00 || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $7,510.00 || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $5,688.00 || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $9,813.00 || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $75.00 || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $60.00 || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Slide Only || per slide || $188.00 || $210.00 ||
|-
| Optimization Only || per slide || $1,000.00 || $1,120.00 ||
|-
| Teton Panel || per slide || $9,000.00 || $10,080.00 ||
|-
| Atlas Short || per slide || $6,875.00 || $7,700.00 ||
|-
| Antibody Add-on || per slide || $450.00 || $504.00 ||
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $18.75 || $21.00 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $100.00 || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $37.50 || $42.00 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $200.00 || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $62.50 || $70.00 ||
|-
| qPCR only (Short read quantification test) || per sample || $31.25 || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $500.00 || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:''' ||
|-
| Fresh Frozen Curl || per curl || $60.00 || $66.00 ||
|-
| FFPE Curl || per curl || $75.00 || $84.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Dr.Oligo || Assisted || $2,500.00 +consumables || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,250.00 +consumables || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,000.00 || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,250.00 || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $625.00 || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,875.00 || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $144.00 || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $375.00 || $420.00 ||
|-
| || add Quench || $125.00 || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to external users.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! SIGN UP !! Notes
|-
| colspan="6" | '''COVARIS SONICATOR''' ||
|-
| COVARIS E220 || $25.00 || $28.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="6" | '''PIPPIN PREP''' ||
|-
| Gel Isolation || $93.75 || $105.00 || per gel (5 lanes) || TBA ||
|-
| Training || N/A || $50.00 || per session || TBA || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="6" | '''PLATE READER''' ||
|-
| Varioskan || $23.00 || $27.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="6" | '''ROBOTICS''' ||
|-
| Tecan EVO 150 || $93.75 || $105.00 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| non-filter tips || $10.00 || $11.20 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $15.00 || $16.80 || per box || ||
|-
| Training || $81.25 || $91.00 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]] ||
|-
| colspan="6" | '''NANODROP''' ||
|-
| Nanodrop || -- || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Informatics Support || per hour || $180.00 || $205.00 || Very limited support for external users
|-
| Informatics Share || per 0.1FTE || N/A || N/A || MIT only
|-
| Ingenuity Pathway Analysis || per dataset || N/A || $150.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || N/A || N/A ||
|-
| Tape Archive / Recovery || per tape || N/A || N/A ||
|-
| Informatics Training Classes || per session || N/A || N/A ||
|}

BioMicroCenter:Pricing

2026-04-16T19:40:44Z

Cstjohn: /* SINGULAR G4 */

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,375.00 || $2,660.00
|-
| || One lane (~400M reads) || $1,250.00 || $1,400.00
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,312.50 || $3,710.00
|-
| || One lane (~400M reads) || $1,719.00 || $1,925.00
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,375.00 || $4,900.00
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $375.00 || $420.00
|-
| Rapid 300nt: F3 flowcell* || $2,250.00 || $2,520.00
|-
| 150 PE: F3 Lane (~100M reads) || $562.50 || $630.00
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
! Length !! Reads !! !! !! !!
|-
| 300nt || 5M || $893.75 || $1,001.00 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,300.00 || $1,456.00 ||
|-
| 100nt || 25M || $1,156.25 || $1,295.00 ||
|-
| 300nt || 25M || $1,667.50 || $1,867.60 ||
|-
| 600nt || 25M || $1,962.50 || $2,198.00 ||
|-
| '''1000nt''' || 25M || $3,237.50 || $3,626.00 ||
|-
| 100nt || 100M || $1,925.00 || $2,156.00 ||
|-
| 300nt || 100M || $2,656.00 || $2,975.00 ||
|-
| 600nt || 50M || $2,656.00 || $2,975.00 ||
|}

==== MiSeq i100 Walkup ====

Walkup service is not available to external users.

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,650.00 || $1,848.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $1,787.50 || $2,002.00 ||
|-
| 300nt V2 kit (eg. 150PE) || $1,993.75 || $2,233.00 ||
|-
| 500nt V2 kit (eg. 250PE) || $2,200.00 || $2,464.00 ||
|-
| 600nt V3 kit (eg. 300PE) || $2,750.00 || $3,080.00 ||
|-
| 500nt '''NANO''' Run (eg. 250PE) || $962.50 || $1,078.00 ||
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $43.75 || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $18.75 || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $31.25 || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $500.00 || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| 72 hour Flowcell || $1,500.00 || $1,680.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| 72 hour Flowcell || colspan="2" | pricing in ilabs || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $37.50 || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $125.00 || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| LIGATION BASED NEB UltraII || STD || per sample || $136.50 || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. ||
|-
| HTL NEB UltraII || HTL || 24 || $1,125.00 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00 ||
|-
| Tagmentation Nextera-XT || STD || per Sample || $136.50 || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $204.00 || $235.00 ||
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,125.00 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00 ||
|-
| 16S Amplicon || HTL || 48 || $2,375.00 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $812.50 || $910.00 ||
|-
| Other Amplicon || HTL || 48 || $2,000.00 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || N/A || $945.00 ||
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00 ||
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $196.25 || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $293.75 || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $340.60 || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $273.00 || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $375.70 || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. ||
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,875.00 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,750.00 || $4,200.00 ||
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,312.50 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,625.00 || $2,940.00 ||
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,125.00 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00 ||
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,500.00 || $2,800.00 ||
|-
| || || 96 || $4,000.00 || $4,480.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,750.00 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00 ||
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00 ||
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Standard Ligation || LSK || $200.00 || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $65.00 || $70.00 ||
|-
| SMARTseq -> Ligation || Cv4+LSK || $375.00 || $450.00 ||
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $188.00 || $225.00 ||
|-
| Long Insert Prep || RAD (modified, target >100kb) || $340.60 || $393.00 ||
|-
| Direct RNA || RNA004 || $200.00 || $240.00 ||
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| DNA || 96 || $875.00 || $980.00 ||
|-
| RNA || 96 || $1,000.00 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $375.00 || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,250.00 || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="5" | '''Assisted Service''' ||
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION'' ||
|-
| 5' RNA / 3' RNA - setup || setup || $1,268.75 || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,188.00 || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,437.50 || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,881.00 || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,375.00 || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $337.50 || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $4,875.00 || $5,460.00 ||
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $587.50 || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $56.00 || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq'' ||
|-
| ATACseq - setup || setup || $1,100.00 || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,094.00 || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)'' ||
|-
| MULTI - setup || setup || $2,250.00 || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,875.00 || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION'' ||
|-
| per session || <24h notice || $250.00 || $280.00 ||
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Test Array || slide || $1,217.00 || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $7,510.00 || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $5,688.00 || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $9,813.00 || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $75.00 || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $60.00 || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Slide Only || per slide || $188.00 || $210.00 ||
|-
| Optimization Only || per slide || $1,000.00 || $1,120.00 ||
|-
| Teton Panel || per slide || $9,000.00 || $10,080.00 ||
|-
| Atlas Short || per slide || $6,875.00 || $7,700.00 ||
|-
| Antibody Add-on || per slide || $450.00 || $504.00 ||
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $18.75 || $21.00 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $100.00 || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $37.50 || $42.00 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $200.00 || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $62.50 || $70.00 ||
|-
| qPCR only (Short read quantification test) || per sample || $31.25 || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $500.00 || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:''' ||
|-
| Fresh Frozen Curl || per curl || $60.00 || $66.00 ||
|-
| FFPE Curl || per curl || $75.00 || $84.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Dr.Oligo || Assisted || $2,500.00 +consumables || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,250.00 +consumables || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,000.00 || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,250.00 || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $625.00 || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,875.00 || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $144.00 || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $375.00 || $420.00 ||
|-
| || add Quench || $125.00 || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to external users.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! SIGN UP !! Notes
|-
| colspan="6" | '''COVARIS SONICATOR''' ||
|-
| COVARIS E220 || $25.00 || $28.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="6" | '''PIPPIN PREP''' ||
|-
| Gel Isolation || $93.75 || $105.00 || per gel (5 lanes) || TBA ||
|-
| Training || N/A || $50.00 || per session || TBA || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="6" | '''PLATE READER''' ||
|-
| Varioskan || $23.00 || $27.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="6" | '''ROBOTICS''' ||
|-
| Tecan EVO 150 || $93.75 || $105.00 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| non-filter tips || $10.00 || $11.20 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $15.00 || $16.80 || per box || ||
|-
| Training || $81.25 || $91.00 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]] ||
|-
| colspan="6" | '''NANODROP''' ||
|-
| Nanodrop || -- || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Informatics Support || per hour || $180.00 || $205.00 || Very limited support for external users
|-
| Informatics Share || per 0.1FTE || N/A || N/A || MIT only
|-
| Ingenuity Pathway Analysis || per dataset || N/A || $150.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || N/A || N/A ||
|-
| Tape Archive / Recovery || per tape || N/A || N/A ||
|-
| Informatics Training Classes || per session || N/A || N/A ||
|}

BioMicroCenter:Pricing

2026-04-16T19:39:44Z

Cstjohn: /* ELEMENT AVITI24 */

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,375.00 || $2,660.00
|-
| || One lane (~400M reads) || $1,250.00 || $1,400.00
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,312.50 || $3,710.00
|-
| || One lane (~400M reads) || $1,719.00 || $1,925.00
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,375.00 || $4,900.00
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $375.00 || $420.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $2,250.00 || $2,520.00 || per flowcell ||
|-
| 150 PE: F3 Lane (~100M reads) || $562.50 || $630.00 || per lane ||
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
! Length !! Reads !! !! !! !!
|-
| 300nt || 5M || $893.75 || $1,001.00 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,300.00 || $1,456.00 ||
|-
| 100nt || 25M || $1,156.25 || $1,295.00 ||
|-
| 300nt || 25M || $1,667.50 || $1,867.60 ||
|-
| 600nt || 25M || $1,962.50 || $2,198.00 ||
|-
| '''1000nt''' || 25M || $3,237.50 || $3,626.00 ||
|-
| 100nt || 100M || $1,925.00 || $2,156.00 ||
|-
| 300nt || 100M || $2,656.00 || $2,975.00 ||
|-
| 600nt || 50M || $2,656.00 || $2,975.00 ||
|}

==== MiSeq i100 Walkup ====

Walkup service is not available to external users.

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,650.00 || $1,848.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $1,787.50 || $2,002.00 ||
|-
| 300nt V2 kit (eg. 150PE) || $1,993.75 || $2,233.00 ||
|-
| 500nt V2 kit (eg. 250PE) || $2,200.00 || $2,464.00 ||
|-
| 600nt V3 kit (eg. 300PE) || $2,750.00 || $3,080.00 ||
|-
| 500nt '''NANO''' Run (eg. 250PE) || $962.50 || $1,078.00 ||
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $43.75 || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $18.75 || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $31.25 || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $500.00 || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| 72 hour Flowcell || $1,500.00 || $1,680.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| 72 hour Flowcell || colspan="2" | pricing in ilabs || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $37.50 || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $125.00 || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| LIGATION BASED NEB UltraII || STD || per sample || $136.50 || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. ||
|-
| HTL NEB UltraII || HTL || 24 || $1,125.00 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00 ||
|-
| Tagmentation Nextera-XT || STD || per Sample || $136.50 || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $204.00 || $235.00 ||
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,125.00 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00 ||
|-
| 16S Amplicon || HTL || 48 || $2,375.00 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $812.50 || $910.00 ||
|-
| Other Amplicon || HTL || 48 || $2,000.00 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || N/A || $945.00 ||
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00 ||
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $196.25 || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $293.75 || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $340.60 || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $273.00 || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $375.70 || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. ||
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,875.00 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,750.00 || $4,200.00 ||
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,312.50 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,625.00 || $2,940.00 ||
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,125.00 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00 ||
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,500.00 || $2,800.00 ||
|-
| || || 96 || $4,000.00 || $4,480.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,750.00 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00 ||
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00 ||
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Standard Ligation || LSK || $200.00 || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $65.00 || $70.00 ||
|-
| SMARTseq -> Ligation || Cv4+LSK || $375.00 || $450.00 ||
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $188.00 || $225.00 ||
|-
| Long Insert Prep || RAD (modified, target >100kb) || $340.60 || $393.00 ||
|-
| Direct RNA || RNA004 || $200.00 || $240.00 ||
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| DNA || 96 || $875.00 || $980.00 ||
|-
| RNA || 96 || $1,000.00 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $375.00 || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,250.00 || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="5" | '''Assisted Service''' ||
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION'' ||
|-
| 5' RNA / 3' RNA - setup || setup || $1,268.75 || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,188.00 || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,437.50 || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,881.00 || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,375.00 || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $337.50 || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $4,875.00 || $5,460.00 ||
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $587.50 || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $56.00 || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq'' ||
|-
| ATACseq - setup || setup || $1,100.00 || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,094.00 || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)'' ||
|-
| MULTI - setup || setup || $2,250.00 || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,875.00 || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION'' ||
|-
| per session || <24h notice || $250.00 || $280.00 ||
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Test Array || slide || $1,217.00 || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $7,510.00 || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $5,688.00 || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $9,813.00 || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $75.00 || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $60.00 || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Slide Only || per slide || $188.00 || $210.00 ||
|-
| Optimization Only || per slide || $1,000.00 || $1,120.00 ||
|-
| Teton Panel || per slide || $9,000.00 || $10,080.00 ||
|-
| Atlas Short || per slide || $6,875.00 || $7,700.00 ||
|-
| Antibody Add-on || per slide || $450.00 || $504.00 ||
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $18.75 || $21.00 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $100.00 || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $37.50 || $42.00 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $200.00 || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $62.50 || $70.00 ||
|-
| qPCR only (Short read quantification test) || per sample || $31.25 || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $500.00 || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:''' ||
|-
| Fresh Frozen Curl || per curl || $60.00 || $66.00 ||
|-
| FFPE Curl || per curl || $75.00 || $84.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Dr.Oligo || Assisted || $2,500.00 +consumables || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,250.00 +consumables || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,000.00 || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,250.00 || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $625.00 || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,875.00 || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $144.00 || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $375.00 || $420.00 ||
|-
| || add Quench || $125.00 || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to external users.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! SIGN UP !! Notes
|-
| colspan="6" | '''COVARIS SONICATOR''' ||
|-
| COVARIS E220 || $25.00 || $28.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="6" | '''PIPPIN PREP''' ||
|-
| Gel Isolation || $93.75 || $105.00 || per gel (5 lanes) || TBA ||
|-
| Training || N/A || $50.00 || per session || TBA || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="6" | '''PLATE READER''' ||
|-
| Varioskan || $23.00 || $27.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="6" | '''ROBOTICS''' ||
|-
| Tecan EVO 150 || $93.75 || $105.00 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| non-filter tips || $10.00 || $11.20 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $15.00 || $16.80 || per box || ||
|-
| Training || $81.25 || $91.00 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]] ||
|-
| colspan="6" | '''NANODROP''' ||
|-
| Nanodrop || -- || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Informatics Support || per hour || $180.00 || $205.00 || Very limited support for external users
|-
| Informatics Share || per 0.1FTE || N/A || N/A || MIT only
|-
| Ingenuity Pathway Analysis || per dataset || N/A || $150.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || N/A || N/A ||
|-
| Tape Archive / Recovery || per tape || N/A || N/A ||
|-
| Informatics Training Classes || per session || N/A || N/A ||
|}

BioMicroCenter:Pricing

2026-04-16T19:38:28Z

Cstjohn: /* ELEMENT AVITI24 */

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,375.00 || $2,660.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,250.00 || $1,400.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,312.50 || $3,710.00 || per flowcell
|-
| || One lane (~400M reads) || $1,719.00 || $1,925.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,375.00 || $4,900.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $375.00 || $420.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $2,250.00 || $2,520.00 || per flowcell ||
|-
| 150 PE: F3 Lane (~100M reads) || $562.50 || $630.00 || per lane ||
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
! Length !! Reads !! !! !! !!
|-
| 300nt || 5M || $893.75 || $1,001.00 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,300.00 || $1,456.00 ||
|-
| 100nt || 25M || $1,156.25 || $1,295.00 ||
|-
| 300nt || 25M || $1,667.50 || $1,867.60 ||
|-
| 600nt || 25M || $1,962.50 || $2,198.00 ||
|-
| '''1000nt''' || 25M || $3,237.50 || $3,626.00 ||
|-
| 100nt || 100M || $1,925.00 || $2,156.00 ||
|-
| 300nt || 100M || $2,656.00 || $2,975.00 ||
|-
| 600nt || 50M || $2,656.00 || $2,975.00 ||
|}

==== MiSeq i100 Walkup ====

Walkup service is not available to external users.

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,650.00 || $1,848.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $1,787.50 || $2,002.00 ||
|-
| 300nt V2 kit (eg. 150PE) || $1,993.75 || $2,233.00 ||
|-
| 500nt V2 kit (eg. 250PE) || $2,200.00 || $2,464.00 ||
|-
| 600nt V3 kit (eg. 300PE) || $2,750.00 || $3,080.00 ||
|-
| 500nt '''NANO''' Run (eg. 250PE) || $962.50 || $1,078.00 ||
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $43.75 || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $18.75 || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $31.25 || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $500.00 || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| 72 hour Flowcell || $1,500.00 || $1,680.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| 72 hour Flowcell || colspan="2" | pricing in ilabs || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $37.50 || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $125.00 || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| LIGATION BASED NEB UltraII || STD || per sample || $136.50 || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. ||
|-
| HTL NEB UltraII || HTL || 24 || $1,125.00 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00 ||
|-
| Tagmentation Nextera-XT || STD || per Sample || $136.50 || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $204.00 || $235.00 ||
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,125.00 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00 ||
|-
| 16S Amplicon || HTL || 48 || $2,375.00 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $812.50 || $910.00 ||
|-
| Other Amplicon || HTL || 48 || $2,000.00 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || N/A || $945.00 ||
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00 ||
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $196.25 || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $293.75 || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $340.60 || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $273.00 || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $375.70 || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. ||
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,875.00 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,750.00 || $4,200.00 ||
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,312.50 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,625.00 || $2,940.00 ||
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,125.00 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00 ||
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,500.00 || $2,800.00 ||
|-
| || || 96 || $4,000.00 || $4,480.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,750.00 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00 ||
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00 ||
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Standard Ligation || LSK || $200.00 || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $65.00 || $70.00 ||
|-
| SMARTseq -> Ligation || Cv4+LSK || $375.00 || $450.00 ||
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $188.00 || $225.00 ||
|-
| Long Insert Prep || RAD (modified, target >100kb) || $340.60 || $393.00 ||
|-
| Direct RNA || RNA004 || $200.00 || $240.00 ||
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| DNA || 96 || $875.00 || $980.00 ||
|-
| RNA || 96 || $1,000.00 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $375.00 || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,250.00 || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="5" | '''Assisted Service''' ||
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION'' ||
|-
| 5' RNA / 3' RNA - setup || setup || $1,268.75 || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,188.00 || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,437.50 || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,881.00 || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,375.00 || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $337.50 || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $4,875.00 || $5,460.00 ||
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $587.50 || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $56.00 || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq'' ||
|-
| ATACseq - setup || setup || $1,100.00 || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,094.00 || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)'' ||
|-
| MULTI - setup || setup || $2,250.00 || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,875.00 || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION'' ||
|-
| per session || <24h notice || $250.00 || $280.00 ||
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Test Array || slide || $1,217.00 || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $7,510.00 || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $5,688.00 || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $9,813.00 || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $75.00 || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $60.00 || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Slide Only || per slide || $188.00 || $210.00 ||
|-
| Optimization Only || per slide || $1,000.00 || $1,120.00 ||
|-
| Teton Panel || per slide || $9,000.00 || $10,080.00 ||
|-
| Atlas Short || per slide || $6,875.00 || $7,700.00 ||
|-
| Antibody Add-on || per slide || $450.00 || $504.00 ||
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $18.75 || $21.00 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $100.00 || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $37.50 || $42.00 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $200.00 || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $62.50 || $70.00 ||
|-
| qPCR only (Short read quantification test) || per sample || $31.25 || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $500.00 || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:''' ||
|-
| Fresh Frozen Curl || per curl || $60.00 || $66.00 ||
|-
| FFPE Curl || per curl || $75.00 || $84.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Dr.Oligo || Assisted || $2,500.00 +consumables || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,250.00 +consumables || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,000.00 || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,250.00 || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $625.00 || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,875.00 || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $144.00 || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $375.00 || $420.00 ||
|-
| || add Quench || $125.00 || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to external users.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! SIGN UP !! Notes
|-
| colspan="6" | '''COVARIS SONICATOR''' ||
|-
| COVARIS E220 || $25.00 || $28.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="6" | '''PIPPIN PREP''' ||
|-
| Gel Isolation || $93.75 || $105.00 || per gel (5 lanes) || TBA ||
|-
| Training || N/A || $50.00 || per session || TBA || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="6" | '''PLATE READER''' ||
|-
| Varioskan || $23.00 || $27.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="6" | '''ROBOTICS''' ||
|-
| Tecan EVO 150 || $93.75 || $105.00 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| non-filter tips || $10.00 || $11.20 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $15.00 || $16.80 || per box || ||
|-
| Training || $81.25 || $91.00 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]] ||
|-
| colspan="6" | '''NANODROP''' ||
|-
| Nanodrop || -- || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Informatics Support || per hour || $180.00 || $205.00 || Very limited support for external users
|-
| Informatics Share || per 0.1FTE || N/A || N/A || MIT only
|-
| Ingenuity Pathway Analysis || per dataset || N/A || $150.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || N/A || N/A ||
|-
| Tape Archive / Recovery || per tape || N/A || N/A ||
|-
| Informatics Training Classes || per session || N/A || N/A ||
|}

BioMicroCenter:Pricing

2026-04-15T17:51:47Z

Cstjohn:

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,375.00 || $2,660.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,250.00 || $1,400.00 || per lane ||
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,312.50 || $3,710.00 || per flowcell ||
|-
| || One lane (~400M reads) || $1,719.00 || $1,925.00 || per lane ||
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,375.00 || $4,900.00 || per lane ||
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $375.00 || $420.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $2,250.00 || $2,520.00 || per flowcell ||
|-
| 150 PE: F3 Lane (~100M reads) || $562.50 || $630.00 || per lane ||
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
! Length !! Reads !! !! !! !!
|-
| 300nt || 5M || $893.75 || $1,001.00 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,300.00 || $1,456.00 ||
|-
| 100nt || 25M || $1,156.25 || $1,295.00 ||
|-
| 300nt || 25M || $1,667.50 || $1,867.60 ||
|-
| 600nt || 25M || $1,962.50 || $2,198.00 ||
|-
| '''1000nt''' || 25M || $3,237.50 || $3,626.00 ||
|-
| 100nt || 100M || $1,925.00 || $2,156.00 ||
|-
| 300nt || 100M || $2,656.00 || $2,975.00 ||
|-
| 600nt || 50M || $2,656.00 || $2,975.00 ||
|}

==== MiSeq i100 Walkup ====

Walkup service is not available to external users.

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,650.00 || $1,848.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $1,787.50 || $2,002.00 ||
|-
| 300nt V2 kit (eg. 150PE) || $1,993.75 || $2,233.00 ||
|-
| 500nt V2 kit (eg. 250PE) || $2,200.00 || $2,464.00 ||
|-
| 600nt V3 kit (eg. 300PE) || $2,750.00 || $3,080.00 ||
|-
| 500nt '''NANO''' Run (eg. 250PE) || $962.50 || $1,078.00 ||
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $43.75 || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $18.75 || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $31.25 || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $500.00 || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| 72 hour Flowcell || $1,500.00 || $1,680.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
| 72 hour Flowcell || colspan="2" | pricing in ilabs || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $37.50 || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $125.00 || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| LIGATION BASED NEB UltraII || STD || per sample || $136.50 || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="6" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. ||
|-
| HTL NEB UltraII || HTL || 24 || $1,125.00 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00 ||
|-
| Tagmentation Nextera-XT || STD || per Sample || $136.50 || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $204.00 || $235.00 ||
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,125.00 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,250.00 || $2,520.00 ||
|-
| 16S Amplicon || HTL || 48 || $2,375.00 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $812.50 || $910.00 ||
|-
| Other Amplicon || HTL || 48 || $2,000.00 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || N/A || $945.00 ||
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00 ||
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $196.25 || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $293.75 || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $340.60 || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $273.00 || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $375.70 || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. ||
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,875.00 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $3,750.00 || $4,200.00 ||
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,312.50 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,625.00 || $2,940.00 ||
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,125.00 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00 ||
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,500.00 || $2,800.00 ||
|-
| || || 96 || $4,000.00 || $4,480.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,750.00 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $6,250.00 || $7,000.00 ||
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00 ||
|-
| Sample Cleanup || || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Standard Ligation || LSK || $200.00 || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $65.00 || $70.00 ||
|-
| SMARTseq -> Ligation || Cv4+LSK || $375.00 || $450.00 ||
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $188.00 || $225.00 ||
|-
| Long Insert Prep || RAD (modified, target >100kb) || $340.60 || $393.00 ||
|-
| Direct RNA || RNA004 || $200.00 || $240.00 ||
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| DNA || 96 || $875.00 || $980.00 ||
|-
| RNA || 96 || $1,000.00 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $375.00 || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,250.00 || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| colspan="5" | '''Assisted Service''' ||
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION'' ||
|-
| 5' RNA / 3' RNA - setup || setup || $1,268.75 || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,188.00 || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,437.50 || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,881.00 || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,375.00 || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $337.50 || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $4,875.00 || $5,460.00 ||
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $587.50 || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $56.00 || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''scATACseq'' ||
|-
| ATACseq - setup || setup || $1,100.00 || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,094.00 || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)'' ||
|-
| MULTI - setup || setup || $2,250.00 || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $3,875.00 || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="5" | ''CANCELLATION'' ||
|-
| per session || <24h notice || $250.00 || $280.00 ||
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Test Array || slide || $1,217.00 || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $7,510.00 || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $5,688.00 || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $9,813.00 || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $75.00 || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $60.00 || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Slide Only || per slide || $188.00 || $210.00 ||
|-
| Optimization Only || per slide || $1,000.00 || $1,120.00 ||
|-
| Teton Panel || per slide || $9,000.00 || $10,080.00 ||
|-
| Atlas Short || per slide || $6,875.00 || $7,700.00 ||
|-
| Antibody Add-on || per slide || $450.00 || $504.00 ||
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $18.75 || $21.00 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $100.00 || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $37.50 || $42.00 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $200.00 || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $62.50 || $70.00 ||
|-
| qPCR only (Short read quantification test) || per sample || $31.25 || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $500.00 || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="5" | '''Sample QC services:''' ||
|-
| Fresh Frozen Curl || per curl || $60.00 || $66.00 ||
|-
| FFPE Curl || per curl || $75.00 || $84.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Dr.Oligo || Assisted || $2,500.00 +consumables || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,250.00 +consumables || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,000.00 || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,250.00 || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $625.00 || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $1,875.00 || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $144.00 || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $375.00 || $420.00 ||
|-
| || add Quench || $125.00 || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to external users.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! SIGN UP !! Notes
|-
| colspan="6" | '''COVARIS SONICATOR''' ||
|-
| COVARIS E220 || $25.00 || $28.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="6" | '''PIPPIN PREP''' ||
|-
| Gel Isolation || $93.75 || $105.00 || per gel (5 lanes) || TBA ||
|-
| Training || N/A || $50.00 || per session || TBA || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="6" | '''PLATE READER''' ||
|-
| Varioskan || $23.00 || $27.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="6" | '''ROBOTICS''' ||
|-
| Tecan EVO 150 || $93.75 || $105.00 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| non-filter tips || $10.00 || $11.20 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $15.00 || $16.80 || per box || ||
|-
| Training || $81.25 || $91.00 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]] ||
|-
| colspan="6" | '''NANODROP''' ||
|-
| Nanodrop || -- || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
| Informatics Support || per hour || $180.00 || $205.00 || Very limited support for external users
|-
| Informatics Share || per 0.1FTE || N/A || N/A || MIT only
|-
| Ingenuity Pathway Analysis || per dataset || N/A || $150.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || N/A || N/A ||
|-
| Tape Archive / Recovery || per tape || N/A || N/A ||
|-
| Informatics Training Classes || per session || N/A || N/A ||
|}

BioMicroCenter:Pricing

2026-04-15T17:42:39Z

Cstjohn:

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,660.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,400.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,710.00 || per flowcell
|-
| || One lane (~400M reads) || $1,925.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,900.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $420.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $2,520.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $630.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
! Length !! Reads !! !! !!
|-
| 300nt || 5M || $1,001.00 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,456.00
|-
| 100nt || 25M || $1,295.00
|-
| 300nt || 25M || $1,867.60
|-
| 600nt || 25M || $2,198.00
|-
| '''1000nt''' || 25M || $3,626.00
|-
| 100nt || 100M || $2,156.00
|-
| 300nt || 100M || $2,975.00
|-
| 600nt || 50M || $2,975.00
|}


==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,848.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $2,002.00
|-
| 300nt V2 kit (eg. 150PE) || $2,233.00
|-
| 500nt V2 kit (eg. 250PE) || $2,464.00
|-
| 600nt V3 kit (eg. 300PE) || $3,080.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $1,078.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 72 hour Flowcell || $1,680.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 72 hour Flowcell || pricing in ilabs || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="5" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="5" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,520.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $235.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,520.00
|-
| 16S Amplicon || HTL || 48 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $910.00
|-
| Other Amplicon || HTL || 48 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $945.00
|-
| colspan="5" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $84.00
|-
| Sample Cleanup || || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="5" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="5" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $4,200.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,940.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $7,000.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,800.00
|-
| || || 96 || $4,480.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $7,000.00
|-
| colspan="5" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $84.00
|-
| Sample Cleanup || || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Standard Ligation || LSK || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $70.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $450.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $225.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $393.00
|-
| Direct RNA || RNA004 || $240.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
|-
| DNA || 96 || $980.00
|-
| RNA || 96 || $1,120.00
|-
| RNA/DNA Isolation - Assisted || 12 samples || $420.00
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,400.00
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="4" | '''Assisted Service'''
|-
| colspan="4" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $5,460.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="4" | ''scATACseq''
|-
| ATACseq - setup || setup || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="4" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="4" | ''CANCELLATION''
|-
| per session || <24h notice || $280.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Test Array || slide || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! SPATIAL (per slide) !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
|-
| Slide Only || $210.00
|-
| Optimization Only || $1,120.00
|-
| Teton Panel || $10,080.00
|-
| Atlas Short || $7,700.00
|-
| Antibody Add-on || $504.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $21.00
|-
| Fragment Analyzer: Preloaded row || per row (12) || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $42.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $70.00
|-
| qPCR only (Short read quantification test) || per sample || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="4" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $66.00
|-
| FFPE Curl || per curl || $84.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Dr.Oligo || Assisted || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $420.00 ||
|-
| || add Quench || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to Non-MIT users.

== OTHER LAB SERVICES ==

Email biomicro@mit.edu for more information.

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $28.00 || per hour
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $105.00 || per gel (5 lanes)
|-
| Training || $50.00 || per session
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $27.00 || per hour
|-
| colspan="5" | '''LIQUID HANDLING'''
|-
| Tecan EVO 150 || $105.00 || per 1h block - based on scheduled time.
|-
| non-filter tips || $11.20 || per box
|-
| Filter tips || $16.80 || per box
|-
| Training || $91.00 || per hour
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Informatics Support || per hour || $205.00 || Very limited support for non-MIT users
|-
| Ingenuity Pathway Analysis || per dataset || $150.00 || Recovery of licence cost.
|}

BioMicroCenter:Pricing

2026-04-15T17:40:44Z

Cstjohn: /* CHEMAGIC360 */

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,660.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,400.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,710.00 || per flowcell
|-
| || One lane (~400M reads) || $1,925.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,900.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $420.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $2,520.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $630.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
! Length !! Reads !! !! !!
|-
| 300nt || 5M || $1,001.00 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,456.00
|-
| 100nt || 25M || $1,295.00
|-
| 300nt || 25M || $1,867.60
|-
| 600nt || 25M || $2,198.00
|-
| '''1000nt''' || 25M || $3,626.00
|-
| 100nt || 100M || $2,156.00
|-
| 300nt || 100M || $2,975.00
|-
| 600nt || 50M || $2,975.00
|}


==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,848.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $2,002.00
|-
| 300nt V2 kit (eg. 150PE) || $2,233.00
|-
| 500nt V2 kit (eg. 250PE) || $2,464.00
|-
| 600nt V3 kit (eg. 300PE) || $3,080.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $1,078.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 72 hour Flowcell || $1,680.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 72 hour Flowcell || pricing in ilabs || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="5" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="5" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,520.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $235.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,520.00
|-
| 16S Amplicon || HTL || 48 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $910.00
|-
| Other Amplicon || HTL || 48 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $945.00
|-
| colspan="5" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $84.00
|-
| Sample Cleanup || || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="5" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="5" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $4,200.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,940.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $7,000.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,800.00
|-
| || || 96 || $4,480.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $7,000.00
|-
| colspan="5" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $84.00
|-
| Sample Cleanup || || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Standard Ligation || LSK || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $70.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $450.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $225.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $393.00
|-
| Direct RNA || RNA004 || $240.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
|-
| DNA || 96 || $980.00
|-
| RNA || 96 || $1,120.00
|-
| RNA/DNA Isolation - Assisted || 12 samples || $420.00
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,400.00
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="4" | '''Assisted Service'''
|-
| colspan="4" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $5,460.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="4" | ''scATACseq''
|-
| ATACseq - setup || setup || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="4" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="4" | ''CANCELLATION''
|-
| per session || <24h notice || $280.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Test Array || slide || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! SPATIAL (per slide) !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
|-
| Slide Only || $210.00
|-
| Optimization Only || $1,120.00
|-
| Teton Panel || $10,080.00
|-
| Atlas Short || $7,700.00
|-
| Antibody Add-on || $504.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $21.00
|-
| Fragment Analyzer: Preloaded row || per row (12) || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $42.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $70.00
|-
| qPCR only (Short read quantification test) || per sample || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="4" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $66.00
|-
| FFPE Curl || per curl || $84.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Dr.Oligo || Assisted || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $420.00 ||
|-
| || add Quench || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to Non-MIT users.

== OTHER LAB SERVICES ==

Email biomicro@mit.edu for more information.

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $28.00 || per hour
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $105.00 || per gel (5 lanes)
|-
| Training || $50.00 || per session
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $27.00 || per hour
|-
| colspan="5" | '''LIQUID HANDLING'''
|-
| Tecan EVO 150 || $105.00 || per 1h block - based on scheduled time.
|-
| non-filter tips || $11.20 || per box
|-
| Filter tips || $16.80 || per box
|-
| Training || $91.00 || per hour
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Informatics Support || per hour || $205.00 || Very limited support for non-MIT users
|-
| Ingenuity Pathway Analysis || per dataset || $150.00 || Recovery of licence cost.
|}

BioMicroCenter:Pricing

2026-04-15T17:37:58Z

Cstjohn: /* ELEMENT AVITI24 */

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,660.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,400.00 || per lane
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,710.00 || per flowcell
|-
| || One lane (~400M reads) || $1,925.00 || per lane
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,900.00 || per lane
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $420.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $2,520.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $630.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
! Length !! Reads !! !! !!
|-
| 300nt || 5M || $1,001.00 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,456.00
|-
| 100nt || 25M || $1,295.00
|-
| 300nt || 25M || $1,867.60
|-
| 600nt || 25M || $2,198.00
|-
| '''1000nt''' || 25M || $3,626.00
|-
| 100nt || 100M || $2,156.00
|-
| 300nt || 100M || $2,975.00
|-
| 600nt || 50M || $2,975.00
|}


==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,848.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $2,002.00
|-
| 300nt V2 kit (eg. 150PE) || $2,233.00
|-
| 500nt V2 kit (eg. 250PE) || $2,464.00
|-
| 600nt V3 kit (eg. 300PE) || $3,080.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $1,078.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 72 hour Flowcell || $1,680.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 72 hour Flowcell || pricing in ilabs || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="5" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="5" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,520.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $235.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,520.00
|-
| 16S Amplicon || HTL || 48 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $910.00
|-
| Other Amplicon || HTL || 48 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $945.00
|-
| colspan="5" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $84.00
|-
| Sample Cleanup || || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="5" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="5" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $4,200.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,940.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $7,000.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,800.00
|-
| || || 96 || $4,480.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $7,000.00
|-
| colspan="5" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $84.00
|-
| Sample Cleanup || || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Standard Ligation || LSK || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $70.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $450.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $225.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $393.00
|-
| Direct RNA || RNA004 || $240.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| DNA || 96 || $980.00 ||
|-
| RNA || 96 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="4" | '''Assisted Service'''
|-
| colspan="4" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $5,460.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="4" | ''scATACseq''
|-
| ATACseq - setup || setup || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="4" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="4" | ''CANCELLATION''
|-
| per session || <24h notice || $280.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Test Array || slide || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! SPATIAL (per slide) !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
|-
| Slide Only || $210.00
|-
| Optimization Only || $1,120.00
|-
| Teton Panel || $10,080.00
|-
| Atlas Short || $7,700.00
|-
| Antibody Add-on || $504.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $21.00
|-
| Fragment Analyzer: Preloaded row || per row (12) || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $42.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $70.00
|-
| qPCR only (Short read quantification test) || per sample || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="4" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $66.00
|-
| FFPE Curl || per curl || $84.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Dr.Oligo || Assisted || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $420.00 ||
|-
| || add Quench || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to Non-MIT users.

== OTHER LAB SERVICES ==

Email biomicro@mit.edu for more information.

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $28.00 || per hour
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $105.00 || per gel (5 lanes)
|-
| Training || $50.00 || per session
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $27.00 || per hour
|-
| colspan="5" | '''LIQUID HANDLING'''
|-
| Tecan EVO 150 || $105.00 || per 1h block - based on scheduled time.
|-
| non-filter tips || $11.20 || per box
|-
| Filter tips || $16.80 || per box
|-
| Training || $91.00 || per hour
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Informatics Support || per hour || $205.00 || Very limited support for non-MIT users
|-
| Ingenuity Pathway Analysis || per dataset || $150.00 || Recovery of licence cost.
|}

BioMicroCenter:Pricing

2026-04-15T17:37:41Z

Cstjohn: /* SINGULAR G4 */

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,660.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,400.00 || per lane ||
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,710.00 || per flowcell ||
|-
| || One lane (~400M reads) || $1,925.00 || per lane ||
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,900.00 || per lane ||
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $420.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $2,520.00 || per flowcell
|-
| 150 PE: F3 Lane (~100M reads) || $630.00 || per lane
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
! Length !! Reads !! !! !!
|-
| 300nt || 5M || $1,001.00 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,456.00
|-
| 100nt || 25M || $1,295.00
|-
| 300nt || 25M || $1,867.60
|-
| 600nt || 25M || $2,198.00
|-
| '''1000nt''' || 25M || $3,626.00
|-
| 100nt || 100M || $2,156.00
|-
| 300nt || 100M || $2,975.00
|-
| 600nt || 50M || $2,975.00
|}


==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,848.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $2,002.00
|-
| 300nt V2 kit (eg. 150PE) || $2,233.00
|-
| 500nt V2 kit (eg. 250PE) || $2,464.00
|-
| 600nt V3 kit (eg. 300PE) || $3,080.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $1,078.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 72 hour Flowcell || $1,680.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 72 hour Flowcell || pricing in ilabs || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="5" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="5" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,520.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $235.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,520.00
|-
| 16S Amplicon || HTL || 48 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $910.00
|-
| Other Amplicon || HTL || 48 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $945.00
|-
| colspan="5" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $84.00
|-
| Sample Cleanup || || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="5" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="5" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $4,200.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,940.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $7,000.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,800.00
|-
| || || 96 || $4,480.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $7,000.00
|-
| colspan="5" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $84.00
|-
| Sample Cleanup || || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Standard Ligation || LSK || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $70.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $450.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $225.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $393.00
|-
| Direct RNA || RNA004 || $240.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| DNA || 96 || $980.00 ||
|-
| RNA || 96 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="4" | '''Assisted Service'''
|-
| colspan="4" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $5,460.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="4" | ''scATACseq''
|-
| ATACseq - setup || setup || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="4" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="4" | ''CANCELLATION''
|-
| per session || <24h notice || $280.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Test Array || slide || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! SPATIAL (per slide) !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
|-
| Slide Only || $210.00
|-
| Optimization Only || $1,120.00
|-
| Teton Panel || $10,080.00
|-
| Atlas Short || $7,700.00
|-
| Antibody Add-on || $504.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $21.00
|-
| Fragment Analyzer: Preloaded row || per row (12) || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $42.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $70.00
|-
| qPCR only (Short read quantification test) || per sample || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="4" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $66.00
|-
| FFPE Curl || per curl || $84.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Dr.Oligo || Assisted || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $420.00 ||
|-
| || add Quench || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to Non-MIT users.

== OTHER LAB SERVICES ==

Email biomicro@mit.edu for more information.

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $28.00 || per hour
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $105.00 || per gel (5 lanes)
|-
| Training || $50.00 || per session
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $27.00 || per hour
|-
| colspan="5" | '''LIQUID HANDLING'''
|-
| Tecan EVO 150 || $105.00 || per 1h block - based on scheduled time.
|-
| non-filter tips || $11.20 || per box
|-
| Filter tips || $16.80 || per box
|-
| Training || $91.00 || per hour
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Informatics Support || per hour || $205.00 || Very limited support for non-MIT users
|-
| Ingenuity Pathway Analysis || per dataset || $150.00 || Recovery of licence cost.
|}

BioMicroCenter:Pricing

2026-04-15T17:37:29Z

Cstjohn: /* MISEQ SEQUENCING */

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,660.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,400.00 || per lane ||
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,710.00 || per flowcell ||
|-
| || One lane (~400M reads) || $1,925.00 || per lane ||
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,900.00 || per lane ||
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $420.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $2,520.00 || per flowcell ||
|-
| 150 PE: F3 Lane (~100M reads) || $630.00 || per lane ||
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
! Length !! Reads !! !! !!
|-
| 300nt || 5M || $1,001.00 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,456.00
|-
| 100nt || 25M || $1,295.00
|-
| 300nt || 25M || $1,867.60
|-
| 600nt || 25M || $2,198.00
|-
| '''1000nt''' || 25M || $3,626.00
|-
| 100nt || 100M || $2,156.00
|-
| 300nt || 100M || $2,975.00
|-
| 600nt || 50M || $2,975.00
|}


==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,848.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $2,002.00
|-
| 300nt V2 kit (eg. 150PE) || $2,233.00
|-
| 500nt V2 kit (eg. 250PE) || $2,464.00
|-
| 600nt V3 kit (eg. 300PE) || $3,080.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $1,078.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 72 hour Flowcell || $1,680.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 72 hour Flowcell || pricing in ilabs || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="5" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="5" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,520.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $235.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,520.00
|-
| 16S Amplicon || HTL || 48 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $910.00
|-
| Other Amplicon || HTL || 48 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $945.00
|-
| colspan="5" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $84.00
|-
| Sample Cleanup || || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="5" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="5" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $4,200.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,940.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $7,000.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,800.00
|-
| || || 96 || $4,480.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $7,000.00
|-
| colspan="5" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $84.00
|-
| Sample Cleanup || || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Standard Ligation || LSK || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $70.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $450.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $225.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $393.00
|-
| Direct RNA || RNA004 || $240.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| DNA || 96 || $980.00 ||
|-
| RNA || 96 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="4" | '''Assisted Service'''
|-
| colspan="4" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $5,460.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="4" | ''scATACseq''
|-
| ATACseq - setup || setup || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="4" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="4" | ''CANCELLATION''
|-
| per session || <24h notice || $280.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Test Array || slide || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! SPATIAL (per slide) !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
|-
| Slide Only || $210.00
|-
| Optimization Only || $1,120.00
|-
| Teton Panel || $10,080.00
|-
| Atlas Short || $7,700.00
|-
| Antibody Add-on || $504.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $21.00
|-
| Fragment Analyzer: Preloaded row || per row (12) || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $42.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $70.00
|-
| qPCR only (Short read quantification test) || per sample || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="4" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $66.00
|-
| FFPE Curl || per curl || $84.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Dr.Oligo || Assisted || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $420.00 ||
|-
| || add Quench || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to Non-MIT users.

== OTHER LAB SERVICES ==

Email biomicro@mit.edu for more information.

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $28.00 || per hour
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $105.00 || per gel (5 lanes)
|-
| Training || $50.00 || per session
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $27.00 || per hour
|-
| colspan="5" | '''LIQUID HANDLING'''
|-
| Tecan EVO 150 || $105.00 || per 1h block - based on scheduled time.
|-
| non-filter tips || $11.20 || per box
|-
| Filter tips || $16.80 || per box
|-
| Training || $91.00 || per hour
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Informatics Support || per hour || $205.00 || Very limited support for non-MIT users
|-
| Ingenuity Pathway Analysis || per dataset || $150.00 || Recovery of licence cost.
|}

BioMicroCenter:Pricing

2026-04-15T17:36:59Z

Cstjohn: /* MiSeq i100 Assisted */

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,660.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,400.00 || per lane ||
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,710.00 || per flowcell ||
|-
| || One lane (~400M reads) || $1,925.00 || per lane ||
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,900.00 || per lane ||
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $420.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $2,520.00 || per flowcell ||
|-
| 150 PE: F3 Lane (~100M reads) || $630.00 || per lane ||
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
! Length !! Reads !! !! !!
|-
| 300nt || 5M || $1,001.00 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,456.00
|-
| 100nt || 25M || $1,295.00
|-
| 300nt || 25M || $1,867.60
|-
| 600nt || 25M || $2,198.00
|-
| '''1000nt''' || 25M || $3,626.00
|-
| 100nt || 100M || $2,156.00
|-
| 300nt || 100M || $2,975.00
|-
| 600nt || 50M || $2,975.00
|}

==== MiSeq i100 Walkup ====

Walkup service is not available to Non-MIT users.

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,848.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $2,002.00
|-
| 300nt V2 kit (eg. 150PE) || $2,233.00
|-
| 500nt V2 kit (eg. 250PE) || $2,464.00
|-
| 600nt V3 kit (eg. 300PE) || $3,080.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $1,078.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 72 hour Flowcell || $1,680.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 72 hour Flowcell || pricing in ilabs || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="5" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="5" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,520.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $235.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,520.00
|-
| 16S Amplicon || HTL || 48 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $910.00
|-
| Other Amplicon || HTL || 48 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $945.00
|-
| colspan="5" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $84.00
|-
| Sample Cleanup || || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="5" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="5" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $4,200.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,940.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $7,000.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,800.00
|-
| || || 96 || $4,480.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $7,000.00
|-
| colspan="5" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $84.00
|-
| Sample Cleanup || || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Standard Ligation || LSK || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $70.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $450.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $225.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $393.00
|-
| Direct RNA || RNA004 || $240.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| DNA || 96 || $980.00 ||
|-
| RNA || 96 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="4" | '''Assisted Service'''
|-
| colspan="4" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $5,460.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="4" | ''scATACseq''
|-
| ATACseq - setup || setup || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="4" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="4" | ''CANCELLATION''
|-
| per session || <24h notice || $280.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Test Array || slide || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! SPATIAL (per slide) !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
|-
| Slide Only || $210.00
|-
| Optimization Only || $1,120.00
|-
| Teton Panel || $10,080.00
|-
| Atlas Short || $7,700.00
|-
| Antibody Add-on || $504.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $21.00
|-
| Fragment Analyzer: Preloaded row || per row (12) || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $42.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $70.00
|-
| qPCR only (Short read quantification test) || per sample || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="4" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $66.00
|-
| FFPE Curl || per curl || $84.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Dr.Oligo || Assisted || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $420.00 ||
|-
| || add Quench || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to Non-MIT users.

== OTHER LAB SERVICES ==

Email biomicro@mit.edu for more information.

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $28.00 || per hour
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $105.00 || per gel (5 lanes)
|-
| Training || $50.00 || per session
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $27.00 || per hour
|-
| colspan="5" | '''LIQUID HANDLING'''
|-
| Tecan EVO 150 || $105.00 || per 1h block - based on scheduled time.
|-
| non-filter tips || $11.20 || per box
|-
| Filter tips || $16.80 || per box
|-
| Training || $91.00 || per hour
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Informatics Support || per hour || $205.00 || Very limited support for non-MIT users
|-
| Ingenuity Pathway Analysis || per dataset || $150.00 || Recovery of licence cost.
|}

BioMicroCenter:Pricing

2026-04-15T17:36:40Z

Cstjohn: /* MiSeq classic */

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,660.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,400.00 || per lane ||
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,710.00 || per flowcell ||
|-
| || One lane (~400M reads) || $1,925.00 || per lane ||
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,900.00 || per lane ||
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $420.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $2,520.00 || per flowcell ||
|-
| 150 PE: F3 Lane (~100M reads) || $630.00 || per lane ||
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
! Length !! Reads !! !! !!
|-
| 300nt || 5M || $1,001.00 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,456.00 ||
|-
| 100nt || 25M || $1,295.00 ||
|-
| 300nt || 25M || $1,867.60 ||
|-
| 600nt || 25M || $2,198.00 ||
|-
| '''1000nt''' || 25M || $3,626.00 ||
|-
| 100nt || 100M || $2,156.00 ||
|-
| 300nt || 100M || $2,975.00 ||
|-
| 600nt || 50M || $2,975.00 ||
|}

==== MiSeq i100 Walkup ====

Walkup service is not available to Non-MIT users.

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,848.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $2,002.00
|-
| 300nt V2 kit (eg. 150PE) || $2,233.00
|-
| 500nt V2 kit (eg. 250PE) || $2,464.00
|-
| 600nt V3 kit (eg. 300PE) || $3,080.00
|-
| 500nt '''NANO''' Run (eg. 250PE) || $1,078.00
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 72 hour Flowcell || $1,680.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 72 hour Flowcell || pricing in ilabs || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="5" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="5" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,520.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $235.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,520.00
|-
| 16S Amplicon || HTL || 48 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $910.00
|-
| Other Amplicon || HTL || 48 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $945.00
|-
| colspan="5" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $84.00
|-
| Sample Cleanup || || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="5" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="5" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $4,200.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,940.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $7,000.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,800.00
|-
| || || 96 || $4,480.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $7,000.00
|-
| colspan="5" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $84.00
|-
| Sample Cleanup || || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Standard Ligation || LSK || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $70.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $450.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $225.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $393.00
|-
| Direct RNA || RNA004 || $240.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| DNA || 96 || $980.00 ||
|-
| RNA || 96 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="4" | '''Assisted Service'''
|-
| colspan="4" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $5,460.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="4" | ''scATACseq''
|-
| ATACseq - setup || setup || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="4" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="4" | ''CANCELLATION''
|-
| per session || <24h notice || $280.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Test Array || slide || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! SPATIAL (per slide) !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
|-
| Slide Only || $210.00
|-
| Optimization Only || $1,120.00
|-
| Teton Panel || $10,080.00
|-
| Atlas Short || $7,700.00
|-
| Antibody Add-on || $504.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $21.00
|-
| Fragment Analyzer: Preloaded row || per row (12) || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $42.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $70.00
|-
| qPCR only (Short read quantification test) || per sample || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="4" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $66.00
|-
| FFPE Curl || per curl || $84.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Dr.Oligo || Assisted || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $420.00 ||
|-
| || add Quench || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to Non-MIT users.

== OTHER LAB SERVICES ==

Email biomicro@mit.edu for more information.

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $28.00 || per hour
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $105.00 || per gel (5 lanes)
|-
| Training || $50.00 || per session
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $27.00 || per hour
|-
| colspan="5" | '''LIQUID HANDLING'''
|-
| Tecan EVO 150 || $105.00 || per 1h block - based on scheduled time.
|-
| non-filter tips || $11.20 || per box
|-
| Filter tips || $16.80 || per box
|-
| Training || $91.00 || per hour
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Informatics Support || per hour || $205.00 || Very limited support for non-MIT users
|-
| Ingenuity Pathway Analysis || per dataset || $150.00 || Recovery of licence cost.
|}

BioMicroCenter:Pricing

2026-04-15T17:36:02Z

Cstjohn: /* SHORT READ LIBRARY - DNA */

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,660.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,400.00 || per lane ||
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,710.00 || per flowcell ||
|-
| || One lane (~400M reads) || $1,925.00 || per lane ||
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,900.00 || per lane ||
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $420.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $2,520.00 || per flowcell ||
|-
| 150 PE: F3 Lane (~100M reads) || $630.00 || per lane ||
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
! Length !! Reads !! !! !!
|-
| 300nt || 5M || $1,001.00 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,456.00 ||
|-
| 100nt || 25M || $1,295.00 ||
|-
| 300nt || 25M || $1,867.60 ||
|-
| 600nt || 25M || $2,198.00 ||
|-
| '''1000nt''' || 25M || $3,626.00 ||
|-
| 100nt || 100M || $2,156.00 ||
|-
| 300nt || 100M || $2,975.00 ||
|-
| 600nt || 50M || $2,975.00 ||
|}

==== MiSeq i100 Walkup ====

Walkup service is not available to Non-MIT users.

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,848.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $2,002.00 ||
|-
| 300nt V2 kit (eg. 150PE) || $2,233.00 ||
|-
| 500nt V2 kit (eg. 250PE) || $2,464.00 ||
|-
| 600nt V3 kit (eg. 300PE) || $3,080.00 ||
|-
| 500nt '''NANO''' Run (eg. 250PE) || $1,078.00 ||
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 72 hour Flowcell || $1,680.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 72 hour Flowcell || pricing in ilabs || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="5" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| LIGATION BASED NEB UltraII || STD || per sample || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="5" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| HTL NEB UltraII || HTL || 24 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,520.00
|-
| Tagmentation Nextera-XT || STD || per Sample || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $235.00
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,520.00
|-
| 16S Amplicon || HTL || 48 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $910.00
|-
| Other Amplicon || HTL || 48 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $945.00
|-
| colspan="5" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $84.00
|-
| Sample Cleanup || || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="5" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="5" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $4,200.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,940.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $7,000.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,800.00
|-
| || || 96 || $4,480.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $7,000.00
|-
| colspan="5" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $84.00
|-
| Sample Cleanup || || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Standard Ligation || LSK || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $70.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $450.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $225.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $393.00
|-
| Direct RNA || RNA004 || $240.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| DNA || 96 || $980.00 ||
|-
| RNA || 96 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="4" | '''Assisted Service'''
|-
| colspan="4" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $5,460.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="4" | ''scATACseq''
|-
| ATACseq - setup || setup || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="4" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="4" | ''CANCELLATION''
|-
| per session || <24h notice || $280.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Test Array || slide || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! SPATIAL (per slide) !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
|-
| Slide Only || $210.00
|-
| Optimization Only || $1,120.00
|-
| Teton Panel || $10,080.00
|-
| Atlas Short || $7,700.00
|-
| Antibody Add-on || $504.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $21.00
|-
| Fragment Analyzer: Preloaded row || per row (12) || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $42.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $70.00
|-
| qPCR only (Short read quantification test) || per sample || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="4" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $66.00
|-
| FFPE Curl || per curl || $84.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Dr.Oligo || Assisted || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $420.00 ||
|-
| || add Quench || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to Non-MIT users.

== OTHER LAB SERVICES ==

Email biomicro@mit.edu for more information.

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $28.00 || per hour
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $105.00 || per gel (5 lanes)
|-
| Training || $50.00 || per session
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $27.00 || per hour
|-
| colspan="5" | '''LIQUID HANDLING'''
|-
| Tecan EVO 150 || $105.00 || per 1h block - based on scheduled time.
|-
| non-filter tips || $11.20 || per box
|-
| Filter tips || $16.80 || per box
|-
| Training || $91.00 || per hour
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Informatics Support || per hour || $205.00 || Very limited support for non-MIT users
|-
| Ingenuity Pathway Analysis || per dataset || $150.00 || Recovery of licence cost.
|}

BioMicroCenter:Pricing

2026-04-15T17:35:34Z

Cstjohn: /* SHORT READ LIBRARY - RNA */

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,660.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,400.00 || per lane ||
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,710.00 || per flowcell ||
|-
| || One lane (~400M reads) || $1,925.00 || per lane ||
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,900.00 || per lane ||
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $420.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $2,520.00 || per flowcell ||
|-
| 150 PE: F3 Lane (~100M reads) || $630.00 || per lane ||
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
! Length !! Reads !! !! !!
|-
| 300nt || 5M || $1,001.00 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,456.00 ||
|-
| 100nt || 25M || $1,295.00 ||
|-
| 300nt || 25M || $1,867.60 ||
|-
| 600nt || 25M || $2,198.00 ||
|-
| '''1000nt''' || 25M || $3,626.00 ||
|-
| 100nt || 100M || $2,156.00 ||
|-
| 300nt || 100M || $2,975.00 ||
|-
| 600nt || 50M || $2,975.00 ||
|}

==== MiSeq i100 Walkup ====

Walkup service is not available to Non-MIT users.

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,848.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $2,002.00 ||
|-
| 300nt V2 kit (eg. 150PE) || $2,233.00 ||
|-
| 500nt V2 kit (eg. 250PE) || $2,464.00 ||
|-
| 600nt V3 kit (eg. 300PE) || $3,080.00 ||
|-
| 500nt '''NANO''' Run (eg. 250PE) || $1,078.00 ||
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 72 hour Flowcell || $1,680.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 72 hour Flowcell || pricing in ilabs || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="5" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| LIGATION BASED NEB UltraII || STD || per sample || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="5" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. ||
|-
| HTL NEB UltraII || HTL || 24 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,520.00 ||
|-
| Tagmentation Nextera-XT || STD || per Sample || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $235.00 ||
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,520.00 ||
|-
| 16S Amplicon || HTL || 48 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $910.00 ||
|-
| Other Amplicon || HTL || 48 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $945.00 ||
|-
| colspan="5" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $84.00 ||
|-
| Sample Cleanup || || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="5" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="5" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $4,200.00
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,940.00
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $7,000.00
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,800.00
|-
| || || 96 || $4,480.00
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $7,000.00
|-
| colspan="5" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges.
|-
| High Throughput Setup || || 48 samples || $84.00
|-
| Sample Cleanup || || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Standard Ligation || LSK || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $70.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $450.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $225.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $393.00
|-
| Direct RNA || RNA004 || $240.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| DNA || 96 || $980.00 ||
|-
| RNA || 96 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="4" | '''Assisted Service'''
|-
| colspan="4" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $5,460.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="4" | ''scATACseq''
|-
| ATACseq - setup || setup || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="4" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="4" | ''CANCELLATION''
|-
| per session || <24h notice || $280.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Test Array || slide || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! SPATIAL (per slide) !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
|-
| Slide Only || $210.00
|-
| Optimization Only || $1,120.00
|-
| Teton Panel || $10,080.00
|-
| Atlas Short || $7,700.00
|-
| Antibody Add-on || $504.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $21.00
|-
| Fragment Analyzer: Preloaded row || per row (12) || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $42.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $70.00
|-
| qPCR only (Short read quantification test) || per sample || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="4" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $66.00
|-
| FFPE Curl || per curl || $84.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Dr.Oligo || Assisted || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $420.00 ||
|-
| || add Quench || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to Non-MIT users.

== OTHER LAB SERVICES ==

Email biomicro@mit.edu for more information.

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $28.00 || per hour
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $105.00 || per gel (5 lanes)
|-
| Training || $50.00 || per session
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $27.00 || per hour
|-
| colspan="5" | '''LIQUID HANDLING'''
|-
| Tecan EVO 150 || $105.00 || per 1h block - based on scheduled time.
|-
| non-filter tips || $11.20 || per box
|-
| Filter tips || $16.80 || per box
|-
| Training || $91.00 || per hour
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Informatics Support || per hour || $205.00 || Very limited support for non-MIT users
|-
| Ingenuity Pathway Analysis || per dataset || $150.00 || Recovery of licence cost.
|}

BioMicroCenter:Pricing

2026-04-15T17:34:56Z

Cstjohn: /* LONG READ LIBRARIES */

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,660.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,400.00 || per lane ||
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,710.00 || per flowcell ||
|-
| || One lane (~400M reads) || $1,925.00 || per lane ||
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,900.00 || per lane ||
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $420.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $2,520.00 || per flowcell ||
|-
| 150 PE: F3 Lane (~100M reads) || $630.00 || per lane ||
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
! Length !! Reads !! !! !!
|-
| 300nt || 5M || $1,001.00 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,456.00 ||
|-
| 100nt || 25M || $1,295.00 ||
|-
| 300nt || 25M || $1,867.60 ||
|-
| 600nt || 25M || $2,198.00 ||
|-
| '''1000nt''' || 25M || $3,626.00 ||
|-
| 100nt || 100M || $2,156.00 ||
|-
| 300nt || 100M || $2,975.00 ||
|-
| 600nt || 50M || $2,975.00 ||
|}

==== MiSeq i100 Walkup ====

Walkup service is not available to Non-MIT users.

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,848.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $2,002.00 ||
|-
| 300nt V2 kit (eg. 150PE) || $2,233.00 ||
|-
| 500nt V2 kit (eg. 250PE) || $2,464.00 ||
|-
| 600nt V3 kit (eg. 300PE) || $3,080.00 ||
|-
| 500nt '''NANO''' Run (eg. 250PE) || $1,078.00 ||
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 72 hour Flowcell || $1,680.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 72 hour Flowcell || pricing in ilabs || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="5" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| LIGATION BASED NEB UltraII || STD || per sample || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="5" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. ||
|-
| HTL NEB UltraII || HTL || 24 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,520.00 ||
|-
| Tagmentation Nextera-XT || STD || per Sample || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $235.00 ||
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,520.00 ||
|-
| 16S Amplicon || HTL || 48 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $910.00 ||
|-
| Other Amplicon || HTL || 48 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $945.00 ||
|-
| colspan="5" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $84.00 ||
|-
| Sample Cleanup || || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="5" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="5" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. ||
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $4,200.00 ||
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,940.00 ||
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $7,000.00 ||
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,800.00 ||
|-
| || || 96 || $4,480.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $7,000.00 ||
|-
| colspan="5" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $84.00 ||
|-
| Sample Cleanup || || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Standard Ligation || LSK || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $70.00
|-
| SMARTseq -> Ligation || Cv4+LSK || $450.00
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $225.00
|-
| Long Insert Prep || RAD (modified, target >100kb) || $393.00
|-
| Direct RNA || RNA004 || $240.00
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| DNA || 96 || $980.00 ||
|-
| RNA || 96 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="4" | '''Assisted Service'''
|-
| colspan="4" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $5,460.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="4" | ''scATACseq''
|-
| ATACseq - setup || setup || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="4" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="4" | ''CANCELLATION''
|-
| per session || <24h notice || $280.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Test Array || slide || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! SPATIAL (per slide) !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
|-
| Slide Only || $210.00
|-
| Optimization Only || $1,120.00
|-
| Teton Panel || $10,080.00
|-
| Atlas Short || $7,700.00
|-
| Antibody Add-on || $504.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $21.00
|-
| Fragment Analyzer: Preloaded row || per row (12) || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $42.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $70.00
|-
| qPCR only (Short read quantification test) || per sample || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="4" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $66.00
|-
| FFPE Curl || per curl || $84.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Dr.Oligo || Assisted || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $420.00 ||
|-
| || add Quench || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to Non-MIT users.

== OTHER LAB SERVICES ==

Email biomicro@mit.edu for more information.

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $28.00 || per hour
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $105.00 || per gel (5 lanes)
|-
| Training || $50.00 || per session
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $27.00 || per hour
|-
| colspan="5" | '''LIQUID HANDLING'''
|-
| Tecan EVO 150 || $105.00 || per 1h block - based on scheduled time.
|-
| non-filter tips || $11.20 || per box
|-
| Filter tips || $16.80 || per box
|-
| Training || $91.00 || per hour
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Informatics Support || per hour || $205.00 || Very limited support for non-MIT users
|-
| Ingenuity Pathway Analysis || per dataset || $150.00 || Recovery of licence cost.
|}

BioMicroCenter:Pricing

2026-04-15T17:34:41Z

Cstjohn: /* 10X CHROMIUM */

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,660.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,400.00 || per lane ||
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,710.00 || per flowcell ||
|-
| || One lane (~400M reads) || $1,925.00 || per lane ||
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,900.00 || per lane ||
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $420.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $2,520.00 || per flowcell ||
|-
| 150 PE: F3 Lane (~100M reads) || $630.00 || per lane ||
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
! Length !! Reads !! !! !!
|-
| 300nt || 5M || $1,001.00 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,456.00 ||
|-
| 100nt || 25M || $1,295.00 ||
|-
| 300nt || 25M || $1,867.60 ||
|-
| 600nt || 25M || $2,198.00 ||
|-
| '''1000nt''' || 25M || $3,626.00 ||
|-
| 100nt || 100M || $2,156.00 ||
|-
| 300nt || 100M || $2,975.00 ||
|-
| 600nt || 50M || $2,975.00 ||
|}

==== MiSeq i100 Walkup ====

Walkup service is not available to Non-MIT users.

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,848.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $2,002.00 ||
|-
| 300nt V2 kit (eg. 150PE) || $2,233.00 ||
|-
| 500nt V2 kit (eg. 250PE) || $2,464.00 ||
|-
| 600nt V3 kit (eg. 300PE) || $3,080.00 ||
|-
| 500nt '''NANO''' Run (eg. 250PE) || $1,078.00 ||
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 72 hour Flowcell || $1,680.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 72 hour Flowcell || pricing in ilabs || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="5" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| LIGATION BASED NEB UltraII || STD || per sample || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="5" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. ||
|-
| HTL NEB UltraII || HTL || 24 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,520.00 ||
|-
| Tagmentation Nextera-XT || STD || per Sample || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $235.00 ||
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,520.00 ||
|-
| 16S Amplicon || HTL || 48 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $910.00 ||
|-
| Other Amplicon || HTL || 48 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $945.00 ||
|-
| colspan="5" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $84.00 ||
|-
| Sample Cleanup || || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="5" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="5" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. ||
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $4,200.00 ||
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,940.00 ||
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $7,000.00 ||
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,800.00 ||
|-
| || || 96 || $4,480.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $7,000.00 ||
|-
| colspan="5" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $84.00 ||
|-
| Sample Cleanup || || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Standard Ligation || LSK || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $70.00 ||
|-
| SMARTseq -> Ligation || Cv4+LSK || $450.00 ||
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $225.00 ||
|-
| Long Insert Prep || RAD (modified, target >100kb) || $393.00 ||
|-
| Direct RNA || RNA004 || $240.00 ||
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| DNA || 96 || $980.00 ||
|-
| RNA || 96 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="4" | '''Assisted Service'''
|-
| colspan="4" | ''DIGITAL GENE EXPRESSION''
|-
| 5' RNA / 3' RNA - setup || setup || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $5,460.00
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="4" | ''scATACseq''
|-
| ATACseq - setup || setup || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="4" | ''Multiome (ATAC+3'RNA)''
|-
| MULTI - setup || setup || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="4" | ''CANCELLATION''
|-
| per session || <24h notice || $280.00
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Test Array || slide || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! SPATIAL (per slide) !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
|-
| Slide Only || $210.00
|-
| Optimization Only || $1,120.00
|-
| Teton Panel || $10,080.00
|-
| Atlas Short || $7,700.00
|-
| Antibody Add-on || $504.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $21.00
|-
| Fragment Analyzer: Preloaded row || per row (12) || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $42.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $70.00
|-
| qPCR only (Short read quantification test) || per sample || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="4" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $66.00
|-
| FFPE Curl || per curl || $84.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Dr.Oligo || Assisted || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $420.00 ||
|-
| || add Quench || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to Non-MIT users.

== OTHER LAB SERVICES ==

Email biomicro@mit.edu for more information.

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $28.00 || per hour
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $105.00 || per gel (5 lanes)
|-
| Training || $50.00 || per session
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $27.00 || per hour
|-
| colspan="5" | '''LIQUID HANDLING'''
|-
| Tecan EVO 150 || $105.00 || per 1h block - based on scheduled time.
|-
| non-filter tips || $11.20 || per box
|-
| Filter tips || $16.80 || per box
|-
| Training || $91.00 || per hour
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Informatics Support || per hour || $205.00 || Very limited support for non-MIT users
|-
| Ingenuity Pathway Analysis || per dataset || $150.00 || Recovery of licence cost.
|}

BioMicroCenter:Pricing

2026-04-15T17:33:51Z

Cstjohn: /* AVITI24 */

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,660.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,400.00 || per lane ||
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,710.00 || per flowcell ||
|-
| || One lane (~400M reads) || $1,925.00 || per lane ||
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,900.00 || per lane ||
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $420.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $2,520.00 || per flowcell ||
|-
| 150 PE: F3 Lane (~100M reads) || $630.00 || per lane ||
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
! Length !! Reads !! !! !!
|-
| 300nt || 5M || $1,001.00 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,456.00 ||
|-
| 100nt || 25M || $1,295.00 ||
|-
| 300nt || 25M || $1,867.60 ||
|-
| 600nt || 25M || $2,198.00 ||
|-
| '''1000nt''' || 25M || $3,626.00 ||
|-
| 100nt || 100M || $2,156.00 ||
|-
| 300nt || 100M || $2,975.00 ||
|-
| 600nt || 50M || $2,975.00 ||
|}

==== MiSeq i100 Walkup ====

Walkup service is not available to Non-MIT users.

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,848.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $2,002.00 ||
|-
| 300nt V2 kit (eg. 150PE) || $2,233.00 ||
|-
| 500nt V2 kit (eg. 250PE) || $2,464.00 ||
|-
| 600nt V3 kit (eg. 300PE) || $3,080.00 ||
|-
| 500nt '''NANO''' Run (eg. 250PE) || $1,078.00 ||
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 72 hour Flowcell || $1,680.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 72 hour Flowcell || pricing in ilabs || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="5" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| LIGATION BASED NEB UltraII || STD || per sample || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="5" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. ||
|-
| HTL NEB UltraII || HTL || 24 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,520.00 ||
|-
| Tagmentation Nextera-XT || STD || per Sample || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $235.00 ||
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,520.00 ||
|-
| 16S Amplicon || HTL || 48 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $910.00 ||
|-
| Other Amplicon || HTL || 48 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $945.00 ||
|-
| colspan="5" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $84.00 ||
|-
| Sample Cleanup || || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="5" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="5" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. ||
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $4,200.00 ||
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,940.00 ||
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $7,000.00 ||
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,800.00 ||
|-
| || || 96 || $4,480.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $7,000.00 ||
|-
| colspan="5" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $84.00 ||
|-
| Sample Cleanup || || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Standard Ligation || LSK || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $70.00 ||
|-
| SMARTseq -> Ligation || Cv4+LSK || $450.00 ||
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $225.00 ||
|-
| Long Insert Prep || RAD (modified, target >100kb) || $393.00 ||
|-
| Direct RNA || RNA004 || $240.00 ||
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| DNA || 96 || $980.00 ||
|-
| RNA || 96 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="4" | '''Assisted Service''' ||
|-
| colspan="4" | ''DIGITAL GENE EXPRESSION'' ||
|-
| 5' RNA / 3' RNA - setup || setup || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $5,460.00 ||
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="4" | ''scATACseq'' ||
|-
| ATACseq - setup || setup || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="4" | ''Multiome (ATAC+3'RNA)'' ||
|-
| MULTI - setup || setup || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="4" | ''CANCELLATION'' ||
|-
| per session || <24h notice || $280.00 ||
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Test Array || slide || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! SPATIAL (per slide) !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
|-
| Slide Only || $210.00
|-
| Optimization Only || $1,120.00
|-
| Teton Panel || $10,080.00
|-
| Atlas Short || $7,700.00
|-
| Antibody Add-on || $504.00
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $21.00
|-
| Fragment Analyzer: Preloaded row || per row (12) || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $42.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $70.00
|-
| qPCR only (Short read quantification test) || per sample || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="4" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $66.00
|-
| FFPE Curl || per curl || $84.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Dr.Oligo || Assisted || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $420.00 ||
|-
| || add Quench || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to Non-MIT users.

== OTHER LAB SERVICES ==

Email biomicro@mit.edu for more information.

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $28.00 || per hour
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $105.00 || per gel (5 lanes)
|-
| Training || $50.00 || per session
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $27.00 || per hour
|-
| colspan="5" | '''LIQUID HANDLING'''
|-
| Tecan EVO 150 || $105.00 || per 1h block - based on scheduled time.
|-
| non-filter tips || $11.20 || per box
|-
| Filter tips || $16.80 || per box
|-
| Training || $91.00 || per hour
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Informatics Support || per hour || $205.00 || Very limited support for non-MIT users
|-
| Ingenuity Pathway Analysis || per dataset || $150.00 || Recovery of licence cost.
|}

BioMicroCenter:Pricing

2026-04-15T17:33:31Z

Cstjohn: /* QUALITY CONTROL */

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,660.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,400.00 || per lane ||
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,710.00 || per flowcell ||
|-
| || One lane (~400M reads) || $1,925.00 || per lane ||
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,900.00 || per lane ||
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $420.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $2,520.00 || per flowcell ||
|-
| 150 PE: F3 Lane (~100M reads) || $630.00 || per lane ||
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
! Length !! Reads !! !! !!
|-
| 300nt || 5M || $1,001.00 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,456.00 ||
|-
| 100nt || 25M || $1,295.00 ||
|-
| 300nt || 25M || $1,867.60 ||
|-
| 600nt || 25M || $2,198.00 ||
|-
| '''1000nt''' || 25M || $3,626.00 ||
|-
| 100nt || 100M || $2,156.00 ||
|-
| 300nt || 100M || $2,975.00 ||
|-
| 600nt || 50M || $2,975.00 ||
|}

==== MiSeq i100 Walkup ====

Walkup service is not available to Non-MIT users.

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,848.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $2,002.00 ||
|-
| 300nt V2 kit (eg. 150PE) || $2,233.00 ||
|-
| 500nt V2 kit (eg. 250PE) || $2,464.00 ||
|-
| 600nt V3 kit (eg. 300PE) || $3,080.00 ||
|-
| 500nt '''NANO''' Run (eg. 250PE) || $1,078.00 ||
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 72 hour Flowcell || $1,680.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 72 hour Flowcell || pricing in ilabs || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="5" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| LIGATION BASED NEB UltraII || STD || per sample || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="5" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. ||
|-
| HTL NEB UltraII || HTL || 24 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,520.00 ||
|-
| Tagmentation Nextera-XT || STD || per Sample || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $235.00 ||
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,520.00 ||
|-
| 16S Amplicon || HTL || 48 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $910.00 ||
|-
| Other Amplicon || HTL || 48 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $945.00 ||
|-
| colspan="5" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $84.00 ||
|-
| Sample Cleanup || || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="5" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="5" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. ||
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $4,200.00 ||
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,940.00 ||
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $7,000.00 ||
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,800.00 ||
|-
| || || 96 || $4,480.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $7,000.00 ||
|-
| colspan="5" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $84.00 ||
|-
| Sample Cleanup || || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Standard Ligation || LSK || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $70.00 ||
|-
| SMARTseq -> Ligation || Cv4+LSK || $450.00 ||
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $225.00 ||
|-
| Long Insert Prep || RAD (modified, target >100kb) || $393.00 ||
|-
| Direct RNA || RNA004 || $240.00 ||
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| DNA || 96 || $980.00 ||
|-
| RNA || 96 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="4" | '''Assisted Service''' ||
|-
| colspan="4" | ''DIGITAL GENE EXPRESSION'' ||
|-
| 5' RNA / 3' RNA - setup || setup || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $5,460.00 ||
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="4" | ''scATACseq'' ||
|-
| ATACseq - setup || setup || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="4" | ''Multiome (ATAC+3'RNA)'' ||
|-
| MULTI - setup || setup || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="4" | ''CANCELLATION'' ||
|-
| per session || <24h notice || $280.00 ||
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Test Array || slide || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! SPATIAL (per slide) !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
|-
| Slide Only || $210.00 ||
|-
| Optimization Only || $1,120.00 ||
|-
| Teton Panel || $10,080.00 ||
|-
| Atlas Short || $7,700.00 ||
|-
| Antibody Add-on || $504.00 ||
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $21.00
|-
| Fragment Analyzer: Preloaded row || per row (12) || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $42.00
|-
| FemtoPulse: Preloaded Row || per row (12) || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $70.00
|-
| qPCR only (Short read quantification test) || per sample || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="4" | '''Sample QC services:'''
|-
| Fresh Frozen Curl || per curl || $66.00
|-
| FFPE Curl || per curl || $84.00
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Dr.Oligo || Assisted || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $420.00 ||
|-
| || add Quench || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to Non-MIT users.

== OTHER LAB SERVICES ==

Email biomicro@mit.edu for more information.

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $28.00 || per hour
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $105.00 || per gel (5 lanes)
|-
| Training || $50.00 || per session
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $27.00 || per hour
|-
| colspan="5" | '''LIQUID HANDLING'''
|-
| Tecan EVO 150 || $105.00 || per 1h block - based on scheduled time.
|-
| non-filter tips || $11.20 || per box
|-
| Filter tips || $16.80 || per box
|-
| Training || $91.00 || per hour
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Informatics Support || per hour || $205.00 || Very limited support for non-MIT users
|-
| Ingenuity Pathway Analysis || per dataset || $150.00 || Recovery of licence cost.
|}

BioMicroCenter:Pricing

2026-04-15T17:32:47Z

Cstjohn: /* OTHER LAB SERVICES */

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,660.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,400.00 || per lane ||
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,710.00 || per flowcell ||
|-
| || One lane (~400M reads) || $1,925.00 || per lane ||
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,900.00 || per lane ||
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $420.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $2,520.00 || per flowcell ||
|-
| 150 PE: F3 Lane (~100M reads) || $630.00 || per lane ||
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
! Length !! Reads !! !! !!
|-
| 300nt || 5M || $1,001.00 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,456.00 ||
|-
| 100nt || 25M || $1,295.00 ||
|-
| 300nt || 25M || $1,867.60 ||
|-
| 600nt || 25M || $2,198.00 ||
|-
| '''1000nt''' || 25M || $3,626.00 ||
|-
| 100nt || 100M || $2,156.00 ||
|-
| 300nt || 100M || $2,975.00 ||
|-
| 600nt || 50M || $2,975.00 ||
|}

==== MiSeq i100 Walkup ====

Walkup service is not available to Non-MIT users.

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,848.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $2,002.00 ||
|-
| 300nt V2 kit (eg. 150PE) || $2,233.00 ||
|-
| 500nt V2 kit (eg. 250PE) || $2,464.00 ||
|-
| 600nt V3 kit (eg. 300PE) || $3,080.00 ||
|-
| 500nt '''NANO''' Run (eg. 250PE) || $1,078.00 ||
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 72 hour Flowcell || $1,680.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 72 hour Flowcell || pricing in ilabs || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="5" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| LIGATION BASED NEB UltraII || STD || per sample || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="5" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. ||
|-
| HTL NEB UltraII || HTL || 24 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,520.00 ||
|-
| Tagmentation Nextera-XT || STD || per Sample || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $235.00 ||
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,520.00 ||
|-
| 16S Amplicon || HTL || 48 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $910.00 ||
|-
| Other Amplicon || HTL || 48 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $945.00 ||
|-
| colspan="5" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $84.00 ||
|-
| Sample Cleanup || || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="5" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="5" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. ||
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $4,200.00 ||
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,940.00 ||
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $7,000.00 ||
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,800.00 ||
|-
| || || 96 || $4,480.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $7,000.00 ||
|-
| colspan="5" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $84.00 ||
|-
| Sample Cleanup || || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Standard Ligation || LSK || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $70.00 ||
|-
| SMARTseq -> Ligation || Cv4+LSK || $450.00 ||
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $225.00 ||
|-
| Long Insert Prep || RAD (modified, target >100kb) || $393.00 ||
|-
| Direct RNA || RNA004 || $240.00 ||
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| DNA || 96 || $980.00 ||
|-
| RNA || 96 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="4" | '''Assisted Service''' ||
|-
| colspan="4" | ''DIGITAL GENE EXPRESSION'' ||
|-
| 5' RNA / 3' RNA - setup || setup || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $5,460.00 ||
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="4" | ''scATACseq'' ||
|-
| ATACseq - setup || setup || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="4" | ''Multiome (ATAC+3'RNA)'' ||
|-
| MULTI - setup || setup || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="4" | ''CANCELLATION'' ||
|-
| per session || <24h notice || $280.00 ||
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Test Array || slide || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! SPATIAL (per slide) !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
|-
| Slide Only || $210.00 ||
|-
| Optimization Only || $1,120.00 ||
|-
| Teton Panel || $10,080.00 ||
|-
| Atlas Short || $7,700.00 ||
|-
| Antibody Add-on || $504.00 ||
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $21.00 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $42.00 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $70.00 ||
|-
| qPCR only (Short read quantification test) || per sample || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="4" | '''Sample QC services:''' ||
|-
| Fresh Frozen Curl || per curl || $66.00 ||
|-
| FFPE Curl || per curl || $84.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Dr.Oligo || Assisted || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $420.00 ||
|-
| || add Quench || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to Non-MIT users.

== OTHER LAB SERVICES ==

Email biomicro@mit.edu for more information.

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $28.00 || per hour
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $105.00 || per gel (5 lanes)
|-
| Training || $50.00 || per session
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $27.00 || per hour
|-
| colspan="5" | '''LIQUID HANDLING'''
|-
| Tecan EVO 150 || $105.00 || per 1h block - based on scheduled time.
|-
| non-filter tips || $11.20 || per box
|-
| Filter tips || $16.80 || per box
|-
| Training || $91.00 || per hour
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Informatics Support || per hour || $205.00 || Very limited support for non-MIT users
|-
| Ingenuity Pathway Analysis || per dataset || $150.00 || Recovery of licence cost.
|}

BioMicroCenter:Pricing

2026-04-15T17:31:03Z

Cstjohn: /* OTHER LAB SERVICES */

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,660.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,400.00 || per lane ||
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,710.00 || per flowcell ||
|-
| || One lane (~400M reads) || $1,925.00 || per lane ||
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,900.00 || per lane ||
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $420.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $2,520.00 || per flowcell ||
|-
| 150 PE: F3 Lane (~100M reads) || $630.00 || per lane ||
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
! Length !! Reads !! !! !!
|-
| 300nt || 5M || $1,001.00 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,456.00 ||
|-
| 100nt || 25M || $1,295.00 ||
|-
| 300nt || 25M || $1,867.60 ||
|-
| 600nt || 25M || $2,198.00 ||
|-
| '''1000nt''' || 25M || $3,626.00 ||
|-
| 100nt || 100M || $2,156.00 ||
|-
| 300nt || 100M || $2,975.00 ||
|-
| 600nt || 50M || $2,975.00 ||
|}

==== MiSeq i100 Walkup ====

Walkup service is not available to Non-MIT users.

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,848.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $2,002.00 ||
|-
| 300nt V2 kit (eg. 150PE) || $2,233.00 ||
|-
| 500nt V2 kit (eg. 250PE) || $2,464.00 ||
|-
| 600nt V3 kit (eg. 300PE) || $3,080.00 ||
|-
| 500nt '''NANO''' Run (eg. 250PE) || $1,078.00 ||
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 72 hour Flowcell || $1,680.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 72 hour Flowcell || pricing in ilabs || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="5" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| LIGATION BASED NEB UltraII || STD || per sample || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="5" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. ||
|-
| HTL NEB UltraII || HTL || 24 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,520.00 ||
|-
| Tagmentation Nextera-XT || STD || per Sample || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $235.00 ||
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,520.00 ||
|-
| 16S Amplicon || HTL || 48 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $910.00 ||
|-
| Other Amplicon || HTL || 48 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $945.00 ||
|-
| colspan="5" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $84.00 ||
|-
| Sample Cleanup || || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="5" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="5" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. ||
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $4,200.00 ||
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,940.00 ||
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $7,000.00 ||
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,800.00 ||
|-
| || || 96 || $4,480.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $7,000.00 ||
|-
| colspan="5" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $84.00 ||
|-
| Sample Cleanup || || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Standard Ligation || LSK || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $70.00 ||
|-
| SMARTseq -> Ligation || Cv4+LSK || $450.00 ||
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $225.00 ||
|-
| Long Insert Prep || RAD (modified, target >100kb) || $393.00 ||
|-
| Direct RNA || RNA004 || $240.00 ||
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| DNA || 96 || $980.00 ||
|-
| RNA || 96 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="4" | '''Assisted Service''' ||
|-
| colspan="4" | ''DIGITAL GENE EXPRESSION'' ||
|-
| 5' RNA / 3' RNA - setup || setup || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $5,460.00 ||
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="4" | ''scATACseq'' ||
|-
| ATACseq - setup || setup || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="4" | ''Multiome (ATAC+3'RNA)'' ||
|-
| MULTI - setup || setup || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="4" | ''CANCELLATION'' ||
|-
| per session || <24h notice || $280.00 ||
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Test Array || slide || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! SPATIAL (per slide) !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
|-
| Slide Only || $210.00 ||
|-
| Optimization Only || $1,120.00 ||
|-
| Teton Panel || $10,080.00 ||
|-
| Atlas Short || $7,700.00 ||
|-
| Antibody Add-on || $504.00 ||
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $21.00 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $42.00 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $70.00 ||
|-
| qPCR only (Short read quantification test) || per sample || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="4" | '''Sample QC services:''' ||
|-
| Fresh Frozen Curl || per curl || $66.00 ||
|-
| FFPE Curl || per curl || $84.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Dr.Oligo || Assisted || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $420.00 ||
|-
| || add Quench || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to Non-MIT users.

== OTHER LAB SERVICES ==

Email biomicro@mit.edu for more information.

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit
|-
| colspan="5" | '''COVARIS SONICATOR'''
|-
| COVARIS E220 || $28.00 || per hour
|-
| colspan="5" | '''PIPPIN PREP'''
|-
| Gel Isolation || $105.00 || per gel (5 lanes)
|-
| Training || $50.00 || per session
|-
| colspan="5" | '''PLATE READER'''
|-
| Varioskan || $27.00 || per hour
|-
| colspan="5" | '''ROBOTICS'''
|-
| Tecan EVO 150 || $105.00 || per 1h block - based on scheduled time.
|-
| non-filter tips || $11.20 || per box
|-
| Filter tips || $16.80 || per box
|-
| Training || $91.00 || per hour
|-
| colspan="5" | '''NANODROP'''
|-
| Nanodrop || -- || no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Informatics Support || per hour || $205.00 || Very limited support for non-MIT users
|-
| Ingenuity Pathway Analysis || per dataset || $150.00 || Recovery of licence cost.
|}

BioMicroCenter:Pricing

2026-04-15T17:30:21Z

Cstjohn: /* OTHER LAB SERVICES */

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,660.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,400.00 || per lane ||
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,710.00 || per flowcell ||
|-
| || One lane (~400M reads) || $1,925.00 || per lane ||
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,900.00 || per lane ||
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $420.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $2,520.00 || per flowcell ||
|-
| 150 PE: F3 Lane (~100M reads) || $630.00 || per lane ||
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
! Length !! Reads !! !! !!
|-
| 300nt || 5M || $1,001.00 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,456.00 ||
|-
| 100nt || 25M || $1,295.00 ||
|-
| 300nt || 25M || $1,867.60 ||
|-
| 600nt || 25M || $2,198.00 ||
|-
| '''1000nt''' || 25M || $3,626.00 ||
|-
| 100nt || 100M || $2,156.00 ||
|-
| 300nt || 100M || $2,975.00 ||
|-
| 600nt || 50M || $2,975.00 ||
|}

==== MiSeq i100 Walkup ====

Walkup service is not available to Non-MIT users.

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,848.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $2,002.00 ||
|-
| 300nt V2 kit (eg. 150PE) || $2,233.00 ||
|-
| 500nt V2 kit (eg. 250PE) || $2,464.00 ||
|-
| 600nt V3 kit (eg. 300PE) || $3,080.00 ||
|-
| 500nt '''NANO''' Run (eg. 250PE) || $1,078.00 ||
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 72 hour Flowcell || $1,680.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 72 hour Flowcell || pricing in ilabs || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="5" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| LIGATION BASED NEB UltraII || STD || per sample || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="5" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. ||
|-
| HTL NEB UltraII || HTL || 24 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,520.00 ||
|-
| Tagmentation Nextera-XT || STD || per Sample || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $235.00 ||
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,520.00 ||
|-
| 16S Amplicon || HTL || 48 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $910.00 ||
|-
| Other Amplicon || HTL || 48 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $945.00 ||
|-
| colspan="5" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $84.00 ||
|-
| Sample Cleanup || || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="5" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="5" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. ||
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $4,200.00 ||
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,940.00 ||
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $7,000.00 ||
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,800.00 ||
|-
| || || 96 || $4,480.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $7,000.00 ||
|-
| colspan="5" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $84.00 ||
|-
| Sample Cleanup || || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Standard Ligation || LSK || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $70.00 ||
|-
| SMARTseq -> Ligation || Cv4+LSK || $450.00 ||
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $225.00 ||
|-
| Long Insert Prep || RAD (modified, target >100kb) || $393.00 ||
|-
| Direct RNA || RNA004 || $240.00 ||
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| DNA || 96 || $980.00 ||
|-
| RNA || 96 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="4" | '''Assisted Service''' ||
|-
| colspan="4" | ''DIGITAL GENE EXPRESSION'' ||
|-
| 5' RNA / 3' RNA - setup || setup || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $5,460.00 ||
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="4" | ''scATACseq'' ||
|-
| ATACseq - setup || setup || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="4" | ''Multiome (ATAC+3'RNA)'' ||
|-
| MULTI - setup || setup || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="4" | ''CANCELLATION'' ||
|-
| per session || <24h notice || $280.00 ||
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Test Array || slide || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! SPATIAL (per slide) !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
|-
| Slide Only || $210.00 ||
|-
| Optimization Only || $1,120.00 ||
|-
| Teton Panel || $10,080.00 ||
|-
| Atlas Short || $7,700.00 ||
|-
| Antibody Add-on || $504.00 ||
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $21.00 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $42.00 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $70.00 ||
|-
| qPCR only (Short read quantification test) || per sample || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="4" | '''Sample QC services:''' ||
|-
| Fresh Frozen Curl || per curl || $66.00 ||
|-
| FFPE Curl || per curl || $84.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Dr.Oligo || Assisted || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $420.00 ||
|-
| || add Quench || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to Non-MIT users.

== OTHER LAB SERVICES ==

Email biomicro@mit.edu for more information.

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !!
|-
| colspan="5" | '''COVARIS SONICATOR''' ||
|-
| COVARIS E220 || $28.00 || per hour ||
|-
| colspan="5" | '''PIPPIN PREP''' ||
|-
| Gel Isolation || $105.00 || per gel (5 lanes) ||
|-
| Training || $50.00 || per session ||
|-
| colspan="5" | '''PLATE READER''' ||
|-
| Varioskan || $27.00 || per hour ||
|-
| colspan="5" | '''ROBOTICS''' ||
|-
| Tecan EVO 150 || $105.00 || per 1h block - based on scheduled time. ||
|-
| non-filter tips || $11.20 || per box ||
|-
| Filter tips || $16.80 || per box ||
|-
| Training || $91.00 || per hour ||
|-
| colspan="5" | '''NANODROP''' ||
|-
| Nanodrop || -- || no charge ||
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Informatics Support || per hour || $205.00 || Very limited support for non-MIT users
|-
| Ingenuity Pathway Analysis || per dataset || $150.00 || Recovery of licence cost.
|}

BioMicroCenter:Pricing

2026-04-15T17:27:14Z

Cstjohn: /* COMPUTATIONAL SERVICES */

== SEQUENCING - MID OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===

{| class="wikitable"
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,660.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| || One lane (~400M reads) || $1,400.00 || per lane ||
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,710.00 || per flowcell ||
|-
| || One lane (~400M reads) || $1,925.00 || per lane ||
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,900.00 || per lane ||
|}

== SEQUENCING - LOW OUTPUT ==

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===

{| class="wikitable"
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 50 PE: F3 Lane (~100M reads) || $420.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
| Rapid 300nt: F3 flowcell* || $2,520.00 || per flowcell ||
|-
| 150 PE: F3 Lane (~100M reads) || $630.00 || per lane ||
|}

<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>

=== MISEQ SEQUENCING ===

==== MiSeq i100 Assisted ====

{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
! Length !! Reads !! !! !!
|-
| 300nt || 5M || $1,001.00 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,456.00 ||
|-
| 100nt || 25M || $1,295.00 ||
|-
| 300nt || 25M || $1,867.60 ||
|-
| 600nt || 25M || $2,198.00 ||
|-
| '''1000nt''' || 25M || $3,626.00 ||
|-
| 100nt || 100M || $2,156.00 ||
|-
| 300nt || 100M || $2,975.00 ||
|-
| 600nt || 50M || $2,975.00 ||
|}

==== MiSeq i100 Walkup ====

Walkup service is not available to Non-MIT users.

==== MiSeq classic ====

* MiSeq is off contract and will be retired upon instrument failure

{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,848.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $2,002.00 ||
|-
| 300nt V2 kit (eg. 150PE) || $2,233.00 ||
|-
| 500nt V2 kit (eg. 250PE) || $2,464.00 ||
|-
| 600nt V3 kit (eg. 300PE) || $3,080.00 ||
|-
| 500nt '''NANO''' Run (eg. 250PE) || $1,078.00 ||
|}

=== OTHER SHORT READ SERVICES ===

{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $560.00 || per plate || excludes column 1 (used for controls)
|}

== LONG READ SEQUENCING ==

{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 72 hour Flowcell || $1,680.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 72 hour Flowcell || pricing in ilabs || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}

<pre>
^^ - through collaboration with nearby academic shared resources
</pre>

== LIBRARY GENERATION ==

=== SHORT READ LIBRARY - CONVERSION ===

{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}

=== SHORT READ LIBRARY - DNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="5" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| LIGATION BASED NEB UltraII || STD || per sample || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="5" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. ||
|-
| HTL NEB UltraII || HTL || 24 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,520.00 ||
|-
| Tagmentation Nextera-XT || STD || per Sample || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $235.00 ||
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
| || HTL || 96 || $2,520.00 ||
|-
| 16S Amplicon || HTL || 48 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
| || HTL || add'l 48 || $910.00 ||
|-
| Other Amplicon || HTL || 48 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
| || HTL || add'l 48 || $945.00 ||
|-
| colspan="5" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $84.00 ||
|-
| Sample Cleanup || || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
|}

=== SHORT READ LIBRARY - RNA ===

{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="5" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="5" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. ||
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || '''HTL''' (NEB only) || 96 || $4,200.00 ||
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
| || '''HTL''' || 96 || $2,940.00 ||
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $7,000.00 ||
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,800.00 ||
|-
| || || 96 || $4,480.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
| || || 96 || $7,000.00 ||
|-
| colspan="5" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup || || 48 samples || $84.00 ||
|-
| Sample Cleanup || || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification || || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
|}

.

=== LONG READ LIBRARIES ===

{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Standard Ligation || LSK || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $70.00 ||
|-
| SMARTseq -> Ligation || Cv4+LSK || $450.00 ||
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $225.00 ||
|-
| Long Insert Prep || RAD (modified, target >100kb) || $393.00 ||
|-
| Direct RNA || RNA004 || $240.00 ||
|}

== SAMPLE EXTRACTION ==

=== CHEMAGIC360 ===

{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| DNA || 96 || $980.00 ||
|-
| RNA || 96 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,400.00 ||
|}

== SINGLE CELL & SPATIAL SERVICES ==

=== 10X CHROMIUM ===

{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="4" | '''Assisted Service''' ||
|-
| colspan="4" | ''DIGITAL GENE EXPRESSION'' ||
|-
| 5' RNA / 3' RNA - setup || setup || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $5,460.00 ||
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="4" | ''scATACseq'' ||
|-
| ATACseq - setup || setup || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="4" | ''Multiome (ATAC+3'RNA)'' ||
|-
| MULTI - setup || setup || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="4" | ''CANCELLATION'' ||
|-
| per session || <24h notice || $280.00 ||
|}

* please let BMC know if you plan to run this method early so we will have reagents ready for you.

=== VISIUM ===

{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Test Array || slide || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $66.00 || RNA isolation from FF scroll
|}

=== AVITI24 ===

{| class="wikitable"
|-
! SPATIAL (per slide) !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
|-
| Slide Only || $210.00 ||
|-
| Optimization Only || $1,120.00 ||
|-
| Teton Panel || $10,080.00 ||
|-
| Atlas Short || $7,700.00 ||
|-
| Antibody Add-on || $504.00 ||
|}

== OTHER GENOMICS SERVICES ==

=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===

{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $21.00 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $42.00 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $70.00 ||
|-
| qPCR only (Short read quantification test) || per sample || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="4" | '''Sample QC services:''' ||
|-
| Fresh Frozen Curl || per curl || $66.00 ||
|-
| FFPE Curl || per curl || $84.00 ||
|}

=== OLIGO SYNTHESIS ===

{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Dr.Oligo || Assisted || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
| || Walkup || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
| || Training || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,400.00 || Per run cost
|-
| || 2000 nucleotide synthesis || $700.00 || Up to 96 samples
|-
| || 8000 nucleotide synthesis || $2,100.00 || Up to 96 samples
|-
| || add Biotin (max 48) || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
| || add Fluor || $420.00 ||
|-
| || add Quench || $140.00 ||
|}

== REAL TIME PCR ==

Real-time PCR services are not available to Non-MIT users.

== OTHER LAB SERVICES ==

{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR''' ||
|-
| COVARIS E220 || $28.00 || per hour || TBA || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP''' ||
|-
| Gel Isolation || $105.00 || per gel (5 lanes) || TBA ||
|-
| Training || $50.00 || per session || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''PLATE READER''' ||
|-
| Varioskan || $27.00 || per hour || TBA || Email biomicro@mit.edu for training.
|-
| colspan="5" | '''ROBOTICS''' ||
|-
| Tecan EVO 150 || $105.00 || per 1h block - based on scheduled time. || TBA||
|-
| non-filter tips || $11.20 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $16.80 || per box || ||
|-
| Training || $91.00 || per hour || Email biomicro@mit.edu for training. ||
|-
| colspan="5" | '''NANODROP''' ||
|-
| Nanodrop || -- || || Walk up service || * no charge
|}

== COMPUTATIONAL SERVICES ==

{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Informatics Support || per hour || $205.00 || Very limited support for non-MIT users
|-
| Ingenuity Pathway Analysis || per dataset || $150.00 || Recovery of licence cost.
|}