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	<id>http://bmcwiki.mit.edu/api.php?action=feedcontributions&amp;feedformat=atom&amp;user=Asoberan</id>
	<title>BioMicro Center - User contributions [en]</title>
	<link rel="self" type="application/atom+xml" href="http://bmcwiki.mit.edu/api.php?action=feedcontributions&amp;feedformat=atom&amp;user=Asoberan"/>
	<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php/Special:Contributions/Asoberan"/>
	<updated>2026-06-17T20:08:09Z</updated>
	<subtitle>User contributions</subtitle>
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	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=BMC_Training&amp;diff=131564</id>
		<title>BMC Training</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=BMC_Training&amp;diff=131564"/>
		<updated>2026-06-01T17:59:19Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: Created page with &amp;quot;STUB&amp;quot;&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;STUB&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=BioMicroCenter&amp;diff=131563</id>
		<title>BioMicroCenter</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=BioMicroCenter&amp;diff=131563"/>
		<updated>2026-06-01T17:50:05Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;&amp;lt;h2 align=&amp;quot;center&amp;quot;&amp;gt; Welcome to the MIT BioMicro Center! &amp;lt;/h2&amp;gt;&lt;br /&gt;
&lt;br /&gt;
{| width=&amp;quot;100%&amp;quot; &lt;br /&gt;
 |- &lt;br /&gt;
 |valign=&amp;quot;top&amp;quot;|&lt;br /&gt;
[[File:logo.png|left]]&lt;br /&gt;
 | valign=&amp;quot;top&amp;quot; align=&amp;quot;left&amp;quot;|&lt;br /&gt;
* [[BioMicroCenter:News|&#039;&#039;&#039;News &amp;amp; General Information&#039;&#039;&#039;]]&lt;br /&gt;
* [[BioMicroCenter:Pricing|Pricing]] / [[MIT:Pricing|MIT Pricing]]&lt;br /&gt;
* [[BioMicroCenter:Submission|Sample Submission]]&lt;br /&gt;
* [[BioMicroCenter:Consumables|Consumables]]&lt;br /&gt;
* [[BioMicroCenter:Consulting|Experimental Design]]&lt;br /&gt;
 | valign=&amp;quot;top&amp;quot; align=&amp;quot;left&amp;quot;|&lt;br /&gt;
* [[BioMicroCenter:AboutBMC|About the BioMicro Center]]&lt;br /&gt;
* [[BioMicroCenter:People| Staff]] &lt;br /&gt;
* [[BioMicroCenter:Consulting| Getting Started]]&lt;br /&gt;
&amp;lt;!-- [[BioMicroCenter:Jobs|Positions Available]] --&amp;gt;&lt;br /&gt;
* [[BioMicroCenter:FAQ|FAQs]]&lt;br /&gt;
|}&lt;br /&gt;
The &#039;&#039;&#039;MIT BioMicro Center&#039;&#039;&#039; is an integrated genomics core facility that provides both expertise and equipment for systems biology. The core has significant resources in sample preparation, next generation sequencing and in high throughput screening as well as bioinformatics and BioIT. The Center is a joint endeavor between the [http://biology.mit.edu Department of Biology], the [http://ki.mit.edu Koch Institute for Integrative Cancer Research] (Integrated Genome Technologies Core (IGT) and Data Science &amp;amp; Multimodal Integration Core (DSMI)), and the [http://web.mit.edu/be Department of Biological Engineering].&lt;br /&gt;
&lt;br /&gt;
{| width=&amp;quot;100%&amp;quot;&lt;br /&gt;
 |- valign=&amp;quot;top&amp;quot;&lt;br /&gt;
 |colspan=2 align=&amp;quot;center&amp;quot; style=&amp;quot;background-color:yellow; border:1px solid purple&amp;quot;| &lt;br /&gt;
 {|&lt;br /&gt;
  |&amp;lt;BIG&amp;gt;&amp;lt;B&amp;gt;[[BioMicroCenter:Assisted_Services|Assisted Services]] &amp;lt;/B&amp;gt;&amp;lt;/BIG&amp;gt;&lt;br /&gt;
 |}&lt;br /&gt;
 |align=&amp;quot;center&amp;quot; style=&amp;quot;background-color:aqua; border:1px solid black&amp;quot;|&lt;br /&gt;
 {|&lt;br /&gt;
  |&amp;lt;BIG&amp;gt;&amp;lt;B&amp;gt; Walkup Services &amp;lt;/B&amp;gt;&amp;lt;/BIG&amp;gt;&lt;br /&gt;
 |}&lt;br /&gt;
&lt;br /&gt;
 |-&lt;br /&gt;
 |valign=&amp;quot;top&amp;quot; align=&amp;quot;center&amp;quot; style=&amp;quot;background-color:transparent; border:1px solid black&amp;quot;| &lt;br /&gt;
 {|&lt;br /&gt;
  |width=&amp;quot;10%&amp;quot; align=&amp;quot;left&amp;quot;|&amp;lt;BIG&amp;gt;&amp;lt;B&amp;gt;Sample Isolation&amp;lt;/B&amp;gt;&amp;lt;/BIG&amp;gt;&lt;br /&gt;
* Sonication ([[BioMicroCenter:Covaris|Covaris E220/R230]])&lt;br /&gt;
* DNA/RNA purification ([[BioMicroCenter:Tecan_Freedom_Evo#Revvity_Chemagic_360|Chemagic360]])&lt;br /&gt;
* Quality Control&amp;lt;BR&amp;gt;([[BioMicroCenter:QC|AATI Fragment Analyzer/FemtoPulse]])&lt;br /&gt;
 |}&lt;br /&gt;
 |valign=&amp;quot;top&amp;quot; align=&amp;quot;center&amp;quot; style=&amp;quot;background-color:transparent; border:1px solid black&amp;quot;| &lt;br /&gt;
 {|&lt;br /&gt;
  |width=&amp;quot;10%&amp;quot; align=&amp;quot;left&amp;quot;|&amp;lt;BIG&amp;gt;&amp;lt;B&amp;gt;Library Preparation&amp;lt;/B&amp;gt;&amp;lt;/BIG&amp;gt;&lt;br /&gt;
* [[BioMicroCenter:Illumina_Library_Preparation|Short Read Sample Preparation]]&amp;lt;small&amp;gt;&lt;br /&gt;
** Standard Methods for [[BioMicroCenter:DNA_LIB| *DNA* ]] [[BioMicroCenter:RNA_LIB| *RNA* ]]&lt;br /&gt;
** [[BioMicroCenter:FAQ#STANDARD_versus_HIGH_THROUGHPUT_LIBRARY_PREPARATION|High Throughput Methods]] for [[BioMicroCenter:DNA_HTL| *DNA* ]]  [[BioMicroCenter:RNA_HTL| *RNA* ]]&amp;lt;/small&amp;gt;&lt;br /&gt;
* Long Read Sample Preparation&amp;lt;BR&amp;gt;([[BioMicroCenter:NanoPore_Library_Prep|Nanopore]] and [[BioMicroCenter:PacBio_Library_Preparation|PacBio]])&lt;br /&gt;
 |}&lt;br /&gt;
|valign=&amp;quot;top&amp;quot; align=&amp;quot;center&amp;quot; style=&amp;quot;background-color:transparent; border:1px solid black&amp;quot;| &lt;br /&gt;
 {|&lt;br /&gt;
  |width=&amp;quot;10%&amp;quot; align=&amp;quot;left&amp;quot;|&amp;lt;BIG&amp;gt;&amp;lt;B&amp;gt;[[BioMicroCenter:Walkup Services|Walk-up Instrumentation]]&amp;lt;/B&amp;gt;&amp;lt;/BIG&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;Includes: qPCR (LC480), Plate Readers (Varioskan/Synergy H1)&amp;lt;BR&amp;gt; BioAnalyzer, NanoDrop, Covaris, Pippin Prep&amp;lt;BR&amp;gt; Tecan/Chemagic automation&amp;lt;BR&amp;gt; MiSeq i100, 10x Chromium X&amp;lt;/small&amp;gt;&lt;br /&gt;
 |}&lt;br /&gt;
&lt;br /&gt;
 |-&lt;br /&gt;
 |valign=&amp;quot;top&amp;quot; align=&amp;quot;center&amp;quot; style=&amp;quot;background-color:transparent; border:1px solid black&amp;quot;| &lt;br /&gt;
 {|&lt;br /&gt;
  |width=&amp;quot;10%&amp;quot; align=&amp;quot;left&amp;quot;|&amp;lt;BIG&amp;gt;&amp;lt;B&amp;gt; &lt;br /&gt;
[[BioMicroCenter:SingleCell|Single Cell]] and [[BioMicroCenter:SpTx|Spatial]]&amp;lt;/B&amp;gt;&amp;lt;/BIG&amp;gt;&lt;br /&gt;
* 10X CHROMIUM &amp;lt;BR&amp;gt; &amp;lt;small&amp;gt; (5&#039;RNA, 3&#039;RNA, ATAC, multiome) &amp;lt;/small&amp;gt;&lt;br /&gt;
* 10X VISIUM &amp;lt;BR&amp;gt; &amp;lt;small&amp;gt; (Standard, High Density) &amp;lt;/small&amp;gt;&lt;br /&gt;
* ELEMENT AVITI24 (&amp;lt;i&amp;gt;in situ&amp;lt;/i&amp;gt; prep/sequencing)&lt;br /&gt;
 |}&lt;br /&gt;
 |valign=&amp;quot;top&amp;quot; align=&amp;quot;center&amp;quot; style=&amp;quot;background-color:transparent; border:1px solid black&amp;quot;| &lt;br /&gt;
 {|&lt;br /&gt;
  |width=&amp;quot;10%&amp;quot; align=&amp;quot;left&amp;quot;|&amp;lt;BIG&amp;gt;&amp;lt;B&amp;gt; [[BioMicroCenter:Sequencing|Sequencing Technologies]] &amp;lt;/B&amp;gt;&amp;lt;/BIG&amp;gt;&lt;br /&gt;
* [[BioMicroCenter:ShortRead|Short Read]]&lt;br /&gt;
** High Yield (&amp;gt;1b reads): Illumina NovaSeqX (25b,10b)†&lt;br /&gt;
** Mid Yield (250m-1b reads): Element AVITI24&lt;br /&gt;
** Low Yield (1m-150m reads): SingularG4, Illumina MiSeq i100&lt;br /&gt;
* Long Read (1kb+) &amp;lt;small&amp;gt;&lt;br /&gt;
**[[BioMicroCenter:Oxford Nanopore Technologies|ONT PromethION]] &lt;br /&gt;
**[[BioMicroCenter:PacBio|PacBio Revio]]†&amp;lt;/small&amp;gt;&lt;br /&gt;
 |}&lt;br /&gt;
 |valign=&amp;quot;top&amp;quot; align=&amp;quot;center&amp;quot; style=&amp;quot;background-color:transparent; border:1px solid black&amp;quot;| &lt;br /&gt;
 {|&lt;br /&gt;
  |width=&amp;quot;10%&amp;quot; align=&amp;quot;left&amp;quot;|&amp;lt;BIG&amp;gt;&amp;lt;B&amp;gt;Automation / Library Prep&amp;lt;/B&amp;gt;&amp;lt;/BIG&amp;gt;&lt;br /&gt;
* [[BioMicroCenter:Tecan Freedom Evo|Liquid Handlers]] ([[BioMicroCenter:Tecan_Freedom_Evo#JOHNNY_5|Tecan EVO]]&lt;br /&gt;
* [[BioMicroCenter:Tecan_Freedom_Evo#SPT_LABTECH_MOSQUITO_HV|SPT Mosquito]])&lt;br /&gt;
* [[BioMicroCenter:Covaris|Covaris E220 and R230 Focused-ultrasonicators]]&lt;br /&gt;
* [[BioMicroCenter:PippinPrep|Pippin Prep automated electrophoresis system]]&lt;br /&gt;
* [[BioMicroCenter:Oligo_Synthesis| Oligo Synthesis]]&lt;br /&gt;
 |}&lt;br /&gt;
 |- &lt;br /&gt;
 |valign=&amp;quot;top&amp;quot; colspan=3 align=&amp;quot;center&amp;quot; style=&amp;quot;background-color: lightsalmon; border:1px solid black&amp;quot;| &lt;br /&gt;
 {|&lt;br /&gt;
  |&amp;lt;BIG&amp;gt;&amp;lt;B&amp;gt;Informatics Services&amp;lt;/B&amp;gt;&amp;lt;/BIG&amp;gt;&lt;br /&gt;
 |}&lt;br /&gt;
 |-&lt;br /&gt;
 |valign=&amp;quot;top&amp;quot; align=&amp;quot;center&amp;quot; style=&amp;quot;background-color:transparent; border:1px solid black&amp;quot;| &lt;br /&gt;
 {|&lt;br /&gt;
  |width=&amp;quot;10%&amp;quot; align=&amp;quot;left&amp;quot;|&amp;lt;BIG&amp;gt;&amp;lt;B&amp;gt;[[BioMicroCenter:Analysis|Data Analysis]]&amp;lt;/B&amp;gt;&amp;lt;/BIG&amp;gt;&lt;br /&gt;
* [https://igb.mit.edu/ Bioinformatic Consulting]&lt;br /&gt;
* [[BioMicroCenter:People#INFORMATICS_LAB|Informatics Staff]]&lt;br /&gt;
 |}&lt;br /&gt;
 |valign=&amp;quot;top&amp;quot; align=&amp;quot;center&amp;quot; style=&amp;quot;background-color:transparent; border:1px solid black&amp;quot;| &lt;br /&gt;
 {|&lt;br /&gt;
  |width=&amp;quot;10%&amp;quot; align=&amp;quot;left&amp;quot;|&amp;lt;BIG&amp;gt;&amp;lt;B&amp;gt;BioIT/Research Computing&amp;lt;/B&amp;gt;&amp;lt;/BIG&amp;gt;&lt;br /&gt;
* [https://koch-institute-mit.gitbook.io/mit-data-management-analysis-core/ FAIR Data Management]&lt;br /&gt;
* [https://igb.mit.edu/computing-resources/active-data-storage Data Storage]&lt;br /&gt;
* [https://igb.mit.edu/computing-resources/luria-cluster Computing on Luria]&lt;br /&gt;
* [https://igb.mit.edu/data-management/globus Data Transfer (Globus)]&lt;br /&gt;
 |}&lt;br /&gt;
 |valign=&amp;quot;top&amp;quot; align=&amp;quot;center&amp;quot; style=&amp;quot;background-color:transparent; border:1px solid black&amp;quot;| &lt;br /&gt;
 {|&lt;br /&gt;
  |width=&amp;quot;10%&amp;quot; align=&amp;quot;left&amp;quot;|&amp;lt;BIG&amp;gt;&amp;lt;B&amp;gt;Education/Training/Seminars&amp;lt;/B&amp;gt;&amp;lt;/BIG&amp;gt;&lt;br /&gt;
* [https://igb.mit.edu/mini-courses Informatics/Computing Training Sessions]&lt;br /&gt;
* [[BioMicroCenter:Technology_Seminar_Series|Technology Seminar Series]]&lt;br /&gt;
* [http://mailman.mit.edu:/mailman/listinfo/biostuff Biostuff mailing list]&lt;br /&gt;
 |}&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
† - service will be performed in a sister core facility (Harvard, DFCI, or Broad)&amp;lt;BR&amp;gt;&lt;br /&gt;
&amp;lt;BR&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
[https://wikis.mit.edu/confluence/display/bmc &#039;&#039;BMC Internal wiki&#039;&#039;]&lt;br /&gt;
&lt;br /&gt;
{{Footer}}&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=Template:Footer&amp;diff=131562</id>
		<title>Template:Footer</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=Template:Footer&amp;diff=131562"/>
		<updated>2026-06-01T17:49:42Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;&amp;lt;div id=&amp;quot;footer&amp;quot; style=&amp;quot;display: flex; flex-direction: row; justify-content: space-evenly;&amp;quot;&amp;gt;&lt;br /&gt;
&amp;lt;div class=&amp;quot;bmc-footer-col&amp;quot;&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;h4 id=&amp;quot;footer-title&amp;quot;&amp;gt;MIT BioMicro Center&amp;lt;/h4&amp;gt;&lt;br /&gt;
* Building [https://whereis.mit.edu/?go=68 68-322]&lt;br /&gt;
* Cambridge, MA 02139&lt;br /&gt;
* [mailto:biomicro@mit.edu biomicro@mit.edu]&lt;br /&gt;
* 617-715-4533&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div class=&amp;quot;bmc-footer-col&amp;quot;&amp;gt;&lt;br /&gt;
==== Services ====&lt;br /&gt;
* [[BioMicroCenter:Sequencing | Bulk Sequencing]]&lt;br /&gt;
* [[BioMicroCenter:SingleCell | Single Cell]]&lt;br /&gt;
* [[BioMicroCenter:SpTx | Spatial Genomics]]&lt;br /&gt;
* [https://igb.mit.edu Informatics]&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div class=&amp;quot;bmc-footer-col&amp;quot;&amp;gt;&lt;br /&gt;
==== Resources ====&lt;br /&gt;
* [[BioMicroCenter:FAQ | FAQs]]&lt;br /&gt;
* [[BioMicroCenter:Consulting | Consulting]]&lt;br /&gt;
* [[BioMicroCenter:Pricing | Grant Support]]&lt;br /&gt;
* [[BioMicroCenter:Acknowledgement | Acknowledgement]]&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=Template:Footer&amp;diff=131561</id>
		<title>Template:Footer</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=Template:Footer&amp;diff=131561"/>
		<updated>2026-06-01T17:45:48Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;&amp;lt;div id=&amp;quot;footer&amp;quot;&amp;gt;&lt;br /&gt;
&amp;lt;div class=&amp;quot;bmc-footer-col&amp;quot;&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;h4 id=&amp;quot;footer-title&amp;quot;&amp;gt;MIT BioMicro Center&amp;lt;/h4&amp;gt;&lt;br /&gt;
* Building [https://whereis.mit.edu/?go=68 68-322]&lt;br /&gt;
* Cambridge, MA 02139&lt;br /&gt;
* [mailto:biomicro@mit.edu biomicro@mit.edu]&lt;br /&gt;
* 617-715-4533&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div class=&amp;quot;bmc-footer-col&amp;quot;&amp;gt;&lt;br /&gt;
==== Services ====&lt;br /&gt;
* [[BioMicroCenter:Sequencing | Bulk Sequencing]]&lt;br /&gt;
* [[BioMicroCenter:SingleCell | Single Cell]]&lt;br /&gt;
* [[BioMicroCenter:SpTx | Spatial Genomics]]&lt;br /&gt;
* [https://igb.mit.edu Informatics]&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div class=&amp;quot;bmc-footer-col&amp;quot;&amp;gt;&lt;br /&gt;
==== Resources ====&lt;br /&gt;
* [[BioMicroCenter:FAQ | FAQs]]&lt;br /&gt;
* [[BioMicroCenter:Consulting | Consulting]]&lt;br /&gt;
* [[BioMicroCenter:Pricing | Grant Support]]&lt;br /&gt;
* [[BioMicroCenter:Acknowledgement | Acknowledgement]]&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=Template:Footer&amp;diff=131560</id>
		<title>Template:Footer</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=Template:Footer&amp;diff=131560"/>
		<updated>2026-06-01T17:40:02Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;&amp;lt;div id=&amp;quot;footer&amp;quot;&amp;gt;&lt;br /&gt;
&amp;lt;div class=&amp;quot;bmc-footer-col&amp;quot;&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;h4 id=&amp;quot;footer-title&amp;quot;&amp;gt;MIT BioMicro Center&amp;lt;/h4&amp;gt;&lt;br /&gt;
* Building 68-322&lt;br /&gt;
* Cambridge, MA 02139&lt;br /&gt;
* [mailto:biomicro@mit.edu biomicro@mit.edu]&lt;br /&gt;
* 617-715-4533&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div class=&amp;quot;bmc-footer-col&amp;quot;&amp;gt;&lt;br /&gt;
==== Services ====&lt;br /&gt;
* [[BioMicroCenter:Sequencing | Bulk Sequencing]]&lt;br /&gt;
* [[BioMicroCenter:SingleCell | Single Cell]]&lt;br /&gt;
* [[BioMicroCenter:SpTx | Spatial Genomics]]&lt;br /&gt;
* [https://igb.mit.edu Informatics]&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div class=&amp;quot;bmc-footer-col&amp;quot;&amp;gt;&lt;br /&gt;
==== Resources ====&lt;br /&gt;
* [[BioMicroCenter:FAQ | FAQs]]&lt;br /&gt;
* [[BioMicroCenter:Consulting | Consulting]]&lt;br /&gt;
* [[BioMicroCenter:Pricing | Grant Support]]&lt;br /&gt;
* [[BioMicroCenter:Acknowledgement | Acknowledgement]]&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=Template:Footer&amp;diff=131556</id>
		<title>Template:Footer</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=Template:Footer&amp;diff=131556"/>
		<updated>2026-06-01T16:23:33Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;&amp;lt;div id=&amp;quot;footer&amp;quot;&amp;gt;&lt;br /&gt;
&amp;lt;h4 id=&amp;quot;footer-title&amp;quot;&amp;gt;MIT BioMicro Center&amp;lt;/h4&amp;gt;&lt;br /&gt;
&lt;br /&gt;
* Building 68-322&lt;br /&gt;
* Cambridge, MA 02139&lt;br /&gt;
* [mailto:biomicro@mit.edu biomicro@mit.edu]&lt;br /&gt;
* 617-715-4533&lt;br /&gt;
&lt;br /&gt;
==== Services ====&lt;br /&gt;
* [[BioMicroCenter:Sequencing | Bulk Sequencing]]&lt;br /&gt;
* [[BioMicroCenter:SingleCell | Single Cell]]&lt;br /&gt;
* [[BioMicroCenter:SpTx | Spatial Genomics]]&lt;br /&gt;
* [https://igb.mit.edu Informatics]&lt;br /&gt;
&lt;br /&gt;
==== Resources ====&lt;br /&gt;
* [[BioMicroCenter:FAQ | FAQs]]&lt;br /&gt;
* [[BioMicroCenter:Consulting | Consulting]]&lt;br /&gt;
* [[BioMicroCenter:Pricing | Grant Support]]&lt;br /&gt;
* [[BioMicroCenter:Acknowledgement | Acknowledgement]]&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=Template:Footer&amp;diff=131555</id>
		<title>Template:Footer</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=Template:Footer&amp;diff=131555"/>
		<updated>2026-06-01T16:23:24Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;&amp;lt;div id=&amp;quot;footer&amp;quot;&amp;gt;&lt;br /&gt;
&amp;lt;h4 id=&amp;quot;footer-title&amp;quot;&amp;gt;MIT BioMicro Center&amp;lt;/h4&amp;gt;&lt;br /&gt;
&lt;br /&gt;
* Building 68-322&lt;br /&gt;
* Cambridge, MA 02139&lt;br /&gt;
* [mailto:biomicro@mit.edu biomicro@mit.edu]&lt;br /&gt;
* 617-715-4533&lt;br /&gt;
&lt;br /&gt;
==== Services ====&lt;br /&gt;
* [[BioMicroCenter:Sequencing | Bulk Sequencing]]&lt;br /&gt;
* [[BioMicroCenter:SingleCell | Single Cell]]&lt;br /&gt;
* [[BioMicroCenter:SpTx | Spatial Genomics]]&lt;br /&gt;
* [https://igb.mit.edu | Informatics]&lt;br /&gt;
&lt;br /&gt;
==== Resources ====&lt;br /&gt;
* [[BioMicroCenter:FAQ | FAQs]]&lt;br /&gt;
* [[BioMicroCenter:Consulting | Consulting]]&lt;br /&gt;
* [[BioMicroCenter:Pricing | Grant Support]]&lt;br /&gt;
* [[BioMicroCenter:Acknowledgement | Acknowledgement]]&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=Template:Footer&amp;diff=131554</id>
		<title>Template:Footer</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=Template:Footer&amp;diff=131554"/>
		<updated>2026-06-01T16:10:56Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: Created page with &amp;quot;&amp;lt;div id=&amp;quot;footer&amp;quot;&amp;gt; &amp;lt;h4 id=&amp;quot;footer-title&amp;quot;&amp;gt;MIT BioMicro Center&amp;lt;/h4&amp;gt;  * Building 68-322 * Cambridge, MA 02139 * [mailto:biomicro@mit.edu biomicro@mit.edu] * 617-715-4533  ==== Services ==== *  Bulk Sequencing *  Consulting *  Grant Support *  Acknowledgement &amp;lt;/div&amp;gt;&amp;quot;&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;&amp;lt;div id=&amp;quot;footer&amp;quot;&amp;gt;&lt;br /&gt;
&amp;lt;h4 id=&amp;quot;footer-title&amp;quot;&amp;gt;MIT BioMicro Center&amp;lt;/h4&amp;gt;&lt;br /&gt;
&lt;br /&gt;
* Building 68-322&lt;br /&gt;
* Cambridge, MA 02139&lt;br /&gt;
* [mailto:biomicro@mit.edu biomicro@mit.edu]&lt;br /&gt;
* 617-715-4533&lt;br /&gt;
&lt;br /&gt;
==== Services ====&lt;br /&gt;
* [[BioMicroCenter:Sequencing | Bulk Sequencing]]&lt;br /&gt;
* [[BioMicroCenter:Consulting | Consulting]]&lt;br /&gt;
* [[BioMicroCenter:Pricing | Grant Support]]&lt;br /&gt;
* [[BioMicroCenter:Acknowledgement | Acknowledgement]]&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=User:Asoberan/common.js&amp;diff=131553</id>
		<title>User:Asoberan/common.js</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=User:Asoberan/common.js&amp;diff=131553"/>
		<updated>2026-06-01T16:00:44Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: Blanked the page&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=User:Asoberan/common.css&amp;diff=131552</id>
		<title>User:Asoberan/common.css</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=User:Asoberan/common.css&amp;diff=131552"/>
		<updated>2026-06-01T16:00:29Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: Blanked the page&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=User:Asoberan/common.js&amp;diff=131551</id>
		<title>User:Asoberan/common.js</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=User:Asoberan/common.js&amp;diff=131551"/>
		<updated>2026-06-01T15:54:24Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: Created page with &amp;quot;/**  * BioMicro Center Wiki — Full DOM Replacement  * Paste into: User:USERNAME/common.js  *  * Strategy:  * 1. Extract wiki article content from MediaWiki&amp;#039;s DOM  * 2. Inject the complete shell structure  * 3. Place article content inside the shell  * MediaWiki still runs underneath for editing, search, login, etc.  */  ( function () {   &amp;#039;use strict&amp;#039;;    /* ── Extract nav from MediaWiki&amp;#039;s sidebar DOM ─────────────── */   function extra...&amp;quot;&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;/**&lt;br /&gt;
 * BioMicro Center Wiki — Full DOM Replacement&lt;br /&gt;
 * Paste into: User:USERNAME/common.js&lt;br /&gt;
 *&lt;br /&gt;
 * Strategy:&lt;br /&gt;
 * 1. Extract wiki article content from MediaWiki&#039;s DOM&lt;br /&gt;
 * 2. Inject the complete shell structure&lt;br /&gt;
 * 3. Place article content inside the shell&lt;br /&gt;
 * MediaWiki still runs underneath for editing, search, login, etc.&lt;br /&gt;
 */&lt;br /&gt;
&lt;br /&gt;
( function () {&lt;br /&gt;
  &#039;use strict&#039;;&lt;br /&gt;
&lt;br /&gt;
  /* ── Extract nav from MediaWiki&#039;s sidebar DOM ─────────────── */&lt;br /&gt;
  function extractNavFromDom() {&lt;br /&gt;
    var SKIP_IDS = {&lt;br /&gt;
      &#039;p-tb&#039;: 1, &#039;p-personal&#039;: 1, &#039;p-search&#039;: 1,&lt;br /&gt;
      &#039;p-logo&#039;: 1, &#039;p-cactions&#039;: 1, &#039;p-views&#039;: 1,&lt;br /&gt;
      &#039;p-lang&#039;: 1, &#039;p-namespaces&#039;: 1&lt;br /&gt;
    };&lt;br /&gt;
&lt;br /&gt;
    var portlets = Array.prototype.slice.call(&lt;br /&gt;
      document.querySelectorAll( &#039;[id^=&amp;quot;p-&amp;quot;]&#039; )&lt;br /&gt;
    );&lt;br /&gt;
&lt;br /&gt;
    var nav = [];&lt;br /&gt;
    var currentUrl = window.location.href.split( &#039;?&#039; )[0];&lt;br /&gt;
&lt;br /&gt;
    portlets.forEach( function ( portlet ) {&lt;br /&gt;
      if ( SKIP_IDS[ portlet.id ] ) return;&lt;br /&gt;
&lt;br /&gt;
      var links = Array.prototype.slice.call(&lt;br /&gt;
        portlet.querySelectorAll( &#039;li a&#039; )&lt;br /&gt;
      ).map( function ( a ) {&lt;br /&gt;
        return { label: a.textContent.trim(), href: a.href };&lt;br /&gt;
      } ).filter( function ( item ) { return item.label &amp;amp;&amp;amp; item.href; } );&lt;br /&gt;
&lt;br /&gt;
      if ( !links.length ) return;&lt;br /&gt;
&lt;br /&gt;
      var headingEl = portlet.querySelector( &#039;[id$=&amp;quot;-label&amp;quot;], h3, h2&#039; );&lt;br /&gt;
      var sectionLabel = headingEl ? headingEl.textContent.trim() : &#039;&#039;;&lt;br /&gt;
&lt;br /&gt;
      var isActive = links.some( function ( link ) {&lt;br /&gt;
        return link.href.split( &#039;?&#039; )[0] === currentUrl;&lt;br /&gt;
      } );&lt;br /&gt;
&lt;br /&gt;
      if ( links.length === 1 ) {&lt;br /&gt;
        nav.push( {&lt;br /&gt;
          label:    links[0].label,&lt;br /&gt;
          href:     links[0].href,&lt;br /&gt;
          isActive: links[0].href.split( &#039;?&#039; )[0] === currentUrl&lt;br /&gt;
        } );&lt;br /&gt;
      } else {&lt;br /&gt;
        nav.push( {&lt;br /&gt;
          label:    sectionLabel || links[0].label,&lt;br /&gt;
          href:     links[0].href,&lt;br /&gt;
          items:    links,&lt;br /&gt;
          isActive: isActive&lt;br /&gt;
        } );&lt;br /&gt;
      }&lt;br /&gt;
    } );&lt;br /&gt;
&lt;br /&gt;
    return nav;&lt;br /&gt;
  }&lt;br /&gt;
&lt;br /&gt;
  /* ── Build topbar HTML ─────────────────────────────────────── */&lt;br /&gt;
  function buildTopbar() {&lt;br /&gt;
    var d = document.createElement( &#039;div&#039; );&lt;br /&gt;
    d.className = &#039;bmc-topbar&#039;;&lt;br /&gt;
    d.innerHTML =&lt;br /&gt;
      &#039;&amp;lt;div class=&amp;quot;inner&amp;quot;&amp;gt;&#039; +&lt;br /&gt;
        &#039;&amp;lt;a href=&amp;quot;mailto:biomicro@mit.edu&amp;quot;&amp;gt;biomicro@mit.edu&amp;lt;/a&amp;gt;&#039; +&lt;br /&gt;
        &#039;&amp;lt;span&amp;gt;|&amp;lt;/span&amp;gt;&#039; +&lt;br /&gt;
        &#039;617-715-4533&#039; +&lt;br /&gt;
        &#039;&amp;lt;span&amp;gt;|&amp;lt;/span&amp;gt;&#039; +&lt;br /&gt;
        &#039;Building 68-322&#039; +&lt;br /&gt;
      &#039;&amp;lt;/div&amp;gt;&#039;;&lt;br /&gt;
    return d;&lt;br /&gt;
  }&lt;br /&gt;
&lt;br /&gt;
  /* ── Build nav dropdown item ───────────────────────────────── */&lt;br /&gt;
  function buildDropdownMenu( items ) {&lt;br /&gt;
    var menu = document.createElement( &#039;div&#039; );&lt;br /&gt;
    menu.className = &#039;bmc-dropdown-menu&#039;;&lt;br /&gt;
    items.forEach( function ( item ) {&lt;br /&gt;
      if ( item.divider ) {&lt;br /&gt;
        var div = document.createElement( &#039;div&#039; );&lt;br /&gt;
        div.className = &#039;bmc-divider&#039;;&lt;br /&gt;
        menu.appendChild( div );&lt;br /&gt;
      } else if ( item.groupLabel ) {&lt;br /&gt;
        var gl = document.createElement( &#039;div&#039; );&lt;br /&gt;
        gl.className = &#039;bmc-group-label&#039;;&lt;br /&gt;
        gl.textContent = item.groupLabel;&lt;br /&gt;
        menu.appendChild( gl );&lt;br /&gt;
      } else {&lt;br /&gt;
        var a = document.createElement( &#039;a&#039; );&lt;br /&gt;
        a.href = item.page ? mw.util.getUrl( item.page ) : item.href;&lt;br /&gt;
        a.textContent = item.label;&lt;br /&gt;
        if ( item.ext ) a.target = &#039;_blank&#039;;&lt;br /&gt;
        menu.appendChild( a );&lt;br /&gt;
      }&lt;br /&gt;
    } );&lt;br /&gt;
    return menu;&lt;br /&gt;
  }&lt;br /&gt;
&lt;br /&gt;
  /* ── Build full header ─────────────────────────────────────── */&lt;br /&gt;
  function buildHeader( currentPage, nav ) {&lt;br /&gt;
    /* Logo */&lt;br /&gt;
    var logo = document.createElement( &#039;a&#039; );&lt;br /&gt;
    logo.href = mw.util.getUrl( &#039;BioMicroCenter&#039; );&lt;br /&gt;
    logo.className = &#039;bmc-logo&#039;;&lt;br /&gt;
    logo.innerHTML =&lt;br /&gt;
      &#039;&amp;lt;img class=&amp;quot;bmc-logo-img&amp;quot; src=&amp;quot;https://bmcwiki.mit.edu/images/c/c9/Logo.png&amp;quot; alt=&amp;quot;MIT BioMicro Center&amp;quot; /&amp;gt;&#039;;&lt;br /&gt;
&lt;br /&gt;
    /* Nav */&lt;br /&gt;
    var ul = document.createElement( &#039;ul&#039; );&lt;br /&gt;
    nav.forEach( function ( item ) {&lt;br /&gt;
      var li = document.createElement( &#039;li&#039; );&lt;br /&gt;
      if ( item.isActive ) li.classList.add( &#039;bmc-active&#039; );&lt;br /&gt;
      if ( item.items ) li.classList.add( &#039;bmc-dropdown&#039; );&lt;br /&gt;
&lt;br /&gt;
      var a = document.createElement( &#039;a&#039; );&lt;br /&gt;
      a.href = item.page ? mw.util.getUrl( item.page ) : ( item.href || &#039;#&#039; );&lt;br /&gt;
      a.textContent = item.label;&lt;br /&gt;
      li.appendChild( a );&lt;br /&gt;
&lt;br /&gt;
      if ( item.items ) {&lt;br /&gt;
        li.appendChild( buildDropdownMenu( item.items ) );&lt;br /&gt;
      }&lt;br /&gt;
      ul.appendChild( li );&lt;br /&gt;
    } );&lt;br /&gt;
&lt;br /&gt;
    var navEl = document.createElement( &#039;nav&#039; );&lt;br /&gt;
    navEl.className = &#039;bmc-nav&#039;;&lt;br /&gt;
    navEl.appendChild( ul );&lt;br /&gt;
&lt;br /&gt;
    /* Search */&lt;br /&gt;
    var searchForm = document.createElement( &#039;form&#039; );&lt;br /&gt;
    searchForm.className = &#039;bmc-search-form&#039;;&lt;br /&gt;
    searchForm.method = &#039;get&#039;;&lt;br /&gt;
    searchForm.action = &#039;/index.php&#039;;&lt;br /&gt;
    searchForm.innerHTML =&lt;br /&gt;
      &#039;&amp;lt;input type=&amp;quot;hidden&amp;quot; name=&amp;quot;title&amp;quot; value=&amp;quot;Special:Search&amp;quot;&amp;gt;&#039; +&lt;br /&gt;
      &#039;&amp;lt;input type=&amp;quot;search&amp;quot; name=&amp;quot;search&amp;quot; placeholder=&amp;quot;Search wiki…&amp;quot; aria-label=&amp;quot;Search&amp;quot;&amp;gt;&#039; +&lt;br /&gt;
      &#039;&amp;lt;button type=&amp;quot;submit&amp;quot; aria-label=&amp;quot;Search&amp;quot;&amp;gt;&amp;lt;svg xmlns=&amp;quot;http://www.w3.org/2000/svg&amp;quot; width=&amp;quot;14&amp;quot; height=&amp;quot;14&amp;quot; viewBox=&amp;quot;0 0 24 24&amp;quot; fill=&amp;quot;none&amp;quot; stroke=&amp;quot;currentColor&amp;quot; stroke-width=&amp;quot;2.5&amp;quot; stroke-linecap=&amp;quot;round&amp;quot; stroke-linejoin=&amp;quot;round&amp;quot;&amp;gt;&amp;lt;circle cx=&amp;quot;11&amp;quot; cy=&amp;quot;11&amp;quot; r=&amp;quot;8&amp;quot;/&amp;gt;&amp;lt;line x1=&amp;quot;21&amp;quot; y1=&amp;quot;21&amp;quot; x2=&amp;quot;16.65&amp;quot; y2=&amp;quot;16.65&amp;quot;/&amp;gt;&amp;lt;/svg&amp;gt;&amp;lt;/button&amp;gt;&#039;;&lt;br /&gt;
&lt;br /&gt;
    var right = document.createElement( &#039;div&#039; );&lt;br /&gt;
    right.className = &#039;bmc-header-right&#039;;&lt;br /&gt;
    right.appendChild( navEl );&lt;br /&gt;
    right.appendChild( searchForm );&lt;br /&gt;
&lt;br /&gt;
    /* Inner wrapper */&lt;br /&gt;
    var inner = document.createElement( &#039;div&#039; );&lt;br /&gt;
    inner.className = &#039;bmc-header-inner&#039;;&lt;br /&gt;
    inner.appendChild( logo );&lt;br /&gt;
    inner.appendChild( right );&lt;br /&gt;
&lt;br /&gt;
    var header = document.createElement( &#039;header&#039; );&lt;br /&gt;
    header.className = &#039;bmc-header&#039;;&lt;br /&gt;
    header.appendChild( inner );&lt;br /&gt;
    return header;&lt;br /&gt;
  }&lt;br /&gt;
&lt;br /&gt;
  /* ── Build page hero ───────────────────────────────────────── */&lt;br /&gt;
  function buildHero( titleText ) {&lt;br /&gt;
    var crumb = document.createElement( &#039;div&#039; );&lt;br /&gt;
    crumb.className = &#039;bmc-breadcrumb&#039;;&lt;br /&gt;
    crumb.innerHTML =&lt;br /&gt;
      &#039;&amp;lt;a href=&amp;quot;&#039; + mw.util.getUrl( &#039;BioMicroCenter&#039; ) + &#039;&amp;quot;&amp;gt;Home&amp;lt;/a&amp;gt;&#039; +&lt;br /&gt;
      &#039;&amp;lt;span class=&amp;quot;sep&amp;quot;&amp;gt;›&amp;lt;/span&amp;gt;&#039; +&lt;br /&gt;
      document.createTextNode( titleText ).textContent; // plain text, no XSS&lt;br /&gt;
&lt;br /&gt;
    var h1 = document.createElement( &#039;h1&#039; );&lt;br /&gt;
    h1.textContent = titleText;&lt;br /&gt;
&lt;br /&gt;
    var inner = document.createElement( &#039;div&#039; );&lt;br /&gt;
    inner.className = &#039;inner&#039;;&lt;br /&gt;
    inner.appendChild( crumb );&lt;br /&gt;
    inner.appendChild( h1 );&lt;br /&gt;
&lt;br /&gt;
    var hero = document.createElement( &#039;div&#039; );&lt;br /&gt;
    hero.className = &#039;bmc-page-hero&#039;;&lt;br /&gt;
    hero.appendChild( inner );&lt;br /&gt;
    return hero;&lt;br /&gt;
  }&lt;br /&gt;
&lt;br /&gt;
  /* ── Build sidebar TOC from MediaWiki #toc or heading scan ─── */&lt;br /&gt;
  function buildSidebarToc( mwToc, contentEl ) {&lt;br /&gt;
    var toc = document.createElement( &#039;div&#039; );&lt;br /&gt;
    toc.className = &#039;bmc-toc&#039;;&lt;br /&gt;
&lt;br /&gt;
    var h3 = document.createElement( &#039;h3&#039; );&lt;br /&gt;
    h3.textContent = &#039;On this page&#039;;&lt;br /&gt;
    toc.appendChild( h3 );&lt;br /&gt;
&lt;br /&gt;
    var list;&lt;br /&gt;
&lt;br /&gt;
    if ( mwToc ) {&lt;br /&gt;
      /* Clone only the list from the MediaWiki TOC */&lt;br /&gt;
      var mwList = mwToc.querySelector( &#039;ul&#039; );&lt;br /&gt;
      if ( mwList ) {&lt;br /&gt;
        list = mwList.cloneNode( true );&lt;br /&gt;
        /* Mark sub-items */&lt;br /&gt;
        list.querySelectorAll( &#039;li li&#039; ).forEach( function ( li ) {&lt;br /&gt;
          li.classList.add( &#039;bmc-sub&#039; );&lt;br /&gt;
        } );&lt;br /&gt;
      }&lt;br /&gt;
    } else if ( contentEl ) {&lt;br /&gt;
      /* Fall back to scanning h2/h3 headings directly */&lt;br /&gt;
      var headings = Array.prototype.slice.call(&lt;br /&gt;
        contentEl.querySelectorAll( &#039;h2, h3&#039; )&lt;br /&gt;
      );&lt;br /&gt;
      if ( headings.length &amp;gt; 0 ) {&lt;br /&gt;
        list = document.createElement( &#039;ul&#039; );&lt;br /&gt;
        headings.forEach( function ( heading ) {&lt;br /&gt;
          var anchor = heading.querySelector( &#039;.mw-headline&#039; );&lt;br /&gt;
          if ( !anchor || !anchor.id ) return;&lt;br /&gt;
          var li = document.createElement( &#039;li&#039; );&lt;br /&gt;
          if ( heading.tagName === &#039;H3&#039; ) li.classList.add( &#039;bmc-sub&#039; );&lt;br /&gt;
          var a = document.createElement( &#039;a&#039; );&lt;br /&gt;
          a.href = &#039;#&#039; + anchor.id;&lt;br /&gt;
          a.textContent = anchor.textContent.trim();&lt;br /&gt;
          li.appendChild( a );&lt;br /&gt;
          list.appendChild( li );&lt;br /&gt;
        } );&lt;br /&gt;
      }&lt;br /&gt;
    }&lt;br /&gt;
&lt;br /&gt;
    if ( !list || list.childNodes.length === 0 ) return null;&lt;br /&gt;
&lt;br /&gt;
    toc.appendChild( list );&lt;br /&gt;
&lt;br /&gt;
    var aside = document.createElement( &#039;aside&#039; );&lt;br /&gt;
    aside.className = &#039;bmc-sidebar&#039;;&lt;br /&gt;
    aside.appendChild( toc );&lt;br /&gt;
    return aside;&lt;br /&gt;
  }&lt;br /&gt;
&lt;br /&gt;
  /* ── Build footer ──────────────────────────────────────────── */&lt;br /&gt;
  function buildFooter() {&lt;br /&gt;
    var u = mw.util.getUrl;&lt;br /&gt;
    var inner = document.createElement( &#039;div&#039; );&lt;br /&gt;
    inner.className = &#039;bmc-footer-inner&#039;;&lt;br /&gt;
    inner.innerHTML =&lt;br /&gt;
      &#039;&amp;lt;div&amp;gt;&#039; +&lt;br /&gt;
        &#039;&amp;lt;h4&amp;gt;MIT BioMicro Center&amp;lt;/h4&amp;gt;&#039; +&lt;br /&gt;
        &#039;&amp;lt;ul&amp;gt;&#039; +&lt;br /&gt;
          &#039;&amp;lt;li&amp;gt;Building 68-322&amp;lt;/li&amp;gt;&#039; +&lt;br /&gt;
          &#039;&amp;lt;li&amp;gt;Cambridge, MA 02139&amp;lt;/li&amp;gt;&#039; +&lt;br /&gt;
          &#039;&amp;lt;li&amp;gt;&amp;lt;a href=&amp;quot;mailto:biomicro@mit.edu&amp;quot;&amp;gt;biomicro@mit.edu&amp;lt;/a&amp;gt;&amp;lt;/li&amp;gt;&#039; +&lt;br /&gt;
          &#039;&amp;lt;li&amp;gt;617-715-4533&amp;lt;/li&amp;gt;&#039; +&lt;br /&gt;
        &#039;&amp;lt;/ul&amp;gt;&#039; +&lt;br /&gt;
      &#039;&amp;lt;/div&amp;gt;&#039; +&lt;br /&gt;
      &#039;&amp;lt;div&amp;gt;&#039; +&lt;br /&gt;
        &#039;&amp;lt;h4&amp;gt;Services&amp;lt;/h4&amp;gt;&#039; +&lt;br /&gt;
        &#039;&amp;lt;ul&amp;gt;&#039; +&lt;br /&gt;
          &#039;&amp;lt;li&amp;gt;&amp;lt;a href=&amp;quot;&#039; + u( &#039;BioMicroCenter:Sequencing&#039; ) + &#039;&amp;quot;&amp;gt;Bulk Sequencing&amp;lt;/a&amp;gt;&amp;lt;/li&amp;gt;&#039; +&lt;br /&gt;
          &#039;&amp;lt;li&amp;gt;&amp;lt;a href=&amp;quot;&#039; + u( &#039;BioMicroCenter:SingleCell&#039; ) + &#039;&amp;quot;&amp;gt;Single Cell&amp;lt;/a&amp;gt;&amp;lt;/li&amp;gt;&#039; +&lt;br /&gt;
          &#039;&amp;lt;li&amp;gt;&amp;lt;a href=&amp;quot;&#039; + u( &#039;BioMicroCenter:SpTx&#039; ) + &#039;&amp;quot;&amp;gt;Spatial Genomics&amp;lt;/a&amp;gt;&amp;lt;/li&amp;gt;&#039; +&lt;br /&gt;
          &#039;&amp;lt;li&amp;gt;&amp;lt;a href=&amp;quot;https://igb.mit.edu/&amp;quot; target=&amp;quot;_blank&amp;quot;&amp;gt;Informatics&amp;lt;/a&amp;gt;&amp;lt;/li&amp;gt;&#039; +&lt;br /&gt;
        &#039;&amp;lt;/ul&amp;gt;&#039; +&lt;br /&gt;
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        &#039;&amp;lt;h4&amp;gt;Resources&amp;lt;/h4&amp;gt;&#039; +&lt;br /&gt;
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          &#039;&amp;lt;li&amp;gt;&amp;lt;a href=&amp;quot;&#039; + u( &#039;BioMicroCenter:Pricing&#039; ) + &#039;&amp;quot;&amp;gt;Grant Support &amp;amp;amp; Pricing&amp;lt;/a&amp;gt;&amp;lt;/li&amp;gt;&#039; +&lt;br /&gt;
          &#039;&amp;lt;li&amp;gt;&amp;lt;a href=&amp;quot;&#039; + u( &#039;BioMicroCenter:Acknowledgement&#039; ) + &#039;&amp;quot;&amp;gt;Acknowledgements&amp;lt;/a&amp;gt;&amp;lt;/li&amp;gt;&#039; +&lt;br /&gt;
        &#039;&amp;lt;/ul&amp;gt;&#039; +&lt;br /&gt;
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&lt;br /&gt;
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      &#039;&amp;lt;span&amp;gt;&#039; +&lt;br /&gt;
        &#039;&amp;lt;a href=&amp;quot;&#039; + u( &#039;Special:UserLogin&#039; ) + &#039;&amp;quot;&amp;gt;Log in&amp;lt;/a&amp;gt;&#039; +&lt;br /&gt;
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        &#039;&amp;lt;a href=&amp;quot;https://accessibility.mit.edu&amp;quot; target=&amp;quot;_blank&amp;quot;&amp;gt;Accessibility&amp;lt;/a&amp;gt;&#039; +&lt;br /&gt;
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&lt;br /&gt;
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&lt;br /&gt;
  /* ── Smooth scroll for anchor links ───────────────────────── */&lt;br /&gt;
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&lt;br /&gt;
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&lt;br /&gt;
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              &#039;position:absolute;left:0;right:0;&#039; +&lt;br /&gt;
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&lt;br /&gt;
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&lt;br /&gt;
  /* ── Main: build and inject the shell ─────────────────────── */&lt;br /&gt;
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&lt;br /&gt;
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&lt;br /&gt;
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&lt;br /&gt;
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      document.querySelector( &#039;.mw-first-heading&#039; );&lt;br /&gt;
    var titleText = titleEl&lt;br /&gt;
      ? titleEl.textContent.trim()&lt;br /&gt;
      : ( mw.config.get( &#039;wgTitle&#039; ) || &#039;BioMicro Center&#039; );&lt;br /&gt;
&lt;br /&gt;
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&lt;br /&gt;
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&lt;br /&gt;
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    layout.className = &#039;bmc-page-layout&#039;;&lt;br /&gt;
&lt;br /&gt;
    /* Sidebar — skip on the home page */&lt;br /&gt;
    var isHome = ( currentPage === &#039;BioMicroCenter&#039; ||&lt;br /&gt;
                   currentPage === &#039;Main_Page&#039; );&lt;br /&gt;
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      layout.appendChild( sidebar );&lt;br /&gt;
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      /* No TOC — collapse to single-column via inline style */&lt;br /&gt;
      layout.style.gridTemplateColumns = &#039;1fr&#039;;&lt;br /&gt;
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&lt;br /&gt;
    /* Main content area */&lt;br /&gt;
    var main = document.createElement( &#039;main&#039; );&lt;br /&gt;
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&lt;br /&gt;
    var footer = buildFooter();&lt;br /&gt;
&lt;br /&gt;
    /* 5. Build wrapper */&lt;br /&gt;
    var wrapper = document.createElement( &#039;div&#039; );&lt;br /&gt;
    wrapper.id = &#039;bmc-wrapper&#039;;&lt;br /&gt;
    wrapper.appendChild( topbar );&lt;br /&gt;
    wrapper.appendChild( header );&lt;br /&gt;
    wrapper.appendChild( hero );&lt;br /&gt;
    wrapper.appendChild( layout );&lt;br /&gt;
    wrapper.appendChild( footer );&lt;br /&gt;
&lt;br /&gt;
    /* 6. Inject into body (prepend so it appears first) */&lt;br /&gt;
    document.body.insertBefore( wrapper, document.body.firstChild );&lt;br /&gt;
&lt;br /&gt;
    /* 7. Add class immediately so the CSS rule kicks in and hides&lt;br /&gt;
          all original MW elements (works for any skin version) */&lt;br /&gt;
    document.body.classList.add( &#039;bmc-active&#039; );&lt;br /&gt;
&lt;br /&gt;
    /* 8. Smooth scroll */&lt;br /&gt;
    initSmoothScroll( main );&lt;br /&gt;
    initSmoothScroll( sidebar || main );&lt;br /&gt;
&lt;br /&gt;
    /* 9. Auto-style plain tables in content */&lt;br /&gt;
    main.querySelectorAll(&lt;br /&gt;
      &#039;.mw-parser-output table:not(.wikitable):not(.infobox):not(.navbox)&#039;&lt;br /&gt;
    ).forEach( function ( t ) {&lt;br /&gt;
      t.classList.add( &#039;wikitable&#039; );&lt;br /&gt;
    } );&lt;br /&gt;
&lt;br /&gt;
    /* 10. Fix row separators on rowspan tables */&lt;br /&gt;
    requestAnimationFrame( function () {&lt;br /&gt;
      fixRowspanSeparators( main );&lt;br /&gt;
    } );&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
  }&lt;br /&gt;
&lt;br /&gt;
  /* ── Entry point ───────────────────────────────────────────── */&lt;br /&gt;
  mw.hook( &#039;wikipage.content&#039; ).add( function () {&lt;br /&gt;
    /* Skip special pages (edit forms, history, etc.) to not break them */&lt;br /&gt;
    var ns     = mw.config.get( &#039;wgNamespaceNumber&#039; );&lt;br /&gt;
    var action = mw.config.get( &#039;wgAction&#039; );&lt;br /&gt;
    if ( action !== &#039;view&#039; ) return;&lt;br /&gt;
    if ( ns &amp;lt; 0 ) return; /* Special: pages */&lt;br /&gt;
&lt;br /&gt;
    var nav = extractNavFromDom();&lt;br /&gt;
    buildShell( nav );&lt;br /&gt;
  } );&lt;br /&gt;
&lt;br /&gt;
} )();&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=User:Asoberan/common.css&amp;diff=131550</id>
		<title>User:Asoberan/common.css</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=User:Asoberan/common.css&amp;diff=131550"/>
		<updated>2026-06-01T15:53:42Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: Created page with &amp;quot;/* ============================================================    BioMicro Center Wiki — CSS-only modern UI    Paste into: User:USERNAME/common.css    Targets: MediaWiki Vector (legacy) skin    Mimics: about.html design without requiring JavaScript    ============================================================ */  /* ── Base &amp;amp; Reset ────────────────────────────────────────────...&amp;quot;&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;/* ============================================================&lt;br /&gt;
   BioMicro Center Wiki — CSS-only modern UI&lt;br /&gt;
   Paste into: User:USERNAME/common.css&lt;br /&gt;
   Targets: MediaWiki Vector (legacy) skin&lt;br /&gt;
   Mimics: about.html design without requiring JavaScript&lt;br /&gt;
   ============================================================ */&lt;br /&gt;
&lt;br /&gt;
/* ── Base &amp;amp; Reset ───────────────────────────────────────────── */&lt;br /&gt;
*, *::before, *::after { box-sizing: border-box; }&lt;br /&gt;
&lt;br /&gt;
/* Universal sans-serif — covers every element MW might leave in a serif/system font */&lt;br /&gt;
body.bmc-active, body.bmc-active * { font-family: &#039;Helvetica Neue&#039;, Arial, sans-serif !important; }&lt;br /&gt;
body.bmc-active code, body.bmc-active pre, body.bmc-active kbd,&lt;br /&gt;
body.bmc-active tt, body.bmc-active samp { font-family: &amp;quot;SFMono-Regular&amp;quot;, Consolas, &amp;quot;Liberation Mono&amp;quot;, Menlo, monospace !important; }&lt;br /&gt;
&lt;br /&gt;
body, html {&lt;br /&gt;
  font-family: &#039;Helvetica Neue&#039;, Arial, sans-serif !important;&lt;br /&gt;
  color: #1a1a1a !important;&lt;br /&gt;
  background: #f4f4f4 !important;&lt;br /&gt;
  line-height: 1.6 !important;&lt;br /&gt;
  font-size: 16px !important;&lt;br /&gt;
  margin: 0 !important;&lt;br /&gt;
  padding: 0 !important;&lt;br /&gt;
}&lt;br /&gt;
&lt;br /&gt;
a { color: #750014 !important; text-decoration: none !important; }&lt;br /&gt;
a:hover { text-decoration: underline !important; color: #5c0010 !important; }&lt;br /&gt;
&lt;br /&gt;
/* ── Contact topbar (CSS pseudo-element, decorative) ─────────── */&lt;br /&gt;
/* Shows contact info as a red strip above the header */&lt;br /&gt;
#mw-page-base {&lt;br /&gt;
  background: #750014 !important;&lt;br /&gt;
  height: 32px !important;&lt;br /&gt;
  position: fixed !important;&lt;br /&gt;
  top: 0 !important;&lt;br /&gt;
  left: 0 !important;&lt;br /&gt;
  right: 0 !important;&lt;br /&gt;
  z-index: 200 !important;&lt;br /&gt;
}&lt;br /&gt;
&lt;br /&gt;
#mw-page-base::after {&lt;br /&gt;
  content: &amp;quot;biomicro@mit.edu  ·  617-715-4533  ·  Building 68-322&amp;quot;;&lt;br /&gt;
  display: block;&lt;br /&gt;
  text-align: right;&lt;br /&gt;
  color: rgba(255,255,255,0.9);&lt;br /&gt;
  font-size: 0.72rem;&lt;br /&gt;
  font-family: &#039;Helvetica Neue&#039;, Arial, sans-serif;&lt;br /&gt;
  padding: 7px 28px;&lt;br /&gt;
  letter-spacing: 0.02em;&lt;br /&gt;
}&lt;br /&gt;
&lt;br /&gt;
/* ── Header (#mw-head) ──────────────────────────────────────── */&lt;br /&gt;
#mw-head {&lt;br /&gt;
  background: #fff !important;&lt;br /&gt;
  border-bottom: 1px solid #e0e0e0 !important;&lt;br /&gt;
  box-shadow: 0 1px 4px rgba(0,0,0,0.07) !important;&lt;br /&gt;
  height: 60px !important;&lt;br /&gt;
  top: 32px !important; /* sit below the red topbar */&lt;br /&gt;
  position: fixed !important;&lt;br /&gt;
  left: 0 !important;&lt;br /&gt;
  right: 0 !important;&lt;br /&gt;
  z-index: 150 !important;&lt;br /&gt;
}&lt;br /&gt;
&lt;br /&gt;
#mw-head-base {&lt;br /&gt;
  height: 92px !important; /* 32px topbar + 60px header */&lt;br /&gt;
  background: #fff !important;&lt;br /&gt;
}&lt;br /&gt;
&lt;br /&gt;
/* Page tabs (Read / Edit / History) */&lt;br /&gt;
#left-navigation {&lt;br /&gt;
  margin-left: 0 !important;&lt;br /&gt;
}&lt;br /&gt;
#left-navigation li a,&lt;br /&gt;
#right-navigation li a {&lt;br /&gt;
  font-family: &#039;Helvetica Neue&#039;, Arial, sans-serif !important;&lt;br /&gt;
  font-size: 0.83rem !important;&lt;br /&gt;
  font-weight: 500 !important;&lt;br /&gt;
  color: #555 !important;&lt;br /&gt;
  background: transparent !important;&lt;br /&gt;
  border: none !important;&lt;br /&gt;
  border-radius: 4px !important;&lt;br /&gt;
  padding: 6px 12px !important;&lt;br /&gt;
  transition: background 0.15s, color 0.15s !important;&lt;br /&gt;
}&lt;br /&gt;
#left-navigation li.selected a,&lt;br /&gt;
#left-navigation li a:hover,&lt;br /&gt;
#right-navigation li a:hover {&lt;br /&gt;
  background: #f5f5f5 !important;&lt;br /&gt;
  color: #750014 !important;&lt;br /&gt;
  border: none !important;&lt;br /&gt;
}&lt;br /&gt;
&lt;br /&gt;
/* Search box in header */&lt;br /&gt;
#p-search {&lt;br /&gt;
  margin-top: 14px !important;&lt;br /&gt;
}&lt;br /&gt;
#searchInput {&lt;br /&gt;
  border: 1px solid #e0e0e0 !important;&lt;br /&gt;
  border-radius: 4px !important;&lt;br /&gt;
  padding: 5px 10px !important;&lt;br /&gt;
  font-size: 0.83rem !important;&lt;br /&gt;
  font-family: &#039;Helvetica Neue&#039;, Arial, sans-serif !important;&lt;br /&gt;
  color: #333 !important;&lt;br /&gt;
  width: 180px !important;&lt;br /&gt;
  outline: none !important;&lt;br /&gt;
  transition: border-color 0.15s !important;&lt;br /&gt;
  background: #fff !important;&lt;br /&gt;
}&lt;br /&gt;
#searchInput:focus { border-color: #750014 !important; }&lt;br /&gt;
#searchButton, #mw-searchButton {&lt;br /&gt;
  background: #750014 !important;&lt;br /&gt;
  border: none !important;&lt;br /&gt;
  border-radius: 4px !important;&lt;br /&gt;
  color: #fff !important;&lt;br /&gt;
  font-size: 0.83rem !important;&lt;br /&gt;
  padding: 5px 10px !important;&lt;br /&gt;
  cursor: pointer !important;&lt;br /&gt;
  margin-left: 4px !important;&lt;br /&gt;
}&lt;br /&gt;
&lt;br /&gt;
/* Personal tools (login/logout) */&lt;br /&gt;
#p-personal li a {&lt;br /&gt;
  font-size: 0.8rem !important;&lt;br /&gt;
  color: #666 !important;&lt;br /&gt;
  font-family: &#039;Helvetica Neue&#039;, Arial, sans-serif !important;&lt;br /&gt;
}&lt;br /&gt;
#p-personal li a:hover { color: #750014 !important; }&lt;br /&gt;
&lt;br /&gt;
/* ── Logo &amp;amp; Sidebar container (#mw-navigation) ──────────────── */&lt;br /&gt;
#mw-navigation {&lt;br /&gt;
  background: #fff !important;&lt;br /&gt;
  border-right: 1px solid #e8e8e8 !important;&lt;br /&gt;
  top: 92px !important; /* below topbar + header */&lt;br /&gt;
  position: fixed !important;&lt;br /&gt;
  left: 0 !important;&lt;br /&gt;
  bottom: 0 !important;&lt;br /&gt;
  width: 180px !important;&lt;br /&gt;
  overflow-y: auto !important;&lt;br /&gt;
  z-index: 100 !important;&lt;br /&gt;
}&lt;br /&gt;
&lt;br /&gt;
/* Logo area */&lt;br /&gt;
#p-logo {&lt;br /&gt;
  position: static !important;&lt;br /&gt;
  width: auto !important;&lt;br /&gt;
  height: auto !important;&lt;br /&gt;
  margin: 0 !important;&lt;br /&gt;
  padding: 20px 16px 16px !important;&lt;br /&gt;
  border-bottom: 1px solid #e8e8e8 !important;&lt;br /&gt;
}&lt;br /&gt;
&lt;br /&gt;
/* Hide the actual logo image; replace with CSS text badge */&lt;br /&gt;
#p-logo a {&lt;br /&gt;
  background-image: none !important;&lt;br /&gt;
  display: flex !important;&lt;br /&gt;
  align-items: center !important;&lt;br /&gt;
  gap: 10px !important;&lt;br /&gt;
  text-decoration: none !important;&lt;br /&gt;
  height: auto !important;&lt;br /&gt;
  width: auto !important;&lt;br /&gt;
  float: none !important;&lt;br /&gt;
}&lt;br /&gt;
&lt;br /&gt;
#p-logo a::before {&lt;br /&gt;
  content: &amp;quot;BMC&amp;quot;;&lt;br /&gt;
  display: flex;&lt;br /&gt;
  align-items: center;&lt;br /&gt;
  justify-content: center;&lt;br /&gt;
  flex-shrink: 0;&lt;br /&gt;
  width: 38px;&lt;br /&gt;
  height: 38px;&lt;br /&gt;
  background: #750014;&lt;br /&gt;
  color: #fff;&lt;br /&gt;
  font-weight: 700;&lt;br /&gt;
  font-size: 0.9rem;&lt;br /&gt;
  border-radius: 4px;&lt;br /&gt;
  letter-spacing: -0.5px;&lt;br /&gt;
  font-family: &#039;Helvetica Neue&#039;, Arial, sans-serif;&lt;br /&gt;
}&lt;br /&gt;
&lt;br /&gt;
#p-logo a::after {&lt;br /&gt;
  content: &amp;quot;MIT BioMicro Center&amp;quot;;&lt;br /&gt;
  display: block;&lt;br /&gt;
  font-size: 0.82rem;&lt;br /&gt;
  font-weight: 700;&lt;br /&gt;
  color: #1a1a1a;&lt;br /&gt;
  font-family: &#039;Helvetica Neue&#039;, Arial, sans-serif;&lt;br /&gt;
  line-height: 1.3;&lt;br /&gt;
  letter-spacing: -0.1px;&lt;br /&gt;
}&lt;br /&gt;
&lt;br /&gt;
/* ── Sidebar navigation portlets (#mw-panel) ─────────────────── */&lt;br /&gt;
#mw-panel {&lt;br /&gt;
  position: static !important;&lt;br /&gt;
  width: auto !important;&lt;br /&gt;
  float: none !important;&lt;br /&gt;
  padding: 12px 0 !important;&lt;br /&gt;
  background: transparent !important;&lt;br /&gt;
}&lt;br /&gt;
&lt;br /&gt;
.portlet,&lt;br /&gt;
.mw-portlet {&lt;br /&gt;
  margin: 0 !important;&lt;br /&gt;
  padding: 0 0 8px !important;&lt;br /&gt;
  border: none !important;&lt;br /&gt;
  background: transparent !important;&lt;br /&gt;
}&lt;br /&gt;
&lt;br /&gt;
/* Portlet headings */&lt;br /&gt;
.portlet h3,&lt;br /&gt;
.mw-portlet-heading,&lt;br /&gt;
div.portlet h3 {&lt;br /&gt;
  font-family: &#039;Helvetica Neue&#039;, Arial, sans-serif !important;&lt;br /&gt;
  font-size: 0.68rem !important;&lt;br /&gt;
  font-weight: 700 !important;&lt;br /&gt;
  text-transform: uppercase !important;&lt;br /&gt;
  letter-spacing: 0.08em !important;&lt;br /&gt;
  color: #999 !important;&lt;br /&gt;
  padding: 10px 16px 4px !important;&lt;br /&gt;
  margin: 0 !important;&lt;br /&gt;
  border: none !important;&lt;br /&gt;
  background: none !important;&lt;br /&gt;
}&lt;br /&gt;
&lt;br /&gt;
/* Portlet links */&lt;br /&gt;
.portlet li,&lt;br /&gt;
.mw-portlet-body li {&lt;br /&gt;
  padding: 0 !important;&lt;br /&gt;
  margin: 0 !important;&lt;br /&gt;
  list-style: none !important;&lt;br /&gt;
  border: none !important;&lt;br /&gt;
}&lt;br /&gt;
.portlet li a,&lt;br /&gt;
.mw-portlet-body li a {&lt;br /&gt;
  display: block !important;&lt;br /&gt;
  padding: 5px 16px !important;&lt;br /&gt;
  font-size: 0.82rem !important;&lt;br /&gt;
  font-family: &#039;Helvetica Neue&#039;, Arial, sans-serif !important;&lt;br /&gt;
  color: #444 !important;&lt;br /&gt;
  border-radius: 4px !important;&lt;br /&gt;
  margin: 0 6px !important;&lt;br /&gt;
  transition: background 0.12s, color 0.12s !important;&lt;br /&gt;
  text-decoration: none !important;&lt;br /&gt;
}&lt;br /&gt;
.portlet li a:hover,&lt;br /&gt;
.mw-portlet-body li a:hover {&lt;br /&gt;
  background: #f0e8ea !important;&lt;br /&gt;
  color: #750014 !important;&lt;br /&gt;
  text-decoration: none !important;&lt;br /&gt;
}&lt;br /&gt;
&lt;br /&gt;
/* ── Main content area (#content) ───────────────────────────── */&lt;br /&gt;
#content,&lt;br /&gt;
#mw-content-wrapper {&lt;br /&gt;
  margin-left: 180px !important; /* match sidebar width */&lt;br /&gt;
  margin-top: 92px !important;   /* topbar 32 + header 60 */&lt;br /&gt;
  margin-right: 0 !important;&lt;br /&gt;
  margin-bottom: 0 !important;&lt;br /&gt;
  padding: 0 !important;&lt;br /&gt;
  background: #fff !important;&lt;br /&gt;
  border: none !important;&lt;br /&gt;
  box-shadow: none !important;&lt;br /&gt;
  border-radius: 0 !important;&lt;br /&gt;
  float: none !important;&lt;br /&gt;
  min-height: calc(100vh - 92px) !important;&lt;br /&gt;
}&lt;br /&gt;
&lt;br /&gt;
/* Hide MediaWiki &amp;quot;From bmcwiki...&amp;quot; subtitle */&lt;br /&gt;
#siteSub,&lt;br /&gt;
#contentSub { display: none !important; }&lt;br /&gt;
&lt;br /&gt;
/* ── Page Hero: style #firstHeading as the about.html hero ───── */&lt;br /&gt;
#firstHeading,&lt;br /&gt;
.mw-first-heading {&lt;br /&gt;
  background: linear-gradient(135deg, #750014 0%, #5c0010 100%) !important;&lt;br /&gt;
  color: #fff !important;&lt;br /&gt;
  font-family: &#039;Helvetica Neue&#039;, Arial, sans-serif !important;&lt;br /&gt;
  font-size: 1.9rem !important;&lt;br /&gt;
  font-weight: 700 !important;&lt;br /&gt;
  letter-spacing: -0.5px !important;&lt;br /&gt;
  padding: 36px 40px 32px !important;&lt;br /&gt;
  margin: 0 !important;&lt;br /&gt;
  border: none !important;&lt;br /&gt;
  width: 100% !important;&lt;br /&gt;
  display: block !important;&lt;br /&gt;
}&lt;br /&gt;
&lt;br /&gt;
/* Breadcrumb &amp;quot;Home ›&amp;quot; before the title */&lt;br /&gt;
#firstHeading::before {&lt;br /&gt;
  content: &amp;quot;Home  ›&amp;quot;;&lt;br /&gt;
  display: block;&lt;br /&gt;
  font-size: 0.78rem;&lt;br /&gt;
  font-weight: 400;&lt;br /&gt;
  color: rgba(255,255,255,0.75);&lt;br /&gt;
  margin-bottom: 8px;&lt;br /&gt;
  letter-spacing: 0;&lt;br /&gt;
}&lt;br /&gt;
&lt;br /&gt;
/* ── Body content area ──────────────────────────────────────── */&lt;br /&gt;
#bodyContent {&lt;br /&gt;
  padding: 36px 40px 60px !important;&lt;br /&gt;
  max-width: 860px !important;&lt;br /&gt;
  background: #fff !important;&lt;br /&gt;
}&lt;br /&gt;
&lt;br /&gt;
/* ── Typography ─────────────────────────────────────────────── */&lt;br /&gt;
.mw-parser-output {&lt;br /&gt;
  font-family: &#039;Helvetica Neue&#039;, Arial, sans-serif !important;&lt;br /&gt;
  font-size: 0.9rem !important;&lt;br /&gt;
  line-height: 1.6 !important;&lt;br /&gt;
  color: #333 !important;&lt;br /&gt;
}&lt;br /&gt;
&lt;br /&gt;
.mw-parser-output h2 {&lt;br /&gt;
  font-size: 1.35rem !important;&lt;br /&gt;
  font-weight: 700 !important;&lt;br /&gt;
  color: #1a1a1a !important;&lt;br /&gt;
  padding-bottom: 10px !important;&lt;br /&gt;
  border-bottom: 2px solid #750014 !important;&lt;br /&gt;
  border-left: none !important;&lt;br /&gt;
  border-top: none !important;&lt;br /&gt;
  border-right: none !important;&lt;br /&gt;
  background: none !important;&lt;br /&gt;
  box-shadow: none !important;&lt;br /&gt;
  border-radius: 0 !important;&lt;br /&gt;
  margin: 40px 0 16px !important;&lt;br /&gt;
  letter-spacing: -0.2px !important;&lt;br /&gt;
  scroll-margin-top: 100px !important;&lt;br /&gt;
}&lt;br /&gt;
.mw-parser-output h2:first-child { margin-top: 0 !important; }&lt;br /&gt;
&lt;br /&gt;
.mw-parser-output h3 {&lt;br /&gt;
  font-size: 1rem !important;&lt;br /&gt;
  font-weight: 600 !important;&lt;br /&gt;
  color: #1a1a1a !important;&lt;br /&gt;
  margin: 24px 0 8px !important;&lt;br /&gt;
}&lt;br /&gt;
&lt;br /&gt;
.mw-parser-output h4 {&lt;br /&gt;
  font-size: 0.88rem !important;&lt;br /&gt;
  font-weight: 700 !important;&lt;br /&gt;
  color: #1a1a1a !important;&lt;br /&gt;
  margin: 16px 0 6px !important;&lt;br /&gt;
  letter-spacing: -0.1px !important;&lt;br /&gt;
}&lt;br /&gt;
&lt;br /&gt;
.mw-parser-output p {&lt;br /&gt;
  margin-bottom: 14px !important;&lt;br /&gt;
  color: #333 !important;&lt;br /&gt;
}&lt;br /&gt;
&lt;br /&gt;
.mw-parser-output ul,&lt;br /&gt;
.mw-parser-output ol {&lt;br /&gt;
  margin: 0 0 14px 22px !important;&lt;br /&gt;
  color: #333 !important;&lt;br /&gt;
}&lt;br /&gt;
.mw-parser-output li { margin-bottom: 4px !important; }&lt;br /&gt;
&lt;br /&gt;
/* ── TOC (Table of Contents) ────────────────────────────────── */&lt;br /&gt;
#toc, .toc {&lt;br /&gt;
  background: #f9f9f9 !important;&lt;br /&gt;
  border: 1px solid #e8e8e8 !important;&lt;br /&gt;
  border-radius: 8px !important;&lt;br /&gt;
  padding: 18px 20px !important;&lt;br /&gt;
  display: inline-block !important;&lt;br /&gt;
  min-width: 200px !important;&lt;br /&gt;
  font-size: 0.82rem !important;&lt;br /&gt;
  float: none !important;&lt;br /&gt;
  margin: 0 0 28px !important;&lt;br /&gt;
}&lt;br /&gt;
.toctitle h2,&lt;br /&gt;
#toc .toctitle h2 {&lt;br /&gt;
  font-size: 0.78rem !important;&lt;br /&gt;
  font-weight: 700 !important;&lt;br /&gt;
  text-transform: uppercase !important;&lt;br /&gt;
  letter-spacing: 0.08em !important;&lt;br /&gt;
  color: #999 !important;&lt;br /&gt;
  margin-bottom: 10px !important;&lt;br /&gt;
  border: none !important;&lt;br /&gt;
  background: none !important;&lt;br /&gt;
  padding: 0 !important;&lt;br /&gt;
  box-shadow: none !important;&lt;br /&gt;
}&lt;br /&gt;
#toc ul, .toc ul {&lt;br /&gt;
  list-style: none !important;&lt;br /&gt;
  margin: 0 !important;&lt;br /&gt;
  padding: 0 !important;&lt;br /&gt;
}&lt;br /&gt;
#toc ul li, .toc ul li { margin: 0 0 2px !important; }&lt;br /&gt;
#toc ul li a, .toc ul li a {&lt;br /&gt;
  font-size: 0.82rem !important;&lt;br /&gt;
  color: #444 !important;&lt;br /&gt;
  padding: 3px 8px !important;&lt;br /&gt;
  display: block !important;&lt;br /&gt;
  border-radius: 4px !important;&lt;br /&gt;
  text-decoration: none !important;&lt;br /&gt;
  transition: background 0.12s, color 0.12s !important;&lt;br /&gt;
}&lt;br /&gt;
#toc ul li a:hover, .toc ul li a:hover {&lt;br /&gt;
  background: #f0e8ea !important;&lt;br /&gt;
  color: #750014 !important;&lt;br /&gt;
  text-decoration: none !important;&lt;br /&gt;
}&lt;br /&gt;
/* Sub-items */&lt;br /&gt;
#toc ul ul li a, .toc ul ul li a {&lt;br /&gt;
  padding-left: 18px !important;&lt;br /&gt;
  font-size: 0.78rem !important;&lt;br /&gt;
  color: #666 !important;&lt;br /&gt;
}&lt;br /&gt;
&lt;br /&gt;
/* ── Edit section links ──────────────────────────────────────── */&lt;br /&gt;
.mw-editsection { display: inline !important; }&lt;br /&gt;
.mw-editsection a {&lt;br /&gt;
  font-size: 0.72rem !important;&lt;br /&gt;
  color: #999 !important;&lt;br /&gt;
  margin-left: 8px !important;&lt;br /&gt;
}&lt;br /&gt;
.mw-editsection a:hover { color: #750014 !important; }&lt;br /&gt;
.mw-editsection-bracket { color: #ddd !important; }&lt;br /&gt;
&lt;br /&gt;
/* ── Tables ─────────────────────────────────────────────────── */&lt;br /&gt;
/* Scoped to .wikitable only — layout tables (no class) handled separately below */&lt;br /&gt;
&lt;br /&gt;
.wikitable {&lt;br /&gt;
  border-collapse: collapse !important;&lt;br /&gt;
  width: 100% !important;&lt;br /&gt;
  margin: 1em 0 !important;&lt;br /&gt;
  font-size: 0.875rem !important;&lt;br /&gt;
  font-family: -apple-system, BlinkMacSystemFont, &#039;Segoe UI&#039;, Helvetica, Arial, sans-serif !important;&lt;br /&gt;
  border: none !important;&lt;br /&gt;
  border-radius: 6px !important;&lt;br /&gt;
  overflow: hidden !important;&lt;br /&gt;
  background: #fff !important;&lt;br /&gt;
  table-layout: auto !important;&lt;br /&gt;
}&lt;br /&gt;
.wikitable th {&lt;br /&gt;
  background: #750014 !important;&lt;br /&gt;
  color: #fff !important;&lt;br /&gt;
  font-weight: 600 !important;&lt;br /&gt;
  font-size: 0.875rem !important;&lt;br /&gt;
  padding: 13px 18px !important;&lt;br /&gt;
  text-align: left !important;&lt;br /&gt;
  border: none !important;&lt;br /&gt;
  white-space: normal !important;&lt;br /&gt;
  overflow-wrap: break-word !important;&lt;br /&gt;
  line-height: 1.4 !important;&lt;br /&gt;
}&lt;br /&gt;
.wikitable td {&lt;br /&gt;
  padding: 12px 18px !important;&lt;br /&gt;
  border: none !important;&lt;br /&gt;
  border-bottom: 1px solid #e0e0e0 !important;&lt;br /&gt;
  vertical-align: top !important;&lt;br /&gt;
  color: #222 !important;&lt;br /&gt;
  font-size: 0.875rem !important;&lt;br /&gt;
  text-align: left !important;&lt;br /&gt;
  line-height: 1.5 !important;&lt;br /&gt;
  background: #fff !important;&lt;br /&gt;
  overflow-wrap: break-word !important;&lt;br /&gt;
}&lt;br /&gt;
/* Row separator */&lt;br /&gt;
.wikitable tr:last-child td { border-bottom: none !important; }&lt;br /&gt;
/* Section-description rows (colspan td used as sub-headers in Pricing tables) */&lt;br /&gt;
.wikitable td[colspan] {&lt;br /&gt;
  background: #f5f5f5 !important;&lt;br /&gt;
  color: #444 !important;&lt;br /&gt;
  font-size: 0.82rem !important;&lt;br /&gt;
  text-align: left !important;&lt;br /&gt;
  line-height: 1.5 !important;&lt;br /&gt;
}&lt;br /&gt;
/* Links inside table cells */&lt;br /&gt;
.wikitable td a { color: #750014 !important; }&lt;br /&gt;
.wikitable td a:hover { color: #750014 !important; text-decoration: underline !important; }&lt;br /&gt;
&lt;br /&gt;
/* Images inside table cells */&lt;br /&gt;
.wikitable td img {&lt;br /&gt;
  max-width: 160px !important;&lt;br /&gt;
  height: auto !important;&lt;br /&gt;
  display: block !important;&lt;br /&gt;
  margin: 6px 0 8px !important;&lt;br /&gt;
}&lt;br /&gt;
&lt;br /&gt;
/* Paragraphs inside table cells */&lt;br /&gt;
.wikitable td p {&lt;br /&gt;
  margin: 0 0 6px !important;&lt;br /&gt;
  font-size: 0.875rem !important;&lt;br /&gt;
  line-height: 1.5 !important;&lt;br /&gt;
  color: #222 !important;&lt;br /&gt;
}&lt;br /&gt;
.wikitable td p:last-child { margin-bottom: 0 !important; }&lt;br /&gt;
&lt;br /&gt;
/* ── Code ───────────────────────────────────────────────────── */&lt;br /&gt;
.mw-parser-output code,&lt;br /&gt;
.mw-parser-output pre,&lt;br /&gt;
.mw-parser-output tt {&lt;br /&gt;
  font-family: &amp;quot;SFMono-Regular&amp;quot;, Consolas, &amp;quot;Liberation Mono&amp;quot;, Menlo, monospace !important;&lt;br /&gt;
  background: #f4f4f4 !important;&lt;br /&gt;
  border: 1px solid #e8e8e8 !important;&lt;br /&gt;
  border-radius: 4px !important;&lt;br /&gt;
  font-size: 0.85rem !important;&lt;br /&gt;
}&lt;br /&gt;
.mw-parser-output code,&lt;br /&gt;
.mw-parser-output tt { padding: 1px 5px !important; }&lt;br /&gt;
.mw-parser-output pre { padding: 12px 16px !important; overflow-x: auto !important; }&lt;br /&gt;
&lt;br /&gt;
/* ── Component classes (wiki editors add these to markup) ────── */&lt;br /&gt;
&lt;br /&gt;
.service-grid {&lt;br /&gt;
  display: grid;&lt;br /&gt;
  grid-template-columns: repeat(2, 1fr);&lt;br /&gt;
  gap: 16px;&lt;br /&gt;
  margin: 20px 0 28px;&lt;br /&gt;
}&lt;br /&gt;
.service-card {&lt;br /&gt;
  background: #f9f9f9;&lt;br /&gt;
  border: 1px solid #e8e8e8;&lt;br /&gt;
  border-radius: 8px;&lt;br /&gt;
  padding: 20px 22px;&lt;br /&gt;
  transition: border-color 0.15s, box-shadow 0.15s;&lt;br /&gt;
}&lt;br /&gt;
.service-card:hover { border-color: #c8a0a8; box-shadow: 0 2px 10px rgba(117,0,20,0.07); }&lt;br /&gt;
.service-card h4 { font-size: 0.88rem !important; font-weight: 700 !important; color: #1a1a1a !important; margin-bottom: 6px !important; }&lt;br /&gt;
.service-card p { font-size: 0.82rem !important; color: #555 !important; margin: 0 !important; line-height: 1.5 !important; }&lt;br /&gt;
&lt;br /&gt;
.staff-grid { display: grid; grid-template-columns: repeat(3, 1fr); gap: 14px; }&lt;br /&gt;
.staff-card { background: #f9f9f9; border: 1px solid #e8e8e8; border-radius: 8px; padding: 16px 18px; }&lt;br /&gt;
.staff-card .staff-name { font-size: 0.88rem; font-weight: 700; color: #1a1a1a; display: block; }&lt;br /&gt;
.staff-card .staff-role { font-size: 0.78rem; color: #666; margin-top: 3px; line-height: 1.4; display: block; }&lt;br /&gt;
.staff-card.director { grid-column: 1 / -1; background: #fdf5f6; border-color: #d4a0a8; display: flex; align-items: center; gap: 18px; }&lt;br /&gt;
.director-badge { width: 44px; height: 44px; background: #750014; border-radius: 50%; display: flex; align-items: center; justify-content: center; color: #fff; font-weight: 700; font-size: 1rem; flex-shrink: 0; }&lt;br /&gt;
.staff-card.director .staff-name { font-size: 1rem !important; }&lt;br /&gt;
.staff-card.director .staff-role { font-size: 0.82rem !important; color: #444 !important; font-weight: 500; }&lt;br /&gt;
&lt;br /&gt;
.pub-list { list-style: none !important; margin: 0 !important; padding: 0 !important; }&lt;br /&gt;
.pub-list li { padding: 14px 0 !important; border-bottom: 1px solid #f0f0f0 !important; font-size: 0.85rem !important; }&lt;br /&gt;
.pub-list li:last-child { border-bottom: none !important; }&lt;br /&gt;
.pub-list .pub-lab { font-weight: 700; color: #1a1a1a; display: block; }&lt;br /&gt;
.pub-list .pub-desc { color: #444; margin-top: 3px; line-height: 1.5; display: block; }&lt;br /&gt;
.pub-list .pub-cite { display: inline-block; margin-top: 5px; font-size: 0.75rem; background: #f0f0f0; color: #555; padding: 2px 8px; border-radius: 10px; }&lt;br /&gt;
&lt;br /&gt;
.affil-grid { display: grid; grid-template-columns: repeat(2, 1fr); gap: 14px; margin: 18px 0 24px; }&lt;br /&gt;
.affil-card { background: #f9f9f9; border: 1px solid #e8e8e8; border-radius: 8px; padding: 16px 18px; }&lt;br /&gt;
.affil-card .affil-name { font-size: 0.85rem; font-weight: 700; color: #1a1a1a; display: block; }&lt;br /&gt;
.affil-card .affil-rep { font-size: 0.78rem; color: #666; margin-top: 4px; display: block; }&lt;br /&gt;
&lt;br /&gt;
.note { background: #fdf6f0; border-left: 3px solid #e07b39; padding: 10px 14px; border-radius: 0 6px 6px 0; font-size: 0.82rem; color: #555; margin: 12px 0 18px; }&lt;br /&gt;
.note strong { color: #1a1a1a; }&lt;br /&gt;
.note.note-info { background: #f0f6ff; border-left-color: #3b7dd8; }&lt;br /&gt;
&lt;br /&gt;
.contact-block { background: #f9f9f9; border: 1px solid #e8e8e8; border-radius: 8px; padding: 22px 24px; display: flex; gap: 40px; flex-wrap: wrap; font-size: 0.85rem; }&lt;br /&gt;
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.contact-item .label { font-size: 0.72rem; font-weight: 700; text-transform: uppercase; letter-spacing: 0.07em; color: #999; }&lt;br /&gt;
.contact-item .value { color: #1a1a1a; font-weight: 500; }&lt;br /&gt;
.contact-item a { color: #750014 !important; }&lt;br /&gt;
&lt;br /&gt;
/* ── Footer ─────────────────────────────────────────────────── */&lt;br /&gt;
#footer {&lt;br /&gt;
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/* Footer top accent */&lt;br /&gt;
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&lt;br /&gt;
/* ── Responsive ─────────────────────────────────────────────── */&lt;br /&gt;
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&lt;br /&gt;
/* ── Hide original MW chrome once the BMC wrapper is injected ── */&lt;br /&gt;
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/* ── BMC Shell (injected by common.js) ─────────────────────── */&lt;br /&gt;
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.bmc-breadcrumb { font-size: 0.78rem; margin-bottom: 10px; opacity: 0.75; }&lt;br /&gt;
.bmc-breadcrumb a { color: #fff !important; text-decoration: none !important; }&lt;br /&gt;
.bmc-breadcrumb .sep { margin: 0 6px; }&lt;br /&gt;
.bmc-page-hero h1 { font-size: 2rem !important; font-weight: 700 !important; letter-spacing: -0.5px !important; color: #fff !important; margin: 0 !important; border: none !important; background: none !important; padding: 0 !important; box-shadow: none !important; }&lt;br /&gt;
&lt;br /&gt;
/* Page layout */&lt;br /&gt;
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&lt;br /&gt;
/* Sidebar */&lt;br /&gt;
.bmc-sidebar { position: sticky; top: 84px; align-self: start; }&lt;br /&gt;
.bmc-toc { background: #f9f9f9; border: 1px solid #e8e8e8; border-radius: 8px; padding: 20px; }&lt;br /&gt;
.bmc-toc h3 { font-size: 0.78rem !important; font-weight: 700 !important; text-transform: uppercase !important; letter-spacing: 0.08em !important; color: #999 !important; margin-bottom: 12px !important; border: none !important; background: none !important; padding: 0 !important; box-shadow: none !important; }&lt;br /&gt;
.bmc-toc ul { list-style: none !important; margin: 0 !important; padding: 0 !important; }&lt;br /&gt;
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.bmc-toc ul li a { font-size: 0.82rem !important; color: #444 !important; padding: 4px 8px !important; display: block !important; border-radius: 4px !important; text-decoration: none !important; transition: background 0.12s, color 0.12s; }&lt;br /&gt;
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/* Content */&lt;br /&gt;
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.bmc-content h2 { font-size: 1.35rem !important; font-weight: 700 !important; color: #1a1a1a !important; padding-bottom: 10px !important; border-bottom: 2px solid #750014 !important; border-left: none !important; border-top: none !important; border-right: none !important; background: none !important; box-shadow: none !important; border-radius: 0 !important; margin: 48px 0 18px !important; letter-spacing: -0.2px !important; scroll-margin-top: 88px !important; }&lt;br /&gt;
.bmc-content h2:first-child { margin-top: 0 !important; }&lt;br /&gt;
.bmc-content h3 { font-size: 1rem !important; font-weight: 600 !important; color: #1a1a1a !important; margin: 28px 0 10px !important; scroll-margin-top: 88px !important; }&lt;br /&gt;
.bmc-content p { margin-bottom: 14px; font-size: 0.9rem; color: #333; }&lt;br /&gt;
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.bmc-content ul, .bmc-content ol { margin: 0 0 14px 22px; color: #333; }&lt;br /&gt;
.bmc-content li { margin-bottom: 4px; }&lt;br /&gt;
&lt;br /&gt;
/* Footer */&lt;br /&gt;
/* Edit bar — shown to logged-in users at top of content area */&lt;br /&gt;
.bmc-edit-bar { display: flex !important; gap: 12px !important; justify-content: flex-end !important; padding: 6px 0 10px !important; border-bottom: 1px solid #e8e8e8 !important; margin-bottom: 16px !important; }&lt;br /&gt;
.bmc-edit-bar a { font-size: 0.78rem !important; color: #750014 !important; text-decoration: none !important; font-weight: 500 !important; }&lt;br /&gt;
.bmc-edit-bar a:hover { text-decoration: underline !important; }&lt;br /&gt;
&lt;br /&gt;
.bmc-footer { background: #1a1a1a; color: #aaa; padding: 40px 0; font-size: 0.82rem; font-family: &#039;Helvetica Neue&#039;, Arial, sans-serif; }&lt;br /&gt;
.bmc-footer-inner { max-width: 1140px; margin: 0 auto; padding: 0 24px; display: grid; grid-template-columns: 1fr 1fr 1fr; gap: 32px; }&lt;br /&gt;
.bmc-footer h4 { font-size: 0.75rem !important; font-weight: 700 !important; text-transform: uppercase !important; letter-spacing: 0.08em !important; color: #fff !important; margin-bottom: 12px !important; border: none !important; background: none !important; padding: 0 !important; box-shadow: none !important; }&lt;br /&gt;
.bmc-footer ul { list-style: none !important; margin: 0 !important; padding: 0 !important; }&lt;br /&gt;
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.bmc-footer ul li a { color: #aaa !important; text-decoration: none !important; }&lt;br /&gt;
.bmc-footer ul li a:hover { color: #fff !important; text-decoration: none !important; }&lt;br /&gt;
.bmc-footer-bottom { max-width: 1140px; margin: 24px auto 0; padding: 20px 24px 0; border-top: 1px solid #333; display: flex; justify-content: space-between; align-items: center; flex-wrap: wrap; gap: 8px; font-size: 0.77rem; color: #666; }&lt;br /&gt;
.bmc-footer-bottom a { color: #666 !important; }&lt;br /&gt;
.bmc-footer-bottom a:hover { color: #fff !important; }&lt;br /&gt;
&lt;br /&gt;
/* Responsive (bmc shell) */&lt;br /&gt;
@media (max-width: 860px) {&lt;br /&gt;
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}&lt;br /&gt;
&lt;br /&gt;
/* ── Content overrides: clean up main-page legacy styling ─── */&lt;br /&gt;
&lt;br /&gt;
/* Hide the BMC header/logo sticker images (JS handles this by src; CSS is a fallback) */&lt;br /&gt;
img[src*=&amp;quot;BMC_Header&amp;quot;],&lt;br /&gt;
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img[src*=&amp;quot;Bmc_logo&amp;quot;] {&lt;br /&gt;
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/* Keep header background even if inline style is present */&lt;br /&gt;
.wikitable th[style*=&amp;quot;background&amp;quot;] { background: #750014 !important; }&lt;br /&gt;
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/* ── Layout tables (non-wikitable): clean up legacy styling ──── */&lt;br /&gt;
/* The wiki uses plain tables (no .wikitable) for service grids and&lt;br /&gt;
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&lt;br /&gt;
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&lt;br /&gt;
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&lt;br /&gt;
/* Service card headings: wiki uses &amp;lt;big&amp;gt;&amp;lt;b&amp;gt;title&amp;lt;/b&amp;gt;&amp;lt;/big&amp;gt; */&lt;br /&gt;
.bmc-content .mw-parser-output big b,&lt;br /&gt;
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  font-weight: 700 !important;&lt;br /&gt;
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  display: block !important;&lt;br /&gt;
  margin-bottom: 6px !important;&lt;br /&gt;
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}&lt;br /&gt;
&lt;br /&gt;
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.bmc-content .mw-parser-output &amp;gt; table:not(.wikitable) ul,&lt;br /&gt;
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  font-size: 0.82rem !important;&lt;br /&gt;
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}&lt;br /&gt;
&lt;br /&gt;
/* Plain layout cells without border (e.g. News page columns) */&lt;br /&gt;
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  vertical-align: top !important;&lt;br /&gt;
  background: transparent !important;&lt;br /&gt;
}&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:People&amp;diff=131442</id>
		<title>BioMicroCenter:People</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:People&amp;diff=131442"/>
		<updated>2026-05-07T19:21:58Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: Added Uday&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;{{BioMicroCenter}}&lt;br /&gt;
&amp;lt;!-- Tables have been created with pipe syntax to present this information --&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== BioMicro Center Staff ==&lt;br /&gt;
&lt;br /&gt;
{| border=0 align=&amp;quot;center&amp;quot; cellpadding=&amp;quot;5&amp;quot;&lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:SL_07.jpg|right|100px]]&lt;br /&gt;
[[User:Stuart S. Levine|&#039;&#039;&#039;Stuart Levine&#039;&#039;&#039;]] - Director&lt;br /&gt;
* Phone: 617-452-2949&lt;br /&gt;
* Email: slevine@mit.edu&lt;br /&gt;
|}&lt;br /&gt;
&amp;lt;!-- This creates a centered table that is just a single cell 350 wide with a purple border --&amp;gt;&lt;br /&gt;
&lt;br /&gt;
=== WET LAB ===&lt;br /&gt;
{| border=0 align=&amp;quot;center&amp;quot; cellpadding=&amp;quot;5&amp;quot;&lt;br /&gt;
 |- style=&amp;quot;height:150xp;&amp;quot;&lt;br /&gt;
&amp;lt;!-- This centered table has no border and a general height per row of 150xp --&amp;gt;&lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[User:Johan_Gibcus|&#039;&#039;&#039;Johan Gibcus&#039;&#039;&#039;]], Ph.D. - Program Manager&lt;br /&gt;
* Phone: x54533&lt;br /&gt;
* Email: gibcus@mit.edu&lt;br /&gt;
|width=25|&lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:AlexS.jpg|right|100px]]&lt;br /&gt;
[[User:Alexei|&#039;&#039;&#039;Alex Stortchevoi&#039;&#039;&#039;]], Ph.D. - Research Scientist in Biology&lt;br /&gt;
* Phone: x54533&lt;br /&gt;
* Email: alyosha@mit.edu&lt;br /&gt;
|width=25|&lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:NKBMC04082024.jpg|right|100px]]&lt;br /&gt;
[[User:Noelani Kamelamela|&#039;&#039;&#039;Noelani Kamelamela&#039;&#039;&#039;]] - Lab Manager&lt;br /&gt;
* Phone: x54533&lt;br /&gt;
* Email: hilo1@mit.edu&lt;br /&gt;
|- style=&amp;quot;height:25px;&amp;quot;&lt;br /&gt;
 | colspan=&amp;quot;5&amp;quot; |&lt;br /&gt;
 |- style=&amp;quot;height:150xp;&amp;quot;&lt;br /&gt;
&amp;lt;!-- This gives us a &amp;quot;linebreak&amp;quot; of sorts, the colspan indicated is 5 which means there should only be five cells in a row (three bios and two |width=25| cells), anything else will mess up this table formatting, but that can be fixed by resetting the colspan for all &amp;quot;linebreaks&amp;quot; (rowspan can be used similarly) --&amp;gt;&lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Hallee_ChristopherS.jpg|right|100px]]&lt;br /&gt;
[[User:Challee|&#039;&#039;&#039;Christopher Hallee&#039;&#039;&#039;]] - Sequencing Specialist&lt;br /&gt;
* Phone: x54533&lt;br /&gt;
* Email: challee@mit.edu&lt;br /&gt;
Illumina Sequencing and Quality Control Specialist&lt;br /&gt;
|width=25|&lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Avanyish.jpg|right|100px]]&lt;br /&gt;
[[User:Avanyish|&#039;&#039;&#039;Avanyish Toniappa&#039;&#039;&#039;]] - Automation Specialist&lt;br /&gt;
* Phone: x54533&lt;br /&gt;
* Email: toniappa@mit.edu&lt;br /&gt;
Automation and Library Preparation Specialist&lt;br /&gt;
|width=25|&lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Skylarhebert.JPEG|right|100px]]&lt;br /&gt;
[[User:Skylar|&#039;&#039;&#039;Sky Hebert&#039;&#039;&#039;]] - Library Preparation Specialist&lt;br /&gt;
* Phone: x54533&lt;br /&gt;
* Email: shebert@mit.edu&lt;br /&gt;
Library Preparation and ONT Specialist&lt;br /&gt;
&amp;lt;!--end of this line --&amp;gt;&lt;br /&gt;
 |- style=&amp;quot;height:25px;&amp;quot;&lt;br /&gt;
 | colspan=&amp;quot;5&amp;quot; |&lt;br /&gt;
 |- style=&amp;quot;height:150xp;&amp;quot; &lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:cstj.jpg|right|100px]]&lt;br /&gt;
[[User:cstjohn|&#039;&#039;&#039;Caroline St John&#039;&#039;&#039;]] - Co-op&lt;br /&gt;
* Phone: x54533&lt;br /&gt;
* Email: cstjohn@mit.edu&lt;br /&gt;
|width=25|&lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Uday.jpeg|right|100px]]&lt;br /&gt;
Uday Sethi - Co-op&lt;br /&gt;
* Email: udays108@mit.edu&lt;br /&gt;
|}&lt;br /&gt;
&amp;lt;!-- Everything in this table has to be entered before the |}, and now enough has been commented so that anyone can make sense of this --&amp;gt;&lt;br /&gt;
 &lt;br /&gt;
=== [[BioMicroCenter:BioInformaticsStaff|INFORMATICS LAB]] ===&lt;br /&gt;
{| border=0 align=&amp;quot;center&amp;quot; cellpadding=&amp;quot;5&amp;quot;&lt;br /&gt;
 |- style=&amp;quot;height:150xp;&amp;quot;&lt;br /&gt;
&amp;lt;!-- This gives us a &amp;quot;linebreak&amp;quot; of sorts, the colspan indicated is 5 which means there should only be five cells in a row (three bios and two |width=25| cells), anything else will mess up this table formatting, but that can be fixed by resetting the colspan for all &amp;quot;linebreaks&amp;quot; (rowspan can be used similarly) --&amp;gt;&lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:VincentBMC.jpg‎|right|100px]]&lt;br /&gt;
[[User:Vincent L. Butty|&#039;&#039;&#039;Vincent Butty&#039;&#039;&#039;]], M.D., Ph.D. - BioInformatics Research Scientist&lt;br /&gt;
* Phone: x44945&lt;br /&gt;
* Email: vbutty@mit.edu&lt;br /&gt;
 |width=25|&lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Duaduan Ma.jpg‎|right|100px]]&lt;br /&gt;
[[User:Duanduan Ma|&#039;&#039;&#039;Duanduan Ma&#039;&#039;&#039;]], Ph.D. - BioInformatics Research Scientist&lt;br /&gt;
* Phone: x44945&lt;br /&gt;
* Email: duan@mit.edu&lt;br /&gt;
 |width=25|&lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image: yann.jpeg|right|100px]]&lt;br /&gt;
[[User:yanvrb|&#039;&#039;&#039;Yann Vanrobaeys&#039;&#039;&#039;]], Ph.D. - Bioinformatics Research Scientist&lt;br /&gt;
* Phone: x44945&lt;br /&gt;
* Email: yannvrb@mit.edu&lt;br /&gt;
 &lt;br /&gt;
 |- style=&amp;quot;height:25px;&amp;quot;&lt;br /&gt;
 | colspan=&amp;quot;5&amp;quot; |&lt;br /&gt;
 |- style=&amp;quot;height:150xp;&amp;quot;&lt;br /&gt;
 &lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Charlie_Demurjian_headshot.jpg|right|100px]]&lt;br /&gt;
[https://www.linkedin.com/in/cdemurjian/ &#039;&#039;&#039;Charlie Demurjian&#039;&#039;&#039;] - Lead Data Specialist&lt;br /&gt;
* Phone: x44945&lt;br /&gt;
* Email: cdemu@mit.edu&lt;br /&gt;
 &lt;br /&gt;
 |width=25|&lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:fl13.jpeg|right|100px]]&lt;br /&gt;
[[User:taishajo|&#039;&#039;&#039;Taisha Joseph-Risch&#039;&#039;&#039;]] - Research Data Specialist&lt;br /&gt;
* Phone: x44945&lt;br /&gt;
* Email: taishajo@mit.edu&lt;br /&gt;
 &lt;br /&gt;
 |width=25|&lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Huiming_Ding.JPG|right|100px]]&lt;br /&gt;
[[User:Huiming Ding|&#039;&#039;&#039;Huiming Ding&#039;&#039;&#039;]], Ph.D. - Research Computing Scientist&lt;br /&gt;
* Phone: x44945&lt;br /&gt;
* Email: huiming@mit.edu&lt;br /&gt;
&lt;br /&gt;
 |- style=&amp;quot;height:25px;&amp;quot;&lt;br /&gt;
 | colspan=&amp;quot;5&amp;quot; |&lt;br /&gt;
 |- style=&amp;quot;height:150xp;&amp;quot;&lt;br /&gt;
&lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:allen.jpg|right|100px]]&lt;br /&gt;
[[User:Asoberan|&#039;&#039;&#039;Allen Soberanes&#039;&#039;&#039;]] - Systems and IT Administrator&lt;br /&gt;
* Phone: x44945&lt;br /&gt;
* Email: asoberan@mit.edu&lt;br /&gt;
&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
== BioMicro Center Alumni ==&lt;br /&gt;
=== STAFF ===&lt;br /&gt;
{| border=0 align=&amp;quot;center&amp;quot; cellpadding=&amp;quot;5&amp;quot;&lt;br /&gt;
 |- style=&amp;quot;height:150xp;&amp;quot; &lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Pradhan-Dikshant.jpg|right|100px]] &lt;br /&gt;
[[User:Dpradhan|&#039;&#039;&#039;Dikshant Pradhan&#039;&#039;&#039;]]&lt;br /&gt;
* Start Date - End Date &lt;br /&gt;
* Research Specialist&lt;br /&gt;
* Email: dpradhan@mit.edu&lt;br /&gt;
* Currently in graduate school at BU&lt;br /&gt;
 |width=25|&lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:180611_Sam.jpg|right|100px]]&lt;br /&gt;
[[User:Sam Mildrum|&#039;&#039;&#039;Sam Mildrum&#039;&#039;&#039;]]  &lt;br /&gt;
* June 2018 - September 2022 &lt;br /&gt;
* Automation Specialist&lt;br /&gt;
* Email: sam.mildrum@maine.edu&lt;br /&gt;
* Currently Senior Research Associate at Omega Therapeutics&lt;br /&gt;
 |width=25|&lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:P2130493.JPG|right|100px]]&lt;br /&gt;
[[User:Barbara Karampalas|&#039;&#039;&#039;Barbara Karampalas&#039;&#039;&#039;]]&lt;br /&gt;
* Jan 2011 - May 2012&lt;br /&gt;
* Technical Associate&lt;br /&gt;
* Currently Lab Manager for Dr. Chris Voigt&lt;br /&gt;
 |- style=&amp;quot;height:25px;&amp;quot;&lt;br /&gt;
 | colspan=&amp;quot;5&amp;quot; |&lt;br /&gt;
 |- style=&amp;quot;height:150xp;&amp;quot; &lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:kthai.jpg|right|100px]]&lt;br /&gt;
[[User:Kevin K. Thai|&#039;&#039;&#039;Kevin Thai&#039;&#039;&#039;]]&lt;br /&gt;
* Sept 2009 - July 2012&lt;br /&gt;
* Technical Assistant&lt;br /&gt;
* PhD Biology at MIT 2017&lt;br /&gt;
* Currently Scientist at Amgen&lt;br /&gt;
 |width=25|&lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Sinapius.jpg|right|100px]]&lt;br /&gt;
[[User:Ryan Sinapius|&#039;&#039;&#039;Ryan Sinapius&#039;&#039;&#039;]]&lt;br /&gt;
* Nov 2010 - Nov 2012&lt;br /&gt;
* Technical Assistant&lt;br /&gt;
* Currently Research Associate at [http://www.cellsignal.com Cell Signaling Technology]&lt;br /&gt;
 |width=25|&lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Rabo.jpg|right|100px]] &lt;br /&gt;
[[User:Ryan Abo|&#039;&#039;&#039;Ryan Abo&#039;&#039;&#039;]] &lt;br /&gt;
* Apr 2012- Apr 2013&lt;br /&gt;
* BioInformatics Scientist&lt;br /&gt;
* Currently Sr Scientist at Ribon Therapeutics&lt;br /&gt;
&amp;lt;!--end of this line --&amp;gt;&lt;br /&gt;
 |- style=&amp;quot;height:25px;&amp;quot;&lt;br /&gt;
 | colspan=&amp;quot;5&amp;quot; |&lt;br /&gt;
 |- style=&amp;quot;height:150xp;&amp;quot; &lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Scott_Morin.jpg|right|100px]]&lt;br /&gt;
[[User:Scott J. Morin|&#039;&#039;&#039;Scott J. Morin&#039;&#039;&#039;]]&lt;br /&gt;
*July 2012 - June 2014&lt;br /&gt;
*Technical Assistant&lt;br /&gt;
*DO at University of New England College of Osteopathic Medicine 2014&lt;br /&gt;
*Currently Resident Physician at Mt Auburn Hospital&lt;br /&gt;
* Email: smorin89@gmail.com&lt;br /&gt;
&lt;br /&gt;
 |width=25|&lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Shmulik Motola.jpg|right|100px]]&lt;br /&gt;
[[User:Shmulik Motola|&#039;&#039;&#039;Shmulik Motola&#039;&#039;&#039;]] &lt;br /&gt;
* January 2012 - October 2015&lt;br /&gt;
* Lab manager&lt;br /&gt;
* Currently Core Director, G-INCPM at Weizmann Institute &lt;br /&gt;
* Email: shmulik.motola@weizmann.ac.il&lt;br /&gt;
 |width=25|&lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Vidya_S.png|right|100px]]&lt;br /&gt;
[[User:Vidya Subramanian|&#039;&#039;&#039;Vidya Subramanian&#039;&#039;&#039;]]&lt;br /&gt;
* June 2015 - April 2016&lt;br /&gt;
* Research Scientist&lt;br /&gt;
* Currently Research Scientist II at Broad Institute &lt;br /&gt;
* Email: &lt;br /&gt;
 |- style=&amp;quot;height:25px;&amp;quot;&lt;br /&gt;
 | colspan=&amp;quot;5&amp;quot; |&lt;br /&gt;
 |- style=&amp;quot;height:150xp;&amp;quot; &lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:miniterm21.jpg|right|100px]]&lt;br /&gt;
[[User:Leigh J Manley|&#039;&#039;&#039;Leigh Manley&#039;&#039;&#039;]]&lt;br /&gt;
* April 2014 - May 2016&lt;br /&gt;
* Technical Assistant&lt;br /&gt;
* PhD UTSW&lt;br /&gt;
* Email: leigh.j.manley@gmail.com&lt;br /&gt;
 |width=25|&lt;br /&gt;
&amp;lt;!-- This creates an empty cell 25 wide with no border and this is the second cell in the row --&amp;gt;&lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Manlinlab7.jpg|right|100px]]&lt;br /&gt;
[[User:Manlin Luo|&#039;&#039;&#039;Manlin Luo&#039;&#039;&#039;]] &lt;br /&gt;
* 1997-2016&lt;br /&gt;
* Technical Associate&lt;br /&gt;
* Retired&lt;br /&gt;
 |width=25|&lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:MGravina.jpg|right|80px]]&lt;br /&gt;
[[User:Michael Gravina|&#039;&#039;&#039;Michael Gravina&#039;&#039;&#039;]] &lt;br /&gt;
* December 2010 - August 2016&lt;br /&gt;
* Technical Associate&lt;br /&gt;
* Currently Research Associate at OSU&lt;br /&gt;
 |- style=&amp;quot;height:25px;&amp;quot;&lt;br /&gt;
 | colspan=&amp;quot;5&amp;quot; |&lt;br /&gt;
 |- style=&amp;quot;height:150xp;&amp;quot; &lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:jie.jpg‎|right|100px]] &lt;br /&gt;
[[User:Jie Wu|&#039;&#039;&#039;Jie Wu&#039;&#039;&#039;]] &lt;br /&gt;
* BioInformatics Scientist&lt;br /&gt;
* September 2013-November 2016&lt;br /&gt;
* Currently Sr Research Scientist at Phillips&lt;br /&gt;
 |width=25|&lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:IMG_0921.jpg|right|100px]]&lt;br /&gt;
[[User:Fangming Zheng|&#039;&#039;&#039;Fangming Zheng&#039;&#039;&#039;]]&lt;br /&gt;
* Technical Assistant&lt;br /&gt;
*Sample Prep&lt;br /&gt;
*July 2016-June 2017&lt;br /&gt;
 |width=25|&lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Jon Penterman.jpg|right|100px]]&lt;br /&gt;
[[User:Jon Penterman|&#039;&#039;&#039;Jon Penterman&#039;&#039;&#039;]] &lt;br /&gt;
* Lab Manager&lt;br /&gt;
* December 2015-November 2017&lt;br /&gt;
* Currently Scientist at CRISPR Therapeutics&lt;br /&gt;
 |- style=&amp;quot;height:25px;&amp;quot;&lt;br /&gt;
 | colspan=&amp;quot;5&amp;quot; |&lt;br /&gt;
 |- style=&amp;quot;height:150xp;&amp;quot; &lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Index.jpg|right|100px]]&lt;br /&gt;
[[User:Namita Wadke|&#039;&#039;&#039;Namita Wadke&#039;&#039;&#039;]] &lt;br /&gt;
* Technical Assistant: 2016-2018&lt;br /&gt;
* Currently at Aldatu Biosciences&lt;br /&gt;
 |width=25|&lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Vineet3.jpeg|right|125px]]&lt;br /&gt;
[[User:Vineet Vishwanath|&#039;&#039;&#039;Vineet Vishwanath&#039;&#039;&#039;]] &lt;br /&gt;
*Sequencing and Quality Control Specialist&lt;br /&gt;
*August 2018-August 2020&lt;br /&gt;
|width=25|&lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Austinh.jpg|right|100px]]&lt;br /&gt;
[[User:Austin Hendricks|&#039;&#039;&#039;Austin Hendricks&#039;&#039;&#039;]] &lt;br /&gt;
* Coop: 2013&lt;br /&gt;
* Automation Technologist: 2015-2021&lt;br /&gt;
* Currently Grad Student at UNM&lt;br /&gt;
|- style=&amp;quot;height:25px;&amp;quot;&lt;br /&gt;
| colspan=&amp;quot;5&amp;quot; |&lt;br /&gt;
|- style=&amp;quot;height:150xp;&amp;quot; &lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:ChloeM.jpg|right|100px]]&lt;br /&gt;
[[User:Chloe Morgan|&#039;&#039;&#039;Chloe Morgan&#039;&#039;&#039;]] &lt;br /&gt;
* Senior Research Support Associate: 2019-2021&lt;br /&gt;
* MD Tufts University Medical School&lt;br /&gt;
 |width=25|&lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Fugen.jpg|right|100px]]&lt;br /&gt;
[[User:Fugen Li|&#039;&#039;&#039;Fugen Li&#039;&#039;&#039;]]&lt;br /&gt;
* July 2010 - Dec 2011&lt;br /&gt;
* BioInformatics Scientist&lt;br /&gt;
* Currently Sr Scientist at Sanofi-Aventis&lt;br /&gt;
 |width=25|&lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Stephen.JPG|right|100px]] &lt;br /&gt;
[[User:Stephen Goldman|&#039;&#039;&#039;Stephen Goldman&#039;&#039;&#039;]] &lt;br /&gt;
* System Administrator&lt;br /&gt;
* 1962/11/26-2022/09/30&lt;br /&gt;
|- style=&amp;quot;height:25px;&amp;quot;&lt;br /&gt;
| colspan=&amp;quot;5&amp;quot; |&lt;br /&gt;
|- style=&amp;quot;height:150xp;&amp;quot; &lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:ali.jpg|right|100px]]&lt;br /&gt;
[[User:Allison Perrotta|&#039;&#039;&#039;Allison Perrotta&#039;&#039;&#039;]] &lt;br /&gt;
* Nov 2008 - Sep 2010&lt;br /&gt;
* Technical Assistant &lt;br /&gt;
* PhD Biological Engineering at MIT 2017&lt;br /&gt;
* Currently FAS II at Illumina&lt;br /&gt;
|width=25|&lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[User:Jingzhi Zhu|&#039;&#039;&#039;Jingzhi Zhu&#039;&#039;&#039;]] &lt;br /&gt;
* Research Computing Specialist&lt;br /&gt;
* Email: jingzhi@mit.edu&lt;br /&gt;
|width=25|&lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:tatum-2.jpeg|right|100px]]&lt;br /&gt;
[[User:Tmurdock|&#039;&#039;&#039;Tatum Murdock&#039;&#039;&#039;]] &lt;br /&gt;
* Automation Specialist 2021-2024&lt;br /&gt;
* Currently Grad Student at Vanderbilt University&lt;br /&gt;
|- style=&amp;quot;height:25px;&amp;quot;&lt;br /&gt;
| colspan=&amp;quot;5&amp;quot; |&lt;br /&gt;
|- style=&amp;quot;height:150xp;&amp;quot; &lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:SKR_BMC.jpg|right|100px]]&lt;br /&gt;
[[User:skeppler|&#039;&#039;&#039;Sabine Keppler Ross&#039;&#039;&#039;]] &lt;br /&gt;
* Library Preparation 2024-2025&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
=== UROPS / COOPS ===&lt;br /&gt;
&lt;br /&gt;
{| border=0 align=&amp;quot;center&amp;quot; cellpadding=&amp;quot;5&amp;quot;&lt;br /&gt;
 |- style=&amp;quot;height:150xp;&amp;quot; &lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:AvantiShrikumar.jpg|right|100px]]&lt;br /&gt;
[[User:Avanti Shrikumar|&#039;&#039;&#039;Avanti Shrikumar&#039;&#039;&#039;]]&lt;br /&gt;
* UROP&lt;br /&gt;
* Sep 2010 - Jan 2012&lt;br /&gt;
 |width=25|&lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Pic.jpg|right|100px]] &lt;br /&gt;
[[User:Eris Bame|&#039;&#039;&#039;Eris Bame&#039;&#039;&#039;]]&lt;br /&gt;
* Technical Co-op Assistant &lt;br /&gt;
* July-Dec 2010&lt;br /&gt;
 |width=25|&lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:ksulli.jpg|right|80px]]&lt;br /&gt;
[[User:Katherine Sullivan|&#039;&#039;&#039;Katie Sullivan&#039;&#039;&#039;]] &lt;br /&gt;
* Technical Co-op Assistant &lt;br /&gt;
* Jan-June 2011&lt;br /&gt;
 |- style=&amp;quot;height:25px;&amp;quot;&lt;br /&gt;
 | colspan=&amp;quot;5&amp;quot; |&lt;br /&gt;
 |- style=&amp;quot;height:150xp;&amp;quot; &lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:elliott.j.jpg|right|80px]]&lt;br /&gt;
[[User:Justin Elliott|&#039;&#039;&#039;Justin Elliott&#039;&#039;&#039;]]&lt;br /&gt;
* Technical Co-op Assistant &lt;br /&gt;
* Jan-June 2011&lt;br /&gt;
 |width=25|&lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:270627 10150316369264740 594529739 9434885 4028882 n.jpg|right|100px]]&lt;br /&gt;
[[User:Kaitlyn N. Sanders|&#039;&#039;&#039;Kaitlyn Sanders&#039;&#039;&#039;]] &lt;br /&gt;
* Technical Co-op Assistant &lt;br /&gt;
* July-Dec 2011&lt;br /&gt;
 |width=25|&lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Lebowitz.j.jpg|right|100px]]&lt;br /&gt;
[[User:Jessica L. Lebowitz|&#039;&#039;&#039;Jessica Lebowitz&#039;&#039;&#039;]] &lt;br /&gt;
* Technical Co-op Assistant&lt;br /&gt;
* July-Dec 2011&lt;br /&gt;
 |- style=&amp;quot;height:25px;&amp;quot;&lt;br /&gt;
 | colspan=&amp;quot;5&amp;quot; |&lt;br /&gt;
 |- style=&amp;quot;height:150xp;&amp;quot; &lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Ln123.png|right|80px]]&lt;br /&gt;
[[User:Linda Nguyen|&#039;&#039;&#039;Linda Nguyen&#039;&#039;&#039;]] &lt;br /&gt;
* Technical Co-op Assistant&lt;br /&gt;
* Jan-June 2012&lt;br /&gt;
 |width=25|&lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Pictureforwebsite.JPG|right|80px]]&lt;br /&gt;
[[User:Margaret Minnig|&#039;&#039;&#039;Margaret Minnig&#039;&#039;&#039;]] &lt;br /&gt;
* Technical Co-op Assistant&lt;br /&gt;
* July-Dec 2012&lt;br /&gt;
 |width=25|&lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Photo_for_website.JPG‎|right|80px]]&lt;br /&gt;
[[User:Kate Tracka|&#039;&#039;&#039;Kate Tracka&#039;&#039;&#039;]] &lt;br /&gt;
* Technical Co-op Assistant&lt;br /&gt;
* July-Dec 2012&lt;br /&gt;
 |- style=&amp;quot;height:25px;&amp;quot;&lt;br /&gt;
 | colspan=&amp;quot;5&amp;quot; |&lt;br /&gt;
 |- style=&amp;quot;height:150xp;&amp;quot; &lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:PDP2.jpg|right|80px]]&lt;br /&gt;
[[User:Paraj Patel|&#039;&#039;&#039;Paraj Patel&#039;&#039;&#039;]]&lt;br /&gt;
* Technical Co-op Assistant&lt;br /&gt;
* Jan-Jun 2013&lt;br /&gt;
 |width=25|&lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:millet.jpg|right|80px]]&lt;br /&gt;
[[User:Pierrick Millet|&#039;&#039;&#039;Pierrick Millet&#039;&#039;&#039;]]&lt;br /&gt;
* Technical Co-op Assistant&lt;br /&gt;
* Jan-Jun 2013&lt;br /&gt;
 |width=25|&lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:BMC_stockScientist.jpg|right|100px]]&lt;br /&gt;
[[User:Zach Banks|&#039;&#039;&#039;Zach Banks&#039;&#039;&#039;]] &lt;br /&gt;
* UROP&lt;br /&gt;
* &lt;br /&gt;
 |- style=&amp;quot;height:25px;&amp;quot;&lt;br /&gt;
 | colspan=&amp;quot;5&amp;quot; |&lt;br /&gt;
 |- style=&amp;quot;height:150xp;&amp;quot; &lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Machado.jpg|right|100px]]&lt;br /&gt;
[[User:ENTER HERE|&#039;&#039;&#039;Ashley Machado&#039;&#039;&#039;]]&lt;br /&gt;
* Technical Co-op&lt;br /&gt;
* July-Dec 2013&lt;br /&gt;
Sample Prep&lt;br /&gt;
 |width=25|&lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Soltoff.jpg|right|100px]]&lt;br /&gt;
[[User:Alexander E. Soltoff|&#039;&#039;&#039;Alexander Soltoff&#039;&#039;&#039;]]&lt;br /&gt;
* Technical Co-op&lt;br /&gt;
* July-Dec 2013&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
|width=25|&lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Ani_Webb.jpg|right|100px]]&lt;br /&gt;
[[User:Ani S. Webb|&#039;&#039;&#039;Ani Webb&#039;&#039;&#039;]]&lt;br /&gt;
* Technical Co-op&lt;br /&gt;
* Jan-June 2014&lt;br /&gt;
 |- style=&amp;quot;height:25px;&amp;quot;&lt;br /&gt;
 | colspan=&amp;quot;5&amp;quot; |&lt;br /&gt;
 |- style=&amp;quot;height:150xp;&amp;quot; &lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:OWWEmblem.png|right|100px]]&lt;br /&gt;
[[User:Samuel Kaplan|&#039;&#039;&#039;Samuel Kaplan&#039;&#039;&#039;]]&lt;br /&gt;
* Co-op&lt;br /&gt;
* Technical Co-op&lt;br /&gt;
* Jan-June 2014&lt;br /&gt;
  |width=25|&lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:AdamP.jpg|right|100px]]&lt;br /&gt;
[[User:Adam Perez|&#039;&#039;&#039;Adam Perez&#039;&#039;&#039;]]&lt;br /&gt;
* Co-op&lt;br /&gt;
* Phone: x54533&lt;br /&gt;
* Email: perezad@mit.edu&lt;br /&gt;
Quality Control&lt;br /&gt;
|width=25|&lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:JonathanPicture.JPG|right|100px]]&lt;br /&gt;
[[User:Jonathan Olson|&#039;&#039;&#039;Jonathan&#039;&#039;&#039;]]&lt;br /&gt;
*Co-op&lt;br /&gt;
* Phone: x54533&lt;br /&gt;
* Email: olsonj@mit.edu&lt;br /&gt;
Quality Control&lt;br /&gt;
|width=25|&lt;br /&gt;
|- style=&amp;quot;height:25px;&amp;quot;&lt;br /&gt;
 | colspan=&amp;quot;5&amp;quot; |&lt;br /&gt;
 |- style=&amp;quot;height:150xp;&amp;quot; &lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Rachel_Stoddard.jpg|right|100px]]&lt;br /&gt;
[[User:Rachel Stoddard|&#039;&#039;&#039;Rachel Stoddard&#039;&#039;&#039;]]&lt;br /&gt;
* Co-op&lt;br /&gt;
*Quality Control&lt;br /&gt;
*June 2015-December 2015&lt;br /&gt;
 |width=25|&lt;br /&gt;
 &lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Rob_Freeman.png|right|100px]]&lt;br /&gt;
[[User:Robert H. Freeman|&#039;&#039;&#039;Robert Freeman&#039;&#039;&#039;]]&lt;br /&gt;
* Co -op&lt;br /&gt;
*Sample Prep&lt;br /&gt;
*July 2015-January 2016&lt;br /&gt;
 |width=25|&lt;br /&gt;
 &lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Image1-2.jpg|right|100px]]&lt;br /&gt;
[[User:Roberto Mennuti|&#039;&#039;&#039;Roberto Mennuti&#039;&#039;&#039;]]&lt;br /&gt;
* Co-op&lt;br /&gt;
*Quality Control&lt;br /&gt;
*January-July 2016&lt;br /&gt;
 |width=25|&lt;br /&gt;
&lt;br /&gt;
|- style=&amp;quot;height:25px;&amp;quot;&lt;br /&gt;
 | colspan=&amp;quot;5&amp;quot; |&lt;br /&gt;
 |- style=&amp;quot;height:150xp;&amp;quot; &lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:20160126_102243.JPG|right|100px]]&lt;br /&gt;
[[User:Kathryn Larkin|&#039;&#039;&#039;Kathryn Larkin&#039;&#039;&#039;]] &lt;br /&gt;
* Co-op&lt;br /&gt;
*Quality Control&lt;br /&gt;
*January-July 2016&lt;br /&gt;
 |width=25|&lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:20160126_102242-1.jpg|right|100px]]&lt;br /&gt;
[[User:Asa M. Budnick|&#039;&#039;&#039;Asa Budnick&#039;&#039;&#039;]] &lt;br /&gt;
* Co-op&lt;br /&gt;
* Library Preparation&lt;br /&gt;
* January-July 2016&lt;br /&gt;
  |width=25|&lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Kevin_Lema.jpg|right|100px]]&lt;br /&gt;
[[User:Kevin Lema|&#039;&#039;&#039;Kevin Lema&#039;&#039;&#039;]]&lt;br /&gt;
* Co-op&lt;br /&gt;
*Quality Control&lt;br /&gt;
*July-December 2016&lt;br /&gt;
 |width=25|&lt;br /&gt;
&lt;br /&gt;
|- style=&amp;quot;height:25px;&amp;quot;&lt;br /&gt;
 | colspan=&amp;quot;5&amp;quot; |&lt;br /&gt;
 |- style=&amp;quot;height:150xp;&amp;quot; &lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:alexis_hester.JPG|right|100px]]&lt;br /&gt;
[[User:Alexis Hester|&#039;&#039;&#039;Alexis Hester&#039;&#039;&#039;]]&lt;br /&gt;
* Co-op&lt;br /&gt;
*Quality Control&lt;br /&gt;
*July-December 2016&lt;br /&gt;
 |width=25|&lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Coriposner.jpg|right|100px]]&lt;br /&gt;
[[User:Cori Posner|&#039;&#039;&#039;Cori Posner&#039;&#039;&#039;]]&lt;br /&gt;
* Co-op&lt;br /&gt;
*Quality Control&lt;br /&gt;
*July-December 2016&lt;br /&gt;
 |width=25|&lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:CharlieMintz.jpg|right|100px]]&lt;br /&gt;
[[User:Charlie Mintz|&#039;&#039;&#039;Charlie Mintz&#039;&#039;&#039;]]&lt;br /&gt;
* Co-op&lt;br /&gt;
*Quality Control&lt;br /&gt;
*January-July 2017&lt;br /&gt;
 |width=25|&lt;br /&gt;
&amp;lt;!--beginning of new line--&amp;gt;&lt;br /&gt;
 |- style=&amp;quot;height:25px;&amp;quot;&lt;br /&gt;
 | colspan=&amp;quot;5&amp;quot; |&lt;br /&gt;
 |- style=&amp;quot;height:150xp;&amp;quot; &lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Meghna.jpeg|right|100px]]&lt;br /&gt;
[[User:Meghna Kuppuraju|&#039;&#039;&#039;Meghna Kuppuraju&#039;&#039;&#039;]]&lt;br /&gt;
* Co-op&lt;br /&gt;
*Quality Control&lt;br /&gt;
*January-July 2017&lt;br /&gt;
 |width=25|&lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Viren.png|right|100px]]&lt;br /&gt;
[[User:Viren Kumar Dayal |&#039;&#039;&#039;Viren Kumar Dayal&#039;&#039;&#039;]]&lt;br /&gt;
* Co-op&lt;br /&gt;
*Quality Control&lt;br /&gt;
*January-July 2017&lt;br /&gt;
&lt;br /&gt;
 |width=25|&lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Duncan.jpg|right|100px]]&lt;br /&gt;
[[User:Duncan Andrew Locke|&#039;&#039;&#039;Duncan Locke&#039;&#039;&#039;]] - COOP&lt;br /&gt;
* Phone: x54533&lt;br /&gt;
* Email: lockedu@mit.edu&lt;br /&gt;
Specialty: Quality control&lt;br /&gt;
Fall 2017&lt;br /&gt;
&lt;br /&gt;
|- style=&amp;quot;height:25px;&amp;quot;&lt;br /&gt;
 | colspan=&amp;quot;5&amp;quot; |&lt;br /&gt;
 |- style=&amp;quot;height:150xp;&amp;quot; &lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
&lt;br /&gt;
[[User: Abigail Blake|&#039;&#039;&#039;Abigail Blake&#039;&#039;&#039;]] - COOP&lt;br /&gt;
* Phone: x54533&lt;br /&gt;
* Email: ablake@mit.edu&lt;br /&gt;
Specialty: Quality Control&lt;br /&gt;
Fall 2017&lt;br /&gt;
&lt;br /&gt;
 |width=25|&lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
&lt;br /&gt;
[[Image:A.jpg|right|100px]]&lt;br /&gt;
[[User:Meghan Masotti|&#039;&#039;&#039;Meghan Masotti&#039;&#039;&#039;]] - COOP&lt;br /&gt;
* Phone: x54533&lt;br /&gt;
* Email: mmasotti@mit.edu&lt;br /&gt;
Specialty: Quality Control &amp;amp;Sample Prep.&lt;br /&gt;
Spring 2018&lt;br /&gt;
&lt;br /&gt;
 |width=25|&lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
&lt;br /&gt;
[[Image:EMILY.jpg|right|100px]]&lt;br /&gt;
[[User:Emily Evangelista|&#039;&#039;&#039;Emily Evangelista&#039;&#039;&#039;]] - COOP&lt;br /&gt;
* Co-op&lt;br /&gt;
*Quality Control&lt;br /&gt;
*January-July 2018&lt;br /&gt;
|- style=&amp;quot;height:25px;&amp;quot;&lt;br /&gt;
 | colspan=&amp;quot;5&amp;quot; |&lt;br /&gt;
 |- style=&amp;quot;height:150xp;&amp;quot; &lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
&lt;br /&gt;
[[Image:INSERT|right|100px]]&lt;br /&gt;
[[User:Melissa Franco|&#039;&#039;&#039;Melissa Franco&#039;&#039;&#039;]] - COOP&lt;br /&gt;
* Co-op&lt;br /&gt;
*Quality Control&lt;br /&gt;
*January-July 2018&lt;br /&gt;
&lt;br /&gt;
 |width=25|&lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
&lt;br /&gt;
[[Image:Kr124325.jpg |right|100px]]&lt;br /&gt;
[[User:Orourke.k|&#039;&#039;&#039;Kelly O&#039;Rourke&#039;&#039;&#039;]] - COOP&lt;br /&gt;
* Co-op&lt;br /&gt;
*Sample Prep&lt;br /&gt;
*January-July 2018&lt;br /&gt;
&lt;br /&gt;
 |width=25|&lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
&lt;br /&gt;
[[Image:JULIA.jpg|right|100px]]&lt;br /&gt;
[[User:Julia Breed|&#039;&#039;&#039;Julia Breed&#039;&#039;&#039;]] - COOP &lt;br /&gt;
* Co-op&lt;br /&gt;
*Quality control&lt;br /&gt;
*July-December 2018&lt;br /&gt;
&lt;br /&gt;
|- style=&amp;quot;height:25px;&amp;quot;&lt;br /&gt;
 | colspan=&amp;quot;5&amp;quot; |&lt;br /&gt;
 |- style=&amp;quot;height:150xp;&amp;quot; &lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
&lt;br /&gt;
[[Image:WillPhoto.jpeg|right|100px]]&lt;br /&gt;
[[User:William Bonaventura|&#039;&#039;&#039;William Bonaventura&#039;&#039;&#039;]] - COOP &lt;br /&gt;
* Co-op&lt;br /&gt;
*Quality Control&lt;br /&gt;
*July-December 2018&lt;br /&gt;
&lt;br /&gt;
 |width=25|&lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
&lt;br /&gt;
[[Image:JOSEPH.jpg |right|100px]]&lt;br /&gt;
[[User:Joekatz|&#039;&#039;&#039;Joseph Katz&#039;&#039;&#039;]] - COOP&lt;br /&gt;
* Co-op&lt;br /&gt;
*Sample Prep&lt;br /&gt;
*July-December 2018&lt;br /&gt;
&lt;br /&gt;
|width=25|&lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
&lt;br /&gt;
[[Image:Dang.png|right|100px]]&lt;br /&gt;
[[User:Dang-Huy Nguyen|&#039;&#039;&#039;Dang-Huy Nguyen&#039;&#039;&#039;]] - COOP Spring 2019&lt;br /&gt;
* Phone: x54533&lt;br /&gt;
* Email: dhnguyen@mit.edu&lt;br /&gt;
Specialty: Quality control&lt;br /&gt;
Spring 2019&lt;br /&gt;
|- style=&amp;quot;height:25px;&amp;quot;&lt;br /&gt;
 | colspan=&amp;quot;5&amp;quot; |&lt;br /&gt;
 |- style=&amp;quot;height:150xp;&amp;quot; &lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
&lt;br /&gt;
[[Image:VindyaPhoto.jpeg|right|100px]]&lt;br /&gt;
[[User:Vindya Tumati|&#039;&#039;&#039;Vindya Tumati&#039;&#039;&#039;]] - COOP Spring 2019&lt;br /&gt;
* Phone: x54533&lt;br /&gt;
* Email: vtumati@mit.edu&lt;br /&gt;
Specialty: Quality Control&lt;br /&gt;
Spring 2019&lt;br /&gt;
|width=25|&lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:taeykim.jpg|right|100px]]&lt;br /&gt;
[[User:Taeyun Kim|&#039;&#039;&#039;Taeyun Kim&#039;&#039;&#039;]] - COOP Fall 2019&lt;br /&gt;
* Phone: x54533&lt;br /&gt;
* Email: taeykim@mit.edu&lt;br /&gt;
Specialty: Quality control&lt;br /&gt;
Fall 2019&lt;br /&gt;
|width=25|&lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Simrah.jpg|right|100px]]&lt;br /&gt;
[[User:Simrah Ahmed|&#039;&#039;&#039;Simrah Ahmed&#039;&#039;&#039;]] - COOP Fall 2019&lt;br /&gt;
* Phone: x54533&lt;br /&gt;
* Email: ahmedsim@mit.edu&lt;br /&gt;
Specialty: Quality control&lt;br /&gt;
Fall 2019&lt;br /&gt;
 |- style=&amp;quot;height:25px;&amp;quot;&lt;br /&gt;
 | colspan=&amp;quot;5&amp;quot; |&lt;br /&gt;
 |- style=&amp;quot;height:150xp;&amp;quot; &lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Thy.png|right|100px]]&lt;br /&gt;
* Janssy Garcia - Co-op Spring 2020&lt;br /&gt;
* Thy Nguyen (pictured) - Co-op Spring 2020&lt;br /&gt;
|width=25|&lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Merline.jpg|right|100px]]&lt;br /&gt;
[[User:Challee|&#039;&#039;&#039;Merline Senat&#039;&#039;&#039;]] - Co-op Fall 2020&lt;br /&gt;
* Phone: x54533&lt;br /&gt;
* Email: merline@mit.edu&lt;br /&gt;
|width=25|&lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Devin.jpg|right|100px]]&lt;br /&gt;
[[User:Devin|&#039;&#039;&#039;Devin Chatterjee&#039;&#039;&#039;]] - Co-op Fall 2020&lt;br /&gt;
* Phone: x54533&lt;br /&gt;
* Email: dchatt@mit.edu&lt;br /&gt;
|- style=&amp;quot;height:25px;&amp;quot;&lt;br /&gt;
| colspan=&amp;quot;5&amp;quot; |&lt;br /&gt;
|- style=&amp;quot;height:150xp;&amp;quot; &lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Mali.jpg|right|100px]]&lt;br /&gt;
[[User:Mali|&#039;&#039;&#039;Mali DiMeo&#039;&#039;&#039;]] - Co-op Spring 2021&lt;br /&gt;
* Phone: x54533&lt;br /&gt;
* Email: mdimeo@mit.edu&lt;br /&gt;
|width=25|&lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Tram.jpg|right|100px]]&lt;br /&gt;
[[User:Tram|&#039;&#039;&#039;Tram Huynh&#039;&#039;&#039;]] - Co-op Spring 2021&lt;br /&gt;
* Phone: x54533&lt;br /&gt;
* Email: thuynh@mit.edu&lt;br /&gt;
|width=25|&lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Owen.jpg|right|100px]]&lt;br /&gt;
[[User:Owen|&#039;&#039;&#039;Owen Spencer&#039;&#039;&#039;]] - Co-op Fall 2021&lt;br /&gt;
* Phone: x54533&lt;br /&gt;
* Email: ospencer@mit.edu&lt;br /&gt;
 |width=25|&lt;br /&gt;
 |- style=&amp;quot;height:25px;&amp;quot;&lt;br /&gt;
 | colspan=&amp;quot;5&amp;quot; |&lt;br /&gt;
 |- style=&amp;quot;height:150xp;&amp;quot;&lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Victoria.jpg|right|100px]]&lt;br /&gt;
[[User:Victoria|&#039;&#039;&#039;Victoria Russo&#039;&#039;&#039;]] - Co-op Fall 2021&lt;br /&gt;
* Phone: x54533&lt;br /&gt;
* Email: russov@mit.edu &lt;br /&gt;
|width=25|&lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:baifan-1.jpg|right|100px]]&lt;br /&gt;
[[User:Baifan|&#039;&#039;&#039;Baifan Zhou&#039;&#039;&#039;]] - Co-op Fall 2022&lt;br /&gt;
* Phone: x54533&lt;br /&gt;
* Email: baifan@mit.edu&lt;br /&gt;
|width=25|&lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:egihasani.jpg|right|100px]]&lt;br /&gt;
[[User:Egi|&#039;&#039;&#039;Egi Hasani&#039;&#039;&#039;]] - Co-op Fall 2022&lt;br /&gt;
* Phone: x54533&lt;br /&gt;
* Email: ehasani@mit.edu&lt;br /&gt;
|width=25|&lt;br /&gt;
 |width=25|&lt;br /&gt;
 |- style=&amp;quot;height:25px;&amp;quot;&lt;br /&gt;
 | colspan=&amp;quot;5&amp;quot; |&lt;br /&gt;
 |- style=&amp;quot;height:150xp;&amp;quot;&lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:JakeS.jpg|right|100px]]&lt;br /&gt;
[[User:Jake3784|&#039;&#039;&#039;Jake Schissel&#039;&#039;&#039;]] - FAIRdata Co-op Fall 2022&lt;br /&gt;
* Email: jschisse@mit.edu&lt;br /&gt;
 |width=25|&lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:SalmaAlawi.png|right|100px]]&lt;br /&gt;
[[User:Salma|&#039;&#039;&#039;Salma Alawi&#039;&#039;&#039;]] - Data Co-op Spring 2023&lt;br /&gt;
* Email: salawi@mit.edu&lt;br /&gt;
 |width=25|&lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Liammccoart.jpg|right|100px]]&lt;br /&gt;
[[User:Liam|&#039;&#039;&#039;Liam McCoart&#039;&#039;&#039;]] - Co-op Spring 2023&lt;br /&gt;
* Email: mccoart@mit.edu&lt;br /&gt;
|width=25|&lt;br /&gt;
 |width=25|&lt;br /&gt;
 |- style=&amp;quot;height:25px;&amp;quot;&lt;br /&gt;
 | colspan=&amp;quot;5&amp;quot; |&lt;br /&gt;
 |- style=&amp;quot;height:150xp;&amp;quot;&lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Snigdha.jpg|right|100px]]&lt;br /&gt;
[[User:Snigdha|&#039;&#039;&#039;Snigdha Patra&#039;&#039;&#039;]] - Co-op Spring 2023&lt;br /&gt;
* Email: patras@mit.edu&lt;br /&gt;
|width=25|&lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Simrensamba.jpg|right|100px]]&lt;br /&gt;
[[User:Simren|&#039;&#039;&#039;Simren Samba&#039;&#039;&#039;]] - Co-op Fall 2023&lt;br /&gt;
* Phone: x54533&lt;br /&gt;
* Email: ssamba@mit.edu&lt;br /&gt;
|width=25|&lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:ENiemiec.jpeg|right|100px]]&lt;br /&gt;
[[User:Elizabeth|&#039;&#039;&#039;Elizabeth Niemiec&#039;&#039;&#039;]] - Co-op Fall 2023&lt;br /&gt;
* Phone: x54533&lt;br /&gt;
* Email: eniemiec@mit.edu&lt;br /&gt;
&lt;br /&gt;
 |- style=&amp;quot;height:25px;&amp;quot;&lt;br /&gt;
 | colspan=&amp;quot;5&amp;quot; |&lt;br /&gt;
 |- style=&amp;quot;height:150xp;&amp;quot; &lt;br /&gt;
 |width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
&lt;br /&gt;
[[Image:EuniceK.jpg|right|100px]]&lt;br /&gt;
[[User:kooe|&#039;&#039;&#039;Eunice Koo&#039;&#039;&#039;]]&lt;br /&gt;
* Co-op- Data - Fall 2023&lt;br /&gt;
* Email: kooe@mit.edu&lt;br /&gt;
&lt;br /&gt;
|width=25|&lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
&lt;br /&gt;
[[Image:BMClindatran.jpeg|right|100px]]&lt;br /&gt;
[[User:lttran|&#039;&#039;&#039;Linda Tran&#039;&#039;&#039;]] - Co-op Spring 2024&lt;br /&gt;
* Phone x54533&lt;br /&gt;
* Email: lttran@mit.edu&lt;br /&gt;
&lt;br /&gt;
|width=25|&lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Gideon_mitcoop.jpeg|right|100px]]&lt;br /&gt;
[[User:Gideon|&#039;&#039;&#039;Gideon Donahue&#039;&#039;&#039;]] - Co-op Spring 2024&lt;br /&gt;
* Phone: x54533&lt;br /&gt;
* Email: gideon03@mit.edu&lt;br /&gt;
&lt;br /&gt;
|- style=&amp;quot;height:25px;&amp;quot;&lt;br /&gt;
 | colspan=&amp;quot;5&amp;quot; |&lt;br /&gt;
 |- style=&amp;quot;height:150xp;&amp;quot; &lt;br /&gt;
&amp;lt;!-- This gives us a &amp;quot;linebreak&amp;quot; of sorts, the colspan indicated is 5 which means there should only be five cells in a row (three bios and two |width=25| cells), anything else will mess up this table formatting, but that can be fixed by resetting the colspan for all &amp;quot;linebreaks&amp;quot; (rowspan can be used similarly) --&amp;gt;&lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Neil_.jpeg|right|100px]]&lt;br /&gt;
[[User:Neil|&#039;&#039;&#039;Neil Sheth&#039;&#039;&#039;]] - Co-op Single Cell Fall 2023-Fall 2024&lt;br /&gt;
* Phone: x54533&lt;br /&gt;
* Email: neil98@mit.edu&lt;br /&gt;
&lt;br /&gt;
|width=25|&lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
&lt;br /&gt;
[[Image:jalyon.jpeg|right|100px]]&lt;br /&gt;
[[User:Jalyon|&#039;&#039;&#039;Jared Lyon&#039;&#039;&#039;]] - Drylab Co-op Spring 2024&lt;br /&gt;
* Email: jalyon@mit.edu&lt;br /&gt;
&lt;br /&gt;
|width=25|&lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
&lt;br /&gt;
[[Image:sara1463.jpeg|right|100px]]&lt;br /&gt;
[[User:Gideon|&#039;&#039;&#039;Sara Farinha&#039;&#039;&#039;]] - Wetlab Co-op Fall 2024&lt;br /&gt;
* Phone: x54533&lt;br /&gt;
* Email: sara1463@mit.edu&lt;br /&gt;
&lt;br /&gt;
|- style=&amp;quot;height:25px;&amp;quot;&lt;br /&gt;
 | colspan=&amp;quot;5&amp;quot; |&lt;br /&gt;
 |- style=&amp;quot;height:150xp;&amp;quot; &lt;br /&gt;
&amp;lt;!-- This gives us a &amp;quot;linebreak&amp;quot; of sorts, the colspan indicated is 5 which means there should only be five cells in a row (three bios and two |width=25| cells), anything else will mess up this table formatting, but that can be fixed by resetting the colspan for all &amp;quot;linebreaks&amp;quot; (rowspan can be used similarly) --&amp;gt;&lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
&lt;br /&gt;
[[Image:bolducje.jpeg|right|100px]]&lt;br /&gt;
[[User:bolducnu.jpg|&#039;&#039;&#039;Jessica Bolduc&#039;&#039;&#039;]] - Wetlab Co-op Fall 2024&lt;br /&gt;
* Phone x54533&lt;br /&gt;
* Email: bolducje@mit.edu&lt;br /&gt;
&lt;br /&gt;
|width=25|&lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:frankie.jpeg|right|100px]]&lt;br /&gt;
[[User:Jalyon|&#039;&#039;&#039;Francesca Licciardello&#039;&#039;&#039;]]&lt;br /&gt;
* Drylab Co-op Fall 2024&lt;br /&gt;
* Email: fl13@mit.edu&lt;br /&gt;
&lt;br /&gt;
|width=25|&lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Hannah.jpg|right|100px]]&lt;br /&gt;
[[User:hannahj4|&#039;&#039;&#039;Hannah Jackson&#039;&#039;&#039;]] - Wetlab Co-op Spring 2025&lt;br /&gt;
* Phone: x54533&lt;br /&gt;
* Email: hannahj4@mit.edu&lt;br /&gt;
&lt;br /&gt;
|- style=&amp;quot;height:25px;&amp;quot;&lt;br /&gt;
 | colspan=&amp;quot;5&amp;quot; |&lt;br /&gt;
 |- style=&amp;quot;height:150xp;&amp;quot; &lt;br /&gt;
&amp;lt;!-- This gives us a &amp;quot;linebreak&amp;quot; of sorts, the colspan indicated is 5 which means there should only be five cells in a row (three bios and two |width=25| cells), anything else will mess up this table formatting, but that can be fixed by resetting the colspan for all &amp;quot;linebreaks&amp;quot; (rowspan can be used similarly) --&amp;gt;&lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:maddie.jpeg|right|100px]]&lt;br /&gt;
[[User:madelin9|&#039;&#039;&#039;Madeline Fiedler&#039;&#039;&#039;]] - Wetlab Co-op Spring 2025&lt;br /&gt;
* Phone x54533&lt;br /&gt;
* Email: madelin9@mit.edu&lt;br /&gt;
|width=25|&lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:Marley.jpg|right|100px]]&lt;br /&gt;
[[User:marleyoc|&#039;&#039;&#039;Marley O&#039;Connor&#039;&#039;&#039;]] - Co-op&lt;br /&gt;
* Phone: x54533&lt;br /&gt;
* Email: marleyoc@mit.edu&lt;br /&gt;
|width=25|&lt;br /&gt;
|width=350 style=&amp;quot;border: 1px solid purple;&amp;quot;|&lt;br /&gt;
[[Image:jackie.jpg|right|100px]]&lt;br /&gt;
[[User:pottlej|&#039;&#039;&#039;Jacqueline Pottle&#039;&#039;&#039;]] - Co-op&lt;br /&gt;
* Phone x54533&lt;br /&gt;
* Email: pottlej@mit.edu&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
|}&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=File:Uday.jpeg&amp;diff=131441</id>
		<title>File:Uday.jpeg</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=File:Uday.jpeg&amp;diff=131441"/>
		<updated>2026-05-07T19:06:38Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:PacBio_Library_Preparation&amp;diff=131393</id>
		<title>BioMicroCenter:PacBio Library Preparation</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:PacBio_Library_Preparation&amp;diff=131393"/>
		<updated>2026-04-21T01:49:36Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: Redirect to consolidated BioMicroCenter:LongRead page&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;#REDIRECT [[BioMicroCenter:LongRead]]&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:PacBio&amp;diff=131392</id>
		<title>BioMicroCenter:PacBio</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:PacBio&amp;diff=131392"/>
		<updated>2026-04-21T01:49:35Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: Redirect to consolidated BioMicroCenter:LongRead page&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;#REDIRECT [[BioMicroCenter:LongRead]]&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:NanoPore_Library_Prep&amp;diff=131391</id>
		<title>BioMicroCenter:NanoPore Library Prep</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:NanoPore_Library_Prep&amp;diff=131391"/>
		<updated>2026-04-21T01:49:35Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: Redirect to consolidated BioMicroCenter:LongRead page&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;#REDIRECT [[BioMicroCenter:LongRead]]&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:Oxford_Nanopore_Technologies&amp;diff=131390</id>
		<title>BioMicroCenter:Oxford Nanopore Technologies</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:Oxford_Nanopore_Technologies&amp;diff=131390"/>
		<updated>2026-04-21T01:49:34Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: Redirect to consolidated BioMicroCenter:LongRead page&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;#REDIRECT [[BioMicroCenter:LongRead]]&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:Oxford_Nanopore&amp;diff=131389</id>
		<title>BioMicroCenter:Oxford Nanopore</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:Oxford_Nanopore&amp;diff=131389"/>
		<updated>2026-04-21T01:49:33Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: Redirect to consolidated BioMicroCenter:LongRead page&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;#REDIRECT [[BioMicroCenter:LongRead]]&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:LongRead&amp;diff=131388</id>
		<title>BioMicroCenter:LongRead</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:LongRead&amp;diff=131388"/>
		<updated>2026-04-21T01:49:33Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: Create consolidated long read sequencing page (ONT PromethION, PacBio Revio)&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;{{BioMicroCenter}}&lt;br /&gt;
&lt;br /&gt;
The MIT BioMicro Center offers long-read sequencing on two platforms: Oxford Nanopore Technologies PromethION P2 Solo and PacBio Revio. Both platforms excel at applications where read length matters — de novo assembly, structural variant detection, full-length isoform sequencing, and epigenetic base modification — but differ significantly in chemistry, accuracy profile, throughput, and library requirements. &lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;background:#f8f9fa; border:1px solid #ccc; padding:12px 16px; margin:12px 0;&amp;quot;&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;Choosing a platform?&#039;&#039;&#039; Jump to [[#PLATFORM_COMPARISON|platform comparison]] below, or contact [mailto:biomicro@mit.edu biomicro@mit.edu].&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== OVERVIEW ==&lt;br /&gt;
&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; style=&amp;quot;width:100%; text-align:center;&amp;quot;&lt;br /&gt;
|-&lt;br /&gt;
! style=&amp;quot;width:18%; background:#dce8f5;&amp;quot; | PLATFORM&lt;br /&gt;
! style=&amp;quot;width:22%; background:#dce8f5;&amp;quot; | OUTPUT / FLOWCELL&lt;br /&gt;
! style=&amp;quot;width:20%; background:#dce8f5;&amp;quot; | TYPICAL READ LENGTH&lt;br /&gt;
! style=&amp;quot;width:20%; background:#dce8f5;&amp;quot; | AVAILABILITY&lt;br /&gt;
|-&lt;br /&gt;
| [[Image:PromethION.jpg|center|120px]] &amp;lt;br&amp;gt; &#039;&#039;&#039;PromethION P2 Solo&#039;&#039;&#039;&lt;br /&gt;
| ~50–150 Gb &amp;lt;br&amp;gt; (up to 290 Gb theoretical max)&lt;br /&gt;
| Variable; N50 typically 10–50 kb &amp;lt;br&amp;gt; Ultra-long reads &amp;gt;100 kb possible&lt;br /&gt;
| In-house; MIT and external users&lt;br /&gt;
|-&lt;br /&gt;
| [[Image:Sequel.jpeg|center|120px]] &amp;lt;br&amp;gt; &#039;&#039;&#039;PacBio Revio&#039;&#039;&#039;&lt;br /&gt;
| ~80 Gb HiFi &amp;lt;br&amp;gt; per SMRTcell&lt;br /&gt;
| HiFi: typically 8-16kb kb &amp;lt;br&amp;gt;&lt;br /&gt;
| style=&amp;quot;font-size:90%; color:#555;&amp;quot; | Via collaboration with nearby academic cores.&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
== SEQUENCING PLATFORMS ==&lt;br /&gt;
&lt;br /&gt;
&amp;lt;!-- ═══════════════════════════ OXFORD NANOPORE TECHNOLOGIES ════════════════ --&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Oxford Nanopore Technologies ==&lt;br /&gt;
&amp;lt;span id=&amp;quot;ONT&amp;quot;&amp;gt;&amp;lt;/span&amp;gt;&lt;br /&gt;
&lt;br /&gt;
The BioMicro Center operates two [https://nanoporetech.com/products/sequence/promethion-2-solo PromethION P2 Solo] instruments from [https://nanoporetech.com Oxford Nanopore Technologies], each capable of running two flowcells concurrently. Nanopore sequencing passes DNA or RNA through a protein pore under voltage; current fluctuations as bases traverse the pore are decoded in real time into sequence and base modification calls. Unlike optical platforms, there is no theoretical read-length limit — the BMC has seen reads exceeding 300 kbp. The platform natively detects base modifications (methylation, hydroxymethylation, and others) without bisulfite conversion or additional chemistry. BMC runs each flowcell is run to completion and not reused.&lt;br /&gt;
&lt;br /&gt;
=== PromethION P2 Solo — Sequencing ===&lt;br /&gt;
&lt;br /&gt;
{|&lt;br /&gt;
 |- style=&amp;quot;vertical-align:top;&amp;quot;&lt;br /&gt;
 | style=&amp;quot;width:370px;&amp;quot; |&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
 ! Service&lt;br /&gt;
 ! Nanopore Sequencing&lt;br /&gt;
 |-&lt;br /&gt;
 | INPUT || Completed Nanopore libraries&lt;br /&gt;
 |-&lt;br /&gt;
 | MIN CONCENTRATION || Library-type dependent; read count not guaranteed&lt;br /&gt;
 |-&lt;br /&gt;
 | INCLUDED SERVICES&lt;br /&gt;
 |&lt;br /&gt;
* Quality Control: UV-vis measurement&lt;br /&gt;
* Nanopore Sequencing&lt;br /&gt;
* Demultiplexing&lt;br /&gt;
* Modified basecalling available on request&lt;br /&gt;
* Adaptive sampling (FASTA + optional BED file required)&lt;br /&gt;
 |-&lt;br /&gt;
 | ADDITIONAL SERVICES ||&lt;br /&gt;
* [[BioMicroCenter:NanoPore_Library_Prep|Nanopore library preparation]]&lt;br /&gt;
 |-&lt;br /&gt;
 | DATA FORMATS&lt;br /&gt;
 |&lt;br /&gt;
* FASTQ (stored 90 days, then archived)&lt;br /&gt;
* FAST5 / POD5 (stored 30 days, then deleted)&lt;br /&gt;
* Assorted Nanopore QC&lt;br /&gt;
 |-&lt;br /&gt;
 | PRICING || [[MIT:Pricing]] · [[BioMicroCenter:Pricing]]&lt;br /&gt;
 |-&lt;br /&gt;
 | SUBMISSION&lt;br /&gt;
 |&lt;br /&gt;
* MIT – [https://mit.ilabsolutions.com/service_item/new/3381?spt_id=3863 iLabs]&lt;br /&gt;
* External – [[BioMicroCenter:Forms|form]]&lt;br /&gt;
 |-&lt;br /&gt;
|}&lt;br /&gt;
 |&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Typical output:&#039;&#039;&#039; ~50 Gb for standard genomic DNA (sheared and size-selected); up to ~150 Gb for metagenomic or short-fragment libraries; theoretical maximum ~290 Gb per flowcell. Output is highly variable and depends on library quality and input DNA integrity. Read count is not guaranteed.&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Run duration:&#039;&#039;&#039; Up to 72 hours per flowcell (adjustable). Adaptive sampling and real-time basecalling occur during the run.&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Modified basecalling:&#039;&#039;&#039; Native DNA or RNA sequencing captures base modification information (5mC, 5hmC, 6mA, and others) without additional sample preparation. Not all modifications can be called in a single run with current chemistry; trace data (FAST5/POD5) can be re-basecalled with different models. Re-basecalling can be arranged as a separate bioinformatics project.&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Adaptive sampling:&#039;&#039;&#039; Enriches or depletes specific genomic regions in real time without physical selection steps. Users must supply at least a FASTA reference genome and optionally a BED file of target regions. Target regions should represent 0.1–10% of expected reads for enrichment or depletion to be effective.&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Contaminants:&#039;&#039;&#039; The following are known to damage or inhibit nanopores even at low concentrations and must be removed prior to submission:&lt;br /&gt;
* EDTA&lt;br /&gt;
* Alcohols (ethanol, isopropanol)&lt;br /&gt;
* High concentrations of salts (NaCl, guanidinium chloride, guanidinium isothiocyanate)&lt;br /&gt;
* Phenol&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
=== PromethION Library Preparation ===&lt;br /&gt;
&lt;br /&gt;
The BioMicro Center supports three standard library preparation kits for Nanopore sequencing. &lt;br /&gt;
&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; style=&amp;quot;width:100%; text-align:left;&amp;quot;&lt;br /&gt;
|-&lt;br /&gt;
! style=&amp;quot;background:#dce8f5; width:15%;&amp;quot; | Kit&lt;br /&gt;
! style=&amp;quot;background:#dce8f5; width:20%;&amp;quot; | Method&lt;br /&gt;
! style=&amp;quot;background:#dce8f5; width:65%;&amp;quot; | Details&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;LSK114&#039;&#039;&#039; &amp;lt;br&amp;gt; (SQK-LSK114)&lt;br /&gt;
| Ligation-based dsDNA&lt;br /&gt;
|&lt;br /&gt;
* &#039;&#039;&#039;Input:&#039;&#039;&#039; 1 µg clean dsDNA, &amp;gt;10 µL &amp;lt;BR&amp;gt;(50-100fmol)&lt;br /&gt;
* &#039;&#039;&#039;QC:&#039;&#039;&#039; UV-vis measurement; Final QC by Qubit&lt;br /&gt;
* &#039;&#039;&#039;Best for:&#039;&#039;&#039; Standard genomic DNA, flexible fragment sizes&lt;br /&gt;
* End repair → A-tail → adapter ligation → SPRI size selection&lt;br /&gt;
* Barcoding extension (SQK-NBD114) available for multiplexing up to 96 samples&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;RBK114&#039;&#039;&#039; &amp;lt;br&amp;gt; (SQK-RBK114)&lt;br /&gt;
| Tagmentation-based dsDNA&lt;br /&gt;
|&lt;br /&gt;
* &#039;&#039;&#039;Input:&#039;&#039;&#039; 400 ng clean dsDNA, &amp;gt;30 kbp preferred, &amp;gt;10 µL&lt;br /&gt;
* &#039;&#039;&#039;QC:&#039;&#039;&#039; UV-vis and FemtoPulse; Final QC by Qubit&lt;br /&gt;
* &#039;&#039;&#039;Best for:&#039;&#039;&#039; Ultra-long read applications; largest fragment sizes&lt;br /&gt;
* Tagmentation with adapters&lt;br /&gt;
* For ultra-long reads: microgram input, no size selection; reads &amp;gt;300 kbp observed but rare; overall yield is lower.&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;RNA004&#039;&#039;&#039; &amp;lt;br&amp;gt; (SQK-RNA004)&lt;br /&gt;
| Direct RNA sequencing&lt;br /&gt;
|&lt;br /&gt;
* &#039;&#039;&#039;Input:&#039;&#039;&#039; 1ug total RNA or 350 ng clean polyA+ mRNA (not total RNA), &amp;gt;10 µL&lt;br /&gt;
* &#039;&#039;&#039;QC:&#039;&#039;&#039; UV-vis and Fragment Analyzer; Final QC by Qubit&lt;br /&gt;
* &#039;&#039;&#039;Best for:&#039;&#039;&#039; Full-length transcript sequencing, isoform detection, RNA base modifications&lt;br /&gt;
* First-strand cDNA synthesis (optional but improves yield) → adapter ligation&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
&amp;lt;!-- ═══════════════════════════════ PACBIO REVIO ══════════════════════════ --&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== PacBio ==&lt;br /&gt;
&amp;lt;span id=&amp;quot;PACBIO&amp;quot;&amp;gt;&amp;lt;/span&amp;gt;&lt;br /&gt;
&lt;br /&gt;
PacBio Revio sequencing is available through BMC&#039;s collaboration with local academic core facilities. The Revio uses [https://www.pacb.com/technology/hifi-sequencing/ HiFi (CCS) sequencing]: a single SMRTbell library molecule is sequenced multiple times end-to-end in a rolling fashion, and the resulting subreads are assembled into a high-fidelity consensus read with very high accuracy. This error correction is built into the sequencing run itself — unlike Nanopore, which achieves high accuracy through consensus polishing after sequencing. Library preparation is performed at the BMC; sequencing is performed at a partner core.&lt;br /&gt;
&lt;br /&gt;
=== Revio — Sequencing ===&lt;br /&gt;
&lt;br /&gt;
{|&lt;br /&gt;
 |- style=&amp;quot;vertical-align:top;&amp;quot;&lt;br /&gt;
 | style=&amp;quot;width:370px;&amp;quot; |&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
 ! Service&lt;br /&gt;
 ! Revio Sequencing&lt;br /&gt;
 |-&lt;br /&gt;
 | INPUT || Completed SMRTbell libraries&lt;br /&gt;
 |-&lt;br /&gt;
 | MIN VOLUME || 12 µL&lt;br /&gt;
 |-&lt;br /&gt;
 | MIN CONCENTRATION || &amp;gt;1 nM &amp;lt;br&amp;gt; &#039;&#039;(1 nM ≈ 0.65 ng/µL/kb insert)&#039;&#039;&lt;br /&gt;
 |-&lt;br /&gt;
 | INCLUDED SERVICES&lt;br /&gt;
 |&lt;br /&gt;
* Sample QC on [[BioMicroCenter:QC#AATI_FEMTO_PULSE|FemtoPulse]]&lt;br /&gt;
* Sequencing&lt;br /&gt;
* Primary analysis (CCS/HiFi)&lt;br /&gt;
* Data storage for 1 year&lt;br /&gt;
 |-&lt;br /&gt;
 | ADDITIONAL SERVICES ||&lt;br /&gt;
* [[BioMicroCenter:PacBio_Library_Preparation|PacBio library preparation]]&lt;br /&gt;
* [[BioMicroCenter:PippinPrep|Pippin Prep]] for size selection&lt;br /&gt;
 |-&lt;br /&gt;
 | PRICING || [[MIT:Pricing]] · [[BioMicroCenter:Pricing]]&lt;br /&gt;
 |-&lt;br /&gt;
 | SUBMISSION&lt;br /&gt;
 |&lt;br /&gt;
* MIT – [https://mit.ilabsolutions.com/service_item/new/3381?spt_id=9912 iLabs]&lt;br /&gt;
* External – [[BioMicroCenter:Forms|form]]&lt;br /&gt;
 |-&lt;br /&gt;
 | PROVIDED THROUGH || Local academic core collaboration&lt;br /&gt;
 |-&lt;br /&gt;
|}&lt;br /&gt;
 |&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;How it works:&#039;&#039;&#039; The Revio loads ~10 M SMRTbell molecules into an array of zero-mode waveguides (ZMWs) by Poissonian distribution. Fluorescently labeled nucleotides are incorporated by a single polymerase tethered in each ZMW; incorporation events are detected in real time. Because the SMRTbell is circular, the polymerase reads the same molecule multiple times, generating subreads that are collapsed into a HiFi read with &amp;gt;&amp;gt;Q20 accuracy. Typical HiFi read lengths are 10–20 kb. &amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Output:&#039;&#039;&#039; ~80 Gb HiFi per SMRTcell. &amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Base modifications:&#039;&#039;&#039; Because the Revio measures polymerase kinetics (dwell time per base), it can detect base modifications that alter incorporation kinetics — including bacterial methylation (6mA, 4mC) and mammalian 5mC in CpG context. CpG methylation calling is included in standard HiFi analysis.&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
=== Revio — Library Preparation ===&lt;br /&gt;
&lt;br /&gt;
PacBio library preparation is performed at the BioMicro Center using the SMRTbell Prep Kit v3.0. Pricing includes quality control prior to prep.&lt;br /&gt;
&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; style=&amp;quot;width:100%; text-align:left;&amp;quot;&lt;br /&gt;
|-&lt;br /&gt;
! style=&amp;quot;background:#dce8f5; width:15%;&amp;quot; | Application&lt;br /&gt;
! style=&amp;quot;background:#dce8f5; width:20%;&amp;quot; | Method&lt;br /&gt;
! style=&amp;quot;background:#dce8f5; width:65%;&amp;quot; | Details&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;Genomic DNA&#039;&#039;&#039;&lt;br /&gt;
| SMRTbell Prep Kit v3.0&lt;br /&gt;
|&lt;br /&gt;
* &#039;&#039;&#039;Input:&#039;&#039;&#039; 150 ng–5 µg clean dsDNA, unsheared preferred at 50–60 kbp, &amp;gt;10 µL&lt;br /&gt;
* &#039;&#039;&#039;QC:&#039;&#039;&#039; UV-vis and FemtoPulse; Final QC by Qubit&lt;br /&gt;
* Single-strand overhang removal → damage repair → end repair → A-tailing → SMRTbell A/T ligation → two rounds of SPRI clean&lt;br /&gt;
* If average fragment size &amp;gt;50–60 kbp, Covaris gTube shearing available to target ~8-12 kbp&lt;br /&gt;
* Size selection verified by Fragment Analyzer or FemtoPulse&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
== PLATFORM COMPARISON ==&lt;br /&gt;
&amp;lt;span id=&amp;quot;PLATFORM_COMPARISON&amp;quot;&amp;gt;&amp;lt;/span&amp;gt;&lt;br /&gt;
&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; style=&amp;quot;width:100%; text-align:center;&amp;quot;&lt;br /&gt;
|-&lt;br /&gt;
! style=&amp;quot;background:#dce8f5;&amp;quot; | SPEC&lt;br /&gt;
! style=&amp;quot;background:#e6f4ea;&amp;quot; | ONT PromethION P2&lt;br /&gt;
! style=&amp;quot;background:#e8f0fe;&amp;quot; | PacBio Revio&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;Sequencing principle&#039;&#039;&#039;&lt;br /&gt;
| Ionic current through protein nanopore&lt;br /&gt;
| Real-time fluorescent SBS (ZMW array)&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;Read accuracy&#039;&#039;&#039;&lt;br /&gt;
| Raw reads: ~Q20; &amp;lt;br&amp;gt; consensus polished: &amp;gt;Q30 (99.9%)&lt;br /&gt;
| HiFi reads: ~Q40 on-instrument; &amp;lt;br&amp;gt; no post-run polishing required&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;Typical read length&#039;&#039;&#039;&lt;br /&gt;
| N50: 10–50 kb for standard gDNA; &amp;lt;br&amp;gt; ultra-long reads &amp;gt;100 kb achievable&lt;br /&gt;
| HiFi: 10-20 kb typical&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;Output / flowcell&#039;&#039;&#039;&lt;br /&gt;
| 50–150 Gb typical &amp;lt;br&amp;gt; (290 Gb theoretical max)&lt;br /&gt;
| ~80 Gb HiFi per SMRTcell&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;Run duration&#039;&#039;&#039;&lt;br /&gt;
| Up to 72 hours (adjustable)&lt;br /&gt;
| ~24 hours&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;Native base modification&#039;&#039;&#039;&lt;br /&gt;
| Yes — broad (5mC, 5hmC, 6mA, and more) &amp;lt;br&amp;gt; no additional prep required&lt;br /&gt;
| Yes — kinetics-based (6mA, 4mC, 5mC CpG) &amp;lt;br&amp;gt; included in standard HiFi analysis&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;Direct RNA sequencing&#039;&#039;&#039;&lt;br /&gt;
| Yes (SQK-RNA004)&lt;br /&gt;
| No&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;Adaptive sampling&#039;&#039;&#039;&lt;br /&gt;
| Yes — real-time enrichment/depletion&lt;br /&gt;
| No&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;Multiplexing&#039;&#039;&#039;&lt;br /&gt;
| Up to 96 barcodes/flowcell (NBD114)&lt;br /&gt;
| Limited — indexing practical mainly for amplicons&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;Instrument link&#039;&#039;&#039;&lt;br /&gt;
| [https://nanoporetech.com/products/sequence/promethion-2-solo PromethION P2 Solo]&lt;br /&gt;
| [https://www.pacb.com/revio/ PacBio Revio]&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
=== Strengths and best use cases ===&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Choose ONT PromethION when:&#039;&#039;&#039;&lt;br /&gt;
* Ultra-long reads are needed (&amp;gt;50 kb, e.g. spanning repeats or structural variants)&lt;br /&gt;
* Native base modification profiling beyond CpG (6mA, 5hmC, etc.)&lt;br /&gt;
* Direct RNA sequencing without cDNA conversion&lt;br /&gt;
* Adaptive sampling / real-time target enrichment or depletion&lt;br /&gt;
* High multiplexing (up to 96 samples/flowcell)&lt;br /&gt;
* Rapid turnaround is needed (real-time data during run)&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Choose PacBio Revio when:&#039;&#039;&#039;&lt;br /&gt;
* High single-read accuracy is required without post-run polishing&lt;br /&gt;
* Full-length isoform sequencing (IsoSeq) with highly accurate reads&lt;br /&gt;
* De novo assembly of moderate-complexity genomes with accurate long reads&lt;br /&gt;
* CpG methylation calling is needed alongside variant calling&lt;br /&gt;
&lt;br /&gt;
== FAQ ==&lt;br /&gt;
&amp;lt;span id=&amp;quot;FAQ&amp;quot;&amp;gt;&amp;lt;/span&amp;gt;&lt;br /&gt;
=== Can I get my base modification data? ===&lt;br /&gt;
Yes for both platforms. ONT detects a broad range of modifications natively; modified basecalling is available on request and can be applied to archived FAST5/POD5 data. PacBio CpG methylation calling is included in standard HiFi primary analysis.&lt;br /&gt;
&lt;br /&gt;
=== What is adaptive sampling and do I need to do anything special? ===&lt;br /&gt;
Adaptive sampling is an ONT-only feature that uses the sequencer to eject molecules in real time based on a reference sequence, enriching or depleting specific genomic regions without physical library manipulation. To use it, provide at least a FASTA reference genome (and optionally a BED file of target regions) at submission. Target regions should represent 0.1–10% of expected reads.&lt;br /&gt;
&lt;br /&gt;
=== What contaminants should I avoid for ONT? ===&lt;br /&gt;
EDTA, alcohols (ethanol, isopropanol), high-salt buffers (NaCl, guanidinium chloride, guanidinium isothiocyanate), and phenol will damage nanopores even at low concentrations and must be removed before submitting libraries. Contact [mailto:biomicro@mit.edu biomicro@mit.edu] if you are unsure about your elution buffer.&lt;br /&gt;
&lt;br /&gt;
== SUBMISSION ==&lt;br /&gt;
&lt;br /&gt;
All sequencing and library prep is submitted through [https://mit.ilabsolutions.com/service_item/new/3381?spt_id=3863 iLabs] (MIT users) or via the [[BioMicroCenter:Forms|external submission form]]. Contact [mailto:biomicro@mit.edu biomicro@mit.edu] before submitting non-standard samples or to discuss project design.&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:Sequencing&amp;diff=131386</id>
		<title>BioMicroCenter:Sequencing</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:Sequencing&amp;diff=131386"/>
		<updated>2026-04-21T00:52:02Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: Redirect to consolidated BioMicroCenter:ShortRead page&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;#REDIRECT [[BioMicroCenter:ShortRead]]&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:Singular_Sequencing&amp;diff=131385</id>
		<title>BioMicroCenter:Singular Sequencing</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:Singular_Sequencing&amp;diff=131385"/>
		<updated>2026-04-21T00:52:01Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: Redirect to consolidated BioMicroCenter:ShortRead page&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;#REDIRECT [[BioMicroCenter:ShortRead]]&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:Element_Sequencing&amp;diff=131384</id>
		<title>BioMicroCenter:Element Sequencing</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:Element_Sequencing&amp;diff=131384"/>
		<updated>2026-04-21T00:52:00Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: Redirect to consolidated BioMicroCenter:ShortRead page&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;#REDIRECT [[BioMicroCenter:ShortRead]]&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:Illumina_Sequencing&amp;diff=131383</id>
		<title>BioMicroCenter:Illumina Sequencing</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:Illumina_Sequencing&amp;diff=131383"/>
		<updated>2026-04-21T00:51:59Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: Redirect to consolidated BioMicroCenter:ShortRead page&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;#REDIRECT [[BioMicroCenter:ShortRead]]&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:ShortRead&amp;diff=131382</id>
		<title>BioMicroCenter:ShortRead</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:ShortRead&amp;diff=131382"/>
		<updated>2026-04-21T00:00:00Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: Revise platform detail sections: add manufacturer intros, run times, custom oligo requirements, external links&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;{{BioMicroCenter}}&lt;br /&gt;
&lt;br /&gt;
The MIT BioMicro Center offers short-read sequencing on five platforms from three manufacturers, spanning low to high output. All sequencing includes library quality control (RT-PCR and Fragment Analyzer), demultiplexing, and FASTQ delivery. Pricing is available to MIT users at [[MIT:Pricing]] and to all users at [[BioMicroCenter:Pricing]].&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;background:#f8f9fa; border:1px solid #ccc; padding:12px 16px; margin:12px 0;&amp;quot;&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;Choosing a platform?&#039;&#039;&#039; Jump to [[#PLATFORM_COMPARISON|platform comparison]] and [[#FAQ|FAQ]] below, or contact [mailto:biomicro@mit.edu biomicro@mit.edu].&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== OUTPUT TIERS ==&lt;br /&gt;
&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; style=&amp;quot;width:100%; text-align:center;&amp;quot;&lt;br /&gt;
|-&lt;br /&gt;
! style=&amp;quot;width:8%; background:#dce8f5;&amp;quot; | OUTPUT&lt;br /&gt;
! style=&amp;quot;width:18%; background:#dce8f5;&amp;quot; | PLATFORM&lt;br /&gt;
! style=&amp;quot;width:22%; background:#dce8f5;&amp;quot; | READS / UNIT&lt;br /&gt;
! style=&amp;quot;width:20%; background:#dce8f5;&amp;quot; | MIN SUBMISSION&lt;br /&gt;
! style=&amp;quot;width:32%; background:#dce8f5;&amp;quot; | TYPICAL USE&lt;br /&gt;
|-&lt;br /&gt;
| style=&amp;quot;background:#e8f0fe; font-weight:bold; vertical-align:middle;&amp;quot; rowspan=&amp;quot;2&amp;quot; | HIGH&lt;br /&gt;
| [[Image:BMC_NovaXplus.jpg|center|120px]] &amp;lt;br&amp;gt; &#039;&#039;&#039;NovaSeqX Plus&#039;&#039;&#039;&lt;br /&gt;
| 1.2B reads/lane (10B FC) &amp;lt;br&amp;gt; 3.0B reads/lane (25B FC)&lt;br /&gt;
| 110 µL at 2 nM per lane&lt;br /&gt;
| rowspan=&amp;quot;2&amp;quot; style=&amp;quot;text-align:left; font-size:92%;&amp;quot; | Largest experiments requiring billions of reads: cohort-scale WGS, ultra-deep sequencing, high-replicate RNA-seq studies&lt;br /&gt;
|-&lt;br /&gt;
| colspan=&amp;quot;3&amp;quot; style=&amp;quot;font-size:90%; color:#555; text-align:left; padding:4px 10px;&amp;quot; | &#039;&#039;Available through collaboration with nearby academic shared resources. Contact [mailto:biomicro@mit.edu BMC staff] to inquire.&#039;&#039;&lt;br /&gt;
|-&lt;br /&gt;
| style=&amp;quot;background:#e6f4ea; font-weight:bold; vertical-align:middle;&amp;quot; | MID&lt;br /&gt;
| [[Image:Element_AVITI.jpg|center|120px]] &amp;lt;br&amp;gt; &#039;&#039;&#039;Element AVITI24&#039;&#039;&#039;&lt;br /&gt;
| ~400M reads/lane &amp;lt;br&amp;gt; (~800M per 2-lane flowcell)&lt;br /&gt;
| 15 µL at 2 nM&lt;br /&gt;
| style=&amp;quot;text-align:left; font-size:92%;&amp;quot; | Standard depth experiments: RNA-seq, ChIP-seq, ATAC-seq, WGS at moderate coverage; tolerates low-complexity libraries well&lt;br /&gt;
|-&lt;br /&gt;
| style=&amp;quot;background:#fff3e0; font-weight:bold; vertical-align:middle;&amp;quot; rowspan=&amp;quot;4&amp;quot; | LOW&lt;br /&gt;
| [[Image:2023_Singular_G4.jpg|center|120px]] &amp;lt;br&amp;gt; &#039;&#039;&#039;Singular G4&#039;&#039;&#039;&lt;br /&gt;
| ~100M reads/lane &amp;lt;br&amp;gt; (4 lanes/flowcell)&lt;br /&gt;
| 12 µL at 4 nM&lt;br /&gt;
| rowspan=&amp;quot;4&amp;quot; style=&amp;quot;text-align:left; font-size:92%;&amp;quot; | Smaller experiments, amplicons, validation, pilot studies, and applications where cost-per-lane is the primary driver&lt;br /&gt;
|-&lt;br /&gt;
| [[Image:BMC_i100.jpg|center|120px]] &amp;lt;br&amp;gt; &#039;&#039;&#039;MiSeq i100&#039;&#039;&#039;&lt;br /&gt;
| 5M – 100M reads/flowcell&lt;br /&gt;
| 40 µL at 2 nM&lt;br /&gt;
|-&lt;br /&gt;
| &amp;lt;!-- no image yet: replace with [[Image:filename.ext|center|120px]] --&amp;gt; &amp;lt;br&amp;gt; &#039;&#039;&#039;MiSeq classic&#039;&#039;&#039;&lt;br /&gt;
| 1M – 25M reads/flowcell&lt;br /&gt;
| 40 µL at 2 nM&lt;br /&gt;
|-&lt;br /&gt;
| colspan=&amp;quot;3&amp;quot; style=&amp;quot;font-size:90%; color:#555; text-align:left; padding:4px 10px;&amp;quot; | &#039;&#039;MiSeq classic is off contract and will be retired upon instrument failure. MiSeq i100 offers walkup service (MIT only, training required).&#039;&#039;&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
== SEQUENCING PLATFORMS ==&lt;br /&gt;
&lt;br /&gt;
&amp;lt;!-- ═══════════════════════════════ ILLUMINA ═══════════════════════════════ --&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Illumina ==&lt;br /&gt;
&amp;lt;span id=&amp;quot;ILLUMINA&amp;quot;&amp;gt;&amp;lt;/span&amp;gt;&lt;br /&gt;
&lt;br /&gt;
The BioMicro Center operates the [https://www.illumina.com/systems/sequencing-platforms/miseq-i100.html MiSeq i100] and provides access to [https://www.illumina.com/systems/sequencing-platforms/novaseq-x-plus.html NovaSeqX Plus] through collaboration with nearby academic core facilities. Both instruments use Illumina&#039;s two-color XLEAP-SBS chemistry (G bases are inferred from signal absence) and are best suited to high-complexity libraries. UDIs are strongly recommended on both platforms.&lt;br /&gt;
&lt;br /&gt;
=== NovaSeqX Plus ===&lt;br /&gt;
&lt;br /&gt;
NovaSeqX Plus sequencing is available through BMC&#039;s collaboration with local academic core facilities, and is well suited to projects requiring the deepest coverage or largest sample counts. It is only available for MIT users. These collaborations provide advantaged pricing to MIT users.&lt;br /&gt;
&lt;br /&gt;
* See [[MIT:Pricing|Pricing]] for rates.&lt;br /&gt;
* Full flowcells (8 lanes, 10B or 25B) have priority over individual lanes; wait times for single lanes can vary.&lt;br /&gt;
* Minimum 2 nM; 110 µL per lane.&lt;br /&gt;
* Custom sequencing primers: at least 30 µL at 100 µM of each primer type per flowcell. Contact [mailto:biomicro@mit.edu biomicro@mit.edu] before planning a custom library prep.&lt;br /&gt;
&lt;br /&gt;
=== MiSeq i100 ===&lt;br /&gt;
&lt;br /&gt;
{|&lt;br /&gt;
 |- style=&amp;quot;vertical-align:top;&amp;quot;&lt;br /&gt;
 | style=&amp;quot;width:370px;&amp;quot; |&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
 ! Service&lt;br /&gt;
 ! MiSeq i100 Sequencing&lt;br /&gt;
 |-&lt;br /&gt;
 | INPUT || Illumina libraries&lt;br /&gt;
 |-&lt;br /&gt;
 | MIN VOLUME || 40 µL&lt;br /&gt;
 |-&lt;br /&gt;
 | MIN CONCENTRATION || 2 nM (~0.4 ng/µL for 300 bp library)&lt;br /&gt;
 |-&lt;br /&gt;
 | INCLUDED SERVICES&lt;br /&gt;
 |&lt;br /&gt;
* Quality Control: Fragment Analyzer and qPCR&lt;br /&gt;
* Illumina Sequencing&lt;br /&gt;
* Demultiplexing&lt;br /&gt;
 |-&lt;br /&gt;
 | DATA FORMATS&lt;br /&gt;
 |&lt;br /&gt;
* FASTQ&lt;br /&gt;
* BCL (stored 30 days)&lt;br /&gt;
 |-&lt;br /&gt;
 | PRICING || [[MIT:Pricing]] · [[BioMicroCenter:Pricing]]&lt;br /&gt;
 |-&lt;br /&gt;
 | SUBMISSION&lt;br /&gt;
 |&lt;br /&gt;
* MIT (Assisted) – [https://mit.ilabsolutions.com/service_item/new/3381?spt_id=3863 iLabs]&lt;br /&gt;
* MIT (Walkup) – [https://mit.ilabsolutions.com/schedules/555535#/schedule/ iLabs calendar] (training required)&lt;br /&gt;
 |-&lt;br /&gt;
|}&lt;br /&gt;
 |&lt;br /&gt;
&lt;br /&gt;
The MiSeq i100 uses XLEAP-SBS chemistry on a patterned (CMOS nanowell) flowcell and is the BMC&#039;s walkup-capable instrument. It supports the longest read lengths of any instrument in the center (up to 1000 nt), making it ideal for amplicon panels, 16S rRNA surveys, validation runs, and other applications where read length matters more than throughput.&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Kits available:&#039;&#039;&#039; 100 nt (25M, 100M), 300 nt (5M, 25M, 100M), 600 nt (5M, 25M, 50M), 1000 nt (25M). Run times range from under 4 hours (25M 100 nt) to ~24 hours (100M 300 nt).&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Walkup service&#039;&#039;&#039; is not available to external users. Runs must be scheduled in the [https://mit.ilabsolutions.com/schedules/555535#/schedule/ iLabs calendar]. Server training and storage access on BMC servers is required.&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Custom sequencing primers:&#039;&#039;&#039; At least 15 µL of each primer type at 100 µM must be provided per flowcell. Contact [mailto:biomicro@mit.edu biomicro@mit.edu] before planning a custom library prep.&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Thanks to:&#039;&#039;&#039; MIT VPR, Drs. Gene Li, Michael Birnbaum, Dept. of Biology, Scott Ritterbush &#039;89 SM &#039;92&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
=== MiSeq classic ===&lt;br /&gt;
&lt;br /&gt;
The MiSeq classic uses 4-color SBS chemistry on a non-patterned (random cluster) flowcell. It is available for users with established protocols on v2/v3 chemistry. We maintain minimal reagent stocks so turn around times may vary.&lt;br /&gt;
&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
 ! Kit&lt;br /&gt;
 ! Approx. reads&lt;br /&gt;
 ! Notes&lt;br /&gt;
 |-&lt;br /&gt;
 | 70 nt (V2) || ~15M || 30 or 70 SE&lt;br /&gt;
 |-&lt;br /&gt;
 | 150 nt (V3) || ~25M || 75 PE&lt;br /&gt;
 |-&lt;br /&gt;
 | 300 nt (V2) || ~15M || 150 PE&lt;br /&gt;
 |-&lt;br /&gt;
 | 500 nt (V2) || ~15M || 250 PE&lt;br /&gt;
 |-&lt;br /&gt;
 | 600 nt (V3) || ~25M || 300 PE&lt;br /&gt;
 |-&lt;br /&gt;
 | 500 nt NANO || ~1M || Reduced output run&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
See [[MIT:Pricing]] · [[BioMicroCenter:Pricing]] for kit pricing. Custom sequencing primers follow the same submission guidelines as the MiSeq i100: at least 15 µL at 100 µM per primer type.&lt;br /&gt;
&lt;br /&gt;
&amp;lt;!-- Donor information for MiSeq classic is not recorded in current source pages. Please update if known. --&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;!-- ═══════════════════════════ ELEMENT BIOSCIENCES ═══════════════════════ --&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Element Biosciences ==&lt;br /&gt;
&amp;lt;span id=&amp;quot;ELEMENT&amp;quot;&amp;gt;&amp;lt;/span&amp;gt;&lt;br /&gt;
&lt;br /&gt;
The BioMicro Center operates an [https://www.elementbiosciences.com/products/aviti24 AVITI24] from Element Biosciences. The AVITI24 uses Element&#039;s proprietary [https://www.elementbiosciences.com/resources/avidity-sequencing Avidity Base Chemistry (ABC)], a 4-color sequencing approach that achieves high base-call accuracy (&amp;gt;80% of reads Q40+) by leveraging multivalent binding of avidites rather than single-nucleotide incorporation. The random flowcell and rolling-circle amplification approach produce low levels of index hopping and optical duplicates compared to patterned-flowcell instruments. Beyond standard NGS, the AVITI24 supports [https://www.elementbiosciences.com/products/aviti24/cytoprofiling Teton CytoProfiling] for direct in-cell co-detection of RNA, protein, and morphology — see [[BioMicroCenter:Spatial|Spatial Services]] for details.&lt;br /&gt;
&lt;br /&gt;
=== AVITI24 ===&lt;br /&gt;
&lt;br /&gt;
{|&lt;br /&gt;
 |- style=&amp;quot;vertical-align:top;&amp;quot;&lt;br /&gt;
 | style=&amp;quot;width:370px;&amp;quot; |&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
 ! Service&lt;br /&gt;
 ! Element Sequencing&lt;br /&gt;
 |-&lt;br /&gt;
 | SUBMISSION || Illumina (P5/P7) libraries&lt;br /&gt;
 |-&lt;br /&gt;
 | MIN VOLUME || 15 µL&lt;br /&gt;
 |-&lt;br /&gt;
 | MIN CONCENTRATION || 2 nM&lt;br /&gt;
 |-&lt;br /&gt;
 | INCLUDED SERVICES&lt;br /&gt;
 |&lt;br /&gt;
* Quality Control: Fragment Analyzer and qPCR&lt;br /&gt;
* Element Sequencing&lt;br /&gt;
* Demultiplexing&lt;br /&gt;
 |-&lt;br /&gt;
 | ADDITIONAL SERVICES ||&lt;br /&gt;
* Quality Control&lt;br /&gt;
* Element library preparation&lt;br /&gt;
 |-&lt;br /&gt;
 | DATA FORMATS&lt;br /&gt;
 |&lt;br /&gt;
* FASTQ (stored 1 year)&lt;br /&gt;
 |-&lt;br /&gt;
 | QUALITY CONTROL&lt;br /&gt;
 |&lt;br /&gt;
* [https://www.bioinformatics.babraham.ac.uk/projects/fastqc/ FASTQC]&lt;br /&gt;
* Basic run metrics (alignment rate, complexity)&lt;br /&gt;
* Basic RNAseq metrics (where applicable)&lt;br /&gt;
* Basic paired end metrics (where applicable)&lt;br /&gt;
* Contamination checks&lt;br /&gt;
 |-&lt;br /&gt;
 | PRICING || [[MIT:Pricing]] · [[BioMicroCenter:Pricing]]&lt;br /&gt;
 |-&lt;br /&gt;
 | SUBMISSION&lt;br /&gt;
 |&lt;br /&gt;
* MIT – [https://mit.ilabsolutions.com/service_item/new/3381?spt_id=3863 iLabs]&lt;br /&gt;
 |-&lt;br /&gt;
|}&lt;br /&gt;
 |&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Kits and run times:&#039;&#039;&#039;&lt;br /&gt;
* 75 PE (150 nt total): ~24 hours, ~400M reads/lane&lt;br /&gt;
* 150 PE (300 nt total): ~38 hours, ~400M reads/lane&lt;br /&gt;
* 300 PE (600 nt total): ~60 hours, ~200M reads (single lane only)&lt;br /&gt;
&lt;br /&gt;
Each flowcell has two independently-addressable lanes. Both lanes must be loaded to run a flowcell, but each can carry a different library pool. 300PE kits are single-lane only.&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Anchors:&#039;&#039;&#039; The BMC runs P5/P7-anchored (Illumina Freestyle) libraries as standard. Element Adept (SP5/SP27) libraries are also supported but require a 5′-phosphate on the forward strand for circularization — contact [mailto:biomicro@mit.edu biomicro@mit.edu] before preparing Adept libraries.&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Indexing:&#039;&#039;&#039; Index orientation differs from standard Illumina: indexes are read from antisense primers with the P7-side first (index 1). Provide index sequences &#039;&#039;&#039;as read&#039;&#039;&#039; on the instrument, not as ordered. For dual-indexed libraries this is typically antisense to the ordered oligo sequence.&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Custom primers:&#039;&#039;&#039; 70 µL at 100 µM for Read 1; 40 µL at 100 µM for all other reads. Custom primers must not interfere with other users&#039; samples in the same lane.&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Minimum read guarantee:&#039;&#039;&#039; Guaranteed if (a) BMC performed QC, (b) library diversity is high especially in the first 5 bases of Read 1, and (c) concentration ≥ 2 nM.&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Thanks to:&#039;&#039;&#039; Element Biosciences; Scott Ritterbush&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
&amp;lt;!-- ═══════════════════════════ SINGULAR GENOMICS ════════════════════════ --&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Singular Genomics ==&lt;br /&gt;
&amp;lt;span id=&amp;quot;SINGULAR&amp;quot;&amp;gt;&amp;lt;/span&amp;gt;&lt;br /&gt;
&lt;br /&gt;
The BioMicro Center operates a [https://www.singulargenomics.com G4] from Singular Genomics. The G4 uses a 4-color SBS chemistry on a patterned flowcell and delivers index-hopping rates comparable to non-patterned Illumina instruments (HiSeq/GAIIx level) — substantially lower than X-Amp patterned flowcells. This makes it a practical choice for experiments that do not require UDIs. The G4 supports four independently-addressable lanes per flowcell and up to four flowcells per run, offering per-lane flexibility at competitive per-lane cost.&lt;br /&gt;
&lt;br /&gt;
=== G4 ===&lt;br /&gt;
&lt;br /&gt;
{|&lt;br /&gt;
 |- style=&amp;quot;vertical-align:top;&amp;quot;&lt;br /&gt;
 | style=&amp;quot;width:370px;&amp;quot; |&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
 ! Service&lt;br /&gt;
 ! Singular G4 Sequencing&lt;br /&gt;
 |-&lt;br /&gt;
 | INPUT || Illumina (P5/P7) libraries&lt;br /&gt;
 |-&lt;br /&gt;
 | MIN VOLUME || 12 µL&lt;br /&gt;
 |-&lt;br /&gt;
 | MIN CONCENTRATION || 4 nM&lt;br /&gt;
 |-&lt;br /&gt;
 | INCLUDED SERVICES&lt;br /&gt;
 |&lt;br /&gt;
* Quality Control: Fragment Analyzer and qPCR&lt;br /&gt;
* Pool conversion: P5/P7 → S1/S2 by PCR&lt;br /&gt;
* Singular Sequencing&lt;br /&gt;
* Demultiplexing&lt;br /&gt;
 |-&lt;br /&gt;
 | ADDITIONAL SERVICES ||&lt;br /&gt;
* Quality Control&lt;br /&gt;
* Singular library preparation&lt;br /&gt;
 |-&lt;br /&gt;
 | DATA FORMATS&lt;br /&gt;
 |&lt;br /&gt;
* FASTQ (stored 1 year)&lt;br /&gt;
 |-&lt;br /&gt;
 | QUALITY CONTROL&lt;br /&gt;
 |&lt;br /&gt;
* [https://www.bioinformatics.babraham.ac.uk/projects/fastqc/ FASTQC]&lt;br /&gt;
* Basic run metrics (alignment rate, complexity)&lt;br /&gt;
* Basic RNAseq metrics (where applicable)&lt;br /&gt;
* Contamination checks&lt;br /&gt;
 |-&lt;br /&gt;
 | PRICING || [[MIT:Pricing]] · [[BioMicroCenter:Pricing]]&lt;br /&gt;
 |-&lt;br /&gt;
 | SUBMISSION&lt;br /&gt;
 |&lt;br /&gt;
* MIT – [https://mit.ilabsolutions.com/service_item/new/3381?spt_id=3863 iLabs]&lt;br /&gt;
 |-&lt;br /&gt;
|}&lt;br /&gt;
 |&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Kits and run times:&#039;&#039;&#039;&lt;br /&gt;
* 50 PE (100 nt total): ~11 hours, ~100M reads/lane&lt;br /&gt;
* 150 PE (300 nt total): ~24 hours, ~100M reads/lane&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Library submission:&#039;&#039;&#039; Submit standard Illumina (P5/P7) libraries. The BMC converts the pool to S1/S2 anchors by PCR prior to sequencing — do &#039;&#039;&#039;not&#039;&#039;&#039; submit S1/S2-anchored libraries directly.&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Indexing:&#039;&#039;&#039; After conversion, indexes are read from S1 (index 1) then S2 (index 2), in the &#039;&#039;&#039;same orientation&#039;&#039;&#039; as ordered in classical Illumina library preps. Dual 8-nt indexes are required for all lane-by-lane sequencing. Custom index primers are not permitted.&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Lane by lane:&#039;&#039;&#039;&lt;br /&gt;
* Must be 50 PE or 150 PE dual-indexed.&lt;br /&gt;
* Pool must be compatible with TruSeq, Nextera, smRNA, or Solexa sequencing primers.&lt;br /&gt;
* 4 lanes per flowcell; up to 4 flowcells per run.&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Minimum read guarantee:&#039;&#039;&#039; Guaranteed if (a) BMC performed QC, (b) no custom primers, and (c) concentration ≥ 4 nM.&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Thanks to:&#039;&#039;&#039; Singular Genomics&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
== PLATFORM COMPARISON ==&lt;br /&gt;
&amp;lt;span id=&amp;quot;PLATFORM_COMPARISON&amp;quot;&amp;gt;&amp;lt;/span&amp;gt;&lt;br /&gt;
&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; style=&amp;quot;width:100%; text-align:center;&amp;quot;&lt;br /&gt;
|-&lt;br /&gt;
! style=&amp;quot;background:#dce8f5;&amp;quot; | SPEC&lt;br /&gt;
! style=&amp;quot;background:#e8f0fe;&amp;quot; | NovaSeqX Plus&lt;br /&gt;
! style=&amp;quot;background:#e6f4ea;&amp;quot; | Element AVITI24&lt;br /&gt;
! style=&amp;quot;background:#fff3e0;&amp;quot; | Singular G4&lt;br /&gt;
! style=&amp;quot;background:#fff3e0;&amp;quot; | MiSeq i100&lt;br /&gt;
! style=&amp;quot;background:#fff3e0;&amp;quot; | MiSeq classic&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;Read lengths&#039;&#039;&#039;&lt;br /&gt;
| 100 nt, 300 nt&lt;br /&gt;
| 75 PE, 150 PE, 300 PE&lt;br /&gt;
| 50 PE, 150 PE&lt;br /&gt;
| 100–1000 nt&lt;br /&gt;
| 70–600 nt&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;Flowcell type&#039;&#039;&#039;&lt;br /&gt;
| Patterned (X-Amp)&lt;br /&gt;
| Random&lt;br /&gt;
| Patterned&lt;br /&gt;
| Patterned (CMOS nanowell)&lt;br /&gt;
| Random&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;Chemistry&#039;&#039;&#039;&lt;br /&gt;
| 2-color XLEAP-SBS (G=dark)&lt;br /&gt;
| 4-color avidity SBS&lt;br /&gt;
| 4-color SBS&lt;br /&gt;
| 2-color XLEAP-SBS (G=dark)&lt;br /&gt;
| 4-color SBS&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;Index hopping&#039;&#039;&#039;&lt;br /&gt;
| High — UDIs required&lt;br /&gt;
| Low — UDIs not needed&lt;br /&gt;
| Low (patterned, non-XLEAP)&lt;br /&gt;
| Present — UDIs recommended&lt;br /&gt;
| Low (non-patterned)&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;Low-complexity libraries&#039;&#039;&#039;&lt;br /&gt;
| Struggles&lt;br /&gt;
| Handles well&lt;br /&gt;
| Handles well&lt;br /&gt;
| Struggles&lt;br /&gt;
| Handles well&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;Lane flexibility&#039;&#039;&#039;&lt;br /&gt;
| Lane-by-lane (full FC priority)&lt;br /&gt;
| Lane-by-lane&lt;br /&gt;
| Lane-by-lane&lt;br /&gt;
| Full flowcell only&lt;br /&gt;
| Full flowcell only&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;Walkup&#039;&#039;&#039;&lt;br /&gt;
| No (collaboration)&lt;br /&gt;
| No&lt;br /&gt;
| No&lt;br /&gt;
| Yes (training required)&lt;br /&gt;
| No&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;Illumina lib compatible&#039;&#039;&#039;&lt;br /&gt;
| Yes (native)&lt;br /&gt;
| Yes (P5/P7 Freestyle)&lt;br /&gt;
| Yes — BMC converts pool by PCR&lt;br /&gt;
| Yes (native)&lt;br /&gt;
| Yes (native)&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;Donated / supported by&#039;&#039;&#039;&lt;br /&gt;
| Local core collaboration&lt;br /&gt;
| Element Biosciences; Scott Ritterbush&lt;br /&gt;
| Singular Genomics&lt;br /&gt;
| MIT VPR; Drs. Gene Li, Michael Birnbaum; Dept. of Biology; Scott Ritterbush &#039;89 SM &#039;92&lt;br /&gt;
| &amp;lt;!-- unknown: please update --&amp;gt;&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
== FAQ ==&lt;br /&gt;
&amp;lt;span id=&amp;quot;FAQ&amp;quot;&amp;gt;&amp;lt;/span&amp;gt;&lt;br /&gt;
&lt;br /&gt;
=== My library has low complexity. Which platform should I use? ===&lt;br /&gt;
Avoid the NovaSeqX Plus and MiSeq i100 — both use 2-color XLEAP-SBS chemistry where G bases are inferred from signal absence, making low-diversity early cycles prone to run failure. The AVITI24, Singular G4, and MiSeq classic all use 4-color chemistry and handle low-complexity libraries well. Always contact [mailto:biomicro@mit.edu biomicro@mit.edu] before submitting unconventional libraries.&lt;br /&gt;
&lt;br /&gt;
=== Do I need unique dual indexes (UDIs)? ===&lt;br /&gt;
* &#039;&#039;&#039;NovaSeqX Plus&#039;&#039;&#039; – Yes, required (patterned X-Amp flowcell).&lt;br /&gt;
* &#039;&#039;&#039;MiSeq i100&#039;&#039;&#039; – Recommended (patterned CMOS flowcell).&lt;br /&gt;
* &#039;&#039;&#039;AVITI24&#039;&#039;&#039; – Not required; index hopping is low on random flowcells.&lt;br /&gt;
* &#039;&#039;&#039;Singular G4&#039;&#039;&#039; – Not required; patterned but does not use X-Amp chemistry; hopping is on par with HiSeq/GAIIx.&lt;br /&gt;
* &#039;&#039;&#039;MiSeq classic&#039;&#039;&#039; – Not required; non-patterned flowcell has low hopping.&lt;br /&gt;
&lt;br /&gt;
=== How long is my data stored? ===&lt;br /&gt;
* FASTQ files: 1 year for AVITI24, Singular G4, MiSeq i100.&lt;br /&gt;
* BCL files (MiSeq): 30 days.&lt;br /&gt;
* Long-term storage is available; see [[BioMicroCenter:Pricing]] for active storage and tape archive options.&lt;br /&gt;
&lt;br /&gt;
=== What quality control is included? ===&lt;br /&gt;
All runs include a final pool QC (RT-PCR and Fragment Analyzer) and post-sequencing QC (FastQC, run metrics, alignment rate, complexity, RNAseq metrics where applicable, contamination checks). Users who opt out of pre-sequencing QC by providing their own concentration forfeit read guarantees.&lt;br /&gt;
&lt;br /&gt;
=== Can I pool samples from multiple labs? ===&lt;br /&gt;
Yes, but users are responsible for minimizing index cross-contamination and [https://www.illumina.com/science/education/minimizing-index-hopping.html index crosstalk]. The BMC does not combine projects within a lane.&lt;br /&gt;
&lt;br /&gt;
== SUBMISSION ==&lt;br /&gt;
&lt;br /&gt;
All sequencing is submitted through [https://mit.ilabsolutions.com/service_item/new/3381?spt_id=3863 iLabs]. External users should contact [mailto:biomicro@mit.edu biomicro@mit.edu] directly. Samples must meet the minimum volume and concentration requirements listed above; coordinate with BMC staff before sending non-standard submissions.&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:ShortRead&amp;diff=131375</id>
		<title>BioMicroCenter:ShortRead</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:ShortRead&amp;diff=131375"/>
		<updated>2026-04-20T23:28:44Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: Create consolidated short read sequencing page (Illumina, Element, Singular)&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;{{BioMicroCenter}}&lt;br /&gt;
&lt;br /&gt;
The MIT BioMicro Center offers short-read sequencing on five platforms from three manufacturers, spanning low to high output. All sequencing includes library quality control (RT-PCR and Fragment Analyzer), demultiplexing, and FASTQ delivery. Pricing is available to MIT users at [[MIT:Pricing]] and to all users at [[BioMicroCenter:Pricing]].&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;background:#f8f9fa; border:1px solid #ccc; padding:12px 16px; margin:12px 0;&amp;quot;&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;Choosing a platform?&#039;&#039;&#039; Jump to [[#PLATFORM_COMPARISON|platform comparison]] and [[#FAQ|FAQ]] below, or contact [mailto:biomicro@mit.edu biomicro@mit.edu].&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== OUTPUT TIERS ==&lt;br /&gt;
&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; style=&amp;quot;width:100%; text-align:center;&amp;quot;&lt;br /&gt;
|-&lt;br /&gt;
! style=&amp;quot;width:8%; background:#dce8f5;&amp;quot; | OUTPUT&lt;br /&gt;
! style=&amp;quot;width:18%; background:#dce8f5;&amp;quot; | PLATFORM&lt;br /&gt;
! style=&amp;quot;width:22%; background:#dce8f5;&amp;quot; | READS / UNIT&lt;br /&gt;
! style=&amp;quot;width:20%; background:#dce8f5;&amp;quot; | MIN SUBMISSION&lt;br /&gt;
! style=&amp;quot;width:32%; background:#dce8f5;&amp;quot; | TYPICAL USE&lt;br /&gt;
|-&lt;br /&gt;
| style=&amp;quot;background:#e8f0fe; font-weight:bold; vertical-align:middle;&amp;quot; rowspan=&amp;quot;2&amp;quot; | HIGH&lt;br /&gt;
| [[Image:BMC_NovaXplus.jpg|center|120px]] &amp;lt;br&amp;gt; &#039;&#039;&#039;NovaSeqX Plus&#039;&#039;&#039;&lt;br /&gt;
| 1.2B reads/lane (10B FC) &amp;lt;br&amp;gt; 3.0B reads/lane (25B FC)&lt;br /&gt;
| 110 µL at 2 nM per lane&lt;br /&gt;
| rowspan=&amp;quot;2&amp;quot; style=&amp;quot;text-align:left; font-size:92%;&amp;quot; | Largest experiments requiring billions of reads: cohort-scale WGS, ultra-deep sequencing, high-replicate RNA-seq studies&lt;br /&gt;
|-&lt;br /&gt;
| colspan=&amp;quot;3&amp;quot; style=&amp;quot;font-size:90%; color:#555; text-align:left; padding:4px 10px;&amp;quot; | &#039;&#039;Available through collaboration with nearby academic shared resources. Contact [mailto:biomicro@mit.edu BMC staff] to inquire.&#039;&#039;&lt;br /&gt;
|-&lt;br /&gt;
| style=&amp;quot;background:#e6f4ea; font-weight:bold; vertical-align:middle;&amp;quot; | MID&lt;br /&gt;
| [[Image:Element_AVITI.jpg|center|120px]] &amp;lt;br&amp;gt; &#039;&#039;&#039;Element AVITI24&#039;&#039;&#039;&lt;br /&gt;
| ~400M reads/lane &amp;lt;br&amp;gt; (~800M per 2-lane flowcell)&lt;br /&gt;
| 15 µL at 2 nM&lt;br /&gt;
| style=&amp;quot;text-align:left; font-size:92%;&amp;quot; | Standard depth experiments: RNA-seq, ChIP-seq, ATAC-seq, WGS at moderate coverage; tolerates low-complexity libraries well&lt;br /&gt;
|-&lt;br /&gt;
| style=&amp;quot;background:#fff3e0; font-weight:bold; vertical-align:middle;&amp;quot; rowspan=&amp;quot;4&amp;quot; | LOW&lt;br /&gt;
| [[Image:2023_Singular_G4.jpg|center|120px]] &amp;lt;br&amp;gt; &#039;&#039;&#039;Singular G4&#039;&#039;&#039;&lt;br /&gt;
| ~100M reads/lane &amp;lt;br&amp;gt; (4 lanes/flowcell)&lt;br /&gt;
| 12 µL at 4 nM&lt;br /&gt;
| rowspan=&amp;quot;4&amp;quot; style=&amp;quot;text-align:left; font-size:92%;&amp;quot; | Smaller experiments, amplicons, validation, pilot studies, and applications where cost-per-lane is the primary driver&lt;br /&gt;
|-&lt;br /&gt;
| [[Image:BMC_i100.jpg|center|120px]] &amp;lt;br&amp;gt; &#039;&#039;&#039;MiSeq i100&#039;&#039;&#039;&lt;br /&gt;
| 5M – 100M reads/flowcell&lt;br /&gt;
| 40 µL at 2 nM&lt;br /&gt;
|-&lt;br /&gt;
| &amp;lt;!-- no image yet: replace with [[Image:filename.ext|center|120px]] --&amp;gt; &amp;lt;br&amp;gt; &#039;&#039;&#039;MiSeq classic&#039;&#039;&#039;&lt;br /&gt;
| 5M – 25M reads/flowcell&lt;br /&gt;
| 40 µL at 2 nM&lt;br /&gt;
|-&lt;br /&gt;
| colspan=&amp;quot;3&amp;quot; style=&amp;quot;font-size:90%; color:#555; text-align:left; padding:4px 10px;&amp;quot; | &#039;&#039;MiSeq classic is off contract and will be retired upon instrument failure. MiSeq i100 offers walkup service (MIT only, training required).&#039;&#039;&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
== SEQUENCING PLATFORMS ==&lt;br /&gt;
&lt;br /&gt;
&amp;lt;!-- ═══════════════════════════════ ILLUMINA ═══════════════════════════════ --&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Illumina ==&lt;br /&gt;
&amp;lt;span id=&amp;quot;ILLUMINA&amp;quot;&amp;gt;&amp;lt;/span&amp;gt;&lt;br /&gt;
&lt;br /&gt;
=== NovaSeqX Plus ===&lt;br /&gt;
&lt;br /&gt;
NovaSeqX Plus sequencing is available through BMC&#039;s collaboration with local academic core facilities, and is well suited to projects requiring the deepest coverage or largest sample counts.&lt;br /&gt;
&lt;br /&gt;
* Contact [mailto:biomicro@mit.edu biomicro@mit.edu] to request access and discuss flowcell sharing.&lt;br /&gt;
* See [[MIT:Pricing]] or [[BioMicroCenter:Pricing]] for current lane and flowcell pricing.&lt;br /&gt;
* Full flowcells (8 lanes, 10B or 25B) have priority over individual lanes; wait times for single lanes can vary.&lt;br /&gt;
* Minimum 2 nM concentration; 110 µL per lane. BMC QC required for read guarantees.&lt;br /&gt;
&lt;br /&gt;
=== MiSeq i100 ===&lt;br /&gt;
&lt;br /&gt;
{|&lt;br /&gt;
 |- style=&amp;quot;vertical-align:top;&amp;quot;&lt;br /&gt;
 | style=&amp;quot;width:370px;&amp;quot; |&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
 ! Service&lt;br /&gt;
 ! MiSeq i100 Sequencing&lt;br /&gt;
 |-&lt;br /&gt;
 | INPUT || Illumina libraries&lt;br /&gt;
 |-&lt;br /&gt;
 | MIN VOLUME || 40 µL&lt;br /&gt;
 |-&lt;br /&gt;
 | MIN CONCENTRATION || 2 nM (~0.4 ng/µL for 300 bp library)&lt;br /&gt;
 |-&lt;br /&gt;
 | INCLUDED SERVICES&lt;br /&gt;
 |&lt;br /&gt;
* Quality Control: Fragment Analyzer and qPCR&lt;br /&gt;
* Illumina Sequencing&lt;br /&gt;
* Demultiplexing&lt;br /&gt;
 |-&lt;br /&gt;
 | DATA FORMATS&lt;br /&gt;
 |&lt;br /&gt;
* FASTQ&lt;br /&gt;
* BCL (stored 30 days)&lt;br /&gt;
 |-&lt;br /&gt;
 | PRICING || [[MIT:Pricing]] · [[BioMicroCenter:Pricing]]&lt;br /&gt;
 |-&lt;br /&gt;
 | SUBMISSION&lt;br /&gt;
 |&lt;br /&gt;
* MIT (Assisted) – [https://mit.ilabsolutions.com/service_item/new/3381?spt_id=3863 iLabs]&lt;br /&gt;
* MIT (Walkup) – [https://mit.ilabsolutions.com/schedules/555535#/schedule/ iLabs calendar] (training required)&lt;br /&gt;
 |-&lt;br /&gt;
|}&lt;br /&gt;
 |&lt;br /&gt;
&lt;br /&gt;
The MiSeq i100 uses Illumina&#039;s XLEAP-SBS chemistry on a patterned (CMOS nanowell) flowcell. It is the BMC&#039;s walkup-capable instrument, suited to amplicon panels, 16S rRNA surveys, validation runs, and applications requiring long reads (up to 1000 nt).&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Kits available:&#039;&#039;&#039; 100 nt (25M, 100M), 300 nt (5M, 25M, 100M), 600 nt (5M, 25M, 50M), 1000 nt (25M).&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Walkup service&#039;&#039;&#039; is not available to external users. Runs must be scheduled in the [https://mit.ilabsolutions.com/schedules/555535#/schedule/ iLabs calendar].&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Thanks to:&#039;&#039;&#039; MIT VPR, Drs. Gene Li, Michael Birnbaum, Dept. of Biology, Scott Ritterbush &#039;89 SM &#039;92&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
=== MiSeq classic ===&lt;br /&gt;
&lt;br /&gt;
The MiSeq classic uses 4-color SBS chemistry on a non-patterned (random cluster) flowcell. It is available for users with established protocols on v2/v3 chemistry, but is off contract and will be retired upon instrument failure.&lt;br /&gt;
&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
 ! Kit&lt;br /&gt;
 ! Approx. reads&lt;br /&gt;
 ! Notes&lt;br /&gt;
 |-&lt;br /&gt;
 | 70 nt (V2) || ~5M || 30 or 70 SE&lt;br /&gt;
 |-&lt;br /&gt;
 | 150 nt (V3) || ~5M || 75 PE&lt;br /&gt;
 |-&lt;br /&gt;
 | 300 nt (V2) || ~5M || 150 PE&lt;br /&gt;
 |-&lt;br /&gt;
 | 500 nt (V2) || ~10M || 250 PE&lt;br /&gt;
 |-&lt;br /&gt;
 | 600 nt (V3) || ~15M || 300 PE&lt;br /&gt;
 |-&lt;br /&gt;
 | 500 nt NANO || ~1M || Reduced output run&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
See [[MIT:Pricing]] · [[BioMicroCenter:Pricing]] for kit pricing.&lt;br /&gt;
&lt;br /&gt;
&amp;lt;!-- Donor information for MiSeq classic is not recorded in current source pages. Please update if known. --&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;!-- ═══════════════════════════ ELEMENT BIOSCIENCES ═══════════════════════ --&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Element Biosciences ==&lt;br /&gt;
&amp;lt;span id=&amp;quot;ELEMENT&amp;quot;&amp;gt;&amp;lt;/span&amp;gt;&lt;br /&gt;
&lt;br /&gt;
=== AVITI24 ===&lt;br /&gt;
&lt;br /&gt;
{|&lt;br /&gt;
 |- style=&amp;quot;vertical-align:top;&amp;quot;&lt;br /&gt;
 | style=&amp;quot;width:370px;&amp;quot; |&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
 ! Service&lt;br /&gt;
 ! Element Sequencing&lt;br /&gt;
 |-&lt;br /&gt;
 | SUBMISSION || Illumina (P5/P7) libraries&lt;br /&gt;
 |-&lt;br /&gt;
 | MIN VOLUME || 15 µL&lt;br /&gt;
 |-&lt;br /&gt;
 | MIN CONCENTRATION || 2 nM&lt;br /&gt;
 |-&lt;br /&gt;
 | INCLUDED SERVICES&lt;br /&gt;
 |&lt;br /&gt;
* Quality Control: Fragment Analyzer and qPCR&lt;br /&gt;
* Element Sequencing&lt;br /&gt;
* Demultiplexing&lt;br /&gt;
 |-&lt;br /&gt;
 | ADDITIONAL SERVICES ||&lt;br /&gt;
* Quality Control&lt;br /&gt;
* Element library preparation&lt;br /&gt;
 |-&lt;br /&gt;
 | DATA FORMATS&lt;br /&gt;
 |&lt;br /&gt;
* FASTQ (stored 1 year)&lt;br /&gt;
 |-&lt;br /&gt;
 | QUALITY CONTROL&lt;br /&gt;
 |&lt;br /&gt;
* [https://www.bioinformatics.babraham.ac.uk/projects/fastqc/ FASTQC]&lt;br /&gt;
* Basic run metrics (alignment rate, complexity)&lt;br /&gt;
* Basic RNAseq metrics (where applicable)&lt;br /&gt;
* Basic paired end metrics (where applicable)&lt;br /&gt;
* Contamination checks&lt;br /&gt;
 |-&lt;br /&gt;
 | PRICING || [[MIT:Pricing]] · [[BioMicroCenter:Pricing]]&lt;br /&gt;
 |-&lt;br /&gt;
 | SUBMISSION&lt;br /&gt;
 |&lt;br /&gt;
* MIT – [https://mit.ilabsolutions.com/service_item/new/3381?spt_id=3863 iLabs]&lt;br /&gt;
 |-&lt;br /&gt;
|}&lt;br /&gt;
 |&lt;br /&gt;
=== ANCHORS ===&lt;br /&gt;
Element supports both Illumina anchors (P5/P7) using Freestyle chemistry and Element anchors (SP5/SP27) for Adept chemistry. For standard runs at the BMC, P5/P7-anchored (Illumina) libraries are preferred. Element Adept libraries (SP5/SP27) require a 5′-phosphate on the forward end to circularize.&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
&lt;br /&gt;
=== INDEXING ===&lt;br /&gt;
Compared to Illumina, AVITI24 read direction is similar to Illumina&#039;s Forward Strand workflow for Dual Indexed Libraries. Indexes are read from antisense primers — P7 side first (index 1). Index sequences should be provided &#039;&#039;&#039;as read&#039;&#039;&#039;, not as ordered.&amp;lt;BR&amp;gt;&lt;br /&gt;
* Custom primer submission: 70 µL at 100 µM for Read 1; all other reads require 40 µL at 100 µM.&lt;br /&gt;
&lt;br /&gt;
=== SINGLE LANE ===&lt;br /&gt;
Each lane of the AVITI24 can be loaded independently; both lanes must be filled to run a flowcell. 300PE kits support only a single lane. Custom primers must not interfere with other users&#039; samples. All non-standard requests require a full flowcell.&lt;br /&gt;
&lt;br /&gt;
=== MINIMUM READ GUARANTEE ===&lt;br /&gt;
Guaranteed if: (a) BMC performed QC, (b) library diversity is high (especially in the first 5 bases of Read 1), and (c) concentration ≥ 2 nM.&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Thanks to:&#039;&#039;&#039; Element Biosciences; Scott Ritterbush&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
&amp;lt;!-- ═══════════════════════════ SINGULAR GENOMICS ════════════════════════ --&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Singular Genomics ==&lt;br /&gt;
&amp;lt;span id=&amp;quot;SINGULAR&amp;quot;&amp;gt;&amp;lt;/span&amp;gt;&lt;br /&gt;
&lt;br /&gt;
=== G4 ===&lt;br /&gt;
&lt;br /&gt;
{|&lt;br /&gt;
 |- style=&amp;quot;vertical-align:top;&amp;quot;&lt;br /&gt;
 | style=&amp;quot;width:370px;&amp;quot; |&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
 ! Service&lt;br /&gt;
 ! Singular G4 Sequencing&lt;br /&gt;
 |-&lt;br /&gt;
 | INPUT || Illumina (P5/P7) libraries&lt;br /&gt;
 |-&lt;br /&gt;
 | MIN VOLUME || 12 µL&lt;br /&gt;
 |-&lt;br /&gt;
 | MIN CONCENTRATION || 4 nM&lt;br /&gt;
 |-&lt;br /&gt;
 | INCLUDED SERVICES&lt;br /&gt;
 |&lt;br /&gt;
* Quality Control: Fragment Analyzer and qPCR&lt;br /&gt;
* Pool conversion: P5/P7 → S1/S2 by PCR&lt;br /&gt;
* Singular Sequencing&lt;br /&gt;
* Demultiplexing&lt;br /&gt;
 |-&lt;br /&gt;
 | ADDITIONAL SERVICES ||&lt;br /&gt;
* Quality Control&lt;br /&gt;
* Singular library preparation&lt;br /&gt;
 |-&lt;br /&gt;
 | DATA FORMATS&lt;br /&gt;
 |&lt;br /&gt;
* FASTQ (stored 1 year)&lt;br /&gt;
 |-&lt;br /&gt;
 | QUALITY CONTROL&lt;br /&gt;
 |&lt;br /&gt;
* [https://www.bioinformatics.babraham.ac.uk/projects/fastqc/ FASTQC]&lt;br /&gt;
* Basic run metrics (alignment rate, complexity)&lt;br /&gt;
* Basic RNAseq metrics (where applicable)&lt;br /&gt;
* Contamination checks&lt;br /&gt;
 |-&lt;br /&gt;
 | PRICING || [[MIT:Pricing]] · [[BioMicroCenter:Pricing]]&lt;br /&gt;
 |-&lt;br /&gt;
 | SUBMISSION&lt;br /&gt;
 |&lt;br /&gt;
* MIT – [https://mit.ilabsolutions.com/service_item/new/3381?spt_id=3863 iLabs]&lt;br /&gt;
 |-&lt;br /&gt;
|}&lt;br /&gt;
 |&lt;br /&gt;
=== LIBRARY SUBMISSION ===&lt;br /&gt;
Submit standard Illumina (P5/P7) libraries. The BMC will convert the pool to S1/S2 anchors by PCR prior to sequencing — do &#039;&#039;&#039;not&#039;&#039;&#039; submit S1/S2-anchored libraries directly.&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
&lt;br /&gt;
=== INDEXING ===&lt;br /&gt;
Indexes are read from the S1/S2 anchor sequences after conversion. Index 1 is read from S1, index 2 from S2. Index sequences are the &#039;&#039;&#039;same orientation&#039;&#039;&#039; as ordered in classical Illumina library preps. Dual indexes (8 nt) are required for all lane-by-lane sequencing. Custom index primers are not permitted.&lt;br /&gt;
&lt;br /&gt;
=== LANE BY LANE ===&lt;br /&gt;
* Must be 50 PE or 150 PE dual-indexed.&lt;br /&gt;
* Pool must be compatible with TruSeq, Nextera, smRNA, or Solexa sequencing primers.&lt;br /&gt;
* 4 lanes per flowcell; up to 4 flowcells per run.&lt;br /&gt;
&lt;br /&gt;
=== MINIMUM READ GUARANTEE ===&lt;br /&gt;
Guaranteed if: (a) BMC performed QC, (b) no custom primers, and (c) concentration ≥ 4 nM.&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Thanks to:&#039;&#039;&#039; Singular Genomics&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
== PLATFORM COMPARISON ==&lt;br /&gt;
&amp;lt;span id=&amp;quot;PLATFORM_COMPARISON&amp;quot;&amp;gt;&amp;lt;/span&amp;gt;&lt;br /&gt;
&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; style=&amp;quot;width:100%; text-align:center;&amp;quot;&lt;br /&gt;
|-&lt;br /&gt;
! style=&amp;quot;background:#dce8f5;&amp;quot; | SPEC&lt;br /&gt;
! style=&amp;quot;background:#e8f0fe;&amp;quot; | NovaSeqX Plus&lt;br /&gt;
! style=&amp;quot;background:#e6f4ea;&amp;quot; | Element AVITI24&lt;br /&gt;
! style=&amp;quot;background:#fff3e0;&amp;quot; | Singular G4&lt;br /&gt;
! style=&amp;quot;background:#fff3e0;&amp;quot; | MiSeq i100&lt;br /&gt;
! style=&amp;quot;background:#fff3e0;&amp;quot; | MiSeq classic&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;Read lengths&#039;&#039;&#039;&lt;br /&gt;
| 100 nt, 300 nt&lt;br /&gt;
| 75 PE, 150 PE, 300 PE&lt;br /&gt;
| 50 PE, 150 PE&lt;br /&gt;
| 100–1000 nt&lt;br /&gt;
| 70–600 nt&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;Flowcell type&#039;&#039;&#039;&lt;br /&gt;
| Patterned (X-Amp)&lt;br /&gt;
| Random&lt;br /&gt;
| Patterned&lt;br /&gt;
| Patterned (CMOS nanowell)&lt;br /&gt;
| Non-patterned (random cluster)&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;Chemistry&#039;&#039;&#039;&lt;br /&gt;
| 2-color XLEAP-SBS (G=dark)&lt;br /&gt;
| 4-color avidity SBS&lt;br /&gt;
| 4-color SBS&lt;br /&gt;
| 2-color XLEAP-SBS (G=dark)&lt;br /&gt;
| 4-color SBS&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;Index hopping&#039;&#039;&#039;&lt;br /&gt;
| High — UDIs required&lt;br /&gt;
| Low — UDIs not needed&lt;br /&gt;
| Low (patterned, non-XLEAP)&lt;br /&gt;
| Present — UDIs recommended&lt;br /&gt;
| Low (non-patterned)&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;Low-complexity libraries&#039;&#039;&#039;&lt;br /&gt;
| Struggles&lt;br /&gt;
| Handles well&lt;br /&gt;
| Handles well&lt;br /&gt;
| Struggles&lt;br /&gt;
| Handles well&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;Lane flexibility&#039;&#039;&#039;&lt;br /&gt;
| Lane-by-lane (full FC priority)&lt;br /&gt;
| Lane-by-lane&lt;br /&gt;
| Lane-by-lane&lt;br /&gt;
| Full flowcell only&lt;br /&gt;
| Full flowcell only&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;Walkup&#039;&#039;&#039;&lt;br /&gt;
| No (collaboration)&lt;br /&gt;
| No&lt;br /&gt;
| No&lt;br /&gt;
| Yes (training required)&lt;br /&gt;
| No&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;Illumina lib compatible&#039;&#039;&#039;&lt;br /&gt;
| Yes (native)&lt;br /&gt;
| Yes (P5/P7 Freestyle)&lt;br /&gt;
| Yes — BMC converts pool by PCR&lt;br /&gt;
| Yes (native)&lt;br /&gt;
| Yes (native)&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;Donated / supported by&#039;&#039;&#039;&lt;br /&gt;
| Local core collaboration&lt;br /&gt;
| Element Biosciences; Scott Ritterbush&lt;br /&gt;
| Singular Genomics&lt;br /&gt;
| MIT VPR; Drs. Gene Li, Michael Birnbaum; Dept. of Biology; Scott Ritterbush &#039;89 SM &#039;92&lt;br /&gt;
| &amp;lt;!-- unknown: please update --&amp;gt;&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
== FAQ ==&lt;br /&gt;
&amp;lt;span id=&amp;quot;FAQ&amp;quot;&amp;gt;&amp;lt;/span&amp;gt;&lt;br /&gt;
&lt;br /&gt;
=== How do I choose the right platform? ===&lt;br /&gt;
Start with your &#039;&#039;&#039;read count requirement&#039;&#039;&#039; and &#039;&#039;&#039;library type&#039;&#039;&#039;:&lt;br /&gt;
* &#039;&#039;&#039;Millions of reads or fewer&#039;&#039;&#039; → MiSeq i100 (amplicons, long reads up to 1000 nt) or Singular G4 (most cost-effective for standard 50/150 PE).&lt;br /&gt;
* &#039;&#039;&#039;Hundreds of millions of reads&#039;&#039;&#039; → Element AVITI24 (lane-by-lane flexibility, low index hopping, tolerates low-complexity libraries).&lt;br /&gt;
* &#039;&#039;&#039;Billions of reads&#039;&#039;&#039; → NovaSeqX Plus (contact BMC to coordinate flowcell sharing).&lt;br /&gt;
&lt;br /&gt;
=== Can I use my existing Illumina libraries? ===&lt;br /&gt;
* &#039;&#039;&#039;AVITI24&#039;&#039;&#039; – Yes. P5/P7 libraries run natively using Freestyle chemistry.&lt;br /&gt;
* &#039;&#039;&#039;MiSeq i100 / classic&#039;&#039;&#039; – Yes. Standard Illumina libraries run natively.&lt;br /&gt;
* &#039;&#039;&#039;Singular G4&#039;&#039;&#039; – Yes. Submit standard P5/P7 libraries; the BMC converts the pool to S1/S2 anchors by PCR. Do not submit S1/S2 libraries directly.&lt;br /&gt;
* &#039;&#039;&#039;NovaSeqX Plus&#039;&#039;&#039; – Yes. Standard Illumina libraries run natively.&lt;br /&gt;
&lt;br /&gt;
=== What are the minimum submission requirements? ===&lt;br /&gt;
* AVITI24: 15 µL at 2 nM&lt;br /&gt;
* MiSeq i100 / classic: 40 µL at 2 nM&lt;br /&gt;
* Singular G4: 12 µL at 4 nM&lt;br /&gt;
* NovaSeqX Plus: 110 µL per lane at 2 nM (contact BMC)&lt;br /&gt;
&lt;br /&gt;
=== My library has low complexity. Which platform should I use? ===&lt;br /&gt;
Avoid the NovaSeqX Plus and MiSeq i100 — both use 2-color XLEAP-SBS chemistry where G bases are inferred from signal absence, making low-diversity early cycles prone to run failure. The AVITI24, Singular G4, and MiSeq classic all use 4-color chemistry and handle low-complexity libraries well. Always contact [mailto:biomicro@mit.edu biomicro@mit.edu] before submitting unconventional libraries.&lt;br /&gt;
&lt;br /&gt;
=== Do I need unique dual indexes (UDIs)? ===&lt;br /&gt;
* &#039;&#039;&#039;NovaSeqX Plus&#039;&#039;&#039; – Yes, required (patterned X-Amp flowcell).&lt;br /&gt;
* &#039;&#039;&#039;MiSeq i100&#039;&#039;&#039; – Recommended (patterned CMOS flowcell).&lt;br /&gt;
* &#039;&#039;&#039;AVITI24&#039;&#039;&#039; – Not required; index hopping is low on random flowcells.&lt;br /&gt;
* &#039;&#039;&#039;Singular G4&#039;&#039;&#039; – Not required; patterned but does not use X-Amp chemistry; hopping is on par with HiSeq/GAIIx.&lt;br /&gt;
* &#039;&#039;&#039;MiSeq classic&#039;&#039;&#039; – Not required; non-patterned flowcell has low hopping.&lt;br /&gt;
&lt;br /&gt;
=== How long until my data is delivered? ===&lt;br /&gt;
Queue times are typically short — most samples run within a few days. Full flowcells have sequencing priority over individual lanes. Contact [mailto:biomicro@mit.edu biomicro@mit.edu] for current wait times.&lt;br /&gt;
&lt;br /&gt;
=== How long is my data stored? ===&lt;br /&gt;
* FASTQ files: 1 year for AVITI24, Singular G4, MiSeq i100.&lt;br /&gt;
* BCL files (MiSeq): 30 days.&lt;br /&gt;
* Long-term storage is available; see [[BioMicroCenter:Pricing]] for active storage and tape archive options.&lt;br /&gt;
&lt;br /&gt;
=== What quality control is included? ===&lt;br /&gt;
All runs include a final pool QC (RT-PCR and Fragment Analyzer) and post-sequencing QC (FastQC, run metrics, alignment rate, complexity, RNAseq metrics where applicable, contamination checks). Users who opt out of pre-sequencing QC by providing their own concentration forfeit read guarantees.&lt;br /&gt;
&lt;br /&gt;
=== Can I pool samples from multiple labs? ===&lt;br /&gt;
Yes, but users are responsible for minimizing index cross-contamination and [https://www.illumina.com/science/education/minimizing-index-hopping.html index crosstalk]. The BMC does not combine projects within a lane.&lt;br /&gt;
&lt;br /&gt;
== SUBMISSION ==&lt;br /&gt;
&lt;br /&gt;
All sequencing is submitted through [https://mit.ilabsolutions.com/service_item/new/3381?spt_id=3863 iLabs]. External users should contact [mailto:biomicro@mit.edu biomicro@mit.edu] directly. Samples must meet the minimum volume and concentration requirements listed above; coordinate with BMC staff before sending non-standard submissions.&lt;br /&gt;
&lt;br /&gt;
Additional services:&lt;br /&gt;
* [[BioMicroCenter:Illumina_Library_Preparation|Short read library preparation]]&lt;br /&gt;
* [[BioMicroCenter:BioInformaticsStaff|Bioinformatics support]]&lt;br /&gt;
* [[BioMicroCenter:Servers|Data storage and computation]]&lt;br /&gt;
* [[BioMicroCenter:Sequencing_Quality_Control|Sequencing quality control]]&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:SpTx&amp;diff=131369</id>
		<title>BioMicroCenter:SpTx</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:SpTx&amp;diff=131369"/>
		<updated>2026-04-17T18:21:23Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: Add Singular G4X in situ spatial sequencing section (beta stub)&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;{{BioMicroCenter}}&lt;br /&gt;
 &lt;br /&gt;
The BioMicro Center supports two complementary approaches to spatial transcriptomics: [[BioMicroCenter:SpTx#10X Visium HD|10x Visium HD]] from 10x Genomics for slide-based spatial gene expression, and [[BioMicroCenter:SpTx#AVITI24 In Situ Sequencing|AVITI24 in situ sequencing]] from Element Biosciences. The choice of method depends on your tissue type, resolution requirements, and experimental goals. We strongly encourage consultation with BMC staff prior to beginning a spatial experiment.&lt;br /&gt;
 &lt;br /&gt;
== 10X VISIUM HD ==&lt;br /&gt;
{| width=&amp;quot;100%&amp;quot;&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;width: 200px;&amp;quot;|&lt;br /&gt;
[[File:VisiumHD_workflow_slide.jpg|left|250px|Workflow and capture areas for Visium HD]]&lt;br /&gt;
&amp;lt;div style=&amp;quot;clear:both; padding-top:4px; font-size:12px; color:#555;&amp;quot;&amp;gt;Workflow and capture areas for Visium HD&amp;lt;/div&amp;gt;&lt;br /&gt;
|&lt;br /&gt;
[https://www.10xgenomics.com/products/visium-hd-spatial-gene-expression 10x Visium HD] is a spatial transcriptomics platform that profiles whole-transcriptome gene expression across intact tissue sections at single-cell-scale resolution. Tissue sections are processed through the CytAssist instrument, which transfers RNA complexes onto a capture slide containing a continuous array of 3,000,000 spatially barcoded 2 µm squares covering a 6.5 × 6.5 mm capture area (~0.65 cm²). Two chemistries are available: a probe-based assay for human and mouse (FF, FxF, and FFPE), and a polyA-based assay (Visium HD 3&#039;) for fresh frozen tissue from any species. H&amp;amp;E or immunofluorescence (IF) staining can be performed prior to processing for protein co-detection.&lt;br /&gt;
&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
|}&lt;br /&gt;
 &lt;br /&gt;
{|&lt;br /&gt;
 |- style=&amp;quot;vertical-align: top;&amp;quot;&lt;br /&gt;
 |style=&amp;quot;width: 500px;&amp;quot;|&lt;br /&gt;
 &lt;br /&gt;
=== ARRAY SPECIFICATIONS ===&lt;br /&gt;
 {| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
  ! Capture area&lt;br /&gt;
  | 6.5 × 6.5 mm (~0.65 cm²) per tissue&lt;br /&gt;
  |-&lt;br /&gt;
  ! Spots&lt;br /&gt;
  | 3,000,000 barcoded squares&lt;br /&gt;
  |-&lt;br /&gt;
  ! Spot size&lt;br /&gt;
  | 2 µm continuous (no gaps)&lt;br /&gt;
  |-&lt;br /&gt;
  ! Default analysis bins&lt;br /&gt;
  | 8 µm and 16 µm (SpaceRanger); custom bins supported&lt;br /&gt;
  |-&lt;br /&gt;
  ! Tissues per slide&lt;br /&gt;
  | 2&lt;br /&gt;
  |-&lt;br /&gt;
  ! Multiomics&lt;br /&gt;
  | H&amp;amp;E or IF staining prior to processing; antibody panels (FFPE, human only)&lt;br /&gt;
 |}&lt;br /&gt;
 &lt;br /&gt;
=== CHEMISTRY OPTIONS ===&lt;br /&gt;
 {| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
  !&lt;br /&gt;
  ! Visium HD (probe-based)&lt;br /&gt;
  ! Visium HD 3&#039; (polyA-based)&lt;br /&gt;
  |-&lt;br /&gt;
  ! Species&lt;br /&gt;
  | Human, Mouse only&lt;br /&gt;
  | Any species&lt;br /&gt;
  |-&lt;br /&gt;
  ! Tissue formats&lt;br /&gt;
  | Fresh Frozen (FF), Fixed Frozen (FxF), FFPE&lt;br /&gt;
  | Fresh Frozen (FF) only&lt;br /&gt;
  |-&lt;br /&gt;
  ! Notes&lt;br /&gt;
  | ~3 probes per gene; exogenous sequences (GFP, reporters) require custom probes. A small fraction of genes are excluded by default due to predicted off-target activity: [https://www.10xgenomics.com/support/spatial-gene-expression-hd/documentation/steps/probe-sets/visium-human-transcriptome-probe-set-v2-1 human excluded gene list] (~2.1%), [https://www.10xgenomics.com/support/spatial-gene-expression-hd/documentation/steps/probe-sets/visium-mouse-transcriptome-probe-set-v2-0 mouse excluded gene list] (~1.8%).&lt;br /&gt;
  | Species-agnostic; captures polyadenylated transcripts&lt;br /&gt;
 |}&lt;br /&gt;
 &lt;br /&gt;
=== TISSUE PREPARATION REQUIREMENTS ===&lt;br /&gt;
 {| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
  !&lt;br /&gt;
  ! Fresh Frozen (FF)&lt;br /&gt;
  ! Fixed Frozen (FxF)&lt;br /&gt;
  ! FFPE&lt;br /&gt;
  |-&lt;br /&gt;
  ! Section thickness&lt;br /&gt;
  | 10–20 µm &amp;lt;BR&amp;gt;&amp;lt;small&amp;gt;10 µm recommended&amp;lt;/small&amp;gt;&lt;br /&gt;
  | 10–20 µm&lt;br /&gt;
  | 5 µm&lt;br /&gt;
  |-&lt;br /&gt;
  ! Storage&lt;br /&gt;
  | OCT, −80°C&lt;br /&gt;
  | −80°C&lt;br /&gt;
  | Room temp&lt;br /&gt;
  |-&lt;br /&gt;
  ! RNA quality&lt;br /&gt;
  | colspan=&amp;quot;3&amp;quot; | DV200 ≥ 30% recommended; higher scores improve sensitivity&lt;br /&gt;
  |-&lt;br /&gt;
  ! Histology&lt;br /&gt;
  | colspan=&amp;quot;3&amp;quot; | Sectioning &amp;amp; H&amp;amp;E performed by the [https://ki-sbc.mit.edu/histology Tang Histology Facility] (KI). Coordinate with BMC staff to arrange.&lt;br /&gt;
 |}&lt;br /&gt;
 &lt;br /&gt;
For current pricing, see [[BioMicroCenter:Pricing]] → Single Cell &amp;amp; Spatial Services → Visium. Sequencing is charged separately; 400–800M reads per tile recommended for Visium HD.&lt;br /&gt;
 &lt;br /&gt;
=== SUBMISSION ===&lt;br /&gt;
Submit via [https://mit.ilabsolutions.com/service_item/new/3381?spt_id=12903 iLabs (assisted service)]. Coordinate with BMC staff at least one week in advance. Tissue processing begins at the [https://ki-sbc.mit.edu/histology Tang Histology Facility]; please arrange sectioning separately.&lt;br /&gt;
 &lt;br /&gt;
|&lt;br /&gt;
 &lt;br /&gt;
=== 10X Visium HD ===&lt;br /&gt;
[[File:VisiumHD_loupe_example.jpg|thumb|right|350px|Example of FF data from Visium HD]]&lt;br /&gt;
 &lt;br /&gt;
&#039;&#039;&#039;FAQs for Users&#039;&#039;&#039;&amp;lt;BR&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;1) What tissues are compatible?&#039;&#039;&#039;&amp;lt;br&amp;gt;&lt;br /&gt;
Visium HD (probe-based) supports human and mouse tissues in FF, FxF, and FFPE formats. Visium HD 3&#039; (polyA) supports fresh frozen tissue from any species. 10x Genomics maintains a [https://www.10xgenomics.com/support/spatial-gene-expression-hd/documentation/steps/tissue-prep-for-fresh-frozen/visium-hd-spatial-gene-expression-tested-tissues-fresh-frozen list of tested tissues]. Additional optimization may be required for tissues with high fat content. Consult BMC staff before starting.&lt;br /&gt;
&amp;lt;br&amp;gt;&amp;lt;br&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;2) How do I prepare and submit tissue?&#039;&#039;&#039;&amp;lt;br&amp;gt;&lt;br /&gt;
Tissue sectioning and H&amp;amp;E staining are performed by the [https://ki-sbc.mit.edu/histology Hope Babette Tang (1983) Histology Facility] at the Koch Institute. Coordinate with both the Histology Facility and BMC staff before your first submission. FF tissue should be embedded in OCT and stored at −80°C; FFPE blocks at room temperature.&lt;br /&gt;
&amp;lt;br&amp;gt;&amp;lt;br&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;3) What is the resolution?&#039;&#039;&#039;&amp;lt;br&amp;gt;&lt;br /&gt;
Visium HD captures data at 2 µm bin resolution (single-cell scale). SpaceRanger aggregates into 8 µm and 16 µm bins for higher sensitivity by default; custom bin sizes are also supported. Cell segmentation based on H&amp;amp;E imaging is available in the Loupe Browser pipeline.&lt;br /&gt;
&amp;lt;br&amp;gt;&amp;lt;br&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;4) How much sequencing is needed?&#039;&#039;&#039;&amp;lt;br&amp;gt;&lt;br /&gt;
400–800M reads per tile is recommended for Visium HD. Sequencing is submitted separately via iLabs. Compatible platforms include NovaSeq SP, NovaSeqX, and Element AVITI. Discuss read depth with BMC staff based on your tissue type and goals.&lt;br /&gt;
&amp;lt;br&amp;gt;&amp;lt;br&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;5) Does Visium HD require permeabilization optimization?&#039;&#039;&#039;&amp;lt;br&amp;gt;&lt;br /&gt;
No. Visium HD does not require permeabilization time optimization. For fresh frozen tissues new to the platform, a test array is available to verify tissue quality and placement before committing a full experiment.&lt;br /&gt;
&amp;lt;br&amp;gt;&amp;lt;br&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;6) Can I detect proteins alongside gene expression?&#039;&#039;&#039;&amp;lt;br&amp;gt;&lt;br /&gt;
Yes. H&amp;amp;E or IF staining can be performed prior to CytAssist processing. Antibody panels for protein co-detection (human tissues, FFPE only) are available from 10x Genomics. Discuss your experimental design with BMC staff in advance.&lt;br /&gt;
&amp;lt;br&amp;gt;&amp;lt;br&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;7) What analysis outputs are provided?&#039;&#039;&#039;&amp;lt;br&amp;gt;&lt;br /&gt;
The BMC delivers FASTQ files, aligned BAM files, the spatial feature-barcode matrix (raw and filtered at 2, 8, and 16 µm bins), and a Loupe Browser file. Downstream informatics support is available through BMC staff.&lt;br /&gt;
&amp;lt;br&amp;gt;&amp;lt;br&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;8) Can I combine Visium HD with single-cell data?&#039;&#039;&#039;&amp;lt;br&amp;gt;&lt;br /&gt;
Yes. Visium HD data integrates with single-cell or single-nucleus RNA-seq (e.g., 10x Chromium Flex) for cell-type deconvolution. Starting from the same tissue block, nuclei can be isolated for Flex single-cell gene expression. Coordinate with BMC staff if planning a multi-modal experiment.&lt;br /&gt;
&amp;lt;br&amp;gt;&amp;lt;br&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;9) My gene of interest is exogenous (GFP, reporter, viral) or missing from the probe set. What are my options?&#039;&#039;&#039;&amp;lt;br&amp;gt;&lt;br /&gt;
The probe-based Visium HD assay does not capture exogenous sequences by default. Custom probes can be designed for detection — see 10x Genomics technical note CG000621. Additionally, a small fraction of endogenous genes are excluded from the standard probe sets due to predicted off-target activity: see the [https://www.10xgenomics.com/support/spatial-gene-expression-hd/documentation/steps/probe-sets/visium-human-transcriptome-probe-set-v2-1 human excluded gene list] (~2.1% of genes) and [https://www.10xgenomics.com/support/spatial-gene-expression-hd/documentation/steps/probe-sets/visium-mouse-transcriptome-probe-set-v2-0 mouse excluded gene list] (~1.8% of genes). Alternatively, the polyA-based Visium HD 3&#039; assay captures all polyadenylated transcripts and does not require custom probes.&lt;br /&gt;
&amp;lt;br&amp;gt;&lt;br /&gt;
 &lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
== AVITI24 CYTOPROFILING &amp;amp; IN SITU SEQUENCING ==&lt;br /&gt;
&amp;lt;div style=&amp;quot;background:#fff8e6; border-left:4px solid #c8960c; padding:8px 12px; margin-bottom:12px;&amp;quot;&amp;gt;&#039;&#039;&#039;Beta Service — Early Access:&#039;&#039;&#039; The AVITI24 Teton CytoProfiling and DISS services at the BioMicro Center are operational but not yet routine. &#039;&#039;&#039;Consultation with BMC staff is required before submitting samples.&#039;&#039;&#039;&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;!-- placeholder image left: AVITI24 instrument photo or Teton CytoProfiling schematic --&amp;gt;&lt;br /&gt;
&amp;lt;!-- replace with: [[File:AVITI24_instrument.png|thumb|left|400px]] --&amp;gt;&lt;br /&gt;
&lt;br /&gt;
The BioMicro Center provides access to the [https://www.elementbiosciences.com/products/aviti24 Element Biosciences AVITI24] platform for in situ single-cell multiomics. Using [https://www.elementbiosciences.com/products/aviti24/cytoprofiling Teton™ CytoProfiling], the instrument co-detects RNA transcripts, proteins (including phospho-proteins), and cell morphology at subcellular resolution — directly in intact cells, without library preparation. Targeted in situ sequencing via Direct In Sample Sequencing (DISS) can be run simultaneously in the same experiment. Because this is an early-access service, results and run parameters are less well-established than our established offerings. Consultation with BMC staff is strongly encouraged before designing experiments.&lt;br /&gt;
&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
&lt;br /&gt;
{|&lt;br /&gt;
 |- style=&amp;quot;vertical-align: top;&amp;quot;&lt;br /&gt;
 |style=&amp;quot;width: 500px;&amp;quot;|&lt;br /&gt;
&lt;br /&gt;
=== PLATFORM SPECIFICATIONS ===&lt;br /&gt;
 {| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
  ! Instrument&lt;br /&gt;
  | Element Biosciences AVITI24&lt;br /&gt;
  |-&lt;br /&gt;
  ! Technology&lt;br /&gt;
  | Teton™ CytoProfiling (ABC Sequencing)&lt;br /&gt;
  |-&lt;br /&gt;
  ! Flow cell formats&lt;br /&gt;
  | 1-well · 12-well (96-well format) · 48-well (384-well format)&lt;br /&gt;
  |-&lt;br /&gt;
  ! Imageable area&lt;br /&gt;
  | Up to 10 cm² per flow cell side&lt;br /&gt;
  |-&lt;br /&gt;
  ! Max cells per run&lt;br /&gt;
  | Up to 1 million adherent cells per flow cell&lt;br /&gt;
  |-&lt;br /&gt;
  ! Transcript density&lt;br /&gt;
  | ~100–200 transcripts per 100 µm²&lt;br /&gt;
 |}&lt;br /&gt;
&lt;br /&gt;
=== ASSAY CAPABILITIES ===&lt;br /&gt;
 {| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
  ! style=&amp;quot;width:18%&amp;quot;|&lt;br /&gt;
  ! style=&amp;quot;width:27%; background:#e8f0f8;&amp;quot;| Teton CytoProfiling&lt;br /&gt;
  ! style=&amp;quot;width:27%; background:#e8f4e8;&amp;quot;| DISS (low output) &amp;lt;BR&amp;gt;&amp;lt;small&amp;gt;&#039;&#039;&#039;available&#039;&#039;&#039;&amp;lt;/small&amp;gt;&lt;br /&gt;
  ! style=&amp;quot;width:27%; background:#f5f0f0;&amp;quot;| DISS (high output) &amp;lt;BR&amp;gt;&amp;lt;small&amp;gt;&#039;&#039;not yet released&#039;&#039;&amp;lt;/small&amp;gt;&lt;br /&gt;
  |-&lt;br /&gt;
  ! RNA&lt;br /&gt;
  | 350 transcripts &amp;lt;BR&amp;gt;&amp;lt;small&amp;gt;panel-based&amp;lt;/small&amp;gt;&lt;br /&gt;
  | Targeted genes &amp;lt;BR&amp;gt;&amp;lt;small&amp;gt;~50 nt, 1 priming event; CRISPR guides, barcodes, expressed mutations&amp;lt;/small&amp;gt;&lt;br /&gt;
  | &amp;lt;span style=&amp;quot;color:#999;&amp;quot;&amp;gt;Whole transcriptome &amp;lt;BR&amp;gt;&amp;lt;small&amp;gt;polyT prime; 100–300 nt&amp;lt;/small&amp;gt;&amp;lt;/span&amp;gt;&lt;br /&gt;
  |-&lt;br /&gt;
  ! Protein&lt;br /&gt;
  | colspan=&amp;quot;3&amp;quot; | ~18 targets &amp;amp;nbsp;&amp;lt;small&amp;gt;panels of 24; rabbit monoclonal primary; custom available&amp;lt;/small&amp;gt;&lt;br /&gt;
  |-&lt;br /&gt;
  ! Morphology&lt;br /&gt;
  | colspan=&amp;quot;3&amp;quot; | Nucleus, actin, membrane (all species) · ER, Golgi, mitochondria (human only)&lt;br /&gt;
  |-&lt;br /&gt;
  ! Panels / probes&lt;br /&gt;
  | MAPK Cell Cycle · MAPK Apoptosis · Immuno-Oncology · Neuroscience &amp;lt;BR&amp;gt;&amp;lt;small&amp;gt;mix up to 3 panels per run; consult staff for latest&amp;lt;/small&amp;gt;&lt;br /&gt;
  | User-designed probes&lt;br /&gt;
  | &amp;lt;span style=&amp;quot;color:#999;&amp;quot;&amp;gt;User-designed or polyT probe&amp;lt;/span&amp;gt;&lt;br /&gt;
 |}&lt;br /&gt;
Teton CytoProfiling and DISS (low output) can be run simultaneously in the same experiment. Custom antibody add-ons require unconjugated rabbit monoclonal primaries. Consult BMC staff for current options.&lt;br /&gt;
&lt;br /&gt;
=== SAMPLE REQUIREMENTS ===&lt;br /&gt;
 {| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
  ! Supported&lt;br /&gt;
  | Monolayer cell culture (adherent); pelleted cell suspension (spun down and resuspended)&lt;br /&gt;
  |-&lt;br /&gt;
  ! Not yet supported&lt;br /&gt;
  | Complex 3D culture, organoids, tissue sections — contact BMC to discuss future availability&lt;br /&gt;
  |-&lt;br /&gt;
  ! Cell density&lt;br /&gt;
  | Consult BMC staff; optimized per cell type&lt;br /&gt;
  |-&lt;br /&gt;
  ! Species&lt;br /&gt;
  | Human (all current fixed panels); other species — consult staff regarding panel compatibility&lt;br /&gt;
 |}&lt;br /&gt;
&lt;br /&gt;
=== SUBMISSION ===&lt;br /&gt;
&amp;lt;!-- iLabs link stub: replace once service item is created --&amp;gt;&lt;br /&gt;
&amp;lt;!-- [https://mit.ilabsolutions.com/service_item/new/XXXX iLabs (assisted service)] --&amp;gt;&lt;br /&gt;
Submission via iLabs is not yet available. Contact BMC staff directly to discuss your experiment before scheduling. &#039;&#039;&#039;Consultation is required for all AVITI24 Teton and DISS submissions.&#039;&#039;&#039;&lt;br /&gt;
&lt;br /&gt;
|&lt;br /&gt;
&lt;br /&gt;
=== AVITI24 Teton CytoProfiling at BMC ===&lt;br /&gt;
[[File:AVITI24_Boyer_cardiomyocytes.jpg|thumb|right|300px|Cytoprofiling of cardiomyocytes. Collaboration with the Boyer Lab.]]&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;About the Service&#039;&#039;&#039;&amp;lt;BR&amp;gt;&lt;br /&gt;
The AVITI24 at BMC supports Teton™ CytoProfiling — an in situ multiomics approach that co-detects RNA transcripts, proteins (including phospho-proteins), and cell morphology directly inside intact cells on the flow cell. Each run cycles through Cell Painting (morphology imaging), antibody scoring, and in situ sequencing rounds without any library preparation. A full run takes 24–48 hours end-to-end with approximately 45 minutes of hands-on time, capturing ~100–200 transcripts per 100 µm² at subcellular resolution.&lt;br /&gt;
&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
The platform is designed for cells in monolayer culture or spun-down suspension. It profiles up to 350 RNA targets, ~18 protein targets, and 6 morphology channels simultaneously in a single experiment — giving a co-registered multiomic readout per cell that would otherwise require separate assays on separate instruments.&lt;br /&gt;
&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
Targeted in situ sequencing (DISS, low output) can be layered onto any Teton run. This uses a user-designed probe (~50 nt, 1 priming event) to detect specific sequences in situ — useful for CRISPR guide detection, barcode readout, or expressed mutation calling. Note that reads are too short for TCR/BCR sequencing; consult BMC staff if your application involves immune repertoire.&lt;br /&gt;
&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;Available Assays&#039;&#039;&#039;&amp;lt;BR&amp;gt;&lt;br /&gt;
BMC supports all four currently available fixed Teton panels (MAPK Cell Cycle, MAPK Apoptosis, Immuno-Oncology, Neuroscience). Up to three panels can be combined per run, giving up to 350 RNA targets and ~18 usable protein targets in a single experiment. Custom antibody add-ons are possible using unconjugated rabbit monoclonal primary antibodies — no conjugation or barcoding required. Discuss panel selection with BMC staff; reagent availability and lead time vary.&lt;br /&gt;
&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;Data Outputs&#039;&#039;&#039;&amp;lt;BR&amp;gt;&lt;br /&gt;
The BMC provides core analysis outputs including:&lt;br /&gt;
* Cell-by-gene expression matrix (350 transcript targets per panel)&lt;br /&gt;
* Cell-by-protein parameter data (~18 antibody targets)&lt;br /&gt;
* Morphology feature data per cell (nucleus, actin, membrane; ER, Golgi, mitochondria for human)&lt;br /&gt;
* Spatial maps with morphology channel overlays&lt;br /&gt;
* [https://www.elementbiosciences.com/products/elembio-catalyst ElemBio Catalyst / CytoCanvas]-compatible files for interactive visualization and downstream analysis&lt;br /&gt;
&amp;lt;BR&amp;gt;&lt;br /&gt;
Additional informatics support is available through BMC staff.&lt;br /&gt;
&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;Pricing&#039;&#039;&#039;&amp;lt;BR&amp;gt;&lt;br /&gt;
See [[BioMicroCenter:Pricing|BioMicroCenter:Pricing]] → Single Cell &amp;amp; Spatial Services → AVITI24 for current pricing. Sequencing-only runs on the AVITI24 are charged at standard AVITI24 sequencing rates.&lt;br /&gt;
&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;Service Status&#039;&#039;&#039;&amp;lt;BR&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;background:#fff8e6; border-left:4px solid #c8960c; padding:8px 12px; margin-top:8px;&amp;quot;&amp;gt;This service is in &#039;&#039;&#039;beta&#039;&#039;&#039;. The instrument is operational and BMC has completed initial runs, but experimental parameters are still being validated. We welcome early users willing to work collaboratively with BMC staff to establish protocols.&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
== SINGULAR G4X IN SITU SPATIAL SEQUENCING ==&lt;br /&gt;
&amp;lt;div style=&amp;quot;background:#fff8e6; border-left:4px solid #c8960c; padding:8px 12px; margin-bottom:12px;&amp;quot;&amp;gt;&#039;&#039;&#039;Beta Service — Early Access:&#039;&#039;&#039; The Singular G4X at the BioMicro Center is newly installed and not yet in routine service. &#039;&#039;&#039;Consultation with BMC staff is required before submitting samples.&#039;&#039;&#039;&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;!-- placeholder image left: Singular G4X instrument photo or spatial multiomics schematic --&amp;gt;&lt;br /&gt;
&amp;lt;!-- replace with: [[File:SingularG4X_instrument.png|thumb|left|400px]] --&amp;gt;&lt;br /&gt;
&lt;br /&gt;
The BioMicro Center provides access to the [https://www.singulargenomics.com/g4x Singular Genomics G4X], an upgrade to the G4 sequencer that adds in situ spatial multiomics capability. The G4X simultaneously profiles targeted RNA transcripts, proteins, and fluorescent H&amp;amp;E (fH&amp;amp;E) directly in intact FFPE tissue sections at subcellular resolution. Runs are organized around individual flow cells; BMC will typically run one flow cell at a time. Because this is an early-access service, results and run parameters are less well-established than our routine offerings. Consultation with BMC staff is strongly encouraged before designing experiments.&lt;br /&gt;
&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
&lt;br /&gt;
{|&lt;br /&gt;
 |- style=&amp;quot;vertical-align: top;&amp;quot;&lt;br /&gt;
 |style=&amp;quot;width: 500px;&amp;quot;|&lt;br /&gt;
&lt;br /&gt;
=== FLOW CELL OPTIONS (per slide) ===&lt;br /&gt;
 {| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
  !  Flow cell !! Lanes !! ROI size !! Samples !! Imaging area !! Run time&lt;br /&gt;
  |-&lt;br /&gt;
  ! X2&lt;br /&gt;
  | 2 || 10 × 10 mm || 10 || 10 cm² || ~29 hrs&lt;br /&gt;
  |-&lt;br /&gt;
  ! X4&lt;br /&gt;
  | 4 || 4.5 × 4.5 mm || 32 || 6.5 cm² || ~24 hrs&lt;br /&gt;
 |}&lt;br /&gt;
&amp;lt;small&amp;gt;Run times do not include analysis time (~1 day per flow cell after imaging). Consult BMC staff to select the appropriate flow cell for your project.&amp;lt;/small&amp;gt;&lt;br /&gt;
&lt;br /&gt;
=== PLATFORM SPECIFICATIONS ===&lt;br /&gt;
 {| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
  ! Instrument&lt;br /&gt;
  | [https://www.singulargenomics.com/g4x Singular Genomics G4X]&lt;br /&gt;
  |-&lt;br /&gt;
  ! Spatial modalities&lt;br /&gt;
  | Targeted RNA (in situ), targeted protein, fluorescent H&amp;amp;E (fH&amp;amp;E)&lt;br /&gt;
  |-&lt;br /&gt;
  ! RNA plex&lt;br /&gt;
  | Up to 500-plex per run&lt;br /&gt;
  |-&lt;br /&gt;
  ! Protein plex&lt;br /&gt;
  | Up to 18-plex per run&lt;br /&gt;
  |-&lt;br /&gt;
  ! Tissue format&lt;br /&gt;
  | FFPE (primary); other formats — consult BMC staff&lt;br /&gt;
  |-&lt;br /&gt;
  ! Sample-to-answer&lt;br /&gt;
  | ~5 days&lt;br /&gt;
 |}&lt;br /&gt;
&lt;br /&gt;
For current pricing, see [[BioMicroCenter:Pricing]]. Details to be added as the service matures.&lt;br /&gt;
&lt;br /&gt;
=== SUBMISSION ===&lt;br /&gt;
&amp;lt;!-- iLabs link stub: replace once service item is created --&amp;gt;&lt;br /&gt;
Submission via iLabs is not yet available. Contact BMC staff directly to discuss your experiment before scheduling. &#039;&#039;&#039;Consultation is required for all G4X submissions.&#039;&#039;&#039;&lt;br /&gt;
&lt;br /&gt;
|&lt;br /&gt;
&lt;br /&gt;
=== Singular G4X at BMC ===&lt;br /&gt;
&amp;lt;!-- placeholder image right: G4X example spatial data showing tissue section with RNA/protein overlay --&amp;gt;&lt;br /&gt;
&amp;lt;!-- replace with: [[File:SingularG4X_example.png|thumb|right|350px]] --&amp;gt;&lt;br /&gt;
&lt;br /&gt;
The Singular G4X enables in situ spatial multiomics by co-detecting RNA transcripts, targeted proteins, and fluorescent H&amp;amp;E simultaneously in the same FFPE tissue section at subcellular resolution. Tissue sections are transferred to flow cells using Singular&#039;s TissuStamp&amp;amp;trade; system, and the instrument images the full flow cell in a single day. BMC will typically run one flow cell at a time, with flow cell choice (X2 or X4) determining sample capacity and ROI size.&lt;br /&gt;
&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
The platform uses application-specific RNA panels (290–500 plex depending on tissue type) and a protein panel (up to 18-plex). Panels can be customized with add-ons of up to 50 genes, new 150-gene modules, or fully custom 500-gene panels. Current off-the-shelf panels include Lung, Colon, Breast, Kidney, Pancreas, Skin, and Pan-Mouse. See the [https://www.singulargenomics.com/content Singular content page] for the full panel list and gene targets.&lt;br /&gt;
&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
For more detail on chemistry, workflow, and analysis, see the [https://www.singulargenomics.com/g4x-platform-ecosystem G4X Platform Ecosystem] and [https://www.singulargenomics.com/g4x G4X in situ multiomics] pages from Singular Genomics.&lt;br /&gt;
&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;Service Status&#039;&#039;&#039;&amp;lt;BR&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;background:#fff8e6; border-left:4px solid #c8960c; padding:8px 12px; margin-top:8px;&amp;quot;&amp;gt;This service is in &#039;&#039;&#039;beta&#039;&#039;&#039;. The G4X is newly installed and BMC is establishing protocols and validated run parameters. We welcome early users willing to work collaboratively with BMC staff. Contact biomicro@mit.edu to discuss your project.&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
|}&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=BioMicroCenter&amp;diff=131255</id>
		<title>BioMicroCenter</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=BioMicroCenter&amp;diff=131255"/>
		<updated>2026-04-14T17:23:07Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;&amp;lt;h2 align=&amp;quot;center&amp;quot;&amp;gt; Welcome to the MIT BioMicro Center! &amp;lt;/h2&amp;gt;&lt;br /&gt;
&lt;br /&gt;
{| width=&amp;quot;100%&amp;quot; &lt;br /&gt;
 |- &lt;br /&gt;
 |valign=&amp;quot;top&amp;quot;|&lt;br /&gt;
[[File:logo.png|left]]&lt;br /&gt;
 | valign=&amp;quot;top&amp;quot; align=&amp;quot;left&amp;quot;|&lt;br /&gt;
* [[BioMicroCenter:News|&#039;&#039;&#039;News &amp;amp; General Information&#039;&#039;&#039;]]&lt;br /&gt;
* [[BioMicroCenter:Pricing|Pricing]] / [[MIT:Pricing|MIT Pricing]]&lt;br /&gt;
* [[BioMicroCenter:Submission|Sample Submission]]&lt;br /&gt;
* [[BioMicroCenter:Consumables|Consumables]]&lt;br /&gt;
 | valign=&amp;quot;top&amp;quot; align=&amp;quot;left&amp;quot;|&lt;br /&gt;
* [[BioMicroCenter:AboutBMC|About the BioMicro Center]]&lt;br /&gt;
* [[BioMicroCenter:People| Staff]] &lt;br /&gt;
&amp;lt;!-- [[BioMicroCenter:Jobs|Positions Available]] --&amp;gt;&lt;br /&gt;
* [[BioMicroCenter:FAQ|FAQs]]&lt;br /&gt;
|}&lt;br /&gt;
The &#039;&#039;&#039;MIT BioMicro Center&#039;&#039;&#039; is an integrated genomics core facility that provides both expertise and equipment for systems biology. The core has significant resources in sample preparation, next generation sequencing and in high throughput screening as well as bioinformatics and BioIT. The Center is a joint endeavor between the [http://biology.mit.edu Department of Biology], the [http://ki.mit.edu Koch Institute for Integrative Cancer Research] (Integrated Genomics and Bioinformatics Core (IGB)), and the [http://web.mit.edu/be Department of Biological Engineering].&lt;br /&gt;
&lt;br /&gt;
{| width=&amp;quot;100%&amp;quot;&lt;br /&gt;
 |- valign=&amp;quot;top&amp;quot;&lt;br /&gt;
 |colspan=2 align=&amp;quot;center&amp;quot; style=&amp;quot;background-color:yellow; border:1px solid purple&amp;quot;| &lt;br /&gt;
 {|&lt;br /&gt;
  |&amp;lt;BIG&amp;gt;&amp;lt;B&amp;gt;[[BioMicroCenter:Assisted_Services|Assisted Services]] &amp;lt;/B&amp;gt;&amp;lt;/BIG&amp;gt;&lt;br /&gt;
 |}&lt;br /&gt;
 |align=&amp;quot;center&amp;quot; style=&amp;quot;background-color:aqua; border:1px solid black&amp;quot;|&lt;br /&gt;
 {|&lt;br /&gt;
  |&amp;lt;BIG&amp;gt;&amp;lt;B&amp;gt; Walkup Services &amp;lt;/B&amp;gt;&amp;lt;/BIG&amp;gt;&lt;br /&gt;
 |}&lt;br /&gt;
&lt;br /&gt;
 |-&lt;br /&gt;
 |valign=&amp;quot;top&amp;quot; align=&amp;quot;center&amp;quot; style=&amp;quot;background-color:transparent; border:1px solid black&amp;quot;| &lt;br /&gt;
 {|&lt;br /&gt;
  |width=&amp;quot;10%&amp;quot; align=&amp;quot;left&amp;quot;|&amp;lt;BIG&amp;gt;&amp;lt;B&amp;gt;Sample Isolation&amp;lt;/B&amp;gt;&amp;lt;/BIG&amp;gt;&lt;br /&gt;
* Sonication ([[BioMicroCenter:Covaris|Covaris E220/R230]])&lt;br /&gt;
* DNA/RNA purification ([[BioMicroCenter:Tecan_Freedom_Evo#Revvity_Chemagic_360|Chemagic360]])&lt;br /&gt;
* Quality Control&amp;lt;BR&amp;gt;([[BioMicroCenter:QC|AATI Fragment Analyzer/FemtoPulse]])&lt;br /&gt;
 |}&lt;br /&gt;
 |valign=&amp;quot;top&amp;quot; align=&amp;quot;center&amp;quot; style=&amp;quot;background-color:transparent; border:1px solid black&amp;quot;| &lt;br /&gt;
 {|&lt;br /&gt;
  |width=&amp;quot;10%&amp;quot; align=&amp;quot;left&amp;quot;|&amp;lt;BIG&amp;gt;&amp;lt;B&amp;gt;Library Preparation&amp;lt;/B&amp;gt;&amp;lt;/BIG&amp;gt;&lt;br /&gt;
* [[BioMicroCenter:Illumina_Library_Preparation|Short Read Sample Preparation]]&amp;lt;small&amp;gt;&lt;br /&gt;
** Standard Methods for [[BioMicroCenter:DNA_LIB| *DNA* ]] [[BioMicroCenter:RNA_LIB| *RNA* ]]&lt;br /&gt;
** [[BioMicroCenter:FAQ#STANDARD_versus_HIGH_THROUGHPUT_LIBRARY_PREPARATION|High Throughput Methods]] for [[BioMicroCenter:DNA_HTL| *DNA* ]]  [[BioMicroCenter:RNA_HTL| *RNA* ]]&amp;lt;/small&amp;gt;&lt;br /&gt;
* Long Read Sample Preparation&amp;lt;BR&amp;gt;([[BioMicroCenter:NanoPore_Library_Prep|Nanopore]] and [[BioMicroCenter:PacBio_Library_Preparation|PacBio]])&lt;br /&gt;
 |}&lt;br /&gt;
|valign=&amp;quot;top&amp;quot; align=&amp;quot;center&amp;quot; style=&amp;quot;background-color:transparent; border:1px solid black&amp;quot;| &lt;br /&gt;
 {|&lt;br /&gt;
  |width=&amp;quot;10%&amp;quot; align=&amp;quot;left&amp;quot;|&amp;lt;BIG&amp;gt;&amp;lt;B&amp;gt;[[BioMicroCenter:Walkup Services|Walk-up Instrumentation]]&amp;lt;/B&amp;gt;&amp;lt;/BIG&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;Includes: qPCR (LC480), Plate Readers (Varioskan/Synergy H1)&amp;lt;BR&amp;gt; BioAnalyzer, NanoDrop, Covaris, Pippin Prep&amp;lt;BR&amp;gt; Tecan/Chemagic automation&amp;lt;BR&amp;gt; MiSeq i100, 10x Chromium X&amp;lt;/small&amp;gt;&lt;br /&gt;
 |}&lt;br /&gt;
&lt;br /&gt;
 |-&lt;br /&gt;
 |valign=&amp;quot;top&amp;quot; align=&amp;quot;center&amp;quot; style=&amp;quot;background-color:transparent; border:1px solid black&amp;quot;| &lt;br /&gt;
 {|&lt;br /&gt;
  |width=&amp;quot;10%&amp;quot; align=&amp;quot;left&amp;quot;|&amp;lt;BIG&amp;gt;&amp;lt;B&amp;gt; &lt;br /&gt;
[[BioMicroCenter:SingleCell|Single Cell]] and [[BioMicroCenter:SpTx|Spatial]]&amp;lt;/B&amp;gt;&amp;lt;/BIG&amp;gt;&lt;br /&gt;
* 10X CHROMIUM &amp;lt;BR&amp;gt; &amp;lt;small&amp;gt; (5&#039;RNA, 3&#039;RNA, ATAC, multiome) &amp;lt;/small&amp;gt;&lt;br /&gt;
* 10X VISIUM &amp;lt;BR&amp;gt; &amp;lt;small&amp;gt; (Standard, High Density) &amp;lt;/small&amp;gt;&lt;br /&gt;
* ELEMENT AVITI24 (&amp;lt;i&amp;gt;in situ&amp;lt;/i&amp;gt; prep/sequencing)&lt;br /&gt;
 |}&lt;br /&gt;
 |valign=&amp;quot;top&amp;quot; align=&amp;quot;center&amp;quot; style=&amp;quot;background-color:transparent; border:1px solid black&amp;quot;| &lt;br /&gt;
 {|&lt;br /&gt;
  |width=&amp;quot;10%&amp;quot; align=&amp;quot;left&amp;quot;|&amp;lt;BIG&amp;gt;&amp;lt;B&amp;gt; [[BioMicroCenter:Sequencing|Sequencing Technologies]] &amp;lt;/B&amp;gt;&amp;lt;/BIG&amp;gt;&lt;br /&gt;
* High Yield (&amp;gt;1b reads) &amp;lt;small&amp;gt;&lt;br /&gt;
**[[BioMicroCenter:Illumina_Sequencing|Illumina NovaSeqX (25b,10b)]]†&amp;lt;/small&amp;gt;&lt;br /&gt;
* Mid Yield (250m-1b reads) &amp;lt;small&amp;gt;&lt;br /&gt;
**[[BioMicroCenter:Element Sequencing|Element AVITI24]] &amp;lt;/small&amp;gt;&lt;br /&gt;
* Low Yield (1m-150m reads) &amp;lt;small&amp;gt;&lt;br /&gt;
**[[BioMicroCenter:Singular Sequencing|SingularG4]]&lt;br /&gt;
**[[BioMicroCenter:Illumina_Sequencing|Illumina MiSeq i100]]&amp;lt;/small&amp;gt;&lt;br /&gt;
* Long Read (1kb+) &amp;lt;small&amp;gt;&lt;br /&gt;
**[[BioMicroCenter:Oxford Nanopore Technologies|ONT PromethION]] &lt;br /&gt;
**[[BioMicroCenter:PacBio|PacBio Revio]]†&amp;lt;/small&amp;gt;&lt;br /&gt;
 |}&lt;br /&gt;
 |valign=&amp;quot;top&amp;quot; align=&amp;quot;center&amp;quot; style=&amp;quot;background-color:transparent; border:1px solid black&amp;quot;| &lt;br /&gt;
 {|&lt;br /&gt;
  |width=&amp;quot;10%&amp;quot; align=&amp;quot;left&amp;quot;|&amp;lt;BIG&amp;gt;&amp;lt;B&amp;gt;Automation / Library Prep&amp;lt;/B&amp;gt;&amp;lt;/BIG&amp;gt;&lt;br /&gt;
* [[BioMicroCenter:Tecan Freedom Evo|Liquid Handlers]] ([[BioMicroCenter:Tecan_Freedom_Evo#JOHNNY_5|Tecan EVO]]&lt;br /&gt;
* [[BioMicroCenter:Tecan_Freedom_Evo#SPT_LABTECH_MOSQUITO_HV|SPT Mosquito]])&lt;br /&gt;
* [[BioMicroCenter:Covaris|Covaris E220 and R230 Focused-ultrasonicators]]&lt;br /&gt;
* [[BioMicroCenter:PippinPrep|Pippin Prep automated electrophoresis system]]&lt;br /&gt;
* [[BioMicroCenter:Oligo_Synthesis| Oligo Synthesis]]&lt;br /&gt;
 |}&lt;br /&gt;
 |- &lt;br /&gt;
 |valign=&amp;quot;top&amp;quot; colspan=3 align=&amp;quot;center&amp;quot; style=&amp;quot;background-color: lightsalmon; border:1px solid black&amp;quot;| &lt;br /&gt;
 {|&lt;br /&gt;
  |&amp;lt;BIG&amp;gt;&amp;lt;B&amp;gt;Informatics Services&amp;lt;/B&amp;gt;&amp;lt;/BIG&amp;gt;&lt;br /&gt;
 |}&lt;br /&gt;
 |-&lt;br /&gt;
 |valign=&amp;quot;top&amp;quot; align=&amp;quot;center&amp;quot; style=&amp;quot;background-color:transparent; border:1px solid black&amp;quot;| &lt;br /&gt;
 {|&lt;br /&gt;
  |width=&amp;quot;10%&amp;quot; align=&amp;quot;left&amp;quot;|&amp;lt;BIG&amp;gt;&amp;lt;B&amp;gt;[[BioMicroCenter:Analysis|Data Analysis]]&amp;lt;/B&amp;gt;&amp;lt;/BIG&amp;gt;&lt;br /&gt;
* [https://igb.mit.edu/ Bioinformatic Consulting]&lt;br /&gt;
* [[BioMicroCenter:People#INFORMATICS_LAB|Informatics Staff]]&lt;br /&gt;
 |}&lt;br /&gt;
 |valign=&amp;quot;top&amp;quot; align=&amp;quot;center&amp;quot; style=&amp;quot;background-color:transparent; border:1px solid black&amp;quot;| &lt;br /&gt;
 {|&lt;br /&gt;
  |width=&amp;quot;10%&amp;quot; align=&amp;quot;left&amp;quot;|&amp;lt;BIG&amp;gt;&amp;lt;B&amp;gt;BioIT/Research Computing&amp;lt;/B&amp;gt;&amp;lt;/BIG&amp;gt;&lt;br /&gt;
* [https://koch-institute-mit.gitbook.io/mit-data-management-analysis-core/ FAIR Data Management]&lt;br /&gt;
* [https://igb.mit.edu/computing-resources/active-data-storage Data Storage]&lt;br /&gt;
* [https://igb.mit.edu/computing-resources/luria-cluster Computing on Luria]&lt;br /&gt;
* [https://igb.mit.edu/data-management/globus Data Transfer (Globus)]&lt;br /&gt;
 |}&lt;br /&gt;
 |valign=&amp;quot;top&amp;quot; align=&amp;quot;center&amp;quot; style=&amp;quot;background-color:transparent; border:1px solid black&amp;quot;| &lt;br /&gt;
 {|&lt;br /&gt;
  |width=&amp;quot;10%&amp;quot; align=&amp;quot;left&amp;quot;|&amp;lt;BIG&amp;gt;&amp;lt;B&amp;gt;Education/Training/Seminars&amp;lt;/B&amp;gt;&amp;lt;/BIG&amp;gt;&lt;br /&gt;
* [https://igb.mit.edu/mini-courses Informatics/Computing Training Sessions]&lt;br /&gt;
* [[BioMicroCenter:Technology_Seminar_Series|Technology Seminar Series]]&lt;br /&gt;
* [http://mailman.mit.edu:/mailman/listinfo/biostuff Biostuff mailing list]&lt;br /&gt;
 |}&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
† - service will be performed in a sister core facility (Harvard, DFCI, or Broad)&amp;lt;BR&amp;gt;&lt;br /&gt;
&amp;lt;BR&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
[https://wikis.mit.edu/confluence/display/bmc &#039;&#039;BMC Internal wiki&#039;&#039;]&lt;br /&gt;
&lt;br /&gt;
&amp;lt;h2 align=&amp;quot;center&amp;quot;&amp;gt; CONTACT US &amp;lt;/h2&amp;gt;&lt;br /&gt;
[http://whereis.mit.edu 68-322]&amp;lt;BR&amp;gt;&lt;br /&gt;
biomicro@mit.edu&amp;lt;BR&amp;gt;&lt;br /&gt;
617-715-4533&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=BioMicroCenter&amp;diff=131254</id>
		<title>BioMicroCenter</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=BioMicroCenter&amp;diff=131254"/>
		<updated>2026-04-14T17:22:43Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;&amp;lt;h2 align=&amp;quot;center&amp;quot;&amp;gt; Welcome to the MIT BioMicro Center! &amp;lt;/h2&amp;gt;&lt;br /&gt;
&lt;br /&gt;
{| width=&amp;quot;100%&amp;quot; &lt;br /&gt;
 |- &lt;br /&gt;
 |valign=&amp;quot;top&amp;quot;|&lt;br /&gt;
[[File:logo.png|left]]&lt;br /&gt;
 | valign=&amp;quot;top&amp;quot; align=&amp;quot;left&amp;quot;|&lt;br /&gt;
* [[BioMicroCenter:News|&#039;&#039;&#039;News &amp;amp; General Information&#039;&#039;&#039;]]&lt;br /&gt;
* [[BioMicroCenter:Pricing|Pricing]] [[MIT:Pricing|MIT Pricing]]&lt;br /&gt;
* [[BioMicroCenter:Submission|Sample Submission]]&lt;br /&gt;
* [[BioMicroCenter:Consumables|Consumables]]&lt;br /&gt;
 | valign=&amp;quot;top&amp;quot; align=&amp;quot;left&amp;quot;|&lt;br /&gt;
* [[BioMicroCenter:AboutBMC|About the BioMicro Center]]&lt;br /&gt;
* [[BioMicroCenter:People| Staff]] &lt;br /&gt;
&amp;lt;!-- [[BioMicroCenter:Jobs|Positions Available]] --&amp;gt;&lt;br /&gt;
* [[BioMicroCenter:FAQ|FAQs]]&lt;br /&gt;
|}&lt;br /&gt;
The &#039;&#039;&#039;MIT BioMicro Center&#039;&#039;&#039; is an integrated genomics core facility that provides both expertise and equipment for systems biology. The core has significant resources in sample preparation, next generation sequencing and in high throughput screening as well as bioinformatics and BioIT. The Center is a joint endeavor between the [http://biology.mit.edu Department of Biology], the [http://ki.mit.edu Koch Institute for Integrative Cancer Research] (Integrated Genomics and Bioinformatics Core (IGB)), and the [http://web.mit.edu/be Department of Biological Engineering].&lt;br /&gt;
&lt;br /&gt;
{| width=&amp;quot;100%&amp;quot;&lt;br /&gt;
 |- valign=&amp;quot;top&amp;quot;&lt;br /&gt;
 |colspan=2 align=&amp;quot;center&amp;quot; style=&amp;quot;background-color:yellow; border:1px solid purple&amp;quot;| &lt;br /&gt;
 {|&lt;br /&gt;
  |&amp;lt;BIG&amp;gt;&amp;lt;B&amp;gt;[[BioMicroCenter:Assisted_Services|Assisted Services]] &amp;lt;/B&amp;gt;&amp;lt;/BIG&amp;gt;&lt;br /&gt;
 |}&lt;br /&gt;
 |align=&amp;quot;center&amp;quot; style=&amp;quot;background-color:aqua; border:1px solid black&amp;quot;|&lt;br /&gt;
 {|&lt;br /&gt;
  |&amp;lt;BIG&amp;gt;&amp;lt;B&amp;gt; Walkup Services &amp;lt;/B&amp;gt;&amp;lt;/BIG&amp;gt;&lt;br /&gt;
 |}&lt;br /&gt;
&lt;br /&gt;
 |-&lt;br /&gt;
 |valign=&amp;quot;top&amp;quot; align=&amp;quot;center&amp;quot; style=&amp;quot;background-color:transparent; border:1px solid black&amp;quot;| &lt;br /&gt;
 {|&lt;br /&gt;
  |width=&amp;quot;10%&amp;quot; align=&amp;quot;left&amp;quot;|&amp;lt;BIG&amp;gt;&amp;lt;B&amp;gt;Sample Isolation&amp;lt;/B&amp;gt;&amp;lt;/BIG&amp;gt;&lt;br /&gt;
* Sonication ([[BioMicroCenter:Covaris|Covaris E220/R230]])&lt;br /&gt;
* DNA/RNA purification ([[BioMicroCenter:Tecan_Freedom_Evo#Revvity_Chemagic_360|Chemagic360]])&lt;br /&gt;
* Quality Control&amp;lt;BR&amp;gt;([[BioMicroCenter:QC|AATI Fragment Analyzer/FemtoPulse]])&lt;br /&gt;
 |}&lt;br /&gt;
 |valign=&amp;quot;top&amp;quot; align=&amp;quot;center&amp;quot; style=&amp;quot;background-color:transparent; border:1px solid black&amp;quot;| &lt;br /&gt;
 {|&lt;br /&gt;
  |width=&amp;quot;10%&amp;quot; align=&amp;quot;left&amp;quot;|&amp;lt;BIG&amp;gt;&amp;lt;B&amp;gt;Library Preparation&amp;lt;/B&amp;gt;&amp;lt;/BIG&amp;gt;&lt;br /&gt;
* [[BioMicroCenter:Illumina_Library_Preparation|Short Read Sample Preparation]]&amp;lt;small&amp;gt;&lt;br /&gt;
** Standard Methods for [[BioMicroCenter:DNA_LIB| *DNA* ]] [[BioMicroCenter:RNA_LIB| *RNA* ]]&lt;br /&gt;
** [[BioMicroCenter:FAQ#STANDARD_versus_HIGH_THROUGHPUT_LIBRARY_PREPARATION|High Throughput Methods]] for [[BioMicroCenter:DNA_HTL| *DNA* ]]  [[BioMicroCenter:RNA_HTL| *RNA* ]]&amp;lt;/small&amp;gt;&lt;br /&gt;
* Long Read Sample Preparation&amp;lt;BR&amp;gt;([[BioMicroCenter:NanoPore_Library_Prep|Nanopore]] and [[BioMicroCenter:PacBio_Library_Preparation|PacBio]])&lt;br /&gt;
 |}&lt;br /&gt;
|valign=&amp;quot;top&amp;quot; align=&amp;quot;center&amp;quot; style=&amp;quot;background-color:transparent; border:1px solid black&amp;quot;| &lt;br /&gt;
 {|&lt;br /&gt;
  |width=&amp;quot;10%&amp;quot; align=&amp;quot;left&amp;quot;|&amp;lt;BIG&amp;gt;&amp;lt;B&amp;gt;[[BioMicroCenter:Walkup Services|Walk-up Instrumentation]]&amp;lt;/B&amp;gt;&amp;lt;/BIG&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;Includes: qPCR (LC480), Plate Readers (Varioskan/Synergy H1)&amp;lt;BR&amp;gt; BioAnalyzer, NanoDrop, Covaris, Pippin Prep&amp;lt;BR&amp;gt; Tecan/Chemagic automation&amp;lt;BR&amp;gt; MiSeq i100, 10x Chromium X&amp;lt;/small&amp;gt;&lt;br /&gt;
 |}&lt;br /&gt;
&lt;br /&gt;
 |-&lt;br /&gt;
 |valign=&amp;quot;top&amp;quot; align=&amp;quot;center&amp;quot; style=&amp;quot;background-color:transparent; border:1px solid black&amp;quot;| &lt;br /&gt;
 {|&lt;br /&gt;
  |width=&amp;quot;10%&amp;quot; align=&amp;quot;left&amp;quot;|&amp;lt;BIG&amp;gt;&amp;lt;B&amp;gt; &lt;br /&gt;
[[BioMicroCenter:SingleCell|Single Cell]] and [[BioMicroCenter:SpTx|Spatial]]&amp;lt;/B&amp;gt;&amp;lt;/BIG&amp;gt;&lt;br /&gt;
* 10X CHROMIUM &amp;lt;BR&amp;gt; &amp;lt;small&amp;gt; (5&#039;RNA, 3&#039;RNA, ATAC, multiome) &amp;lt;/small&amp;gt;&lt;br /&gt;
* 10X VISIUM &amp;lt;BR&amp;gt; &amp;lt;small&amp;gt; (Standard, High Density) &amp;lt;/small&amp;gt;&lt;br /&gt;
* ELEMENT AVITI24 (&amp;lt;i&amp;gt;in situ&amp;lt;/i&amp;gt; prep/sequencing)&lt;br /&gt;
 |}&lt;br /&gt;
 |valign=&amp;quot;top&amp;quot; align=&amp;quot;center&amp;quot; style=&amp;quot;background-color:transparent; border:1px solid black&amp;quot;| &lt;br /&gt;
 {|&lt;br /&gt;
  |width=&amp;quot;10%&amp;quot; align=&amp;quot;left&amp;quot;|&amp;lt;BIG&amp;gt;&amp;lt;B&amp;gt; [[BioMicroCenter:Sequencing|Sequencing Technologies]] &amp;lt;/B&amp;gt;&amp;lt;/BIG&amp;gt;&lt;br /&gt;
* High Yield (&amp;gt;1b reads) &amp;lt;small&amp;gt;&lt;br /&gt;
**[[BioMicroCenter:Illumina_Sequencing|Illumina NovaSeqX (25b,10b)]]†&amp;lt;/small&amp;gt;&lt;br /&gt;
* Mid Yield (250m-1b reads) &amp;lt;small&amp;gt;&lt;br /&gt;
**[[BioMicroCenter:Element Sequencing|Element AVITI24]] &amp;lt;/small&amp;gt;&lt;br /&gt;
* Low Yield (1m-150m reads) &amp;lt;small&amp;gt;&lt;br /&gt;
**[[BioMicroCenter:Singular Sequencing|SingularG4]]&lt;br /&gt;
**[[BioMicroCenter:Illumina_Sequencing|Illumina MiSeq i100]]&amp;lt;/small&amp;gt;&lt;br /&gt;
* Long Read (1kb+) &amp;lt;small&amp;gt;&lt;br /&gt;
**[[BioMicroCenter:Oxford Nanopore Technologies|ONT PromethION]] &lt;br /&gt;
**[[BioMicroCenter:PacBio|PacBio Revio]]†&amp;lt;/small&amp;gt;&lt;br /&gt;
 |}&lt;br /&gt;
 |valign=&amp;quot;top&amp;quot; align=&amp;quot;center&amp;quot; style=&amp;quot;background-color:transparent; border:1px solid black&amp;quot;| &lt;br /&gt;
 {|&lt;br /&gt;
  |width=&amp;quot;10%&amp;quot; align=&amp;quot;left&amp;quot;|&amp;lt;BIG&amp;gt;&amp;lt;B&amp;gt;Automation / Library Prep&amp;lt;/B&amp;gt;&amp;lt;/BIG&amp;gt;&lt;br /&gt;
* [[BioMicroCenter:Tecan Freedom Evo|Liquid Handlers]] ([[BioMicroCenter:Tecan_Freedom_Evo#JOHNNY_5|Tecan EVO]]&lt;br /&gt;
* [[BioMicroCenter:Tecan_Freedom_Evo#SPT_LABTECH_MOSQUITO_HV|SPT Mosquito]])&lt;br /&gt;
* [[BioMicroCenter:Covaris|Covaris E220 and R230 Focused-ultrasonicators]]&lt;br /&gt;
* [[BioMicroCenter:PippinPrep|Pippin Prep automated electrophoresis system]]&lt;br /&gt;
* [[BioMicroCenter:Oligo_Synthesis| Oligo Synthesis]]&lt;br /&gt;
 |}&lt;br /&gt;
 |- &lt;br /&gt;
 |valign=&amp;quot;top&amp;quot; colspan=3 align=&amp;quot;center&amp;quot; style=&amp;quot;background-color: lightsalmon; border:1px solid black&amp;quot;| &lt;br /&gt;
 {|&lt;br /&gt;
  |&amp;lt;BIG&amp;gt;&amp;lt;B&amp;gt;Informatics Services&amp;lt;/B&amp;gt;&amp;lt;/BIG&amp;gt;&lt;br /&gt;
 |}&lt;br /&gt;
 |-&lt;br /&gt;
 |valign=&amp;quot;top&amp;quot; align=&amp;quot;center&amp;quot; style=&amp;quot;background-color:transparent; border:1px solid black&amp;quot;| &lt;br /&gt;
 {|&lt;br /&gt;
  |width=&amp;quot;10%&amp;quot; align=&amp;quot;left&amp;quot;|&amp;lt;BIG&amp;gt;&amp;lt;B&amp;gt;[[BioMicroCenter:Analysis|Data Analysis]]&amp;lt;/B&amp;gt;&amp;lt;/BIG&amp;gt;&lt;br /&gt;
* [https://igb.mit.edu/ Bioinformatic Consulting]&lt;br /&gt;
* [[BioMicroCenter:People#INFORMATICS_LAB|Informatics Staff]]&lt;br /&gt;
 |}&lt;br /&gt;
 |valign=&amp;quot;top&amp;quot; align=&amp;quot;center&amp;quot; style=&amp;quot;background-color:transparent; border:1px solid black&amp;quot;| &lt;br /&gt;
 {|&lt;br /&gt;
  |width=&amp;quot;10%&amp;quot; align=&amp;quot;left&amp;quot;|&amp;lt;BIG&amp;gt;&amp;lt;B&amp;gt;BioIT/Research Computing&amp;lt;/B&amp;gt;&amp;lt;/BIG&amp;gt;&lt;br /&gt;
* [https://koch-institute-mit.gitbook.io/mit-data-management-analysis-core/ FAIR Data Management]&lt;br /&gt;
* [https://igb.mit.edu/computing-resources/active-data-storage Data Storage]&lt;br /&gt;
* [https://igb.mit.edu/computing-resources/luria-cluster Computing on Luria]&lt;br /&gt;
* [https://igb.mit.edu/data-management/globus Data Transfer (Globus)]&lt;br /&gt;
 |}&lt;br /&gt;
 |valign=&amp;quot;top&amp;quot; align=&amp;quot;center&amp;quot; style=&amp;quot;background-color:transparent; border:1px solid black&amp;quot;| &lt;br /&gt;
 {|&lt;br /&gt;
  |width=&amp;quot;10%&amp;quot; align=&amp;quot;left&amp;quot;|&amp;lt;BIG&amp;gt;&amp;lt;B&amp;gt;Education/Training/Seminars&amp;lt;/B&amp;gt;&amp;lt;/BIG&amp;gt;&lt;br /&gt;
* [https://igb.mit.edu/mini-courses Informatics/Computing Training Sessions]&lt;br /&gt;
* [[BioMicroCenter:Technology_Seminar_Series|Technology Seminar Series]]&lt;br /&gt;
* [http://mailman.mit.edu:/mailman/listinfo/biostuff Biostuff mailing list]&lt;br /&gt;
 |}&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
† - service will be performed in a sister core facility (Harvard, DFCI, or Broad)&amp;lt;BR&amp;gt;&lt;br /&gt;
&amp;lt;BR&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
[https://wikis.mit.edu/confluence/display/bmc &#039;&#039;BMC Internal wiki&#039;&#039;]&lt;br /&gt;
&lt;br /&gt;
&amp;lt;h2 align=&amp;quot;center&amp;quot;&amp;gt; CONTACT US &amp;lt;/h2&amp;gt;&lt;br /&gt;
[http://whereis.mit.edu 68-322]&amp;lt;BR&amp;gt;&lt;br /&gt;
biomicro@mit.edu&amp;lt;BR&amp;gt;&lt;br /&gt;
617-715-4533&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:Analysis&amp;diff=131250</id>
		<title>BioMicroCenter:Analysis</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:Analysis&amp;diff=131250"/>
		<updated>2026-04-14T15:50:45Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: Create Analysis page: bioinformatics services, staff, training, acknowledgment&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;{{BioMicroCenter}}&lt;br /&gt;
&lt;br /&gt;
The MIT BioMicro Center bioinformatics group provides data analysis support for members of the Departments of Biology and Biological Engineering. This is the same team and resource that serves Koch Institute members under the name [https://ki-sbc.mit.edu/dsmi Data Science &amp;amp; Multimodal Integration (DSMI)] — access, staff, and capabilities are identical regardless of which department you belong to.&lt;br /&gt;
&lt;br /&gt;
== BIOINFORMATICS SERVICES ==&lt;br /&gt;
&lt;br /&gt;
The BioMicro Center offers project-based bioinformatics collaboration. Staff work directly with researchers to plan and execute analyses, document their work to support manuscript preparation, and check in regularly to keep projects on track. Projects may be paused at any time by the researcher.&lt;br /&gt;
&lt;br /&gt;
To start a project, submit a request through [https://mit.ilabsolutions.com/sc/3381/ki-genomics-core-mit-biomicro-center/ iLabs] with a brief description of your experiment. A staff member will contact you to arrange a free initial consultation.&lt;br /&gt;
&lt;br /&gt;
=== Methods ===&lt;br /&gt;
&lt;br /&gt;
The informatics team works across a broad range of genomic and multi-omic methods. The following is a sampling of areas in which the group has active experience:&lt;br /&gt;
&lt;br /&gt;
* Transcriptomics (bulk RNA-Seq, single-cell RNA-Seq, spatial transcriptomics)&lt;br /&gt;
* Regulatory genomics (ChIP-Seq, ATAC-Seq, CUT&amp;amp;RUN, Hi-C)&lt;br /&gt;
* Variant analysis (SNP calling, copy number variation, whole-genome sequencing)&lt;br /&gt;
* Metagenomics&lt;br /&gt;
* Functional analyses (gene set enrichment, pathway analysis, WGCNA)&lt;br /&gt;
* Machine learning, deep learning, and generative AI applications&lt;br /&gt;
* Custom analysis pipelines and manuscript data portals&lt;br /&gt;
&lt;br /&gt;
=== Pricing ===&lt;br /&gt;
&lt;br /&gt;
Bioinformatics support is available either hourly or as a fractional FTE. Labs that purchase a fractional FTE receive priority access; one unit equals 10% of an FTE, approximately 4 hours per week, measured quarterly. Full pricing is on the [[BioMicroCenter:Pricing|Pricing page]].&lt;br /&gt;
&lt;br /&gt;
== BIOINFORMATICS STAFF ==&lt;br /&gt;
&lt;br /&gt;
[[User:Stuart S. Levine|Stuart Levine, PhD]] is the director of the BioMicro Center (slevine@mit.edu) and [https://scholar.google.com/citations?user=c3eio1wAAAAJ Google Scholar].&lt;br /&gt;
&lt;br /&gt;
[https://ki.mit.edu/people/sbc-leadership/charlie-whittaker Charlie Whittaker, PhD] is the Facility Scientific Director (charliew@mit.edu) and [https://scholar.google.com/scholar?q=Charles+A+Whittaker+MIT+bioinformatics Google Scholar].&lt;br /&gt;
&lt;br /&gt;
Vincent Butty, MD, PhD is a Bioinformatics Research Scientist (vbutty@mit.edu) and [https://scholar.google.com/citations?user=OiMbt6cAAAAJ Google Scholar].&lt;br /&gt;
&lt;br /&gt;
Duan Ma, PhD is a Bioinformatics Research Scientist (duan@mit.edu) and [https://scholar.google.com/scholar?q=Duanduan+Ma+MIT+bioinformatics Google Scholar].&lt;br /&gt;
&lt;br /&gt;
Yann Vanrobaeys, PhD is a Bioinformatics Research Scientist (yannvrb@mit.edu) and [https://scholar.google.ca/citations?user=JJPt-ZsAAAAJ Google Scholar].&lt;br /&gt;
&lt;br /&gt;
Huiming Ding, PhD is a Bioinformatics Research Scientist (huiming@mit.edu) and [https://scholar.google.com/scholar?q=Huiming+Ding+MIT+bioinformatics Google Scholar].&lt;br /&gt;
&lt;br /&gt;
== TRAINING ==&lt;br /&gt;
&amp;lt;!-- Link to BioinfoClasses page --&amp;gt;&lt;br /&gt;
Monthly training sessions are offered on a broad range of computational topics. Sessions are advertised by email; registration is through [https://mit.ilabsolutions.com/sc/3381/ki-genomics-core-mit-biomicro-center/ iLabs]. Topics include UNIX and cluster usage, R and Python, RNA-Seq and scRNA-Seq analysis, FAIR data principles, and machine learning. Custom sessions for individuals or small groups can be arranged by contacting [mailto:biomicro@mit.edu biomicro@mit.edu]. See [[BioMicroCenter:BioinfoClasses|the Classes page]] for the current schedule.&lt;br /&gt;
&lt;br /&gt;
== ACKNOWLEDGMENT AND AUTHORSHIP ==&lt;br /&gt;
&lt;br /&gt;
It is important to acknowledge use of the BioMicro Center in your publications. Suggested language is available on the [https://ki-sbc.mit.edu/acknowledge Koch Institute acknowledgment page].&lt;br /&gt;
&lt;br /&gt;
When a member of the Core has contributed significantly to a project, we ask that you consider making them a co-author. BioMicro Center staff are valued scientists and their contributions deserve recognition like any other co-author. For guidelines on including Core Facility personnel as co-authors, please see the [https://www.abrf.org/authorship-guidelines recommendations of the Association of Biomolecular Resource Facilities (ABRF)].&lt;br /&gt;
&lt;br /&gt;
== OTHER SERVICES ==&lt;br /&gt;
&lt;br /&gt;
* &#039;&#039;&#039;Research computing&#039;&#039;&#039; — storage (BMC-LAB), the LURIA compute cluster, and Project AMON at MGHPCC: [[BioMicroCenter:Servers|Research Computing page]]&lt;br /&gt;
* &#039;&#039;&#039;Data management&#039;&#039;&#039; — repository submission, FAIR compliance, and archiving: [[BioMicroCenter:DataManagement|Data Management page]]&lt;br /&gt;
&lt;br /&gt;
[[Category:BioMicroCenter]]&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:Consumables&amp;diff=131246</id>
		<title>BioMicroCenter:Consumables</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:Consumables&amp;diff=131246"/>
		<updated>2026-04-13T16:53:42Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: Create Consumables page: bulk reagents, supplies, and MiSeq i100 cartridges&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;{{BioMicroCenter}}&lt;br /&gt;
&lt;br /&gt;
== Consumables ==&lt;br /&gt;
&lt;br /&gt;
The BioMicro Center purchases reagents and supplies in bulk, passing the discount on to users. Items can be requested directly through iLabs — no need to source them independently.&lt;br /&gt;
&lt;br /&gt;
To place an order, log in to [https://mit-ki.ilabsolutions.com/sc/3381/ki-genomics-core-mit-biomicro-center/?tab=services iLabs] and navigate to &#039;&#039;&#039;Request Services → BioMicro Requests → BioMicro Center Consumables → Initiate Request&#039;&#039;&#039;.&lt;br /&gt;
&lt;br /&gt;
If you need something not on the list, contact [mailto:biomicro@mit.edu biomicro@mit.edu] directly.&lt;br /&gt;
&lt;br /&gt;
----&lt;br /&gt;
&lt;br /&gt;
== Available Items ==&lt;br /&gt;
&lt;br /&gt;
=== Reagents and Supplies ===&lt;br /&gt;
&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot;&lt;br /&gt;
! Item !! Unit Price (MIT-BMC-CORE)&lt;br /&gt;
|-&lt;br /&gt;
| Reagent: SYBR Green (Roche / Kapa) || $45.00 / mL&lt;br /&gt;
|-&lt;br /&gt;
| Plates, 96-well || $10.00 / plate&lt;br /&gt;
|-&lt;br /&gt;
| Plates, 384-well || $12.00 / plate&lt;br /&gt;
|-&lt;br /&gt;
| Reagent: Ampure Beads || $11.00 / mL&lt;br /&gt;
|-&lt;br /&gt;
| Reagent: RNAClean Beads || $20.00 / mL&lt;br /&gt;
|-&lt;br /&gt;
| Illumina Oligo Aliquot || $4.00 / 5 µL&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
=== [[BioMicroCenter:Walkup Services|MiSeq i100]] Sequencing Cartridges ===&lt;br /&gt;
&lt;br /&gt;
These cartridges are purchased here and used on the walk-up MiSeq i100. See [[BioMicroCenter:Walkup Services|Walk-up Services]] for scheduling.&lt;br /&gt;
&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot;&lt;br /&gt;
! Cartridge !! Reads !! Unit Price (MIT-BMC-CORE)&lt;br /&gt;
|-&lt;br /&gt;
| MiSeq i100: 300nt || 5M || $381.00&lt;br /&gt;
|-&lt;br /&gt;
| MiSeq i100: 600nt || 5M || $785.00&lt;br /&gt;
|-&lt;br /&gt;
| MiSeq i100: 100nt || 25M || $565.00&lt;br /&gt;
|-&lt;br /&gt;
| MiSeq i100: 300nt || 25M || $920.00&lt;br /&gt;
|-&lt;br /&gt;
| MiSeq i100: 600nt || 25M || $1,125.00&lt;br /&gt;
|-&lt;br /&gt;
| MiSeq i100: 1Knt || 25M || $1,921.00&lt;br /&gt;
|-&lt;br /&gt;
| MiSeq i100: 100nt || 100M || $1,130.00&lt;br /&gt;
|-&lt;br /&gt;
| MiSeq i100: 300nt || 100M || $1,638.50&lt;br /&gt;
|-&lt;br /&gt;
| MiSeq i100: 600nt || 50M || $1,638.50&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
Prices shown are for MIT-BMC-CORE users. See [[BioMicroCenter:Pricing|Pricing]] for full rate tiers.&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:Assisted_Services&amp;diff=131244</id>
		<title>BioMicroCenter:Assisted Services</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:Assisted_Services&amp;diff=131244"/>
		<updated>2026-04-13T16:45:35Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: Create Assisted Services page: submission workflow, 6 service area cards&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;{{BioMicroCenter}}&lt;br /&gt;
&lt;br /&gt;
== Assisted Services ==&lt;br /&gt;
&lt;br /&gt;
Assisted services are those where samples are delivered to the BioMicro Center and processed by staff. This includes sample preparation, library preparation, and sequencing. For equipment you operate yourself, see [[BioMicroCenter:Walkup Services|Walk-up Services]].&lt;br /&gt;
&lt;br /&gt;
----&lt;br /&gt;
&lt;br /&gt;
== How to Submit ==&lt;br /&gt;
&lt;br /&gt;
=== Step 1: Choose your request form ===&lt;br /&gt;
&lt;br /&gt;
Log in to [https://mit-ki.ilabsolutions.com/sc/3381/ki-genomics-core-mit-biomicro-center/?tab=services iLabs] and navigate to &#039;&#039;&#039;Request Services → BioMicro Requests&#039;&#039;&#039;. Select the form appropriate for your project:&lt;br /&gt;
&lt;br /&gt;
* &#039;&#039;&#039;[[BioMicroCenter:QC|BioAnalyzer / Fragment Analyzer / FemtoPulse / qPCR]]&#039;&#039;&#039; — for quality control of DNA or RNA only (not paired with sequencing)&lt;br /&gt;
* &#039;&#039;&#039;[[BioMicroCenter:Illumina_Library_Preparation|DNA / RNA Library Prep and/or Short Read Sequencing]]&#039;&#039;&#039; — for samples to be made into short read libraries, and/or sequenced on Illumina MiSeq, NextSeq, NovaSeq, Singular G4, or Element AVITI24&lt;br /&gt;
* &#039;&#039;&#039;[[BioMicroCenter:SingleCell|Single Cell – 10X]]&#039;&#039;&#039; — for 10X Genomics single cell experiments (3′DGE, 5′DGE, ATACseq, CNV analysis)&lt;br /&gt;
* &#039;&#039;&#039;[[BioMicroCenter:Oxford_Nanopore_Technologies|Long Read Sequencing (Nanopore and PacBio)]]&#039;&#039;&#039; — for projects to be sequenced on the Oxford Nanopore PromethION P2Solo or PacBio Revio&lt;br /&gt;
* &#039;&#039;&#039;[[BioMicroCenter:SpTx|Spatial Omics]]&#039;&#039;&#039; — for 10X Visium HD and AVITI24 in situ sequencing experiments&lt;br /&gt;
* &#039;&#039;&#039;[[BioMicroCenter:Oligo_Synthesis|Oligo Synthesis]]&#039;&#039;&#039; — for full-plate oligonucleotide synthesis using the DNAscript system&lt;br /&gt;
&lt;br /&gt;
All projects submitted via iLabs &#039;&#039;&#039;must&#039;&#039;&#039; have a valid MIT cost object. If paying by purchase order, include the PO number in the form; the cost object serves as a backup.&lt;br /&gt;
&lt;br /&gt;
Submitting a form in iLabs does &#039;&#039;&#039;not&#039;&#039;&#039; begin your project. The project begins only after samples have been received and confirmed by BMC staff.&lt;br /&gt;
&lt;br /&gt;
=== Step 2: Bring your samples to the lab ===&lt;br /&gt;
&lt;br /&gt;
After submitting your iLabs form, bring your samples to &#039;&#039;&#039;68-322&#039;&#039;&#039; during regular business hours. Staff will review your form with you when you arrive, confirm your experimental requirements, and assign a BMC project ID.&lt;br /&gt;
&lt;br /&gt;
If you cannot access the building, call the lab at &#039;&#039;&#039;617-715-4533&#039;&#039;&#039;.&lt;br /&gt;
&lt;br /&gt;
For questions or to coordinate a drop-off time in advance, email [mailto:biomicro@mit.edu biomicro@mit.edu].&lt;br /&gt;
&lt;br /&gt;
----&lt;br /&gt;
&lt;br /&gt;
== Service Areas ==&lt;br /&gt;
&lt;br /&gt;
{| width=&amp;quot;100%&amp;quot;&lt;br /&gt;
|-&lt;br /&gt;
|valign=&amp;quot;top&amp;quot; align=&amp;quot;center&amp;quot; style=&amp;quot;background-color:transparent; border:1px solid black&amp;quot;|&lt;br /&gt;
{|&lt;br /&gt;
|width=&amp;quot;10%&amp;quot; align=&amp;quot;left&amp;quot;|&amp;lt;BIG&amp;gt;&amp;lt;B&amp;gt;[[BioMicroCenter:QC|Sample QC]]&amp;lt;/B&amp;gt;&amp;lt;/BIG&amp;gt;&lt;br /&gt;
Fragment Analyzer, FemtoPulse, BioAnalyzer 2100&lt;br /&gt;
|}&lt;br /&gt;
|valign=&amp;quot;top&amp;quot; align=&amp;quot;center&amp;quot; style=&amp;quot;background-color:transparent; border:1px solid black&amp;quot;|&lt;br /&gt;
{|&lt;br /&gt;
|width=&amp;quot;10%&amp;quot; align=&amp;quot;left&amp;quot;|&amp;lt;BIG&amp;gt;&amp;lt;B&amp;gt;[[BioMicroCenter:Illumina_Library_Preparation|Library Preparation]]&amp;lt;/B&amp;gt;&amp;lt;/BIG&amp;gt;&lt;br /&gt;
Short read (DNA &amp;amp; RNA), Long read (Nanopore, PacBio)&lt;br /&gt;
|}&lt;br /&gt;
|valign=&amp;quot;top&amp;quot; align=&amp;quot;center&amp;quot; style=&amp;quot;background-color:transparent; border:1px solid black&amp;quot;|&lt;br /&gt;
{|&lt;br /&gt;
|width=&amp;quot;10%&amp;quot; align=&amp;quot;left&amp;quot;|&amp;lt;BIG&amp;gt;&amp;lt;B&amp;gt;[[BioMicroCenter:Sequencing|Sequencing]]&amp;lt;/B&amp;gt;&amp;lt;/BIG&amp;gt;&lt;br /&gt;
NovaSeqX, AVITI24, Singular G4, MiSeq i100, Nanopore, PacBio&lt;br /&gt;
|}&lt;br /&gt;
|-&lt;br /&gt;
|valign=&amp;quot;top&amp;quot; align=&amp;quot;center&amp;quot; style=&amp;quot;background-color:transparent; border:1px solid black&amp;quot;|&lt;br /&gt;
{|&lt;br /&gt;
|width=&amp;quot;10%&amp;quot; align=&amp;quot;left&amp;quot;|&amp;lt;BIG&amp;gt;&amp;lt;B&amp;gt;[[BioMicroCenter:SingleCell|Single Cell]]&amp;lt;/B&amp;gt;&amp;lt;/BIG&amp;gt;&lt;br /&gt;
10X Chromium (3&#039;DGE, 5&#039;DGE, ATACseq, multiome)&lt;br /&gt;
|}&lt;br /&gt;
|valign=&amp;quot;top&amp;quot; align=&amp;quot;center&amp;quot; style=&amp;quot;background-color:transparent; border:1px solid black&amp;quot;|&lt;br /&gt;
{|&lt;br /&gt;
|width=&amp;quot;10%&amp;quot; align=&amp;quot;left&amp;quot;|&amp;lt;BIG&amp;gt;&amp;lt;B&amp;gt;[[BioMicroCenter:SpTx|Spatial Omics]]&amp;lt;/B&amp;gt;&amp;lt;/BIG&amp;gt;&lt;br /&gt;
10X Visium HD, AVITI24 in situ sequencing&lt;br /&gt;
|}&lt;br /&gt;
|valign=&amp;quot;top&amp;quot; align=&amp;quot;center&amp;quot; style=&amp;quot;background-color:transparent; border:1px solid black&amp;quot;|&lt;br /&gt;
{|&lt;br /&gt;
|width=&amp;quot;10%&amp;quot; align=&amp;quot;left&amp;quot;|&amp;lt;BIG&amp;gt;&amp;lt;B&amp;gt;[[BioMicroCenter:Oligo_Synthesis|Oligo Synthesis]]&amp;lt;/B&amp;gt;&amp;lt;/BIG&amp;gt;&lt;br /&gt;
Full-plate synthesis via DNAscript&lt;br /&gt;
|}&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
----&lt;br /&gt;
&lt;br /&gt;
== Pricing ==&lt;br /&gt;
&lt;br /&gt;
See [[BioMicroCenter:Pricing|Pricing]] for current rates. MIT users must have an active cost object. External users should review the [[BioMicroCenter:Submission#External_Users|external user submission instructions]] and contact [mailto:biomicro@mit.edu biomicro@mit.edu] to arrange a project.&lt;br /&gt;
&lt;br /&gt;
----&lt;br /&gt;
&lt;br /&gt;
== Retrieving Your Data ==&lt;br /&gt;
&lt;br /&gt;
Once your data is ready, see [[BioMicroCenter:FAQ#DOWNLOADING_DATA|Downloading your data]] for instructions on accessing results.&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:Walkup_Services&amp;diff=131242</id>
		<title>BioMicroCenter:Walkup Services</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:Walkup_Services&amp;diff=131242"/>
		<updated>2026-04-13T01:57:42Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: Create Walkup Services overview page: scheduling, training, equipment index, policies&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;{{BioMicroCenter}}&lt;br /&gt;
&lt;br /&gt;
== What are walk-up services? ==&lt;br /&gt;
&lt;br /&gt;
Walk-up services are those where the BioMicro Center provides training, maintenance, and scheduling of equipment, and may also provide consumables. Users operate the instruments themselves after completing a required training session with BMC staff. Walk-up services are almost exclusively limited to MIT users; non-MIT users should contact [mailto:biomicro@mit.edu biomicro@mit.edu] to discuss access.&lt;br /&gt;
&lt;br /&gt;
Walk-up services contrast with [[BioMicroCenter:FAQ#ASSISTED_versus_WALKUP_SERVICES|assisted services]], where samples are delivered to BMC staff for processing. See [[BioMicroCenter:FAQ]] for a full definition of both service types.&lt;br /&gt;
&lt;br /&gt;
== Scheduling and iLabs ==&lt;br /&gt;
&lt;br /&gt;
All walk-up equipment is scheduled through [https://mit-ki.ilabsolutions.com/sc/3381/ki-genomics-core-mit-biomicro-center?tab=equipment iLabs]. The equipment calendar lists instruments organized by category (BMC:qPCR, BMC:Varioskan, BMC:BioAnalyzer, BMC:Covaris, etc.).&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;Note: Items listed as $0 in iLabs are charged per use — you will be asked to enter the number of units when submitting your reservation. The number of units is cross-validated in the lab.&#039;&#039;&lt;br /&gt;
&lt;br /&gt;
* Reservations must be made &#039;&#039;&#039;at least 4 hours in advance&#039;&#039;&#039;. For last-minute requests, email [mailto:biomicro@mit.edu biomicro@mit.edu].&lt;br /&gt;
* To arrange training, email [mailto:biomicro@mit.edu biomicro@mit.edu]. Depending on the instrument, staff will instruct you to book a time on the iLabs calendar or coordinate directly.&lt;br /&gt;
&lt;br /&gt;
== Training and access ==&lt;br /&gt;
&lt;br /&gt;
Training by BMC staff is required before using any walk-up instrument. Training sessions are typically one hour and cover instrument operation and best practices. To request training for any instrument, email [mailto:biomicro@mit.edu biomicro@mit.edu].&lt;br /&gt;
&lt;br /&gt;
Walk-up users may use equipment outside of regular business hours. After-hours access to the core requires a one-time sign-up — see the [[BioMicroCenter:Submission#Walk-up_services|Submission page]] for the after-hours access form.&lt;br /&gt;
&lt;br /&gt;
== Equipment available for walk-up ==&lt;br /&gt;
&lt;br /&gt;
Click any instrument name for full details, specifications, and scheduling links.&lt;br /&gt;
&lt;br /&gt;
=== Sample quantification and quality control ===&lt;br /&gt;
&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; style=&amp;quot;width:100%;&amp;quot;&lt;br /&gt;
|-&lt;br /&gt;
! style=&amp;quot;width:200px;&amp;quot; | Instrument !! Summary !! style=&amp;quot;width:110px;&amp;quot; | Schedule&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;[[BioMicroCenter:RTPCR|LightCycler 480 II (LC480)]]&#039;&#039;&#039;&lt;br /&gt;
| Real-time PCR for quantitative and qualitative detection of nucleic acids. Handles 96w and 384w plates; supports SYBR and TaqMan probes. Three units available (Thing 1 96w; Thing 2 &amp;amp; 3 384w). Maximum 4 hours/day during business hours (10 AM–5 PM); all slots available after hours. Donated by Prof. Laurie Boyer, Prof. Leonard Guarente, and the Dept. of Biology.&lt;br /&gt;
| [https://mit.ilabsolutions.com/equipment/show/261680/?tab=schedule iLabs]&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;[[BioMicroCenter:Varioskan|Varioskan Flash]]&#039;&#039;&#039;&lt;br /&gt;
| Multimode plate reader for fluorometric, luminometric, and absorbance measurements in 96/384w format. Equipped with three onboard dispensers, incubator, and orbital shaker. Charged in 10-minute blocks. Donated by Prof. Jeroen Saeij.&lt;br /&gt;
| [https://mit.ilabsolutions.com/equipment/show/262715/?tab=schedule iLabs]&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;[[BioMicroCenter:Varioskan#Synergy_H1|Synergy H1 plate reader]]&#039;&#039;&#039;&lt;br /&gt;
| Hybrid multi-mode microplate reader (6w–384w). Supports UV-vis measurements via Take3 Trio plate module (48 × 2 µL samples; Nanodrop-equivalent). Can be signed out for use &#039;&#039;outside&#039;&#039; the BMC — sign out one week in advance. Charged in 10-minute blocks. Donated by Prof. Gene-Wei Li.&lt;br /&gt;
| [https://mit.ilabsolutions.com/service_center/3381?tab=equipment iLabs]&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;[[BioMicroCenter:2100BioAnalyzer|Agilent 2100 BioAnalyzer]]&#039;&#039;&#039;&lt;br /&gt;
| Nanofluidic sizing and quantification of DNA (25 bp–12 kb), RNA, and protein. Users must provide all reagents — BMC does not stock BioAnalyzer chips or reagents.&lt;br /&gt;
| [https://mit-ki.ilabsolutions.com/equipment/show/265040/?tab=schedule iLabs]&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;[[BioMicroCenter:NanoDrop_ND-1000|NanoDrop ND-1000]]&#039;&#039;&#039;&lt;br /&gt;
| Full-spectrum spectrophotometer (220–750 nm) for 1 µL samples. Measures nucleic acid and protein concentration and purity. Free for core department users. Located in 68-316.&lt;br /&gt;
| No scheduling required&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
=== DNA/RNA sizing ===&lt;br /&gt;
&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; style=&amp;quot;width:100%;&amp;quot;&lt;br /&gt;
|-&lt;br /&gt;
! style=&amp;quot;width:200px;&amp;quot; | Instrument !! Summary !! style=&amp;quot;width:110px;&amp;quot; | Schedule&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;[[BioMicroCenter:PippinPrep|BluePippin / Pippin Prep]]&#039;&#039;&#039;&lt;br /&gt;
| Automated preparative gel electrophoresis for DNA size selection. BluePippin: 50 bp–50 kb; Pippin Prep: 100 bp–1.5 kb. Charged per run. Donated by the Department of Biology.&lt;br /&gt;
| [https://mit.ilabsolutions.com/equipment/show/262716/?tab=schedule iLabs]&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
=== Sonication ===&lt;br /&gt;
&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; style=&amp;quot;width:100%;&amp;quot;&lt;br /&gt;
|-&lt;br /&gt;
! style=&amp;quot;width:200px;&amp;quot; | Instrument !! Summary !! style=&amp;quot;width:110px;&amp;quot; | Schedule&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;[[BioMicroCenter:Covaris|Covaris E220 Evolution]]&#039;&#039;&#039;&lt;br /&gt;
| Focused ultrasonicator for single-tube processing (1–96 samples per batch). Processes 25 µL–10 mL sample volumes. Shears DNA, RNA, chromatin; homogenizes tissue.&lt;br /&gt;
| (see instrument page)&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;[[BioMicroCenter:Covaris|Covaris R230]]&#039;&#039;&#039;&lt;br /&gt;
| High-throughput sonicator for 96 AFA-TUBE® TPX plates. Column sonication mode processes an entire 96-well plate 10× faster than E220. Funded by NIH grant R24OD035444 — include in publications.&lt;br /&gt;
| (see instrument page)&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
=== Automation ===&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Note:&#039;&#039;&#039; All automation scripts must be written by BMC staff. Once a script has been programmed, users may run it independently as a walk-up service. Contact [mailto:biomicro@mit.edu biomicro@mit.edu] to initiate script development.&lt;br /&gt;
&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; style=&amp;quot;width:100%;&amp;quot;&lt;br /&gt;
|-&lt;br /&gt;
! style=&amp;quot;width:200px;&amp;quot; | Instrument !! Summary !! style=&amp;quot;width:110px;&amp;quot; | Schedule&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;[[BioMicroCenter:Tecan_Freedom_Evo#JOHNNY_5|Tecan EVO 150 — Johnny 5]]&#039;&#039;&#039;&lt;br /&gt;
| Liquid handler optimized for cherry picking and plate normalization. LiHa span8 (2–200 µL) + MCA96 (50/200 µL tips). 6× 15 mL tube carriers, 3 temperature-controlled + 24 non-temperature-controlled SBS carriers, UV decontamination. BL1 work only. Donated by the Department of Biology.&lt;br /&gt;
| [https://mit.ilabsolutions.com/equipment/show/262347/?tab=schedule iLabs]&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;[[BioMicroCenter:Tecan_Freedom_Evo#B9|Tecan EVO 150 — B9]]&#039;&#039;&#039;&lt;br /&gt;
| Liquid handler optimized for sample processing and assay setup. MCA96 + RoMa plate handler. 3 temperature-controlled + 24 non-temperature-controlled SBS carriers. BL1 work only.&lt;br /&gt;
| (see instrument page)&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;[[BioMicroCenter:Tecan_Freedom_Evo#Revvity_Chemagic_360|Revvity Chemagic 360]]&#039;&#039;&#039;&lt;br /&gt;
| Magnetic bead-based automated nucleic acid purification. 96-well EM rod head; 12-tip 3-row dispenser array. Processes 96 tissue or cell lysates in up to 5 hours. Charged by consumables. Requires setup steps (lysate preparation before loading). Contact [mailto:biomicro@mit.edu biomicro@mit.edu] at least one month in advance — purification-specific kits take approximately one month to arrive. Donated by the MIT Stem Cell Initiative and Dr. Jacqueline A. Lees.&lt;br /&gt;
| Contact BMC 1 month ahead&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
=== Sequencing ===&lt;br /&gt;
&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; style=&amp;quot;width:100%;&amp;quot;&lt;br /&gt;
|-&lt;br /&gt;
! style=&amp;quot;width:200px;&amp;quot; | Instrument !! Summary !! style=&amp;quot;width:110px;&amp;quot; | Schedule&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;[[BioMicroCenter:Illumina_Sequencing|Illumina MiSeq i100]]&#039;&#039;&#039;&lt;br /&gt;
| Walk-up short-read sequencing. A run charge must be added to each reservation (listed as $0 in iLabs but required). Reserve for the actual run duration: 100 nt = 5 h, 300 nt = 8 h, 600 nt = 16 h. Sequencing kits stocked by BMC; available during business hours as add-on charges or via the consumable request form. All data stored on BMC servers (BMC-PUB or BMC-LAB) — Illumina BaseSpace is not directly available from the instrument.&lt;br /&gt;
| (see iLabs equipment tab)&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
=== Single cell / spatial (walk-up option) ===&lt;br /&gt;
&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; style=&amp;quot;width:100%;&amp;quot;&lt;br /&gt;
|-&lt;br /&gt;
! style=&amp;quot;width:200px;&amp;quot; | Instrument !! Summary !! style=&amp;quot;width:110px;&amp;quot; | Schedule&lt;br /&gt;
|-&lt;br /&gt;
| &#039;&#039;&#039;[[BioMicroCenter:SingleCell|10x Chromium X]]&#039;&#039;&#039;&lt;br /&gt;
| Available as walk-up after training by BMC staff. Chips must be purchased through BMC. Reagents are also available through BMC. Contact [mailto:biomicro@mit.edu biomicro@mit.edu] to arrange training and initial access.&lt;br /&gt;
| (see iLabs equipment tab)&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
== Policies ==&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Training is mandatory.&#039;&#039;&#039; No use of any instrument is permitted without prior training by BMC staff. Untrained use will result in suspension of walk-up privileges for the entire laboratory.&lt;br /&gt;
&lt;br /&gt;
* BMC business has priority on all walk-up equipment at all times.&lt;br /&gt;
* Reservations must be made at least 4 hours in advance. Late requests should be emailed to [mailto:biomicro@mit.edu biomicro@mit.edu].&lt;br /&gt;
* Walk-up access is MIT only in almost all cases. Non-MIT users should contact [mailto:biomicro@mit.edu biomicro@mit.edu].&lt;br /&gt;
* For the MiSeq i100: violation of the access policy results in termination of walk-up privileges for the &#039;&#039;&#039;entire laboratory&#039;&#039;&#039;, not only the individual user.&lt;br /&gt;
* All automation scripts must be written by BMC staff before users may run them independently.&lt;br /&gt;
&lt;br /&gt;
== Getting help ==&lt;br /&gt;
&lt;br /&gt;
For general questions, training requests, or after-hours access:&lt;br /&gt;
&lt;br /&gt;
* &#039;&#039;&#039;Email:&#039;&#039;&#039; [mailto:biomicro@mit.edu biomicro@mit.edu]&lt;br /&gt;
* &#039;&#039;&#039;Phone:&#039;&#039;&#039; 617-715-4533&lt;br /&gt;
* &#039;&#039;&#039;After-hours access:&#039;&#039;&#039; [https://www.dropbox.com/scl/fi/nxdzbesvp2mab69wofotm/New_User_Sign_Up_Form.docx?rlkey=x9ftkatdlm897fbeol2ibv8tb&amp;amp;st=ag0jsvn9&amp;amp;dl=0 After-Hours Access Form] (one-time sign-up)&lt;br /&gt;
* &#039;&#039;&#039;Walk in:&#039;&#039;&#039; [http://whereis.mit.edu/?go=68 68-322, MIT campus]&lt;br /&gt;
&lt;br /&gt;
See also: [[BioMicroCenter:FAQ]] for definitions of walk-up vs. assisted services, core lab status, and billing.&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=MIT:Test&amp;diff=131241</id>
		<title>MIT:Test</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=MIT:Test&amp;diff=131241"/>
		<updated>2026-04-07T14:36:29Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: Created page with &amp;quot;Testing a new namespace for login-walled content&amp;quot;&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;Testing a new namespace for login-walled content&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:FAQ&amp;diff=131240</id>
		<title>BioMicroCenter:FAQ</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:FAQ&amp;diff=131240"/>
		<updated>2026-04-06T14:46:30Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;{{BioMicroCenter}}&lt;br /&gt;
&lt;br /&gt;
== CONTACT ==&lt;br /&gt;
&#039;&#039;&#039;STOP BY:&#039;&#039;&#039; The BioMicro Center is located at [http://whereis.mit.edu/?go=68 68-322 on the MIT campus]. &amp;lt;BR&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;EMAIL:&#039;&#039;&#039; The BioMicro [[BioMicroCenter:People|staff]] can be emailed at biomicro@mit.edu &amp;lt;BR&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;PHONE:&#039;&#039;&#039; The main lab number is 617-715-4533 &amp;lt;BR&amp;gt;&lt;br /&gt;
Our director, [[User:Stuart S. Levine|Stuart Levine]], can be reached at 617-452-2949.&lt;br /&gt;
&lt;br /&gt;
== DEFINITIONS ==&lt;br /&gt;
=== CORE LABS ===&lt;br /&gt;
The BioMicro Center is NOT an MIT institutional core, but a departmental resource. Departments that fund the operation of the BioMicro Center are given priority for all resources within the Center. Several labs that have provided major equipment donations to the BioMicro Center are also considered to be CORE. The BioMicro Center is funded by:&lt;br /&gt;
* The Department of Biology &lt;br /&gt;
* The Department of BioEngineering&lt;br /&gt;
* The David H. Koch Institute for Integrative Cancer Research&lt;br /&gt;
&lt;br /&gt;
=== ASSISTED versus WALKUP SERVICES ===&lt;br /&gt;
Services available in the BioMicro Center are offered either as assisted or as walk-up. Walk-up Services are those where the Center provides training and maintenance of equipment and may also provide some consumables. Scheduling for Walk-up services is available through the calendar functions on ilabs and are almost exclusively limited to MIT users except in rare cases. Non-MIT users should contact biomicro@mit.edu to schedule walk-up equipment.&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
Assisted services are those where samples are delivered to the BioMicro Center and are analyzed by Center staff. These are set up using the [[BioMicroCenter:Forms|FORMS]] on this site or the &amp;quot;Request Services&amp;quot; tab in [https://mit.ilabsolutions.com/sc/3381/ki-genomics-core-mit-biomicro-center/?tab=about ilabs]. Assisted services cannot typically be &amp;quot;scheduled&amp;quot; with rare exceptions.&lt;br /&gt;
&lt;br /&gt;
=== STANDARD versus HIGH THROUGHPUT LIBRARY PREPARATION ===&lt;br /&gt;
Standard library preparation is designed to maximize successful production of libraries and is used for routine work and should also be used for all precious samples. Standard library preparation includes initial sample quality control as well as at least one repeat for each library preparation if required due to sample failure. Standard library preparation is charged on a per sample basis. Standard libraries may be prepared by hand or using automation depending on throughput of the core. &amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
&lt;br /&gt;
High Throughput library (HTL) preparation is designed to minimize cost and has many differences from standard library preparation as a result. HTLs costs do NOT include initial sample quality control nor repeats of failures of individual samples. As such, we very strongly discourage HTL library prep for precious samples or experiments with limited replicates (N&amp;lt;=3). Replicate counts of 4 are considered the minimum with 6 being recommended where possible. Some methods, such as HT3DGE, can never repeat failed samples, while others can be reproduced by hand, though that does create a technical variable that can impact analysis. HTLs are almost always produced using liquid handlers, typically using miniaturization, and may require submission of the samples in specific formats (noted on each library prep type). HTLs are spot checked for quality after library production. Full quality control before and after experiments as well as sample arraying and sample cleaning is available at an additional charge.&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
&lt;br /&gt;
We have published a manuscript highlighting some of the methods on [https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/33100919/ JBT].&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
&lt;br /&gt;
A webinar by our director for SPT Labtech [https://www.sptlabtech.com/resources/automated-low-volume-liquid-handling-for-cost-effective-ngs-library-preparation-and-single-cell-genomics-webinar-sign-up can be found here]&lt;br /&gt;
&lt;br /&gt;
== DOWNLOADING DATA ==&lt;br /&gt;
You will be notified by email that your data is ready. The data will be placed on one of our servers for you the download. All data is typically retained for 60 days. We STRONGLY encourage you to keep a local copy of your data as soon as possible. Contact [mailto:bmc-data@mit.edu bmc-data@mit.edu] if you have any issues.&lt;br /&gt;
&lt;br /&gt;
=== MIT ===&lt;br /&gt;
{| class=&#039;wikitable&#039;&lt;br /&gt;
 |&#039;&#039;&#039;LURIA (MIT VPN required)&#039;&#039;&#039;||&lt;br /&gt;
* samples can be directly copied from our share /net/bmc-pub17/data/bmc/public/[LAB]/[PROJECT]&lt;br /&gt;
* Please ensure you are running cp from a COMPUTING NODE of Luria instead of the head node&lt;br /&gt;
 |-&lt;br /&gt;
 |&#039;&#039;&#039;Windows/Mac SFTP client (MIT VPN required)||&lt;br /&gt;
* Mac: Fetch | Windows: SecureCRT/FX. Download from [https://ist.mit.edu/software/filetransfer Secure File Transfer at MIT]&lt;br /&gt;
 |-&lt;br /&gt;
 |&#039;&#039;&#039;Web Download &amp;lt;p&amp;gt; (MIT Touchstone Login required)&#039;&#039;&#039;||&lt;br /&gt;
https://bmc-data.mit.edu/[LAB]/[PROJECT]&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
=== Globus ===&lt;br /&gt;
Globus is a file transfer service that makes it easy to transfer files between projects and institutions. You&#039;ll need to [https://www.globus.org/get-started register for a Globus account] to use Globus. The data ready e-mail will have a link to your project&#039;s files on Globus, but you can also navigate to them using one of these links:&lt;br /&gt;
&lt;br /&gt;
{| class=&#039;wikitable&#039;&lt;br /&gt;
  |&#039;&#039;&#039;Internal Labs&#039;&#039;&#039;||&lt;br /&gt;
[https://app.globus.org/file-manager/collections/3b0a124f-c273-4150-b383-99ed8c8a2764/overview Koch Institute BioMicro Data (Internal)]&lt;br /&gt;
  |-&lt;br /&gt;
  |&#039;&#039;&#039;External Labs&#039;&#039;&#039;||&lt;br /&gt;
[https://app.globus.org/file-manager/collections/31ca1b0a-1deb-4882-93f0-6e86fe9ebe3a/overview Koch Institute BioMicro Data (External)]&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
If your institution uses Globus, you can transfer your data from our collection to your institution&#039;s collection by following [https://docs.globus.org/guides/tutorials/manage-files/transfer-files/ these instructions.]&lt;br /&gt;
&lt;br /&gt;
Otherwise, if you&#039;d like to download your data to a personal workstation, you can download [https://www.globus.org/globus-connect-personal Globus Connect Personal]. After following the installation instructions, your computer will be available as a collection in Globus, and you can follow [https://docs.globus.org/guides/tutorials/manage-files/transfer-files/ these instructions] to download your data.&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;If you do not have permissions to view or download files, please e-mail [mailto:luria-help@mit.edu luria-help@mit.edu] with your Globus account e-mail, your lab association, and optionally a list of members in the lab who should be granted access to the data as well. Please make sure these members have Globus accounts before sending the e-mail. You can also e-mail [mailto:luria-help@mit.edu luria-help@mit.edu] to have us revoke people&#039;s access.&#039;&#039;&#039;&lt;br /&gt;
&lt;br /&gt;
=== Read archival ===&lt;br /&gt;
&lt;br /&gt;
Users are strongly encouraged to retain a local copy of their data as early as possible after sequencing result delivery. &lt;br /&gt;
&lt;br /&gt;
&amp;lt;b&amp;gt;After 60 days, fastq files will be compressed on the server. Bam files, containing reads aligned either to the genome mentioned in the sample submission form or phiX (a phage genome), will be deleted to save storage space.&amp;lt;/b&amp;gt;&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
&lt;br /&gt;
Older files: Prior to 2020, bam files were maintained as archival files. Fastq files can be regenerated from the bam files (samtools sort -n to sort bam files by read names, and converted back to fastq files using bedtools bamToFastq).&lt;br /&gt;
&lt;br /&gt;
== UPLOADING DATA ==&lt;br /&gt;
&lt;br /&gt;
To upload your data, you can access our file server named bmc-opendata.mit.edu via the sftp protocol. &lt;br /&gt;
&lt;br /&gt;
* Request SFTP account with projects and lab information for storage setup&lt;br /&gt;
* Use a SFTP client (such as WinSCP, FileZilla, PuTTY and Cyberduck for Windows/Mac or sftp command for Linux)&lt;br /&gt;
&lt;br /&gt;
&amp;lt;!---&lt;br /&gt;
== SUBMISSIONS ==&lt;br /&gt;
=== Volume/Concentration ===&lt;br /&gt;
The volume and concentration needed for submission will depend on the type of sample prep.  However, if you are submitting prepped, sequence-ready samples, we require a minimum of 10uL at a concentration of at least 2.0 nM.  This allows us to perform quality control and have enough of the library for clustering.  Any samples less than 2.0 nM cause significant difficulties with clustering the flowcell, which is why we cannot guarantee optimal read count and quality should you like us to proceed with sequencing a low concentration sample.&lt;br /&gt;
&lt;br /&gt;
=== Custom Primers ===&lt;br /&gt;
When submitting [[BioMicroCenter:Sequencing|&#039;&#039;&#039;custom primers&#039;&#039;&#039;]], be sure to verify their compatibility with the standard Illumina Primers used.  If you&#039;d like a second opinion on the compatibility of your primers, we&#039;d be more than happy to sit down with you and verify the primer design based on your constructs.&lt;br /&gt;
&lt;br /&gt;
Another important aspect to consider is the fact that clustering conditions differ when comparing the HiSeq and NextSeq to the MiSeq.  The MiSeq clusters at about 5 degrees higher than either the NextSeq or the cBot, which is used to prepare flowcells for the HiSeq.  This means that all custom primers must be designed appropriately so Tm&#039;s are compatible with whatever sequencing route you choose.&lt;br /&gt;
&lt;br /&gt;
Submit at least 20 microliters at 100 micromolar concentration of each of your primers per each intended lane of sequencing.&lt;br /&gt;
&lt;br /&gt;
== How long will it take for my HiSeq sample to be sequenced? ==&lt;br /&gt;
This is a very hard question to answer because it involves a number of variables. The simplest answer is, sample preparation typically takes ~1 weeks for standard samples with all reagents on hand (QC, prep, QC). For newer or rarely used methods, the number can extend to a month if we need to gather reagents. Once the sample is ready, it goes in to the sequencing queue. This queue is often the longest part of the process. Once the full flowcell is ready and put on a sequencer, it takes ~1.3h per base to sequence (this used to be 1h but upgrades to the machines have slowed them down some). A 40nt read takes just over 2 days. Turn around (for PE samples) functionally takes about a 1/2 day. So a 40+40 PE sample will take about 6 days once it is on the sequencer - if nothing goes wrong. Overall, when the queue is flowing at full speed, a 40nt SE submission with sample preparation can be returned with data in right around 3 weeks. 40+40 PE samples more typically take ~4-6 weeks for [[BioMicroCenter:CoreDeps|CORE lab members]] with the extra time coming from delays in filling out flowcells. &lt;br /&gt;
&lt;br /&gt;
NextSeq and MiSeq are much simpler. Once the sample is prepped and the machine is working, it will be loaded immediately. Total times should be less than 2 weeks.&lt;br /&gt;
&lt;br /&gt;
=== Illumina Queue: Full HiSeq Flowcells === &lt;br /&gt;
The primary requirement for a HiSeq run is a full flowcell. Each flowcell is composed of 7 lanes that must be run together. In the BioMicro Center, lanes are grouped by read length to optimize throughput and keep costs low. This requirement for 7 samples has a major impact on queue time. If you do a common read length, such as 40nt SE, the queue length will be short. On the other hand, if you ask for an unusual read length, your samples may never come off the queue. By frequency, the most common read lengths are, in order: &#039;&#039;&#039;40SE, 50SE, and 40+40PE&#039;&#039;&#039;. We do fudge a little in some of the long read lengths to fit samples in (mixing 40 and 50 for example), but we will always give priority to the samples of the read length we are going to do. We do not run incomplete flowcells and other options, such as NextSeq and MiSeq exist for unusual read lengths. NOTE: Barcoding is assumed to be done on all flowcells and we do NOT restrict pooling based on which samples are multiplexed and which are not.&lt;br /&gt;
&lt;br /&gt;
=== Illumina Queue: Priority ===&lt;br /&gt;
{|&lt;br /&gt;
 |width=450|&lt;br /&gt;
The BioMicro Center queue operates on a tiered priority basis (see table). We hold to this priority schema as closely as we can while still needing to fill flowcells. Some caveats: Once a flowcell is quality controlled and &amp;quot;on deck&amp;quot; for clustering, that flowcell is considered locked and no new submissions will be added to it. Clustering can happen a week or more before loading. Once flowcells are clustered, their order is set barring the need for reruns.&lt;br /&gt;
 |&lt;br /&gt;
{| align=&amp;quot;right&amp;quot;, textalign=&amp;quot;center&amp;quot;, border=1 &lt;br /&gt;
 ! Priority&lt;br /&gt;
 ! Type&lt;br /&gt;
 |-&lt;br /&gt;
 | 1&lt;br /&gt;
 | Reruns of failures&lt;br /&gt;
 |-&lt;br /&gt;
 | 2&lt;br /&gt;
 | Samples from labs that bought the equipment &lt;br /&gt;
 |-&lt;br /&gt;
 | 3&lt;br /&gt;
 | [[BioMicroCenter:CoreDeps|MIT CORE labs]]&lt;br /&gt;
 |-&lt;br /&gt;
 | 4&lt;br /&gt;
 | MIT non-CORE labs&lt;br /&gt;
 |-&lt;br /&gt;
 | 5&lt;br /&gt;
 | non-MIT users&lt;br /&gt;
|}&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
=== Failures ===&lt;br /&gt;
The Illumina HiSeq is temperamental beast and failures do happen. The large majority of the failures on our sequencer are due to equipment failure and not due to operator failure, although those do happen as well. Once a failed run is identified, we must work with Illumina to identify the cause and prove to them that the cause is equipment based. This allows us to get replacement reagents which helps keep your costs down. Once a run has failed, the failed samples on that flowcell are given highest priority. &lt;br /&gt;
&lt;br /&gt;
Failures create a large challenge for us in providing estimates of when your data will be ready. Because the failure rate is so high and failures tend to cluster, short sequencing estimates can quickly turn in to long waits, particularly if you are not at the top of the priority queue. The BioMicro Center works closely with the KI Biopolymer Core and the Whitehead Institute GTC to move samples to other machines whenever possible if we develop a backlog. &lt;br /&gt;
&lt;br /&gt;
=== Triage ===&lt;br /&gt;
The final consideration in timing is best summarized as &amp;quot;Triage&amp;quot;. This encompasses all of the many other factors we take in to account in creating flowcells. This can range from scheduling: Pushing a 40nt SE run on before an 40+40PE run so the turn around of the PE times to be during the week instead of over a weekend - to flowcell logistics: Grabbing low priority 40SE samples to fill out a rerun flowcell while an 40+40PE run that does not have rerun samples waits in the queue. Our goal is to get everyone their data as fast as we possibly can. We look at every project every day and we are working as hard as we can to get you your data as fast as we can.&lt;br /&gt;
--&amp;gt;&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:Submission&amp;diff=131238</id>
		<title>BioMicroCenter:Submission</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:Submission&amp;diff=131238"/>
		<updated>2026-03-27T01:09:13Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: Add iLabs capture confirmation and sample drop-off note for MIT users&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;{{BioMicroCenter}}&lt;br /&gt;
&lt;br /&gt;
== Submitting to the BioMicro Center ==&lt;br /&gt;
&lt;br /&gt;
The BioMicro Center accepts samples from MIT-affiliated and external users. For an overview of available services, see the [[BioMicroCenter|home page]].&lt;br /&gt;
&lt;br /&gt;
----&lt;br /&gt;
&lt;br /&gt;
== MIT Users ==&lt;br /&gt;
&lt;br /&gt;
MIT users access the BioMicro Center through [https://mit-ki.ilabsolutions.com/sc/3381/ki-genomics-core-mit-biomicro-center/?tab=about iLabs], which requires an MIT Kerberos account and an active MIT cost object. Kerberos accounts are available to members of MIT, the Whitehead Institute, and qualifying users at the Broad and Ragon Institutes. If you do not have a Kerberos account, see [https://ist.mit.edu/accounts MIT IS&amp;amp;T Accounts].&lt;br /&gt;
&lt;br /&gt;
See [[BioMicroCenter:Pricing|Pricing]] for current rates.&lt;br /&gt;
&lt;br /&gt;
=== Assisted services ===&lt;br /&gt;
&lt;br /&gt;
Assisted services are those where samples are delivered to the BioMicro Center and processed by staff. These include sequencing, library preparation, sample QC, and informatics.&lt;br /&gt;
&lt;br /&gt;
To submit, log in to iLabs and follow these paths:&lt;br /&gt;
&lt;br /&gt;
* [https://mit-ki.ilabsolutions.com/sc/3381/ki-genomics-core-mit-biomicro-center?tab=services &#039;&#039;&#039;Genomics Services&#039;&#039;&#039;] (sequencing, library preparation, sample QC): Request Services → BioMicro Requests → Initiate Request&lt;br /&gt;
* [https://mit-ki.ilabsolutions.com/sc/3189/bioinformatics-and-computing-core/?tab=services &#039;&#039;&#039;Informatics Services&#039;&#039;&#039;] (bioinformatics and computing): Request Services → Initiate Request&lt;br /&gt;
&lt;br /&gt;
After submitting, you will receive an email once your project has been captured from iLabs. Once you have received that confirmation, bring your samples to 68-322 during regular business hours. If you cannot access the building, call the lab at 617-715-4533.&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Purchase orders:&#039;&#039;&#039; If you are paying by PO, you must submit with an MIT cost object. Fields for the PO number are included in the submission forms. The MIT cost object is a backup that will only be charged if payment via PO cannot be collected.&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Approval:&#039;&#039;&#039; The BioMicro Center does not hold projects waiting for financial approval. If a submission is not rejected in a timely manner, we will proceed and recharge normally. Please ensure your cost object is active before submitting.&lt;br /&gt;
&lt;br /&gt;
Once your data is ready, see [[BioMicroCenter:FAQ#DOWNLOADING_DATA|Downloading your data]] for instructions on retrieving results.&lt;br /&gt;
&lt;br /&gt;
=== Walk-up services ===&lt;br /&gt;
&lt;br /&gt;
Walk-up services are those where users operate equipment themselves after receiving training. To schedule equipment, log in to [https://mit-ki.ilabsolutions.com/sc/3381/ki-genomics-core-mit-biomicro-center/?tab=about iLabs] and follow this path:&lt;br /&gt;
&lt;br /&gt;
Schedule Equipment → expand the service → View Schedule&lt;br /&gt;
&lt;br /&gt;
Some equipment requires training before sign-up is permitted. Contact [mailto:biomicro@mit.edu biomicro@mit.edu] to arrange training.&lt;br /&gt;
&lt;br /&gt;
After-hours access to the core is part of the walk-up process and requires a one-time form:&lt;br /&gt;
* [https://www.dropbox.com/scl/fi/nxdzbesvp2mab69wofotm/New_User_Sign_Up_Form.docx?rlkey=x9ftkatdlm897fbeol2ibv8tb&amp;amp;st=ag0jsvn9&amp;amp;dl=0 After-Hours Access Form]&lt;br /&gt;
&lt;br /&gt;
=== New labs ===&lt;br /&gt;
&lt;br /&gt;
New labs are set up in iLabs by your PI or a designated administrator — having MIT spending permission does not automatically carry over to iLabs. For assistance getting your lab registered, see [https://ki-sbc.mit.edu/core-access KI Shared Research Core Access] or contact [mailto:ciminojv@mit.edu Jack Cimino] at the Koch Institute.&lt;br /&gt;
&lt;br /&gt;
----&lt;br /&gt;
&lt;br /&gt;
== External Users ==&lt;br /&gt;
&lt;br /&gt;
The BioMicro Center primarily serves the MIT community. We welcome external users when capacity allows, with the understanding that MIT samples always have priority. &#039;&#039;&#039;The BioMicro Center reserves the right to decline any submission.&#039;&#039;&#039;&lt;br /&gt;
&lt;br /&gt;
See [[BioMicroCenter:Pricing|Pricing]] for current rates.&lt;br /&gt;
&lt;br /&gt;
=== New external labs ===&lt;br /&gt;
&lt;br /&gt;
Each new external lab must complete a one-time form before quotes can be provided:&lt;br /&gt;
* [https://www.dropbox.com/s/tgnmr6lrubprjzx/New_External%20User%20Form_2018.xlsx?dl=0 New External User Form] — required once per lab&lt;br /&gt;
&lt;br /&gt;
=== Assisted services ===&lt;br /&gt;
&lt;br /&gt;
Assisted services are those where samples are delivered to the BioMicro Center and processed by staff.&lt;br /&gt;
&lt;br /&gt;
To submit samples, download and complete the appropriate form below and send it along with your samples.&lt;br /&gt;
&lt;br /&gt;
If you are bringing samples in person, please email [mailto:biomicro@mit.edu biomicro@mit.edu] in advance to coordinate a drop-off date and time. Once that is arranged, you are welcome to come by 68-322 during regular business hours. If you cannot access the building on arrival, call the lab at 617-715-4533.&lt;br /&gt;
&lt;br /&gt;
If you are shipping samples, see the shipping instructions below.&lt;br /&gt;
&lt;br /&gt;
* [https://www.dropbox.com/scl/fi/7jfss8ofhp98g0doiv9ph/BMC_EXT_shortRead25.xlsx?rlkey=syhww29wpavjakekc2d5lyskl&amp;amp;dl=0 Short Read Library Prep and/or Sequencing] — multipage form; separate tabs for prep and sequencing&lt;br /&gt;
* [https://www.dropbox.com/scl/fi/lgnx0bbfz3lrn00zyplyw/LongReadSeq_User_Form.xlsx?dl=0&amp;amp;rlkey=x1tbksv8swv5xhvp91m68eg42 Long Read Sequencing]&lt;br /&gt;
* [https://www.dropbox.com/s/kuodtqu77079st7/BMC_EXT_FAFP_v4.xlsx?dl=0 Advanced Analytical / Bioanalyzer]&lt;br /&gt;
&lt;br /&gt;
Ship DNA samples at 4°C; ship RNA samples on dry ice. Use overnight delivery and address packages to:&lt;br /&gt;
&lt;br /&gt;
  MIT BioMicro Center&lt;br /&gt;
  31 Ames Street, Building 68-322&lt;br /&gt;
  Cambridge, MA 02139&lt;br /&gt;
&lt;br /&gt;
Please email an electronic copy of your completed order form and PO to [mailto:biomicro@mit.edu biomicro@mit.edu].&lt;br /&gt;
&lt;br /&gt;
Once your data is ready, see [[BioMicroCenter:FAQ#DOWNLOADING_DATA|Downloading your data]] for instructions on retrieving results.&lt;br /&gt;
&lt;br /&gt;
=== Walk-up services ===&lt;br /&gt;
&lt;br /&gt;
Walk-up services for external users are generally performed as assisted services, with staff operating the equipment at a nominal fee above the standard equipment rate. This allows scheduling around MIT user demand. After-hours access is not available to external users.&lt;br /&gt;
&lt;br /&gt;
To arrange walk-up access, contact [mailto:biomicro@mit.edu biomicro@mit.edu].&lt;br /&gt;
&lt;br /&gt;
=== Billing ===&lt;br /&gt;
&lt;br /&gt;
Please email purchase orders to [mailto:bio-billing@mit.edu bio-billing@mit.edu].&lt;br /&gt;
&lt;br /&gt;
Direct payment inquiries and checks to:&lt;br /&gt;
&lt;br /&gt;
  Massachusetts Institute of Technology&lt;br /&gt;
  Biology Finance Office&lt;br /&gt;
  77 Massachusetts Avenue, 68-157&lt;br /&gt;
  Cambridge, MA 02139&lt;br /&gt;
&lt;br /&gt;
For a W-9: [https://vpf.mit.edu/forms/7/1220 MIT W-9 Form]&lt;br /&gt;
&lt;br /&gt;
A Technical Services Agreement (TSA) — pre-approved by MIT legal and not open to redlining — is available upon request. Contact [mailto:biomicro@mit.edu biomicro@mit.edu] for details.&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:Submission&amp;diff=131237</id>
		<title>BioMicroCenter:Submission</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:Submission&amp;diff=131237"/>
		<updated>2026-03-27T01:08:53Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: Clarify sample drop-off instructions for MIT and external users&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;{{BioMicroCenter}}&lt;br /&gt;
&lt;br /&gt;
== Submitting to the BioMicro Center ==&lt;br /&gt;
&lt;br /&gt;
The BioMicro Center accepts samples from MIT-affiliated and external users. For an overview of available services, see the [[BioMicroCenter|home page]].&lt;br /&gt;
&lt;br /&gt;
----&lt;br /&gt;
&lt;br /&gt;
== MIT Users ==&lt;br /&gt;
&lt;br /&gt;
MIT users access the BioMicro Center through [https://mit-ki.ilabsolutions.com/sc/3381/ki-genomics-core-mit-biomicro-center/?tab=about iLabs], which requires an MIT Kerberos account and an active MIT cost object. Kerberos accounts are available to members of MIT, the Whitehead Institute, and qualifying users at the Broad and Ragon Institutes. If you do not have a Kerberos account, see [https://ist.mit.edu/accounts MIT IS&amp;amp;T Accounts].&lt;br /&gt;
&lt;br /&gt;
See [[BioMicroCenter:Pricing|Pricing]] for current rates.&lt;br /&gt;
&lt;br /&gt;
=== Assisted services ===&lt;br /&gt;
&lt;br /&gt;
Assisted services are those where samples are delivered to the BioMicro Center and processed by staff. These include sequencing, library preparation, sample QC, and informatics.&lt;br /&gt;
&lt;br /&gt;
To submit, log in to iLabs and follow these paths:&lt;br /&gt;
&lt;br /&gt;
* [https://mit-ki.ilabsolutions.com/sc/3381/ki-genomics-core-mit-biomicro-center?tab=services &#039;&#039;&#039;Genomics Services&#039;&#039;&#039;] (sequencing, library preparation, sample QC): Request Services → BioMicro Requests → Initiate Request&lt;br /&gt;
* [https://mit-ki.ilabsolutions.com/sc/3189/bioinformatics-and-computing-core/?tab=services &#039;&#039;&#039;Informatics Services&#039;&#039;&#039;] (bioinformatics and computing): Request Services → Initiate Request&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Purchase orders:&#039;&#039;&#039; If you are paying by PO, you must submit with an MIT cost object. Fields for the PO number are included in the submission forms. The MIT cost object is a backup that will only be charged if payment via PO cannot be collected.&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Approval:&#039;&#039;&#039; The BioMicro Center does not hold projects waiting for financial approval. If a submission is not rejected in a timely manner, we will proceed and recharge normally. Please ensure your cost object is active before submitting.&lt;br /&gt;
&lt;br /&gt;
Once your data is ready, see [[BioMicroCenter:FAQ#DOWNLOADING_DATA|Downloading your data]] for instructions on retrieving results.&lt;br /&gt;
&lt;br /&gt;
=== Walk-up services ===&lt;br /&gt;
&lt;br /&gt;
Walk-up services are those where users operate equipment themselves after receiving training. To schedule equipment, log in to [https://mit-ki.ilabsolutions.com/sc/3381/ki-genomics-core-mit-biomicro-center/?tab=about iLabs] and follow this path:&lt;br /&gt;
&lt;br /&gt;
Schedule Equipment → expand the service → View Schedule&lt;br /&gt;
&lt;br /&gt;
Some equipment requires training before sign-up is permitted. Contact [mailto:biomicro@mit.edu biomicro@mit.edu] to arrange training.&lt;br /&gt;
&lt;br /&gt;
After-hours access to the core is part of the walk-up process and requires a one-time form:&lt;br /&gt;
* [https://www.dropbox.com/scl/fi/nxdzbesvp2mab69wofotm/New_User_Sign_Up_Form.docx?rlkey=x9ftkatdlm897fbeol2ibv8tb&amp;amp;st=ag0jsvn9&amp;amp;dl=0 After-Hours Access Form]&lt;br /&gt;
&lt;br /&gt;
=== New labs ===&lt;br /&gt;
&lt;br /&gt;
New labs are set up in iLabs by your PI or a designated administrator — having MIT spending permission does not automatically carry over to iLabs. For assistance getting your lab registered, see [https://ki-sbc.mit.edu/core-access KI Shared Research Core Access] or contact [mailto:ciminojv@mit.edu Jack Cimino] at the Koch Institute.&lt;br /&gt;
&lt;br /&gt;
----&lt;br /&gt;
&lt;br /&gt;
== External Users ==&lt;br /&gt;
&lt;br /&gt;
The BioMicro Center primarily serves the MIT community. We welcome external users when capacity allows, with the understanding that MIT samples always have priority. &#039;&#039;&#039;The BioMicro Center reserves the right to decline any submission.&#039;&#039;&#039;&lt;br /&gt;
&lt;br /&gt;
See [[BioMicroCenter:Pricing|Pricing]] for current rates.&lt;br /&gt;
&lt;br /&gt;
=== New external labs ===&lt;br /&gt;
&lt;br /&gt;
Each new external lab must complete a one-time form before quotes can be provided:&lt;br /&gt;
* [https://www.dropbox.com/s/tgnmr6lrubprjzx/New_External%20User%20Form_2018.xlsx?dl=0 New External User Form] — required once per lab&lt;br /&gt;
&lt;br /&gt;
=== Assisted services ===&lt;br /&gt;
&lt;br /&gt;
Assisted services are those where samples are delivered to the BioMicro Center and processed by staff.&lt;br /&gt;
&lt;br /&gt;
To submit samples, download and complete the appropriate form below and send it along with your samples.&lt;br /&gt;
&lt;br /&gt;
If you are bringing samples in person, please email [mailto:biomicro@mit.edu biomicro@mit.edu] in advance to coordinate a drop-off date and time. Once that is arranged, you are welcome to come by 68-322 during regular business hours. If you cannot access the building on arrival, call the lab at 617-715-4533.&lt;br /&gt;
&lt;br /&gt;
If you are shipping samples, see the shipping instructions below.&lt;br /&gt;
&lt;br /&gt;
* [https://www.dropbox.com/scl/fi/7jfss8ofhp98g0doiv9ph/BMC_EXT_shortRead25.xlsx?rlkey=syhww29wpavjakekc2d5lyskl&amp;amp;dl=0 Short Read Library Prep and/or Sequencing] — multipage form; separate tabs for prep and sequencing&lt;br /&gt;
* [https://www.dropbox.com/scl/fi/lgnx0bbfz3lrn00zyplyw/LongReadSeq_User_Form.xlsx?dl=0&amp;amp;rlkey=x1tbksv8swv5xhvp91m68eg42 Long Read Sequencing]&lt;br /&gt;
* [https://www.dropbox.com/s/kuodtqu77079st7/BMC_EXT_FAFP_v4.xlsx?dl=0 Advanced Analytical / Bioanalyzer]&lt;br /&gt;
&lt;br /&gt;
Ship DNA samples at 4°C; ship RNA samples on dry ice. Use overnight delivery and address packages to:&lt;br /&gt;
&lt;br /&gt;
  MIT BioMicro Center&lt;br /&gt;
  31 Ames Street, Building 68-322&lt;br /&gt;
  Cambridge, MA 02139&lt;br /&gt;
&lt;br /&gt;
Please email an electronic copy of your completed order form and PO to [mailto:biomicro@mit.edu biomicro@mit.edu].&lt;br /&gt;
&lt;br /&gt;
Once your data is ready, see [[BioMicroCenter:FAQ#DOWNLOADING_DATA|Downloading your data]] for instructions on retrieving results.&lt;br /&gt;
&lt;br /&gt;
=== Walk-up services ===&lt;br /&gt;
&lt;br /&gt;
Walk-up services for external users are generally performed as assisted services, with staff operating the equipment at a nominal fee above the standard equipment rate. This allows scheduling around MIT user demand. After-hours access is not available to external users.&lt;br /&gt;
&lt;br /&gt;
To arrange walk-up access, contact [mailto:biomicro@mit.edu biomicro@mit.edu].&lt;br /&gt;
&lt;br /&gt;
=== Billing ===&lt;br /&gt;
&lt;br /&gt;
Please email purchase orders to [mailto:bio-billing@mit.edu bio-billing@mit.edu].&lt;br /&gt;
&lt;br /&gt;
Direct payment inquiries and checks to:&lt;br /&gt;
&lt;br /&gt;
  Massachusetts Institute of Technology&lt;br /&gt;
  Biology Finance Office&lt;br /&gt;
  77 Massachusetts Avenue, 68-157&lt;br /&gt;
  Cambridge, MA 02139&lt;br /&gt;
&lt;br /&gt;
For a W-9: [https://vpf.mit.edu/forms/7/1220 MIT W-9 Form]&lt;br /&gt;
&lt;br /&gt;
A Technical Services Agreement (TSA) — pre-approved by MIT legal and not open to redlining — is available upon request. Contact [mailto:biomicro@mit.edu biomicro@mit.edu] for details.&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:News&amp;diff=131236</id>
		<title>BioMicroCenter:News</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:News&amp;diff=131236"/>
		<updated>2026-03-27T00:47:36Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: Remove About block (moved to BioMicroCenter:AboutBMC); update website URL to bmcwiki.mit.edu; rename NIH section heading&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;{{BioMicroCenter}}&lt;br /&gt;
&lt;br /&gt;
.&lt;br /&gt;
== Welcome to the MIT BIOMICRO CENTER ==&lt;br /&gt;
&lt;br /&gt;
{|&lt;br /&gt;
|valign=top style=&amp;quot;width:60%;padding-right:10px;&amp;quot;| &lt;br /&gt;
== BioMicro Center News ==&lt;br /&gt;
&lt;br /&gt;
=== SUMMER 2025 ===&lt;br /&gt;
&amp;lt;B&amp;gt;Sequencing Updates:&amp;lt;/B&amp;gt;&lt;br /&gt;
We are completing an overhaul of the sequencing technologies in the core and will be retiring many of our older sequencers in the very near future. The new offerings we have introduced over the last few years provide significant improvements in quality and price point and support both larger and smaller scale experiments. &lt;br /&gt;
&lt;br /&gt;
&amp;lt;U&amp;gt;High Yield Sequencers (&amp;gt;1b reads)&amp;lt;/U&amp;gt;: The price war on the high-end sequencers has made the NovaSeq6000 in the core all but obsolete, but has also created a glut of capacity in the area. To access these lower prices we have transitioned to an outsourcing model using the NovaSeqX, Illumina&#039;s top end sequencer, taking advantage of placements at neighboring academic institutions (DFCI, Broad, Harvard). We have worked closely with these shared resources to provide the best prices we can on each service. All the logistics (from QC to data delivery) will still be handled by the BioMicro Center and it will work just as if you are submitting to the core. As we do have preferential pricing at many of these institutions, we cannot reasonably place the pricing on our website. Instead, you will be able to see the pricing as you submit on ilabs (or by request). &lt;br /&gt;
&lt;br /&gt;
&amp;lt;U&amp;gt;Mid Yield Sequencers (250m-1.2b)&amp;lt;/U&amp;gt;: BMC will continue to support mid-yield sequencing on site with our Element AVITI24. The AVITI provides both a higher quality read than Illumina as well as a dramatically better price than the older NextSeq500. We anticipate a software upgrade in the next few weeks that will also increase the yield per lane from 400m to ~600m reads.&lt;br /&gt;
&lt;br /&gt;
&amp;lt;U&amp;gt;Low Yield Sequencers (&amp;lt;250m reads)&amp;lt;/U&amp;gt;: BMC will also support low yield experiments with both the Singular G4 as well as a new MiSeq i100. The Singular G4 is the lowest cost sequencer we now have with 100m reads available for $300 (50PE). It does require different anchor sequences, but is perfect for pilot experiments. For those needing longer reads or P5/P7 anchors, we are also acquiring a MiSeq i100 to replace the aging MiSeqs in the core. Importantly, the i100 will be available both as an assisted service and as a true walkup sequencer, available to anyone with training. Each run of the i100 is significantly faster than the older MiSeq (600nt runs take only 1 day) with some significant price improvements as well. A huge thank you to the VPR equipment replacement fund, Department of Biology, Gene Li, Michael Birnbaum, and an alumni donor for making the MiSeq i100 possible.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&amp;lt;B&amp;gt;UPCOMING TECHNOLOGY SEMINARS:&amp;lt;/B&amp;gt;&lt;br /&gt;
* Friday, July 25th Covaris: Covaris R230 in extraction, chromatin assays, protein and NGS library prep.&lt;br /&gt;
* Tuesday, Aug 19th  10X Genomics: Fresh Frozen/polyA based chemistry added to the Visium HD&lt;br /&gt;
* TBD, Sept  Illumina: Introduction to the MiSeq i100&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
=== SPRING 2025 ===&lt;br /&gt;
&lt;br /&gt;
&amp;lt;B&amp;gt;Spatial Transcriptomics:&amp;lt;/B&amp;gt;&lt;br /&gt;
- The BioMicro Center has recently expanded our spatial &#039;omics options with the addition of the AVITI24. This upgrade to our existing Element sequencer enables it to do in situ sequencing. For this method, cells are either grown directly onto a flowcell or placed there, fixed and panels of transcripts identified. The system can currently handle up to 350 transcripts and dozens of antibodies with cellular features identified as well (nuclei, cytoskeletin, membrane, etc.). More information is on the Element website. The upgrade was a generous gift from Scott Ritterbush.&lt;br /&gt;
&lt;br /&gt;
TO highlight the new capabilities, we have a special seminar upcoming. Molly He, CEO of Element, will be speaking at the KI on May8 (12n). She&#039;ll be talking both about the science of the AVITI and the AVITI24, but also about building a company that is competing with the near monopolistic power of Illumina. I hope you can make it to what should be a fantastic seminar.&lt;br /&gt;
&lt;br /&gt;
&amp;lt;B&amp;gt;High Output Sequencing:&amp;lt;/B&amp;gt;&lt;br /&gt;
- The BioMicro Center is now supporting sequencing on Illumina&#039;s NovaSeqX through collaborations with our sister shared resources in the Boston area.  Practically, this will still act like any other sequencing: You will still submit on ilabs and bring the samples to the core. We will preform all the QC and receive the data back, process it, confrim the quality of the data (resolving any technical issues) and place it in your data delivery folders. Since the rates we can share with you are below the published rates, we cannot put these rates on our website, but they are visible in ilabs.&lt;br /&gt;
&lt;br /&gt;
&amp;lt;B&amp;gt;Website:&amp;lt;/B&amp;gt;&lt;br /&gt;
- Lastly, the website we have used for well over a decade appears to have failed. Openwetware, a scientific wiki platform, was built at MIT many years ago, starting in Bldg68, and the wiki seemed like a natural fit for the core and worked well for many year. The website failed on us a few weeks ago. We have set up a new page (still reachable at [http://bmcwiki.mit.edu bmcwiki.mit.edu]) but our backup was a few months old. We&#039;re working to get it back up to date and apologize for any missing information. If you do have questions or see broken links, please do let us know at biomico@mit.edu&lt;br /&gt;
|valign=&amp;quot;top&amp;quot;|&lt;br /&gt;
&lt;br /&gt;
== NIH ACKNOWLEDGMENT ==&lt;br /&gt;
&lt;br /&gt;
Experimental and analytical work done in the BioMicro Center is funded by the NIH and must be made available through the NIH&#039;s open access policy. All Koch Institute and CEHS labs &#039;&#039;&#039;must&#039;&#039;&#039; acknowledge their core grants for work done in the core with the following language. &lt;br /&gt;
* KI &#039;&#039;\&amp;quot;This work was funded by the National Cancer Institute of the NIH under award P30-CA14051\&amp;quot;&#039;&#039; &lt;br /&gt;
* [[BioMicroCenter:CEHS13|CEHS]] &#039;&#039;\&amp;quot;This work was funded by the National Institute of Environmental Health Sciences of the NIH under award P30-ES002109\&amp;quot;&#039;&#039;&lt;br /&gt;
&lt;br /&gt;
== PUBLICATIONS ==&lt;br /&gt;
&lt;br /&gt;
Publications from [https://www.ncbi.nlm.nih.gov/sites/myncbi/stuart.levine.1/bibliography/47165401/public/?sortby=pubDate&amp;amp;sdirection=descending Stuart Levine]&lt;br /&gt;
&lt;br /&gt;
== PREVIOUS NEWSLETTERS ==&lt;br /&gt;
&#039;&#039;&#039;[[BioMicroCenter:News2017+|2017+]]&#039;&#039;&#039;&amp;lt;BR&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;[[BioMicroCenter:News2016|2016]]&#039;&#039;&#039;&amp;lt;BR&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;[[BioMicroCenter:News2015|2015]]&#039;&#039;&#039;&amp;lt;BR&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;[[BioMicroCenter:News2014|2014]]&#039;&#039;&#039;&amp;lt;BR&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;[[BioMicroCenter:News2013|2013]]&#039;&#039;&#039;&amp;lt;BR&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;[[BioMicroCenter:News2012|2012]]&#039;&#039;&#039;&amp;lt;BR&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;[[BioMicroCenter:News2011|2011]]&#039;&#039;&#039;&amp;lt;BR&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;[[BioMicroCenter:News2010|2010]]&#039;&#039;&#039;&lt;br /&gt;
&amp;lt;br&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== RECENT CHANGES TO THE WEBSITE ==&lt;br /&gt;
{{BioMicroChanges}}&lt;br /&gt;
&lt;br /&gt;
|}&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:AboutBMC&amp;diff=131234</id>
		<title>BioMicroCenter:AboutBMC</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:AboutBMC&amp;diff=131234"/>
		<updated>2026-03-27T00:32:04Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: Create AboutBMC page: mission, services, staff, collaboration highlights, affiliations, NIH acknowledgment&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;{{BioMicroCenter}}&lt;br /&gt;
&lt;br /&gt;
== About the MIT BioMicro Center ==&lt;br /&gt;
&lt;br /&gt;
The MIT BioMicro Center is a genomics and bioinformatics core at MIT,&lt;br /&gt;
built around a simple idea: that the best science happens when expert&lt;br /&gt;
staff and cutting-edge technology work together with researchers as true&lt;br /&gt;
collaborators. Since 2000, we have supported work across the full breadth&lt;br /&gt;
of modern genomics, spanning sample preparation, single-cell and spatial&lt;br /&gt;
transcriptomics, library preparation, short- and long-read sequencing,&lt;br /&gt;
epigenomics, and bioinformatics, for labs across Biology, Biological&lt;br /&gt;
Engineering, the Koch Institute, and beyond.&lt;br /&gt;
&lt;br /&gt;
[[File:Bmc_campus_map.jpg|thumb|right|200px|BioMicro Center locations: Building 68 (main lab) and Building 76 (informatics).]]&lt;br /&gt;
&lt;br /&gt;
=== What We Do ===&lt;br /&gt;
&lt;br /&gt;
The Center offers end-to-end support across the major domains of&lt;br /&gt;
contemporary genomics:&lt;br /&gt;
&lt;br /&gt;
* &#039;&#039;&#039;[[BioMicroCenter:OtherTechnologies|Sample Preparation &amp;amp; QC]]&#039;&#039;&#039; — Nucleic acid extraction, fragment analysis, qPCR quantification, and oligonucleotide synthesis&lt;br /&gt;
* &#039;&#039;&#039;[[BioMicroCenter:SingleCell|Single-Cell &amp;amp; Spatial Genomics]]&#039;&#039;&#039; — 10X Chromium (3′ RNA, 5′ RNA, ATAC, Multiome), 10X Visium HD, and Element AVITI24 &amp;lt;i&amp;gt;in situ&amp;lt;/i&amp;gt; sequencing&lt;br /&gt;
* &#039;&#039;&#039;[[BioMicroCenter:Illumina_Library_Preparation|Library Preparation]]&#039;&#039;&#039; — Standard and high-throughput methods for DNA and RNA, including automated miniaturized protocols that reduce reagent costs by up to six-fold&lt;br /&gt;
* &#039;&#039;&#039;[[BioMicroCenter:Sequencing|Sequencing]]&#039;&#039;&#039; — High-, mid-, and low-yield short-read platforms (NovaSeqX*, AVITI24, Singular G4, MiSeq i100) plus long-read (ONT PromethION, PacBio Revio*)&lt;br /&gt;
&lt;br /&gt;
&amp;lt;small&amp;gt;* through collaboration with other shared resources&amp;lt;/small&amp;gt;&lt;br /&gt;
* &#039;&#039;&#039;[[BioMicroCenter:Computing|Data Management &amp;amp; Bioinformatics]]&#039;&#039;&#039; — Dedicated data storage and delivery infrastructure, a 58-node Linux cluster, analysis pipelines for standard and custom genomics workflows, and consulting from PhD-level informatics scientists. Data is allocated by lab and accessible across platforms, with backend connections to sequencing instruments for direct delivery.&lt;br /&gt;
&lt;br /&gt;
Both &#039;&#039;&#039;assisted services&#039;&#039;&#039; and &#039;&#039;&#039;walkup&#039;&#039;&#039; access are available depending on the application. All scheduling and recharge is handled through [https://mit.ilabsolutions.com iLabs].&lt;br /&gt;
&lt;br /&gt;
=== Our Staff ===&lt;br /&gt;
&lt;br /&gt;
The BioMicro Center is staffed by approximately 12 full-time research&lt;br /&gt;
scientists and technicians. The wet-lab team includes specialists in&lt;br /&gt;
library preparation, sequencing, automation, single-cell and spatial&lt;br /&gt;
methods, and quality control, with several staff holding advanced degrees&lt;br /&gt;
and research backgrounds in genomics. The informatics team includes&lt;br /&gt;
PhD-level bioinformatics scientists with expertise spanning RNAseq,&lt;br /&gt;
epigenomics, single-cell analysis, and long-read data, as well as&lt;br /&gt;
research computing specialists who work closely with labs on data&lt;br /&gt;
management — from experimental design and data organization through&lt;br /&gt;
deposition, sharing, and compliance with NIH open access requirements.&lt;br /&gt;
The Center administers dedicated storage and delivery infrastructure&lt;br /&gt;
and takes an active role in helping researchers meet FAIR data principles&lt;br /&gt;
and funder mandates, not just in providing the data itself.&lt;br /&gt;
All staff are cross-trained across methodologies, available for&lt;br /&gt;
consultation at any stage of a project, and regularly contribute to&lt;br /&gt;
methods development and peer-reviewed publications alongside MIT&lt;br /&gt;
researchers. The Center conducts multiple hands-on training sessions each&lt;br /&gt;
week to support independent instrument use.&lt;br /&gt;
&lt;br /&gt;
The Center is directed by &#039;&#039;&#039;[[User:Stuart S. Levine|Dr. Stuart Levine]]&#039;&#039;&#039;.&lt;br /&gt;
See the [[BioMicroCenter:People|full staff directory]].&lt;br /&gt;
&lt;br /&gt;
=== Recent Collaboration Highlights ===&lt;br /&gt;
&lt;br /&gt;
* &#039;&#039;&#039;Yilmaz / Jacks laboratories&#039;&#039;&#039; — RNAseq, CUT&amp;amp;RUN, ATACseq, and single-cell analysis identifying SOX17 as a key driver of immune evasion in colorectal cancer. &#039;&#039;Nature&#039;&#039; ([https://pubmed.ncbi.nlm.nih.gov/38418875/ PMID: 38418875])&lt;br /&gt;
* &#039;&#039;&#039;Sanchez-Rivera laboratory&#039;&#039;&#039; — High-throughput pegRNA library screening for p53 variants using Illumina and Singular G4 sequencing. &#039;&#039;Nature Biotechnology&#039;&#039; ([https://pubmed.ncbi.nlm.nih.gov/38472508/ PMID: 38472508])&lt;br /&gt;
* &#039;&#039;&#039;Henry / Griffith laboratories&#039;&#039;&#039; — Visium HD spatial transcriptomics to study ferroptosis in the uterine lining. Manuscript in preparation.&lt;br /&gt;
* &#039;&#039;&#039;White / Cima laboratories&#039;&#039;&#039; — Multi-omic extraction from single GBM biopsies for the BreakThrough Cancer Team Lab. &#039;&#039;Nature Communications&#039;&#039; ([https://pubmed.ncbi.nlm.nih.gov/40295505/ PMID: 40295505])&lt;br /&gt;
&lt;br /&gt;
== Affiliations &amp;amp; Governance ==&lt;br /&gt;
&lt;br /&gt;
The BioMicro Center is a joint endeavor of the&lt;br /&gt;
[http://biology.mit.edu Department of Biology],&lt;br /&gt;
the [http://web.mit.edu/be Department of Biological Engineering],&lt;br /&gt;
the [http://ki.mit.edu Koch Institute for Integrative Cancer Research]&lt;br /&gt;
(Integrated Genomics and Bioinformatics Core, IGB), and the&lt;br /&gt;
[http://cehs.mit.edu MIT Center for Environmental Health Sciences] (CEHS).&lt;br /&gt;
Operational direction is set by an &#039;&#039;&#039;Executive Committee&#039;&#039;&#039; composed of&lt;br /&gt;
the heads of each supporting unit, currently Amy Keating (Biology),&lt;br /&gt;
Peter Dedon (Biological Engineering), and Stefani Spranger (Koch Institute).&lt;br /&gt;
The Center is also guided by a &#039;&#039;&#039;Scientific Advisory Committee&#039;&#039;&#039; of nine&lt;br /&gt;
MIT faculty, three from each sponsoring department, which meets twice&lt;br /&gt;
yearly to help set technical priorities and ensure the Center remains&lt;br /&gt;
responsive to the needs of the research community.&lt;br /&gt;
&lt;br /&gt;
== Contact ==&lt;br /&gt;
&lt;br /&gt;
[http://whereis.mit.edu Building 68], Room 322 (main lab)&amp;lt;br&amp;gt;&lt;br /&gt;
Building 76 (informatics &amp;amp; research computing)&amp;lt;br&amp;gt;&lt;br /&gt;
biomicro@mit.edu&amp;lt;br&amp;gt;&lt;br /&gt;
617-715-4533&lt;br /&gt;
&lt;br /&gt;
== NIH Acknowledgment Requirements ==&lt;br /&gt;
&lt;br /&gt;
Researchers from the Koch Institute &#039;&#039;&#039;must&#039;&#039;&#039; include the&lt;br /&gt;
following acknowledgment language in any publication or presentation&lt;br /&gt;
based on work performed in the core:&lt;br /&gt;
&lt;br /&gt;
* &#039;&#039;&#039;KI labs:&#039;&#039;&#039; &#039;&#039;This work was funded by the National Cancer Institute of the NIH under award P30-CA14051.&#039;&#039;&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=File:Bmc_campus_map.jpg&amp;diff=131233</id>
		<title>File:Bmc campus map.jpg</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=File:Bmc_campus_map.jpg&amp;diff=131233"/>
		<updated>2026-03-27T00:31:47Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: Campus map showing BioMicro Center locations in Buildings 68 and 76&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;Campus map showing BioMicro Center locations in Buildings 68 and 76.&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:Submission&amp;diff=131232</id>
		<title>BioMicroCenter:Submission</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:Submission&amp;diff=131232"/>
		<updated>2026-03-26T02:19:39Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: Add Jack Cimino email, iLabs walk-up link, data download links for MIT and external users&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;{{BioMicroCenter}}&lt;br /&gt;
&lt;br /&gt;
== Submitting to the BioMicro Center ==&lt;br /&gt;
&lt;br /&gt;
The BioMicro Center accepts samples from MIT-affiliated and external users. For an overview of available services, see the [[BioMicroCenter|home page]].&lt;br /&gt;
&lt;br /&gt;
----&lt;br /&gt;
&lt;br /&gt;
== MIT Users ==&lt;br /&gt;
&lt;br /&gt;
MIT users access the BioMicro Center through [https://mit-ki.ilabsolutions.com/sc/3381/ki-genomics-core-mit-biomicro-center/?tab=about iLabs], which requires an MIT Kerberos account and an active MIT cost object. Kerberos accounts are available to members of MIT, the Whitehead Institute, and qualifying users at the Broad and Ragon Institutes. If you do not have a Kerberos account, see [https://ist.mit.edu/accounts MIT IS&amp;amp;T Accounts].&lt;br /&gt;
&lt;br /&gt;
See [[BioMicroCenter:Pricing|Pricing]] for current rates.&lt;br /&gt;
&lt;br /&gt;
=== Assisted services ===&lt;br /&gt;
&lt;br /&gt;
Assisted services are those where samples are delivered to the BioMicro Center and processed by staff. These include sequencing, library preparation, sample QC, and informatics.&lt;br /&gt;
&lt;br /&gt;
To submit, log in to iLabs and follow these paths:&lt;br /&gt;
&lt;br /&gt;
* [https://mit-ki.ilabsolutions.com/sc/3381/ki-genomics-core-mit-biomicro-center?tab=services &#039;&#039;&#039;Genomics Services&#039;&#039;&#039;] (sequencing, library preparation, sample QC): Request Services → BioMicro Requests → Initiate Request&lt;br /&gt;
* [https://mit-ki.ilabsolutions.com/sc/3189/bioinformatics-and-computing-core/?tab=services &#039;&#039;&#039;Informatics Services&#039;&#039;&#039;] (bioinformatics and computing): Request Services → Initiate Request&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Purchase orders:&#039;&#039;&#039; If you are paying by PO, you must submit with an MIT cost object. Fields for the PO number are included in the submission forms. The MIT cost object is a backup that will only be charged if payment via PO cannot be collected.&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Approval:&#039;&#039;&#039; The BioMicro Center does not hold projects waiting for financial approval. If a submission is not rejected in a timely manner, we will proceed and recharge normally. Please ensure your cost object is active before submitting.&lt;br /&gt;
&lt;br /&gt;
Once your data is ready, see [[BioMicroCenter:FAQ#DOWNLOADING_DATA|Downloading your data]] for instructions on retrieving results.&lt;br /&gt;
&lt;br /&gt;
=== Walk-up services ===&lt;br /&gt;
&lt;br /&gt;
Walk-up services are those where users operate equipment themselves after receiving training. To schedule equipment, log in to [https://mit-ki.ilabsolutions.com/sc/3381/ki-genomics-core-mit-biomicro-center/?tab=about iLabs] and follow this path:&lt;br /&gt;
&lt;br /&gt;
Schedule Equipment → expand the service → View Schedule&lt;br /&gt;
&lt;br /&gt;
Some equipment requires training before sign-up is permitted. Contact [mailto:biomicro@mit.edu biomicro@mit.edu] to arrange training.&lt;br /&gt;
&lt;br /&gt;
After-hours access to the core is part of the walk-up process and requires a one-time form:&lt;br /&gt;
* [https://www.dropbox.com/scl/fi/nxdzbesvp2mab69wofotm/New_User_Sign_Up_Form.docx?rlkey=x9ftkatdlm897fbeol2ibv8tb&amp;amp;st=ag0jsvn9&amp;amp;dl=0 After-Hours Access Form]&lt;br /&gt;
&lt;br /&gt;
=== New labs ===&lt;br /&gt;
&lt;br /&gt;
New labs are set up in iLabs by your PI or a designated administrator — having MIT spending permission does not automatically carry over to iLabs. For assistance getting your lab registered, see [https://ki-sbc.mit.edu/core-access KI Shared Research Core Access] or contact [mailto:ciminojv@mit.edu Jack Cimino] at the Koch Institute.&lt;br /&gt;
&lt;br /&gt;
----&lt;br /&gt;
&lt;br /&gt;
== External Users ==&lt;br /&gt;
&lt;br /&gt;
The BioMicro Center primarily serves the MIT community. We welcome external users when capacity allows, with the understanding that MIT samples always have priority. &#039;&#039;&#039;The BioMicro Center reserves the right to decline any submission.&#039;&#039;&#039;&lt;br /&gt;
&lt;br /&gt;
See [[BioMicroCenter:Pricing|Pricing]] for current rates.&lt;br /&gt;
&lt;br /&gt;
=== New external labs ===&lt;br /&gt;
&lt;br /&gt;
Each new external lab must complete a one-time form before quotes can be provided:&lt;br /&gt;
* [https://www.dropbox.com/s/tgnmr6lrubprjzx/New_External%20User%20Form_2018.xlsx?dl=0 New External User Form] — required once per lab&lt;br /&gt;
&lt;br /&gt;
=== Assisted services ===&lt;br /&gt;
&lt;br /&gt;
Assisted services are those where samples are delivered to the BioMicro Center and processed by staff.&lt;br /&gt;
&lt;br /&gt;
To submit samples, download the appropriate form below, fill it out, and send it along with your samples. Please email [mailto:biomicro@mit.edu biomicro@mit.edu] in advance to arrange a drop-off date and time.&lt;br /&gt;
&lt;br /&gt;
* [https://www.dropbox.com/scl/fi/7jfss8ofhp98g0doiv9ph/BMC_EXT_shortRead25.xlsx?rlkey=syhww29wpavjakekc2d5lyskl&amp;amp;dl=0 Short Read Library Prep and/or Sequencing] — multipage form; separate tabs for prep and sequencing&lt;br /&gt;
* [https://www.dropbox.com/scl/fi/lgnx0bbfz3lrn00zyplyw/LongReadSeq_User_Form.xlsx?dl=0&amp;amp;rlkey=x1tbksv8swv5xhvp91m68eg42 Long Read Sequencing]&lt;br /&gt;
* [https://www.dropbox.com/s/kuodtqu77079st7/BMC_EXT_FAFP_v4.xlsx?dl=0 Advanced Analytical / Bioanalyzer]&lt;br /&gt;
&lt;br /&gt;
Ship DNA samples at 4°C; ship RNA samples on dry ice. Use overnight delivery and address packages to:&lt;br /&gt;
&lt;br /&gt;
  MIT BioMicro Center&lt;br /&gt;
  31 Ames Street, Building 68-322&lt;br /&gt;
  Cambridge, MA 02139&lt;br /&gt;
&lt;br /&gt;
Please email an electronic copy of your completed order form and PO to [mailto:biomicro@mit.edu biomicro@mit.edu].&lt;br /&gt;
&lt;br /&gt;
Once your data is ready, see [[BioMicroCenter:FAQ#DOWNLOADING_DATA|Downloading your data]] for instructions on retrieving results.&lt;br /&gt;
&lt;br /&gt;
=== Walk-up services ===&lt;br /&gt;
&lt;br /&gt;
Walk-up services for external users are generally performed as assisted services, with staff operating the equipment at a nominal fee above the standard equipment rate. This allows scheduling around MIT user demand. After-hours access is not available to external users.&lt;br /&gt;
&lt;br /&gt;
To arrange walk-up access, contact [mailto:biomicro@mit.edu biomicro@mit.edu].&lt;br /&gt;
&lt;br /&gt;
=== Billing ===&lt;br /&gt;
&lt;br /&gt;
Please email purchase orders to [mailto:bio-billing@mit.edu bio-billing@mit.edu].&lt;br /&gt;
&lt;br /&gt;
Direct payment inquiries and checks to:&lt;br /&gt;
&lt;br /&gt;
  Massachusetts Institute of Technology&lt;br /&gt;
  Biology Finance Office&lt;br /&gt;
  77 Massachusetts Avenue, 68-157&lt;br /&gt;
  Cambridge, MA 02139&lt;br /&gt;
&lt;br /&gt;
For a W-9: [https://vpf.mit.edu/forms/7/1220 MIT W-9 Form]&lt;br /&gt;
&lt;br /&gt;
A Technical Services Agreement (TSA) — pre-approved by MIT legal and not open to redlining — is available upon request. Contact [mailto:biomicro@mit.edu biomicro@mit.edu] for details.&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:Submission&amp;diff=131231</id>
		<title>BioMicroCenter:Submission</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:Submission&amp;diff=131231"/>
		<updated>2026-03-26T02:16:20Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: Restore iLabs direct links for Genomics and Informatics services&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;{{BioMicroCenter}}&lt;br /&gt;
&lt;br /&gt;
== Submitting to the BioMicro Center ==&lt;br /&gt;
&lt;br /&gt;
The BioMicro Center accepts samples from MIT-affiliated and external users. For an overview of available services, see the [[BioMicroCenter|home page]].&lt;br /&gt;
&lt;br /&gt;
----&lt;br /&gt;
&lt;br /&gt;
== MIT Users ==&lt;br /&gt;
&lt;br /&gt;
MIT users access the BioMicro Center through [https://mit-ki.ilabsolutions.com/sc/3381/ki-genomics-core-mit-biomicro-center/?tab=about iLabs], which requires an MIT Kerberos account and an active MIT cost object. Kerberos accounts are available to members of MIT, the Whitehead Institute, and qualifying users at the Broad and Ragon Institutes. If you do not have a Kerberos account, see [https://ist.mit.edu/accounts MIT IS&amp;amp;T Accounts].&lt;br /&gt;
&lt;br /&gt;
See [[BioMicroCenter:Pricing|Pricing]] for current rates.&lt;br /&gt;
&lt;br /&gt;
=== Assisted services ===&lt;br /&gt;
&lt;br /&gt;
Assisted services are those where samples are delivered to the BioMicro Center and processed by staff. These include sequencing, library preparation, sample QC, and informatics.&lt;br /&gt;
&lt;br /&gt;
To submit, log in to iLabs and follow these paths:&lt;br /&gt;
&lt;br /&gt;
* [https://mit-ki.ilabsolutions.com/sc/3381/ki-genomics-core-mit-biomicro-center?tab=services &#039;&#039;&#039;Genomics Services&#039;&#039;&#039;] (sequencing, library preparation, sample QC): Request Services → BioMicro Requests → Initiate Request&lt;br /&gt;
* [https://mit-ki.ilabsolutions.com/sc/3189/bioinformatics-and-computing-core/?tab=services &#039;&#039;&#039;Informatics Services&#039;&#039;&#039;] (bioinformatics and computing): Request Services → Initiate Request&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Purchase orders:&#039;&#039;&#039; If you are paying by PO, you must submit with an MIT cost object. Fields for the PO number are included in the submission forms. The MIT cost object is a backup that will only be charged if payment via PO cannot be collected.&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Approval:&#039;&#039;&#039; The BioMicro Center does not hold projects waiting for financial approval. If a submission is not rejected in a timely manner, we will proceed and recharge normally. Please ensure your cost object is active before submitting.&lt;br /&gt;
&lt;br /&gt;
=== Walk-up services ===&lt;br /&gt;
&lt;br /&gt;
Walk-up services are those where users operate equipment themselves after receiving training. To schedule equipment, log in to iLabs and follow this path:&lt;br /&gt;
&lt;br /&gt;
Schedule Equipment → expand the service → View Schedule&lt;br /&gt;
&lt;br /&gt;
Some equipment requires training before sign-up is permitted. Contact [mailto:biomicro@mit.edu biomicro@mit.edu] to arrange training.&lt;br /&gt;
&lt;br /&gt;
After-hours access to the core is part of the walk-up process and requires a one-time form:&lt;br /&gt;
* [https://www.dropbox.com/scl/fi/nxdzbesvp2mab69wofotm/New_User_Sign_Up_Form.docx?rlkey=x9ftkatdlm897fbeol2ibv8tb&amp;amp;st=ag0jsvn9&amp;amp;dl=0 After-Hours Access Form]&lt;br /&gt;
&lt;br /&gt;
=== New labs ===&lt;br /&gt;
&lt;br /&gt;
New labs are set up in iLabs by your PI or a designated administrator — having MIT spending permission does not automatically carry over to iLabs. For assistance getting your lab registered, see [https://ki-sbc.mit.edu/core-access KI Shared Research Core Access] or contact Jack Cimino at the Koch Institute.&lt;br /&gt;
&lt;br /&gt;
----&lt;br /&gt;
&lt;br /&gt;
== External Users ==&lt;br /&gt;
&lt;br /&gt;
The BioMicro Center primarily serves the MIT community. We welcome external users when capacity allows, with the understanding that MIT samples always have priority. &#039;&#039;&#039;The BioMicro Center reserves the right to decline any submission.&#039;&#039;&#039;&lt;br /&gt;
&lt;br /&gt;
See [[BioMicroCenter:Pricing|Pricing]] for current rates.&lt;br /&gt;
&lt;br /&gt;
=== New external labs ===&lt;br /&gt;
&lt;br /&gt;
Each new external lab must complete a one-time form before quotes can be provided:&lt;br /&gt;
* [https://www.dropbox.com/s/tgnmr6lrubprjzx/New_External%20User%20Form_2018.xlsx?dl=0 New External User Form] — required once per lab&lt;br /&gt;
&lt;br /&gt;
=== Assisted services ===&lt;br /&gt;
&lt;br /&gt;
Assisted services are those where samples are delivered to the BioMicro Center and processed by staff.&lt;br /&gt;
&lt;br /&gt;
To submit samples, download the appropriate form below, fill it out, and send it along with your samples. Please email [mailto:biomicro@mit.edu biomicro@mit.edu] in advance to arrange a drop-off date and time.&lt;br /&gt;
&lt;br /&gt;
* [https://www.dropbox.com/scl/fi/7jfss8ofhp98g0doiv9ph/BMC_EXT_shortRead25.xlsx?rlkey=syhww29wpavjakekc2d5lyskl&amp;amp;dl=0 Short Read Library Prep and/or Sequencing] — multipage form; separate tabs for prep and sequencing&lt;br /&gt;
* [https://www.dropbox.com/scl/fi/lgnx0bbfz3lrn00zyplyw/LongReadSeq_User_Form.xlsx?dl=0&amp;amp;rlkey=x1tbksv8swv5xhvp91m68eg42 Long Read Sequencing]&lt;br /&gt;
* [https://www.dropbox.com/s/kuodtqu77079st7/BMC_EXT_FAFP_v4.xlsx?dl=0 Advanced Analytical / Bioanalyzer]&lt;br /&gt;
&lt;br /&gt;
Ship DNA samples at 4°C; ship RNA samples on dry ice. Use overnight delivery and address packages to:&lt;br /&gt;
&lt;br /&gt;
  MIT BioMicro Center&lt;br /&gt;
  31 Ames Street, Building 68-322&lt;br /&gt;
  Cambridge, MA 02139&lt;br /&gt;
&lt;br /&gt;
Please email an electronic copy of your completed order form and PO to [mailto:biomicro@mit.edu biomicro@mit.edu].&lt;br /&gt;
&lt;br /&gt;
=== Walk-up services ===&lt;br /&gt;
&lt;br /&gt;
Walk-up services for external users are generally performed as assisted services, with staff operating the equipment at a nominal fee above the standard equipment rate. This allows scheduling around MIT user demand. After-hours access is not available to external users.&lt;br /&gt;
&lt;br /&gt;
To arrange walk-up access, contact [mailto:biomicro@mit.edu biomicro@mit.edu].&lt;br /&gt;
&lt;br /&gt;
=== Billing ===&lt;br /&gt;
&lt;br /&gt;
Please email purchase orders to [mailto:bio-billing@mit.edu bio-billing@mit.edu].&lt;br /&gt;
&lt;br /&gt;
Direct payment inquiries and checks to:&lt;br /&gt;
&lt;br /&gt;
  Massachusetts Institute of Technology&lt;br /&gt;
  Biology Finance Office&lt;br /&gt;
  77 Massachusetts Avenue, 68-157&lt;br /&gt;
  Cambridge, MA 02139&lt;br /&gt;
&lt;br /&gt;
For a W-9: [https://vpf.mit.edu/forms/7/1220 MIT W-9 Form]&lt;br /&gt;
&lt;br /&gt;
A Technical Services Agreement (TSA) — pre-approved by MIT legal and not open to redlining — is available upon request. Contact [mailto:biomicro@mit.edu biomicro@mit.edu] for details.&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:Forms&amp;diff=131227</id>
		<title>BioMicroCenter:Forms</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:Forms&amp;diff=131227"/>
		<updated>2026-03-26T02:06:29Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: Redirect to BioMicroCenter:Submission — content consolidated there&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;#REDIRECT [[BioMicroCenter:Submission]]&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:FAQ&amp;diff=131226</id>
		<title>BioMicroCenter:FAQ</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:FAQ&amp;diff=131226"/>
		<updated>2026-03-26T02:05:28Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: Remove ILABS and NON MIT USERS sections — content moved to BioMicroCenter:Submission&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;{{BioMicroCenter}}&lt;br /&gt;
&lt;br /&gt;
== CONTACT ==&lt;br /&gt;
&#039;&#039;&#039;STOP BY:&#039;&#039;&#039; The BioMicro Center is located at [http://whereis.mit.edu/?go=68 68-322 on the MIT campus]. &amp;lt;BR&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;EMAIL:&#039;&#039;&#039; The BioMicro [[BioMicroCenter:People|staff]] can be emailed at biomicro@mit.edu &amp;lt;BR&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;PHONE:&#039;&#039;&#039; The main lab number is 617-715-4533 &amp;lt;BR&amp;gt;&lt;br /&gt;
Our director, [[User:Stuart S. Levine|Stuart Levine]], can be reached at 617-452-2949.&lt;br /&gt;
&lt;br /&gt;
== DEFINITIONS ==&lt;br /&gt;
=== CORE LABS ===&lt;br /&gt;
The BioMicro Center is NOT an MIT institutional core, but a departmental resource. Departments that fund the operation of the BioMicro Center are given priority for all resources within the Center. Several labs that have provided major equipment donations to the BioMicro Center are also considered to be CORE. The BioMicro Center is funded by:&lt;br /&gt;
* The Department of Biology &lt;br /&gt;
* The Department of BioEngineering&lt;br /&gt;
* The David H. Koch Institute for Integrative Cancer Research&lt;br /&gt;
&lt;br /&gt;
=== ASSISTED versus WALKUP SERVICES ===&lt;br /&gt;
Services available in the BioMicro Center are offered either as assisted or as walk-up. Walk-up Services are those where the Center provides training and maintenance of equipment and may also provide some consumables. Scheduling for Walk-up services is available through the calendar functions on ilabs and are almost exclusively limited to MIT users except in rare cases. Non-MIT users should contact biomicro@mit.edu to schedule walk-up equipment.&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
Assisted services are those where samples are delivered to the BioMicro Center and are analyzed by Center staff. These are set up using the [[BioMicroCenter:Forms|FORMS]] on this site or the &amp;quot;Request Services&amp;quot; tab in [https://mit.ilabsolutions.com/sc/3381/ki-genomics-core-mit-biomicro-center/?tab=about ilabs]. Assisted services cannot typically be &amp;quot;scheduled&amp;quot; with rare exceptions.&lt;br /&gt;
&lt;br /&gt;
=== STANDARD versus HIGH THROUGHPUT LIBRARY PREPARATION ===&lt;br /&gt;
Standard library preparation is designed to maximize successful production of libraries and is used for routine work and should also be used for all precious samples. Standard library preparation includes initial sample quality control as well as at least one repeat for each library preparation if required due to sample failure. Standard library preparation is charged on a per sample basis. Standard libraries may be prepared by hand or using automation depending on throughput of the core. &amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
&lt;br /&gt;
High Throughput library (HTL) preparation is designed to minimize cost and has many differences from standard library preparation as a result. HTLs costs do NOT include initial sample quality control nor repeats of failures of individual samples. As such, we very strongly discourage HTL library prep for precious samples or experiments with limited replicates (N&amp;lt;=3). Replicate counts of 4 are considered the minimum with 6 being recommended where possible. Some methods, such as HT3DGE, can never repeat failed samples, while others can be reproduced by hand, though that does create a technical variable that can impact analysis. HTLs are almost always produced using liquid handlers, typically using miniaturization, and may require submission of the samples in specific formats (noted on each library prep type). HTLs are spot checked for quality after library production. Full quality control before and after experiments as well as sample arraying and sample cleaning is available at an additional charge.&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
&lt;br /&gt;
We have published a manuscript highlighting some of the methods on [https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/33100919/ JBT].&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
&lt;br /&gt;
A webinar by our director for SPT Labtech [https://www.sptlabtech.com/resources/automated-low-volume-liquid-handling-for-cost-effective-ngs-library-preparation-and-single-cell-genomics-webinar-sign-up can be found here]&lt;br /&gt;
&lt;br /&gt;
== DOWNLOADING DATA ==&lt;br /&gt;
You will be notified by email that your data is ready. The data will be placed on one of our servers for you the download. All data is typically retained for 60 days. We STRONGLY encourage you to keep a local copy of your data as soon as possible. Contact [mailto:bmc-data@mit.edu bmc-data@mit.edu] if you have any issues.&lt;br /&gt;
&lt;br /&gt;
=== MIT / MIT-CERTIFICATE + VPN ===&lt;br /&gt;
{| class=&#039;wikitable&#039;&lt;br /&gt;
 |&#039;&#039;&#039;LURIA&#039;&#039;&#039;||&lt;br /&gt;
* samples can be directly copied from our share /net/bmc-pub17/data/bmc/public/[LAB]/[PROJECT]&lt;br /&gt;
* Please ensure you are running cp from a COMPUTING NODE of Luria instead of the head node&lt;br /&gt;
 |-&lt;br /&gt;
 |&#039;&#039;&#039;Windows/Mac SFTP client||&lt;br /&gt;
* Mac: Fetch | Windows: SecureCRT/FX. Download from [https://ist.mit.edu/software/filetransfer Secure File Transfer at MIT]&lt;br /&gt;
 |-&lt;br /&gt;
 |&#039;&#039;&#039;Web Download &amp;lt;p&amp;gt; (MIT certificate required)&#039;&#039;&#039;||&lt;br /&gt;
https://bmc-data.mit.edu/[LAB]/[PROJECT]&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
=== Globus ===&lt;br /&gt;
Globus is a file transfer service that makes it easy to transfer files between projects and institutions. You&#039;ll need to [https://www.globus.org/get-started register for a Globus account] to use Globus. The data ready e-mail will have a link to your project&#039;s files on Globus, but you can also navigate to them using one of these links:&lt;br /&gt;
&lt;br /&gt;
{| class=&#039;wikitable&#039;&lt;br /&gt;
  |&#039;&#039;&#039;Internal Labs&#039;&#039;&#039;||&lt;br /&gt;
[https://app.globus.org/file-manager/collections/3b0a124f-c273-4150-b383-99ed8c8a2764/overview Koch Institute BioMicro Data (Internal)]&lt;br /&gt;
  |-&lt;br /&gt;
  |&#039;&#039;&#039;External Labs&#039;&#039;&#039;||&lt;br /&gt;
[https://app.globus.org/file-manager/collections/31ca1b0a-1deb-4882-93f0-6e86fe9ebe3a/overview Koch Institute BioMicro Data (External)]&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
If your institution uses Globus, you can transfer your data from our collection to your institution&#039;s collection by following [https://docs.globus.org/guides/tutorials/manage-files/transfer-files/ these instructions.]&lt;br /&gt;
&lt;br /&gt;
Otherwise, if you&#039;d like to download your data to a personal workstation, you can download [https://www.globus.org/globus-connect-personal Globus Connect Personal]. After following the installation instructions, your computer will be available as a collection in Globus, and you can follow [https://docs.globus.org/guides/tutorials/manage-files/transfer-files/ these instructions] to download your data.&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;If you do not have permissions to view or download files, please e-mail [mailto:luria-help@mit.edu luria-help@mit.edu] with your Globus account e-mail, your lab association, and optionally a list of members in the lab who should be granted access to the data as well. Please make sure these members have Globus accounts before sending the e-mail. You can also e-mail [mailto:luria-help@mit.edu luria-help@mit.edu] to have us revoke people&#039;s access.&#039;&#039;&#039;&lt;br /&gt;
&lt;br /&gt;
=== Read archival ===&lt;br /&gt;
&lt;br /&gt;
Users are strongly encouraged to retain a local copy of their data as early as possible after sequencing result delivery. &lt;br /&gt;
&lt;br /&gt;
&amp;lt;b&amp;gt;After 60 days, fastq files will be compressed on the server. Bam files, containing reads aligned either to the genome mentioned in the sample submission form or phiX (a phage genome), will be deleted to save storage space.&amp;lt;/b&amp;gt;&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
&lt;br /&gt;
Older files: Prior to 2020, bam files were maintained as archival files. Fastq files can be regenerated from the bam files (samtools sort -n to sort bam files by read names, and converted back to fastq files using bedtools bamToFastq).&lt;br /&gt;
&lt;br /&gt;
== UPLOADING DATA ==&lt;br /&gt;
&lt;br /&gt;
To upload your data, you can access our file server named bmc-opendata.mit.edu via the sftp protocol. &lt;br /&gt;
&lt;br /&gt;
* Request SFTP account with projects and lab information for storage setup&lt;br /&gt;
* Use a SFTP client (such as WinSCP, FileZilla, PuTTY and Cyberduck for Windows/Mac or sftp command for Linux)&lt;br /&gt;
&lt;br /&gt;
&amp;lt;!---&lt;br /&gt;
== SUBMISSIONS ==&lt;br /&gt;
=== Volume/Concentration ===&lt;br /&gt;
The volume and concentration needed for submission will depend on the type of sample prep.  However, if you are submitting prepped, sequence-ready samples, we require a minimum of 10uL at a concentration of at least 2.0 nM.  This allows us to perform quality control and have enough of the library for clustering.  Any samples less than 2.0 nM cause significant difficulties with clustering the flowcell, which is why we cannot guarantee optimal read count and quality should you like us to proceed with sequencing a low concentration sample.&lt;br /&gt;
&lt;br /&gt;
=== Custom Primers ===&lt;br /&gt;
When submitting [[BioMicroCenter:Sequencing|&#039;&#039;&#039;custom primers&#039;&#039;&#039;]], be sure to verify their compatibility with the standard Illumina Primers used.  If you&#039;d like a second opinion on the compatibility of your primers, we&#039;d be more than happy to sit down with you and verify the primer design based on your constructs.&lt;br /&gt;
&lt;br /&gt;
Another important aspect to consider is the fact that clustering conditions differ when comparing the HiSeq and NextSeq to the MiSeq.  The MiSeq clusters at about 5 degrees higher than either the NextSeq or the cBot, which is used to prepare flowcells for the HiSeq.  This means that all custom primers must be designed appropriately so Tm&#039;s are compatible with whatever sequencing route you choose.&lt;br /&gt;
&lt;br /&gt;
Submit at least 20 microliters at 100 micromolar concentration of each of your primers per each intended lane of sequencing.&lt;br /&gt;
&lt;br /&gt;
== How long will it take for my HiSeq sample to be sequenced? ==&lt;br /&gt;
This is a very hard question to answer because it involves a number of variables. The simplest answer is, sample preparation typically takes ~1 weeks for standard samples with all reagents on hand (QC, prep, QC). For newer or rarely used methods, the number can extend to a month if we need to gather reagents. Once the sample is ready, it goes in to the sequencing queue. This queue is often the longest part of the process. Once the full flowcell is ready and put on a sequencer, it takes ~1.3h per base to sequence (this used to be 1h but upgrades to the machines have slowed them down some). A 40nt read takes just over 2 days. Turn around (for PE samples) functionally takes about a 1/2 day. So a 40+40 PE sample will take about 6 days once it is on the sequencer - if nothing goes wrong. Overall, when the queue is flowing at full speed, a 40nt SE submission with sample preparation can be returned with data in right around 3 weeks. 40+40 PE samples more typically take ~4-6 weeks for [[BioMicroCenter:CoreDeps|CORE lab members]] with the extra time coming from delays in filling out flowcells. &lt;br /&gt;
&lt;br /&gt;
NextSeq and MiSeq are much simpler. Once the sample is prepped and the machine is working, it will be loaded immediately. Total times should be less than 2 weeks.&lt;br /&gt;
&lt;br /&gt;
=== Illumina Queue: Full HiSeq Flowcells === &lt;br /&gt;
The primary requirement for a HiSeq run is a full flowcell. Each flowcell is composed of 7 lanes that must be run together. In the BioMicro Center, lanes are grouped by read length to optimize throughput and keep costs low. This requirement for 7 samples has a major impact on queue time. If you do a common read length, such as 40nt SE, the queue length will be short. On the other hand, if you ask for an unusual read length, your samples may never come off the queue. By frequency, the most common read lengths are, in order: &#039;&#039;&#039;40SE, 50SE, and 40+40PE&#039;&#039;&#039;. We do fudge a little in some of the long read lengths to fit samples in (mixing 40 and 50 for example), but we will always give priority to the samples of the read length we are going to do. We do not run incomplete flowcells and other options, such as NextSeq and MiSeq exist for unusual read lengths. NOTE: Barcoding is assumed to be done on all flowcells and we do NOT restrict pooling based on which samples are multiplexed and which are not.&lt;br /&gt;
&lt;br /&gt;
=== Illumina Queue: Priority ===&lt;br /&gt;
{|&lt;br /&gt;
 |width=450|&lt;br /&gt;
The BioMicro Center queue operates on a tiered priority basis (see table). We hold to this priority schema as closely as we can while still needing to fill flowcells. Some caveats: Once a flowcell is quality controlled and &amp;quot;on deck&amp;quot; for clustering, that flowcell is considered locked and no new submissions will be added to it. Clustering can happen a week or more before loading. Once flowcells are clustered, their order is set barring the need for reruns.&lt;br /&gt;
 |&lt;br /&gt;
{| align=&amp;quot;right&amp;quot;, textalign=&amp;quot;center&amp;quot;, border=1 &lt;br /&gt;
 ! Priority&lt;br /&gt;
 ! Type&lt;br /&gt;
 |-&lt;br /&gt;
 | 1&lt;br /&gt;
 | Reruns of failures&lt;br /&gt;
 |-&lt;br /&gt;
 | 2&lt;br /&gt;
 | Samples from labs that bought the equipment &lt;br /&gt;
 |-&lt;br /&gt;
 | 3&lt;br /&gt;
 | [[BioMicroCenter:CoreDeps|MIT CORE labs]]&lt;br /&gt;
 |-&lt;br /&gt;
 | 4&lt;br /&gt;
 | MIT non-CORE labs&lt;br /&gt;
 |-&lt;br /&gt;
 | 5&lt;br /&gt;
 | non-MIT users&lt;br /&gt;
|}&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
=== Failures ===&lt;br /&gt;
The Illumina HiSeq is temperamental beast and failures do happen. The large majority of the failures on our sequencer are due to equipment failure and not due to operator failure, although those do happen as well. Once a failed run is identified, we must work with Illumina to identify the cause and prove to them that the cause is equipment based. This allows us to get replacement reagents which helps keep your costs down. Once a run has failed, the failed samples on that flowcell are given highest priority. &lt;br /&gt;
&lt;br /&gt;
Failures create a large challenge for us in providing estimates of when your data will be ready. Because the failure rate is so high and failures tend to cluster, short sequencing estimates can quickly turn in to long waits, particularly if you are not at the top of the priority queue. The BioMicro Center works closely with the KI Biopolymer Core and the Whitehead Institute GTC to move samples to other machines whenever possible if we develop a backlog. &lt;br /&gt;
&lt;br /&gt;
=== Triage ===&lt;br /&gt;
The final consideration in timing is best summarized as &amp;quot;Triage&amp;quot;. This encompasses all of the many other factors we take in to account in creating flowcells. This can range from scheduling: Pushing a 40nt SE run on before an 40+40PE run so the turn around of the PE times to be during the week instead of over a weekend - to flowcell logistics: Grabbing low priority 40SE samples to fill out a rerun flowcell while an 40+40PE run that does not have rerun samples waits in the queue. Our goal is to get everyone their data as fast as we possibly can. We look at every project every day and we are working as hard as we can to get you your data as fast as we can.&lt;br /&gt;
--&amp;gt;&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:Submission&amp;diff=131225</id>
		<title>BioMicroCenter:Submission</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:Submission&amp;diff=131225"/>
		<updated>2026-03-26T02:03:32Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: Create Submission page consolidating Forms and FAQ submission content&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;{{BioMicroCenter}}&lt;br /&gt;
&lt;br /&gt;
== Submitting to the BioMicro Center ==&lt;br /&gt;
&lt;br /&gt;
The BioMicro Center accepts samples from MIT-affiliated and external users. For an overview of available services, see the [[BioMicroCenter|home page]].&lt;br /&gt;
&lt;br /&gt;
----&lt;br /&gt;
&lt;br /&gt;
== MIT Users ==&lt;br /&gt;
&lt;br /&gt;
MIT users access the BioMicro Center through [https://mit-ki.ilabsolutions.com/sc/3381/ki-genomics-core-mit-biomicro-center/?tab=about iLabs], which requires an MIT Kerberos account and an active MIT cost object. Kerberos accounts are available to members of MIT, the Whitehead Institute, and qualifying users at the Broad and Ragon Institutes. If you do not have a Kerberos account, see [https://ist.mit.edu/accounts MIT IS&amp;amp;T Accounts].&lt;br /&gt;
&lt;br /&gt;
See [[BioMicroCenter:Pricing|Pricing]] for current rates.&lt;br /&gt;
&lt;br /&gt;
=== Assisted services ===&lt;br /&gt;
&lt;br /&gt;
Assisted services are those where samples are delivered to the BioMicro Center and processed by staff. These include sequencing, library preparation, sample QC, and informatics.&lt;br /&gt;
&lt;br /&gt;
To submit, log in to iLabs and follow these paths:&lt;br /&gt;
&lt;br /&gt;
* &#039;&#039;&#039;Genomics&#039;&#039;&#039; (sequencing, library preparation, sample QC): Request Services → BioMicro Requests → Initiate Request&lt;br /&gt;
* &#039;&#039;&#039;Informatics&#039;&#039;&#039; (bioinformatics and computing): Request Services → Initiate Request&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Purchase orders:&#039;&#039;&#039; If you are paying by PO, you must submit with an MIT cost object. Fields for the PO number are included in the submission forms. The MIT cost object is a backup that will only be charged if payment via PO cannot be collected.&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Approval:&#039;&#039;&#039; The BioMicro Center does not hold projects waiting for financial approval. If a submission is not rejected in a timely manner, we will proceed and recharge normally. Please ensure your cost object is active before submitting.&lt;br /&gt;
&lt;br /&gt;
=== Walk-up services ===&lt;br /&gt;
&lt;br /&gt;
Walk-up services are those where users operate equipment themselves after receiving training. To schedule equipment, log in to iLabs and follow this path:&lt;br /&gt;
&lt;br /&gt;
Schedule Equipment → expand the service → View Schedule&lt;br /&gt;
&lt;br /&gt;
Some equipment requires training before sign-up is permitted. Contact [mailto:biomicro@mit.edu biomicro@mit.edu] to arrange training.&lt;br /&gt;
&lt;br /&gt;
After-hours access to the core is part of the walk-up process and requires a one-time form:&lt;br /&gt;
* [https://www.dropbox.com/scl/fi/nxdzbesvp2mab69wofotm/New_User_Sign_Up_Form.docx?rlkey=x9ftkatdlm897fbeol2ibv8tb&amp;amp;st=ag0jsvn9&amp;amp;dl=0 After-Hours Access Form]&lt;br /&gt;
&lt;br /&gt;
=== New labs ===&lt;br /&gt;
&lt;br /&gt;
New labs are set up in iLabs by your PI or a designated administrator — having MIT spending permission does not automatically carry over to iLabs. For assistance getting your lab registered, see [https://ki-sbc.mit.edu/core-access KI Shared Research Core Access] or contact Jack Cimino at the Koch Institute.&lt;br /&gt;
&lt;br /&gt;
----&lt;br /&gt;
&lt;br /&gt;
== External Users ==&lt;br /&gt;
&lt;br /&gt;
The BioMicro Center primarily serves the MIT community. We welcome external users when capacity allows, with the understanding that MIT samples always have priority. &#039;&#039;&#039;The BioMicro Center reserves the right to decline any submission.&#039;&#039;&#039;&lt;br /&gt;
&lt;br /&gt;
See [[BioMicroCenter:Pricing|Pricing]] for current rates.&lt;br /&gt;
&lt;br /&gt;
=== New external labs ===&lt;br /&gt;
&lt;br /&gt;
Each new external lab must complete a one-time form before quotes can be provided:&lt;br /&gt;
* [https://www.dropbox.com/s/tgnmr6lrubprjzx/New_External%20User%20Form_2018.xlsx?dl=0 New External User Form] — required once per lab&lt;br /&gt;
&lt;br /&gt;
=== Assisted services ===&lt;br /&gt;
&lt;br /&gt;
Assisted services are those where samples are delivered to the BioMicro Center and processed by staff.&lt;br /&gt;
&lt;br /&gt;
To submit samples, download the appropriate form below, fill it out, and send it along with your samples. Please email [mailto:biomicro@mit.edu biomicro@mit.edu] in advance to arrange a drop-off date and time.&lt;br /&gt;
&lt;br /&gt;
* [https://www.dropbox.com/scl/fi/7jfss8ofhp98g0doiv9ph/BMC_EXT_shortRead25.xlsx?rlkey=syhww29wpavjakekc2d5lyskl&amp;amp;dl=0 Short Read Library Prep and/or Sequencing] — multipage form; separate tabs for prep and sequencing&lt;br /&gt;
* [https://www.dropbox.com/scl/fi/lgnx0bbfz3lrn00zyplyw/LongReadSeq_User_Form.xlsx?dl=0&amp;amp;rlkey=x1tbksv8swv5xhvp91m68eg42 Long Read Sequencing]&lt;br /&gt;
* [https://www.dropbox.com/s/kuodtqu77079st7/BMC_EXT_FAFP_v4.xlsx?dl=0 Advanced Analytical / Bioanalyzer]&lt;br /&gt;
&lt;br /&gt;
Ship DNA samples at 4°C; ship RNA samples on dry ice. Use overnight delivery and address packages to:&lt;br /&gt;
&lt;br /&gt;
  MIT BioMicro Center&lt;br /&gt;
  31 Ames Street, Building 68-322&lt;br /&gt;
  Cambridge, MA 02139&lt;br /&gt;
&lt;br /&gt;
Please email an electronic copy of your completed order form and PO to [mailto:biomicro@mit.edu biomicro@mit.edu].&lt;br /&gt;
&lt;br /&gt;
=== Walk-up services ===&lt;br /&gt;
&lt;br /&gt;
Walk-up services for external users are generally performed as assisted services, with staff operating the equipment at a nominal fee above the standard equipment rate. This allows scheduling around MIT user demand. After-hours access is not available to external users.&lt;br /&gt;
&lt;br /&gt;
To arrange walk-up access, contact [mailto:biomicro@mit.edu biomicro@mit.edu].&lt;br /&gt;
&lt;br /&gt;
=== Billing ===&lt;br /&gt;
&lt;br /&gt;
Please email purchase orders to [mailto:bio-billing@mit.edu bio-billing@mit.edu].&lt;br /&gt;
&lt;br /&gt;
Direct payment inquiries and checks to:&lt;br /&gt;
&lt;br /&gt;
  Massachusetts Institute of Technology&lt;br /&gt;
  Biology Finance Office&lt;br /&gt;
  77 Massachusetts Avenue, 68-157&lt;br /&gt;
  Cambridge, MA 02139&lt;br /&gt;
&lt;br /&gt;
For a W-9: [https://vpf.mit.edu/forms/7/1220 MIT W-9 Form]&lt;br /&gt;
&lt;br /&gt;
A Technical Services Agreement (TSA) — pre-approved by MIT legal and not open to redlining — is available upon request. Contact [mailto:biomicro@mit.edu biomicro@mit.edu] for details.&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:SingleCell&amp;diff=131224</id>
		<title>BioMicroCenter:SingleCell</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:SingleCell&amp;diff=131224"/>
		<updated>2026-03-24T20:29:09Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: Update AVITI24 hidden comment: high-output DISS not yet released&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;{{BioMicroCenter}}&lt;br /&gt;
 &lt;br /&gt;
[[File:SingleCellOptions.png|thumb|right|400px]]&lt;br /&gt;
The BioMicro Center supports a broad range of methods for single cell sequencing. The choice of method depends heavily on the type of question being asked and the source material. The methods break down into those supporting individual cells characterized in single wells and methods that use droplet isolation and library preparation. These cell requirements for each method vary significantly so reviewing each method is valuable and we strongly encourage consultation with BMC staff prior to beginning the experiment.&lt;br /&gt;
&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;.&lt;br /&gt;
 &lt;br /&gt;
 &lt;br /&gt;
== 10x CHROMIUM X ==&lt;br /&gt;
[[File:25_10xAssays.png|thumb|left|400px]]&lt;br /&gt;
The BioMicro Center provides access to 10x Genomics library preparation as an assisted or a walk-up service. Added in 2023, The 10x Chromium X can handle a broad variety of methodologies now including [https://www.10xgenomics.com/solutions/single-cell/ 3&#039;, 5&#039;  and fixed RNA sequencing], [https://www.10xgenomics.com/solutions/single-cell-atac/ ATAC and multiome] &amp;lt;!-- [https://www.10xgenomics.com/solutions/single-cell-cnv/ CNV]. --&amp;gt; Dropoff for assisted service is coordinated with Center staff with at least one week&#039;s lead time. Users should bring their single cell/nuclei suspension(s) in 1.5 mL microfuge tubes in the standard buffer at or around that time. Staff works with the user to intake the initial samples and proceed through the protocol with quality control checks at the appropriate steps. &lt;br /&gt;
&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
Chromium X usage is also offered as a walkup service. Usage may be scheduled on the iLabs calendar after training by BMC staff. This still requires at least a week&#039;s lead time before usage. You will not have permission to schedule the equipment until you have been approved by BMC staff. Reagents are stocked in the BioMicro Center and we ask that you use our reagents. This allows us to get larger bulk discounts we can pass on to our users. Please note that we do include a fraction of the instrument usage cost in the cost of the chips and will charge this cost if you use your own chips.&lt;br /&gt;
&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
The full 10x suite of software is installed on LURIA and is integrated into our analysis package. &amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
{|&lt;br /&gt;
 |- style=&amp;quot;vertical-align: top;&amp;quot;&lt;br /&gt;
 |style=&amp;quot;width: 450px;&amp;quot;|&lt;br /&gt;
=== scRNAseq / snRNAseq ===&lt;br /&gt;
&amp;lt;!-- A &amp;quot;setup&amp;quot; covers the chip, labor, and service contract. For Flex it also includes the GEMs. Samples per setup differ by assay - see Samples per setup row. --&amp;gt;&lt;br /&gt;
 {| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
  !   &lt;br /&gt;
  ! 3&#039;RNA/5&#039;RNA &amp;lt;BR&amp;gt; (GEM-X)&lt;br /&gt;
  ! 3&#039;/5&#039; OnChipMultiplexing &amp;lt;BR&amp;gt; (OCM)&lt;br /&gt;
  ! Fixed RNA &amp;lt;BR&amp;gt; (Flex v2 / Apex)&lt;br /&gt;
  |-&lt;br /&gt;
  !INPUT &lt;br /&gt;
  |colspan=&amp;quot;2&amp;quot;|&lt;br /&gt;
[https://www.10xgenomics.com/support/universal-three-prime-gene-expression/documentation/steps/sample-prep Freshly counted cells/nuclei in suspension.]&lt;br /&gt;
  |&lt;br /&gt;
[https://www.10xgenomics.com/support/flex-gene-expression/documentation/steps/sample-prep Fixed cells in suspension.] Also supports fresh, frozen, FFPE, fixed whole blood, and flow-sorted cells.&lt;br /&gt;
  |-&lt;br /&gt;
  !Cell Concentration &amp;lt;BR&amp;gt;&amp;lt;small&amp;gt; cells|nuclei/uL &amp;lt;/small&amp;gt;&lt;br /&gt;
  |100-2000&lt;br /&gt;
  |100-2000&lt;br /&gt;
  |100-2000&lt;br /&gt;
  |-&lt;br /&gt;
  ! Fraction Recovered &amp;lt;BR&amp;gt;&amp;lt;small&amp;gt; est. percent of input cells captured &amp;lt;/small&amp;gt;&lt;br /&gt;
  | ~65%&lt;br /&gt;
  | ~60%&lt;br /&gt;
  | ~50%&lt;br /&gt;
  |-&lt;br /&gt;
  ! Target recovery &amp;lt;BR&amp;gt; &amp;lt;small&amp;gt; multiply by recovery for input &amp;lt;/small&amp;gt;&lt;br /&gt;
  | 500-20,000/lane&lt;br /&gt;
  | 500-5,000/lane &lt;br /&gt;
  | up to 20,000/sample&lt;br /&gt;
  |-&lt;br /&gt;
  ! Expected doublet rate @ Max load&lt;br /&gt;
  | 8%&lt;br /&gt;
  | 7.5%&lt;br /&gt;
  | 8% doublet @ 20k&lt;br /&gt;
  |-&lt;br /&gt;
  ! Samples per setup &amp;lt;BR&amp;gt;&amp;lt;small&amp;gt; A setup covers chip, labor, and service contract. Flex also includes GEMs. &amp;lt;/small&amp;gt;&lt;br /&gt;
  | 1-8 samples &amp;lt;BR&amp;gt;&amp;lt;small&amp;gt; 8 GEMs per chip &amp;lt;/small&amp;gt;&lt;br /&gt;
  | 1-8 samples &amp;lt;BR&amp;gt;&amp;lt;small&amp;gt; 4 lanes/batch → 2 GEMs per chip &amp;lt;/small&amp;gt;&lt;br /&gt;
  | up to ~48 samples &amp;lt;BR&amp;gt;&amp;lt;small&amp;gt; 1 GEM; ~1M cells captured; ~20k cells/index &amp;lt;/small&amp;gt;&lt;br /&gt;
  |-&lt;br /&gt;
  !ADD&#039;L AMPLICONS&lt;br /&gt;
  |colspan=&amp;quot;2&amp;quot; |&lt;br /&gt;
Custom priming for CITEseq, CRISPRs (perturb-seq), TCR (5&#039;), BCR (5&#039;). &amp;lt;BR&amp;gt; Please provide custom oligos and protocols to core. &lt;br /&gt;
  |&lt;br /&gt;
Additional genes [https://www.10xgenomics.com/support/flex-gene-expression/documentation/steps/experimental-design-and-planning/custom-probe-design-for-visium-spatial-gene-expression-and-chromium-single-cell-gene-expression-flex require new probes.]&lt;br /&gt;
  |-&lt;br /&gt;
  ! KEY NOTES&lt;br /&gt;
  |&lt;br /&gt;
  | Batches of 4 samples&lt;br /&gt;
  | Human/Mouse only. &amp;lt;BR&amp;gt;&amp;lt;small&amp;gt; Probe-based: exogenous sequences (GFP, reporters, etc.) not captured without custom probes. &amp;lt;/small&amp;gt;&lt;br /&gt;
  |-&lt;br /&gt;
  !SUBMISSION FORMS&lt;br /&gt;
  |colspan=&amp;quot;3&amp;quot;| ASSISTED - [https://mit.ilabsolutions.com/service_item/new/3381?spt_id=12903 ilabs]  WALKUP - [https://mit.ilabsolutions.com/equipment/380963 Calendar]&lt;br /&gt;
  |-&lt;br /&gt;
  !DELIVERY &lt;br /&gt;
  |colspan=&amp;quot;3&amp;quot;| FASTQ, SAM, BAM, 10X QC, loupe file&lt;br /&gt;
  |-&lt;br /&gt;
  !DONATED BY &lt;br /&gt;
  |colspan=&amp;quot;3&amp;quot;| [http://mit.edu/manoli/ Prof. Manolis Kellis]&lt;br /&gt;
 |}&lt;br /&gt;
 &lt;br /&gt;
=== CHROMATIN ===&lt;br /&gt;
 {| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
  !   &lt;br /&gt;
  ! snATACseq&lt;br /&gt;
  ! multiome&lt;br /&gt;
  |-&lt;br /&gt;
  ! Input&lt;br /&gt;
  | colspan=2 |&lt;br /&gt;
* 160-8,000 nuclei/uL&lt;br /&gt;
* 50% estimated recovery&lt;br /&gt;
* 7.5% doublets @ 10k cells recovered&lt;br /&gt;
* 10uL minimum volume&lt;br /&gt;
 |-&lt;br /&gt;
 ! Multiplexing&lt;br /&gt;
 | colspan =2 | Not available&lt;br /&gt;
 |-&lt;br /&gt;
  !SUBMISSION FORMS&lt;br /&gt;
  |colspan=&amp;quot;2&amp;quot;| ASSISTED - [https://mit.ilabsolutions.com/service_item/new/3381?spt_id=12903 ilabs]  WALKUP - [https://mit.ilabsolutions.com/equipment/380963 Calendar]&lt;br /&gt;
  |-&lt;br /&gt;
  !DELIVERY &lt;br /&gt;
  |colspan=&amp;quot;2&amp;quot;| FASTQ, BAM, 10X QC, loupe file&lt;br /&gt;
 |}&lt;br /&gt;
 &lt;br /&gt;
|&lt;br /&gt;
 &lt;br /&gt;
=== 10X Genomics ===&lt;br /&gt;
[[image:10xX.jpg|thumb|right|500px|10x Chromium X]]&lt;br /&gt;
 &lt;br /&gt;
 &lt;br /&gt;
FAQs for Users &amp;lt;BR&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;1) What buffers in the final suspension are compatible with 10X applications?&#039;&#039;&#039; &lt;br /&gt;
&amp;lt;br&amp;gt;&lt;br /&gt;
[https://kb.10xgenomics.com/s/article/115001937123-What-buffers-can-be-used-for-washing-and-cell-resuspension Buffers/media] for the submitted single cell/nuclei suspension should not contain excessive amounts of EDTA (&amp;gt;0.1 mM) or magnesium (&amp;gt;3 mM) and should be free of surfactants (i.e. Tween-20, SDS etc) and any RNases or DNases.&lt;br /&gt;
*1xPBS (calcium free and magnesium free) containing 0.04%  weight/volume BSA (400 µg/ml) is recommended for most general protocols and is considered the standard buffer&lt;br /&gt;
*Cell culture media with up to 1% BSA or up to 10% FBS if cells are not viable in standard buffer&lt;br /&gt;
*1xPBS (calcium free and magnesium free) containing up to 10% FBS for CMO labelling&lt;br /&gt;
*Nuclei also require addition of RNase Inhibitor along with 10X Genomics 1X nuclei buffer before chip loading, instructions for which are included in 10x user guides. If needed, users can collect buffer aliquots from the BMC after submitting a project. Please coordinate with BMC staff for pick-up. &amp;lt;br&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;2) How many cells are captured in the Assay?&#039;&#039;&#039;&lt;br /&gt;
&amp;lt;br&amp;gt;Up to 20,000 cells per GEM can be uniquely barcoded with GEM-X, but this highly depends on cell counts and viability. Dying cells will leak RNA, hence may not be captured efficiently leading to sample failures. We recommend to count cells at the BMC to avoid discrepancies, but can work with users&#039; counts as well. &amp;lt;BR&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;3) What are the best practices for flow sorting cells?&#039;&#039;&#039; &amp;lt;br&amp;gt; &lt;br /&gt;
10x provides guidance with their tested protocols about pre-sort buffer, collection buffer and FACS best practices [https://kb.10xgenomics.com/s/article/360048826911-What-are-the-best-practices-for-flow-sorting-cells-for-10x-Genomics-assays here.] &amp;lt;br&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;4) What is the expected size distribution for cDNA?&#039;&#039;&#039; &amp;lt;br&amp;gt;&lt;br /&gt;
cDNA for 3&#039; and 5&#039; libraries will span between 400 to 9000 base pairs, depending on sample type.&lt;br /&gt;
&amp;lt;br&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;5) How much sequencing per sample is recommended?&#039;&#039;&#039; &amp;lt;br&amp;gt;&lt;br /&gt;
10x makes several recommendations in their [https://www.10xgenomics.com/support/epi-atac/documentation/steps/sequencing/sequencing-handbook sequencing handbook]. Recommendation numbers vary by sample type, expected CNPs per sample, assay type and general sample quality. The higher the CNPs, the higher the quality, the more likely increased read depth is required. &amp;lt;br&amp;gt;&lt;br /&gt;
For more resources, please visit https://kb.10xgenomics.com/s/ &amp;lt;br&amp;gt;&lt;br /&gt;
|&lt;br /&gt;
|}&lt;br /&gt;
 &lt;br /&gt;
== NAMOCELL SINGLE CELL SORTER ==&lt;br /&gt;
 &lt;br /&gt;
{| align=left &lt;br /&gt;
 |- style=&amp;quot;vertical-align: top;&amp;quot;&lt;br /&gt;
 |style=&amp;quot;width: 300px;&amp;quot;|&lt;br /&gt;
 {| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
  ! INSTRUMENT|| NAMOCELL SINGLE CELL SORTER&lt;br /&gt;
  |-&lt;br /&gt;
  | TYPE || WALKUP - MIT only &amp;lt;BR&amp;gt; FACS&lt;br /&gt;
  |-&lt;br /&gt;
  | UNIT || Per Cartridge&lt;br /&gt;
  |-&lt;br /&gt;
  | LASER WAVELENGTH	||  488 nm&lt;br /&gt;
  |-&lt;br /&gt;
  | DETECTION CHANNELS&lt;br /&gt;
  |&lt;br /&gt;
* FL1 533 nm (FITC/GFP)&lt;br /&gt;
* FL2 585 nm (PE/PI)&lt;br /&gt;
* FL3 676 nm (PerCP)&lt;br /&gt;
  |-&lt;br /&gt;
  | DISPENSE VOLUME || 1 ul&lt;br /&gt;
  |-&lt;br /&gt;
  | SAMPLE VOLUME || 100-750 ul&lt;br /&gt;
  |-&lt;br /&gt;
  | CELL INPUT || single cell mode: 100-10,000 cells &lt;br /&gt;
  |-&lt;br /&gt;
  | FORMAT || 96w/384w&lt;br /&gt;
  |-&lt;br /&gt;
  | SIGNUP || [https://mit.ilabsolutions.com/equipment/show/370751/?tab=schedule ILABS]&lt;br /&gt;
  |-&lt;br /&gt;
  | NEW USERS ||  New users should request training by emailing biomicro@mit.edu&lt;br /&gt;
  |-&lt;br /&gt;
  | DONATED BY || Prof Linda Griffith&lt;br /&gt;
 |}&lt;br /&gt;
 |&lt;br /&gt;
[[image:Namo.jpg|thumb|right|Namo (Namocell) Bio-Techne]]&lt;br /&gt;
The [https://www.bio-techne.com/brands/namocell Namocell Single Cell Sorter] allows users to sort cells into plates. The sorter uses microfluidics to sort single cells in 1uL of sheath fluid into a well. The instrument uses disposable cartridges to minimize contamination. The instrument integrates well with the [[BioMicroCenter:Tecan_Freedom_Evo#SPT_LABTECH_MOSQUITO_HV|SPT Labtech Mosquito HV]] which handles small reaction volumes. &amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
|} &lt;br /&gt;
&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;.&lt;br /&gt;
 &lt;br /&gt;
&amp;lt;!-- AVITI24 high-output DISS (whole transcriptome single cell) section pending - not yet released --&amp;gt;&lt;br /&gt;
 &lt;br /&gt;
&amp;lt;!-- commenting out Seq-Well &lt;br /&gt;
== SEQ-WELL ==&lt;br /&gt;
{|&lt;br /&gt;
 |- style=&amp;quot;vertical-align: top;&amp;quot;&lt;br /&gt;
 |style=&amp;quot;width: 300px;&amp;quot;|&lt;br /&gt;
 {| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
  !   &lt;br /&gt;
  !SEQ-WELL &lt;br /&gt;
  |-&lt;br /&gt;
  |INPUT&lt;br /&gt;
  |&lt;br /&gt;
* FULL PREP: &amp;lt;BR&amp;gt;50,000 single free cells. 90% viability&lt;br /&gt;
* EXO1 and AMP &amp;lt;BR&amp;gt; Tubes with cDNA attached to beads&lt;br /&gt;
* LIBRARY ONLY &amp;lt;BR&amp;gt; Amplified cDNA&lt;br /&gt;
  |-&lt;br /&gt;
  |THROUGHPUT &lt;br /&gt;
  |&lt;br /&gt;
* FULL PREP/EXO: 4 samples/day&lt;br /&gt;
* LIBRARY: 24 samples/day&lt;br /&gt;
  |-&lt;br /&gt;
  |INCLUDED || QC at cDNA stage, library generation.&lt;br /&gt;
  |-&lt;br /&gt;
  |RECOMMENDED SEQUENCING || NextSeq500 - 75nt kit - 2-4 samples/flowcell&lt;br /&gt;
  |-&lt;br /&gt;
  |SUBMISSION || MIT - [https://mit.ilabsolutions.com/service_item/new/3381?spt_id=12903 ilabs] &amp;lt;BR&amp;gt; External contact biomicro@mit.edu&lt;br /&gt;
  |-&lt;br /&gt;
  |DELIVERY || FASTQ, SAM, BAM, Cell:Gene matrix&lt;br /&gt;
  |-&lt;br /&gt;
  |UNIT || PER ARRAY&lt;br /&gt;
 |}&lt;br /&gt;
 |&lt;br /&gt;
The BioMicro Center collaborates closely with the Nanowell Core in the Koch Institute to provide support for the [https://shaleklab.com/resources/seq-well/ Seq-Well] protocol developed by Drs. Alex Shalek and Chris Love&#039;s groups. Seq-Well is based on the isolation of individual cells into microwells where cells are lysed and the associated mRNA is attached to polydT containing beads.  &amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
Seq-Well projects can be brought in two ways. For full service, please use the ilabs form linked on the left. At the end of the form it asks you to select several days on which the experiment can be done. The Nanowell Core will review the possible dates with you to fit it in the schedule. On that date, the core will confirm the quality of your cells prior to beginning the experiment. While 10,000 cells are typically used in a Seq-Well experiment, the core requests you plan to bring more cells - often counts from flow cytometers and other instruments are incorrect. Planning to bring more cells ensures you have the 10,000 desired. The BioMicro Center becomes involved in these projects at the exonuclease treatment step, where we take over the preparation of the library and quality control, as well as sequencing and analysis.&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
Many laboratories are also using reagents from the Nanowell core to begin doing Seq-Well on their own. For these labs, we strongly recommend at least confirming the quality of the cDNA on our Advanced Analytical before continuing through library preparation. The library preparation can also be done in the BioMicro Center. Please use the standard Illumina library preparation form and note that the samples are Seq-Well. We do have all the custom primers needed for preparation and the pricing is identical to other NexteraXT preps. &lt;br /&gt;
|}&lt;br /&gt;
--&amp;gt;&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:SpTx&amp;diff=131223</id>
		<title>BioMicroCenter:SpTx</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:SpTx&amp;diff=131223"/>
		<updated>2026-03-24T19:47:10Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: Remove stale pricing/workflow language from AVITI24 beta banner and pricing paragraph&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;{{BioMicroCenter}}&lt;br /&gt;
 &lt;br /&gt;
The BioMicro Center supports two complementary approaches to spatial transcriptomics: [[BioMicroCenter:SpTx#10X Visium HD|10x Visium HD]] from 10x Genomics for slide-based spatial gene expression, and [[BioMicroCenter:SpTx#AVITI24 In Situ Sequencing|AVITI24 in situ sequencing]] from Element Biosciences. The choice of method depends on your tissue type, resolution requirements, and experimental goals. We strongly encourage consultation with BMC staff prior to beginning a spatial experiment.&lt;br /&gt;
 &lt;br /&gt;
== 10X VISIUM HD ==&lt;br /&gt;
{| width=&amp;quot;100%&amp;quot;&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;width: 200px;&amp;quot;|&lt;br /&gt;
[[File:VisiumHD_workflow_slide.jpg|left|250px|Workflow and capture areas for Visium HD]]&lt;br /&gt;
&amp;lt;div style=&amp;quot;clear:both; padding-top:4px; font-size:12px; color:#555;&amp;quot;&amp;gt;Workflow and capture areas for Visium HD&amp;lt;/div&amp;gt;&lt;br /&gt;
|&lt;br /&gt;
[https://www.10xgenomics.com/products/visium-hd-spatial-gene-expression 10x Visium HD] is a spatial transcriptomics platform that profiles whole-transcriptome gene expression across intact tissue sections at single-cell-scale resolution. Tissue sections are processed through the CytAssist instrument, which transfers RNA complexes onto a capture slide containing a continuous array of 3,000,000 spatially barcoded 2 µm squares covering a 6.5 × 6.5 mm capture area (~0.65 cm²). Two chemistries are available: a probe-based assay for human and mouse (FF, FxF, and FFPE), and a polyA-based assay (Visium HD 3&#039;) for fresh frozen tissue from any species. H&amp;amp;E or immunofluorescence (IF) staining can be performed prior to processing for protein co-detection.&lt;br /&gt;
&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
|}&lt;br /&gt;
 &lt;br /&gt;
{|&lt;br /&gt;
 |- style=&amp;quot;vertical-align: top;&amp;quot;&lt;br /&gt;
 |style=&amp;quot;width: 500px;&amp;quot;|&lt;br /&gt;
 &lt;br /&gt;
=== ARRAY SPECIFICATIONS ===&lt;br /&gt;
 {| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
  ! Capture area&lt;br /&gt;
  | 6.5 × 6.5 mm (~0.65 cm²) per tissue&lt;br /&gt;
  |-&lt;br /&gt;
  ! Spots&lt;br /&gt;
  | 3,000,000 barcoded squares&lt;br /&gt;
  |-&lt;br /&gt;
  ! Spot size&lt;br /&gt;
  | 2 µm continuous (no gaps)&lt;br /&gt;
  |-&lt;br /&gt;
  ! Default analysis bins&lt;br /&gt;
  | 8 µm and 16 µm (SpaceRanger); custom bins supported&lt;br /&gt;
  |-&lt;br /&gt;
  ! Tissues per slide&lt;br /&gt;
  | 2&lt;br /&gt;
  |-&lt;br /&gt;
  ! Multiomics&lt;br /&gt;
  | H&amp;amp;E or IF staining prior to processing; antibody panels (FFPE, human only)&lt;br /&gt;
 |}&lt;br /&gt;
 &lt;br /&gt;
=== CHEMISTRY OPTIONS ===&lt;br /&gt;
 {| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
  !&lt;br /&gt;
  ! Visium HD (probe-based)&lt;br /&gt;
  ! Visium HD 3&#039; (polyA-based)&lt;br /&gt;
  |-&lt;br /&gt;
  ! Species&lt;br /&gt;
  | Human, Mouse only&lt;br /&gt;
  | Any species&lt;br /&gt;
  |-&lt;br /&gt;
  ! Tissue formats&lt;br /&gt;
  | Fresh Frozen (FF), Fixed Frozen (FxF), FFPE&lt;br /&gt;
  | Fresh Frozen (FF) only&lt;br /&gt;
  |-&lt;br /&gt;
  ! Notes&lt;br /&gt;
  | ~3 probes per gene; exogenous sequences (GFP, reporters) require custom probes. A small fraction of genes are excluded by default due to predicted off-target activity: [https://www.10xgenomics.com/support/spatial-gene-expression-hd/documentation/steps/probe-sets/visium-human-transcriptome-probe-set-v2-1 human excluded gene list] (~2.1%), [https://www.10xgenomics.com/support/spatial-gene-expression-hd/documentation/steps/probe-sets/visium-mouse-transcriptome-probe-set-v2-0 mouse excluded gene list] (~1.8%).&lt;br /&gt;
  | Species-agnostic; captures polyadenylated transcripts&lt;br /&gt;
 |}&lt;br /&gt;
 &lt;br /&gt;
=== TISSUE PREPARATION REQUIREMENTS ===&lt;br /&gt;
 {| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
  !&lt;br /&gt;
  ! Fresh Frozen (FF)&lt;br /&gt;
  ! Fixed Frozen (FxF)&lt;br /&gt;
  ! FFPE&lt;br /&gt;
  |-&lt;br /&gt;
  ! Section thickness&lt;br /&gt;
  | 10–20 µm &amp;lt;BR&amp;gt;&amp;lt;small&amp;gt;10 µm recommended&amp;lt;/small&amp;gt;&lt;br /&gt;
  | 10–20 µm&lt;br /&gt;
  | 5 µm&lt;br /&gt;
  |-&lt;br /&gt;
  ! Storage&lt;br /&gt;
  | OCT, −80°C&lt;br /&gt;
  | −80°C&lt;br /&gt;
  | Room temp&lt;br /&gt;
  |-&lt;br /&gt;
  ! RNA quality&lt;br /&gt;
  | colspan=&amp;quot;3&amp;quot; | DV200 ≥ 30% recommended; higher scores improve sensitivity&lt;br /&gt;
  |-&lt;br /&gt;
  ! Histology&lt;br /&gt;
  | colspan=&amp;quot;3&amp;quot; | Sectioning &amp;amp; H&amp;amp;E performed by the [https://ki-sbc.mit.edu/histology Tang Histology Facility] (KI). Coordinate with BMC staff to arrange.&lt;br /&gt;
 |}&lt;br /&gt;
 &lt;br /&gt;
For current pricing, see [[BioMicroCenter:Pricing]] → Single Cell &amp;amp; Spatial Services → Visium. Sequencing is charged separately; 400–800M reads per tile recommended for Visium HD.&lt;br /&gt;
 &lt;br /&gt;
=== SUBMISSION ===&lt;br /&gt;
Submit via [https://mit.ilabsolutions.com/service_item/new/3381?spt_id=12903 iLabs (assisted service)]. Coordinate with BMC staff at least one week in advance. Tissue processing begins at the [https://ki-sbc.mit.edu/histology Tang Histology Facility]; please arrange sectioning separately.&lt;br /&gt;
 &lt;br /&gt;
|&lt;br /&gt;
 &lt;br /&gt;
=== 10X Visium HD ===&lt;br /&gt;
[[File:VisiumHD_loupe_example.jpg|thumb|right|350px|Example of FF data from Visium HD]]&lt;br /&gt;
 &lt;br /&gt;
&#039;&#039;&#039;FAQs for Users&#039;&#039;&#039;&amp;lt;BR&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;1) What tissues are compatible?&#039;&#039;&#039;&amp;lt;br&amp;gt;&lt;br /&gt;
Visium HD (probe-based) supports human and mouse tissues in FF, FxF, and FFPE formats. Visium HD 3&#039; (polyA) supports fresh frozen tissue from any species. 10x Genomics maintains a [https://www.10xgenomics.com/support/spatial-gene-expression-hd/documentation/steps/tissue-prep-for-fresh-frozen/visium-hd-spatial-gene-expression-tested-tissues-fresh-frozen list of tested tissues]. Additional optimization may be required for tissues with high fat content. Consult BMC staff before starting.&lt;br /&gt;
&amp;lt;br&amp;gt;&amp;lt;br&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;2) How do I prepare and submit tissue?&#039;&#039;&#039;&amp;lt;br&amp;gt;&lt;br /&gt;
Tissue sectioning and H&amp;amp;E staining are performed by the [https://ki-sbc.mit.edu/histology Hope Babette Tang (1983) Histology Facility] at the Koch Institute. Coordinate with both the Histology Facility and BMC staff before your first submission. FF tissue should be embedded in OCT and stored at −80°C; FFPE blocks at room temperature.&lt;br /&gt;
&amp;lt;br&amp;gt;&amp;lt;br&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;3) What is the resolution?&#039;&#039;&#039;&amp;lt;br&amp;gt;&lt;br /&gt;
Visium HD captures data at 2 µm bin resolution (single-cell scale). SpaceRanger aggregates into 8 µm and 16 µm bins for higher sensitivity by default; custom bin sizes are also supported. Cell segmentation based on H&amp;amp;E imaging is available in the Loupe Browser pipeline.&lt;br /&gt;
&amp;lt;br&amp;gt;&amp;lt;br&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;4) How much sequencing is needed?&#039;&#039;&#039;&amp;lt;br&amp;gt;&lt;br /&gt;
400–800M reads per tile is recommended for Visium HD. Sequencing is submitted separately via iLabs. Compatible platforms include NovaSeq SP, NovaSeqX, and Element AVITI. Discuss read depth with BMC staff based on your tissue type and goals.&lt;br /&gt;
&amp;lt;br&amp;gt;&amp;lt;br&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;5) Does Visium HD require permeabilization optimization?&#039;&#039;&#039;&amp;lt;br&amp;gt;&lt;br /&gt;
No. Visium HD does not require permeabilization time optimization. For fresh frozen tissues new to the platform, a test array is available to verify tissue quality and placement before committing a full experiment.&lt;br /&gt;
&amp;lt;br&amp;gt;&amp;lt;br&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;6) Can I detect proteins alongside gene expression?&#039;&#039;&#039;&amp;lt;br&amp;gt;&lt;br /&gt;
Yes. H&amp;amp;E or IF staining can be performed prior to CytAssist processing. Antibody panels for protein co-detection (human tissues, FFPE only) are available from 10x Genomics. Discuss your experimental design with BMC staff in advance.&lt;br /&gt;
&amp;lt;br&amp;gt;&amp;lt;br&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;7) What analysis outputs are provided?&#039;&#039;&#039;&amp;lt;br&amp;gt;&lt;br /&gt;
The BMC delivers FASTQ files, aligned BAM files, the spatial feature-barcode matrix (raw and filtered at 2, 8, and 16 µm bins), and a Loupe Browser file. Downstream informatics support is available through BMC staff.&lt;br /&gt;
&amp;lt;br&amp;gt;&amp;lt;br&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;8) Can I combine Visium HD with single-cell data?&#039;&#039;&#039;&amp;lt;br&amp;gt;&lt;br /&gt;
Yes. Visium HD data integrates with single-cell or single-nucleus RNA-seq (e.g., 10x Chromium Flex) for cell-type deconvolution. Starting from the same tissue block, nuclei can be isolated for Flex single-cell gene expression. Coordinate with BMC staff if planning a multi-modal experiment.&lt;br /&gt;
&amp;lt;br&amp;gt;&amp;lt;br&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;9) My gene of interest is exogenous (GFP, reporter, viral) or missing from the probe set. What are my options?&#039;&#039;&#039;&amp;lt;br&amp;gt;&lt;br /&gt;
The probe-based Visium HD assay does not capture exogenous sequences by default. Custom probes can be designed for detection — see 10x Genomics technical note CG000621. Additionally, a small fraction of endogenous genes are excluded from the standard probe sets due to predicted off-target activity: see the [https://www.10xgenomics.com/support/spatial-gene-expression-hd/documentation/steps/probe-sets/visium-human-transcriptome-probe-set-v2-1 human excluded gene list] (~2.1% of genes) and [https://www.10xgenomics.com/support/spatial-gene-expression-hd/documentation/steps/probe-sets/visium-mouse-transcriptome-probe-set-v2-0 mouse excluded gene list] (~1.8% of genes). Alternatively, the polyA-based Visium HD 3&#039; assay captures all polyadenylated transcripts and does not require custom probes.&lt;br /&gt;
&amp;lt;br&amp;gt;&lt;br /&gt;
 &lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
== AVITI24 CYTOPROFILING &amp;amp; IN SITU SEQUENCING ==&lt;br /&gt;
&amp;lt;div style=&amp;quot;background:#fff8e6; border-left:4px solid #c8960c; padding:8px 12px; margin-bottom:12px;&amp;quot;&amp;gt;&#039;&#039;&#039;Beta Service — Early Access:&#039;&#039;&#039; The AVITI24 Teton CytoProfiling and DISS services at the BioMicro Center are operational but not yet routine. &#039;&#039;&#039;Consultation with BMC staff is required before submitting samples.&#039;&#039;&#039;&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;!-- placeholder image left: AVITI24 instrument photo or Teton CytoProfiling schematic --&amp;gt;&lt;br /&gt;
&amp;lt;!-- replace with: [[File:AVITI24_instrument.png|thumb|left|400px]] --&amp;gt;&lt;br /&gt;
&lt;br /&gt;
The BioMicro Center provides access to the [https://www.elementbiosciences.com/products/aviti24 Element Biosciences AVITI24] platform for in situ single-cell multiomics. Using [https://www.elementbiosciences.com/products/aviti24/cytoprofiling Teton™ CytoProfiling], the instrument co-detects RNA transcripts, proteins (including phospho-proteins), and cell morphology at subcellular resolution — directly in intact cells, without library preparation. Targeted in situ sequencing via Direct In Sample Sequencing (DISS) can be run simultaneously in the same experiment. Because this is an early-access service, results and run parameters are less well-established than our established offerings. Consultation with BMC staff is strongly encouraged before designing experiments.&lt;br /&gt;
&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
&lt;br /&gt;
{|&lt;br /&gt;
 |- style=&amp;quot;vertical-align: top;&amp;quot;&lt;br /&gt;
 |style=&amp;quot;width: 500px;&amp;quot;|&lt;br /&gt;
&lt;br /&gt;
=== PLATFORM SPECIFICATIONS ===&lt;br /&gt;
 {| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
  ! Instrument&lt;br /&gt;
  | Element Biosciences AVITI24&lt;br /&gt;
  |-&lt;br /&gt;
  ! Technology&lt;br /&gt;
  | Teton™ CytoProfiling (ABC Sequencing)&lt;br /&gt;
  |-&lt;br /&gt;
  ! Flow cell formats&lt;br /&gt;
  | 1-well · 12-well (96-well format) · 48-well (384-well format)&lt;br /&gt;
  |-&lt;br /&gt;
  ! Imageable area&lt;br /&gt;
  | Up to 10 cm² per flow cell side&lt;br /&gt;
  |-&lt;br /&gt;
  ! Max cells per run&lt;br /&gt;
  | Up to 1 million adherent cells per flow cell&lt;br /&gt;
  |-&lt;br /&gt;
  ! Transcript density&lt;br /&gt;
  | ~100–200 transcripts per 100 µm²&lt;br /&gt;
 |}&lt;br /&gt;
&lt;br /&gt;
=== ASSAY CAPABILITIES ===&lt;br /&gt;
 {| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
  ! style=&amp;quot;width:18%&amp;quot;|&lt;br /&gt;
  ! style=&amp;quot;width:27%; background:#e8f0f8;&amp;quot;| Teton CytoProfiling&lt;br /&gt;
  ! style=&amp;quot;width:27%; background:#e8f4e8;&amp;quot;| DISS (low output) &amp;lt;BR&amp;gt;&amp;lt;small&amp;gt;&#039;&#039;&#039;available&#039;&#039;&#039;&amp;lt;/small&amp;gt;&lt;br /&gt;
  ! style=&amp;quot;width:27%; background:#f5f0f0;&amp;quot;| DISS (high output) &amp;lt;BR&amp;gt;&amp;lt;small&amp;gt;&#039;&#039;not yet released&#039;&#039;&amp;lt;/small&amp;gt;&lt;br /&gt;
  |-&lt;br /&gt;
  ! RNA&lt;br /&gt;
  | 350 transcripts &amp;lt;BR&amp;gt;&amp;lt;small&amp;gt;panel-based&amp;lt;/small&amp;gt;&lt;br /&gt;
  | Targeted genes &amp;lt;BR&amp;gt;&amp;lt;small&amp;gt;~50 nt, 1 priming event; CRISPR guides, barcodes, expressed mutations&amp;lt;/small&amp;gt;&lt;br /&gt;
  | &amp;lt;span style=&amp;quot;color:#999;&amp;quot;&amp;gt;Whole transcriptome &amp;lt;BR&amp;gt;&amp;lt;small&amp;gt;polyT prime; 100–300 nt&amp;lt;/small&amp;gt;&amp;lt;/span&amp;gt;&lt;br /&gt;
  |-&lt;br /&gt;
  ! Protein&lt;br /&gt;
  | colspan=&amp;quot;3&amp;quot; | ~18 targets &amp;amp;nbsp;&amp;lt;small&amp;gt;panels of 24; rabbit monoclonal primary; custom available&amp;lt;/small&amp;gt;&lt;br /&gt;
  |-&lt;br /&gt;
  ! Morphology&lt;br /&gt;
  | colspan=&amp;quot;3&amp;quot; | Nucleus, actin, membrane (all species) · ER, Golgi, mitochondria (human only)&lt;br /&gt;
  |-&lt;br /&gt;
  ! Panels / probes&lt;br /&gt;
  | MAPK Cell Cycle · MAPK Apoptosis · Immuno-Oncology · Neuroscience &amp;lt;BR&amp;gt;&amp;lt;small&amp;gt;mix up to 3 panels per run; consult staff for latest&amp;lt;/small&amp;gt;&lt;br /&gt;
  | User-designed probes&lt;br /&gt;
  | &amp;lt;span style=&amp;quot;color:#999;&amp;quot;&amp;gt;User-designed or polyT probe&amp;lt;/span&amp;gt;&lt;br /&gt;
 |}&lt;br /&gt;
Teton CytoProfiling and DISS (low output) can be run simultaneously in the same experiment. Custom antibody add-ons require unconjugated rabbit monoclonal primaries. Consult BMC staff for current options.&lt;br /&gt;
&lt;br /&gt;
=== SAMPLE REQUIREMENTS ===&lt;br /&gt;
 {| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
  ! Supported&lt;br /&gt;
  | Monolayer cell culture (adherent); pelleted cell suspension (spun down and resuspended)&lt;br /&gt;
  |-&lt;br /&gt;
  ! Not yet supported&lt;br /&gt;
  | Complex 3D culture, organoids, tissue sections — contact BMC to discuss future availability&lt;br /&gt;
  |-&lt;br /&gt;
  ! Cell density&lt;br /&gt;
  | Consult BMC staff; optimized per cell type&lt;br /&gt;
  |-&lt;br /&gt;
  ! Species&lt;br /&gt;
  | Human (all current fixed panels); other species — consult staff regarding panel compatibility&lt;br /&gt;
 |}&lt;br /&gt;
&lt;br /&gt;
=== SUBMISSION ===&lt;br /&gt;
&amp;lt;!-- iLabs link stub: replace once service item is created --&amp;gt;&lt;br /&gt;
&amp;lt;!-- [https://mit.ilabsolutions.com/service_item/new/XXXX iLabs (assisted service)] --&amp;gt;&lt;br /&gt;
Submission via iLabs is not yet available. Contact BMC staff directly to discuss your experiment before scheduling. &#039;&#039;&#039;Consultation is required for all AVITI24 Teton and DISS submissions.&#039;&#039;&#039;&lt;br /&gt;
&lt;br /&gt;
|&lt;br /&gt;
&lt;br /&gt;
=== AVITI24 Teton CytoProfiling at BMC ===&lt;br /&gt;
[[File:AVITI24_Boyer_cardiomyocytes.jpg|thumb|right|300px|Cytoprofiling of cardiomyocytes. Collaboration with the Boyer Lab.]]&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;About the Service&#039;&#039;&#039;&amp;lt;BR&amp;gt;&lt;br /&gt;
The AVITI24 at BMC supports Teton™ CytoProfiling — an in situ multiomics approach that co-detects RNA transcripts, proteins (including phospho-proteins), and cell morphology directly inside intact cells on the flow cell. Each run cycles through Cell Painting (morphology imaging), antibody scoring, and in situ sequencing rounds without any library preparation. A full run takes 24–48 hours end-to-end with approximately 45 minutes of hands-on time, capturing ~100–200 transcripts per 100 µm² at subcellular resolution.&lt;br /&gt;
&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
The platform is designed for cells in monolayer culture or spun-down suspension. It profiles up to 350 RNA targets, ~18 protein targets, and 6 morphology channels simultaneously in a single experiment — giving a co-registered multiomic readout per cell that would otherwise require separate assays on separate instruments.&lt;br /&gt;
&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
Targeted in situ sequencing (DISS, low output) can be layered onto any Teton run. This uses a user-designed probe (~50 nt, 1 priming event) to detect specific sequences in situ — useful for CRISPR guide detection, barcode readout, or expressed mutation calling. Note that reads are too short for TCR/BCR sequencing; consult BMC staff if your application involves immune repertoire.&lt;br /&gt;
&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;Available Assays&#039;&#039;&#039;&amp;lt;BR&amp;gt;&lt;br /&gt;
BMC supports all four currently available fixed Teton panels (MAPK Cell Cycle, MAPK Apoptosis, Immuno-Oncology, Neuroscience). Up to three panels can be combined per run, giving up to 350 RNA targets and ~18 usable protein targets in a single experiment. Custom antibody add-ons are possible using unconjugated rabbit monoclonal primary antibodies — no conjugation or barcoding required. Discuss panel selection with BMC staff; reagent availability and lead time vary.&lt;br /&gt;
&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;Data Outputs&#039;&#039;&#039;&amp;lt;BR&amp;gt;&lt;br /&gt;
The BMC provides core analysis outputs including:&lt;br /&gt;
* Cell-by-gene expression matrix (350 transcript targets per panel)&lt;br /&gt;
* Cell-by-protein parameter data (~18 antibody targets)&lt;br /&gt;
* Morphology feature data per cell (nucleus, actin, membrane; ER, Golgi, mitochondria for human)&lt;br /&gt;
* Spatial maps with morphology channel overlays&lt;br /&gt;
* [https://www.elementbiosciences.com/products/elembio-catalyst ElemBio Catalyst / CytoCanvas]-compatible files for interactive visualization and downstream analysis&lt;br /&gt;
&amp;lt;BR&amp;gt;&lt;br /&gt;
Additional informatics support is available through BMC staff.&lt;br /&gt;
&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;Pricing&#039;&#039;&#039;&amp;lt;BR&amp;gt;&lt;br /&gt;
See [[BioMicroCenter:Pricing|BioMicroCenter:Pricing]] → Single Cell &amp;amp; Spatial Services → AVITI24 for current pricing. Sequencing-only runs on the AVITI24 are charged at standard AVITI24 sequencing rates.&lt;br /&gt;
&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;Service Status&#039;&#039;&#039;&amp;lt;BR&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;background:#fff8e6; border-left:4px solid #c8960c; padding:8px 12px; margin-top:8px;&amp;quot;&amp;gt;This service is in &#039;&#039;&#039;beta&#039;&#039;&#039;. The instrument is operational and BMC has completed initial runs, but experimental parameters are still being validated. We welcome early users willing to work collaboratively with BMC staff to establish protocols.&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
|}&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:Pricing&amp;diff=131222</id>
		<title>BioMicroCenter:Pricing</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:Pricing&amp;diff=131222"/>
		<updated>2026-03-24T19:29:06Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: Add AVITI24 cytoprofiling/DISS pricing under Single Cell &amp;amp; Spatial Services&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;{{BioMicroCenter}}&lt;br /&gt;
&lt;br /&gt;
== SEQUENCING - HIGH OUTPUT ==&lt;br /&gt;
Please note that samples submitted from [[BioMicroCenter:CoreDeps|&#039;&#039;&#039;CORE LAB&#039;&#039;&#039;]]s will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason. &amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
&lt;br /&gt;
=== NOVASEQX / NOVASEQ6000 SEQUENCING ===&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; border=1 &lt;br /&gt;
 ! NOVASEQ SEQUENCING&lt;br /&gt;
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]&lt;br /&gt;
 !width=80| MIT&lt;br /&gt;
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]&lt;br /&gt;
 !width=80| unit&lt;br /&gt;
 ! Notes&lt;br /&gt;
 |-&lt;br /&gt;
 |NovaSeq SP 100nt&lt;br /&gt;
 |align=right| $3,699&lt;br /&gt;
 |align=right| $4,068&lt;br /&gt;
 |align=right| $5,178&lt;br /&gt;
 |rowspan=9| Per flowcell&lt;br /&gt;
 |rowspan=9| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year&lt;br /&gt;
 |-&lt;br /&gt;
 |NovaSeq SP 300nt** &lt;br /&gt;
 |align=right| $5,238&lt;br /&gt;
 |align=right| $5,761&lt;br /&gt;
 |align=right| $7,333.20&lt;br /&gt;
 |-&lt;br /&gt;
 |NovaSeq SP 500nt&lt;br /&gt;
 |align=right| $7,020&lt;br /&gt;
 |align=right| $7,722&lt;br /&gt;
 |align=right| $9,828&lt;br /&gt;
 |-&lt;br /&gt;
 |NovaSeq S1 100nt**&lt;br /&gt;
 |align=right| $5,832&lt;br /&gt;
 |align=right| $6,415.20&lt;br /&gt;
 |align=right| $8,164.80&lt;br /&gt;
 |-&lt;br /&gt;
 |NovaSeq S1 200nt**&lt;br /&gt;
 |align=right| $7,452&lt;br /&gt;
 |align=right| $8,197.20&lt;br /&gt;
 |align=right| $10,432&lt;br /&gt;
 |-&lt;br /&gt;
 |NovaSeq S1 300nt**&lt;br /&gt;
 |align=right| $8,127&lt;br /&gt;
 |align=right| $8,939.70&lt;br /&gt;
 |align=right| $11,377.80&lt;br /&gt;
 |-&lt;br /&gt;
 |NovaSeq S2 100nt**&lt;br /&gt;
 |align=right| $10,395&lt;br /&gt;
 |align=right| $11,434.50&lt;br /&gt;
 |align=right| $14,553&lt;br /&gt;
 |-&lt;br /&gt;
 |NovaSeq S2 200nt**&lt;br /&gt;
 |align=right| $12,960&lt;br /&gt;
 |align=right| $14,256&lt;br /&gt;
 |align=right| $18,144&lt;br /&gt;
 |-&lt;br /&gt;
 |NovaSeq S2 300nt**&lt;br /&gt;
 |align=right| $13,500&lt;br /&gt;
 |align=right| $14,850&lt;br /&gt;
 |align=right| $18,900&lt;br /&gt;
 |-&lt;br /&gt;
 |NovaSeq S4 50nt &lt;br /&gt;
 |align=right| $3,780&lt;br /&gt;
 |align=right| $4,158&lt;br /&gt;
 |align=right| $5,292&lt;br /&gt;
 |rowspan=2| Per Lane&lt;br /&gt;
 |rowspan=2| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year&lt;br /&gt;
 |-&lt;br /&gt;
 |NovaSeq S4 300nt&lt;br /&gt;
 |align=right| $5,400&lt;br /&gt;
 |align=right| $5,940&lt;br /&gt;
 |align=right| $7,560&lt;br /&gt;
 |-&lt;br /&gt;
 |NovaSeqX 10b 300nt LANE^^&lt;br /&gt;
 |colspan=3| pricing in ilabs&lt;br /&gt;
 |rowspan=2| Per Lane&lt;br /&gt;
 |rowspan=3| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year&lt;br /&gt;
 |-&lt;br /&gt;
 |NovaSeqX 25b 300nt LANE^^&lt;br /&gt;
 |colspan=3| pricing in ilabs&lt;br /&gt;
 |-&lt;br /&gt;
 |NovaSeqX 25b 100nt Flowcell^^&lt;br /&gt;
 |colspan=3| pricing in ilabs&lt;br /&gt;
 | Per Flowcell&lt;br /&gt;
 |-&lt;br /&gt;
|}&lt;br /&gt;
 ** - minimal inventory maintained&lt;br /&gt;
 ^^ - through collaboration with nearby academic shared resources&lt;br /&gt;
&lt;br /&gt;
== SEQUENCING - MID OUTPUT ==&lt;br /&gt;
Please note that samples submitted from [[BioMicroCenter:CoreDeps|&#039;&#039;&#039;CORE LAB&#039;&#039;&#039;]]s will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason. &amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; border=1 &lt;br /&gt;
 ! ELEMENT AVITI SEQUENCING&lt;br /&gt;
 !width=80| Lane/Flowcell&lt;br /&gt;
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]&lt;br /&gt;
 !width=80| MIT&lt;br /&gt;
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]&lt;br /&gt;
 !width=80| unit&lt;br /&gt;
 ! Notes&lt;br /&gt;
 |-&lt;br /&gt;
 |rowspan=2| 75 PE&lt;br /&gt;
 | Full flowcell (2 lane, 800m read)&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1,900&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $2,090&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $2,660&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;| per flowcell&lt;br /&gt;
 |rowspan=5| Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year&lt;br /&gt;
 |-&lt;br /&gt;
 |One lane (~400m read)&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1,000&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1,100&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1,400&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;| per lane&lt;br /&gt;
 |-&lt;br /&gt;
 |rowspan=2| 150 PE&lt;br /&gt;
 | Full flowcell (2 lane, ~800m read)&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $2,650&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $2,915&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $3,710&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;| per flowcell&lt;br /&gt;
 |-&lt;br /&gt;
 |One lane (~400m read)&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1,375&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1,513&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1,925&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;| per lane&lt;br /&gt;
 |-&lt;br /&gt;
 | 300 PE&lt;br /&gt;
 | Full flowcell (~200m reads)&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $3,500&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $3,850&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $4,900&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;| per lane&lt;br /&gt;
|}&lt;br /&gt;
&amp;lt;!-- === NEXTSEQ500 SEQUENCING ===&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; border=1 &lt;br /&gt;
 ! NEXTSEQ SEQUENCING&lt;br /&gt;
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]&lt;br /&gt;
 !width=80| MIT&lt;br /&gt;
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]&lt;br /&gt;
 !width=80| unit&lt;br /&gt;
 ! Notes&lt;br /&gt;
 |-&lt;br /&gt;
 |91nt kit &amp;lt;BR&amp;gt; (eg. 40PE or 75SE)&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $2,570&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $2,827&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $3,598&lt;br /&gt;
 |align=&amp;quot;center&amp;quot; rowspan=&amp;quot;3&amp;quot;| per flowcell&lt;br /&gt;
 |rowspan=&amp;quot;3&amp;quot;| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year &lt;br /&gt;
 |-&lt;br /&gt;
 |166nt kit &amp;lt;BR&amp;gt; (eg. 80PE or 150SE)&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $4,375&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $4,812.50&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $6,125&lt;br /&gt;
 |-&lt;br /&gt;
 |316nt kit &amp;lt;BR&amp;gt; (eg. 150PE or 300SE)&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $6,420&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $7,062&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $8,988&lt;br /&gt;
|}&lt;br /&gt;
--&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== SEQUENCING - LOW OUTPUT ==&lt;br /&gt;
Please note that samples submitted from [[BioMicroCenter:CoreDeps|&#039;&#039;&#039;CORE LAB&#039;&#039;&#039;]]s will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason. &amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; border=1 &lt;br /&gt;
 ! SINGULAR G4 SEQUENCING&lt;br /&gt;
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]&lt;br /&gt;
 !width=80| MIT&lt;br /&gt;
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]&lt;br /&gt;
 !width=80| unit&lt;br /&gt;
 ! Notes&lt;br /&gt;
 |-&lt;br /&gt;
 |50 PE: F3 Lane (~100m reads)&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $300&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $330&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $420&lt;br /&gt;
 |align=&amp;quot;center&amp;quot; | per lane&lt;br /&gt;
 |rowspan=3| Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year&lt;br /&gt;
 |-&lt;br /&gt;
 |Rapid 100nt: F3 flowcell (~400m reads)*&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1200&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1321&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1680&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;| per flowcell&lt;br /&gt;
 |-&lt;br /&gt;
 |150 PE: F3 Lane (~100m reads)&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $450&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $495&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $630&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;| per lane&lt;br /&gt;
&lt;br /&gt;
|}&lt;br /&gt;
 * Rapid flowcells are required for adapted Illumina libraries.&lt;br /&gt;
&lt;br /&gt;
=== MISEQ SEQUENCING ===&lt;br /&gt;
==== MiSeq i100 Assisted ====&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; border=1 &lt;br /&gt;
 !colspan=&amp;quot;2&amp;quot;| MISEQ i100 SEQUENCING&lt;br /&gt;
 !rowspan=2 width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]&lt;br /&gt;
 !rowspan=2 width=80| MIT&lt;br /&gt;
 !rowspan=2 width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]&lt;br /&gt;
 !rowspan=2 width=80| unit&lt;br /&gt;
 !rowspan=2| Notes&lt;br /&gt;
 |-&lt;br /&gt;
 !Length&lt;br /&gt;
 !Reads&lt;br /&gt;
 |-&lt;br /&gt;
 |300nt&lt;br /&gt;
 |5M&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $715&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $786.5&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1001&lt;br /&gt;
 |align=&amp;quot;center&amp;quot; rowspan=9| per flowcell&lt;br /&gt;
 |rowspan=9| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year&lt;br /&gt;
 |-&lt;br /&gt;
 |600nt&lt;br /&gt;
 |5M&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1040&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1144&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1456&lt;br /&gt;
 |-&lt;br /&gt;
 |100nt&lt;br /&gt;
 |25M&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $925&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1017.5&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1295&lt;br /&gt;
 |-&lt;br /&gt;
 |300nt&lt;br /&gt;
 |25M&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1334&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1467.4&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1867.6&lt;br /&gt;
 |-&lt;br /&gt;
 |600nt&lt;br /&gt;
 |25M&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1570&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1727&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $2198&lt;br /&gt;
 |-&lt;br /&gt;
 |&amp;lt;b&amp;gt;1000&amp;lt;/b&amp;gt;nt&lt;br /&gt;
 |25M&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $2590&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $2849&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $3626&lt;br /&gt;
 |-&lt;br /&gt;
 |100nt&lt;br /&gt;
 |100M&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1540&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1694&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $2156&lt;br /&gt;
 |-&lt;br /&gt;
 |300nt&lt;br /&gt;
 |100M&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $2125&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $2337.5&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $2975&lt;br /&gt;
 |-&lt;br /&gt;
 |600nt&lt;br /&gt;
 |50M&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $2125&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $2337.5&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $2975&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
==== MiSeq i100 Walkup ====&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; border=1 &lt;br /&gt;
 !colspan=&amp;quot;2&amp;quot;| MISEQ i100 SEQUENCING&lt;br /&gt;
 !rowspan=2 width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]&lt;br /&gt;
 !rowspan=2 width=80| MIT&lt;br /&gt;
 !rowspan=2 width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]&lt;br /&gt;
 !rowspan=2 width=80| unit&lt;br /&gt;
 !rowspan=2 width=80| RunTime&lt;br /&gt;
 !rowspan=2| Notes&lt;br /&gt;
 |-&lt;br /&gt;
 !Length&lt;br /&gt;
 !Reads&lt;br /&gt;
 |-&lt;br /&gt;
 |colspan=2 | Machine usage&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $120&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $132&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| NA&lt;br /&gt;
 |&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;| per flowcell&lt;br /&gt;
 | rowspan=10| training and lab storage on BMC servers required. &lt;br /&gt;
 |-&lt;br /&gt;
 |300nt&lt;br /&gt;
 |5M&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $381&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $419.1&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| NA&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;| 8h&lt;br /&gt;
 |align=&amp;quot;center&amp;quot; rowspan=9| per kit&lt;br /&gt;
 |-&lt;br /&gt;
 |600nt&lt;br /&gt;
 |5M&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $665&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $731.5&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| NA&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;| 16h&lt;br /&gt;
 |-&lt;br /&gt;
 |100nt&lt;br /&gt;
 |25M&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $565&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $621.5&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| NA&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;| 5h&lt;br /&gt;
 |-&lt;br /&gt;
 |300nt&lt;br /&gt;
 |25M&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $920&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1012&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| NA&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;| 8h&lt;br /&gt;
 |-&lt;br /&gt;
 |600nt&lt;br /&gt;
 |25M&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1125&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1238&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| NA&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;| 16h&lt;br /&gt;
 |-&lt;br /&gt;
 |1000nt&lt;br /&gt;
 |25M&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1921&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $2113.1&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| NA&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;| 24h&lt;br /&gt;
 |-&lt;br /&gt;
 |100nt&lt;br /&gt;
 |100M&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1130&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1243&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| NA&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;| 5h&lt;br /&gt;
 |-&lt;br /&gt;
 |300nt&lt;br /&gt;
 |100M&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1638.5&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1802.35&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| NA&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;| 8h&lt;br /&gt;
 |-&lt;br /&gt;
 |600nt&lt;br /&gt;
 |50M&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1638.5&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1802.35&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| NA&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;| 16h&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
==== MiSeq classic ====&lt;br /&gt;
* MiSeq is off contract and will be retired upon instrument failure &lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; border=1 &lt;br /&gt;
 ! MISEQ SEQUENCING&lt;br /&gt;
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]&lt;br /&gt;
 !width=80| MIT&lt;br /&gt;
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]&lt;br /&gt;
 !width=80| unit&lt;br /&gt;
 ! Notes&lt;br /&gt;
 |-&lt;br /&gt;
 |70nt V2 kit &amp;lt;BR&amp;gt; (eg. 30PE or 70SE)&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1,320&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1,452&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1,848&lt;br /&gt;
 |align=&amp;quot;center&amp;quot; rowspan=6| per lane&lt;br /&gt;
 |rowspan=6| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year&lt;br /&gt;
 |-&lt;br /&gt;
 |150nt V3 kits &amp;lt;BR&amp;gt; (eg. 75PE)&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1,430&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1,573&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $2,002&lt;br /&gt;
 |-&lt;br /&gt;
 |300nt V2 kit&amp;lt;BR&amp;gt; (eg. 150PE)&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1,595&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1,754.50&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $2,233&lt;br /&gt;
 |-&lt;br /&gt;
 |500nt V2 kit &amp;lt;BR&amp;gt; (eg. 250PE)&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1,760&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1,936&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $2,464&lt;br /&gt;
 |-&lt;br /&gt;
 |600nt V3 kit&amp;lt;BR&amp;gt; (eg. 300PE)&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $2,200&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $2,420&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $3,080&lt;br /&gt;
 |-&lt;br /&gt;
 |500nt &#039;&#039;&#039;NANO&#039;&#039;&#039; Run &amp;lt;BR&amp;gt; (eg. 250PE)&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $770&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $847&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1,078&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
=== OTHER SHORT READ SERVICES ===&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; border=1 &lt;br /&gt;
 ! SHORT READ SUPPORTING SERVICES&lt;br /&gt;
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]&lt;br /&gt;
 !width=80| MIT&lt;br /&gt;
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]&lt;br /&gt;
 !width=80| unit&lt;br /&gt;
 ! Notes&lt;br /&gt;
 |-&lt;br /&gt;
 | Quality Control Only -only one QC is included per sequencing lane.&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $35&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $38.50&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $49&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;| per sample&lt;br /&gt;
 | Includes RT-PCR and Frag.Analyzer&lt;br /&gt;
 |-&lt;br /&gt;
 | Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling.&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $15&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $16.50&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $21&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;| per sample&lt;br /&gt;
 | Includes Fluorometric quantification and Frag.Analyzer&lt;br /&gt;
 |-&lt;br /&gt;
 |qPCR only (short read)&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $25&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $27.50&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $35&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;| per sample&lt;br /&gt;
 | Includes qPCR values from standard QC only&lt;br /&gt;
 |-&lt;br /&gt;
 |qPCR only - full plate(short read)&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $400&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $440&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $560&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;| per sample&lt;br /&gt;
 | excludes column 1 (used for controls)&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
== LONG READ SEQUENCING ==&lt;br /&gt;
&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; border=1 &lt;br /&gt;
 ! PROMETHION SEQUENCING&lt;br /&gt;
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]&lt;br /&gt;
 !width=80| MIT&lt;br /&gt;
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]&lt;br /&gt;
 !width=80| unit&lt;br /&gt;
 ! Notes&lt;br /&gt;
 |-&lt;br /&gt;
 | 72 hour Flowcell &lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1,200&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1,320&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1,680&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;|per Flowcell&lt;br /&gt;
 ||Fastq stored for 1y. Trace data stored only 30d.&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; border=1 &lt;br /&gt;
 ! PACBIO REVIO SEQUENCING^^&lt;br /&gt;
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]&lt;br /&gt;
 !width=80| MIT&lt;br /&gt;
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]&lt;br /&gt;
 !width=80| unit&lt;br /&gt;
 ! Notes&lt;br /&gt;
 |-&lt;br /&gt;
 | 72 hour Flowcell &lt;br /&gt;
 |colspan=3 | pricing in ilabs&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;|per Flowcell&lt;br /&gt;
 ||Fastq stored for 1y. Trace data stored only 30d.&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
 ^^ - through collaboration with nearby academic shared resources&lt;br /&gt;
&lt;br /&gt;
== LIBRARY GENERATION ==&lt;br /&gt;
=== SHORT READ LIBRARY - CONVERSION ===&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
 !width=100| Input library&lt;br /&gt;
 !width=100| Convert To&lt;br /&gt;
 !width=100| Method&lt;br /&gt;
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]&lt;br /&gt;
 !width=80| MIT&lt;br /&gt;
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]&lt;br /&gt;
 ! Notes&lt;br /&gt;
 |-&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;|Illumina&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;|Singular&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;|Index Overwrite (per sample)&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $30&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $33&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $42&lt;br /&gt;
 | Includes QC and reindexing&lt;br /&gt;
 |-&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;|Illumina&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;|Singular&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;|P5/P7 conversion (per pool)&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $100&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $110&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $140&lt;br /&gt;
 | Includes QC. Samples not reindexed &amp;lt;BR&amp;gt;&amp;lt;B&amp;gt; Requires full flowcell for sequencing (Rapid) &amp;lt;/B&amp;gt;&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
=== SHORT READ LIBRARY - DNA ===&lt;br /&gt;
&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
 !width=160| ILLUMINA Sample Preparation&lt;br /&gt;
 !width=60| Type&lt;br /&gt;
 !width=60| Batch Size&lt;br /&gt;
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]&lt;br /&gt;
 !width=80| MIT&lt;br /&gt;
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]&lt;br /&gt;
 ! Notes&lt;br /&gt;
 |-&lt;br /&gt;
 ! colspan=&amp;quot;4&amp;quot; | &#039;&#039;&#039;STANDARD THROUGHPUT DNA&#039;&#039;&#039; &amp;lt;BR&amp;gt; Prices for individual samples. Repreps will typically be attempted on failed samples without charge.&lt;br /&gt;
 ! colspan=&amp;quot;3&amp;quot; style=&amp;quot;color: blue;&amp;quot; | &#039;&#039;&#039;HIGH THROUGHPUT DNA&#039;&#039;&#039; &amp;lt;BR&amp;gt; Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (&amp;lt;=5ul) submission must be coordinated with BMC staff. Initial QC not included.&lt;br /&gt;
 |-&lt;br /&gt;
 |rowspan=3 |LIGATION BASED&amp;lt;BR&amp;gt;NEB UltraII&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;|STD&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;| per sample&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $105&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $115&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $157&lt;br /&gt;
 | Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).&lt;br /&gt;
 |- style=&amp;quot;color: blue;&amp;quot;&lt;br /&gt;
 |align=&amp;quot;center&amp;quot; rowspan=2|HTL&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;| 24&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $900&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $990&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1,260&lt;br /&gt;
 |rowspan=2 | Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).&lt;br /&gt;
 |- style=&amp;quot;color: blue;&amp;quot;&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;| 96&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1,800&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1,980&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $2,520&lt;br /&gt;
 |-&lt;br /&gt;
&lt;br /&gt;
 |Tagmentation &amp;lt;BR&amp;gt; NEXTERA-XT&lt;br /&gt;
 |align=&amp;quot;center&amp;quot; rowspan=2|STD&lt;br /&gt;
 |align=&amp;quot;center&amp;quot; rowspan=2|per Sample&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $105&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $115&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $157&lt;br /&gt;
 | rowspan=&amp;quot;2&amp;quot; | Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA). &lt;br /&gt;
 |-&lt;br /&gt;
 |Tagmentation &amp;lt;BR&amp;gt; NEXTERA-FLEX&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $157&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $173&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $236&lt;br /&gt;
 &lt;br /&gt;
 |- style=&amp;quot;color: blue;&amp;quot;&lt;br /&gt;
 |rowspan=2 | NEXTERA-XT OR FLEX&lt;br /&gt;
 |rowspan=2 align=&amp;quot;center&amp;quot;| HTL&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;| &#039;&#039;24&#039;&#039;&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $900&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $990&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $1,260&lt;br /&gt;
 |rowspan=2 | Includes tagmentation, indexing, enrichment, and quality control spotcheck(SYBR and FA).&lt;br /&gt;
 |-&lt;br /&gt;
 |align=&amp;quot;center&amp;quot; style=&amp;quot;color: blue;&amp;quot; | &#039;&#039;96&#039;&#039;&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $1,800&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $1,980&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $2,520&lt;br /&gt;
 &lt;br /&gt;
 |-style=&amp;quot;color: blue;&amp;quot;&lt;br /&gt;
 |rowspan=&amp;quot;2&amp;quot;  | 16S Amplicon&lt;br /&gt;
 |align=&amp;quot;center&amp;quot; rowspan=2 | HTL&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;|48&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; | $1,900&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; | $2,090&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; | $2,660&lt;br /&gt;
 |rowspan=&amp;quot;2&amp;quot;   | Includes normalization, enrichment, pooling and quality control (SYBR and FA). &lt;br /&gt;
 |-style=&amp;quot;color: blue;&amp;quot;&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;|add&#039;l 48&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; | $650&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; | $715&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; | $910&lt;br /&gt;
&lt;br /&gt;
 |-style=&amp;quot;color: blue;&amp;quot;&lt;br /&gt;
 |rowspan=&amp;quot;2&amp;quot;   | Other Amplicon&lt;br /&gt;
 |rowspan=2 align=&amp;quot;center&amp;quot;| HTL&lt;br /&gt;
 |align=&amp;quot;center&amp;quot; |48&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; | $1,600&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; | $1,760&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; | $2,240&lt;br /&gt;
 |rowspan=&amp;quot;2&amp;quot;   | Includes normalization, enrichment, pooling and quality control (SYBR and FA). &amp;lt;BR&amp;gt; Samples *must* be submitted amplified with appropriate linkers.&lt;br /&gt;
 |- style=&amp;quot;color: blue;&amp;quot; &lt;br /&gt;
 |align=&amp;quot;center&amp;quot;|add&#039;l 48&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; | $630&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; | $693&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; | $945&lt;br /&gt;
 |-&lt;br /&gt;
 ! colspan=&amp;quot;7&amp;quot; | &#039;&#039;&#039;ADDITIONAL FEES&#039;&#039;&#039; &amp;lt;BR&amp;gt; Samples not in proper input format will be subject to these additional charges.&lt;br /&gt;
 |-&lt;br /&gt;
 |style=&amp;quot;color: blue;&amp;quot; colspan=2| High Throughput Setup&lt;br /&gt;
 |align=&amp;quot;center&amp;quot; style=&amp;quot;color: blue;&amp;quot; |48 samples &lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $60&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $66&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $84&lt;br /&gt;
 | &lt;br /&gt;
 |-&lt;br /&gt;
 |colspan=2|Sample Cleanup&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;|per sample&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $5&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $5.50&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $7&lt;br /&gt;
 |SPRI cleanup of samples to remove impurities. &lt;br /&gt;
 |-&lt;br /&gt;
 |style=&amp;quot;color: blue;&amp;quot; colspan=2|Quantification&lt;br /&gt;
 |align=&amp;quot;center&amp;quot; style=&amp;quot;color: blue;&amp;quot; |per sample&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $10&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $11&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $14&lt;br /&gt;
 |Quantification of samples (FA) prior to arraying.&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
=== SHORT READ LIBRARY - RNA ===&lt;br /&gt;
&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
 !width=160| ILLUMINA Sample Preparation&lt;br /&gt;
 !width=60| Type&lt;br /&gt;
 !width=60| Batch Size&lt;br /&gt;
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]&lt;br /&gt;
 !width=80| MIT&lt;br /&gt;
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]&lt;br /&gt;
 ! Notes&lt;br /&gt;
 |-&lt;br /&gt;
 ! colspan=&amp;quot;4&amp;quot; | &#039;&#039;&#039;STANDARD RNA&#039;&#039;&#039; &amp;lt;BR&amp;gt; Prices for individual samples. Repreps will typically be attempted on failed samples without charge.&lt;br /&gt;
 ! colspan=&amp;quot;3&amp;quot; style=&amp;quot;color: blue;&amp;quot; | &#039;&#039;&#039;HIGH THROUGHPUT RNA&#039;&#039;&#039; &amp;lt;BR&amp;gt; Repreps will NOT be attempted on failed samples without charge. Low volume (&amp;lt;=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.&lt;br /&gt;
 |-&lt;br /&gt;
 |rowspan=&amp;quot;3&amp;quot;|polyA based isolation &amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt; NEB UltraII RNAseq&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;|&amp;lt;B&amp;gt;STD&amp;lt;/B&amp;gt;&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;| per sample&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $157&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $173&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $236&lt;br /&gt;
 |Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).&lt;br /&gt;
 |-&lt;br /&gt;
 |rowspan=2 align=&amp;quot;center&amp;quot; style=&amp;quot;color: blue;&amp;quot;| &amp;lt;B&amp;gt;HTL&amp;lt;/b&amp;gt;&amp;lt;BR&amp;gt;(NEB only)&lt;br /&gt;
 |align=&amp;quot;center&amp;quot; style=&amp;quot;color: blue;&amp;quot; | &#039;&#039;24&#039;&#039;&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $1,500&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $1,650&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $2,100&lt;br /&gt;
 |Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).&lt;br /&gt;
 |-&lt;br /&gt;
 |align=&amp;quot;center&amp;quot; style=&amp;quot;color: blue;&amp;quot; | &#039;&#039;96&#039;&#039;&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $3,000&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $3,300&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $4,200&lt;br /&gt;
 |Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).&lt;br /&gt;
 |-&lt;br /&gt;
 &lt;br /&gt;
 |rowspan=&amp;quot;2&amp;quot;| High Throughput 3&#039; Digital Gene Expression (HT3DGE)&lt;br /&gt;
 |rowspan=2  align=&amp;quot;center&amp;quot; style=&amp;quot;color: blue;&amp;quot;| &amp;lt;B&amp;gt;HTL&amp;lt;/b&amp;gt;&lt;br /&gt;
 |align=&amp;quot;center&amp;quot; style=&amp;quot;color: blue;&amp;quot;| &#039;&#039;24&#039;&#039; &lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $1,050&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $1,155&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $1,470&lt;br /&gt;
 | rowspan=&amp;quot;2&amp;quot;  style=&amp;quot;color: blue;&amp;quot; | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.&lt;br /&gt;
 |-&lt;br /&gt;
 |align=&amp;quot;center&amp;quot; style=&amp;quot;color: blue;&amp;quot; | &#039;&#039;96&#039;&#039;&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $2,100&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $2,310&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $2,940&lt;br /&gt;
 |-&lt;br /&gt;
 &lt;br /&gt;
 |rowspan=&amp;quot;3&amp;quot;|Total RNA sequencing &amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt; NEB RNAseq +Ribodepletion&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;|&amp;lt;B&amp;gt;STD&amp;lt;/B&amp;gt;&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;| per sample&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $235&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $258.5&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $352.5&lt;br /&gt;
 |Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control. &lt;br /&gt;
 |-&lt;br /&gt;
 |rowspan=2 align=&amp;quot;center&amp;quot; style=&amp;quot;color: blue;&amp;quot;| &amp;lt;B&amp;gt;HTL&amp;lt;/b&amp;gt;&amp;lt;BR&amp;gt;(NEB only, covaris RNA fragmentation)&lt;br /&gt;
 |align=&amp;quot;center&amp;quot; style=&amp;quot;color: blue;&amp;quot; | &#039;&#039;24&#039;&#039;&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $2,500&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $2,750&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $3,500&lt;br /&gt;
 |rowspan=2|Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).&lt;br /&gt;
 |-&lt;br /&gt;
 |align=&amp;quot;center&amp;quot; style=&amp;quot;color: blue;&amp;quot; | &#039;&#039;96&#039;&#039;&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $5,000&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $5,500&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $7,000&lt;br /&gt;
 |-&lt;br /&gt;
 &lt;br /&gt;
 |rowspan=3|Low Input polyA+ &amp;lt;BR&amp;gt; Clontech SMARTseq + NexteraXT or Flex&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;| &#039;&#039;&#039;STD&#039;&#039;&#039;&amp;lt;BR&amp;gt;v4&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;|per sample&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $262&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $289&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $394&lt;br /&gt;
 | rowspan=&amp;quot;3&amp;quot; | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.&lt;br /&gt;
 |-&lt;br /&gt;
 |rowspan=&amp;quot;2&amp;quot; align=&amp;quot;center&amp;quot; style=&amp;quot;color: blue;&amp;quot;| &#039;&#039;&#039;HTL&#039;&#039;&#039;&amp;lt;br&amp;gt;v2&lt;br /&gt;
 |align=&amp;quot;center&amp;quot; style=&amp;quot;color: blue;&amp;quot; | &#039;&#039;24&#039;&#039;&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $2,000&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $2,200&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $2,800&lt;br /&gt;
 |-&lt;br /&gt;
 |align=&amp;quot;center&amp;quot; style=&amp;quot;color: blue;&amp;quot; | &#039;&#039;96&#039;&#039;&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $3,200&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $3,520&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $4,480&lt;br /&gt;
 |-&lt;br /&gt;
 &lt;br /&gt;
 |rowspan=&amp;quot;3&amp;quot;|Low Input Mammalian Total &amp;lt;BR&amp;gt; Clontech ZapR Kit&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;| STD&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;| per sample&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $210&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $231&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $315&lt;br /&gt;
 | rowspan=&amp;quot;1&amp;quot; | Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA). &lt;br /&gt;
 |-&lt;br /&gt;
 |rowspan=2 align=&amp;quot;center&amp;quot; style=&amp;quot;color: blue;&amp;quot;| &amp;lt;B&amp;gt;HTL&amp;lt;/b&amp;gt;&amp;lt;BR&amp;gt;(covaris RNA fragmentation)&lt;br /&gt;
 |align=&amp;quot;center&amp;quot; style=&amp;quot;color: blue;&amp;quot; | &#039;&#039;24&#039;&#039;&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $3000&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $3300&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $4200&lt;br /&gt;
 |rowspan=2|Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).&lt;br /&gt;
 |-&lt;br /&gt;
 |align=&amp;quot;center&amp;quot; style=&amp;quot;color: blue;&amp;quot; | &#039;&#039;96&#039;&#039;&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $5000&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $5500&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $7000&lt;br /&gt;
 |-&lt;br /&gt;
 | Small RNA&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;| STD&lt;br /&gt;
 | align=&amp;quot;center&amp;quot;|per sample&lt;br /&gt;
 | align=&amp;quot;right&amp;quot;|$289&lt;br /&gt;
 | align=&amp;quot;right&amp;quot;|$318&lt;br /&gt;
 | align=&amp;quot;right&amp;quot;|$433&lt;br /&gt;
 | Uses NEB, BIOO or QIAGEN small RNA kit&lt;br /&gt;
 |-&lt;br /&gt;
 ! colspan=&amp;quot;7&amp;quot; | &#039;&#039;&#039;ADDITIONAL FEES&#039;&#039;&#039; &amp;lt;BR&amp;gt; Samples not in proper input format will be subject to these additional charges.&lt;br /&gt;
 |-&lt;br /&gt;
 |colspan=2 style=&amp;quot;color: blue;&amp;quot; | High Throughput Setup&lt;br /&gt;
 |align=&amp;quot;center&amp;quot; style=&amp;quot;color: blue;&amp;quot; |48 samples &lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $60&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $66&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $84&lt;br /&gt;
 | &lt;br /&gt;
 |-&lt;br /&gt;
 |colspan=2|Sample Cleanup&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;|per sample&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $5&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $5.50&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $7&lt;br /&gt;
 |SPRI cleanup of samples to remove impurities. &lt;br /&gt;
 |-&lt;br /&gt;
 |colspan=2 style=&amp;quot;color: blue;&amp;quot; |Quantification&lt;br /&gt;
 |align=&amp;quot;center&amp;quot; style=&amp;quot;color: blue;&amp;quot; |per sample&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $10&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $11&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; style=&amp;quot;color: blue;&amp;quot; | $14&lt;br /&gt;
 |Quantification of samples (FA) prior to arraying.&lt;br /&gt;
 |-&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
.&lt;br /&gt;
&lt;br /&gt;
=== LONG READ LIBRARIES ===&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
 !Oxford Nanopore Sample Preparation&lt;br /&gt;
 !width=120| Nanopore Kit&lt;br /&gt;
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]&lt;br /&gt;
 !width=80| MIT&lt;br /&gt;
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]&lt;br /&gt;
 ! Notes&lt;br /&gt;
 |-&lt;br /&gt;
 |Standard Ligation || LSK || $160 || $176 || $240 &lt;br /&gt;
 |rowspan=6|Oxford Nanopore prep methods remain highly experimental and are continually in flux. &amp;lt;BR&amp;gt; As such, we can only estimate cost based on reagents/effort and cannot guarantee results.&amp;lt;BR&amp;gt; Please consult the core prior to sample submission for the latest information.&lt;br /&gt;
 |-&lt;br /&gt;
 |Native Barcoding &amp;lt;br&amp;gt; (ligation indexing) || - || $50 || $55 || $70&lt;br /&gt;
 |-&lt;br /&gt;
 |SMARTseq -&amp;gt; Ligation || Cv4+LSK || $300 || $330 || $420&lt;br /&gt;
 |-&lt;br /&gt;
 |Rapid Prep (tagment - ~10-20kb) || RAD || $150 || $165 || $225&lt;br /&gt;
 |-&lt;br /&gt;
 |Long Insert Prep || RAD (modified, target &amp;gt;100kb) || $262 || $289 || $393 &lt;br /&gt;
 |-&lt;br /&gt;
 |Direct RNA || RNA004 || $160 || $176 || $240 &lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
== SAMPLE EXTRACTION ==&lt;br /&gt;
=== CHEMAGIC360 ===&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
 !TYPE&lt;br /&gt;
 !width=120| Batch Size&lt;br /&gt;
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]&lt;br /&gt;
 !width=80| MIT&lt;br /&gt;
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]&lt;br /&gt;
 ! Notes&lt;br /&gt;
 |-&lt;br /&gt;
 |DNA&lt;br /&gt;
 |96&lt;br /&gt;
 |$700&lt;br /&gt;
 |$770&lt;br /&gt;
 |$980&lt;br /&gt;
 |&lt;br /&gt;
 |-&lt;br /&gt;
 |RNA&lt;br /&gt;
 |96&lt;br /&gt;
 |$800&lt;br /&gt;
 |$880&lt;br /&gt;
 |$1120&lt;br /&gt;
 |&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
== SINGLE CELL &amp;amp; SPATIAL SERVICES ==&lt;br /&gt;
=== 10X CHROMIUM ===&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
 ! 10X USAGE&lt;br /&gt;
 !width=120| Batch Size&lt;br /&gt;
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]&lt;br /&gt;
 !width=80| MIT&lt;br /&gt;
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]&lt;br /&gt;
 ! Notes&lt;br /&gt;
  |-&lt;br /&gt;
 |colspan=6 align=&amp;quot;center&amp;quot;|&#039;&#039;&#039;Assisted Service&#039;&#039;&#039;&lt;br /&gt;
 |- &lt;br /&gt;
 |colspan=6 |&#039;&#039;DIGITAL GENE EXPRESSION&#039;&#039;&lt;br /&gt;
 |- &lt;br /&gt;
 |rowspan=2 |5&#039; RNA &amp;lt;br&amp;gt; 3&#039; RNA&lt;br /&gt;
 |setup || $1,015 || $1,116.50 || $1,421 || charge per submission&lt;br /&gt;
 |- &lt;br /&gt;
 |per sample|| $1,750 || $1,925 || $2,450  || Includes QC of cDNA and creation of Short Read Library&lt;br /&gt;
 |- &lt;br /&gt;
 |rowspan=2 |5&#039; RNA &amp;lt;br&amp;gt; 3&#039; RNA - on chip multiplexing&lt;br /&gt;
 |setup || $1,150 || $1,265 || $1,1610 || charge per submission&lt;br /&gt;
 |- &lt;br /&gt;
 |per 4 samples|| $2,305 || $2,535 || $3,227  || Includes QC of cDNA and creation of Short Read Library&lt;br /&gt;
 |- &lt;br /&gt;
 |rowspan=2 |Fixed RNA (Flex v2)&amp;lt;br&amp;gt; probe based. human/mouse only&lt;br /&gt;
 |setup || $2700 || $2970 || $3780 || charge per submission - upto 48 samples&lt;br /&gt;
 |- &lt;br /&gt;
 |per sample || $270|| $297 || $378 || Includes QC of cDNA and creation of Short Read Library&lt;br /&gt;
 |-&lt;br /&gt;
 |rowspan=2 |Amplicon (CITE-SEQ, TCR, BCR, antibody barcocde, etc // per amplicon)&lt;br /&gt;
 |setup || $470 || $517 || $658 || additional prep beyond DGE&lt;br /&gt;
 |- &lt;br /&gt;
 |per sample|| $45 || $50 || $63  || Includes QC of cDNA and creation of Short Read Library&lt;br /&gt;
 |-&lt;br /&gt;
 |colspan=6 |&#039;&#039;scATACseq&#039;&#039;&lt;br /&gt;
 |- &lt;br /&gt;
 |rowspan=2 |ATACseq&lt;br /&gt;
 |setup || $880 || $968 || $1,232 || charge per submission&lt;br /&gt;
 |- &lt;br /&gt;
 |per sample|| $1,675|| $1,843|| $2,345|| Includes QC and creation of Short Read Library&lt;br /&gt;
 |-&lt;br /&gt;
 |colspan=6 |&#039;&#039;Multiome (ATAC+3&#039;RNA)&#039;&#039;&lt;br /&gt;
 |- &lt;br /&gt;
 |rowspan=2 |MULTI&lt;br /&gt;
 |setup || $1,800|| $1,980|| $2,520|| charge per submission&lt;br /&gt;
 |- &lt;br /&gt;
 |per sample|| $3,100|| $3,410|| $4,340|| Includes QC of cDNA and creation of Short Read Library&lt;br /&gt;
 |-&lt;br /&gt;
 |colspan=6 |&#039;&#039;CANCELLATION&#039;&#039;&lt;br /&gt;
 |-&lt;br /&gt;
 |per session || || $200|| $220|| $280|| &amp;lt;24h notice&lt;br /&gt;
|}&lt;br /&gt;
*please let BMC know if you plan to run this method early so we will have reagents ready for you.&lt;br /&gt;
&lt;br /&gt;
=== VISIUM ===&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
 ! SLIDES&lt;br /&gt;
 !width=120| Batch Size&lt;br /&gt;
 !width=80| KI Only&lt;br /&gt;
 !width=80| [[BioMicroCenter:CoreDeps|Other CORE LAB]]&lt;br /&gt;
 !width=80| MIT&lt;br /&gt;
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]&lt;br /&gt;
 ! Notes&lt;br /&gt;
 |- &lt;br /&gt;
 |Test Array ||slide|| $1,300|| $1,511|| $1,607|| $2435|| recommended 1x per tissue type for fresh frozen&lt;br /&gt;
 |- &lt;br /&gt;
 |Sample Slide - Fresh Frozen ||1 slide / 4 tiles|| $6,210|| $6,340|| $6,940|| $9,067 || Includes H&amp;amp;E stain images + Library Generation&lt;br /&gt;
 |- &lt;br /&gt;
 |Sample Slide - FFPE (hs/mm only) ||1 slide / 2 tiles|| $4,550|| $4,550|| $4,996|| $6,359 || Includes library generation only.&lt;br /&gt;
 |- &lt;br /&gt;
 |Sample Slide - HD (hs/mm only) ||1 slide / 2 tiles|| $7,850|| $7,850|| $8,596|| $10,940 || Includes library generation only.&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
=== AVITI24 ===&lt;br /&gt;
&amp;lt;div style=&amp;quot;background:#fff8e6; border-left:4px solid #c8960c; padding:8px 12px; margin-bottom:8px;&amp;quot;&amp;gt;&#039;&#039;&#039;Beta Service — Early Access:&#039;&#039;&#039; Consultation with BMC staff is required before submitting samples. See [[BioMicroCenter:SpTx|Spatial Transcriptomics]] for full service details.&amp;lt;/div&amp;gt;&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
 ! AVITI24 CYTOPROFILING / DISS&lt;br /&gt;
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]&lt;br /&gt;
 !width=80| MIT&lt;br /&gt;
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]&lt;br /&gt;
 !width=80| unit&lt;br /&gt;
 ! Notes&lt;br /&gt;
 |-&lt;br /&gt;
 |Atlas Panel (Teton CytoProfiling)&lt;br /&gt;
 |rowspan=5 align=center| per slide &amp;lt;BR&amp;gt; 1-, 12-, 48-well&lt;br /&gt;
 |align=right| $7,200&lt;br /&gt;
 |align=right| $7,920&lt;br /&gt;
 |align=right| $10,080&lt;br /&gt;
 |RNA + protein + morphology&lt;br /&gt;
 |-&lt;br /&gt;
 |Atlas Low (DISS — targeted in situ sequencing)&lt;br /&gt;
 |align=right| $5,500&lt;br /&gt;
 |align=right| $6,050&lt;br /&gt;
 |align=right| $7,700&lt;br /&gt;
 |User-designed probe; can run simultaneously with Atlas Panel&lt;br /&gt;
 |-&lt;br /&gt;
 |Antibody Add-on&lt;br /&gt;
 |align=right| $360&lt;br /&gt;
 |align=right| $396&lt;br /&gt;
 |align=right| $504&lt;br /&gt;
 |Custom antibody targets; consult BMC staff&lt;br /&gt;
 |-&lt;br /&gt;
 |Optimization Run&lt;br /&gt;
 |align=right| $800&lt;br /&gt;
 |align=right| $880&lt;br /&gt;
 |align=right| $1,120&lt;br /&gt;
 |Charged only if optimization slide fails&lt;br /&gt;
 |-&lt;br /&gt;
 |Slide Only (consumable)&lt;br /&gt;
 |align=right| $150&lt;br /&gt;
 |align=right| $165&lt;br /&gt;
 |align=right| $210&lt;br /&gt;
 |&lt;br /&gt;
|}&lt;br /&gt;
* High-output DISS (whole transcriptome) not yet released — contact BMC staff.&lt;br /&gt;
&lt;br /&gt;
== OTHER GENOMICS SERVICES ==&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br&amp;gt;&lt;br /&gt;
&lt;br /&gt;
=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
 ! QUALITY CONTROL&lt;br /&gt;
 !width=100| unit&lt;br /&gt;
 !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]&lt;br /&gt;
 !width=100| MIT &lt;br /&gt;
 !width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]&lt;br /&gt;
 ! Notes&lt;br /&gt;
 |-&lt;br /&gt;
 |Fragment Analyzer: RNA or DNA&lt;br /&gt;
 |per sample ||$15 || $16.50 || $21&lt;br /&gt;
 |&lt;br /&gt;
 |-&lt;br /&gt;
 |Fragment Analyzer: Preloaded row&lt;br /&gt;
 | per row (12) ||$80||$88 || $112&lt;br /&gt;
 | 2ul per sample in each well using plates from BMC&lt;br /&gt;
 |-&lt;br /&gt;
 |FemtoPulse: RNA or DNA&lt;br /&gt;
 |per sample || $30 || $33 || $42&lt;br /&gt;
 | &lt;br /&gt;
 |-&lt;br /&gt;
 |FemtoPulse: Preloaded Row&lt;br /&gt;
 |per row (12) || $160 || $176 || $224&lt;br /&gt;
 | 2ul per sample in each well using plates from BMC&lt;br /&gt;
 |-&lt;br /&gt;
 |BioAnalyzer: Walkup Service &lt;br /&gt;
 |per chip|| $50 || $55 || $70&lt;br /&gt;
 |  &lt;br /&gt;
 |-&lt;br /&gt;
 |qPCR only (Short read quantification test)&lt;br /&gt;
 |per sample || $25 || $27.50 || $35&lt;br /&gt;
 | 4 point serial dilution&lt;br /&gt;
 |-&lt;br /&gt;
 |Full plate qPCR (short read quantification test)&lt;br /&gt;
 |per plate || $400 || $440 || $560&lt;br /&gt;
 | full plate only (exclude column 1 for standards) - must be to BMC specifications.&lt;br /&gt;
 |-&lt;br /&gt;
 |colspan=6 align=&amp;quot;center&amp;quot;| &amp;lt;B&amp;gt; Sample QC services:&amp;lt;/B&amp;gt;&lt;br /&gt;
 |-&lt;br /&gt;
 |Fresh Frozen Curl&lt;br /&gt;
 |rowspan=2|per curl &lt;br /&gt;
 | $40|| $44 || $56&lt;br /&gt;
 |&lt;br /&gt;
 |-&lt;br /&gt;
 |FFPE Curl&lt;br /&gt;
 | $60 || $66 || $84&lt;br /&gt;
 |&lt;br /&gt;
 |- &lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
=== OLIGO SYNTHESIS ===&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
 !Platform&lt;br /&gt;
 !width=100| Service&lt;br /&gt;
 !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]&lt;br /&gt;
 !width=100| MIT &lt;br /&gt;
 !width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]&lt;br /&gt;
 ! Notes&lt;br /&gt;
 |-&lt;br /&gt;
 |rowspan=3| Dr.Oligo&lt;br /&gt;
 |Assisted&lt;br /&gt;
 |$2000&amp;lt;br&amp;gt;+consumables&lt;br /&gt;
 |$2200&amp;lt;br&amp;gt;+consumables&lt;br /&gt;
 |$2800&amp;lt;br&amp;gt;+consumables&lt;br /&gt;
 |includes setup and run of the instrument only. Large reagents / N2 included.&lt;br /&gt;
 |-&lt;br /&gt;
 |Walkup&lt;br /&gt;
 |$1000&amp;lt;br&amp;gt;+consumables&lt;br /&gt;
 |$1100&amp;lt;br&amp;gt;+consumables&lt;br /&gt;
 |$1400&amp;lt;br&amp;gt;+consumables&lt;br /&gt;
 |includes run of the instrument only. Large reagents / N2 included.&lt;br /&gt;
 |-&lt;br /&gt;
 |Training&lt;br /&gt;
 |$4000&lt;br /&gt;
 |$4400&lt;br /&gt;
 |$5600&lt;br /&gt;
 |ncludes two instrument runs. &lt;br /&gt;
 |-&lt;br /&gt;
 |rowspan=6| DNAscript Syntax&lt;br /&gt;
 |Setup (per run)&lt;br /&gt;
 |$1000&lt;br /&gt;
 |$1100&lt;br /&gt;
 |$1400&lt;br /&gt;
 |Per run cost&lt;br /&gt;
 |-&lt;br /&gt;
 |2000 nucleotide synthesis&lt;br /&gt;
 |$500&lt;br /&gt;
 |$550&lt;br /&gt;
 |$700&lt;br /&gt;
 |Up to 96 samples&lt;br /&gt;
 |-&lt;br /&gt;
 |8000 nucleotide synthesis&lt;br /&gt;
 |$1500&lt;br /&gt;
 |$1650&lt;br /&gt;
 |$2100&lt;br /&gt;
 |Up to 96 samples&lt;br /&gt;
 |-&lt;br /&gt;
 |add Biotin (max48)&lt;br /&gt;
 |$115&lt;br /&gt;
 |$126&lt;br /&gt;
 |$161&lt;br /&gt;
 |rowspan = 3| max 48 per run and 2 per oligo. Couple to T only.&lt;br /&gt;
 |-&lt;br /&gt;
 |add Fluor&lt;br /&gt;
 |$300&lt;br /&gt;
 |$330&lt;br /&gt;
 |$420&lt;br /&gt;
 |-&lt;br /&gt;
 |add quench&lt;br /&gt;
 |$100&lt;br /&gt;
 |$110&lt;br /&gt;
 |$140&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
== [[BioMicroCenter:RTPCR|REAL TIME PCR]] ==&lt;br /&gt;
&lt;br /&gt;
{| border=1&lt;br /&gt;
 ! OTHER LAB SERVICES&lt;br /&gt;
 !width=100| [[BioMicroCenter:CoreDeps|CORE LAB / MIT]]&lt;br /&gt;
 !width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]&lt;br /&gt;
 !width=100| unit&lt;br /&gt;
 ! SIGN UP&lt;br /&gt;
 ! Notes&lt;br /&gt;
 |-&lt;br /&gt;
 | Roche LightCycler 480&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $15&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| N/A&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;| per plate&lt;br /&gt;
 | [[BioMicroCenter:Forms#Calenders|CALENDAR]] *available for CORE only&lt;br /&gt;
 | Contact [[BioMicroCenter:People|Christopher Hallee]] for training&lt;br /&gt;
 |-&lt;br /&gt;
 | SYBR Green (KAPA/Roche)&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $45&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| N/A&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;| per ml&lt;br /&gt;
 |&lt;br /&gt;
 | Available for MIT only &lt;br /&gt;
 |-&lt;br /&gt;
 | 96-well PLATES&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $10&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| N/A&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;| per plate&lt;br /&gt;
 |&lt;br /&gt;
 | Available for MIT only&lt;br /&gt;
 |-&lt;br /&gt;
|}&lt;br /&gt;
.&lt;br /&gt;
&amp;lt;br&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== OTHER LAB SERVICES ==&lt;br /&gt;
{| border=1&lt;br /&gt;
 ! OTHER LAB SERVICES&lt;br /&gt;
 !width=100| [[BioMicroCenter:CoreDeps|CORE LAB / MIT]]&lt;br /&gt;
 !width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]&lt;br /&gt;
 !width=100| unit&lt;br /&gt;
 ! SIGN UP&lt;br /&gt;
 ! Notes&lt;br /&gt;
 |- &lt;br /&gt;
 |colspan=6 align=&amp;quot;center&amp;quot;| &#039;&#039;&#039;COVARIS SONICATOR&#039;&#039;&#039;&lt;br /&gt;
 |-&lt;br /&gt;
 |COVARIS E220&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;|$20&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;|$30&lt;br /&gt;
 |per hour&lt;br /&gt;
 |[https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar]&lt;br /&gt;
 |tubes available for purchase.&lt;br /&gt;
 |-&lt;br /&gt;
&lt;br /&gt;
 | colspan=&amp;quot;6&amp;quot; align=&amp;quot;center&amp;quot;| &#039;&#039;&#039;PIPPIN PREP&#039;&#039;&#039;&lt;br /&gt;
 |-&lt;br /&gt;
 |Gel Isolation&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $75&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $105&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;| per gel (5 lanes)&lt;br /&gt;
 | TBA&lt;br /&gt;
 | &lt;br /&gt;
 |-&lt;br /&gt;
 |Training&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $25&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $50&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;| per session&lt;br /&gt;
 | TBA&lt;br /&gt;
 | Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.&lt;br /&gt;
 |-&lt;br /&gt;
| colspan=&amp;quot;6&amp;quot; align=&amp;quot;center&amp;quot;| &#039;&#039;&#039;PLATE READER&#039;&#039;&#039;&lt;br /&gt;
 |-&lt;br /&gt;
 | Varioskan&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $18&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $27&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;| per hour&lt;br /&gt;
 | [[BioMicroCenter:Forms#Calenders|CALENDAR]]&lt;br /&gt;
 | Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training &lt;br /&gt;
 |-&lt;br /&gt;
 | colspan=&amp;quot;6&amp;quot; align=&amp;quot;center&amp;quot;| &#039;&#039;&#039;ROBOTICS&#039;&#039;&#039;&lt;br /&gt;
 |-&lt;br /&gt;
 | Tecan EVO 150&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $75&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $105&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;| per 1h block - based on scheduled time.&lt;br /&gt;
 | [[BioMicroCenter:Forms#Calenders|CALENDAR]]&lt;br /&gt;
 | &lt;br /&gt;
 |-&lt;br /&gt;
 |non-filter tips&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $8&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $11.20&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;| per box&lt;br /&gt;
 |&lt;br /&gt;
 |** Please let us know if there is additional plasticware you would like us to stock.&lt;br /&gt;
 |-&lt;br /&gt;
 |Filter tips&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $12.00&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $16.80&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;| per box&lt;br /&gt;
 |&lt;br /&gt;
 |&lt;br /&gt;
 |-&lt;br /&gt;
 | Training&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $65&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $91&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;| per hour&lt;br /&gt;
 | Contact [[BioMicroCenter:People|Tatum Murdock]]&lt;br /&gt;
 | &lt;br /&gt;
 |-&lt;br /&gt;
 | colspan=&amp;quot;6&amp;quot; align=&amp;quot;center&amp;quot;| &#039;&#039;&#039;NANODROP&#039;&#039;&#039;&lt;br /&gt;
 |-&lt;br /&gt;
 | Nanodrop&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| --&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| --&lt;br /&gt;
 |align=&amp;quot;center&amp;quot;| &lt;br /&gt;
 | Walk up service&lt;br /&gt;
 | * no charge&lt;br /&gt;
 |-&lt;br /&gt;
|}&lt;br /&gt;
&amp;lt;br&amp;gt;&amp;lt;br&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== COMPUTATIONAL SERVICES ==   &lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
 ! COMPUTATIONAL SERVICES&lt;br /&gt;
 !width=200| unit&lt;br /&gt;
 !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]&lt;br /&gt;
 !width=100| Other MIT / non-MIT &lt;br /&gt;
 ! Notes&lt;br /&gt;
  |-&lt;br /&gt;
 | Informatics Support&lt;br /&gt;
 | align=&amp;quot;right&amp;quot;| per hour&lt;br /&gt;
 | align=&amp;quot;right&amp;quot;| $90&lt;br /&gt;
 | align=&amp;quot;right&amp;quot;| $125 / $205&lt;br /&gt;
 | Very limited support for non-MIT users&lt;br /&gt;
 |-&lt;br /&gt;
 | Informatics Share&lt;br /&gt;
 | align=&amp;quot;right&amp;quot;| per 0.1FTE&lt;br /&gt;
 | align=&amp;quot;right&amp;quot;| $1,250&lt;br /&gt;
 | align=&amp;quot;right&amp;quot;| N/A&lt;br /&gt;
 | MIT only. Priority access to informatics.&lt;br /&gt;
 |-&lt;br /&gt;
 |Ingenuity Pathway Analysis&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;|per dataset&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; | $100&lt;br /&gt;
 |align=&amp;quot;right&amp;quot; | $110/$150&lt;br /&gt;
 | Recovery of licence cost.&lt;br /&gt;
 |-&lt;br /&gt;
 |Active Data storage&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| per TB per year&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $100&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $300 / N/A&lt;br /&gt;
 |  &lt;br /&gt;
 |-&lt;br /&gt;
 | Tape Archive / Recovery&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| per tape&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $60&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $70&lt;br /&gt;
 | &lt;br /&gt;
 |-&lt;br /&gt;
 | Informatics Training Classes&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| per session&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $50&lt;br /&gt;
 |align=&amp;quot;right&amp;quot;| $100&lt;br /&gt;
 | &lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
OLD&amp;lt;BR&amp;gt;&lt;br /&gt;
[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]]&amp;lt;BR&amp;gt;&lt;br /&gt;
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]]&amp;lt;BR&amp;gt;&lt;br /&gt;
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]]&amp;lt;BR&amp;gt;&lt;br /&gt;
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]]&amp;lt;BR&amp;gt;&lt;br /&gt;
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]]&amp;lt;BR&amp;gt;&lt;br /&gt;
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]]&amp;lt;BR&amp;gt;&lt;br /&gt;
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]]&amp;lt;BR&amp;gt;&lt;br /&gt;
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]]&amp;lt;BR&amp;gt;&lt;br /&gt;
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]]&amp;lt;BR&amp;gt;&lt;br /&gt;
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]]&amp;lt;BR&amp;gt;&lt;br /&gt;
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]]&amp;lt;BR&amp;gt;&lt;br /&gt;
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]]&amp;lt;BR&amp;gt;&lt;br /&gt;
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:SpTx&amp;diff=131220</id>
		<title>BioMicroCenter:SpTx</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:SpTx&amp;diff=131220"/>
		<updated>2026-03-22T20:22:06Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: Stack Visium HD workflow image above intro text using full-width table row&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;{{BioMicroCenter}}&lt;br /&gt;
 &lt;br /&gt;
The BioMicro Center supports two complementary approaches to spatial transcriptomics: [[BioMicroCenter:SpTx#10X Visium HD|10x Visium HD]] from 10x Genomics for slide-based spatial gene expression, and [[BioMicroCenter:SpTx#AVITI24 In Situ Sequencing|AVITI24 in situ sequencing]] from Element Biosciences. The choice of method depends on your tissue type, resolution requirements, and experimental goals. We strongly encourage consultation with BMC staff prior to beginning a spatial experiment.&lt;br /&gt;
 &lt;br /&gt;
== 10X VISIUM HD ==&lt;br /&gt;
{| width=&amp;quot;100%&amp;quot;&lt;br /&gt;
|-&lt;br /&gt;
| colspan=&amp;quot;2&amp;quot; |&lt;br /&gt;
[[File:VisiumHD_workflow_slide.jpg|left|250px|Workflow and capture areas for Visium HD]]&lt;br /&gt;
&amp;lt;div style=&amp;quot;clear:both; padding-top:4px; font-size:12px; color:#555;&amp;quot;&amp;gt;Workflow and capture areas for Visium HD&amp;lt;/div&amp;gt;&lt;br /&gt;
|-&lt;br /&gt;
| colspan=&amp;quot;2&amp;quot; |&lt;br /&gt;
[https://www.10xgenomics.com/products/visium-hd-spatial-gene-expression 10x Visium HD] is a spatial transcriptomics platform that profiles whole-transcriptome gene expression across intact tissue sections at single-cell-scale resolution. Tissue sections are processed through the CytAssist instrument, which transfers RNA complexes onto a capture slide containing a continuous array of 3,000,000 spatially barcoded 2 µm squares covering a 6.5 × 6.5 mm capture area (~0.65 cm²). Two chemistries are available: a probe-based assay for human and mouse (FF, FxF, and FFPE), and a polyA-based assay (Visium HD 3&#039;) for fresh frozen tissue from any species. H&amp;amp;E or immunofluorescence (IF) staining can be performed prior to processing for protein co-detection.&lt;br /&gt;
&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
|}&lt;br /&gt;
 &lt;br /&gt;
{|&lt;br /&gt;
 |- style=&amp;quot;vertical-align: top;&amp;quot;&lt;br /&gt;
 |style=&amp;quot;width: 500px;&amp;quot;|&lt;br /&gt;
 &lt;br /&gt;
=== ARRAY SPECIFICATIONS ===&lt;br /&gt;
 {| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
  ! Capture area&lt;br /&gt;
  | 6.5 × 6.5 mm (~0.65 cm²) per tissue&lt;br /&gt;
  |-&lt;br /&gt;
  ! Spots&lt;br /&gt;
  | 3,000,000 barcoded squares&lt;br /&gt;
  |-&lt;br /&gt;
  ! Spot size&lt;br /&gt;
  | 2 µm continuous (no gaps)&lt;br /&gt;
  |-&lt;br /&gt;
  ! Default analysis bins&lt;br /&gt;
  | 8 µm and 16 µm (SpaceRanger); custom bins supported&lt;br /&gt;
  |-&lt;br /&gt;
  ! Tissues per slide&lt;br /&gt;
  | 2&lt;br /&gt;
  |-&lt;br /&gt;
  ! Multiomics&lt;br /&gt;
  | H&amp;amp;E or IF staining prior to processing; antibody panels (FFPE, human only)&lt;br /&gt;
 |}&lt;br /&gt;
 &lt;br /&gt;
=== CHEMISTRY OPTIONS ===&lt;br /&gt;
 {| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
  !&lt;br /&gt;
  ! Visium HD (probe-based)&lt;br /&gt;
  ! Visium HD 3&#039; (polyA-based)&lt;br /&gt;
  |-&lt;br /&gt;
  ! Species&lt;br /&gt;
  | Human, Mouse only&lt;br /&gt;
  | Any species&lt;br /&gt;
  |-&lt;br /&gt;
  ! Tissue formats&lt;br /&gt;
  | Fresh Frozen (FF), Fixed Frozen (FxF), FFPE&lt;br /&gt;
  | Fresh Frozen (FF) only&lt;br /&gt;
  |-&lt;br /&gt;
  ! Notes&lt;br /&gt;
  | ~3 probes per gene; exogenous sequences (GFP, reporters) require custom probes. A small fraction of genes are excluded by default due to predicted off-target activity: [https://www.10xgenomics.com/support/spatial-gene-expression-hd/documentation/steps/probe-sets/visium-human-transcriptome-probe-set-v2-1 human excluded gene list] (~2.1%), [https://www.10xgenomics.com/support/spatial-gene-expression-hd/documentation/steps/probe-sets/visium-mouse-transcriptome-probe-set-v2-0 mouse excluded gene list] (~1.8%).&lt;br /&gt;
  | Species-agnostic; captures polyadenylated transcripts&lt;br /&gt;
 |}&lt;br /&gt;
 &lt;br /&gt;
=== TISSUE PREPARATION REQUIREMENTS ===&lt;br /&gt;
 {| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
  !&lt;br /&gt;
  ! Fresh Frozen (FF)&lt;br /&gt;
  ! Fixed Frozen (FxF)&lt;br /&gt;
  ! FFPE&lt;br /&gt;
  |-&lt;br /&gt;
  ! Section thickness&lt;br /&gt;
  | 10–20 µm &amp;lt;BR&amp;gt;&amp;lt;small&amp;gt;10 µm recommended&amp;lt;/small&amp;gt;&lt;br /&gt;
  | 10–20 µm&lt;br /&gt;
  | 5 µm&lt;br /&gt;
  |-&lt;br /&gt;
  ! Storage&lt;br /&gt;
  | OCT, −80°C&lt;br /&gt;
  | −80°C&lt;br /&gt;
  | Room temp&lt;br /&gt;
  |-&lt;br /&gt;
  ! RNA quality&lt;br /&gt;
  | colspan=&amp;quot;3&amp;quot; | DV200 ≥ 30% recommended; higher scores improve sensitivity&lt;br /&gt;
  |-&lt;br /&gt;
  ! Histology&lt;br /&gt;
  | colspan=&amp;quot;3&amp;quot; | Sectioning &amp;amp; H&amp;amp;E performed by the [https://ki-sbc.mit.edu/histology Tang Histology Facility] (KI). Coordinate with BMC staff to arrange.&lt;br /&gt;
 |}&lt;br /&gt;
 &lt;br /&gt;
For current pricing, see [[BioMicroCenter:Pricing]] → Single Cell &amp;amp; Spatial Services → Visium. Sequencing is charged separately; 400–800M reads per tile recommended for Visium HD.&lt;br /&gt;
 &lt;br /&gt;
=== SUBMISSION ===&lt;br /&gt;
Submit via [https://mit.ilabsolutions.com/service_item/new/3381?spt_id=12903 iLabs (assisted service)]. Coordinate with BMC staff at least one week in advance. Tissue processing begins at the [https://ki-sbc.mit.edu/histology Tang Histology Facility]; please arrange sectioning separately.&lt;br /&gt;
 &lt;br /&gt;
|&lt;br /&gt;
 &lt;br /&gt;
=== 10X Visium HD ===&lt;br /&gt;
[[File:VisiumHD_loupe_example.jpg|thumb|right|500px|Example of FF data from Visium HD]]&lt;br /&gt;
 &lt;br /&gt;
&#039;&#039;&#039;FAQs for Users&#039;&#039;&#039;&amp;lt;BR&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;1) What tissues are compatible?&#039;&#039;&#039;&amp;lt;br&amp;gt;&lt;br /&gt;
Visium HD (probe-based) supports human and mouse tissues in FF, FxF, and FFPE formats. Visium HD 3&#039; (polyA) supports fresh frozen tissue from any species. 10x Genomics maintains a [https://www.10xgenomics.com/support/spatial-gene-expression-hd/documentation/steps/tissue-prep-for-fresh-frozen/visium-hd-spatial-gene-expression-tested-tissues-fresh-frozen list of tested tissues]. Additional optimization may be required for tissues with high fat content. Consult BMC staff before starting.&lt;br /&gt;
&amp;lt;br&amp;gt;&amp;lt;br&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;2) How do I prepare and submit tissue?&#039;&#039;&#039;&amp;lt;br&amp;gt;&lt;br /&gt;
Tissue sectioning and H&amp;amp;E staining are performed by the [https://ki-sbc.mit.edu/histology Hope Babette Tang (1983) Histology Facility] at the Koch Institute. Coordinate with both the Histology Facility and BMC staff before your first submission. FF tissue should be embedded in OCT and stored at −80°C; FFPE blocks at room temperature.&lt;br /&gt;
&amp;lt;br&amp;gt;&amp;lt;br&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;3) What is the resolution?&#039;&#039;&#039;&amp;lt;br&amp;gt;&lt;br /&gt;
Visium HD captures data at 2 µm bin resolution (single-cell scale). SpaceRanger aggregates into 8 µm and 16 µm bins for higher sensitivity by default; custom bin sizes are also supported. Cell segmentation based on H&amp;amp;E imaging is available in the Loupe Browser pipeline.&lt;br /&gt;
&amp;lt;br&amp;gt;&amp;lt;br&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;4) How much sequencing is needed?&#039;&#039;&#039;&amp;lt;br&amp;gt;&lt;br /&gt;
400–800M reads per tile is recommended for Visium HD. Sequencing is submitted separately via iLabs. Compatible platforms include NovaSeq SP, NovaSeqX, and Element AVITI. Discuss read depth with BMC staff based on your tissue type and goals.&lt;br /&gt;
&amp;lt;br&amp;gt;&amp;lt;br&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;5) Does Visium HD require permeabilization optimization?&#039;&#039;&#039;&amp;lt;br&amp;gt;&lt;br /&gt;
No. Visium HD does not require permeabilization time optimization. For fresh frozen tissues new to the platform, a test array is available to verify tissue quality and placement before committing a full experiment.&lt;br /&gt;
&amp;lt;br&amp;gt;&amp;lt;br&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;6) Can I detect proteins alongside gene expression?&#039;&#039;&#039;&amp;lt;br&amp;gt;&lt;br /&gt;
Yes. H&amp;amp;E or IF staining can be performed prior to CytAssist processing. Antibody panels for protein co-detection (human tissues, FFPE only) are available from 10x Genomics. Discuss your experimental design with BMC staff in advance.&lt;br /&gt;
&amp;lt;br&amp;gt;&amp;lt;br&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;7) What analysis outputs are provided?&#039;&#039;&#039;&amp;lt;br&amp;gt;&lt;br /&gt;
The BMC delivers FASTQ files, aligned BAM files, the spatial feature-barcode matrix (raw and filtered at 2, 8, and 16 µm bins), and a Loupe Browser file. Downstream informatics support is available through BMC staff.&lt;br /&gt;
&amp;lt;br&amp;gt;&amp;lt;br&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;8) Can I combine Visium HD with single-cell data?&#039;&#039;&#039;&amp;lt;br&amp;gt;&lt;br /&gt;
Yes. Visium HD data integrates with single-cell or single-nucleus RNA-seq (e.g., 10x Chromium Flex) for cell-type deconvolution. Starting from the same tissue block, nuclei can be isolated for Flex single-cell gene expression. Coordinate with BMC staff if planning a multi-modal experiment.&lt;br /&gt;
&amp;lt;br&amp;gt;&amp;lt;br&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;9) My gene of interest is exogenous (GFP, reporter, viral) or missing from the probe set. What are my options?&#039;&#039;&#039;&amp;lt;br&amp;gt;&lt;br /&gt;
The probe-based Visium HD assay does not capture exogenous sequences by default. Custom probes can be designed for detection — see 10x Genomics technical note CG000621. Additionally, a small fraction of endogenous genes are excluded from the standard probe sets due to predicted off-target activity: see the [https://www.10xgenomics.com/support/spatial-gene-expression-hd/documentation/steps/probe-sets/visium-human-transcriptome-probe-set-v2-1 human excluded gene list] (~2.1% of genes) and [https://www.10xgenomics.com/support/spatial-gene-expression-hd/documentation/steps/probe-sets/visium-mouse-transcriptome-probe-set-v2-0 mouse excluded gene list] (~1.8% of genes). Alternatively, the polyA-based Visium HD 3&#039; assay captures all polyadenylated transcripts and does not require custom probes.&lt;br /&gt;
&amp;lt;br&amp;gt;&lt;br /&gt;
 &lt;br /&gt;
|}&lt;br /&gt;
 &lt;br /&gt;
== AVITI24 CYTOPROFILING &amp;amp; IN SITU SEQUENCING ==&lt;br /&gt;
&amp;lt;div style=&amp;quot;background:#fff8e6; border-left:4px solid #c8960c; padding:8px 12px; margin-bottom:12px;&amp;quot;&amp;gt;&#039;&#039;&#039;Beta Service — Early Access:&#039;&#039;&#039; The AVITI24 Teton CytoProfiling and DISS services at the BioMicro Center are operational but not yet routine. Parameters, pricing, and workflows are still being established. &#039;&#039;&#039;Consultation with BMC staff is required before submitting samples.&#039;&#039;&#039;&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;!-- placeholder image left: AVITI24 instrument photo or Teton CytoProfiling schematic --&amp;gt;&lt;br /&gt;
&amp;lt;!-- replace with: [[File:AVITI24_instrument.png|thumb|left|400px]] --&amp;gt;&lt;br /&gt;
&lt;br /&gt;
The BioMicro Center provides access to the [https://www.elementbiosciences.com/products/aviti24 Element Biosciences AVITI24] platform for in situ single-cell multiomics. Using [https://www.elementbiosciences.com/products/aviti24/cytoprofiling Teton™ CytoProfiling], the instrument co-detects RNA transcripts, proteins (including phospho-proteins), and cell morphology at subcellular resolution — directly in intact cells, without library preparation. Targeted in situ sequencing via Direct In Sample Sequencing (DISS) can be run simultaneously in the same experiment. Because this is an early-access service, results and run parameters are less well-established than our established offerings. Consultation with BMC staff is strongly encouraged before designing experiments.&lt;br /&gt;
&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
&lt;br /&gt;
{|&lt;br /&gt;
 |- style=&amp;quot;vertical-align: top;&amp;quot;&lt;br /&gt;
 |style=&amp;quot;width: 500px;&amp;quot;|&lt;br /&gt;
&lt;br /&gt;
=== PLATFORM SPECIFICATIONS ===&lt;br /&gt;
 {| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
  ! Instrument&lt;br /&gt;
  | Element Biosciences AVITI24&lt;br /&gt;
  |-&lt;br /&gt;
  ! Technology&lt;br /&gt;
  | Teton™ CytoProfiling (ABC Sequencing)&lt;br /&gt;
  |-&lt;br /&gt;
  ! Flow cell formats&lt;br /&gt;
  | 1-well · 12-well (96-well format) · 48-well (384-well format)&lt;br /&gt;
  |-&lt;br /&gt;
  ! Imageable area&lt;br /&gt;
  | Up to 10 cm² per flow cell side&lt;br /&gt;
  |-&lt;br /&gt;
  ! Max cells per run&lt;br /&gt;
  | Up to 1 million adherent cells per flow cell&lt;br /&gt;
  |-&lt;br /&gt;
  ! Transcript density&lt;br /&gt;
  | ~100–200 transcripts per 100 µm²&lt;br /&gt;
 |}&lt;br /&gt;
&lt;br /&gt;
=== ASSAY CAPABILITIES ===&lt;br /&gt;
 {| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
  ! style=&amp;quot;width:18%&amp;quot;|&lt;br /&gt;
  ! style=&amp;quot;width:27%; background:#e8f0f8;&amp;quot;| Teton CytoProfiling&lt;br /&gt;
  ! style=&amp;quot;width:27%; background:#e8f4e8;&amp;quot;| DISS (low output) &amp;lt;BR&amp;gt;&amp;lt;small&amp;gt;&#039;&#039;&#039;available&#039;&#039;&#039;&amp;lt;/small&amp;gt;&lt;br /&gt;
  ! style=&amp;quot;width:27%; background:#f5f0f0;&amp;quot;| DISS (high output) &amp;lt;BR&amp;gt;&amp;lt;small&amp;gt;&#039;&#039;not yet released&#039;&#039;&amp;lt;/small&amp;gt;&lt;br /&gt;
  |-&lt;br /&gt;
  ! RNA&lt;br /&gt;
  | 350 transcripts &amp;lt;BR&amp;gt;&amp;lt;small&amp;gt;panel-based&amp;lt;/small&amp;gt;&lt;br /&gt;
  | Targeted genes &amp;lt;BR&amp;gt;&amp;lt;small&amp;gt;~50 nt, 1 priming event; CRISPR guides, barcodes, expressed mutations&amp;lt;/small&amp;gt;&lt;br /&gt;
  | &amp;lt;span style=&amp;quot;color:#999;&amp;quot;&amp;gt;Whole transcriptome &amp;lt;BR&amp;gt;&amp;lt;small&amp;gt;polyT prime; 100–300 nt&amp;lt;/small&amp;gt;&amp;lt;/span&amp;gt;&lt;br /&gt;
  |-&lt;br /&gt;
  ! Protein&lt;br /&gt;
  | colspan=&amp;quot;3&amp;quot; | ~18 targets &amp;amp;nbsp;&amp;lt;small&amp;gt;panels of 24; rabbit monoclonal primary; custom available&amp;lt;/small&amp;gt;&lt;br /&gt;
  |-&lt;br /&gt;
  ! Morphology&lt;br /&gt;
  | colspan=&amp;quot;3&amp;quot; | Nucleus, actin, membrane (all species) · ER, Golgi, mitochondria (human only)&lt;br /&gt;
  |-&lt;br /&gt;
  ! Panels / probes&lt;br /&gt;
  | MAPK Cell Cycle · MAPK Apoptosis · Immuno-Oncology · Neuroscience &amp;lt;BR&amp;gt;&amp;lt;small&amp;gt;mix up to 3 panels per run; consult staff for latest&amp;lt;/small&amp;gt;&lt;br /&gt;
  | User-designed probes&lt;br /&gt;
  | &amp;lt;span style=&amp;quot;color:#999;&amp;quot;&amp;gt;User-designed or polyT probe&amp;lt;/span&amp;gt;&lt;br /&gt;
 |}&lt;br /&gt;
Teton CytoProfiling and DISS (low output) can be run simultaneously in the same experiment. Custom antibody add-ons require unconjugated rabbit monoclonal primaries. Consult BMC staff for current options.&lt;br /&gt;
&lt;br /&gt;
=== SAMPLE REQUIREMENTS ===&lt;br /&gt;
 {| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
  ! Supported&lt;br /&gt;
  | Monolayer cell culture (adherent); pelleted cell suspension (spun down and resuspended)&lt;br /&gt;
  |-&lt;br /&gt;
  ! Not yet supported&lt;br /&gt;
  | Complex 3D culture, organoids, tissue sections — contact BMC to discuss future availability&lt;br /&gt;
  |-&lt;br /&gt;
  ! Cell density&lt;br /&gt;
  | Consult BMC staff; optimized per cell type&lt;br /&gt;
  |-&lt;br /&gt;
  ! Species&lt;br /&gt;
  | Human (all current fixed panels); other species — consult staff regarding panel compatibility&lt;br /&gt;
 |}&lt;br /&gt;
&lt;br /&gt;
=== SUBMISSION ===&lt;br /&gt;
&amp;lt;!-- iLabs link stub: replace once service item is created --&amp;gt;&lt;br /&gt;
&amp;lt;!-- [https://mit.ilabsolutions.com/service_item/new/XXXX iLabs (assisted service)] --&amp;gt;&lt;br /&gt;
Submission via iLabs is not yet available. Contact BMC staff directly to discuss your experiment before scheduling. &#039;&#039;&#039;Consultation is required for all AVITI24 Teton and DISS submissions.&#039;&#039;&#039;&lt;br /&gt;
&lt;br /&gt;
|&lt;br /&gt;
&lt;br /&gt;
=== AVITI24 Teton CytoProfiling at BMC ===&lt;br /&gt;
[[File:AVITI24_Boyer_cardiomyocytes.jpg|thumb|right|300px|Cytoprofiling of cardiomyocytes. Collaboration with the Boyer Lab.]]&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;About the Service&#039;&#039;&#039;&amp;lt;BR&amp;gt;&lt;br /&gt;
The AVITI24 at BMC supports Teton™ CytoProfiling — an in situ multiomics approach that co-detects RNA transcripts, proteins (including phospho-proteins), and cell morphology directly inside intact cells on the flow cell. Each run cycles through Cell Painting (morphology imaging), antibody scoring, and in situ sequencing rounds without any library preparation. A full run takes 24–48 hours end-to-end with approximately 45 minutes of hands-on time, capturing ~100–200 transcripts per 100 µm² at subcellular resolution.&lt;br /&gt;
&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
The platform is designed for cells in monolayer culture or spun-down suspension. It profiles up to 350 RNA targets, ~18 protein targets, and 6 morphology channels simultaneously in a single experiment — giving a co-registered multiomic readout per cell that would otherwise require separate assays on separate instruments.&lt;br /&gt;
&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
Targeted in situ sequencing (DISS, low output) can be layered onto any Teton run. This uses a user-designed probe (~50 nt, 1 priming event) to detect specific sequences in situ — useful for CRISPR guide detection, barcode readout, or expressed mutation calling. Note that reads are too short for TCR/BCR sequencing; consult BMC staff if your application involves immune repertoire.&lt;br /&gt;
&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;Available Assays&#039;&#039;&#039;&amp;lt;BR&amp;gt;&lt;br /&gt;
BMC supports all four currently available fixed Teton panels (MAPK Cell Cycle, MAPK Apoptosis, Immuno-Oncology, Neuroscience). Up to three panels can be combined per run, giving up to 350 RNA targets and ~18 usable protein targets in a single experiment. Custom antibody add-ons are possible using unconjugated rabbit monoclonal primary antibodies — no conjugation or barcoding required. Discuss panel selection with BMC staff; reagent availability and lead time vary.&lt;br /&gt;
&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;Data Outputs&#039;&#039;&#039;&amp;lt;BR&amp;gt;&lt;br /&gt;
The BMC provides core analysis outputs including:&lt;br /&gt;
* Cell-by-gene expression matrix (350 transcript targets per panel)&lt;br /&gt;
* Cell-by-protein parameter data (~18 antibody targets)&lt;br /&gt;
* Morphology feature data per cell (nucleus, actin, membrane; ER, Golgi, mitochondria for human)&lt;br /&gt;
* Spatial maps with morphology channel overlays&lt;br /&gt;
* [https://www.elementbiosciences.com/products/elembio-catalyst ElemBio Catalyst / CytoCanvas]-compatible files for interactive visualization and downstream analysis&lt;br /&gt;
&amp;lt;BR&amp;gt;&lt;br /&gt;
Additional informatics support is available through BMC staff.&lt;br /&gt;
&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;Pricing&#039;&#039;&#039;&amp;lt;BR&amp;gt;&lt;br /&gt;
Pricing for AVITI24 Teton CytoProfiling and DISS is not yet finalized. See [[BioMicroCenter:Pricing]] for updates as pricing is established. Sequencing-only runs on the AVITI24 are charged at standard AVITI24 sequencing rates.&lt;br /&gt;
&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;Service Status&#039;&#039;&#039;&amp;lt;BR&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;background:#fff8e6; border-left:4px solid #c8960c; padding:8px 12px; margin-top:8px;&amp;quot;&amp;gt;This service is in &#039;&#039;&#039;beta&#039;&#039;&#039;. The instrument is operational and BMC has completed initial runs, but experimental parameters are still being validated. We welcome early users willing to work collaboratively with BMC staff to establish protocols.&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
|}&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
	<entry>
		<id>http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:SpTx&amp;diff=131219</id>
		<title>BioMicroCenter:SpTx</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:SpTx&amp;diff=131219"/>
		<updated>2026-03-22T20:19:01Z</updated>

		<summary type="html">&lt;p&gt;Asoberan: Resize images: workflow 250px, Boyer cardiomyocytes 300px&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;{{BioMicroCenter}}&lt;br /&gt;
 &lt;br /&gt;
The BioMicro Center supports two complementary approaches to spatial transcriptomics: [[BioMicroCenter:SpTx#10X Visium HD|10x Visium HD]] from 10x Genomics for slide-based spatial gene expression, and [[BioMicroCenter:SpTx#AVITI24 In Situ Sequencing|AVITI24 in situ sequencing]] from Element Biosciences. The choice of method depends on your tissue type, resolution requirements, and experimental goals. We strongly encourage consultation with BMC staff prior to beginning a spatial experiment.&lt;br /&gt;
 &lt;br /&gt;
== 10X VISIUM HD ==&lt;br /&gt;
[[File:VisiumHD_workflow_slide.jpg|thumb|left|250px|Workflow and capture areas for Visium HD]]&lt;br /&gt;
 &lt;br /&gt;
[https://www.10xgenomics.com/products/visium-hd-spatial-gene-expression 10x Visium HD] is a spatial transcriptomics platform that profiles whole-transcriptome gene expression across intact tissue sections at single-cell-scale resolution. Tissue sections are processed through the CytAssist instrument, which transfers RNA complexes onto a capture slide containing a continuous array of 3,000,000 spatially barcoded 2 µm squares covering a 6.5 × 6.5 mm capture area (~0.65 cm²). Two chemistries are available: a probe-based assay for human and mouse (FF, FxF, and FFPE), and a polyA-based assay (Visium HD 3&#039;) for fresh frozen tissue from any species. H&amp;amp;E or immunofluorescence (IF) staining can be performed prior to processing for protein co-detection.&lt;br /&gt;
&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
 &lt;br /&gt;
{|&lt;br /&gt;
 |- style=&amp;quot;vertical-align: top;&amp;quot;&lt;br /&gt;
 |style=&amp;quot;width: 500px;&amp;quot;|&lt;br /&gt;
 &lt;br /&gt;
=== ARRAY SPECIFICATIONS ===&lt;br /&gt;
 {| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
  ! Capture area&lt;br /&gt;
  | 6.5 × 6.5 mm (~0.65 cm²) per tissue&lt;br /&gt;
  |-&lt;br /&gt;
  ! Spots&lt;br /&gt;
  | 3,000,000 barcoded squares&lt;br /&gt;
  |-&lt;br /&gt;
  ! Spot size&lt;br /&gt;
  | 2 µm continuous (no gaps)&lt;br /&gt;
  |-&lt;br /&gt;
  ! Default analysis bins&lt;br /&gt;
  | 8 µm and 16 µm (SpaceRanger); custom bins supported&lt;br /&gt;
  |-&lt;br /&gt;
  ! Tissues per slide&lt;br /&gt;
  | 2&lt;br /&gt;
  |-&lt;br /&gt;
  ! Multiomics&lt;br /&gt;
  | H&amp;amp;E or IF staining prior to processing; antibody panels (FFPE, human only)&lt;br /&gt;
 |}&lt;br /&gt;
 &lt;br /&gt;
=== CHEMISTRY OPTIONS ===&lt;br /&gt;
 {| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
  !&lt;br /&gt;
  ! Visium HD (probe-based)&lt;br /&gt;
  ! Visium HD 3&#039; (polyA-based)&lt;br /&gt;
  |-&lt;br /&gt;
  ! Species&lt;br /&gt;
  | Human, Mouse only&lt;br /&gt;
  | Any species&lt;br /&gt;
  |-&lt;br /&gt;
  ! Tissue formats&lt;br /&gt;
  | Fresh Frozen (FF), Fixed Frozen (FxF), FFPE&lt;br /&gt;
  | Fresh Frozen (FF) only&lt;br /&gt;
  |-&lt;br /&gt;
  ! Notes&lt;br /&gt;
  | ~3 probes per gene; exogenous sequences (GFP, reporters) require custom probes. A small fraction of genes are excluded by default due to predicted off-target activity: [https://www.10xgenomics.com/support/spatial-gene-expression-hd/documentation/steps/probe-sets/visium-human-transcriptome-probe-set-v2-1 human excluded gene list] (~2.1%), [https://www.10xgenomics.com/support/spatial-gene-expression-hd/documentation/steps/probe-sets/visium-mouse-transcriptome-probe-set-v2-0 mouse excluded gene list] (~1.8%).&lt;br /&gt;
  | Species-agnostic; captures polyadenylated transcripts&lt;br /&gt;
 |}&lt;br /&gt;
 &lt;br /&gt;
=== TISSUE PREPARATION REQUIREMENTS ===&lt;br /&gt;
 {| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
  !&lt;br /&gt;
  ! Fresh Frozen (FF)&lt;br /&gt;
  ! Fixed Frozen (FxF)&lt;br /&gt;
  ! FFPE&lt;br /&gt;
  |-&lt;br /&gt;
  ! Section thickness&lt;br /&gt;
  | 10–20 µm &amp;lt;BR&amp;gt;&amp;lt;small&amp;gt;10 µm recommended&amp;lt;/small&amp;gt;&lt;br /&gt;
  | 10–20 µm&lt;br /&gt;
  | 5 µm&lt;br /&gt;
  |-&lt;br /&gt;
  ! Storage&lt;br /&gt;
  | OCT, −80°C&lt;br /&gt;
  | −80°C&lt;br /&gt;
  | Room temp&lt;br /&gt;
  |-&lt;br /&gt;
  ! RNA quality&lt;br /&gt;
  | colspan=&amp;quot;3&amp;quot; | DV200 ≥ 30% recommended; higher scores improve sensitivity&lt;br /&gt;
  |-&lt;br /&gt;
  ! Histology&lt;br /&gt;
  | colspan=&amp;quot;3&amp;quot; | Sectioning &amp;amp; H&amp;amp;E performed by the [https://ki-sbc.mit.edu/histology Tang Histology Facility] (KI). Coordinate with BMC staff to arrange.&lt;br /&gt;
 |}&lt;br /&gt;
 &lt;br /&gt;
For current pricing, see [[BioMicroCenter:Pricing]] → Single Cell &amp;amp; Spatial Services → Visium. Sequencing is charged separately; 400–800M reads per tile recommended for Visium HD.&lt;br /&gt;
 &lt;br /&gt;
=== SUBMISSION ===&lt;br /&gt;
Submit via [https://mit.ilabsolutions.com/service_item/new/3381?spt_id=12903 iLabs (assisted service)]. Coordinate with BMC staff at least one week in advance. Tissue processing begins at the [https://ki-sbc.mit.edu/histology Tang Histology Facility]; please arrange sectioning separately.&lt;br /&gt;
 &lt;br /&gt;
|&lt;br /&gt;
 &lt;br /&gt;
=== 10X Visium HD ===&lt;br /&gt;
[[File:VisiumHD_loupe_example.jpg|thumb|right|500px|Example of FF data from Visium HD]]&lt;br /&gt;
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&#039;&#039;&#039;FAQs for Users&#039;&#039;&#039;&amp;lt;BR&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;1) What tissues are compatible?&#039;&#039;&#039;&amp;lt;br&amp;gt;&lt;br /&gt;
Visium HD (probe-based) supports human and mouse tissues in FF, FxF, and FFPE formats. Visium HD 3&#039; (polyA) supports fresh frozen tissue from any species. 10x Genomics maintains a [https://www.10xgenomics.com/support/spatial-gene-expression-hd/documentation/steps/tissue-prep-for-fresh-frozen/visium-hd-spatial-gene-expression-tested-tissues-fresh-frozen list of tested tissues]. Additional optimization may be required for tissues with high fat content. Consult BMC staff before starting.&lt;br /&gt;
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&#039;&#039;&#039;2) How do I prepare and submit tissue?&#039;&#039;&#039;&amp;lt;br&amp;gt;&lt;br /&gt;
Tissue sectioning and H&amp;amp;E staining are performed by the [https://ki-sbc.mit.edu/histology Hope Babette Tang (1983) Histology Facility] at the Koch Institute. Coordinate with both the Histology Facility and BMC staff before your first submission. FF tissue should be embedded in OCT and stored at −80°C; FFPE blocks at room temperature.&lt;br /&gt;
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&#039;&#039;&#039;3) What is the resolution?&#039;&#039;&#039;&amp;lt;br&amp;gt;&lt;br /&gt;
Visium HD captures data at 2 µm bin resolution (single-cell scale). SpaceRanger aggregates into 8 µm and 16 µm bins for higher sensitivity by default; custom bin sizes are also supported. Cell segmentation based on H&amp;amp;E imaging is available in the Loupe Browser pipeline.&lt;br /&gt;
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&#039;&#039;&#039;4) How much sequencing is needed?&#039;&#039;&#039;&amp;lt;br&amp;gt;&lt;br /&gt;
400–800M reads per tile is recommended for Visium HD. Sequencing is submitted separately via iLabs. Compatible platforms include NovaSeq SP, NovaSeqX, and Element AVITI. Discuss read depth with BMC staff based on your tissue type and goals.&lt;br /&gt;
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&#039;&#039;&#039;5) Does Visium HD require permeabilization optimization?&#039;&#039;&#039;&amp;lt;br&amp;gt;&lt;br /&gt;
No. Visium HD does not require permeabilization time optimization. For fresh frozen tissues new to the platform, a test array is available to verify tissue quality and placement before committing a full experiment.&lt;br /&gt;
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&#039;&#039;&#039;6) Can I detect proteins alongside gene expression?&#039;&#039;&#039;&amp;lt;br&amp;gt;&lt;br /&gt;
Yes. H&amp;amp;E or IF staining can be performed prior to CytAssist processing. Antibody panels for protein co-detection (human tissues, FFPE only) are available from 10x Genomics. Discuss your experimental design with BMC staff in advance.&lt;br /&gt;
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&#039;&#039;&#039;7) What analysis outputs are provided?&#039;&#039;&#039;&amp;lt;br&amp;gt;&lt;br /&gt;
The BMC delivers FASTQ files, aligned BAM files, the spatial feature-barcode matrix (raw and filtered at 2, 8, and 16 µm bins), and a Loupe Browser file. Downstream informatics support is available through BMC staff.&lt;br /&gt;
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&#039;&#039;&#039;8) Can I combine Visium HD with single-cell data?&#039;&#039;&#039;&amp;lt;br&amp;gt;&lt;br /&gt;
Yes. Visium HD data integrates with single-cell or single-nucleus RNA-seq (e.g., 10x Chromium Flex) for cell-type deconvolution. Starting from the same tissue block, nuclei can be isolated for Flex single-cell gene expression. Coordinate with BMC staff if planning a multi-modal experiment.&lt;br /&gt;
&amp;lt;br&amp;gt;&amp;lt;br&amp;gt;&lt;br /&gt;
&#039;&#039;&#039;9) My gene of interest is exogenous (GFP, reporter, viral) or missing from the probe set. What are my options?&#039;&#039;&#039;&amp;lt;br&amp;gt;&lt;br /&gt;
The probe-based Visium HD assay does not capture exogenous sequences by default. Custom probes can be designed for detection — see 10x Genomics technical note CG000621. Additionally, a small fraction of endogenous genes are excluded from the standard probe sets due to predicted off-target activity: see the [https://www.10xgenomics.com/support/spatial-gene-expression-hd/documentation/steps/probe-sets/visium-human-transcriptome-probe-set-v2-1 human excluded gene list] (~2.1% of genes) and [https://www.10xgenomics.com/support/spatial-gene-expression-hd/documentation/steps/probe-sets/visium-mouse-transcriptome-probe-set-v2-0 mouse excluded gene list] (~1.8% of genes). Alternatively, the polyA-based Visium HD 3&#039; assay captures all polyadenylated transcripts and does not require custom probes.&lt;br /&gt;
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== AVITI24 CYTOPROFILING &amp;amp; IN SITU SEQUENCING ==&lt;br /&gt;
&amp;lt;div style=&amp;quot;background:#fff8e6; border-left:4px solid #c8960c; padding:8px 12px; margin-bottom:12px;&amp;quot;&amp;gt;&#039;&#039;&#039;Beta Service — Early Access:&#039;&#039;&#039; The AVITI24 Teton CytoProfiling and DISS services at the BioMicro Center are operational but not yet routine. Parameters, pricing, and workflows are still being established. &#039;&#039;&#039;Consultation with BMC staff is required before submitting samples.&#039;&#039;&#039;&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;!-- placeholder image left: AVITI24 instrument photo or Teton CytoProfiling schematic --&amp;gt;&lt;br /&gt;
&amp;lt;!-- replace with: [[File:AVITI24_instrument.png|thumb|left|400px]] --&amp;gt;&lt;br /&gt;
&lt;br /&gt;
The BioMicro Center provides access to the [https://www.elementbiosciences.com/products/aviti24 Element Biosciences AVITI24] platform for in situ single-cell multiomics. Using [https://www.elementbiosciences.com/products/aviti24/cytoprofiling Teton™ CytoProfiling], the instrument co-detects RNA transcripts, proteins (including phospho-proteins), and cell morphology at subcellular resolution — directly in intact cells, without library preparation. Targeted in situ sequencing via Direct In Sample Sequencing (DISS) can be run simultaneously in the same experiment. Because this is an early-access service, results and run parameters are less well-established than our established offerings. Consultation with BMC staff is strongly encouraged before designing experiments.&lt;br /&gt;
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{|&lt;br /&gt;
 |- style=&amp;quot;vertical-align: top;&amp;quot;&lt;br /&gt;
 |style=&amp;quot;width: 500px;&amp;quot;|&lt;br /&gt;
&lt;br /&gt;
=== PLATFORM SPECIFICATIONS ===&lt;br /&gt;
 {| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
  ! Instrument&lt;br /&gt;
  | Element Biosciences AVITI24&lt;br /&gt;
  |-&lt;br /&gt;
  ! Technology&lt;br /&gt;
  | Teton™ CytoProfiling (ABC Sequencing)&lt;br /&gt;
  |-&lt;br /&gt;
  ! Flow cell formats&lt;br /&gt;
  | 1-well · 12-well (96-well format) · 48-well (384-well format)&lt;br /&gt;
  |-&lt;br /&gt;
  ! Imageable area&lt;br /&gt;
  | Up to 10 cm² per flow cell side&lt;br /&gt;
  |-&lt;br /&gt;
  ! Max cells per run&lt;br /&gt;
  | Up to 1 million adherent cells per flow cell&lt;br /&gt;
  |-&lt;br /&gt;
  ! Transcript density&lt;br /&gt;
  | ~100–200 transcripts per 100 µm²&lt;br /&gt;
 |}&lt;br /&gt;
&lt;br /&gt;
=== ASSAY CAPABILITIES ===&lt;br /&gt;
 {| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
  ! style=&amp;quot;width:18%&amp;quot;|&lt;br /&gt;
  ! style=&amp;quot;width:27%; background:#e8f0f8;&amp;quot;| Teton CytoProfiling&lt;br /&gt;
  ! style=&amp;quot;width:27%; background:#e8f4e8;&amp;quot;| DISS (low output) &amp;lt;BR&amp;gt;&amp;lt;small&amp;gt;&#039;&#039;&#039;available&#039;&#039;&#039;&amp;lt;/small&amp;gt;&lt;br /&gt;
  ! style=&amp;quot;width:27%; background:#f5f0f0;&amp;quot;| DISS (high output) &amp;lt;BR&amp;gt;&amp;lt;small&amp;gt;&#039;&#039;not yet released&#039;&#039;&amp;lt;/small&amp;gt;&lt;br /&gt;
  |-&lt;br /&gt;
  ! RNA&lt;br /&gt;
  | 350 transcripts &amp;lt;BR&amp;gt;&amp;lt;small&amp;gt;panel-based&amp;lt;/small&amp;gt;&lt;br /&gt;
  | Targeted genes &amp;lt;BR&amp;gt;&amp;lt;small&amp;gt;~50 nt, 1 priming event; CRISPR guides, barcodes, expressed mutations&amp;lt;/small&amp;gt;&lt;br /&gt;
  | &amp;lt;span style=&amp;quot;color:#999;&amp;quot;&amp;gt;Whole transcriptome &amp;lt;BR&amp;gt;&amp;lt;small&amp;gt;polyT prime; 100–300 nt&amp;lt;/small&amp;gt;&amp;lt;/span&amp;gt;&lt;br /&gt;
  |-&lt;br /&gt;
  ! Protein&lt;br /&gt;
  | colspan=&amp;quot;3&amp;quot; | ~18 targets &amp;amp;nbsp;&amp;lt;small&amp;gt;panels of 24; rabbit monoclonal primary; custom available&amp;lt;/small&amp;gt;&lt;br /&gt;
  |-&lt;br /&gt;
  ! Morphology&lt;br /&gt;
  | colspan=&amp;quot;3&amp;quot; | Nucleus, actin, membrane (all species) · ER, Golgi, mitochondria (human only)&lt;br /&gt;
  |-&lt;br /&gt;
  ! Panels / probes&lt;br /&gt;
  | MAPK Cell Cycle · MAPK Apoptosis · Immuno-Oncology · Neuroscience &amp;lt;BR&amp;gt;&amp;lt;small&amp;gt;mix up to 3 panels per run; consult staff for latest&amp;lt;/small&amp;gt;&lt;br /&gt;
  | User-designed probes&lt;br /&gt;
  | &amp;lt;span style=&amp;quot;color:#999;&amp;quot;&amp;gt;User-designed or polyT probe&amp;lt;/span&amp;gt;&lt;br /&gt;
 |}&lt;br /&gt;
Teton CytoProfiling and DISS (low output) can be run simultaneously in the same experiment. Custom antibody add-ons require unconjugated rabbit monoclonal primaries. Consult BMC staff for current options.&lt;br /&gt;
&lt;br /&gt;
=== SAMPLE REQUIREMENTS ===&lt;br /&gt;
 {| class=&amp;quot;wikitable&amp;quot; border=1&lt;br /&gt;
  ! Supported&lt;br /&gt;
  | Monolayer cell culture (adherent); pelleted cell suspension (spun down and resuspended)&lt;br /&gt;
  |-&lt;br /&gt;
  ! Not yet supported&lt;br /&gt;
  | Complex 3D culture, organoids, tissue sections — contact BMC to discuss future availability&lt;br /&gt;
  |-&lt;br /&gt;
  ! Cell density&lt;br /&gt;
  | Consult BMC staff; optimized per cell type&lt;br /&gt;
  |-&lt;br /&gt;
  ! Species&lt;br /&gt;
  | Human (all current fixed panels); other species — consult staff regarding panel compatibility&lt;br /&gt;
 |}&lt;br /&gt;
&lt;br /&gt;
=== SUBMISSION ===&lt;br /&gt;
&amp;lt;!-- iLabs link stub: replace once service item is created --&amp;gt;&lt;br /&gt;
&amp;lt;!-- [https://mit.ilabsolutions.com/service_item/new/XXXX iLabs (assisted service)] --&amp;gt;&lt;br /&gt;
Submission via iLabs is not yet available. Contact BMC staff directly to discuss your experiment before scheduling. &#039;&#039;&#039;Consultation is required for all AVITI24 Teton and DISS submissions.&#039;&#039;&#039;&lt;br /&gt;
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=== AVITI24 Teton CytoProfiling at BMC ===&lt;br /&gt;
[[File:AVITI24_Boyer_cardiomyocytes.jpg|thumb|right|300px|Cytoprofiling of cardiomyocytes. Collaboration with the Boyer Lab.]]&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;About the Service&#039;&#039;&#039;&amp;lt;BR&amp;gt;&lt;br /&gt;
The AVITI24 at BMC supports Teton™ CytoProfiling — an in situ multiomics approach that co-detects RNA transcripts, proteins (including phospho-proteins), and cell morphology directly inside intact cells on the flow cell. Each run cycles through Cell Painting (morphology imaging), antibody scoring, and in situ sequencing rounds without any library preparation. A full run takes 24–48 hours end-to-end with approximately 45 minutes of hands-on time, capturing ~100–200 transcripts per 100 µm² at subcellular resolution.&lt;br /&gt;
&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
The platform is designed for cells in monolayer culture or spun-down suspension. It profiles up to 350 RNA targets, ~18 protein targets, and 6 morphology channels simultaneously in a single experiment — giving a co-registered multiomic readout per cell that would otherwise require separate assays on separate instruments.&lt;br /&gt;
&amp;lt;BR&amp;gt;&amp;lt;BR&amp;gt;&lt;br /&gt;
Targeted in situ sequencing (DISS, low output) can be layered onto any Teton run. This uses a user-designed probe (~50 nt, 1 priming event) to detect specific sequences in situ — useful for CRISPR guide detection, barcode readout, or expressed mutation calling. Note that reads are too short for TCR/BCR sequencing; consult BMC staff if your application involves immune repertoire.&lt;br /&gt;
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&#039;&#039;&#039;Available Assays&#039;&#039;&#039;&amp;lt;BR&amp;gt;&lt;br /&gt;
BMC supports all four currently available fixed Teton panels (MAPK Cell Cycle, MAPK Apoptosis, Immuno-Oncology, Neuroscience). Up to three panels can be combined per run, giving up to 350 RNA targets and ~18 usable protein targets in a single experiment. Custom antibody add-ons are possible using unconjugated rabbit monoclonal primary antibodies — no conjugation or barcoding required. Discuss panel selection with BMC staff; reagent availability and lead time vary.&lt;br /&gt;
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&#039;&#039;&#039;Data Outputs&#039;&#039;&#039;&amp;lt;BR&amp;gt;&lt;br /&gt;
The BMC provides core analysis outputs including:&lt;br /&gt;
* Cell-by-gene expression matrix (350 transcript targets per panel)&lt;br /&gt;
* Cell-by-protein parameter data (~18 antibody targets)&lt;br /&gt;
* Morphology feature data per cell (nucleus, actin, membrane; ER, Golgi, mitochondria for human)&lt;br /&gt;
* Spatial maps with morphology channel overlays&lt;br /&gt;
* [https://www.elementbiosciences.com/products/elembio-catalyst ElemBio Catalyst / CytoCanvas]-compatible files for interactive visualization and downstream analysis&lt;br /&gt;
&amp;lt;BR&amp;gt;&lt;br /&gt;
Additional informatics support is available through BMC staff.&lt;br /&gt;
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&#039;&#039;&#039;Pricing&#039;&#039;&#039;&amp;lt;BR&amp;gt;&lt;br /&gt;
Pricing for AVITI24 Teton CytoProfiling and DISS is not yet finalized. See [[BioMicroCenter:Pricing]] for updates as pricing is established. Sequencing-only runs on the AVITI24 are charged at standard AVITI24 sequencing rates.&lt;br /&gt;
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&#039;&#039;&#039;Service Status&#039;&#039;&#039;&amp;lt;BR&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;background:#fff8e6; border-left:4px solid #c8960c; padding:8px 12px; margin-top:8px;&amp;quot;&amp;gt;This service is in &#039;&#039;&#039;beta&#039;&#039;&#039;. The instrument is operational and BMC has completed initial runs, but experimental parameters are still being validated. We welcome early users willing to work collaboratively with BMC staff to establish protocols.&amp;lt;/div&amp;gt;&lt;br /&gt;
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|}&lt;/div&gt;</summary>
		<author><name>Asoberan</name></author>
	</entry>
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